LOCUS       DAOPUZ010000001        12606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_5076_length_12606_cov_3.215043, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000001 DAOPUZ010000000
VERSION     DAOPUZ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 12606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12606
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5076_length_12606_cov_3.215043"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="PKN79_00005"
     CDS             <1..1414
                     /locus_tag="PKN79_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HNZ94186.1"
                     /translation="VLMESAALQCWFALSRLIERSSSLLFLIGGGNNGADGLAIARHA
                     YHAGVRKITVLHTATRYTDEHAFQQTLLKEYPIEQIWLDDTHLDGLSSHDWIIDAITG
                     LGLNGPLNEKLHDLISWVNHSDASVFSVDIPSGLGDRCAVSNLCISSDHTVALGSFTQ
                     AHYHPLARSRCGNLRIANPSFPLALLDSLNVIATVDDEPPAIPALNRSAYKNTRGSIA
                     IFGSSEKYTGAARLAANAAFTARSGLVTLFCDPEIHEICASNTLSVMVRSYRGEPVEG
                     FDAILAGPGWGEGREEILKVLLSMSTPLVIDADGIRSLARLIASKEYSVESRNAPMIL
                     TPHLGEMRVIGEAALGRRCFESSESPESFFSRLKAIAQTLDATIVLKSSLIHIVDPDG
                     TITILEGLNPSLGVAGSGDLLGGMITAFLGQGFSAHDSSLIAGKILFKSALMANERIG
                     YYDSETLLPYIGLAIKEAER"
     gene            1411..1821
                     /locus_tag="PKN79_00010"
     CDS             1411..1821
                     /locus_tag="PKN79_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94187.1"
                     /translation="MIQLYLLSVAYLVIGSGFLLSDTHGIKMSILFSLRFTFRNNRAV
                     RLGLIIGGFLLTIGLALFPIDPGPRIIGDLLPLINIFILSIWYLTESLQKSEADELFT
                     SVVGRYRERSRERMGYVTLVIALIHFLLPHFVLL"
     gene            1830..2213
                     /locus_tag="PKN79_00015"
     CDS             1830..2213
                     /locus_tag="PKN79_00015"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HNZ94188.1"
                     /translation="MEERNTTAGIVRQDGKLLIAKRVEGGPVGGLWEFVGGKCRHCET
                     PEEALVREFEEELGISVVVGKLLATHTFENNHVRYHLGAYEVKRVDSKPITLSVHTEI
                     AWVSFSELDAYTFPPSDQAIISQLR"
     gene            complement(2210..3796)
                     /locus_tag="PKN79_00020"
     CDS             complement(2210..3796)
                     /locus_tag="PKN79_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNZ94189.1"
                     /translation="MDERKRQIERGETILGIELGSTRIKAVLIDSHNEPIAQGGHTWE
                     NQLVDGVWTYSLEAVEEGLQASFKSLNDDIKAQYNVVLKRTKALAISAMMHGYLAFDN
                     EDNLLVPFRTWRNTMTEQAAEDLSKLFDYPVPERWSISHLYQAILNKEEHVPRIAKIT
                     TLAGYVHYRLTGKHLLGIGDASGMFPVDPKTKGYDRTMVKQFASLHTHPWRLEELLPA
                     PLSAGEEAGVLTKEGALFLDPSGTLEAGIPLAPPEGDAGTGMVATNSVAKRTGNVSAG
                     TSVFAMIVLEKELSRSYNKFIDLVTTPDGSLVAMSHANNCTGEYDAWIALFDEVLHAV
                     GHSVPKAKLYDNLLGKALEGDYDGGGLLAYNYLSGETMTELYEGRPLFVRKAESSFTL
                     ANFMRTQLYTALGVLRIGMDILFEQEGVSIDSITGHGGFFKTALVGQKMMASALHTPV
                     ATLKSAGEGGAWGIALLASYLVNKKGRTLAEFLDQDVFAGAERTIVEATGAEIEGFNK
                     FMERYKEGLGIERAAVEFLR"
     gene            complement(3799..5097)
                     /locus_tag="PKN79_00025"
     CDS             complement(3799..5097)
                     /locus_tag="PKN79_00025"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNZ94190.1"
                     /translation="MNRRKPNVIAIVLGGGKGTRLYPLTMDRAKPAVPFGGKYRLVDI
                     PISNCINSGIKKIYILTQFNSASLHNHISNTYIFDTFSKGFVEILAAEQTIKTETWYE
                     GTADAVRKNLRHFRDQEADYYMILNEDQLYRMDLMDMLNRHIDAKAEFTIAAKPISRA
                     QATGLGIIGCDDKGIITSFIEKPAPDVDITSYKVGQKFMKESLGVSVTRENEYLASMG
                     IYIFNSKTMEEVLDNKKTDFGKEIIPQEISKRRAAAYLFDGFWEDIGTVKAFYETNLD
                     LASINPMFNFYDEEMPIYTHRRHLPATKINFCNISNSLTSEGSIITNAYIVNSIIGVR
                     TLIESGASLDGVYCMGASRYETDEEKEQNAKKGIPNIGIGRGTIIRKAIIDQNARIGD
                     GCRIGIDDIPRQEGDFPMYSIHDGIIVINKNAVIKNGTVM"
     gene            complement(5094..6551)
                     /locus_tag="PKN79_00030"
     CDS             complement(5094..6551)
                     /locus_tag="PKN79_00030"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNZ94191.1"
                     /translation="MKICMVSSESVPFSKSGGLADVVGALSKALASLGEEVLVVLPHY
                     GFMTDELIPTDIEHTVSLLGSEERVTYAKLSEPGVEYYFVQHPFFTESKGIYGPTSSS
                     SYEHNHLRYTLQAKASLELLKILGWKPDIIHAHDWTAGFLPYLVKRDSSGFFKKTKTV
                     FSIHNLAYQGEFSRLDLLETDIEVDPRFFVGSGPKKRANMLKTGLEFADTLSTVSPTY
                     AKEIQTPEYGCGLEGLLQRRKDDLVGIINGIDIDEWNPENDRLLDYSFSIDDLSNKAR
                     LKRQIQEEFNLEVNAGIPLFSMISRLVSQKGFVELLEGEVSALEELLTTEEIQVVVLG
                     TGDWELEKKFTELGARFKNLSVNILFSNRAAHLLEAGSDFFLMPSRYEPCGLNQFYSL
                     RYGTLPVARRTGGLADSITDLDEDPQRGTGILFDSMSGRGILEAVRRALAWWKKGRTV
                     IEPIRKRAMAYDSSWTRSARSYQELYRKTQGGFTK"
     gene            complement(6600..7544)
                     /gene="trxB"
                     /locus_tag="PKN79_00035"
     CDS             complement(6600..7544)
                     /gene="trxB"
                     /locus_tag="PKN79_00035"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNZ94192.1"
                     /translation="MVEQEILVLGSGVAGMAAGQYAARAGRTVTIMESIAPGGQTMYI
                     DLIENYPGIEVPVSGAEIALKFQSQAETFGAKIVYETATAIRKEGDDFLVETASGTTY
                     KAKAVIYATGAKHRHLEIEGEEAYAGKGVSYCGTCDGPFFRGKRILVVGGGDTAITDA
                     IYLSKLTDHITIAHRKDRFRAQDNLVKQIERNPHVEFLMEHTLQEIKGDGNKVTSVIL
                     KDLRQDREYEREFDAVFIFVGIIPETNLLKGSYDVLTEDGWVITNERMETSVEGLFAA
                     GDVRDTVFRQLITAASDGAVAAHCASSYIDELEGRAYT"
     gene            complement(7549..7833)
                     /locus_tag="PKN79_00040"
     CDS             complement(7549..7833)
                     /locus_tag="PKN79_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94193.1"
                     /translation="MDGIQIGESSDLSPDEIASLIAPHPSITLLGSDGTLLAKRLADP
                     KVAVFEAHRQNLGVELADRARKHYLETGADPLDQGPTYIRKSDAELALKE"
     gene            complement(7787..8221)
                     /gene="tsaB"
                     /locus_tag="PKN79_00045"
     CDS             complement(7787..8221)
                     /gene="tsaB"
                     /locus_tag="PKN79_00045"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNZ94194.1"
                     /translation="MIILGCDTSTKTAHLFLGEYEEDQLSWYETTSVVWNNTHSEQLL
                     DRLLLMCSDHSIELSELDLLCASSGPGSFTGLRIALATMKGLALGLNKPLVSVPTLDL
                     FQDRPIHRRSMPCGHRCKKTALLHRAVRGWNPDRRKQRSESG"
     gene            complement(8218..8637)
                     /gene="tsaE"
                     /locus_tag="PKN79_00050"
     CDS             complement(8218..8637)
                     /gene="tsaE"
                     /locus_tag="PKN79_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNZ94195.1"
                     /translation="MIVRTTGEKETIRVGYALGETLAKGAVVSLRGSLGVGKTVFAKG
                     IAQSLGIREAIVSPTFTLIQEYEGTLPLYHMDLYRIDSVEEFELIGGEEYLYGEGITL
                     IEWSEKIQELLPEGTISVTITLAEDGSREIRIEGSTL"
     gene            complement(8634..9818)
                     /locus_tag="PKN79_00055"
     CDS             complement(8634..9818)
                     /locus_tag="PKN79_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ94196.1"
                     /translation="MHEALNHVIFISLPPSMERKIGSFTIDPAIRLPVALADDHTAID
                     ERSISIERIVSGMLKVLAYDIDHQHAPYYREFVLAAQPDAVKELTIAAIAQQQKGNLT
                     FAEELFLTVCHLSPQSATYINLATLYGHRATEDSSKGETYDFYQQKALNTLVEGLSVL
                     GDDEALLKEIGFFHLYQGNVEIARDYLVRYVDLAGESKEKRHTMKILKDIETKLAGEQ
                     RLMQAYDAIHMNREEEALSLLNRYIEAYPKVWNAYFLKGWALRRLERFPEAQDALIQA
                     LELESHSSDIYNELALCALEAGNEELAKSYLGTALDLDEENITFMSNLAYLHLRSGEL
                     EEARHLLEQARSLDGEDPLIQRLMEDYTHQSGDTLGDVIIEEYLENEEIADKLKHGAK
                     EI"
     gene            complement(10142..10501)
                     /locus_tag="PKN79_00060"
     CDS             complement(10142..10501)
                     /locus_tag="PKN79_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94197.1"
                     /translation="MSVIDTVRLLELRTKKAAGLIALLRREKEELQQRFDLVLAHNRE
                     LEGYIEAYTTSEKLVEQSIATAMATLDTIDGLDEIQVFDDASLELEAADTFTGADGGL
                     SSEDVDWDALLDDLPEL"
     gene            complement(10518..10877)
                     /gene="rplT"
                     /locus_tag="PKN79_00065"
     CDS             complement(10518..10877)
                     /gene="rplT"
                     /locus_tag="PKN79_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNZ94198.1"
                     /translation="MPRAIDGTKHKDRRKKILKLAKGHRGRRSTNYRVAKDSVEKAGQ
                     HAYVGRKLRKRDFRRLWIVRINAAVQAEGLNYSQFMHGMKLANIEVNRKALSNMAIED
                     KAAFKTLVDQVKVALAK"
     gene            complement(10889..11086)
                     /gene="rpmI"
                     /locus_tag="PKN79_00070"
     CDS             complement(10889..11086)
                     /gene="rpmI"
                     /locus_tag="PKN79_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004790647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNZ94199.1"
                     /translation="MPKMKTRKSAAKRFKVTAGGKILYKKQGLRHILTKKSSKRKAHL
                     RSTGVLSDVEARRVKSMLPYE"
     gene            complement(11164..11712)
                     /gene="infC"
                     /locus_tag="PKN79_00075"
     CDS             complement(11164..11712)
                     /gene="infC"
                     /locus_tag="PKN79_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNZ94200.1"
                     /translation="MATRDLRVNRQIRAREVLVIDEDGNQKGIMSSFDAIELAEQAGL
                     DLVEVSPNANPPVCKILDYGKFRYEQEKRLRDQKRNQVITKMKEIRMQPKIEQHDLNT
                     KSKFIGDFLTEGNKVKVSIRFRGRELAHTELGKVVLDRILAQLTDNGVGYVVDRPAMM
                     EGRMMSMILSPAKTSGPKKEPQ"
     gene            complement(11864..>12606)
                     /locus_tag="PKN79_00080"
     CDS             complement(11864..>12606)
                     /locus_tag="PKN79_00080"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNZ94201.1"
                     /translation="DIPIVLHLDHGDSFETCKDCIDNGFSSVMIDGSHLPYEENVALT
                     KKVVEYAHKHDVTVEGELGVLAGIEDEVSHEKSTYTKPEQVVDFVSRTGVDSLAISIG
                     TSHGANKFNPAHCTVNEEGILIPPPLRFDILEEIERRLPGFPIVLHGSSSVPQEYVKI
                     INKYGGKLKDTIGIPEEQLRRAAKSAVCKVNIDSDGRLAMTATIREILHNNPGEFDPR
                     KYLGPARDELKKMYIHKNTNVLGSANQA"
BASE COUNT         2587 a         3432 c         3382 g         3205 t
ORIGIN      
        1 ggtactgatg gaaagcgccg cccttcagtg ctggtttgca ctgtctcgac tcattgagcg
       61 gtcgtcgtcg ctgctctttc tcatcggcgg gggaaacaac ggagctgacg ggctggcgat
      121 cgcccgtcat gcgtaccacg ccggggtgcg gaagatcacg gtgcttcata ccgccacccg
      181 gtacactgat gaacacgctt ttcagcaaac gctcctcaag gagtatccga tcgagcagat
      241 atggttggat gatacacatc ttgacggcct ctcctcccat gactggatca tcgatgcgat
      301 caccggcctc ggcttgaacg gcccgttgaa tgagaagctc cacgatctca tttcatgggt
      361 caatcattcc gatgcttcgg tgttttccgt tgacatcccc tccggtctgg gcgatcgatg
      421 cgccgtatcg aacctttgca tctcctccga tcatacggtg gctctcggct cgttcaccca
      481 agcccattac catcctcttg cccgcagccg ctgtgggaac ctccggattg ccaatccctc
      541 gtttcccttg gcgctcctcg actcgctgaa cgtcatcgcc acggtcgatg acgagccgcc
      601 tgcgataccg gctctcaatc gctccgctta caagaacacc cggggaagca tcgcaatctt
      661 cggttcaagc gagaaataca ccggggcggc ccgtcttgcg gcgaacgcgg cgttcaccgc
      721 tcgaagcggc ctggtgaccc tgttctgcga tcccgagatc cacgagatct gcgcatcgaa
      781 taccctcagc gtcatggttc gttcctaccg gggggaaccg gtggaaggct ttgatgcgat
      841 cctggcgggg ccggggtggg gggagggacg ggaagagatt ctgaaggttc ttctgtccat
      901 gtcgactccg ctcgtcatcg atgccgacgg aatccgttcc cttgcccgtc tcatcgcttc
      961 gaaggaatat tcggttgaaa gcaggaacgc tccgatgatt ctcacccccc atctcgggga
     1021 gatgcgggtc atcggggagg cggctctggg caggagatgc tttgaatcaa gcgagagccc
     1081 tgaatcgttc ttcagtcggc tgaaagctat tgctcaaacc cttgacgcga cgatcgtgct
     1141 gaaaagcagc ctgatccaca tcgtggatcc cgacggaacg atcacgattc tggaggggct
     1201 caatccttcc ttgggagtcg ccggaagcgg ggacctgctc ggcgggatga tcaccgcctt
     1261 cctcgggcaa gggttcagtg cccatgattc gagtctcatc gccggcaaga ttctctttaa
     1321 gagcgccctg atggcgaatg agcggatcgg gtattatgac agtgaaaccc tgcttccgta
     1381 catcggtctt gccataaagg aggcggagcg atgattcagc tgtatcttct ttcggtggcg
     1441 tacctcgtca tcggctcggg gttcctgctt tccgataccc acggaatcaa aatgagcatt
     1501 ctgttttctc tgcggttcac ctttcggaac aaccgggcgg ttcgcctggg tctgatcatc
     1561 ggcggctttc tgctgaccat cgggcttgcc ctctttccca tcgatccggg acctcgaatc
     1621 atcggagacc ttctgccact gatcaatatc ttcatactga gcatctggta ccttaccgaa
     1681 tctcttcaaa aatctgaagc cgacgagctc tttacgagcg tggtgggccg gtatcgggag
     1741 cgcagccggg agaggatggg atatgtcacc ttggtgatag cacttattca ttttctcctt
     1801 ccccatttcg tgttattgtg agatgcagga tggaagagcg aaataccacg gcaggcatcg
     1861 tcagacagga tgggaagctc ctcattgcaa aacgggtcga aggcggaccg gtgggaggtc
     1921 tatgggagtt tgtcggggga aaatgccgtc attgcgaaac gccggaggaa gctctggttc
     1981 gggagtttga agaggagctg ggcatctccg tcgtcgtggg aaagcttctg gccacccaca
     2041 cctttgaaaa caaccatgtc cgataccact tgggcgccta tgaggtgaag cgggtggact
     2101 caaaaccaat cactctgagt gtgcatactg aaatcgcctg ggtttccttc tcggagttgg
     2161 atgcgtacac ctttcctccg agtgaccagg cgattatctc acagttgcgt taacgcaaaa
     2221 actcgacagc ggctcgttcg attccaagac cttccttata gcgctccatg aatttgttga
     2281 atccttcaat ctccgcgccg gtcgcttcga cgatcgttcg ctccgcaccg gcaaacacat
     2341 cctgatcgag gaactcggcg agcgttcggc cttttttatt gacgagatag gatgcaagca
     2401 acgcgattcc ccacgctccg ccttcaccgg cgcttttaag tgtggccacc ggagtgtgca
     2461 gagcgctggc catcatcttc tgtccaacca atgcggtctt gaagaagccg ccatgaccgg
     2521 tgatcgaatc gatgctcacc ccctcttgtt caaagaggat gtccattccg atcctcagga
     2581 cgcccaaggc ggtgtagagt tgggttctca tgaagttggc gagggtgaag gagctctccg
     2641 ccttgcgaac gaagagaggt ctgccttcat acagctcggt catcgtttct cccgagaggt
     2701 agttgtacgc gagcagtccg ccgccgtcgt aatctccttc aagagctttg ccgagcagat
     2761 tgtcatacag cttcgccttg ggtacgctgt gccccacggc gtggagcact tcatcaaaca
     2821 gagcgatcca cgcatcgtat tcaccggtgc agttgttggc gtggctcata gcaacgagcg
     2881 agccgtcggg ggtggtgacg aggtcgatga atttgttata cgaacgggac agctccttct
     2941 cgagcacgat catcgcaaag acgctcgttc ccgcggacac gttcccggtt cgcttggcca
     3001 cgctgttcgt cgcgaccatt cccgttcccg catccccttc gggcggagcc agagggatgc
     3061 ctgcttccaa ggtcccgctc ggatcaagga agagagcccc ctctttggtg aggactcccg
     3121 cctcctctcc cgctgaaagc ggagcaggca agagctcttc caacctccaa ggatgggtat
     3181 gcaatgaagc aaactgcttc accatcgtcc ggtcataccc cttggtcttc ggatcgaccg
     3241 ggaacatccc cgaagcgtct ccgattccca agaggtgttt cccggtcagt cgataatgaa
     3301 cgtatcccgc caaggttgtg atcttggcga ttcttggaac gtgctcttcc ttgttcagga
     3361 ttgcctgata gagatgactg atcgaccaac gctcgggaac cggataatca aaaagcttgg
     3421 agagatcctc cgccgcttgt tcggtcatcg tgttccgcca cgttcggaag ggaacaagaa
     3481 ggttgtcctc gttatcaaac gccagatagc cgtgcatcat cgcactgatc gccagagctt
     3541 tcgttcgctt caacaccaca ttgtattggg ctttgatatc atcattcagt gatttgaatg
     3601 acgcctgcaa gccctcttcg accgcctcca agctgtaggt ccacactccg tcgacgagct
     3661 ggttctccca cgtgtgaccg ccttgagcga tcggctcgtt gtgcgagtcg atgaggaccg
     3721 ccttgattcg tgttgatccg agttcaatgc ctaaaattgt ttctccacgt tcaatctgtc
     3781 tctttcgttc atccattact acatcaccgt tccattttta atgactgcgt ttttattgat
     3841 gacgatgatt ccgtcgtgga tggaatacat cgggaagtct ccttcctgac ggggaatatc
     3901 gtcgatgccg attcggcatc cgtcaccgat ccgggcattc tggtcgatga tcgccttgcg
     3961 aatgatcgta ccccggccga tgccgatatt cggaatgccc ttctttgcgt tctgctcttt
     4021 ctcctcatcc gtttcgtagc gggaggcgcc catgcaatac actccgtcga gggaagctcc
     4081 cgattcgata agggttcgaa caccgatgat cgagttcacg atatacgcat tggtgatgat
     4141 cgagccttca ctggtcagcg agttggagat gttgcagaag ttgatcttcg ttgccggcag
     4201 gtgtcggcgg tgggtgtaga tcggcatctc ctcatcatag aagttgaaca tcgggttgat
     4261 cgaagcaagg tcgaggtttg tttcatagaa cgccttgacc gttccgatat cttcccagaa
     4321 gccgtcaaag aggtaggcgg cggcgcggcg ctttgaaatc tcttggggaa tgatctcttt
     4381 tccgaaatcg gtcttcttat tgtccaggac ctcttccatc gtcttgctgt tgaagatgta
     4441 gattcccatc gaagccagat actcgttttc acgggtgact gaaactccga gcgattcctt
     4501 catgaacttc tgacccacct tgtatgaggt aatatccaca tcgggagcgg gcttttcgat
     4561 gaagctggta ataatgccct tatcatcaca gccgatgatc cccaagccgg tcgcctgagc
     4621 gcggctgatc ggtttggcgg cgatggtgaa ctccgctttg gcatcgatgt gccggttcag
     4681 catgtccatc agatccattc gatacagctg gtcctcgttg agaatcatat agtaatctgc
     4741 ttcctgatcg cggaagtggc gcaggttctt gcgcaccgcg tcggcggttc cttcatacca
     4801 ggtctcggtt tttatcgtct gctccgcggc gagaatctcg acgaaccctt tggagaaggt
     4861 atcgaagata taggtgtttg agatgtggtt gtgcagcgac gccgagttga actgagtaag
     4921 aatataaatt ttcttaatcc cgctgttgat gcagtttgag atcggaatat ccacgaggcg
     4981 atattttccg ccaaatggaa ctgcgggctt tgcccgatcc attgtcaagg gataaagacg
     5041 agtacccttt cctccgccca gtacgatagc aatgacattt ggctttcgac gattcatttg
     5101 gtaaaacccc cctgtgtttt gcgatagagc tcttggtagg aacgggccga gcgggtccac
     5161 gaactgtcgt aggccatcgc tcgttttcgg attggttcaa tgaccgtcct tccttttttc
     5221 caccaagcaa gggctctcct tactgcttcc aatataccac gaccgctcat cgagtcaaag
     5281 aggattccgg ttcctctttg aggatcttca tccaaatcgg taatcgaatc cgccaatcct
     5341 ccggttctcc tggctacggg aagcgtcccg tagcgaagac tgtaaaactg gttcagtccg
     5401 cacggctcat accggctggg catgagaaag aagtcgcttc cggcttcgag aaggtgagcg
     5461 gcccggtttg agaacagaat attcaccgaa aggttcttga acctcgctcc aagctcggtg
     5521 aatttttttt caagctccca atcgcccgta ccgaggacga cgacttgaat ctcttctgtt
     5581 gtgagcagtt cctccaacgc cgagacctct ccttccagca attcgacaaa tcccttttga
     5641 ctcaccaaac ggctgatcat cgaaaacagc ggaataccgg cattgacctc aaggttgaat
     5701 tcttcctgaa tttgccgttt gaggcgagct ttgtttgaca gatcatcgat ggagaaggag
     5761 tagtcgagca gtcgatcgtt ttcgggattc cactcgtcaa tgtctatgcc gttgatgatt
     5821 cccacgagat cgtccttccg gcgctggagc aacccctcca gtccacagcc gtactcggga
     5881 gtttggatct ctttggcata ggttgggctg acggtggaaa gggtatcggc gaactccaac
     5941 ccggttttca gcatattggc tcgttttttc ggaccgctgc cgacaaagaa acggggatcg
     6001 acctcgatat ccgtttccag aagatcgaga cggctgaatt cgccttgata ggcgaggttg
     6061 tgaatcgaga agaccgtctt ggttttcttg aagaagccgg atgaatcccg cttcacgagg
     6121 tagggcagga agcctgcggt ccaatcatgg gcgtggatga tatccggttt ccacccaagg
     6181 atcttcaaga gctccaaaga cgcttttgcc tgcagcgtgt atcgaagatg gttgtgttcg
     6241 taggatgatg aggaggtcgg cccatagatg cctttcgatt cagtaaaaaa cgggtgttgg
     6301 acaaagtagt attcaacacc cggttctgaa agctttgcat atgtgacccg ttcttcactc
     6361 cccaacaaag agacggtatg ctcgatatcg gttggaatca actcatcggt catgaaccca
     6421 tagtggggca atacgacaag tacttcttca ccaagagatg ccagagcttt tgatagggcg
     6481 ccgacaacat ccgccaaccc tccgcttttg gagaagggaa ccgattcact ggagaccatg
     6541 catatcttca tagtcccatt agaacacctt aaccgttctt gcgtcaagga aattgggttt
     6601 tacgtatatg ctctgccttc caattcatcg atgtacgagc ttgcgcagtg tgcagcaacc
     6661 gctccgtcgc tcgccgcggt gatcaactgg cggaagacgg tgtcacgaac gtctccggcg
     6721 gcgaagagcc cctcgacgga tgtttccatc cgttcgttgg taatgaccca gccatcctcg
     6781 gtgaggacat cgtaagaacc cttgagaagg ttcgtctcgg ggatgatccc cacgaagatg
     6841 aagacggcgt cgaattcccg ctcatattcg cgatcctgcc tcaaatcctt gaggatgacc
     6901 gaggtgactt tatttccgtc gccttttatc tcttgaagcg tgtgctccat caggaactcg
     6961 acgtgcgggt tgcgctcgat ctgcttgacc aggttgtcct gcgcccggaa tcggtctttg
     7021 cggtgggcga tggtgatatg gtcggtcagc ttggaaaggt agatcgcatc ggtgatggcg
     7081 gtatcgcctc cgccgacgac gagaatccgc ttgcctcgga aaaaggggcc gtcgcacgtt
     7141 ccgcagtagg aaacgccctt gccggcgtag gcttcttcac cttcaatctc caggtggcgg
     7201 tgtttcgctc cggttgcata gataacggct ttggctttgt aggtggtgcc gcttgcggtc
     7261 tccacgagga agtcatcacc ttcctttctg attgccgtgg cggtttcata gacgatcttc
     7321 gctccgaagg tctccgcttg actttggaac ttcagcgcga tctccgctcc acttacggga
     7381 acctctattc ccggatagtt ttcaatcaga tctatataca tcgtctgtcc accgggggcg
     7441 atcgattcca taatggtgac cgttctgccc gcccgggcgg catactggcc ggccgccatg
     7501 ccagccacac ccgaaccgag gaccaaaatc tcctgctcaa ccatgttctt actccttcaa
     7561 tgcaagttca gcatcgcttt tacgaatata tgtgggcccc tgatccaaag gatcggcgcc
     7621 tgtctcaaga taatgctttc ttgccctgtc ggctagctcc actccgagat tctgacggtg
     7681 agcctcaaac actgcgacct tcggatctgc cagccgcttc gcaaggagag ttccgtcgct
     7741 gccaagcagg gtaatggagg ggtggggggc gatgagcgaa gcgatttcat ccggactcag
     7801 atcgctgctt tctccgatct ggattccatc cacgaacagc gcggtgtaaa agcgctgttt
     7861 ttttgcatcg atgaccgcaa ggcatgctcc gccgatgaat cgggcgatct tgaaagagat
     7921 ccagcgtggg gacgctgacc agcggtttgt tcaatccgag ggcgagcccc ttcatcgtgg
     7981 caagggcgat cctcaagccg gtgaaggatc ccggaccgct gcttgcgcag aggaggtcca
     8041 attcggaaag ttcgatggaa tgatcggagc acatcaaaag cagccgatcc agcagttgtt
     8101 cgctgtgggt gttgttccag accaccgagg ttgtctcata ccacgagagt tgatcctctt
     8161 catattcgcc gagaaagagg tgggccgttt ttgtagaggt gtcgcaaccg agaataatca
     8221 tagcgtgctt ccttcaattc ggatctcccg cgagccgtcc tcggcgaggg tgatcgtcac
     8281 ggagatcgtc ccttcgggaa gaagctcttg gatcttttca ctccattcga tcaacgtgat
     8341 cccctcaccg tagagatact cctccccccc gatcagctca aactcctcca cgctgtcgat
     8401 ccggtagaga tccatgtgat agagggggag ggttccttcg tactcctgga tcagggtgaa
     8461 ggtggggctg acgatcgctt cccggattcc gaggctctga gcgatcccct tggcaaagac
     8521 cgtcttccct acgccgagcg atccccgcag agagacgacc gcgcccttcg caagggtttc
     8581 tccaagagca tatccgaccc ggatcgtctc cttttcccct gttgtccgaa cgatcatatc
     8641 tccttggctc catgtttcag cttgtcggcg atctcttcgt tctccaagta ctcttcgatg
     8701 atgacatccc ccagcgtgtc accgctttgg tgggtgtagt cctccatcag tcgttggatg
     8761 agggggtctt cgccgtcgag ggagcgggct tgttcaagca ggtggcgagc ctcttccagt
     8821 tccccgctcc gaaggtgcag gtacgcgagg ttgctcataa acgttatgtt ctcttcatcg
     8881 agatccaggg cggttcccag gtagcttttt gccagctcct cattgcccgc ttccaaggcg
     8941 cacagggcca gctcgttgta gatgtcgctg ctatggcttt ccagctccag ggcttggatg
     9001 agggcgtctt gggcttccgg gaagcgttca agccgccgga gcgcccaacc cttcaggaaa
     9061 taggcgttcc agaccttcgg atatgcttcg atataccggt tcagcaagga gagcgcttcc
     9121 tcctccctgt tcatgtggat cgcatcgtac gcctgcatga gccgttgttc gccggcaagc
     9181 tttgtttcga tatccttcag gatcttcatc gtgtgccgtt tttctttcga ttccccggca
     9241 agatcgacgt atcgaaccag atagtcccgg gcgatctcga cattcccctg atagagatga
     9301 aaaaagccga tctctttcag aagtgcttcg tcatcaccca gaacggacaa cccctcgacg
     9361 agagtgttca acgctttttg ctggtagaag tcatacgttt cacccttgct tgaatcctcg
     9421 gtcgcccgat gtccgtacag cgtggccagg ttgatatagg tagcggattg ggggctaagg
     9481 tgacagacgg tgagaaagag ctcttcggcg aaggtgaggt ttcccttttg ttgctgggcg
     9541 attgcggcta tcgtcagttc ttttaccgca tcgggctgag ccgccagcac gaattctcga
     9601 taatacgggg cgtgttgatg atcgatgtca tatgcaagta ctttgagcat cccgctgacg
     9661 atccgttcga tgctgatcga ccgttcatcg attgctgtat ggtcatcggc caaggcgacg
     9721 ggaagccgga tcgccgggtc gattgtgaat gatccgattt tcctttccat cgaggggggg
     9781 agggagatga agatcacatg gttcagcgct tcatgcatgg tgacattgct ccatagaaaa
     9841 tcctgcggtg ccgcaaccga agaacgctct tgaacctgtg gttcaagggg gacaccagtt
     9901 gggctgaaaa ttctgttccg ttaaaccccg aaggagttgg atcaatgcaa aacagctata
     9961 ccgcgtcccg gttgtgttag ttgagccaag agactttgtc tccaggagaa caccgcagga
    10021 atacttacaa taatcgtcag gtggcatcca cctaacaaca tggtactaca ctaaccggtt
    10081 ttgggaaaga gggacaaaaa atgggacccg aaggtcccat agacgctgtt cagacaggaa
    10141 gttacagttc cggaagatca tccagaagcg catcccaatc aacatcctcg ctgctgaggc
    10201 cgccatcggc tcccgtaaag gtgtcggcgg cttcaagctc aagggaagca tcgtcaaaga
    10261 cttgaatctc atccaagccg tcgatcgtat cgagggtggc catcgcagtg gcgatggatt
    10321 gctcgacgag cttttcactg gtggtatatg cttcgatata cccctccaat tcccggttgt
    10381 gggcaagaac caggtcgaac cgctgctgca gttcttcttt ttcccgccgt aacaaggcga
    10441 tgaggccggc ggcctttttc gttctaagct ctaagagtcg tacggtatca atgacagaca
    10501 tgcttcctcc tgcgccctta ttttgcaagg gcgaccttta cttggtcaac gagagtttta
    10561 aacgcggcct tatcctcgat agccatgttg gatagagctt tgcggttaac ttcgatgttg
    10621 gcgagtttca tcccgtgcat gaattgggag tagttcaacc cctcggcctg cacggctgca
    10681 tttatcctga caatccagag acggcggaaa tcacgctttc ggagctttcg tccgacatag
    10741 gcgtgctgtc cggctttctc tacgctgtcc tttgcgaccc tgtagttcgt gcttcgccgg
    10801 ccgcggtggc ctttagcgag cttgaggatc ttcttacgtc tgtccttgtg cttggttcca
    10861 tctattgctc ttggcatccc gatcctcctt actcgtaggg gagcatcgac ttgactcgtc
    10921 gtgcttccac atcggacagg acacctgttg agcgcaggtg ggccttgcgc ttactgctct
    10981 tcttggtcaa gatgtggcga aggccctgtt tcttgtacag gatttttcct ccggctgtca
    11041 ccttgaaccg cttggcagcg gactttcttg ttttcatctt gggcatgata gcacctcttt
    11101 ctgtgccaca gggaacaaga tacccctgga gcaacgtatt cactatccca tgcgggatac
    11161 tggttactgt ggttcttttt tcggccccga cgtcttggcc ggcgagagaa tcatgctcat
    11221 catccgccct tccatcattg ccggtcggtc aacgacgtat cctacaccat tatcggtgag
    11281 ctgggcaagt atccgatcga ggaccacttt gcccaattcg gtatgagcca attcacggcc
    11341 cctgaagcga atacttacct ttactttgtt gccttcggtg aggaagtctc cgatgaattt
    11401 gctcttggtg ttcaagtcgt gctgttcgat cttcggctgc atccgaatct ctttcatctt
    11461 ggtgatcacc tgattccgtt tttgatcccg caatcgtttt tcctgctcat agcggaactt
    11521 accataatcg agaattttgc atacaggggg gttggcgttg ggggaaacct caactaaatc
    11581 gagccctgct tgttcggcga gctcgattgc atcaaaactg ctcataatgc ccttttgatt
    11641 gccatcctca tcaatgacaa gtacttctct ggcacgaatc tgcctattga ccctcaaatc
    11701 tcttgtagcc aaatgttctc cttgtgattt aggtgttaaa accactctgc agtaatatac
    11761 ttcagcccgt atgggtgacc gaaattacta taccatagcc tcttacgatg tgtcaaagct
    11821 ggaatataag aagatagccg cccgaaggcg gccatcgatt gcgttaggct tgattggcgg
    11881 aaccaagaac gttggtgttc ttgtggatat acatcttttt cagttcgtcg cgggccggtc
    11941 cgaggtattt tcgcggatcg aattcccccg ggttgttgtg taggatttca cgaatcgtcg
    12001 ccgtcatcgc caagcggcca tcgctgtcga tgttgacctt gcacactgcg ctcttggcgg
    12061 ctctgcgaag ctgttcctcg gggattccga tcgtgtcttt cagttttcca ccgtatttgt
    12121 tgattatctt cacgtactcc tgcgggacgg aggaagatcc gtgcagaacg atcgggaacc
    12181 cgggaagccg tctttcaatc tcttccagga tatcgaagcg aagcggggga ggaatgagaa
    12241 tgccttcttc gttaacggtg cagtgggcgg ggttgaattt gttcgctccg tggctcgttc
    12301 caatggagat cgccagtgaa tcgacgccgg tccgggaaac gaagtcgacg acctgttccg
    12361 gtttggtgta ggtggatttc tcgtgcgaaa cctcatcttc gattccggca agaacgccga
    12421 gctcaccttc gacggttaca tcgtgcttgt gggcatactc gacgaccttc ttcgtcaggg
    12481 cgacgttctc ttcgtaggga aggtgggagc cgtcgatcat gacggaggag aatccgttgt
    12541 cgatgcagtc tttgcaggtc tcgaagctgt cgccatgatc caagtgcagg acgatcggga
    12601 tatcat
//
LOCUS       DAOPUZ010000002        11147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_5934_length_11147_cov_3.903805, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000002 DAOPUZ010000000
VERSION     DAOPUZ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5934_length_11147_cov_3.903805"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="PKN79_00085"
     CDS             complement(<1..1377)
                     /locus_tag="PKN79_00085"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HNZ94202.1"
                     /translation="MENRTIKVDVASEMRTSYLNYAMSVIVSRALPDVRDGLKPVHRR
                     ILYDMYEMGLRANSSYKKSARIVGDVLGKYHPHGDASVYDALVRLAQDFSLRYPVVNP
                     QGNFGSIDGDPAAAMRYTEAKMSRIGEEMLSDIQKETVDFKPNYDDSLTEPSVLPGAF
                     PFLLANGSSGIAVGMATNMAPHNLQEICAAANALIEDPEITLMELMEHIKGPDFPSGG
                     VICGMRGIVDAFSTGRGRIITRGKYEIEESARGGDVIVFTEIPYQVNKADLVKKIDDL
                     RKDGSIPMISTIRDESDRNGIRIVIELKMGAEVMVVLNQLFQRTALQSNFNVNNLALV
                     DGRPQLLGLKDMLHYYVKHRKDVVTRRTTYDLRKAQERAHILRGLKIGLEHIDEVIQI
                     IKDSPDNSIASQRLIAAFALDETQAQAIIDMRLGRLSHLESSKILEELEDLEAKIAYY
                     NELLSDEGK"
     gene            complement(1401..3431)
                     /gene="gyrB"
                     /locus_tag="PKN79_00090"
     CDS             complement(1401..3431)
                     /gene="gyrB"
                     /locus_tag="PKN79_00090"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNZ94203.1"
                     /translation="MNDGSFSNGNADLSAGAKATGAVKPPDQYSASSIQVLKGLEAVR
                     KRPGMYIGSTGIDGLHHLVYEVVDNSIDEAMAGYCDTITVTLSRDEEGRQFCTVEDDG
                     RGIPVAIHPIEKKSSLEIAMTQLHAGGKFDKNSYKVSGGLHGVGVSCVNALSIWMEVI
                     VKRDGGIFRQLYSQGNPTSEVQRIGDTDETGTKVTFSPDYTILEENAFSYETLVTRFR
                     ELSFLNKGITITVSDTRFEEPKKQTFHSEGGIVEFVSYLNEYKRTLGETPISIEGERD
                     NIKVEIALQYNDKYDEKVLTFVNNINTKEGGTHLTGFRTGLSRVINAQLQKNQKLLKR
                     YEDRLEPSDISEGLTAIVSIKIPEPQFEGQTKMKLGNSVVTGVVFSLVYEKLGNYFDE
                     FPAHAELILGKIINAALGRIAARKAREQIRKKSEGGGLPGKLADCSEKDPALCEVFIV
                     EGDSAGGSAKQGRDRRFQAILPLWGKMLNVEKAQEYRVLGNDKLQPVISTIGAGLTRY
                     NNMGKDEDLDSDVTFDITKARYHKVIIMADADVDGSHIRTLLLTFFFRYMRPLIEAGY
                     VYFAMPPLYKITIGKNVSYAYDEEARIRILEEAGPDAAKASIQRYKGLGEMNPEQLWE
                     TTMNPETRLISQVTLGDAEEADRVFSMLMGEEVEPRRAFIDANAVYVSNLDV"
     gene            3631..5028
                     /gene="dnaA"
                     /locus_tag="PKN79_00095"
     CDS             3631..5028
                     /gene="dnaA"
                     /locus_tag="PKN79_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNZ94204.1"
                     /translation="MSDQQTSYYEFWLETAKRIQETLPPQEFSTWFSRISYLSSKKGE
                     VTLAGPSQFVLDTVTTRYRSIIANTLSELTGEEIAVEFVVSDKGKKAVRPTDGDETAE
                     EDRKEKKADDLAKKISKRKSEANLNPSYEFESFVLGDNSAFAYNASLAIAKNPGSSYN
                     PCLIYGDVGLGKTHLLSSIGNYILDHTPECKVMYVTAETFTNEFIESIGSQKTQQFKN
                     KFRNVDVLLIDDIHFLQGKDSTQEELFHTFNNLYEAKKQMVFTCDRPISELKNITDRL
                     SSRFERGLNVDLQPPNYETRVAILKKKLQEHGSSLSEDIINYIAFNVVTNVRDLESSL
                     TKLVAYMDLIGEELTFEKAKQLLGILPAMANIARDTLSIGEIIKTVGDYFNVSTFEMK
                     GKKKNKSLVQPRQIAMYLARQITDFSTTEIGMEFGGRDHTTVMYANNRVESLMKGDEA
                     YAATVNKLRRQLTSS"
     gene            5252..6358
                     /gene="dnaN"
                     /locus_tag="PKN79_00100"
     CDS             5252..6358
                     /gene="dnaN"
                     /locus_tag="PKN79_00100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNZ94205.1"
                     /translation="MKFISTKEAILSEIIYTVDFTTQKNSLAITSNVYLQTLGDQLLI
                     KATDGKVGFSTSITVDVQEEGKALVLQEKLLEILRVLPDGDINFSMKDGFFTIVPVGH
                     GIDFKIRMIEADQFPALETIDNDRFFSIAQSSFLEMSEQVIFAVSDDTSRYFLCGVYL
                     ERSEGGITMVATDGRRLSIVERVFEEKLPSFAPIIVLPKFFQILRKIGTGEGAVELAI
                     TESILFARIGGRTFYITLIREQYPNFRRVIPEQQSYRCTMKIQDMEDALRRVAILIES
                     KAKRLFLDISEGGVLLTSDVSESGEAKEIVPCQYEGPDCKISLNYTYLLTPLRVMEGE
                     YFSINFTEPNRSMTVKPESGRDYMHIIMPMQLNP"
     gene            6358..7446
                     /gene="recF"
                     /locus_tag="PKN79_00105"
     CDS             6358..7446
                     /gene="recF"
                     /locus_tag="PKN79_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605702.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNZ94206.1"
                     /translation="MRFCTITTNQFRNLKEKPLEVDASKVLLIGPNGQGKTNLLEALY
                     TLCYGSSFRTAQIKELATYGFKDFRIEGTYRDERNLLHTLTLTFKDNKRVIILDGTVI
                     RDRKELIYNIPTIVFSHDDIFFVKGEPEYRRKFFDQTMSMYNPLFFDDHRRYRQVLRQ
                     RNQAIKDEQYSLLPIYDQQLARHGIAIQRERVRSVYEFLQIFPEIYQEVSRSDTLLDI
                     EYRPSWEECATEEEVVHCLQSHRRRDIKLQTTTSGIHRDQFMITEDGYPFANKGSTGQ
                     LRLASLALRVAQMAYFTKKTDKEAVILVDDVLLELDIPKRERFLTILSGYSQAFFTFL
                     PEERYIHSFERSDRKVYHVSEGAFTLGQ"
     gene            7418..7759
                     /locus_tag="PKN79_00110"
     CDS             7418..7759
                     /locus_tag="PKN79_00110"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNZ94207.1"
                     /translation="MKEHLLLASDKKPRLSKSDDPLHGKDVLDIVLKTLDVDLGEPRY
                     GLAASWAEIVGPELYLHVKVVDIQHESLVLRADHPSWANLVLLQKKRIIKAIQNRYPS
                     LGITTLHVLGA"
     gene            7858..8025
                     /gene="rpmH"
                     /locus_tag="PKN79_00115"
     CDS             7858..8025
                     /gene="rpmH"
                     /locus_tag="PKN79_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNZ94208.1"
                     /translation="MSYKGKRTYQPSRVKRNRKFGFRARMATKGGRLVLARRRAKGRY
                     KLSVADEKKPF"
     gene            8006..8356
                     /gene="rnpA"
                     /locus_tag="PKN79_00120"
     CDS             8006..8356
                     /gene="rnpA"
                     /locus_tag="PKN79_00120"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNZ94209.1"
                     /translation="MRKSLSKSEIVKRRSEIDRIFKTGKKISSRGMNLIVTTNDLPFS
                     RIVVIPVRGYGSSVERNRIRRQIKEIWRTEKDRFLPGYDFAFVVYPGRNVDHALQRRH
                     VLTLCKQAGVMNPP"
     gene            8413..10194
                     /gene="yidC"
                     /locus_tag="PKN79_00125"
     CDS             8413..10194
                     /gene="yidC"
                     /locus_tag="PKN79_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNZ94210.1"
                     /translation="MDRNTILAVILSVLVITVGMTIQATFFAPSAQEEVAEEAANVEE
                     IYLQPEAYNKLPSNLAWGTDLPGSLIALESPASSSPFVYETDIFLITFDPKGASISSF
                     KLKKHLDKGEPVELLFGGSNANNAFLMYAGSDRTQPIDAPFNYRIAGNQVIFTQTFAI
                     KGEDVPFTLTKTFTFGESEYLFQIDVNLVNSENKAIPLSFDNTAYTIGFEPQIGPSFT
                     SIDNRYSYRRFYIREDGAKKKATITVKNGIYTTEKPLSWVALTGKYFSMIAIPDATNY
                     TVTLSEDTKTDIPLESNVFFSRPAIRSADQTDTYRFYIGPQLKADMVPYNDRASNSFG
                     LSNLFLEDTLDSSSWLGWLETILKTILNLLYKVIPNYGVGIIILTFLLKLILHPITKK
                     GMESTAKMSALGPQMEEIKARYPDNPTKQNEEMAELYKREKINPMGGCLPMLLQFPIL
                     IAFYGLLNKHFELRGAMFIPGWIPDLSVPETVATLPFNLPFLGNEIHLLPILYTVSMI
                     YSMKITQNSSGGAASQQGMMKMMTYGMPIMFFFVLYNAPSGLILYWSVMNVISVVQQL
                     YTNKKKQKEGEQEKVIQFQPKAKGKKR"
     gene            10222..10893
                     /gene="jag"
                     /locus_tag="PKN79_00130"
     CDS             10222..10893
                     /gene="jag"
                     /locus_tag="PKN79_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNZ94211.1"
                     /translation="MIREFEGRTEQEAIAKAIEELNIEREDFDVEIIEPARKGLFKKN
                     SVKIRIHFDDGMDEEESYYDEGEDLDEPFDSDLEDKVLTFVATVLEKMGYAGNVSIAF
                     RKERKIGLRIDSENSNIIIGRKGKNLDALQLLTNVFVGQQNSNVRVIVDSENYRIRHE
                     EQLIRLAYRTAEQVARSGQSRLLEPMNPYERRLVHTALNDFGGVETKSEGDGLYKQVR
                     IIATN"
     regulatory      10917..11119
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         2747 a         2948 c         2848 g         2604 t
ORIGIN      
        1 ctttccttca tcggacagca gctcgttgta gtaggcgatc ttcgcctcca gatcttcaag
       61 ctcttcaaga atcttggatg attcaaggtg gctgagccgg ccgaggcgca tgtcgatgat
      121 cgcctgggct tgagtttcat caagggcgaa ggcggcgatc agccgctggc tcgcgatgct
      181 gttgtcaggc gaatctttga tgatttgaat cacttcatcg atatgttcaa gaccgatctt
      241 aagcccgcgg aggatatgag cgcgctcctg agctttgcga agatcgtagg tcgtccgcct
      301 cgttacgaca tctttgcgat gcttcacata gtagtggagc atatccttca atccaaggag
      361 ttggggcctt ccatcgacca aggcgagatt gttgacgttg aagttcgatt gcagagcggt
      421 ccgctggaag agctggttca agacgaccat cacttcagcg cccatcttca gttcgatgac
      481 gatccgaatc ccgttgcggt cggattcatc ccgaatcgtg ctgatcatgg gaatcgagcc
      541 atctttccga agatcgtcga tcttcttcac cagatccgct ttgttcacct gataggggat
      601 ttccgtgaag acaatgacat cgcctccgcg ggcggactct tcgatctcgt acttccctcg
      661 ggtgatgatt cgtccccgac ccgttgaaaa cgcgtccacg atccccctca tcccgcaaat
      721 cacccctcca gacggaaagt ccggcccctt gatgtgctcc atgagctcca tcaacgttat
      781 ctcgggatct tcaatcaacg cattggcagc ggcacagatc tcctgaaggt tgtgcggagc
      841 catgttcgtc gccatgccga ctgcgattcc actcgacccg ttggccagaa ggaagggaaa
      901 cgcaccggga aggacagagg gctcggtcag cgaatcgtca tagttcggct tgaagtcgac
      961 ggtttctttt tgaatgtcgc tgagcatctc ttcgccgatg cggctcatct tcgcttcggt
     1021 ataccgcatc gccgccgcgg gatctccgtc gatggatccg aagttgccct gggggttcac
     1081 caccggatac cgaagcgaga aatcctgagc gagccggacc agagcgtcat acacggaagc
     1141 atcaccgtgg gggtgatact taccgagaac gtctccgacg atgcgggcgc ttttcttata
     1201 ggaggagttg gccctgaggc ccatctcata catatcatag agaatgcgcc gatgaaccgg
     1261 cttgaggcca tcgcgcacat cgggcagggc ccgactgacg atcacgctca tcgcgtagtt
     1321 gagatagctc gtgcgcatct cgcttgcgac atctacttta atcgtcctgt tttccatcgt
     1381 atccacaatt ctatccttgt ttacacatca agattggaga catacaccgc attcgcatcg
     1441 atgaacgccc ggcggggctc gacctcttcg cccatcaaca tcgagaacac ccggtccgct
     1501 tcctcggcat cgccgagggt cacctggctg atcaggcggg tctcgggatt catcgtcgtc
     1561 tcccacagct gctcggggtt catctctcca aggcccttgt agcgctggat ggaggccttt
     1621 gccgcatcag gaccggcttc ttcaagaatc ctgatccgag cttcttcatc atacgcgtag
     1681 gaaacatttt tcccgatcgt gatcttatac agcggcggca tcgcaaaata gacatatccc
     1741 gcttcgatca acggacgcat atagcggaag aagaacgtca agagaagcgt tcggatatgc
     1801 gagccgtcga catcggcgtc cgccatgatg atgactttat gatatcgggc tttggtgatg
     1861 tcgaacgtta catcgctgtc gagatcctcg tcttttccca tgttgttgta gcgggtgagc
     1921 cccgctccga tcgtgctgat caccggctga agcttgtcgt tgcccaaaac tcggtattct
     1981 tgagcttttt ccacgttcag catcttgccc cacagcggca agatcgcctg gaagcgccga
     2041 tccctgcctt gcttggcgga tcctccggcg gagtctccct cgacgatgaa gacttcacag
     2101 agcgccggat ccttctcgga gcagtccgcc agcttgccgg ggagacctcc tccttcgctt
     2161 ttctttcgga tctgttcccg cgcttttcga gcggcgatcc gtccgagcgc cgcgttgatg
     2221 atcttcccca agatcagttc ggcgtgggcc ggaaactcgt caaagtagtt gccgagcttt
     2281 tcatagacca gggaaaagac cacgccggta acgacgctgt tgcccagctt catcttcgtc
     2341 tgaccttcaa attgaggctc gggaatcttg atcgacacga tcgccgtgag cccttcggag
     2401 atatcgctcg gctcaaggcg gtcctcatac cgcttcagaa gcttctggtt cttctgcagc
     2461 tgggcgttga tcacccggga aagcccggtt cgaaagccgg tcaggtgcgt tccgccctct
     2521 ttcgtgttga tattgttcac gaaggtcaga accttctcat cgtatttgtc gttgtattgg
     2581 agggcgatct caactttgat gttgtcccgc tccccctcga tggagatcgg cgtttcgccg
     2641 agggtgcgct tgtattcatt aagatagcta acaaactcaa cgataccccc ttccgagtga
     2701 aaggtttgct ttttcggctc ttcaaagcgg gtatcggata ccgttatggt aattcctttg
     2761 tttaaaaagg agagttcccg gaaccgggtt acaagggttt catagctgaa tgcattctct
     2821 tccaggatcg tatagtccgg tgaaaacgta accttcgttc cggtctcgtc ggtatcgccg
     2881 atccgctgaa cctcactggt gggattgcct tggctgtaga gctggcggaa gattccgcca
     2941 tcccgcttca cgatcacttc catccagatg ctgagggcgt tcacacatga aacaccgacg
     3001 ccatgaagcc ctccgctgac cttgtaggag ttcttgtcga acttccctcc ggcgtggagc
     3061 tgcgtcatcg cgatctcaag cgaactcttc ttttcaatcg ggtggatggc gacggggatt
     3121 ccccgtccat catcttcgac ggtacagaac tgccgtcctt cttcatctcg ggaaagtgtg
     3181 acggtaatcg tgtcgcaata gcccgccatc gcttcatcga tcgagttgtc cacgacctcg
     3241 tagacgaggt ggtgaagtcc gtcaattccc gttgaaccga tatacatccc cggtcgtttt
     3301 ctgacagctt caagcccttt gaggacttga atcgaggaag cggaatactg atccgggggc
     3361 ttcactgcac ccgttgcttt tgcacccgcg gaaaggtcag cattcccatt ggaaaaactc
     3421 ccatcattca ttggtatatc cttatgtaat aataaaaaaa tctataaatt ccattcgata
     3481 aattgtatca gaaatggcaa agtgttgcaa gcggaccccg cagtttttca caaaatatat
     3541 atactttcaa gataatgatt tatgctcaca tgaaactgct catatggaga atgcttgcac
     3601 cgtcgttcac tcaacggtag aataggtggt atgagcgacc aacaaacatc atattatgag
     3661 ttctggctgg agacggcaaa gaggattcag gaaacccttc cgcctcagga attttccact
     3721 tggttcagcc gaatctccta tctctcctcc aaaaaaggag aggtcaccct cgcaggtccc
     3781 agccaattcg tcctcgatac cgtaacgacc cgttaccgat cgatcatcgc gaacaccctc
     3841 agtgaactta ccggtgagga gatcgccgtc gagtttgtcg tctccgacaa aggaaaaaaa
     3901 gccgtccggc cgacggacgg cgatgaaacc gctgaagaag atcggaaaga gaaaaaagcc
     3961 gatgatcttg ccaagaagat ttccaagcgg aaaagcgagg cgaatctgaa tccctcctat
     4021 gaattcgaat ccttcgtgct tggcgacaac tccgcctttg cgtacaacgc gagtctggcg
     4081 atcgcaaaaa atccgggttc gagctacaac ccctgtttga tctatggcga tgtgggcctc
     4141 gggaagaccc atctcctcag ctcgatcgga aactatatcc tcgatcatac ccccgaatgc
     4201 aaggtgatgt acgtcaccgc cgaaacattc accaatgaat tcatcgagtc gatcggaagc
     4261 cagaaaaccc agcagttcaa gaacaagttc agaaacgtcg atgttctgtt gatcgatgac
     4321 atccactttc ttcaggggaa ggattccacc caggaagagc tcttccacac cttcaacaac
     4381 ctctatgaag cgaagaagca gatggtgttc acctgcgacc gcccgatcag cgaattgaag
     4441 aacatcaccg atcgcctttc ctcccggttc gagcggggat tgaatgtcga tcttcaaccg
     4501 ccaaactatg aaacgagggt tgcaatcctt aagaagaagc ttcaagaaca tggttcatcc
     4561 ctctcagagg atatcatcaa ctatatcgcc ttcaatgtcg taaccaacgt tcgagacctt
     4621 gaatcctcgc tgacgaagct tgtcgcctac atggacctga tcggggaaga gttgacgttc
     4681 gagaaagcaa agcagcttct ggggattctt cccgcgatgg ccaacatcgc ccgggacact
     4741 ctatccatcg gcgagatcat caagaccgtc ggtgactatt tcaacgtctc gacgttcgag
     4801 atgaaaggga agaagaaaaa caagagcctg gtccagccaa ggcagatagc gatgtacctc
     4861 gcccggcaaa tcaccgactt ttcaacaacg gagatcggga tggaattcgg cggccgagat
     4921 cataccacgg tgatgtatgc gaacaaccgg gtcgaatcgc tgatgaaagg agacgaggcg
     4981 tacgcggcga ccgtcaataa actgcgtcgt caattgacct cttcctgagc tgtatattct
     5041 ggggataacc tcttgggaaa atgtggacaa cgcttcaagc ctgtggatag cttattattg
     5101 cttgtggatg aactgtggat atcttttgaa cctattcaca agctggaaaa atttaatagg
     5161 ttcttttaca atgaattgtc tcagttatca acagtttctg tgctattatt actattatta
     5221 ctgattaata ataaaatagg agtagagtaa gatgaagttc atctcaacaa aagaagcgat
     5281 tctttcagaa atcatataca cggtggattt caccactcag aagaatagtc ttgcgattac
     5341 cagcaacgta taccttcaaa ctctcggcga tcagctgttg attaaagcca ccgacggcaa
     5401 agtgggattt tccacttcaa tcaccgtcga tgttcaggaa gaaggaaaag ctcttgttct
     5461 tcaggaaaaa ttgttggaga ttcttcgagt tctaccggat ggagatatca acttctcgat
     5521 gaaggacggc ttcttcacga tcgtacccgt cggtcatggg attgatttca agatccgaat
     5581 gattgaagcc gatcagtttc cggctcttga aacgattgac aatgatcgat tcttcagcat
     5641 cgcccaaagc tctttccttg agatgagtga acaggtcatt ttcgccgtca gcgacgatac
     5701 atcccgatac tttctttgcg gagtctatct tgagcgaagc gaaggcggaa taacgatggt
     5761 ggcgaccgac gggagaagac tttcgatcgt cgagcgggtg ttcgaagaaa aactgccctc
     5821 cttcgcaccg atcatcgtgc tgccgaagtt tttccagatt ctccgcaaga tcggcaccgg
     5881 tgagggggcg gtcgagcttg caatcaccga gtcaattctc tttgcccgaa ttggaggacg
     5941 gacgttctat atcacgttga tcagggagca gtatccgaac ttccgccggg tcatccccga
     6001 gcagcagagc taccggtgca cgatgaaaat tcaggatatg gaagatgcgc tgaggcgggt
     6061 ggcgatcctt atcgagtcaa aggcgaaacg cttgttcctg gatatcagtg aaggcggcgt
     6121 ccttctgacg agcgatgtca gtgaaagcgg agaagccaag gagatcgttc cctgccagta
     6181 tgaaggaccg gattgcaaga tctctttgaa ctacacctat cttctcactc cgctcagggt
     6241 gatggagggg gaatacttct cgatcaactt caccgagccg aaccgatcga tgacggtgaa
     6301 gccggaaagc gggcgtgact acatgcatat catcatgccg atgcagctta atccttgatg
     6361 cgcttttgta cgattacgac gaatcaattc agaaatctca aggagaaacc cttggaagtg
     6421 gacgcttcca aggttctcct tatcggcccc aacggccaag gcaagacgaa tctgcttgag
     6481 gcgctgtaca cgctgtgtta cggctcttcc tttcgaacgg cccagatcaa ggagctggca
     6541 acgtatggct tcaaggattt tcgaatcgaa ggaacgtatc gggatgaacg gaatctcctt
     6601 cataccctca ccctcacgtt caaggacaat aaacgggtca tcatcttgga cggtacggta
     6661 atccgtgacc ggaaagaact gatctacaac attccgacga tcgtcttcag ccacgatgac
     6721 atcttttttg tcaaagggga gccggaatat cgacggaagt tttttgatca gacgatgagc
     6781 atgtacaatc cgctcttttt cgatgatcac cgccggtatc gtcaggttct gcgccaacga
     6841 aaccaggcga tcaaggatga gcagtactcc ttgctgccga tctacgatca acagcttgcc
     6901 cgccacggaa tagccattca acgggaacgg gttcgttccg tctacgagtt tctacaaatc
     6961 tttccggaaa tctatcagga ggtctcccgg agcgacaccc ttctggatat cgagtatcgg
     7021 ccatcctggg aggagtgcgc caccgaggag gaagtcgttc actgcctcca atctcatcgt
     7081 cggagggata tcaagcttca aacgacgaca agcgggatcc atcgtgacca attcatgatc
     7141 accgaggatg gctacccgtt cgccaacaaa gggtcgacgg gccagctgcg cttggcttcg
     7201 ctggcgctcc gggtcgccca gatggcgtac ttcacgaaaa aaacggacaa agaggcggtc
     7261 atccttgtcg atgatgttct tttggagttg gatattccaa agcgcgagcg gtttctgacc
     7321 attctttccg gctatagtca ggcgtttttc acgtttctac ctgaagaacg gtatatccac
     7381 tcctttgaaa gatccgatcg gaaagtgtat catgtcagtg aaggagcatt tactcttggc
     7441 cagtgataaa aaaccccgcc tcagcaaaag cgatgatccc ctgcatggta aagatgttct
     7501 ggatatcgtc ctcaaaacgc ttgatgtcga tcttggagag ccgagatacg gtcttgctgc
     7561 aagttgggcg gagatcgtcg gacctgagct gtatctccat gtgaaagtgg tggatattca
     7621 gcatgaatcg ctggttcttc gagcggatca tccctcgtgg gccaaccttg tgctgctgca
     7681 aaaaaaacgg atcatcaagg cgattcaaaa ccgttatccg agcctcggaa tcacaaccct
     7741 tcatgtgctt ggcgcttgac ggaaaacagt gctctctata tactgcacca ctcgggggga
     7801 gtgtcccccg cttaatatga ataatccgcg ttaagcgatg taaattggag aattcccatg
     7861 agttacaaag gtaagagaac ctaccaaccc agccgggtta aaagaaacag aaagttcggg
     7921 ttccgcgctc gtatggccac gaagggagga aggctcgtcc tcgctcgccg ccgggcaaag
     7981 ggtcgctaca agctttctgt tgcagatgag aaaaagcctt tctaagagtg aaattgtaaa
     8041 acgtagatcg gaaatagatc gaatctttaa aaccgggaag aaaatttcaa gccggggcat
     8101 gaacttgatt gtgacgacca atgaccttcc gttcagccgg atcgtggtta ttcccgtccg
     8161 aggctatggc tcatcggtgg agcgcaacag aatccgtcga cagatcaaag agatctggcg
     8221 tactgaaaaa gatcgatttc ttccgggcta cgattttgca tttgtcgtat acccaggaag
     8281 aaatgtagac catgctcttc aaaggcgaca tgtactcact ctgtgcaagc aagccggggt
     8341 gatgaatcct ccctgatttc tttgagtcta cctattcctg aacagcacct tcttccgata
     8401 gcaggagtag tcatggaccg aaacaccata ttagcagtca ttctcagcgt gctcgtcatc
     8461 accgtgggga tgacgattca agcgacattt tttgccccga gtgcgcaaga agaggttgcc
     8521 gaagaggctg ccaacgtcga agagatctat cttcaaccgg aggcctacaa caaacttccg
     8581 agcaatttgg cgtggggaac cgatctgccg ggttcgttga tcgctcttga aagtcccgcc
     8641 agctcctccc cgtttgtcta cgaaacggat atctttttga tcaccttcga tcccaaaggc
     8701 gcttcaattt catccttcaa gctgaagaag cacctggaca agggcgagcc ggtggagctg
     8761 ctcttcggcg gttcgaatgc caacaacgcc ttcctcatgt atgccggatc cgaccgtact
     8821 caaccgattg acgctccgtt caactatcgg atcgccggta accaagtgat cttcacccag
     8881 acgttcgcga tcaagggtga ggatgttcca ttcacgttga caaagacgtt caccttcgga
     8941 gagagcgagt atcttttcca aatcgatgtg aacctcgtca acagcgaaaa caaggcgatt
     9001 cccctttcct tcgacaatac ggcgtatacg atcggctttg agccgcagat cgggccttcc
     9061 ttcaccagca tagacaaccg ctactcctac cgccgcttct acatcagaga ggacggagcg
     9121 aagaaaaaag ccacgatcac ggtgaagaac gggatctaca ccacggaaaa acccctttcc
     9181 tgggtcgcct tgaccggcaa gtatttctcg atgatcgcga ttcccgatgc gaccaattat
     9241 accgttacgc tgagcgaaga cacgaaaacc gatattcccc tcgagtcgaa cgtgttcttc
     9301 agccggccgg cgattcgaag cgccgaccag acggatacct atcgcttcta catcggaccc
     9361 cagctgaaag cggacatggt tccctacaac gatcgggcga gcaacagctt cgggctttcc
     9421 aacctgttcc tggaggatac cctcgattcc tcttcgtggc tggggtggct tgaaacgatt
     9481 ctgaagacga ttctcaacct tctctacaaa gtgattccca actatggggt gggaatcatc
     9541 attctgacct tcctccttaa gctgattctc cacccgatca ccaagaaggg gatggagtcc
     9601 accgccaaga tgagcgccct cgggcctcag atggaggaga tcaaggcgcg ctatccggat
     9661 aaccccacca agcagaacga ggagatggcg gagctctaca agcgggagaa aatcaacccg
     9721 atgggcggct gtctgccgat gctcctccaa ttcccgattc tcatcgcatt ctacggattg
     9781 ctcaacaaac actttgagct tcggggagcg atgttcatcc ccggctggat tcccgacctc
     9841 tccgttcccg agacggtggc gaccttgccg ttcaatttgc cgttcctcgg gaatgaaatc
     9901 cacctgctgc cgattctcta taccgtgagc atgatctact cgatgaaaat cacccaaaac
     9961 tcttccggcg gagcggccag tcaacaggga atgatgaaga tgatgaccta cggcatgccg
    10021 atcatgttct tcttcgttct ctacaatgct ccctccggct tgattctcta ctggtcggtg
    10081 atgaacgtga tctcggtcgt tcagcagctt tacaccaaca agaagaagca aaaagaagga
    10141 gagcaggaga aggtcatcca attccagccc aaggcgaagg gaaagaaacg ataaaagatc
    10201 aacaccgaag aggtaccata tatgatacga gaattcgaag gacgaacgga acaggaagcg
    10261 attgcgaaag cgatcgagga actgaacatc gagcgggaag attttgatgt cgaaatcatc
    10321 gagcctgctc gcaaagggct gttcaagaag aacagcgtga aaataagaat tcactttgat
    10381 gacggaatgg atgaagaaga gtcctactat gacgagggcg aggatctgga cgagccgttt
    10441 gacagcgatc tcgaggacaa ggttctcacg ttcgtggcga cggtacttga gaagatgggc
    10501 tatgccggca acgtttcgat cgccttcagg aaggagcgga agatcggctt gaggatcgac
    10561 agcgagaact ccaacatcat catcggccga aagggcaaga accttgatgc gctccaactg
    10621 ctgacgaacg tcttcgtcgg acagcagaac tcgaacgtcc gagtgattgt cgacagtgaa
    10681 aactatcgga tccgccacga ggagcagctg atccgccttg cgtaccgcac cgccgaacag
    10741 gtcgcccgaa gcggccaaag ccgccttctt gagccgatga atccctatga acggcgtctg
    10801 gttcacaccg ccctgaacga cttcggcggc gtggagacca agagcgaggg ggacggcctc
    10861 tacaaacagg tgcgaatcat cgctacgaac tgacttgacg cttgacaagc gtaccgggtg
    10921 aatctaccat gagtggtgta tcgcatgata cactacacta tatgttgagt gtataggaaa
    10981 agggaagttg ggtgaaacgc ccacgcggtc ccgccgctgt gatggggagc gattggagat
    11041 gaccactgga gtgatccggg aaggttccaa gagctgcgat cctgagccag aagaccttgc
    11101 ctatatggtc acgccgccac ggagcatggc aggagcgtga gagccga
//
LOCUS       DAOPUZ010000003         8727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_8065_length_8727_cov_3.576684, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000003 DAOPUZ010000000
VERSION     DAOPUZ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8065_length_8727_cov_3.576684"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKN79_00135"
     CDS             <1..219
                     /locus_tag="PKN79_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ94212.1"
                     /translation="QALLQFFSQLATVVILFVTTIIKERYQLVLPAILATILLGTLIA
                     TFFIKESEVIVTEDERLKALIELENVQH"
     gene            complement(221..793)
                     /locus_tag="PKN79_00140"
     CDS             complement(221..793)
                     /locus_tag="PKN79_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ94213.1"
                     /translation="MPVLSTFQNRRAYRALDSKPIDEEVLTRLVEAAITAPSANNSQP
                     WRFITATDPAVLAEVKNTLTPGNYWAQKAPAITAVVTNNDWSLVIGDRIFAPFEAGMA
                     AMAYQAQAVSEGLHIHPIAGFNADAVKEALKIPASEMLLTLLIIGYPGDDSELREHHL
                     KSERSPRVRLPLDHVYAKDRWNDRLLPQKP"
     gene            complement(897..1058)
                     /locus_tag="PKN79_00145"
     CDS             complement(897..1058)
                     /locus_tag="PKN79_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNZ94214.1"
                     /translation="MQQYECNLCGYIYDPAVGDPDNGVAAGTAFADIPEDWVCPLCGA
                     PKSDFSPLD"
     gene            complement(1118..2083)
                     /locus_tag="PKN79_00150"
     CDS             complement(1118..2083)
                     /locus_tag="PKN79_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HNZ94215.1"
                     /translation="MKGERVAQIELLLHSHPEGLRRAEIARRLGVHRSTISRYIDKLG
                     EYIDLYEENSLLKIRAKEGEEVHALSVYESLAINLSAEMLLNTGEFQNPHLASGLRKI
                     AMNMRSYAPKISENIVSLAEQIDKQLIAQKESSAFNAILEVLIDSWVSGRIVRIVQSL
                     KGFDSIETELAPYFIGFRETDGTRHPITVTGRLRHTTEIVTVDISTITSATILDETYT
                     IPDNLKPFKFPDAPETFDAIDMIPLSLKLKERSAMNAFRQLVHGKPQLEKLKDGSWIC
                     NMDVENSIELTLRIIQCGDAVEILGPEHYRRRFMKRLEDVLAIYR"
     gene            2280..3428
                     /locus_tag="PKN79_00155"
     CDS             2280..3428
                     /locus_tag="PKN79_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNZ94216.1"
                     /translation="MIDYLFHEESYSKSVFNEYRSVSPGDGGLLVGGLVFGEQLAEFA
                     RLPFDALLRHLIPHPEKGIENIGGPAAVSLIHAAQLLHDSPHSFHFYGVAGDDVEYRL
                     IREHIAKTVIDPRFLILEGERTPTTYVLNDRHAHGGKGERTFVNLLGSAAHVIEADVG
                     DDAYQSDIVLFGGTALWPAFHTRIDQALRRAKAEGALTIVGAVFDFMNEHRSSSEKWP
                     FGQESGYPFVDLLVCDALEARRMSGQRDAENAAAFFDEAGVGAGIITQGAKDIIAWSK
                     EESVFGHTPLTRFPVCGYIDELLEKDPSLRKDTTGCGDNFLGGVLASIVSHGAKKCSM
                     VNAITLGGASGGFALTHHGGMFHETYPGEKRSLLEPVLKAYSEQLGVI"
     gene            3436..3630
                     /locus_tag="PKN79_00160"
     CDS             3436..3630
                     /locus_tag="PKN79_00160"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNZ94217.1"
                     /translation="MDDRLINVEMQLTFAQEKLETLNGVVTEQGMELQRLKTLVEKLE
                     RRIVDLVEGSEGGPQKPPHY"
     gene            complement(3627..5300)
                     /locus_tag="PKN79_00165"
     CDS             complement(3627..5300)
                     /locus_tag="PKN79_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ94218.1"
                     /translation="MATYLIVGGVAGGAGVAARLRRRDENAQIIIFERGEHISFANCG
                     LPYYAGGVIENRSSLFVMTPELFKRVLNVEARVNSEVVSVDRVQKTITVRNVKDDTTY
                     PQAYDHLILSPGATPIRPRIPGIDHPSIMTLRSVSDIDRIKTAVDRSETKRAVVIGGG
                     FIGLEMAENLKHRGLEVSLVEALEQVMGVIDYDFAAQVQQHMRKKGVTLHLKDPVKGF
                     SKNGEAVLVELESGKVLETDLVILSIGVRPDTAFLKESGITLSSTGAIVVDEYFTTND
                     PFIRALGDAICFACPLTDHSITVPLAGPANKQARLLADALIDGNRKPYRGTIGTSIAK
                     VFDLTVASTGLAEKTLIALKLPHKSAVTHTSSHADYYPGSLSLSLKILFHPTTGKIWG
                     AQAIGYEGVDKRIDVISSFIGMDGTVYDLAEFEHAYAPPFSSAKDPINMVGFIGMNAL
                     EGISNPISWREVDNLIKQGAVILDTRSEMETMTGGMLPGSINIPHTALRKRIAEVPKD
                     KPIITTCVMGVRGYLAERILRGNGFTKVYNLTGGLHSYTTAMSERARLN"
     gene            complement(5285..5650)
                     /locus_tag="PKN79_00170"
     CDS             complement(5285..5650)
                     /locus_tag="PKN79_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ94219.1"
                     /translation="MFNLCAIRRLQSALRSFEEQLKDQTGLSFNDALLLCAVEKGIVE
                     PSALAKELDLSPSRLTRVIDSLEGRSLVQRTLSITDRRSLIISLTETGEELVRTYKCC
                     ELRLPDELAFTQEESWQHI"
     gene            6114..6725
                     /locus_tag="PKN79_00175"
     CDS             6114..6725
                     /locus_tag="PKN79_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ94220.1"
                     /translation="MKTILCYGDSNTHGSNPGGGRWEYDKRWPGILSSLLGDGYYVIE
                     EGLGGRTTVFDDPFEKGRNGKTYLPVVLASHQPIDLLIISLGTNDTKRFFGSNERIIT
                     RGIEALADVAKTFDVKQIMVISPIHVGARIEETIFAGFSKESVEISYKLSERVKAMAE
                     RKGLIFFDAATAATPSEIDQLHMDAESHRSLAEALKEVIIQHL"
     gene            complement(6746..>8727)
                     /locus_tag="PKN79_00180"
     CDS             complement(6746..>8727)
                     /locus_tag="PKN79_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94221.1"
                     /translation="TLTYEDKHQHPIALEIEDDMVFVSFEGIGTIEDRLAPGRNGVTV
                     DRTWILVKEGSYRLQAGFFTTDEGVPNDLFIPAVWYRDNTKGSGLFPSIDHSSTWSFL
                     ETRMSIPCCLQLSGGKRHFLCATEAAAEERFVASVTNDPHGAIISIPGWEGPYSYQGK
                     KALIDTTSMPRPALMVPSGGLIYRRTFHLATYRGMRGMEVYVAFVASLPKAKPTDLPH
                     PLSWNHWMELKLTRLINLVRRSEGNLAYIIMGEGNGEVQDVYEFTGASFLVKSIQGAC
                     ELARFTEHEPTLDCLQAARRALAKRFSLVDDSRLLVTVAKKIGDFFLKAERREGVFQD
                     NHDLRTGQWGGYLGIGEFPEFAEMVNSRCNGEAMKHYLRLAKELRQLGYDVSPYIDLA
                     RRVALFYSEIQLSSGSFGRWWTPEGKPKDIQGTNGAYIGSFLCALIPMLEESDPLKAV
                     LIDAVHKAYMFYARLADEGAFYGDTLDADSCDKEAGVALLSFFMDLYHLDGNKRHYLK
                     SAHLAAEFIIQWIWQQSSYLPQESPLGKKGFSTLGMTSVSVAHHHLDCYGMAIAYEFL
                     RFATAANLPFYAEQASLMIAACKQLVHGKENDLGRDESFFGWQPEQINHTNWEYFNRP
                     ELMNGHYEIDIAWVTILTLSFFDRIRGEFPEALQE"
BASE COUNT         1961 a         2262 c         2419 g         2085 t
ORIGIN      
        1 caggcgcttc tccagttttt cagccagctc gccaccgtcg tcatcctctt cgttacgacg
       61 atcatcaagg agcggtatca attggtcttg ccggccattc ttgcgacgat tctgctgggt
      121 accctgatcg cgacgttttt catcaaggag agcgaggtga tcgtcaccga agatgagcgc
      181 ctcaaggcgc tcatcgagct ggaaaacgtt cagcattaag ctatggtttc tgtggaagga
      241 gtcggtcgtt ccaccgatcc tttgcataca catggtccaa aggcaggcgc acccgcgggc
      301 tgcgttcgct tttgagatgg tgctccctga gctcggaatc atctccgggg tatccgatga
      361 tcaagagggt cagaagcatt tccgaagcgg ggatcttcaa agcctctttt accgcatcag
      421 cgttgaagcc ggcgatcggg tggatgtgca acccttcgga taccgcttgt gcctgatagg
      481 ccatcgcggc cattcccgcc tcaaaggggg caaatatccg atccccgatg acaagcgacc
      541 agtcgttgtt ggttacgacg gcggtaatcg ccggggcttt ttgagcccaa taattacccg
      601 gggtgagggt gttcttcact tctgcaagaa cggcagggtc ggtggcggtg ataaaacgcc
      661 atggttggct gttgtttgcc gagggagcgg tgatcgccgc ttcgacgagg cgggtaagaa
      721 cttcttcatc aataggtttt gaatcgaggg cgcgataggc gcgtcgattt tgaaatgttg
      781 atagtacagg cataattcct ccgtaatgaa atgaatatac tcaagactga acgaatcgtc
      841 tgtatacaga gacagggtcc ggaattccga accctgctat gtgcatcttg aattgcttaa
      901 tcgagtggcg agaagtctga cttcggcgcg ccacagaggg ggcagaccca gtcctccgga
      961 atatcggcaa aagcagttcc tgccgctaca ccattatcgg gatcgccaac ggccgggtca
     1021 taaatatacc cgcaaagatt acattcatat tgttgcatct catttcctcc tgcaccctag
     1081 cataccgata aagtatgttt cccgcaagaa aattcgtcta tcgatagatc gccagcacat
     1141 cctcaagacg tttcatgaaa cgccgtcgat agtgttcggg ccccagaatt tccaccgcat
     1201 cgccgcattg gatgatccgc aaggtgagct cgatcgagtt ttcgacgtcc atgttgcaaa
     1261 tccacgaacc gtctttgagt ttttccaatt gcggctttcc gtgaaccaac tgcctgaatg
     1321 cgttcattgc cgatcgttcc ttcagcttga ggctgagggg aatcatatcg atcgcatcaa
     1381 aggtctcggg agcatcggga aatttgaagg gtttgaggtt gtcggggatc gtgtaggttt
     1441 catccaggat cgtggcgctg gtgatcgtgg agatatcgac ggttacgatc tcggttgtgt
     1501 gtctcagtcg tccggttacg gtaatcgggt gacgtgttcc atcggtctcc ctgaagccga
     1561 tgaaataggg ggcaagctcg gtttcaatcg aatcaaatcc tttgagtgat tgaacgatcc
     1621 ggacgatccg cccgctgacc caggaatcga taaggacctc taagatcgca ttgaacgcac
     1681 tgctctcttt ctgggcgatc aattgcttgt cgatctgctc ggcgagcgaa acgatgtttt
     1741 cactgatctt tggagcatag gagcgcatgt tcatcgcgat ttttctgagc cctgaagcaa
     1801 gatggggatt ttggaactcg ccggtattca agagcatctc agccgagagg ttgatcgcaa
     1861 ggctctcata gacggagagg gcgtgtacct cttcgccttc cttcgcccga attttcaaca
     1921 ggctgttttc ttcatagaga tcgatgtact cgccgagctt gtcgatgtag cggctgattg
     1981 ttgagcggtg gactccgagt ctgcgggcga tttcagcccg gcggagtcct tcgggatggg
     2041 aatggagcag taattctatc tgtgcaacac gctcaccctt cataacaccc cctttgcatc
     2101 aaaatgcaac aaaacttgag atagtttacc acaggattgg aaagcggtaa acagaatgca
     2161 acataatgtt gtttaaggag aagatgcttg cgttaccgtt ggagtgtagt atcgtgaaag
     2221 gagcaccatc aatgatgata aggagtgatt gggtgagggt ttcagcaatc gggtgttgtt
     2281 tgatcgatta tctctttcat gaagaaagct actcaaaatc ggtgttcaac gagtatcgga
     2341 gcgtttcccc cggcgacgga ggattgctcg tcggcggcct agtgttcggt gaacagctcg
     2401 ccgagtttgc ccgcttgccg ttcgatgcgt tgctgcgaca cctgattcct cacccggaaa
     2461 aaggaattga aaacatcggc ggacctgcgg ccgtctctct tattcatgcc gcccaacttt
     2521 tgcatgactc gccgcattcg ttccactttt atggcgtcgc cggtgatgat gtcgaatacc
     2581 gattgatcag ggagcatatc gcaaagacag tgattgatcc ccgcttcctc atccttgagg
     2641 gggaacgtac cccgacgacc tatgtgctga atgatcgtca tgctcacggg ggaaaagggg
     2701 aacgcacctt cgtgaatctg ctcggttcgg ccgcccatgt cattgaggcg gatgtgggcg
     2761 atgatgcgta tcaaagcgac atcgtcctct tcggcggaac cgccctttgg ccggcgttcc
     2821 acacccggat cgatcaagcc ctccgccgag ccaaagcgga gggggcattg acgatcgtcg
     2881 gcgcggtgtt tgatttcatg aacgaacatc gctcatcaag cgagaaatgg ccgttcggac
     2941 aggaaagcgg gtatccgttt gtcgatctgt tggtgtgcga cgccttggaa gcccggcgga
     3001 tgagcggaca acgcgacgcg gagaacgccg cagccttttt tgacgaggcg ggagtgggtg
     3061 ccggaatcat cacccaggga gcaaaggata tcatcgcctg gtcaaaagag gagagtgtct
     3121 tcggccacac ccctttgacg cgcttcccgg tttgtggcta tatcgatgaa ctgttggaaa
     3181 aagatccttc gcttcgcaaa gacaccaccg gctgcggcga taatttcctc ggaggagtgc
     3241 tcgcctcgat cgtctcccac ggagcgaaaa aatgcagcat ggtcaatgca atcacccttg
     3301 gcggcgcttc ggggggattc gccctgaccc atcacggggg aatgttccat gaaacctatc
     3361 cgggtgaaaa acgaagcttg cttgaaccgg tgctcaaggc ctatagcgag cagctcggtg
     3421 tgatataatc atcttatgga tgatcgttta atcaatgtgg aaatgcagct taccttcgcc
     3481 caggaaaagc ttgagaccct caatggggtc gttacggaac aggggatgga attgcagcgg
     3541 ctgaaaaccc ttgtcgagaa gttggaacgc cggatcgtgg atctcgtgga aggaagtgaa
     3601 ggcggcccgc aaaagccgcc tcactattaa ttcaatcgag cgcgttcact catcgccgtg
     3661 gtgtaggagt gaagccctcc ggtgaggttg tacaccttcg taaagccgtt tccccgcaga
     3721 atccgttctg ccagatatcc ccgcaccccc atcacgcacg tggtgatgat cggcttgtcc
     3781 ttcggaactt cggcgattcg tttccttaaa gcggtgtgtg gaatgttgat cgagccgggt
     3841 agcattcctc ccgtcatcgt ttccatttcg cttcgagtgt cgaggatcac cgccccctgc
     3901 ttgatgagat tgtcgacttc tctccagctg atcgggttgg agatcccttc aagggcgttc
     3961 atcccgatga agccgaccat gttgatcggg tccttggcac tggagaaggg aggagcgtag
     4021 gcgtgttcga actcggcgag atcatacacc gttccatcca ttccgatgaa cgagctgatc
     4081 acgtcgatcc gtttgtcgac cccctcgtag ccgatcgcct gagcacccca gatcttcccg
     4141 gtggtcggat ggaacagaat cttcaagctt aaggagagac tgccggggta atagtcggcg
     4201 tgactggagg tatgggtaac cgcgctcttg tgggggagct tcaaagcgat gagggtcttt
     4261 tcggcaaggc ccgtcgaagc gacggtgaga tcgaagacct tggcgatcga tgtgccgatc
     4321 gttcccctgt agggcttcct gttcccatcg atgagggcat cggcaagcag gcgggcctgc
     4381 ttgttcgcag ggccggccag cggcacggta atcgaatggt cggtcaaggg acaggcgaag
     4441 cagatcgcgt ctcccagcgc ccgaataaag gggtcgttag tggtgaagta ttcatccacc
     4501 acgatcgcgc ctgttgaaga aagggtgatt ccgctttctt tcaggaaggc cgtatccggg
     4561 cggactccga tggaaaggat caccagatcg gtttccagca cctttccgct ttccaattcg
     4621 acaagcaccg cctcgccgtt cttgctgaag cctttgaccg gatccttgag atggagggtg
     4681 actccttttt ttctcatatg ttgttgaacc tgggcggcaa aatcgtagtc gatgacgccc
     4741 atcacctgtt ccaatgcttc gacaagggag acttccaaac cccggtgttt gaggttctcc
     4801 gccatctcga ggccgatgaa cccgccaccg attaccaccg cccgcttcgt ttcgctgcgg
     4861 tcgaccgccg ttttgatccg atcgatatcg ctgaccgaac gcaaggtcat gatcgaagga
     4921 tggtcaatgc cgggaatccg cgggcggatc ggggtcgctc ccggggagag gatcagatga
     4981 tcatacgctt gcgggtaggt ggtgtcatcc ttgacgttcc gaaccgtgat cgtcttctga
     5041 actctgtcga cgctcacgac ttcgctgttc acccgtgctt caacgttgag cacccgtttg
     5101 aagagttcgg gggtcatcac gaagagggat gaccggttct cgatgacacc gccggcatag
     5161 tagggcaggc cgcagttcgc aaagctgatg tgttcgcctc gttcaaagat gatgatctgt
     5221 gcgttctcgt ctcgccgtct gaggcgagcg gcgaccccgg ctccgccggc gactccgccg
     5281 acaatcaaat atgttgccat gattcctcct gggtaaatgc cagttcgtcg gggagccgga
     5341 gttcacagca cttataggta cgaacaagct cttctcccgt ttccgtcaaa cttattatca
     5401 aacttcttcg atccgtgatc gagagggtcc gctggaccaa cgatcgccct tccaggctgt
     5461 cgatcacgcg agtgaggcgt gaaggcgaaa gatccaattc tttcgccaac gccgaaggct
     5521 cgacgattcc tttctccaca gcacagagaa ggagagcgtc attgaaagaa aggccggttt
     5581 gatctttcaa ctgttcttca aaagagcgca gggcgctttg aaggcggcgt atagcacata
     5641 gattgaacat ctcaacctcc gaatagtttc acttgtcaat aattgcttta gacaactata
     5701 atgataccga cactccttag tcaataagat ataatataac cttataggta aatattattt
     5761 gtataatgtt aaatattctc attaatcctg ctgataatct tatatctact ataaggatag
     5821 acgtttttct cttgactatt atttcgtaat ccttcatgct ggttccaggt tgttctagat
     5881 actgtggcaa agcagtgtcg ggaagaagcc actgagttgc ttccttgttg tacttctaga
     5941 tctacaccgg atggaacaca atatgtagga caccaaggcg tgttgctttt tgcgtttgca
     6001 atcacaccaa atatggtcga tggttattcc caactatcgg ccatttcttt atgtaagcgg
     6061 ttacattatc aaagcagatg tatttgtaga tcaattccca gtatagtaca ggtatgaaaa
     6121 caattctttg ttacggcgat tccaacaccc atggatcgaa ccccggcggg ggacgatggg
     6181 agtatgacaa gcgatggccg ggaatcctgt cttccctgtt gggggatggg tactatgtca
     6241 tcgaagaggg acttggcgga cggacaaccg tcttcgacga tccgttcgag aaaggacgaa
     6301 acggtaaaac ctatcttccc gtcgtgctgg caagccatca gccgattgat cttctcatta
     6361 tttcccttgg tacgaatgat acgaagcgat tttttggcag caatgaacgg atcatcaccc
     6421 gtggaatcga agcgttggcg gatgttgcca aaaccttcga tgtcaaacag atcatggtga
     6481 tctcgccgat ccatgtcgga gcgaggatcg aagagacaat cttcgccggt ttctccaagg
     6541 aatcggtgga aataagctat aagttgagtg aacgggtgaa agcaatggcc gaacgaaaag
     6601 gtctcatctt cttcgatgcc gcgacggcag ccacccccag tgagatcgac cagctccaca
     6661 tggacgcgga aagccaccga agcctggcag aggcgttgaa agaggtgatc attcaacacc
     6721 tgtaaaccgg ctcatcggtt tcggttcact cctgcagcgc ttcgggaaac tcgcccctga
     6781 tccggtcgaa aaaggacagc gtcagaattg tcacccaggc gatgtcgatt tcgtagtggc
     6841 cgttcatcag ctccgggcga ttgaagtact cccagttcgt gtggttgatc tgctccggct
     6901 gccagccgaa gaaggactcg tcccggccga ggtcgttctc tttgccgtga accagctgtt
     6961 tgcacgctgc gatcatcagg gaggcctgct ctgcgtagaa gggaaggttg gcggcggtgg
     7021 caaagcgcag gaactcgtag gcgatcgcca ttccgtaaca atccaggtgg tgatgagcga
     7081 cggaaacgct cgtcatcccc aaggtggaaa atcctttctt tcccaagggg ctctcctggg
     7141 gaaggtagga ggattgctgc cagatccatt ggatgatgaa ctcggctgca aggtgggcgc
     7201 tcttaagata atgccgtttg tttccatcca agtggtataa atccataaag aaggagagga
     7261 gggccacccc cgcctcctta tcgcaggaat cggcatccag cgtatcgccg tagaacgcgc
     7321 cttcatcggc gagccttgca tagaacatat atgctttatg aaccgcatcg atgagaaccg
     7381 ccttgagcgg atcgctttcc tcaagcatcg ggataagggc acaaagaaac gatccgatat
     7441 acgctccgtt ggttccttgg atatctttcg gttttccctc cggggtccac cagcggccga
     7501 aggagccgga agaaagctgg atttcggaat agaagagggc gactcttcgg gcaagatcga
     7561 tatacggtga aacatcatag ccgagctgcc ggagctcctt tgcaagccgg aggtagtgtt
     7621 tcatcgcttc tccgttgcac cggctgttca ccatttcggc gaactcgggg aactccccga
     7681 tcccaaggta tcctccccat tggcccgtcc gaaggtcatg gttgtcctga aacacccctt
     7741 cccggcgttc cgccttgagg aagaaatctc cgatcttctt cgccacggtc accagcaaac
     7801 gggaatcgtc gaccaagctg aaacgcttgg ctaatgctcg tcgtgccgct tgcaggcaat
     7861 cgagggtggg ttcatgttcg gtgaaccggg caagctcgca ggcgccttgg atgcttttca
     7921 ccaaaaagga ggcgccggtg aactcataca catcctgaac ctccccgttg ccctcgccca
     7981 tgatgatata cgccagatta ccttcgcttc ttcttacgag gttgatcagg cgggtgagct
     8041 tcagctccat ccaatggttc caggaaagcg gatggggaag atcggtcggt ttcgcttttg
     8101 gaagggaagc gacgaaagcg acatacacct ccatgccgcg catcccgcgg taggtcgcca
     8161 aatgaaaggt tcgccgatag atcaggccgc cggaaggaac catcaaagcg ggtcggggca
     8221 ttgatgtggt atcgatgaga gctttcttgc cttgatagct gtacggtccc tcccatcccg
     8281 gaatggagat gatcgcccca tgaggatcat tggtcaccga ggcgacgaag cgctcctcgg
     8341 ccgccgcttc ggtggcgcag agaaaatggc gctttccccc ggagagctgg agacaacagg
     8401 gaatcgacat ccttgtttcc agaaatgacc atgttgagga gtgatcgatc gacgggaaca
     8461 gtccgcttcc cttggtgttg tcccggtacc agacggcggg gatgaagagg tcgtttggca
     8521 ctccttcatc ggtggtgaaa aaccccgcct gaagccgata tgatccctct ttcacgagaa
     8581 tccacgtccg gtcgaccgtt acgccgttcc ggccgggagc caagcgatcc tcgatcgtcc
     8641 cgatcccctc gaatgacaca aacaccatgt catcttcaat ctcaagggcg atcgggtgtt
     8701 ggtgtttatc ctcgtaggtg agggtat
//
LOCUS       DAOPUZ010000004         8523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_8303_length_8523_cov_3.423240, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000004 DAOPUZ010000000
VERSION     DAOPUZ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8303_length_8523_cov_3.423240"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKN79_00185"
     CDS             <1..253
                     /locus_tag="PKN79_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNZ94222.1"
                     /translation="VSPQSCLSRNVLTAALSTAVIIGQAPKRSGALHVAGCALDLGKD
                     VFVHQSGIGSIPNQEGSRFLHQSGAPIVGGDDPDQPVVV"
     gene            262..1158
                     /locus_tag="PKN79_00190"
     CDS             262..1158
                     /locus_tag="PKN79_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNZ94223.1"
                     /translation="MIEEQLIEQYLRYLTTVGNLSEHTIVAYRRDLMQLLAFWRQQGV
                     ALEEAALEDGWLYVKHLSKTRRLSEASVNRMISSARTFYDYLLKQERVYSNPFSLIVQ
                     RKARNRLPTILTRDEIQALLALKPSDAESMRDLLLFALLYDTGCRIGEAVSIKEDDLD
                     LKQRRILIVGKGNKQRFVFFGKKVLTRINEYLPVKRNQCASPYLFCSTSGNQLPFSTI
                     GSIFAAYRKKMGWQKEFTPHVLRHSFATHLIDRGADIRLVQELLGHASISTTQIYTHV
                     SQARLSKVVAEIIPTGEGISDE"
     gene            1151..1687
                     /gene="hslV"
                     /locus_tag="PKN79_00195"
     CDS             1151..1687
                     /gene="hslV"
                     /locus_tag="PKN79_00195"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNZ94224.1"
                     /translation="MNEFRGTTIVAIRKDGRVAIAGDGQVTYGDAVLKGNAHKVRRLY
                     DGKVITGFAGTTADAFTLFELFETKLKEYSGDLTRSAVELAKQWRTDRSLRQLQAMMV
                     VSDGKKIYLISGTGDVVDPERDAIGIGSGGNFALAAALAYLDTGADLSAEEIARKSVE
                     VAATLCIYTNDSIITEVL"
     gene            1691..3049
                     /gene="hslU"
                     /locus_tag="PKN79_00200"
     CDS             1691..3049
                     /gene="hslU"
                     /locus_tag="PKN79_00200"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNZ94225.1"
                     /translation="MENSTVRKLDELKPKQIVAELDKYIIGQEAAKRTIAVAIRNRTR
                     RKKLPEEIRDEVSPKNIIMIGPTGVGKTEIARRIAKLSNAPFIKVEATKYTEVGYVGR
                     DVESTIRDLMSVALQQTKAELAEAQAEKVKARVEERLLDLFLPQMKNEPNVDIVAPEK
                     DERLRDRFRTMLREGKFDDRTIELNVQARKHVGIEVMGAPNMEEIQEAMASLGSIFGG
                     KGHVRKVTIKRAREILTEEEMEKAVDNDRAIDEAKERVEQMGIVFIDEIDKVAKSGSS
                     SGIDVSREGVQRDILPIVEGTSVSTKWGVIDTTHILFIASGAFHVSKPSDLIPELQGR
                     FPLRVELNDLTANDFYRILTEPQNALTMQYRELLKTEGVEITFEDAAIRRLSEIAFKV
                     NASHDNIGARRLFTIMERLLEELSFTADELSGQHISITEAYVDERLGEIVEDQDLSRF
                     IL"
     gene            3057..3971
                     /locus_tag="PKN79_00205"
     CDS             3057..3971
                     /locus_tag="PKN79_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94226.1"
                     /translation="MEFLSHTASDYERALREVRSHHGGSVRIHARYDRLEKGKNRCEI
                     HYYLVGTKKQEEEDHASDALATLIAVNDLPYSFIRPVEESLQNHNHSLLEREVEFRLL
                     HHLFDNLRFKEEVREREIYVAGPAGSGKTTMAAKIARFLRLRGQKKIALVEIGSSNDR
                     LLRMLAHDCGVGFERVNDLDEIVRLENYDHLIIDLGNIHDVVPLLSTLQASSQLLVLV
                     FGGSSKVSDMLAVTRLLPQRIQAVLAFAKTDETHTLGNVLALANMANLPLFAFGEGAS
                     VAMGFSLANEQYIFSRLHGFTVELSEFF"
     gene            complement(3955..5601)
                     /locus_tag="PKN79_00210"
     CDS             complement(3955..5601)
                     /locus_tag="PKN79_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNZ94227.1"
                     /translation="MSTSPNHRHINLFIALIGVALLVLAIRFGVLMLKRFQSDKRYED
                     PVVADRVVRGTIYDRNGRILAIQTPYWGVYFHLNKIKDLKLVSEVVAPFVQMSPGEII
                     ERTKAYTTYARISSRIDEDQVEPLLAALAKYNLLDSVNVEKRIGRTYPSLFHAAQILG
                     FTNAEGEGIEGLEYALEKQLNPYPKVGEEQITYGSDVYLTLDMDIQYALDVQLQEIAN
                     LYDPNIIMGIIMDAKTGDILALGSYPWYDPNAFSRSNPRQRTNNTISTLLEPGSVFKL
                     FSLASILKADEAKLDESFECTGSYSFQAGNSTVTINCSTAHGTVDPQMMIAKSCNGAI
                     AHWARQTDEEAFYQTLVDLGFTRSWDIGLPSRPRAQIADPSTWSARSEATIAFGQELL
                     ATSLHLATAATALGIDGDLLQPHLILRIIDASTGTIVKQREKEVVAHLFDRADTALIR
                     KGMHKATSDEGTGYKARVNDFEIGIKTGTAQLFNPEKNSYSDGSTLASSLALAPIEDP
                     AYIIYIGVEGGRGWGADLAAPGIGNLVQALISQGKLRKTR"
     gene            5643..6617
                     /locus_tag="PKN79_00215"
     CDS             5643..6617
                     /locus_tag="PKN79_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ94228.1"
                     /translation="MARDYYDDMHDRNSSRRKRRRGPSKTLLGVIILGIAVCVAVIIL
                     WLQFTTALEVKPAQVIEPQPPAAEEIVPQPVTPPKPSEPVHQAQRLDVRPPSSKSVQF
                     TSHIVQAGEDLHSIALQYGLKVQTLVSINEIRNVQAVTEGAALKIPDRDGRYYTVREG
                     DMLSTIVRTQNLPFGWVTLQELNGLVNEQIKVGDKLFIPDTSSTASSQYTDIAPIQFI
                     RPTPGAIIARFDQESGVRIRTSNDEPIKAAADGEVADTGFEADGQSRYVILSHEGGYR
                     TGYHNLGSVEVIGGSSVVQGESLGLSLEPYLFFSIEQLGIPLDPEQFF"
     gene            complement(6623..>8523)
                     /locus_tag="PKN79_00220"
     CDS             complement(6623..>8523)
                     /locus_tag="PKN79_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNZ94229.1"
                     /translation="DEKQKRVTFTNQGLIHIEELLGKHKVIEGSIYDGQNFEYVHYVT
                     QAVRALRLYHRDVDYVVADGQVQIVDEFTGRVLHGRRYSEGLHQAIEAKERIKVLGQN
                     RTLATITFQNFFRMYDKISGMTGTADTEAPEFLKIYGLDVVVIPTNRPVIREDHPDLV
                     FYNEQFKLQAICQEIARVHKTGQPILVGTISIEKSELLSSLLKRMGIRHEVLNAKNHA
                     REALIIENAGSKGAVTIATNMAGRGTDIKLGGSLEGRIRSQVGSEATREEYQAALEKI
                     APLWKKDYEEVKELGGLYVLGTERHESRRIDNQLRGRSGRQGDPGTSRFYVSLDDPLM
                     RLFASDNVRIILGKIGMQSGEPIEHRMLSNAIEKAQKRVEDRNFEIRKHLLDYDDVLN
                     EQRNFLYRERDAILASDDLVERVRELTRQLIEEEVSRVFAQTKDQKEQAKLLDKVTRT
                     LMLASDPFEGSFTKDAITAHLIESADKEIDEKLKITGNAPLNEFLRFNYLRQIDIRWQ
                     NHLVALEDLRDAVSLRSYAQKNPLVEYKVEGFEIFTQMLDAIGLFVVQTLQRVKITPQ
                     REQYRRTPAQHKVVESHSQQLAFAHEGPEPAKENAVTTIRREGAKVGRNDPCPCGSGK
                     KYKHCHGA"
BASE COUNT         1857 a         2292 c         2442 g         1932 t
ORIGIN      
        1 ggtatcgcct cagagctgtc tgtcccggaa cgttctgacc gccgccttaa gtaccgccgt
       61 catcatcggt caagctccga agagaagcgg agcgctccac gtcgccgggt gcgccctcga
      121 tctgggaaag gatgtctttg tccatcaaag cgggatcggg agcataccca accaggaagg
      181 ctcccgtttc cttcatcaaa gcggagcacc aattgtcggc ggtgatgacc cggatcagcc
      241 ggttgtggta tagtgaacag gatgatagag gaacagctca tcgaacagta tctccggtat
      301 ctgaccaccg ttggcaacct cagtgaacac acgatcgtcg cgtatcggcg tgatctgatg
      361 cagttgctcg ccttttggag gcaacaaggg gtcgcacttg aagaagccgc gttggaagac
      421 ggctggctgt atgtgaaaca cctcagcaaa acccggcgcc tttcggaggc gagcgtgaat
      481 cggatgatca gcagcgcccg aaccttttac gactatctct tgaagcagga acgggtgtac
      541 tccaacccct tttccctcat cgtccagagg aaggcacgga atcgtcttcc gacgatcctt
      601 acccgggatg aaattcaggc cctgctcgcc ctcaagccgt ccgatgccga atcgatgagg
      661 gatcttctgc tctttgcgct gctctacgac accggctgtc ggatcggtga agccgtttcg
      721 atcaaagagg atgatcttga tttgaagcag cgaaggattc tgatcgtcgg aaaaggaaac
      781 aagcagcgat tcgtgttctt cggaaagaag gttctcacgc ggatcaacga atatttgccg
      841 gtgaaacgga atcaatgtgc atccccgtat ttgttctgtt caacatcggg aaatcagttg
      901 ccgttctcga cgatcggcag tatctttgcc gcgtatagga agaagatggg atggcagaag
      961 gagttcactc cccacgttct caggcatagc ttcgccaccc atctcatcga ccgcggtgcg
     1021 gatatcaggc tggtccagga actgcttggt catgcgagca tttccaccac ccagatctac
     1081 acccacgttt cccaagcgcg cctgagcaag gtcgtggccg aaatcatccc cacgggagaa
     1141 ggaataagcg atgaatgaat tcagaggaac aacaatcgtg gcgatccgaa aagacggccg
     1201 cgtggcgata gccggtgacg gacaggtcac ctatggcgat gcggtgctga aaggcaatgc
     1261 ccacaaggtc cgccgcctct atgacggcaa ggttataaca gggttcgccg gaaccaccgc
     1321 cgatgcgttc accctctttg agctctttga aacgaagctg aaggagtaca gcggcgatct
     1381 tacccgaagc gccgtcgaac tggcgaagca gtggcggacc gaccgctccc tgaggcagct
     1441 gcaggcgatg atggtcgtca gtgacggcaa gaagatctat ctgatcagcg gtaccggcga
     1501 tgtggtcgac cctgagcggg atgcaatcgg catcggcagc ggcggaaact tcgcccttgc
     1561 cgccgcattg gcttacttgg acaccggtgc ggatctcagc gccgaggaga tcgcccgcaa
     1621 gagtgtcgaa gtcgccgcca cgttgtgtat ttataccaac gattcaatca tcaccgaggt
     1681 attgtaaggt atggaaaatt ccaccgttcg aaagctggac gagctcaagc cgaagcagat
     1741 cgtcgccgag cttgataagt atatcatagg ccaggaggcc gccaaacgga cgatcgcagt
     1801 ggcgattcgc aatcgtaccc gccgaaagaa gcttcccgaa gagattcgcg atgaagtttc
     1861 accgaagaat atcatcatga tcggccccac gggggtcggc aagacggaga tcgcccgccg
     1921 aatcgccaag ctgtccaacg ctccgttcat caaggtggaa gcgacaaaat acaccgaagt
     1981 agggtatgtc ggacgggacg tggagtcgac catccgcgat ctgatgagcg tagctctcca
     2041 acagacgaag gcggagcttg ccgaggctca ggcggaaaaa gtgaaagccc gggtggaaga
     2101 acggctgttg gatttgtttc ttcctcaaat gaaaaatgaa cccaatgtgg atattgtcgc
     2161 tcctgaaaag gatgagcgct tgcgcgatcg gtttcggacg atgctccgcg agggcaagtt
     2221 cgacgaccgg acgatcgaat tgaacgtgca ggccagaaag catgtgggga tcgaggtgat
     2281 gggcgccccc aacatggagg agattcagga agcgatggcg agcctcggtt cgatcttcgg
     2341 cggcaaaggg cacgtgagaa aagttacgat caagcgcgcc cgggaaatcc tcactgaaga
     2401 ggagatggag aaagcggtcg acaacgaccg ggcgatcgat gaagcgaagg agcgggtcga
     2461 acagatgggg atcgttttca tcgatgagat cgacaaggtc gccaaaagcg gatcttccag
     2521 cggtatcgac gtatcccggg agggggttca gcgggacatc cttccgattg ttgaaggaac
     2581 gagcgtatca acaaagtggg gagtcatcga caccacccac atccttttca tcgccagcgg
     2641 agcgttccac gtctccaagc ccagtgatct gattcccgaa cttcaggggc gatttcccct
     2701 tcgggttgag ctgaatgatc tgacggcgaa tgatttctat cgaatcctca ccgaaccaca
     2761 gaacgccttg acgatgcagt atcgagagct tttgaagacg gagggcgttg aaatcacctt
     2821 tgaagatgcg gcaatccgcc ggctcagtga aattgctttt aaagtgaacg cttcacatga
     2881 taacattgga gctcgacggt tgttcacgat catggagcgg cttttggagg agctttcctt
     2941 cactgccgat gagctttccg gacagcatat ttcgataacc gaagcgtatg tcgacgagcg
     3001 ccttggcgag atcgtcgagg atcaggacct tagccggttc attctgtagg agtgatatgg
     3061 aatttctgag ccacaccgcc agcgactatg aacgagccct tcgggaggtt cgttcacatc
     3121 atggtggcag cgttcggatt catgcccgat atgatcgtct cgaaaaggga aagaaccggt
     3181 gtgaaatcca ctattacctc gtaggaacga agaagcagga agaggaagac catgcctccg
     3241 atgccttggc gaccttgatc gccgtgaacg atcttccata ctcgttcatc cgaccggtgg
     3301 aggagagcct tcagaaccat aatcattcgt tgctcgaacg cgaggtcgag tttcggctgc
     3361 ttcaccacct cttcgacaac ctccgcttca aagaggaggt tcgagagcgg gaaatctatg
     3421 tcgccggacc tgcaggaagc ggaaagacca cgatggcggc gaagatcgcc cgtttccttc
     3481 gtcttcgcgg tcagaagaag atcgccctcg tcgagatcgg atcgtcaaac gatcgcctgc
     3541 tgaggatgct tgcccatgat tgcggggtcg ggtttgaacg ggtcaacgat cttgatgaaa
     3601 tcgtccgctt ggaaaactac gatcatctga tcatcgatct ggggaacatc catgacgtcg
     3661 tgccgcttct ttcaacgctc caagcctcct cacagctgct ggtcttggtg tttggcgggt
     3721 cctccaaggt cagcgacatg cttgcagtta ccagactcct tccgcagcgg attcaggccg
     3781 ttctcgcctt cgccaaaacc gatgagaccc ataccctggg caacgtgctt gccctcgcca
     3841 acatggcgaa tcttcccctc tttgccttcg gcgaaggggc gagcgtggcg atgggcttca
     3901 gcttggcgaa tgagcagtat atcttcagtc ggcttcacgg attcaccgtc gagctcagcg
     3961 agtttttctg agcttgcctt ggctgatgag cgcttgaacg aggttgccga tcccgggtgc
     4021 cgctagatct gcgccccacc cgcggccccc ttcgacaccg atgtagatga tgtaggcggg
     4081 atcttcgatc ggggcgagtg ccaagctgga agcaagggtc gatccgtcac tgtaggagtt
     4141 cttctcggga ttgaagagct gagcggtgcc ggttttgatc ccgatttcga aatcgttcac
     4201 ccgcgctttg tatccggttc cctcatcgct cgtcgctttg tgcatccctt ttcggatgag
     4261 cgcagtgtcc gcccggtcaa aaaggtgggc gacgacctcc ttttcccgtt gtttcacgat
     4321 ggttccggtg cttgcatcga ttatacggag aatcaggtga ggctgaagaa gatctccgtc
     4381 aatcccaaga gccgtggcgg cggtggcgag gtggagggag gttgctaaaa gctcctggcc
     4441 gaaggcgatc gtcgcttcac tgcgggccga ccacgtcgaa ggatcggcga tctgagcgcg
     4501 cggtcgggag gggaggccga tatcccagct tcgggtgaac cccagatcga caagcgtttg
     4561 ataaaaagcc tcctcatcgg tctggcgggc ccagtgggcg atcgctccgt tgcaggattt
     4621 ggcgatcatc atctgcggat caacggttcc gtgggcggtg gagcagttga ttgtgacggt
     4681 cgagttgccg gcttggaagg agtagctgcc ggtgcactcg aagctctcgt caagcttggc
     4741 ttcgtcggct ttgagaatcg aagcaaggct gaagagcttg aagaccgacc ccggttcaag
     4801 aagggtgctg atcgtgttgt tcgtccgttg gcggggattt gagcgactga atgcattggg
     4861 atcataccac gggtagcttc caagggcgag gatgtcgccg gtttttgcat ccatgatgat
     4921 ccccatgatg atattgggat catagagatt cgcgatctcc tgaagctgga cgtccaaggc
     4981 atattggata tccatatcca aggtgagata cacatcgctt ccataggtga tctgttcctc
     5041 tccgactttt gggtagggat tgagctgttt ttccagagcg tactcgagtc cttcaatccc
     5101 ttcaccttcg gcattggtga agccgagaat ctgagcggcg tgaaagagag aagggtaggt
     5161 tcgaccaatc cgtttctcga cgttgacgga atcgaggaga ttgtacttag cgagcgccgc
     5221 caacagaggc tccacctgat cttcatcaat tcgactgctg atccgggcat acgtggtata
     5281 ggcttttgtc cgctcgatga tttcaccggg gctcatttgg acgaagggag cgacgacttc
     5341 gctgaccagc ttcaaatcct tgatcttgtt gagatgaaag tagactcccc aatagggagt
     5401 ctggatcgcc aggatacgcc cgtttcgatc gtagatcgtc cctcgaacga cccggtcggc
     5461 gacgacggga tcctcgtatc gtttatcgct ttggaagcgt ttgagcatca aaaccccgaa
     5521 tctgatcgcc aaaacaagca acgccacccc tatgagggcg atgaagaggt tgatatggcg
     5581 atgatttgga ctggtagaca tggtcgattt atactactat gggggagagg cattgtcgag
     5641 atatggcgcg agactactat gatgatatgc acgaccggaa ttcctcaagg agaaaacgga
     5701 ggcgtggacc gagcaaaacc ctcctgggcg tgatcattct cggtatcgcc gtctgtgtcg
     5761 cagtgatcat cctgtggctt caattcacta ctgccttgga ggtaaaaccc gctcaagtca
     5821 tcgagccgca gccccctgcc gccgaagaga tcgtcccgca gcccgttact cctcctaaac
     5881 cgagcgaacc ggtacatcaa gcgcaacgac tcgatgttcg gcctccttca agcaagagtg
     5941 ttcaattcac ctcccacatc gtacaagcgg gcgaagactt gcattcaatc gctctccagt
     6001 atggtttgaa agtgcagacg ttggtatcga tcaacgagat caggaacgtc caagcggtta
     6061 ccgaaggagc tgcgctgaag attcccgatc gggatggccg ttattatacg gttcgagaag
     6121 gggatatgct ttccacgatc gttcggacgc agaatcttcc attcggctgg gtgacgctcc
     6181 aagagctgaa cggtctggta aatgaacaga tcaaggtcgg cgacaagctt tttattcccg
     6241 acacctcttc caccgcttct tcgcaataca ccgacatcgc tccgattcaa ttcatccgac
     6301 cgacaccggg ggcgatcatc gcacggtttg atcaggaaag cggagttcga atccgaacct
     6361 ccaatgacga gccgataaaa gccgccgccg acggcgaagt cgccgatacg gggtttgaag
     6421 ccgacgggca aagccggtac gtaattctca gtcatgaggg cggctatcgc accggctacc
     6481 acaacctcgg cagtgttgaa gtgatcggtg gcagctcggt cgttcaggga gaatccctcg
     6541 gtctgagcct cgaaccctac ctcttcttct cgatcgaaca gctcggcatt cccttggatc
     6601 ccgaacagtt tttctagcgg tctcacgctc cgtgacagtg tttgtacttc tttccgcttc
     6661 cgcaggggca ggggtcgttt ctccctacct tcgctccctc gcgccggatc gtggttaccg
     6721 cattctcctt tgccggctcc ggtccttcat gggcgaaggc gagctgctgg gaatgggatt
     6781 ccaccacctt gtgttgagcg ggagtgcgcc ggtattgttc ccgctgggga gtaatcttca
     6841 cccgctggag ggtctggacg acgaacaacc cgatcgcatc gagcatttgg gtgaaaatct
     6901 cgaacccctc caccttgtat tcaaccaagg ggtttttctg ggcatatgac ctgagactca
     6961 ccgcatcccg cagatcctca agcgccacta aatggttttg ccatcggata tcgatttgac
     7021 gcaggtagtt gaagcggagg aactcgttca agggggcgtt gcccgtgatc ttgagcttct
     7081 catcgatctc tttatcggcg gattcgatca gatgggccgt gatggcgtcc tttgtgaaag
     7141 agccttcgaa ggggtcggag gcgagcatca gggttcgggt gaccttgtcc aggagcttcg
     7201 cctgttcctt ctgatccttc gtctgagcga agacccggga aacctcctcc tcgatcaatt
     7261 ggcgggtcag ttccctcacc cgttcgacaa ggtcatcgga tgcaaggatt gcatcccgtt
     7321 cgcggtagag gaagttccgc tgctcgttga ggacgtcgtc ataatcgaga aggtgctttc
     7381 gaatctcgaa gtttcgatcc tcgacccgtt tttgagcttt ctcgatcgca ttcgagagca
     7441 ttcggtgctc gatcggctca ccgctttgca tcccgatctt ccccaggatg attcggacat
     7501 tgtccgaagc gaacaggcgc atcaaaggat cgtcgaggct gacatagaat cggctggttc
     7561 ccggatctcc ctgccggccg cttcgaccgc gcagctggtt gtcgattcgc cttgattcat
     7621 gtcgttccgt tccgagaacg tacaaacctc cgagctcttt gacctcttca tagtcttttt
     7681 tccagagcgg agcgatcttc tccaacgcag cttggtactc ttctcttgtg gcctcggagc
     7741 ccacctggga acggattcga ccttccaggc ttccgccgag tttgatgtcg gtgcctcgac
     7801 ccgccatatt ggtagcgatc gtgacggcgc ctttggagcc ggcattttca atgatgagcg
     7861 cctcccgggc gtggttcttg gcgttgagca cttcatgcct gatccccatc cgcttcagga
     7921 gcgaggagag caattcgctc ttttcgatcg agatcgttcc cacgagaatc ggctggccgg
     7981 ttttatggac gcgggcgatc tcctggcaga tcgcttgaag cttgaactgc tcgttgtaga
     8041 agacgaggtc cggatgatct tcccggatca ccggtcggtt ggttggaatg accacgacat
     8101 caaggccgta gattttgagg aactcgggag cctccgtatc ggcggttccc gtcatcccgc
     8161 tgatcttgtc gtacattctg aagaagtttt ggaaggtgat cgtcgccagc gtcctgttct
     8221 gaccgaggac tttgatccgt tccttcgctt cgatcgcctg gtgaagaccc tcgctgtagc
     8281 gccggccgtg cagcacccgg ccggtgaact cgtcgacgat ttgaacctga ccgtcggcga
     8341 cgacgtagtc gacgtccctg tgatagagcc gaagcgcacg gaccgcctgg gttacatagt
     8401 ggacatattc gaagttctgc ccatcgtaga tcgagccttc gatcaccttg tgttttccaa
     8461 gaagctcttc gatgtggatg agcccctgat tggtgaacgt gacccgcttc tgtttttcat
     8521 cga
//
LOCUS       DAOPUZ010000005         7492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_9850_length_7492_cov_3.176281, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000005 DAOPUZ010000000
VERSION     DAOPUZ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9850_length_7492_cov_3.176281"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1340
                     /locus_tag="PKN79_00225"
     CDS             198..1340
                     /locus_tag="PKN79_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNZ94230.1"
                     /translation="MNTIYLDNGATSWPKAPTVASTMASFITEEAVNIGRGGYDRAYE
                     TAGRIDECREKLGAFFGAPEDRNVTFSLNVTHALNTLIAGLFTKDDHVMVSGMEHNAV
                     MRPLSHLDIPYSIIPCDREGRIHLEQIPHLINGKTKAIIMTQASNVTGTIQPIKELSS
                     IAGKYGLLLLIDAAQLPLSNPLSIIKDNLDAIAFTGHKSLLGPQGVGGLVLSDRLAKE
                     VRPWACGGTGSKSDLLEMPDFLPDKFEAGTQNLPGIIGLSAALDFIKDNSESIIKNEY
                     ETTELLLSYFLSDKRIQVIGPKDMKDRTSVISVDFPTLDNARIAYELFLDAGIETRVG
                     LHCAPVAHKTIGTFPRGTLRFSPGYATTKQEILRTIESCKRVLDRL"
     gene            complement(1498..1758)
                     /locus_tag="PKN79_00230"
     CDS             complement(1498..1758)
                     /locus_tag="PKN79_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94231.1"
                     /translation="MVIKHKDEIMWFIIAFVVVLILVLAFKSNKKEETITTTDQETGK
                     TTVETRVTNTPSAGQTAARIVLLIVAIIVAVLFLLVVCASMM"
     gene            2033..2800
                     /locus_tag="PKN79_00235"
     CDS             2033..2800
                     /locus_tag="PKN79_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ94232.1"
                     /translation="MHDVDFSAESSQIIALIGPNGCGKSTMLRTLSGQIKPQKGSVSI
                     CGQELSSLRQKELSKCVAFLPQRHEAVHGVSVHELVAMGRAPYHRSGWILSMKDKEKI
                     KWALSYMNLEDYVSRPVDQLSGGEQQRVWIAMALAQDTPIILLDEPVTYMDLRFQWEL
                     LSTIGDLRDTHGKTIVSVFHDINHAIEVADHIYLFKDGRVYAQGTSEEVVTEDNIGDV
                     YGLHAHVCRFNRCRRNVVVPTSLNPRFKRRHGNRVVM"
     gene            2876..3787
                     /locus_tag="PKN79_00240"
     CDS             2876..3787
                     /locus_tag="PKN79_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ94233.1"
                     /translation="MMISLLVSPLFAQGAKEQTVRTEIETKVVVDELSRSVTIPQNPQ
                     RVVALTSAVMQSLYNIGISPVGKVEEYRVSKEGMALPSVGKTSAINIEAVYALNPDLI
                     IASSRFHSALKESLEQSGAAVYFFDPDKVGEISVVDLNVYLGKLLDREEEGRKYVESI
                     YSLARDLRTEIVEPLGIGTAVMIQDGDAITAAQTASSYGAMLKLLGLQNIVPGNLPGA
                     GRGSFVKYSIETILQQHPDVIFLLASSNDEASNRAMLEKFKHDEQWKHYTMQGGRFVL
                     LPFLANPNRTSVENMLRIAADALKKND"
     gene            3796..4806
                     /locus_tag="PKN79_00245"
     CDS             3796..4806
                     /locus_tag="PKN79_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNZ94234.1"
                     /translation="MKRFHSHRLGVLGAAAILLVFIIALSLSVGSVQVSLGNIVGILF
                     GKKGERLPVQIVRNIRLPRTLVGLLVGMNLAVSGVLLQGIIRNPMASPNIIGVNAGAG
                     LAAVLVMALFPSRIGFIPPSSFIGALGAALTINALANSKGGGRKSTVYIVLAGVAVSN
                     LLNAGTSALMIIYSDILEVSYSWLLGSLSGRSWSSFALIWPYSLAGLAFALFLSPKIN
                     LFSLGDDVASSVGLSVRLYRFLALLLAAVLAGSAVSVTGTIGFVGLIAPHAARLLVGN
                     DHRYALPLSALLGAILLILSDTFARTLFSPVELSVGIITSLLRAPFFLFLLFRKRKAS
                     VL"
     gene            complement(4808..5605)
                     /gene="zupT"
                     /locus_tag="PKN79_00250"
     CDS             complement(4808..5605)
                     /gene="zupT"
                     /locus_tag="PKN79_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNZ94235.1"
                     /translation="MDNVLFAFSLTLFAGLATGVGSLLAFLSKKFNPKFLAGALGFSA
                     GVMIYVSFVEIFVKARDSLSIPFGETKGYWVTTIAFFGGIALIAIIDRLIPSYENPHE
                     IGNINEPVDKASEQRLLRLGVFSALAIAIHNFPEGLATFMGALSDPTLGISIAVAIAI
                     HNIPEGMAVSVPIYYATKSRKKAFWLSFASGIAEPLGALVGYFILKNFISDSTFGIVF
                     AGVAGIMVYISLDELLPTAEEFGEHHIAIGGLIAGMLVMAISLLLFA"
     gene            5826..6842
                     /locus_tag="PKN79_00255"
     CDS             5826..6842
                     /locus_tag="PKN79_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ94236.1"
                     /translation="MTHNKTQTGNLFLVIFIIMAASCLVGCGAQKKPAQFEEHDGIIV
                     GFSQIGAESAWRTCNTRSIQEAAAERGVQLVYDNAEQKQENQIKALRSFIAYQVDVIV
                     FVPIVTDGWDNVLQEAKDAGIPVLVTDRKIRVEDPSLYSGFIGTDSITEGRNGGLFLL
                     NKFKAERDRFEETGEYINIVEMFGTEGSSVAIGRAEGFRQILDDHPEFRIIYSKSGDF
                     LRSRGYELAVEFLEQYEDIDVIFSHNDGMTLGAIEAMEERGIRPGIDIVIVTIDAQQA
                     AIDALREGKVNCVIECNPKTGPDIINLAEKLAAGESIPKLQYVHEEVFYETDDLSLVA
                     PRGY"
     gene            6856..>7492
                     /locus_tag="PKN79_00260"
     CDS             6856..>7492
                     /locus_tag="PKN79_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HNZ94237.1"
                     /translation="MTLIDKIFSAFKIRSIKESIKISYVIIILLMITPPVITIGSFVI
                     QMVRYDLIITNVSKANRLNQTVKMDVSNEIWDIVAGNKKFNEGDQYKIIDGINESLED
                     IMSTTEERENRQMLEVAGRAVDTLKRNVDRLGSQMASSSPVSENEEILEEIRGVSALI
                     SDILQDFIVRVIESATITNEHHKRITLVLTVIQVFTVFLVAIFAVFTQRSVT"
BASE COUNT         1827 a         2018 c         1959 g         1688 t
ORIGIN      
        1 cgatgcagaa ccccatgttc gtgggattcc cgagcaggac cagaatcacg gatatgattc
       61 ccgtgatcgc tccggcgatg atgagcgatt gcttctcttt tgaaatcttc atatggcctc
      121 caaggtttcc acattataac cattatttat ccaaatatac aatgggaatt ttcattccgt
      181 tatgctagca ttgagaaatg aacacgatct atctggataa cggggccacc tcttggccca
      241 aggctccgac cgtagcgagc acaatggcat cattcatcac tgaagaggcg gtcaacatcg
      301 gcaggggcgg ctatgaccgg gcctacgaga cggccggtcg gatcgatgag tgcagagaaa
      361 agctgggggc cttcttcgga gctccggagg accgcaacgt caccttcagc ctcaatgtca
      421 cccatgcgct caataccctg attgcaggtc tgttcacgaa agatgaccat gtcatggtga
      481 gcgggatgga acacaatgcg gtgatgaggc cgctctctca tctcgatatc ccctattcga
      541 tcatcccgtg cgaccgggaa ggcagaatcc accttgagca aatcccccat cttatcaacg
      601 gaaaaacgaa agcgatcatc atgacgcagg cgtcgaatgt aaccggcacg attcaaccaa
      661 taaaagaact ctcttcgatt gccgggaaat acggactgtt gctcctcatc gatgccgccc
      721 agctgccgtt atccaatccc ctttcgatca tcaaagacaa tcttgatgcg atcgcgttca
      781 ccgggcacaa gagcctcctc ggtccacagg gcgtcggcgg attggtcctg tccgaccgat
      841 tggcgaaaga ggtcagacct tgggcgtgcg gcggcaccgg cagcaaaagc gacctgcttg
      901 agatgcctga tttccttccc gacaagttcg aggcgggaac ccagaacctg ccgggaatca
      961 tcggcctgtc cgcagcgctg gactttatca aggacaacag cgagtcgatc atcaaaaacg
     1021 aatacgaaac caccgagctt ctgctttcct atttcctctc agacaaacgc atacaagtga
     1081 taggtccgaa ggatatgaag gatcgcacga gcgtgatcag cgtcgacttt ccaaccttgg
     1141 acaatgcccg gatcgcctat gagctgttcc ttgatgccgg catcgagacg cgagtgggtc
     1201 tccactgcgc ccccgtcgct cataaaacaa taggcacctt cccccgaggt accctccgct
     1261 tcagtcccgg atatgcgacc acgaaacagg agatcctcag gacgatcgaa tcctgcaaaa
     1321 gagtgttgga caggttataa acggaagaaa ctgctctcga atagtataag attggaaagc
     1381 aattaaagag aaatcatagc agccgttctt catgttaata aaacgggatg tatgacgaat
     1441 ctaacaaaag tggtattcac ttctttctta tataaagctc ccttcagggc acagcgatta
     1501 catcatactc gcacaaacta caaggagaaa caaaaccgca actataatag cgacaatcaa
     1561 gagaacgatt cttgctgccg tctgcccggc actaggagta tttgttaccc gagtctcaac
     1621 ggttgtcttt ccggtttcct gatcagttgt agtaattgtc tcttccttct tattggattt
     1681 gaacgctaat accaatataa gaacgacaac aaaagctatg ataaaccaca taatctcatc
     1741 cttgtgtttg ataaccatga agagtacatc aagttgcttg atacaatcat atcacatctg
     1801 cctaataatt taagtttttt gtttctaatt taaaatctat gaactcttta tccatggagc
     1861 cagttcatcc atgaacagtt ccggcagtcc actctgaaca cttttacctg tattgacatt
     1921 tgtctggtgg aatgggtatc atggagtaaa atgttagcca aggctaataa ataggggtat
     1981 tgtcttgatt tcggtacatg gtctgtctgt cggatatgga aagcgcacca tcttgcacga
     2041 tgtcgatttc agcgctgaat catcccaaat catcgctctg atcggaccca acggatgtgg
     2101 aaaatcaacg atgttgcgga ccctgagcgg acaaatcaaa ccccagaaag gcagcgtgag
     2161 catctgcgga caggaactat cttctctgag gcaaaaggag ctgtcgaagt gcgtagcctt
     2221 cctgcctcag aggcatgaag ccgtgcacgg tgtttccgta catgagctgg tagctatggg
     2281 aagagccccc tatcacagaa gtggctggat tctctcgatg aaggacaagg agaagatcaa
     2341 atgggccctc tcctacatga atttggaaga ttatgtgtcg cgacctgtgg accagctttc
     2401 gggtggtgag caacaacggg tttggatcgc catggcgctt gcccaggaca ccccgatcat
     2461 ccttctggat gaaccagtca cgtacatgga tctgagattc cagtgggagc tgctgagcac
     2521 cattggcgac ctgagggaca cccatggaaa aacgatcgtc agcgtctttc atgacattaa
     2581 ccatgcgatt gaggtcgccg atcatatcta cctcttcaaa gacggcaggg tttatgctca
     2641 agggacgagc gaagaggtgg tcacggaaga caacatcggc gatgtctacg gccttcacgc
     2701 ccacgtctgc agattcaacc ggtgccggcg caacgtcgtg gtgcctactt ccttaaaccc
     2761 gagattcaaa cggagacatg ggaatcgggt tgtgatgtag attgaaacac atgcacaaaa
     2821 cacacaagga gattgtgtca tgaaaaatat attcgtacat acatccgttg tattcatgat
     2881 gataagcttg cttgtctcgc ctctcttcgc ccaaggtgca aaggagcaaa ctgtccggac
     2941 cgagatcgag acgaaggttg tcgtcgatga gctttcccgt tccgtgacga ttccccaaaa
     3001 cccccagcgg gtcgtggccc tcacctcagc ggtgatgcag tcgctgtaca acatcggtat
     3061 ttccccagtc ggcaaagtgg aggagtaccg ggtgtccaag gaagggatgg ccctgccttc
     3121 ggtcggcaag acctctgcga tcaacatcga agcggtgtat gcattgaatc ccgatctcat
     3181 catcgccagc agcagattcc attcggcgtt gaaggaatcc cttgagcaaa gcggagccgc
     3241 tgtgtatttc ttcgatccgg acaaggtggg agaaatctcc gtcgtggatc tgaacgttta
     3301 cctcgggaag ctgctggaca gggaggagga aggcaggaaa tacgttgagt cgatctactc
     3361 cctcgcccgg gatctgagaa ccgagatcgt cgagccttta gggatcggaa ctgcggtgat
     3421 gattcaggat ggggatgcca taacggcggc ccagaccgcc tcttcctatg gcgccatgct
     3481 caagctgctt ggtcttcaga acatcgttcc cggaaatctt cccggagccg gcagaggctc
     3541 cttcgtgaag tacagcatcg aaacgatcct gcagcaacat cccgatgtga tcttcctcct
     3601 cgcatcgagc aacgatgagg caagcaatcg ggcgatgctt gaaaagttca agcatgatga
     3661 acaatggaaa cactatacga tgcaaggtgg acggttcgtg ctccttccct ttttggcgaa
     3721 tcccaaccga acctccgtcg agaacatgct cagaatcgcc gcggatgcgt tgaagaagaa
     3781 cgactgaatt cccctatgaa gcgttttcac tcccatcggc tcggagtgct tggtgcggcg
     3841 gcgattctcc tggtgttcat catcgccttg agtctttccg tcggctccgt gcaggtgtct
     3901 cttggaaata tcgtcggcat cctcttcgga aagaagggcg agagactgcc tgtccagatc
     3961 gtgaggaaca tccggcttcc gagaaccctt gtcggtctgc tggtcggcat gaacctcgcc
     4021 gtttcgggcg tcctgctgca aggcatcatc cgcaatccca tggcctcgcc gaacatcatc
     4081 ggcgtcaatg ccggagccgg tctggcggcg gttctggtga tggcgctgtt tccaagtcgc
     4141 atcggcttca tccctccgtc ctcgttcatc ggtgcgttag gcgcagccct gacgatcaac
     4201 gcccttgcaa actcgaaggg aggaggaaga aaaagcaccg tctacatcgt ccttgccggc
     4261 gtcgcggtct ccaacctcct gaacgcgggg acctccgcgt tgatgatcat atacagcgat
     4321 attcttgagg tatcctactc ctggcttctc gggagcctct cgggaagaag ctggtcgtcg
     4381 ttcgccctga tctggccgta cagtctggcc gggctcgcgt tcgcgttgtt cctgagtccg
     4441 aagatcaatc tcttcagtct gggtgatgac gtcgcttcca gtgtgggact atccgttcgg
     4501 ttgtatcggt ttctcgccct gctgctcgcc gccgtgctgg cggggagcgc cgtcagcgtg
     4561 acgggtacga tcgggttcgt cggtctgatc gccccgcatg cggccagact gctggttgga
     4621 aacgaccacc gttacgccct gccgctctct gcgctgctgg gcgccattct gctgattctt
     4681 tccgacacgt ttgcccggac gctgttctcc ccggtggagc tttcagtggg gatcatcacc
     4741 tccctcttgc gagccccgtt cttcttgttc ctgctcttca ggaaaagaaa agcctcggtt
     4801 ctctagatca ggcgaacaga aggaggctga tcgccatcac gagcattcct gcgatgagtc
     4861 ctccgatcgc gatgtggtgt tctccgaact cctctgccgt cggcagcagc tcgtccagtg
     4921 aaatatacac catgatgccc gcgactccgg caaagacgat cccgaaggtc gaatcgctga
     4981 tgaaattctt caggatgaag tagccgacca gcgctcccag cggttcggcg atcccggagg
     5041 cgaaggagag ccagaacgcc ttcttcctgc tcttcgtcgc ataatagatc ggaacggaca
     5101 ccgccatgcc ttcaggaatg ttgtggatcg ctatggcgac tgcgatgctg atgcccagcg
     5161 tgggatcgga aagcgctccc atgaaagtgg caagtccttc cggaaagttg tggatggcga
     5221 tcgccaatgc cgagaacacc cccagcctga ggagccgctg ctcggatgcc ttgtcgaccg
     5281 gttcgttgat gtttccgatc tcatgagggt tctcatagga aggaatcagc cggtcaatga
     5341 tcgcaatcag agcgattccg ccaaagaagg cgatcgttgt gacccagtag cctttcgtct
     5401 ctccgaacgg gatgctcagc gaatcccggg ccttcacgaa tatctcgacg aaggagacgt
     5461 aaatcatcac ccctgcggaa aatcccaacg ccccggcaag aaacttggga ttgaatttct
     5521 tggagaggaa agccaacagg ctcccgactc cggtagcaag accggcaaat aacgtcagac
     5581 tgaacgcgaa caatacatta tccataccat ctccaacact tcggcaaaca tatatactac
     5641 cctatcatgt tcaaacgaat cttcctactc gatcccaatg agttagctta tgctaactta
     5701 ttgatcttgt aaagcccccc tcgtggtatc acgtgcgtgt tcgatccacc catatcattt
     5761 tcttgtggat tcccgtgttg gtgatatata atgagttaac gtgcacgcaa aaaaggggca
     5821 tgtcgatgac tcataacaaa acgcaaaccg gaaacctgtt ccttgtcatc tttatcatca
     5881 tggccgcatc ctgccttgtc ggatgcggcg ctcagaagaa gcctgctcaa tttgaagaac
     5941 atgacgggat catcgtaggg ttctcccaga tcggtgctga aagcgcctgg agaacatgca
     6001 acacccgctc cattcaggag gctgcggccg aacggggggt gcagctggtg tacgacaacg
     6061 ctgaacagaa acaggaaaac cagatcaaag ccctgcgttc gttcatcgcc tatcaggtcg
     6121 atgtgatcgt cttcgtgccg atcgtcacgg atgggtggga caacgtgctt caggaagcca
     6181 aggatgccgg aatacccgtg ttggtcaccg accggaagat ccgtgtcgag gatccctcgc
     6241 tctattcggg attcatcggc accgacagca tcacggaagg ccggaacggt ggactgttcc
     6301 tattgaacaa attcaaagcc gaacgcgacc ggttcgagga aaccggagaa tatatcaaca
     6361 tcgtggagat gttcgggacg gaaggctcct ccgtcgctat cggacgagct gaagggttcc
     6421 ggcagattct ggatgaccat ccggagttcc ggatcatcta cagcaagtcg ggtgatttcc
     6481 tccgttccag ggggtatgag ctggccgtcg agtttctcga acaatatgaa gatatcgacg
     6541 tcatcttttc ccataacgac ggaatgactc tgggagccat cgaggcgatg gaagaaagag
     6601 gaatccgacc cggaatcgac atcgtcatcg tcacgatcga tgcccagcag gcggcgatcg
     6661 atgcgctccg tgaaggcaag gtgaactgcg tcatcgagtg caacccaaag accggtcccg
     6721 acatcatcaa tctggctgaa aaactggcag cgggtgaaag cattccgaaa ctgcaatacg
     6781 tgcatgaaga agtgttctat gaaaccgacg acctctccct cgtcgcgccc cgcgggtatt
     6841 agcatgaaga atcggatgac ccttattgac aagatcttct cggcgttcaa gatccgaagc
     6901 atcaaggaga gcataaagat ctcctacgtg atcatcatcc tcctgatgat cactcccccg
     6961 gtcatcacga tcggctcctt cgtcatccag atggtcagat acgacctcat catcaccaac
     7021 gtcagcaagg cgaaccggct caaccagacg gtgaagatgg atgtctccaa cgaaatatgg
     7081 gacatcgtgg cgggaaacaa gaagttcaat gaaggcgatc aatacaagat catcgacgga
     7141 atcaacgaga gtctggaaga catcatgagc accaccgaag agcgggaaaa ccgacagatg
     7201 ctcgaggtgg ccggccgggc ggtcgatacg ctgaagcgga acgtggatcg cttgggaagc
     7261 cagatggcga gcagttctcc ggtgagcgag aatgaggaga tcctcgagga gatccgggga
     7321 gtgtccgccc tgatctcgga catcctccag gatttcatcg tgcgggtgat cgagtctgcg
     7381 acgattacga atgagcatca caagcggatc accctcgttc ttacggtcat ccaggttttt
     7441 accgtctttc tcgttgcgat cttcgccgtc ttcacccagc gctccgtaac cg
//
LOCUS       DAOPUZ010000006         7390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_10024_length_7390_cov_3.500477, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000006 DAOPUZ010000000
VERSION     DAOPUZ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10024_length_7390_cov_3.500477"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKN79_00265"
     CDS             <1..314
                     /locus_tag="PKN79_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ94238.1"
                     /translation="FKTHQGVAGVGTFLFNTYINDDWTTIHCEKGDLAYSVLDLNQPI
                     VIRTKEGTETIAGDPPPTHVAQPLIRTVVDELLGIGTCPSTGESALRTDWVLARLAGT
                     I"
     gene            complement(311..1393)
                     /locus_tag="PKN79_00270"
     CDS             complement(311..1393)
                     /locus_tag="PKN79_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94239.1"
                     /translation="MDKDLLTELYSLAKATFTANLDGLFFEEDLFAVSLANRPTYYVT
                     LGNGVLVANRGKKGLTNFLEYLEHEETEDELEFFEYQQAMDSLYSVVPFDEDDDMLET
                     DETWRKESGVLFEDMAAPVFRSKVEYCLPGIIDEEEAKEFIRIFKATLHLIDRLTEIK
                     KEHPKHRAIAMWHDQVSDGSKKNEYIPRLTVGENEIVLDKLLFEPWNYENTFPVPEFA
                     DAEQRRYYKSMKAKSGKVFYMATGIFPAPVSSEKFDEPTFPVFYLLYDPQAHQILDVY
                     MLDDYEKDHNIFIDRFLAVVDRGVKPQAIHCYGRRTLPLLTKIGPNMGVMVFEGKRDA
                     ELDEVIDDMLHHFLHDGIEEEDEHDR"
     gene            1497..2786
                     /locus_tag="PKN79_00275"
     CDS             1497..2786
                     /locus_tag="PKN79_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ94240.1"
                     /translation="MTPLEKLAKAKRFDMKRKPIPQRHYLRPLTWLLSYPEVWLRRLK
                     IRRRGMEGIRPPFLLLCTHMAFIDFKVTTAALFPHRANYVVAIDGFIGREWLLRNAGG
                     ICKRKFTNDLQVVRQIKEVLHVNGDVLALYPEARYSLCGTTAVLPDSLGKICKLLGVP
                     VVMLNMHGHYLNSPVWNLKKRGVPIACDYFLIADAKETESLSVDELNERIRSAFEYDE
                     YRWQKENGIAITFKKRAEGLHKILYQCAHCHTEYEMHSFGSRIACKACKKEWEMGIYG
                     ELGASKPAEDETALVTEFPHIPDWYEWERSNVCREVEEGTYSLDVPVMIEALPNARGY
                     IPIGMGRLVHDMKGFTLETEGLFLHKDVSSMYSCHIEYEYKGKGDCIDLSTLDDTYYL
                     YPKEKPFAVTKVALATEELFFHAKKASSAEPDPIRPL"
     gene            complement(2752..3789)
                     /locus_tag="PKN79_00280"
     CDS             complement(2752..3789)
                     /locus_tag="PKN79_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNZ94241.1"
                     /translation="MSKKFVTFGEIMLRLKSPGHERFFQSPALEATFGGGEANVAVSL
                     SLLGKKAQFVTALPKNAIGEAAVREVRRYGVDTSAILRTEEGRVGIYFLETGANQRPS
                     LVVYDRAESSIATAKPSDFDFAKILKDAAWLHVTGITPAVSAEAAASTLAALKEAKKQ
                     GVTVSIDLNYRKKLWKWGKTAVEVMRELATYADVIVANEEDIQKCLGIEANVDVTSGE
                     LDTNVYKALTEEVKKQFPNISVIGVTLRESRSADVNGWSAAINGKTGFYLSRSYEITD
                     IIDRVGGGDSFSAGFIYALSEYGDDEEYVINFAVAASALKHSINGDFNLTTLSEVKAL
                     VKGDGSGRVQR"
     gene            3893..4855
                     /locus_tag="PKN79_00285"
     CDS             3893..4855
                     /locus_tag="PKN79_00285"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNZ94242.1"
                     /translation="MSDTLMKKIQLIGLVPVVKIDDAAKAVPLAKAMIAGGLPVAEVT
                     FRTAAAEEAISNIANAVPEMLLGAGTVTSVEFAKKAVKAGAKFIVSPGFNPEVVDWAL
                     ENNVPIVPGVCTPSDIEAGLSRDLSTLKFFPAEASGGVDMLKNFAGPFSNVMFMPTGG
                     INAKNLGSYAALSNVLAVGGSWMVKSDLIENEQWDEITALCAEAVLALQGLRFAHLGI
                     NETTKENAQVAAASLELLGMTRKVGNSSTFLDTVIEVTHAPFYGKHGHLGFACNNVDR
                     TVHYLQKKGFTLNEESVKRDDKGAMKSCYFNEEIAGFAFHLIKG"
     gene            complement(4852..6204)
                     /locus_tag="PKN79_00290"
     CDS             complement(4852..6204)
                     /locus_tag="PKN79_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HNZ94243.1"
                     /translation="MATIRATTLIDGGTYNNTERFQTLFDDLTEGDELVIPSGVYATG
                     ALTLKKHNVTITLEKGCRIQFIPDPELYPPVYTRWEGVNCWAMQPALLITDSSHIRLR
                     GEGMLDGSGSWWWQQAVVKKKQSKPITDLEHSFAHLNPGFEEQASGGGGRKSQFLRPP
                     LLQIHQSRNVHISGITLQNSPFWTLHPLYSKRVTIEDVTIFNPEDAPNTDGIDIDSCR
                     DVVVRNCIVDVGDDGIAIKSGSGRDGVVTNQSTVGVVIEGCTVRSAHGGAVIGSETAA
                     GVRDVLVQECIFDGTDRGIRIKTRRGRGGEITNIHYRSLTMKDNLCPIAVNMYYRPGS
                     TDPDLFSLDPQPILDTTPSISNILIEECTAVGATSSAGFIVGLPEEPIRNVRIVRSSF
                     GVSNENVTAIEESEMYDGLPAIRERGIRLRNVHLHLEDVKVLGVKEGFVVEEGVAFDS
                     "
     gene            6251..7120
                     /locus_tag="PKN79_00295"
     CDS             6251..7120
                     /locus_tag="PKN79_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ94244.1"
                     /translation="MMRFGVRAHDLGSFDTPEALAAAVRTFGEHLPVQLAVAKSFPSS
                     GPLDEAFALRIREAFDRQHVSIAVLGCYINPIHPDPAERERQLKRFEEHLALASILGA
                     PMVGTETGSVRPDCSYHPDTAEPKHLETLYSSLDRLVEYAVKVGSVVAVEAVAKQNTI
                     CSIERMARLAERFDTPRLKIIYDPVNLIPWIGIPEADGSFRARPSLEAQRAFTDSALD
                     AFGLRIAALHVKDFVLDEHGFKIGNLPALEGVFDWPALLADLRSRKISVPALLENANP
                     ATLESTLSTFYSL"
     gene            complement(7181..>7390)
                     /locus_tag="PKN79_00300"
     CDS             complement(7181..>7390)
                     /locus_tag="PKN79_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94245.1"
                     /translation="RRMVFKVEAWDEVEKIAEKALLYLFVEQNLIPEDLLLRIVDLLE
                     LDVAYMSKVLTDNKRPVSFAQPLLF"
BASE COUNT         1618 a         2171 c         1917 g         1684 t
ORIGIN      
        1 acttcaaaac ccatcaggga gtggcggggg tgggaacgtt cctcttcaac acctacatca
       61 atgacgattg gacgacgatc cattgtgaaa agggggatct tgcctactcc gtcctcgatc
      121 tgaatcagcc gattgttatc cgaacgaaag aaggaactga aaccatcgca ggcgatcctc
      181 cgccgaccca cgtcgcccag cccctcatcc ggacggtggt tgatgagctt ttgggaatcg
      241 gcacctgccc ttcgacgggc gagtccgccc tgcgaaccga ttgggttctt gccaggcttg
      301 ccggaacgat ctaacggtcg tgctcatcct cctcttcaat tccatcgtga aggaagtgat
      361 ggagcatgtc gtcgatcacc tcatcgagct cggcatcccg tttcccttca aagaccatca
      421 ctcccatatt gggcccgatc ttggtgagca gcggcaatgt tcgacggccg tagcagtgaa
      481 tcgcctgggg cttcacgcct cgatccacca ctgctagaaa gcgatcgatg aagatattgt
      541 gatccttctc atagtcatcg agcatataca catcgaggat ctgatgggct tggggatcat
      601 agagaagata gaagaccggg aacgtcggct catcaaattt ttcactggat acgggagcgg
      661 gaaagattcc cgtcgccatg tagaaaacct tcccgctttt cgctttcatc gatttatagt
      721 aacgccgctg ttcggcatcg gcgaactccg gtaccgggaa ggtgttttcg tagttccacg
      781 gttcaaagag gagcttgtcg agaactattt cattttcacc gaccgtcagg cggggaatat
      841 actcattctt ctttgaaccg tcggaaacct gatcatgcca catcgcgatc gcccggtgct
      901 tcggatgttc ttttttaatc tccgtcaggc ggtcgatgag gtggagagtc gccttgaaaa
      961 tccggataaa ctctttcgcc tcttcttcgt cgatgatccc gggaagacag tactccacct
     1021 tgcttcggaa gacgggagcc gccatatcct cgaagagaac gccgctctcc ttccgccacg
     1081 tctcatcggt ctcaagcatg tcgtcgtcct catcaaaagg aacgacgctg tacagcgaat
     1141 ccatcgcctg ctggtactcg aagaactcca gctcatcctc ggtctcctcg tgctcgaggt
     1201 actcgaggaa gttcgtcagt cccttcttgc cgcgattggc caccaacacc ccgtttccta
     1261 aggtcacgta ataggtcggt cgatttgcca gagaaaccgc aaacagatcc tcttcaaaga
     1321 agaggccgtc caggttggcg gtgaacgtcg cttttgcaag tgaatacagc tcggtaagaa
     1381 ggtctttatc catggttcca gtctagtgaa aatccatgag ttgtcaaatc atctttcccg
     1441 gcctcttgca atcccacggt tctaccccta ccatcgaacc catgaaggaa ggatgcatga
     1501 ccccgcttga aaaacttgcc aaggcgaaac gttttgacat gaaaagaaaa cccatacctc
     1561 agcgtcatta tctgcgtccg cttacctggc tgctcagcta ccctgaagta tggctaaggc
     1621 gcctgaagat caggcgaaga gggatggaag gcatcagacc gccgtttctt ctgctctgca
     1681 cccatatggc gttcatcgac ttcaaggtga cgaccgccgc cctcttcccc caccgggcga
     1741 actatgtggt cgcgattgac ggcttcatcg gccgcgaatg gctgttgaga aacgccggcg
     1801 gaatctgcaa acgcaaattc accaatgacc ttcaggtcgt gcggcagatc aaggaggttc
     1861 tgcatgtcaa cggcgatgtc ctcgccctct atccggaagc ccggtattcc ctctgtggaa
     1921 ccactgccgt cctccccgat tcgctgggga agatctgcaa gctgctgggt gttcccgtgg
     1981 tgatgctcaa catgcacggg cactacctca acagtcccgt ctggaatctg aaaaaacggg
     2041 gcgtcccgat cgcatgcgac tactttctga tcgccgatgc gaaagagacg gaatcacttt
     2101 cggtcgatga gctcaacgaa agaatccgca gtgccttcga atatgatgag tatcgctggc
     2161 agaaagagaa cgggattgcg atcaccttta aaaaacgggc ggaaggactg cataagatcc
     2221 tctaccaatg cgcccactgc catacggaat acgagatgca ttccttcggt tcccgcatcg
     2281 cgtgcaaggc atgcaaaaaa gagtgggaga tgggaattta cggcgaactc ggcgcctcga
     2341 aacccgctga agacgaaact gccttggtta ccgagtttcc ccatatcccc gattggtatg
     2401 agtgggagcg aagcaatgtc tgccgggagg tggaggaagg aacctattct cttgatgtgc
     2461 cggtgatgat cgaagcgctc cccaatgccc ggggatacat tcccatcggg atgggccggc
     2521 tcgtgcacga tatgaagggc ttcaccctgg aaaccgaagg gctgttcctc cataaggacg
     2581 tttcttcgat gtacagctgc cacatcgaat atgaatacaa gggaaagggc gattgcatcg
     2641 atctgtcgac cctggatgac acctactacc tctacccgaa agaaaagccc ttcgcggtga
     2701 ccaaggtcgc cttggccacg gaagagctct tttttcacgc caagaaagca tctagcgctg
     2761 aacccgaccc gatccgtccc ctttgacgag cgctttcacc tcgctcaagg tggtcaggtt
     2821 gaagtccccg ttgatcgagt gcttcaaagc tgacgcggcg accgcgaaat tgatcacata
     2881 ctcctcgtca tctccgtact cactcaaggc gtagatgaag cccgccgaga aggagtctcc
     2941 tccgccgacc cgatcaatga tgtcggtgat ctcgtaggaa cgggaaagat agaaaccggt
     3001 ctttccgttg atggcggcgc tccagccgtt cacatccgcg ctgcggcttt ccctcagggt
     3061 gacgccgatg acgctgatgt tcgggaactg ctttttcacc tcttcggtga gcgctttata
     3121 gacgttggta tccagctcac cggaagtaac atcgacgttc gcttcgattc ccagacactt
     3181 ctgaatgtcc tcttcattgg cgacgatcac atccgcgtag gttgcaagct cgcgcatcac
     3241 ttcaacggcg gtcttgcccc atttccacag cttcttgcgg tagttgagat cgatcgatac
     3301 cgtgactccc tgcttcttcg cttccttcaa tgccgccagg gtcgatgccg ccgcctcggc
     3361 gctgaccgcc ggagtgattc ccgtgacgtg gagccacgcc gcatccttaa ggatcttggc
     3421 aaaatcaaaa tcgctcggct ttgcggtcgc gatcgagctt tcggcgcggt catagacgac
     3481 gagactcggc cgttggttcg ccccggtctc aaggaaatag attcccactc ggccttcctc
     3541 ggttcggaga atcgccgaag tatccacccc gtagcgtcga acttccctca ccgccgcttc
     3601 tccgatcgca ttcttcggca aagcggttac aaactgagct tttttaccaa gcagggagag
     3661 cgatacggca acgtttgcct ctcccccacc gaacgtcgcc tccaaggccg gactctggaa
     3721 gaatcgttca tggcccggac ttttcaagcg aagcataatc tcaccaaatg ttacaaattt
     3781 tttactcaag gttttcctcc ttatctcttg ctattaccta ctatagagag tatagttctc
     3841 actgtcaatg tattttgaaa cactgtttca tatttaataa ggaggcattg taatgtctga
     3901 cactctgatg aaaaaaatcc aactcatcgg tctggtcccc gtcgtaaaga ttgatgacgc
     3961 cgccaaggcc gtacctctgg ccaaagcgat gatcgccgga gggctgcccg tagccgaagt
     4021 caccttcaga accgccgcag cggaagaagc gatctccaat atcgccaacg cggtgcccga
     4081 gatgctcctc ggagccggaa cggtaaccag cgtcgagttc gccaagaaag cggtcaaggc
     4141 cggagccaaa ttcattgttt cgcccggttt caaccccgag gtcgttgatt gggcgcttga
     4201 aaacaacgtt ccgatcgttc cgggtgtctg caccccgagc gatatcgaag cgggcttgag
     4261 ccgcgatctt tccaccctca agttcttccc cgccgaagcg agcggcggag tcgatatgct
     4321 caaaaacttc gccggtccct tctccaacgt gatgttcatg cccaccggcg gcatcaacgc
     4381 caagaatctc ggttcctacg ccgctctttc caacgtcctt gcggtcggcg gatcgtggat
     4441 ggtcaagagt gatctgattg aaaacgagca gtgggacgag atcaccgccc tctgtgcgga
     4501 agcggtgctc gccctccagg gtcttcgctt cgcccacctc ggaatcaatg agacaaccaa
     4561 agagaacgcc caagtcgccg cagcgtcgct tgagcttctg gggatgacca gaaaggtcgg
     4621 aaattcctcg acgttcctcg ataccgtcat tgaagtgacc cacgcgccgt tctacggaaa
     4681 gcacggacat ctcggctttg catgcaacaa cgtcgatcga accgtccact accttcaaaa
     4741 gaagggattc accctcaatg aggaatccgt gaagcgggat gacaagggag cgatgaagag
     4801 ctgctacttc aatgaagaga tcgccggctt cgcgttccac ctgatcaaag gctaagaatc
     4861 gaacgcaacc ccctcttcga cgacaaaccc ttccttcacc ccgaggacct ttacatcctc
     4921 gaggtgaagg tggacgtttc tgaggcggat tccccgctcg cggatggcag gaagtccatc
     4981 atacatctca ctctcctcga tcgccgtcac attctcattc gagacaccga aggaggagcg
     5041 cacgatccgg acgttgcgaa tcggctcctc gggaagaccg acaatgaagc cggcgctaga
     5101 ggtcgccccc acggcggtgc attcctcgat caggatgttg gaaatggacg gcgtggtatc
     5161 caatatcggc tgaggatcca agctgaagag gtcgggatcg gtggatcccg ggcggtaata
     5221 catgttcacc gcgatgggac agaggttgtc cttcatcgtg agggatcggt agtggatgtt
     5281 ggtgatctca cctccccggc cgcggcgggt cttgatccgg atgcctcgat cggttccgtc
     5341 aaagatacac tcttgaacaa gaacgtcccg caccccggct gcggtttcgc tgccgatcac
     5401 cgccccgcca tgggcgcttc gaacggtgca tccttcaatg acgaccccaa cggtcgattg
     5461 gttggtgacg acaccgtccc tcccgctgcc gctctttatg gcgatgccgt catcccccac
     5521 atcgacgatg cagtttctga ctacgacatc cctgcatgaa tcaatatcga ttccatcggt
     5581 attgggagcg tcttcgggat tgaagatcgt aacgtcctcg atcgttaccc gtttgctgta
     5641 gaggggatga agcgtccaga acggggagtt ttgcagagtg atccctgaga tgtggacgtt
     5701 cctgctctga tggatctgca agagaggcgg gcggagaaac tggcttttgc gaccgccacc
     5761 gccacttgcc tgctcctcaa agccgggatt caggtgagcg aaagagtgtt caagatcggt
     5821 aatcggtttg ctttgctttt tctttactac cgcttgctgc caccaccacg acccgcttcc
     5881 gtcgagcatc ccttcacccc gcaggcggat gtgagagctg tcggtaatca ataaggcggg
     5941 ctgcatcgcc caacagttca ccccttccca tcgggtatat acaggaggat aaagctcagg
     6001 gtcggggatg aactgaatcc ggcatccctt ttccaacgta atcgttacgt tgtgcttttt
     6061 gagcgtcagc gctccggtgg catagacacc ggaagggatg accagctcat cgccttcggt
     6121 taagtcatca aagagtgttt gaaaacgttc cgtgttgtta tatgttcccc cgtcaatcaa
     6181 cgttgttgcc cgtatcgtcg ccatcttctt ctttctttac tccctttcca aatcctttta
     6241 tactgcccgt atgatgagat tcggtgttcg tgcccatgat ctgggctcct ttgatactcc
     6301 cgaggcgctc gctgccgctg ttcgaacgtt cggcgaacac ctgccggtgc agctggccgt
     6361 cgccaaatcc tttccttcct ccggtcccct tgatgaagcg ttcgcgcttc ggatcaggga
     6421 agcgtttgac agacagcatg tttccatcgc agtgctcggc tgctatatca atccgattca
     6481 ccccgatccc gccgagcggg agcgccaact caagcgcttt gaagagcacc ttgcgctcgc
     6541 ttcgattctc ggagcgccga tggtcggaac ggaaaccggg tcggtccggc ccgactgctc
     6601 ctaccacccc gataccgctg aacccaaaca tcttgaaacg ctctactcct ccttggaccg
     6661 tctggtcgaa tacgccgtca aagtcggatc cgtcgtggcg gtcgaggcgg ttgccaaaca
     6721 gaacacgatc tgttcgatcg aacggatggc ccgcctcgcc gagcgcttcg ataccccccg
     6781 cctgaagatc atctacgacc cggtcaacct gattccctgg atcgggattc ccgaagccga
     6841 cggctccttt cgtgcaaggc cttcacttga agcccagcgc gccttcaccg acagcgccct
     6901 tgatgccttc ggcctgcgga tcgccgccct tcacgtcaag gacttcgtcc tcgacgaaca
     6961 cggcttcaag atcggaaacc tccccgctct tgaaggcgtc ttcgactggc cggcgctgct
     7021 tgccgatctg cgctctcgga agatctccgt ccccgctctt ctggagaatg cgaacccggc
     7081 aaccttggaa agcacgctct cgacgttcta ctcattgtaa gacacgaaaa gacccccctc
     7141 aatggactat tgcgtggggt ctttgcacgt tccagtactt tcagaagaga agcggctggg
     7201 cgaacgaaac cgggcgcttg ttgtcggtga gcaccttgct catgtaggcg acatccagct
     7261 caaggagatc gacgattcga agaagaagat cttcagggat gaggttttgt tcaacgaaga
     7321 gatagagcag tgctttctcg gcgattttct ccacctcgtc ccaggcttcg accttgaaga
     7381 ccatacggcg
//
LOCUS       DAOPUZ010000007         7229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_10310_length_7229_cov_3.650125, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000007 DAOPUZ010000000
VERSION     DAOPUZ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7229
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10310_length_7229_cov_3.650125"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /gene="rpmG"
                     /locus_tag="PKN79_00305"
     CDS             <1..168
                     /gene="rpmG"
                     /locus_tag="PKN79_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNZ94246.1"
                     /translation="DKKGPVEKIALQCTECKRRNYIIEKNRRNLPNKLEFSKYCPHDR
                     KHTLHRETKIK"
     gene            192..265
                     /locus_tag="PKN79_00310"
     tRNA            192..265
                     /locus_tag="PKN79_00310"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:226..228,aa:Trp,seq:cca)
     gene            304..483
                     /gene="secE"
                     /locus_tag="PKN79_00315"
     CDS             304..483
                     /gene="secE"
                     /locus_tag="PKN79_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNZ94247.1"
                     /translation="MKKIIKYFKESYLELKKVVWPSREAVISSTKVVLVSTILVAVFL
                     GLVDFLLLRGILFIL"
     gene            492..1052
                     /gene="nusG"
                     /locus_tag="PKN79_00320"
     CDS             492..1052
                     /gene="nusG"
                     /locus_tag="PKN79_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNZ94248.1"
                     /translation="MAKGWYVVQTYSGYEQKIERIINKMRESDTDFALVCTDVKVPME
                     TVIEIKDGQKREVKRKLLPGYILVELELPEHSWKTWVTKIRRIQGVSGFVTPSDRVKP
                     QPLSQAEEKSLFQKTGDLPAEQVFKPKQTFAVGEQVRIVDGPFESFVGTIEEVNLEKA
                     RMRVSVGIFGRSTPVEVDFLQVEKIP"
     gene            1212..1643
                     /gene="rplK"
                     /locus_tag="PKN79_00325"
     CDS             1212..1643
                     /gene="rplK"
                     /locus_tag="PKN79_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNZ94249.1"
                     /translation="MAKKKVTAIIKLQCPAQKATPAPPVGPALGPHGVSAPQFVQEFN
                     EKTKNYEPGLVLPVVITVYADRTFSFILKTPPAAVLIKRALGIAHGSGTPNKTKVGKL
                     TKDQVMEIAKTKMPDLNANDLEAAMKIVAGTARSMGVEVEK"
     gene            1643..1940
                     /locus_tag="PKN79_00330"
                     /pseudo
     CDS             1643..1940
                     /locus_tag="PKN79_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675888.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
     assembly_gap    1941..1950
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1951..2327
                     /locus_tag="PKN79_00335"
     CDS             <1951..2327
                     /locus_tag="PKN79_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNZ94250.1"
                     /translation="WMDFDVAVATPDMMKDVGRLGPILGRRGLMPNPKTQTVTNDITG
                     ALAELMKGRVEFRSDRTGVVHLAVGKVSMDPAHVKENTLEVVREVIRKKPADAKGDYL
                     ASVTLASTMGPGVRVDVRDLSL"
     gene            2340..2867
                     /gene="rplJ"
                     /locus_tag="PKN79_00340"
     CDS             2340..2867
                     /gene="rplJ"
                     /locus_tag="PKN79_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNZ94251.1"
                     /translation="MDYKTRITPAKEEAVQALMDEFKAYDGFIFADYRGMTVEQITKL
                     RRNLREKDAAFRVVKNRFAKIALTNMDMESDEHLTGPTAIALAKGDGANLVAKDLFNI
                     ARDGAPIKVKGALIDGMRFDAAQIEAFSKLPTRLELISSLMGTMKAPVQKLAATLLAY
                     VKEKGGDAVGAAKEE"
     gene            2935..3324
                     /gene="rplL"
                     /locus_tag="PKN79_00345"
     CDS             2935..3324
                     /gene="rplL"
                     /locus_tag="PKN79_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNZ94252.1"
                     /translation="MATIKKEEILEAIANMTVMEIAELIEMMEEKFGVQAAAAVAAGP
                     AAGAEVEAVEEPTEFNVILKAADPAKKIAAIKEVRAITGLGLKEAKDVVEAGGATIKE
                     SVSKADADALKEKLEAAGCTVEIVPVD"
     gene            3471..6983
                     /gene="rpoB"
                     /locus_tag="PKN79_00350"
     CDS             3471..6983
                     /gene="rpoB"
                     /locus_tag="PKN79_00350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNZ94253.1"
                     /translation="MVANGKSITRTYIGTESREVCELPNLIGVQLESYERFLQLETIR
                     KGLEPDPDLGLENVFRSTFPIDSPNGEMRLTYRGYTIDYDNVKFTETECKKKGRSYSV
                     PIKVTISLEFFATGEMREKEIFFGDIPLMTDRGTFIINGAERVVVSQIHRSPGVIFSH
                     DKDVYSSRIIPYRGSWLEFEIDEKKSLIYAKIDRKKKILGTLLLRAIGYDSREKIIDA
                     FYKSVDVELIDDPEMRTTLENRYLAKDVYAMINGEERKVLRAGDLLHAHEIDELFKLG
                     VNLIQLVDLKGEGSLHSEIILNCFEIEDAKYTREGFDEPTKEDVLTPIFSVLMPGEMI
                     AIERAEKDLPDMFFSPRKYDLGSVGRYKFNKKFGEELEDNDAVADESTVLTPEDITNT
                     MGYLIKVFIHEANVDDIDHLGNRRVRSVGELLQNALRGAFARMERIAKERMNLETGSV
                     KPQDLISIKPIVAAIKEFFGSSQLSQFMDQVNPLAELTHKRRLNALGPGGLSRDRAGF
                     EVRDVHYTHYGRICPIETPEGPNIGLIVSLANYTRINRHGFLETPYRRVVDGKVTDQY
                     RFLDANDEEKYYIAQASAAFDEDGQFLDKEVPVRHSGDYSTRAPEEIRYMDISPKQVI
                     SVAASLIPFLEHDDANRALMGSNMQRQAVPLVFPETPRVGTGMEGKTAYDSGVLVKAH
                     RSGVVSYVSSDKIIVIPTSSEIEGEMDVYEVQKYERTNQDTCFNQKPIVEVGQRVEVD
                     QVLADGPATQHGELALGRNILVGFVPWHGYNYEDAVLISERVVKDDIFTSVHIKEFTT
                     DIRETKLGPEKLTRDIPNTSEKMLEQLDADGIVRIGATVRPGSILVGKVTPKSESDTT
                     PEFKLLNSIFGEKAKEVRDTSLKVPHGTEGTIIDIQRLKRSNNDELPPGVEETVKVLI
                     ATKRKLRQGDKMAGRHGNKGVVSKILPEEDMPFMEDGTPLDVCLNPLGVPSRMNIGQL
                     METQLGWAAVALDEWYSTPVFQSASMEQLEEKMREAGLPEDSKAILYDGQTGVPFVNP
                     VFCGYIYYLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYG
                     AANTLQELLTIKSDDMNGRVKIYESIVKGEPATTAGMPEAFNVLVQEIRGLALDMSVY
                     DADGNLVPLTERDEELLAKQGSAN"
     gene            6995..>7229
                     /locus_tag="PKN79_00355"
     CDS             6995..>7229
                     /locus_tag="PKN79_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94254.1"
                     /translation="MREIQEFDSIMIKLASPEQIKGWSYGEVKKPETINYRTLRPERD
                     GLFCERIFGTTKEWECHCGKFKSIRYKGVICDRC"
BASE COUNT         1804 a         1893 c         2056 g         1466 t
ORIGIN      
        1 gataaaaaag gtcctgttga gaagatcgct ctccagtgca ccgaatgcaa acggagaaac
       61 tatatcatcg agaaaaatcg gcggaacctc ccgaacaaat tggagttcag caagtattgt
      121 ccgcatgacc gcaagcacac cttgcaccgc gagacgaaga ttaaataggt tatagcgatc
      181 gagaagctaa taggccaata gctccaattg gtagagcgcc ggtctccaaa accggatgtt
      241 gagggttcga atccttcttg gcctgttcgc ttctcgatcc tttgaataca aggagcctgt
      301 ctcatgaaga aaatcatcaa gtattttaag gaatcatatc ttgaactgaa gaaggtcgtc
      361 tggccctccc gggaagcggt gatttcttct accaaggttg tcctcgtttc cacgattctt
      421 gtcgctgtct tcctgggttt ggtggatttc cttctcttga gaggaattct cttcatctta
      481 tagggttgag catggcaaaa ggctggtatg tagtacagac ctattccggg tacgagcaaa
      541 agattgagcg gatcatcaac aagatgcgcg agtccgatac ggatttcgcc cttgtctgca
      601 ccgatgtgaa ggtgccgatg gaaacggtca ttgaaataaa ggacggacag aaacgcgaag
      661 tgaagcgaaa gcttcttccc ggatatattc ttgtcgagct cgagttgccg gagcacagct
      721 ggaagacgtg ggttaccaag attcgccgaa ttcagggtgt ttcagggttt gttactcctt
      781 ccgatcgggt caagccccaa ccgctttcac aggcggagga gaagagcctc tttcagaaga
      841 ccggcgacct tcccgccgaa caggtgttca agcccaaaca gacgttcgct gtcggcgaac
      901 aggtcaggat tgtcgacggc cccttcgaat cctttgtcgg aacgatcgag gaggtcaacc
      961 tcgagaaagc gcgaatgcgc gtctcggtgg gaattttcgg ccgatccact ccggttgaag
     1021 tggacttttt acaagtagaa aagattccgt aagattcgtg gacaggatct tacacgagag
     1081 ttttctgatt tttaacacgt gtccatgtaa aaatgcaaac tccctcgtta tgaggtggga
     1141 gcctgtcgtt gacaggcgtt aataccagcg caagacgcca tacaggcgag cttgtgtaag
     1201 gagaaaggaa catggcaaag aagaaggtga ctgcaatcat caaattgcag tgccctgccc
     1261 agaaggccac ccccgcgccc cctgtcggcc cggcgctcgg ccctcatggc gtcagtgccc
     1321 ctcagtttgt ccaggagttc aacgaaaaga cgaagaacta cgaacccgga cttgtgcttc
     1381 ccgttgtcat taccgtgtat gccgatagga ccttcagttt catcctgaag actcctcccg
     1441 ctgccgtcct gatcaagagg gcattgggaa tcgcacatgg tagcggtact cccaacaaga
     1501 ccaaagtcgg taaactgacg aaggatcagg tgatggagat cgccaagacc aaaatgccgg
     1561 acttgaacgc gaacgacctt gaagcagcga tgaagatcgt tgcgggaaca gctcgcagca
     1621 tgggtgtgga ggttgagaag taatgaaaca cggaaagaaa tatcgtgcat ccgccgagaa
     1681 agtcgatcga acgaagtttt atacgttgga tgaggccacc gcgctcgtaa aagaggcggc
     1741 gttcgccaag ttcgacgaga cggtcgaggt gcatatcaaa ctgactctca agaagagtca
     1801 gagtgttcgc gacacggtcg tcctgcccca tcagttcagt gctcagaagc gtgttctcgt
     1861 ctttgcaaaa ggcgagaaag cggatgaagc gcgggctgcg ggagcgacct acgtcggtga
     1921 tgatgacctg attgaacaga nnnnnnnnnn gttggatgga ttttgatgtg gcggttgcta
     1981 ctccggacat gatgaaggat gtcggccgac tcgggccgat cctcggtcgc cggggcttga
     2041 tgcccaaccc caaaactcag accgttacca acgatatcac cggcgctctc gccgagttga
     2101 tgaaaggtcg agtcgagttc cgctctgatc gaaccggggt tgtccacctc gccgtcggca
     2161 aggtttcgat ggatcccgcc catgtcaagg agaacacgct tgaagtggtg cgcgaagtga
     2221 tccgcaaaaa gcccgccgat gcaaaaggcg actacctggc cagcgtaacc ctcgcttcga
     2281 cgatgggtcc gggtgttcgc gtcgacgtac gtgacctctc actctaagga ggaatgaaga
     2341 tggattacaa aactcgaatt acccccgcca aggaagaggc tgttcaggct ttgatggatg
     2401 agttcaaagc gtacgatggt ttcatcttcg ccgattaccg cgggatgacg gttgaacaga
     2461 tcaccaagct gcgcaggaac ctccgcgaga aagatgcggc gttccgcgtg gtgaagaatc
     2521 gcttcgccaa gatcgctttg accaacatgg acatggaaag cgatgagcac ctgacgggtc
     2581 ccacggcgat cgccctcgcc aagggtgatg gagcgaacct tgtcgccaag gatctcttca
     2641 acatcgcccg ggacggagct ccgatcaaag taaagggcgc cttgatcgac ggaatgcgct
     2701 ttgacgcggc tcagattgag gcgttcagca agctgccgac caggctcgaa ctgatttcct
     2761 cgctgatggg cacgatgaaa gcgccggttc aaaagttggc ggctacgctg cttgcctatg
     2821 tcaaagagaa gggtggtgac gccgtcggcg cagccaaaga agaataaacc acagtcttag
     2881 tcttcactca gtacctgcta cgactgtgag agacaatctt aaggagaaga taatatggct
     2941 actattaaaa aagaagaaat tttggaagcg atcgcgaaca tgaccgtgat ggaaatcgcc
     3001 gagcttatcg agatgatgga agagaagttc ggcgttcagg ctgcggccgc tgttgcagca
     3061 ggcccggcag ccggtgcgga agttgaagcg gttgaagagc cgaccgagtt caacgtcatt
     3121 ctcaaagcag ccgatcctgc caagaagata gcggcgatca aggaagtccg ggcgatcacc
     3181 ggtcttggcc tcaaggaagc caaggatgtt gtcgaagccg gaggagccac gatcaaggaa
     3241 agcgtaagca aggcggatgc cgacgcgctg aaggagaagc tcgaagcggc cggatgtacc
     3301 gtcgagatcg ttcccgtcga ctaaggataa gatccttgag aacaattcgc gaagcgtgcg
     3361 aaatgttgaa ttctcaacgc caccggaatc ccccggtggc atgttgtgtc ctcatctcct
     3421 cccgcggggg gatttgagtc gcttgttcat ttgactttgg agggtacaca atggttgcca
     3481 acggcaagtc cataactcgg acctacatcg gtactgaatc acgtgaagtg tgcgaactgc
     3541 ccaatctcat aggggtgcag ctggaatcct atgaacggtt tttgcagctg gagacgattc
     3601 gcaagggctt ggagcccgat ccggatttgg gtcttgagaa tgtatttcga tccacattcc
     3661 cgatcgacag ccccaacggc gagatgcgtt tgacgtatcg cggctatacg atcgattatg
     3721 ataatgtaaa gttcacggaa acggagtgta agaagaaggg ccgctcctac agcgttccca
     3781 tcaaagtcac gatcagtctt gaattttttg ctaccggaga gatgcgcgaa aaagagattt
     3841 tctttggcga catccctctg atgaccgacc gcggcacctt catcatcaac ggggccgaac
     3901 gggttgtggt cagccagatc caccgctccc ccggtgtgat cttcagtcat gacaaggacg
     3961 tttattcatc ccgcatcatc ccatatcggg gatcttggct ggaatttgaa atcgatgaaa
     4021 agaaatcgtt gatctatgca aaaatcgacc gaaagaagaa gattctcgga accttgctgc
     4081 ttcgggcgat cggctacgac agccgcgaaa agatcatcga tgcattctat aagagtgtgg
     4141 atgtcgaatt gatcgacgat cccgaaatga ggacgacgct ggagaaccgt tatcttgcca
     4201 aggatgtcta tgcgatgatc aacggcgaag agcggaaggt gcttcgagca ggagatctct
     4261 tgcatgccca cgaaatcgac gagctcttta agctcggtgt gaatctcatt cagctcgtag
     4321 acttgaaagg ggaggggtcg cttcatagtg aaatcatcct caactgtttt gagattgaag
     4381 atgcgaagta cacccgagaa gggtttgatg agccgacgaa ggaagatgtc ctgaccccga
     4441 ttttcagcgt cctgatgccc ggcgagatga tcgccatcga gcgggccgaa aaagatctgc
     4501 ccgatatgtt cttctccccc cgaaagtatg atctcggctc cgtcggccgg tacaagttca
     4561 ataaaaaatt cggggaggag ctggaagata atgacgccgt cgccgacgag tcgaccgtcc
     4621 ttacccccga agacatcacc aacacgatgg gctacctcat caaagtgttc atccacgaag
     4681 ccaacgtcga cgatatcgac cacctcggaa accgccgggt gcggagcgtc ggcgaactcc
     4741 tccagaacgc cctgcgcggc gcgttcgccc ggatggagcg aatcgcaaaa gagcggatga
     4801 accttgaaac cggatcggtc aagccgcagg acctgatctc gatcaagccg atcgtcgccg
     4861 cgatcaagga gttcttcggc tcttcccagc tttcacagtt catggaccag gtgaaccctc
     4921 ttgccgagct gacccacaag cgccgcctga acgccctcgg tcccggcggc ctgtcccgtg
     4981 accgcgccgg cttcgaggtc cgcgacgtcc actacaccca ctatgggaga atctgtccga
     5041 tcgagacccc cgaggggccg aacatcggct tgatcgtctc cctggcaaac tatacccgaa
     5101 tcaaccgcca cggttttctt gagacgccct atcgacgggt cgtcgacgga aaggtcaccg
     5161 accagtaccg tttcctcgat gccaacgatg aagaaaaata ctacatcgcc caggcgagcg
     5221 ccgcttttga cgaggatggc cagttcctcg ataaggaagt gccggtgcgc catagcggtg
     5281 actactccac ccgggctccc gaagagattc ggtatatgga catctccccg aagcaggtga
     5341 tcagcgtcgc cgcttccctg attccgtttc tggagcacga cgacgccaac cgtgcgctga
     5401 tgggttcgaa catgcagcgc caggcggtcc cgctggtgtt ccccgagacc ccgcgggtcg
     5461 gaacgggaat ggaaggaaaa accgcctatg attccggcgt gcttgtcaaa gcgcaccggt
     5521 cgggcgttgt aagctacgtg tccagcgata agatcatcgt catcccgacc tcctcggaga
     5581 ttgaaggcga gatggatgtc tatgaggtgc agaagtatga gcgcaccaat caggatacct
     5641 gcttcaatca gaagccgatc gtcgaagtcg gtcaacgcgt tgaagtggat caagtgctcg
     5701 ccgacggacc ggcgacccag cacggcgaac tcgccttggg acgcaacatc cttgtcggct
     5761 ttgttccctg gcacgggtac aactacgagg acgccgtgct catcagtgaa cgggtcgtca
     5821 aagacgacat cttcaccagc gtccacatta aggaattcac caccgatatc cgtgagacga
     5881 agctgggacc ggagaagctg acccgcgata ttccgaatac gagcgagaag atgctggagc
     5941 agcttgacgc cgacggaatt gtccgaatcg gcgcaacggt tcgccccggt tcgatcctcg
     6001 tcgggaaagt cacccccaag agcgagagcg acacgactcc cgagttcaag ctgctcaact
     6061 cgatcttcgg tgaaaaagcg aaggaagtcc gggatacatc gctgaaggtc ccccacggaa
     6121 ccgagggaac gatcatcgac atccagcggc tcaagcggag caacaacgat gagcttcccc
     6181 ccggggtcga agagacggtg aaggtcctga tcgccacgaa gaggaagctt cgccaaggcg
     6241 acaagatggc cggtcgacac ggaaacaagg gcgtcgtttc aaaaattctt cccgaagagg
     6301 acatgccctt catggaagac ggaactccct tggatgtgtg tctcaacccc ctcggtgttc
     6361 cgtcccgaat gaatatcgga cagctgatgg aaacccagct cggatgggcg gccgtcgctc
     6421 tcgacgagtg gtattccacc ccggtgttcc aaagcgcatc gatggagcag cttgaggaga
     6481 agatgaggga ggcgggtctt cccgaggatt caaaggcgat cctctatgac gggcagaccg
     6541 gagttccctt cgtaaacccc gtcttctgcg gctacatcta ctatctgaag ctgcaccacc
     6601 tcgtcgacga caagatgcac gcaaggtcca ccggtcccta ctcgctggtg acccagcagc
     6661 cgctcggcgg taaagcccag ttcggcggtc agcgcctcgg agagatggaa gtatgggccc
     6721 tcgaagcata tggtgctgcg aacaccctcc aggagctgct cacgatcaag agtgacgata
     6781 tgaacggacg ggtgaagatc tacgaatcga tcgtcaaggg cgagccggcg accacagcgg
     6841 gaatgcccga ggcattcaac gtgctggttc aagagattcg gggccttgct ttggatatgt
     6901 cggtatatga tgccgacggc aatctggtgc cccttaccga acgtgacgaa gaattgctgg
     6961 caaagcaagg ctcggcgaat taaggagaga agtgatgaga gagattcaag aattcgattc
     7021 catcatgatc aagctcgcct ccccggaaca gatcaaaggc tggtcgtacg gtgaggtaaa
     7081 gaagcctgaa acgatcaact atcgcacgct gagaccggag cgtgacggtc tcttctgcga
     7141 gcggatcttc ggaacgacga aggagtggga gtgccattgc ggaaagttca agtcgatccg
     7201 ctacaagggc gtgatctgtg accgctgca
//
LOCUS       DAOPUZ010000008         7031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_10688_length_7031_cov_3.085149, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000008 DAOPUZ010000000
VERSION     DAOPUZ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10688_length_7031_cov_3.085149"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /gene="leuS"
                     /locus_tag="PKN79_00360"
     CDS             complement(<1..1620)
                     /gene="leuS"
                     /locus_tag="PKN79_00360"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNZ94255.1"
                     /translation="MSKYPFSAIEKKWQAYWEENQSFAVTEDPTVPEEKRLYVLDMFP
                     YPSGSGLHVGHPEGYTATDIYCRYLRMNGYNVLHPMGFDSFGLPAENYAIKTGTHPKV
                     TTEHNIENFRRQIKSLGFSYDWSREISTHESEYYRWTQWIFLQLYKKGLAYEAHTPIN
                     WCDNCKTGLANEEVKDGKCERCGTPVVRKTIRQWILRITEYAERLLAGLDTLDWPESV
                     KLMQRNWIGRSEGATVRFRIEGMDESLEVFTTRPDTIFGATYMVVSPEHPLVGELTTV
                     AQRPAVERYLDETSHKSDLERTDLAKEKTGVFSGSYAINPVNDERIPIWIADYVLISY
                     GTGAIMAVPAHDTRDWEFAKKFNLPIIEVLKSEIDVQEEAWVEDGLHVNSGFIDGLDK
                     HDAIEKMIAFLEERKLGERAVNYKLRDWIFSRQRYWGEPIPLLHCEKCGTVPVPEDQL
                     PLLLPEVTSYEPSGTGESPLAKIDSWVNTACPVCGGAARRETNTMPQWAGSCWYHLRY
                     LDPGNTERFTGEDAEKYWMPVDLYVGGAEHAV"
     gene            complement(1649..2902)
                     /locus_tag="PKN79_00365"
     CDS             complement(1649..2902)
                     /locus_tag="PKN79_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNZ94256.1"
                     /translation="MNISLAVVFALLVLSALFSATETAYTSLSFFQLKTLENRKSRAG
                     KLAYALSQDKDHLITTVLIGNNIVNLSASALVTTITIKYFSSALVGYTTGILTFMILV
                     FGEIVPKRLALVHNVRIAILMAYPIKTLMLLLFPLAWLLQVLSSAITRLFGTKEEPVI
                     TTEGVMHVVDAAEDVGLVDQYESDLMQRAIHFSSTTVRTIMTHRTEVFTLPSDLTIEE
                     AFPKIIKSGFSRIPIYQDNQENIVGILLLRDVLRAQQKQVVNKTLASLSRKPIYVVEQ
                     MHLDDLFYLFKKNKLQQAIVIDEYGGFSGVVTMEDVAEQLFGELFDEHESRFPDRVVQ
                     HKDKPGTFVVMADAPFQQVVDDLDLWYEGDRVSTVAAYLLQEAGSIPVEGEVITTDLG
                     TWQILLMKGNKVEVVEFTPNPASVD"
     gene            3121..3378
                     /locus_tag="PKN79_00370"
     CDS             3121..3378
                     /locus_tag="PKN79_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94257.1"
                     /translation="MKDNNTVICSFSMDRNLYNAYKNIVTQNGENVKDNLVRYMQQVV
                     DYEIPNAETIKALEEVKRLKADPHTKTYGSFAEIMEELYDA"
     gene            3414..3608
                     /locus_tag="PKN79_00375"
     CDS             3414..3608
                     /locus_tag="PKN79_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HNZ94258.1"
                     /translation="MSKKPSGEPLPKKYRDHGLSGNDKGCRECHITPDWLLIYEISGS
                     ELILYLTRTGTHSICFSGNP"
     gene            3674..5476
                     /locus_tag="PKN79_00380"
     CDS             3674..5476
                     /locus_tag="PKN79_00380"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNZ94259.1"
                     /translation="MGATLYDESKILTLSALEHIRMRPGMYIGRLGNGSHIDDGIYIL
                     IKEVIDNSIDEFIMGHGSKIVIRLKDNRVRIRDWGRGIPLGKVIECVSEINTGAKYND
                     DVFQFSVGLNGVGTKAVNALSSDFEVTSFRDGKFTNAVFKQGILVKETSGKSDEANGT
                     EVIFTPDEELFGDYTFNEDFIIARIWGYAYLNTGLSLEFNGRTFRSSKGLGDLLAKEV
                     ADEGLYTTIHHKGERLEFAMTHVSGTYGESYFSYVNGQHTSDGGTHLSAFKEGIGKGI
                     SEYFKKNWSPQDVREGIIGAIAVKIKSPIFESQTKNKLSNTEIRTWIVNEVKDAIIDF
                     LLKNPKDAEKISIKVMQNEALRKELNEVKKGARESARKVSINIPKLKDCKYHLGQSAA
                     HKEKCDNSMIFITEGDSASGTITKTRDVLTQAVFSLRGKILNVHGKKKTEIYKNAELY
                     NLMVALGIENGIEDLRYGKVIIATDADTDGYHIRNLLMTYFLTYFEDLVLSGRLYILE
                     TPLYRVRNRTTTEYAYNEKQRDDAVARLRGAEVTRFKGLGEIDPREFGQFIGEDMRLI
                     RVSLPGVTEMHRSLEFYMGSNTPERRSFIMENLI"
     gene            5476..>7031
                     /locus_tag="PKN79_00385"
     CDS             5476..>7031
                     /locus_tag="PKN79_00385"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNZ94260.1"
                     /translation="MTQAHQLFRQNFLEYASYVIKERAIPDLVDGFKPVQRRIIHTLF
                     EMDDGKFHKVANTVGWAMRFHPHGDASIYEALVNLANNDLFIDRQGNFGNILTGDSAA
                     APRYIVCRLLLVAKEVLYNPELTEWVESYDGRNKEPVVFPAKIPVVLIQGVMGIAVGM
                     STLILPHNRFEVLDAMAAHLRGESFELYPDFPQGGIVDVEDYNDGKGTVLVRARLNTS
                     DPKRIIVEELPYGTTSESLINSIEDAAKKGRLKISSINDFTAESVNIEINLARNTYSK
                     DIVDALYAYTDCEKRITTNPLVIRDNVPTVMGVSEILAFHAEHLKVVLKAELELEEEK
                     LLAKMHDRTLERIFIEERIYKRIETKRSADDVLKAVHTGLKPFSSELLRDVTDEDVER
                     LLKIPIRRISLFDIERNRQEIEQINEQLEEVRSKLENLTGYALETIDRLKGMFDRKEH
                     KRRSTLKKFELVDVKEVAVRDLVVRYDSTNGYLGYGVKTGAEQVTVSQFDRLLVIRRD
                     GSYQVIDAPE"
BASE COUNT         1677 a         1886 c         2000 g         1468 t
ORIGIN      
        1 taccgcatgc tccgctccgc cgacgtacaa gtcgacgggc atccagtact tttccgcgtc
       61 ttctccggtg aagcgctcgg tgttgccagg atcgagataa cgcaggtggt accagcagga
      121 ccctgcccat tggggcatcg tgttggtctc ccgtcttgcc gctccgccgc atacgggaca
      181 cgcggtgttc acccaggaat cgatcttcgc caaagggctt tcgccggttc ccgagggctc
      241 atagctcgtc acttcgggaa gaaggagggg aagctgatcc tccggtacgg ggacggttcc
      301 gcacttctcg cagtggagca gggggatcgg ttcgccccag tagcgctggc ggctgaagat
      361 ccagtcccgc agcttgtagt tgaccgcccg ctcccccagc ttccgctctt cgaggaaggc
      421 gatcatcttc tcgatcgcat catgtttgtc caacccgtcg atgaacccgc tgttgacgtg
      481 gaggccgtct tcgacccagg cttcttcctg cacgtcgatc tcgcttttca gcacttcgat
      541 gatcgggagg ttgaacttct tggcaaactc ccagtcgcgg gtgtcgtggg cgggaaccgc
      601 catgatcgct ccggtgccgt agctgatcag gacgtagtcg gcgatccaga tcggaatccg
      661 ctcgtcgttc accgggttga tcgcgtacga tccgctgaag acccccgtct tctcctttgc
      721 cagatcggtt cgctcaaggt cgcttttatg ggaggtttca tccaaatacc gctcaacggc
      781 cgggcgttgt gccactgtag tcaattcgcc gaccagcggg tgctcggggg agacgaccat
      841 gtaggtggcg ccgaagatgg tatcgggtcg ggtggtgaac acctcgaggc tttcatccat
      901 cccctcgatc cggaaacgaa ccgtggcgcc ttcgctccgc ccgatccagt tgcgctgcat
      961 cagcttcacc gactcgggcc aatcgagggt gtcgagcccg gcaagaaggc gctcggcgta
     1021 ctcggtgatt ctcagaatcc attggcggat cgtcttccgg acgaccgggg ttccgcagcg
     1081 ctcgcacttt ccgtctttga cttcctcatt ggcaaggccg gtcttgcagt tgtcgcacca
     1141 gttgatcggg gtatgggctt cgtacgccag tcctttttta tagagctgca ggaagatcca
     1201 ctgcgtccag cggtagtatt cgctttcatg ggtcgaaatc tcccggctcc agtcgtagct
     1261 gaagccgagg ctcttgatct ggcggcggaa gttttcgatg ttgtgctcgg tcgtgacctt
     1321 cgggtgggtt cccgtcttga tcgcatagtt ttccgccgga agcccgaagg agtcgaagcc
     1381 catcgggtgg aggacattat acccgttcat cctgaggtat cgacaataga tgtcggtggc
     1441 ggtgtatcct tcggggtgtc cgacgtggag cccgcttccc gaggggtagg ggaacatgtc
     1501 gagcacgtag aggcgcttct cttcgggaac ggtgggatct tcggttacgg caaagctctg
     1561 gttctcttcc cagtacgcct gccacttctt ttcaatggcg gaaaacgggt atttgctcat
     1621 gtgagcaatc atagcgttca cgttttgttc agtcaacgct cgccgggttc ggggtgaact
     1681 ccacgacctc cactttattg cctttcatca gaaggatctg ccacgttccg agatcggtgg
     1741 tgatcacctc cccttcgacg ggaatcgagc ccgcctcctg caagagatat gccgccacgg
     1801 tgctgacccg atccccttca taccacaagt ccagatcatc gaccacctgc tgaaagggag
     1861 catccgccat gacgacgaac gtgccgggct tgtccttgtg ctggacgacc cggtcgggga
     1921 accgcgattc atgctcgtcg aagagctcgc caaagagttg ttcggcgaca tcctccatcg
     1981 tgacgacacc ggagaacccg ccgtactcgt cgatgacgat cgcctgctgg agcttgtttt
     2041 tcttgaagag gtagaagagg tcgtcaaggt gcatctgctc gacgacgtag atcggttttc
     2101 ggctcagcga agcgagggtc ttgttgacga cctgtttctg ctgggcgcga agcacatccc
     2161 gcaaaaggag gattccgacg atattctcct ggttgtcctg atagatcgga atccggctga
     2221 agcccgattt gatgatcttg ggaaacgctt cttcgatcgt caggtcggag gggagggtaa
     2281 agacttcggt ccggtgagtc atgatcgtcc ggacggtcgt cgaggagaag tggatcgctc
     2341 gctgcatcag gtcggattca tactggtcga ccagccccac atcctcggcg gcgtcgacga
     2401 cgtgcatcac cccttcggtg gtgatgaccg gttcctcctt ggttccgaac aggcgggtta
     2461 tcgcactgga aaggacttgg agcagccatg ccaggggaaa aagcagaagc atcagggtct
     2521 ttatcggata agccatgagg attgcgatgc gcacattgtg caccaaagca agccgtttgg
     2581 gcacgatttc accgaaaacg agaatcatga aggtaagaat cccggtggta tagcctacca
     2641 aggcggagga gaaatacttg atggtgatcg tcgtaaccag agccgaagcg ctgaggttca
     2701 cgatgttgtt tccgatgaga accgtagtga tcaggtgatc cttgtcctga ctcaacgcat
     2761 atgccagctt cccggcccgg gattttcgat tttccaaggt cttgagctga aagaaggaaa
     2821 gggaggtgta ggcggtttcc gttgcgctga agagcgccga aagaacaaga agggcaaaaa
     2881 cgacagcaag ggagatgttc atgcacacct ccacaggcaa gatctaggta acgggtcttc
     2941 cataatgggc tcagtatatc aactcaaggc gggtgaggac aaggtaaaag aagggatccg
     3001 atcaagaagc ggacagggat ggtgggggtg gattaacaac caagtgtaca accctccgaa
     3061 atattcatat tctatcattg ctaagtatgg ttactaagta aatggaaagg agccatggcc
     3121 atgaaggaca acaatacggt aatctgttct ttttcaatgg acagaaacct ttacaatgct
     3181 tataagaaca ttgtcactca aaatggggaa aacgtaaaag acaatctcgt acggtatatg
     3241 caacaggtgg ttgattatga aataccaaat gcggaaacca tcaaggcgct tgaagaagta
     3301 aagagattga aagcggatcc ccatacaaaa acgtatgggt cttttgcgga gatcatggaa
     3361 gaactttacg atgcctaaat acaggatcgt tcaaaccgga gtatgtaaaa aggatctcaa
     3421 aaaagccatc gggagagcct ttaccgaaaa aatacaggga tcacggctta tccggaaatg
     3481 ataaaggatg tagagaatgc cacataaccc cggactggct tctcatctat gagatatcag
     3541 gcagtgaatt gattttatac ttgactcgaa cgggcacaca ctcgatctgt tttagcggta
     3601 acccatgatt atgcaataaa cccatgtata cgacactttt gccaaccccg gacctttctg
     3661 ctatactggg gtcatgggtg caacattata tgatgaatca aagatcctga cgctcagtgc
     3721 gcttgagcat attcggatgc gtccgggaat gtatatcgga cgcttgggaa acggatccca
     3781 catcgacgac ggaatctata tcctcatcaa ggaagtcatc gacaactcaa tcgacgagtt
     3841 catcatggga catggctcga agatcgtgat ccgcctcaag gacaaccggg tgaggatccg
     3901 cgactggggc cgggggattc ctctgggcaa agtgatcgag tgcgtctccg agatcaacac
     3961 cggagccaag tacaacgacg atgtcttcca gttctccgtc ggattgaacg gagtcggaac
     4021 caaggcggtc aacgcgctct cctccgactt cgaagtcacc agcttccggg acggaaagtt
     4081 caccaacgcg gtcttcaaac agggaatcct tgtcaaggag acttccggca aaagcgatga
     4141 ggccaatggg acggaagtga tcttcacccc ggacgaagag ctcttcggtg actatacatt
     4201 caatgaagat ttcatcatcg cccggatctg gggttacgcc tacctgaaca ccggcctgag
     4261 tctggagttc aacggcagga cgttccgctc ctcaaaaggc ctgggcgacc ttctggcgaa
     4321 agaggtcgct gacgaaggcc tctataccac gatccatcac aagggcgaac gcctggagtt
     4381 cgccatgacc cacgtcagcg gaacctacgg ggaaagctat ttctcctacg tcaacggtca
     4441 gcacaccagt gacgggggaa cccacctttc ggcattcaag gaagggatag ggaaggggat
     4501 cagcgagtac ttcaagaaga actggtcgcc tcaggatgtc cgtgaaggga tcatcggagc
     4561 gatcgcggta aagatcaaaa gcccgatttt cgagtcgcaa acgaagaaca agctttccaa
     4621 cacggagatc cgcacctgga tcgtcaacga ggtgaaagac gcgatcatcg atttcctgct
     4681 gaagaacccc aaggatgccg agaagatcag cataaaggtg atgcagaacg aagcgctcag
     4741 aaaagagctg aacgaagtga agaaaggggc ccgggagtcc gcccggaaag tctcgatcaa
     4801 catccccaag ctgaaggact gcaagtatca cctgggccaa agcgccgccc acaaggagaa
     4861 gtgcgacaat tcgatgatct tcatcaccga aggcgattcg gcgagcggaa cgatcaccaa
     4921 gacccgcgac gttttgacac aggcggtgtt cagcctcagg ggaaagattc tcaacgtcca
     4981 cggaaagaag aagaccgaga tctacaagaa cgccgagctg tacaatctga tggtcgccct
     5041 cggcatcgag aacgggatcg aggatcttcg ctacgggaag gtcatcatcg ccaccgacgc
     5101 cgacacggac gggtatcaca tccgcaacct gttgatgacc tatttcctca cgtactttga
     5161 agacctcgtt ctttcggggc gcctgtatat cctggagacc ccgctctatc gggttcggaa
     5221 ccgaaccacg accgagtacg cgtacaatga gaagcagcgc gacgatgcgg tggcccggct
     5281 tcggggcgcc gaagtgaccc gcttcaaggg ccttggcgag atcgatcccc gggagttcgg
     5341 acaattcatc ggcgaggata tgcgcctcat ccgggtaagc cttcccgggg tgacggagat
     5401 gcaccggagc cttgagttct acatgggcag caacaccccc gaacgccgct ccttcatcat
     5461 ggagaatctg atctgatgac tcaggcgcat caacttttcc gccaaaactt tttagagtac
     5521 gcctcctacg tgatcaagga gcgggcgatc cccgatctgg tggacggctt caaaccggtt
     5581 caacgacgaa tcatccatac cctcttcgag atggatgacg gcaaattcca caaggtcgcc
     5641 aacacggtgg gttgggcgat gcgcttccat ccccacggcg atgcctcgat ctatgaagcg
     5701 ctggtgaacc tcgccaacaa cgatctgttc atcgaccggc agggaaattt cggaaacatc
     5761 ctcaccggcg acagcgccgc cgccccccgg tatatcgtat gccgtcttct gcttgtagca
     5821 aaagaggttc tctacaaccc cgagttgacg gagtgggtcg aatcctatga cgggcgaaac
     5881 aaggagccgg tggtgttccc cgccaagatt cccgtcgtcc tcattcaagg agtcatgggg
     5941 atcgcagttg gaatgtcaac cctgattctc ccccacaacc gctttgaagt ccttgatgcg
     6001 atggccgccc acttacgggg agaatccttt gaactctatc ccgacttccc tcaaggggga
     6061 atcgtcgacg tcgaagacta caacgacggg aagggaacgg ttctggttcg ggcgcggctc
     6121 aatacaagcg acccgaagcg gatcatcgtt gaagagcttc cctatggaac gaccagcgaa
     6181 tccctgatca attcgattga ggacgcggcg aagaaaggcc gactgaagat cagctcaatc
     6241 aacgatttca ccgccgagag cgtgaacatc gagatcaacc tcgcccggaa cacctattcc
     6301 aaggacatcg tcgacgccct ctacgcgtat acggattgtg aaaagcggat caccaccaac
     6361 ccgctggtga tcagggacaa cgtccccacg gtcatggggg tcagcgaaat cctcgccttc
     6421 cacgccgagc acctcaaagt ggtgctgaag gcggagctgg agctggagga ggagaagctg
     6481 cttgcaaaga tgcacgaccg cacgctggag cggatcttca tcgaagagcg gatttacaag
     6541 cggattgaaa cgaaaagaag cgcggacgat gtcctcaagg cggttcatac cggcttgaaa
     6601 ccgttttcca gtgaactgct ccgtgatgtt accgacgagg atgtcgaacg gcttttgaaa
     6661 atcccgatcc ggaggatcag cctcttcgac attgagcgga accgtcagga gatcgagcag
     6721 atcaatgaac agttggagga ggtccgatcg aagctcgaga acctcaccgg ctatgccttg
     6781 gagacgattg atcgcctgaa ggggatgttc gacaggaaag agcacaagag acgatcgacg
     6841 ctgaagaagt tcgagctggt ggatgtgaag gaggtcgccg tccgcgacct cgttgtccga
     6901 tacgattcga ccaacgggta tctgggctac ggggtgaaga cgggagccga gcaggtgacg
     6961 gtcagccaat tcgaccgcct cctggtgatc cgccgcgacg gttcctatca ggtgatcgac
     7021 gccccggaga a
//
LOCUS       DAOPUZ010000009         6677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_11423_length_6677_cov_3.145877, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000009 DAOPUZ010000000
VERSION     DAOPUZ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11423_length_6677_cov_3.145877"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PKN79_00390"
     CDS             complement(<1..983)
                     /locus_tag="PKN79_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94261.1"
                     /translation="MSEKKRYGRQIFISFIVTIVILLGGVYLATQYLLPHVKWDQDQL
                     FSFFVKLFPLLIGLIMIEIGVVISKKRDEDFADDIDKLPPNAYDRPFYALPQDDPAFI
                     RNDEVIYAQAPVLEEATFTEAVLSEEPIVERAAEVILPLDLPQVEEEPEVLVVEPVAE
                     TVATELEEPILETVAAEEKEPVVGEAAPVVPPYQAPRDEISIAIEEGILQVYEMDFDS
                     ILELELESANDLNYDLTVVIVRVTEGPVDAIAQKLIRQSGDLSYTFTLEDNTIGIILP
                     FYNGDEARTFTLSLIESCQQEFQGSELEIGFASLELLLAGFDQGKGEGAGN"
     gene            1061..1495
                     /locus_tag="PKN79_00395"
     CDS             1061..1495
                     /locus_tag="PKN79_00395"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNZ94262.1"
                     /translation="MLKRSLLAGLLTYFFTFLLLLAVFGQGGYLHNRALEAEIQRLSY
                     NQNLLQIQIQALERQEVQMGKEDALRDAAFKYGYQSEGEVVYYFPLEERESPDILEDR
                     PIEGRREYFEGVGRSVLALWALLPAFLAGMLAYVIGRRRFRG"
     gene            complement(1485..2804)
                     /locus_tag="PKN79_00400"
     CDS             complement(1485..2804)
                     /locus_tag="PKN79_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNZ94263.1"
                     /translation="MPTTLKRLATYGVQPPTILIPKHEIDLKKWAVVACDQYTSEPEY
                     WKRVEAYVGDAPSTLKLIYPEVYLEEKNSQERINAIHQAMNRYLEADLFDIYEESFFL
                     IHREDEGRSSGRWGLLAALDLEHYDWKSGSHTLIRASEETILDRIPPRKLIRTGAVLE
                     LPHILVLIDDPDETIIEPLSTNRSSFHQVYSTSLMEGGGTVTAWRINDEQEFSRLADR
                     FQALLERQYTNNPLLFAMGDGNHSLATAKSCWLDIRKGLSEEEISNHPARWALVEIQN
                     IYDPGIVFEPIHRLLFSIEHQSFLDALGSFCASYETKSLFSIDELDRYLALDDGVQRF
                     GYKDEERFLLFSLKDPASSLAAGTIQHVIDRLLDQQSATVDYTHGEDVTLRLGSLPGN
                     SAVILPNFPKSAFFKTVKKEGRLPRKTFSMGHAHQKRFYLEARKITL"
     gene            2871..3497
                     /locus_tag="PKN79_00405"
     CDS             2871..3497
                     /locus_tag="PKN79_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphokinase"
                     /protein_id="HNZ94264.1"
                     /translation="MSHVLIITGGGAPKALEALHLEAYRFIVVADSGIEFAVRHDLFA
                     DIWVGDFDSAKIKPDRVGEIIHKDHDMKLESDTELALMIGVERGMTTYTLIGGGGYRM
                     DHLLQTYSLFGRYGLPLAWHTKYESLYSVHRERRFITSAGRTLSFLPASFTGRSIVNA
                     SELVWPLESYQLTTKSISLSNRTTADLLNVTVEGDPIFLCFPVADIGP"
     gene            3514..3864
                     /locus_tag="PKN79_00410"
     CDS             3514..3864
                     /locus_tag="PKN79_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94265.1"
                     /translation="MLHERRLLAFSSRVHTYTLFLYLFFFLLYVLGGFFLVDTSFETL
                     LSFLLKLVGYSSIIFGVWVLAFSLVVWFFDKVFPFSPIFFTILRIGAVILMATIIEII
                     NTLVHRGLSIQWGG"
     gene            3866..4240
                     /gene="aroH"
                     /locus_tag="PKN79_00415"
     CDS             3866..4240
                     /gene="aroH"
                     /locus_tag="PKN79_00415"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNZ94266.1"
                     /translation="MDTPRISAVRGAIQVASDSKEAIAQAAQKLFVRVLETNDLHEEQ
                     IASLLITQTGDLKSLNPATGLRMGGLASYVPLFCMPELEIEGMMKRVIRMLFILKIHK
                     NDLQPIYLDGAELLRPDLMRNE"
     gene            4237..5565
                     /gene="aroA"
                     /locus_tag="PKN79_00420"
     CDS             4237..5565
                     /gene="aroA"
                     /locus_tag="PKN79_00420"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNZ94267.1"
                     /translation="MTVTIQRGALNGTMAVPGSKSDTIRAVLLALLSEGRSVIRNPLA
                     SGDGLSAKRAAIALGASITEEGNCWIVEGVGRNLRAPQTSLDLGNSGTATSFLSSIVT
                     LAGKPVTLTGDEQIRRRPFKTLFDALGDLGAGVSVHNPNQVSPPVTISGPLHGGPVTL
                     QGFNSQFVSSLLLASPFAETPVTVTVRNGLEKPYVRLTLDWMKRFGIEVANPSDYEYF
                     EIPNTKTYRAGEVAVGGDWSAVAFPLVASALTPSELTITGLNFHDSQGDKRVVDILKA
                     MGADIVEDHREGTLSIRGGKPLSALQEIDLADIPDALPALVVAATQAHGTTTFTNISH
                     VRVKETDRVFEMSERLNRLGCKLEAGENELSVHGPTPIGGGTVSSAGDHRIAMAMVAA
                     GLAAREAITITETECVAVSFPNFFERFEECGARIKKRHTEVHRREADCQN"
     gene            complement(5547..5618)
                     /locus_tag="PKN79_00425"
     tRNA            complement(5547..5618)
                     /locus_tag="PKN79_00425"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5584..5586),aa:Thr,seq:tgt)
     gene            5740..5822
                     /locus_tag="PKN79_00430"
     tRNA            5740..5822
                     /locus_tag="PKN79_00430"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5774..5776,aa:Tyr,seq:gta)
     gene            5882..6322
                     /locus_tag="PKN79_00435"
     CDS             5882..6322
                     /locus_tag="PKN79_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNZ94268.1"
                     /translation="MSRFYDEGLQFSCVAGCVYCCGVEPGYVFLSQADLDRLCAHTRM
                     DQEAFIKTYCHYVDMGAYKMISLVEKKNHDCIFLSPNGCTVYEARPVQCRTYPFWGTI
                     IESRETWEEEARSCPGIGHGKRYSKKEIDGFIRQRIANGLIMII"
     gene            6331..>6677
                     /locus_tag="PKN79_00440"
     CDS             6331..>6677
                     /locus_tag="PKN79_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HNZ94269.1"
                     /translation="MAKIAESTLQQIKARLSISEVVSDYVTLSSRGGRLWGLCPFHEE
                     RTPSFSVVDQEGFYYCFSCKKGGSIFDFIMETEKVPFHEAVKMLAQRAGVELEEETPA
                     QRAQRDLSENLYEL"
BASE COUNT         1522 a         1724 c         1848 g         1583 t
ORIGIN      
        1 ctgttgcccg caccttcacc ctttccctga tcgaatcctg ccaacaggag ttccagggat
       61 gcgaacccga tttccaattc gcttccctgg aactcctgtt ggcaggattc gatcagggaa
      121 agggtgaagg tgcgggcttc atctccgttg tagaagggga ggatgatgcc gatggtattg
      181 tcctcgaggg tgaacgtata ggaaaggtcc ccgctttggc gaatcagctt ctgagcgatc
      241 gcgtcaaccg gtccttcagt aactcgaacg atcacgacgg tgaggtcata gttcagatcg
      301 ttcgcgctct caagctcaag ctccaggatc gaatcgaaat ccatctcgta cacttgaagg
      361 attccttcct cgatggcgat ggaaatctcg tcacgcggcg cttgataggg gggaacaacc
      421 ggtgccgctt ctccgacgac cggctctttt tcctctgctg cgacggtctc gagaatcggt
      481 tcttcaagct cagttgcgac ggtctcggcg accggctcga cgaccagaac ctccggctct
      541 tcttcgacct ggggaagatc gaggggaagg atcacttcgg cggcccgttc aacgatcggc
      601 tcttcactga gaaccgcttc ggtgaacgtt gcttcttcaa ggacgggagc ttgggcgtag
      661 attacttcat cgtttcggat aaacgcggga tcatcctggg gcagggcgta gaaaggtcga
      721 tcgtaggcgt tggggggaag cttgtcgata tcgtcggcga aatcctcatc ccgcttctta
      781 gatatgacaa cacctatctc aatcatgatc aagccgatga ggaggggaaa cagcttcacg
      841 aagaaagaaa acagttgatc ttgatcccac tttacgtgcg gcaggagata ctgggttgcc
      901 agatacactc ccccgaggag gatgacaatc gtgacaatga aacttataaa aatttggcgc
      961 ccataccgtt tcttttcaga cattcctacc tccacaacaa ggtcagattc ctatataata
     1021 cccatagtat catactttac cacaaatatt aaagatttat atgcttaaga gaagcctgct
     1081 ggcgggttta ttgacgtatt ttttcacatt tctccttctt ctggctgttt tcggtcaagg
     1141 aggatacctg cacaaccgag cactcgaagc ggaaattcag cgtctttcgt ataatcaaaa
     1201 ccttcttcag attcaaatac aggcccttga acgtcaggaa gttcaaatgg gcaaggagga
     1261 tgcccttcgg gatgccgcct tcaagtacgg ctaccaaagt gaaggagagg tcgtatacta
     1321 cttccccttg gaagagcgcg aatcccccga cattcttgaa gaccggccga ttgaaggcag
     1381 gcgggagtac tttgaaggag tcgggcggag tgtgctggcc ctctgggcgt tgctccccgc
     1441 atttcttgca ggcatgcttg cctatgtcat aggcaggcgc cgctttagag ggtgattttt
     1501 cgagcttcga ggtagaagcg cttttgatgg gcgtgtccca tcgagaacgt cttcctcggt
     1561 aatcggcctt ctttcttgac ggttttgaaa aacgccgatt tgggaaaatt gggaagaatg
     1621 accgctgaat tgcccggaag cgatcccagc cgaagcgtga catcttcgcc gtgcgtgtaa
     1681 tcaacggtcg ctgattgctg atccaacaat cggtcgatca cgtgttggat ggttccggcg
     1741 gctaacgaag aggccggatc cttcaacgaa aagaggagaa agcgctcctc gtccttatat
     1801 ccgaaccgct gcacaccgtc atccaaggcg aggtagcggt ccagctcatc gatggagaag
     1861 aggctttttg tttcgtagga ggcgcagaag cttccaaggg catcaagaaa tgattgatgc
     1921 tcgattgaaa agagcagccg gtgaatcggt tcgaagacga tgcccggatc atagatgttt
     1981 tggatttcaa ccaacgccca gcgggccgga tgattggaaa tctcctcttc ggacaaccct
     2041 tttctgatgt caagccagca gctctttgcc gtcgccagac tatggttgcc gtcacccatc
     2101 gcaaagagga gcgggttgtt cgtgtattgc cgctcgagca atgcctggaa ccgatccgcc
     2161 aaacgactga actcctgttc atcgttgatc cgccacgccg ttaccgttcc gcccccctcc
     2221 atcaaagaag tggagtacac ctgatgaaag gaagaccgat tggtgctcaa aggttcgatg
     2281 atcgtctcat ccggatcgtc gatcagaacg aggatatggg gcagctccaa gaccgcgccg
     2341 gtccggatca gcttgcgggg aggaatccga tcgagaatcg tttcctcgct tgcccggatg
     2401 agcgtatgcg agccgctctt ccagtcgtaa tgttccagat ccaacgccgc aagaagtccc
     2461 catcgaccgg aagagcggcc ttcatcctcc cgatggatga ggaagaagct ctcttcgtag
     2521 atatcaaaga gatcggcctc aagatatcga ttcatcgctt ggtggatcgc attgattcgt
     2581 tcttgggaat tcttctcttc aaggtatact tcggggtaga tcagcttcag tgtggaagga
     2641 gcgtcaccca catatgcttc gactcgtttc caatactccg gctccgaggt gtactggtca
     2701 caggcgacga ccgcccactt ctttaaatcg atctcgtgtt tcggaatcag gatcgtcggc
     2761 ggttgaacgc catatgttgc taatcgctta agcgtcgtag gcattggtat cctctttatt
     2821 gtgtgttatt gtgcgtaagt gtatcgaaag gcgaaaggga gggaaagagg atgagccatg
     2881 tgttgattat caccggaggg ggagcgccga aggctcttga agctcttcat ctcgaagcct
     2941 accggttcat cgtggtcgcc gacagcggca tcgagttcgc ggtaagacac gacctgtttg
     3001 ccgatatctg ggtcggagat tttgattccg ctaaaataaa gccggatcgc gtcggcgaaa
     3061 tcattcacaa agaccatgat atgaagcttg aaagcgatac ggagctggcc ttgatgatcg
     3121 gagtggagcg ggggatgaca acgtatactc tcatcggtgg cggcgggtat cggatggacc
     3181 atcttcttca gacgtattcg ctcttcggtc gctacggtct tccgcttgcc tggcacacca
     3241 agtatgagtc actgtacagc gttcaccggg aacggagatt catcacatcg gcgggccgga
     3301 cgctcagttt tctgccggca tccttcaccg gaaggagcat tgtcaacgct tccgagctgg
     3361 tctggccgct ggaaagctat cagttgacga cgaaatcgat ctcgctttcc aaccgcacga
     3421 ccgccgatct gctgaatgta acggttgaag gcgatccgat cttcctctgc tttccggttg
     3481 cagatatcgg gccttaggtg ctacgatcga agcatgctgc acgaacgaag actgctggcg
     3541 ttttccagtc gggtccacac ctacactctc tttttgtacc tgttcttctt ccttctctat
     3601 gtgctgggcg ggtttttcct cgtcgacaca tcctttgaaa cgttgctgag ctttttgctg
     3661 aagctggtcg gatactcaag catcatcttc ggagtatggg tactggcgtt ctccctcgtc
     3721 gtctggtttt tcgataaggt atttccgttt tcaccgatct tttttacgat tctgagaatc
     3781 ggtgcggtga ttctcatggc gacaatcatc gaaatcatca atacgttggt ccatcgagga
     3841 ctttcaatcc agtggggcgg gtaagatgga tacacctcga atctcggcgg tgcgcggcgc
     3901 cattcaagtc gcttccgatt ccaaagaagc aatcgctcaa gcggcacaaa aactctttgt
     3961 acgcgtattg gaaacaaacg acctgcacga agagcagatc gcatcgttgc tgatcaccca
     4021 gaccggggat ctgaaatccc tgaatccggc gacgggcttg aggatggggg ggcttgcttc
     4081 ctacgtgccg ctcttctgca tgccggagct tgaaatcgaa gggatgatga aacgggtgat
     4141 ccggatgctg ttcatcctga agatccacaa aaacgatctt cagccgatct accttgacgg
     4201 ggcggaactc ctgcggcccg atctgatgag gaacgaatga ccgtaacgat tcaaagggga
     4261 gccctgaatg gaacgatggc cgttcccggc tctaaaagcg acacgatcag ggctgttctt
     4321 cttgcgctgc tttccgaagg acgttcggtc atccgcaatc cgctggcctc cggagacgga
     4381 ctttccgcaa aaagggcggc gatcgctctt ggagcttcca ttacggaaga aggaaattgt
     4441 tggatcgttg aaggagtggg gcgtaatctc cgagcacctc aaacgagcct tgatctggga
     4501 aacagcggaa cggcgacctc cttcttgtct tcaatcgtca cgctggccgg aaagccggtt
     4561 acgctcaccg gcgatgaaca gatccggcgc cggccattca aaacgctgtt cgatgccctc
     4621 ggggatctgg gggcaggggt ttcggtgcat aatcccaatc aggtctcccc gccggtcacg
     4681 atctcaggcc ctcttcatgg aggacccgtc acgcttcaag gcttcaacag ccaattcgtc
     4741 tccagtctac tactggcgag cccgtttgcg gaaactcccg tcaccgtcac cgtccgcaac
     4801 ggattggaaa agccgtatgt ccggttgacg ctcgattgga tgaagcggtt cgggatcgag
     4861 gtggcaaacc cgagcgacta tgaatacttt gagattccga atacgaagac ctaccgggca
     4921 ggtgaagtag ccgtgggagg cgattggtcg gcggtggcgt ttcctctggt cgcttccgca
     4981 ttgactccct ccgaactcac gatcaccggc ctgaacttcc atgacagcca gggggataaa
     5041 cgggtggtcg atattcttaa agcgatgggt gccgacatcg ttgaagatca tcgggaagga
     5101 accctttcaa ttcgcggcgg gaaaccgctt tccgccttgc aggaaattga tcttgcggat
     5161 atccccgatg cgcttcccgc ccttgtcgtg gcggcgactc aggcgcatgg gacgacgacg
     5221 ttcaccaaca tctcccatgt ccgggtcaag gaaaccgacc gggtcttcga gatgtccgag
     5281 cggctcaacc gattgggctg caagcttgag gcgggggaga atgagctctc cgtccacggt
     5341 ccgactccca tcgggggtgg aacggtttcc tccgccgggg accatcggat cgcaatggcg
     5401 atggtcgcag ccggtctggc tgcaagggag gcgatcacga taacggaaac ggagtgcgtc
     5461 gcagtctcct ttcccaactt ctttgaacgc tttgaagagt gcggggcaag aataaaaaaa
     5521 cggcataccg aagtacaccg ccgggaagcc gactgtcaga attgaactga cgaccccgag
     5581 attacaagtc acgtgctcta ccgctgagct aagtcggcat cgcgtgcaac tatatctggc
     5641 ggagcatgga cgtgtcaaga agcgggtttt tgggcaaaat cccttcgtgc ggttgacaaa
     5701 gggcccaata gcttgtattg tacgtttgtc ttttgacagg gagaggtacc caagcggcca
     5761 aagggggcag actgtaaatc tgttggttat caccttcgaa ggttcgaatc cttctctctc
     5821 caatcggagg ccttcttcta cgaggaaggc ctttgttcgt catcgtgaaa aaggggtata
     5881 gatgagtagg ttttacgatg aaggcctgca attctcctgc gtagcaggct gcgtctattg
     5941 ttgcggggtt gagccggggt atgtctttct ctctcaagcc gatctcgatc gcctttgtgc
     6001 ccatacccgc atggatcagg aagcattcat caagacgtac tgtcactacg ttgacatggg
     6061 ggcttacaag atgatcagcc tggtggagaa aaagaaccat gactgcatct tcctgagccc
     6121 gaacggatgc accgtgtatg aagctcgtcc ggttcaatgc cgaacctacc cgttttgggg
     6181 cacaatcatt gaaagcagag agacatggga agaagaagcc cgttcatgtc ccggaatcgg
     6241 acacggtaaa cggtacagca agaaggaaat tgatggtttc attcgtcagc gaatcgccaa
     6301 tggattgatc atgatcatat aggagaccag atggcaaaga ttgctgaatc tactctccaa
     6361 caaatcaaag ctcgcctgtc gatcagcgaa gtggtcagtg attacgtcac cctctcctcg
     6421 agaggcgggc gcctctgggg attgtgtcct ttccatgaag agcgcacccc ttccttcagc
     6481 gtcgtcgatc aagaggggtt ttattactgt ttttcctgta aaaaaggggg atcaatcttc
     6541 gacttcatca tggagacgga aaaggtgccg ttccatgaag cggtgaagat gcttgcccaa
     6601 cgagcgggag tggaactgga agaggagacc cccgcccagc gggctcagcg ggatctttcc
     6661 gaaaacctgt atgagct
//
LOCUS       DAOPUZ010000010         6610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_11560_length_6610_cov_3.670175, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000010 DAOPUZ010000000
VERSION     DAOPUZ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11560_length_6610_cov_3.670175"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..657
                     /locus_tag="PKN79_00445"
     CDS             112..657
                     /locus_tag="PKN79_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNZ94270.1"
                     /translation="MIYLTLFYEFFKIGLFSIGGGLATLPFLYKLAEKRPDWLTIQTI
                     ADMIAVSESTPGPIGINMATYAGYQVAGIFGGIIATLGEVAPSIIIIVIIAKFLAEFD
                     KNPLVKHAFYGLRAAVVGLITFALLKLLSVTLLTDGAINYLNAGLYLVFVALVLIFKK
                     AHPLIWILLGALIGLMLGLPS"
     gene            complement(626..1198)
                     /locus_tag="PKN79_00450"
     CDS             complement(626..1198)
                     /locus_tag="PKN79_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ94271.1"
                     /translation="MKYVGITTILLALLISGCSSTNAERLTLDAFQHIDEGRIEEALP
                     LFEDACRKQPENPEYLYNLLYAKLQNKEFESVIEKSGEAFMRHPLYLEFLYLKARAQR
                     ELNRTDAAIATYREIARLNPGDVKALALLLSEAATHGLQEEAVYLAQVMLDADPSNMT
                     ALKTLAEADPSSWFALVYAYLMKEDPASVQ"
     gene            complement(1195..2259)
                     /locus_tag="PKN79_00455"
     CDS             complement(1195..2259)
                     /locus_tag="PKN79_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNZ94272.1"
                     /translation="MNKITLHATDLDGYLLDADKAKIDEANRLYKEYLEHCSKLDRAM
                     NHDETVAERNNLVVKAREIGRFLKEVCSNEPNIHVYSFETPQEQHGAASRLISKLRNP
                     VTGNEEFLYYVQRAYELLFSHVWADAVLPTKRSVITRTPVTVPVQNYAVHRIPDVDSI
                     INNSVMCVMLRGALLPSMIVSKEIQEYSSIGYVTPFALFKIKRDDSKKESNMDYILDL
                     DKSYFDLQQLDGKDLIFADPMNATGGSLVTIAKYLKEHNVRPKSVKFINIISALKGSL
                     RVVRAIEGAQVYTLWMDPVLNDAAYILPGLGDAGDRLNGKDSESNPRNIIQLLADYGA
                     GITNLYRSQVREIEQTVLER"
     gene            complement(2315..3151)
                     /locus_tag="PKN79_00460"
     CDS             complement(2315..3151)
                     /locus_tag="PKN79_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ94273.1"
                     /translation="MSTSIKRQKRNLGSHIILIAWSFIVLFPIWTLVINSFKTRLTIY
                     ENPFWIPKVWNFTNYATVLRDGDFITYFKNTFIVVTLSLALVSFFASLAAYAFARNKG
                     KIYTLLYYFFIAGLMLPIKIASIRILDIVRSLGLLNTIWALPPIYIAMGLPVGIFILT
                     TFIRDLPSALSEAAHIDGASSWQIYALVILPLIRSAMATVTIYNLIPFWNDLWFPLIT
                     INDESQKTLLLGVTRLFGQYQTDWSKVLAVLTLAAIPVLGLYLGMSSQFIKGVTAGSV
                     KG"
     gene            complement(3153..4052)
                     /locus_tag="PKN79_00465"
     CDS             complement(3153..4052)
                     /locus_tag="PKN79_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ94274.1"
                     /translation="MKKNNLQWGLYIIPALLVYTVFMAFPLLDSLRLSLFTGNTPQTR
                     SFVGFGNFVRLFTDKTISVRYWGAFKNTWVFFAIHMVVQNGLGILFAVLLTQKDMKGR
                     HTYQTIIFIPTTFAVLITGYLWKLILNPVWSGDFFTSIGLGFLSYPYLGDTKTALVTV
                     SLVSCWQWLGIPTMMFVAALRNISEDLLEACALEGASKWQTFRHVKLPLIKPVVGIIA
                     VLTFVNNFNAFDIVFAMENVNGAPEYSTDLIGTLFYRYGIAGQHPVGIPDIGLGAAIA
                     TITFFLLLVVVVPTLTKTQQGGY"
     gene            complement(4105..5331)
                     /locus_tag="PKN79_00470"
     CDS             complement(4105..5331)
                     /locus_tag="PKN79_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ94275.1"
                     /translation="MNKKTLFFAVLLLLLVLLPLTAQGAKAPSAVTLTMGSWRADDVE
                     QMNRVLAEYKKVAPDVTIRFQPTNPPDYNATLRLQLDSGTGPDLMYARSYAAGEDLFN
                     AGYFADVTDIPGVLQNFTASSLAPWQTANGRMFAVPFAAVSHAVYYNKDIFKAEGLSI
                     PQTWEEFLSLAKTLKNRGYTALANGLADEWDILETFFFGLLPNFIGGAEQRVKYERGE
                     LKINDANFVAAYQAMADVAPYCPKGFESVTYNDSQVLFNSQKAVMFVDGSWTSEVYKD
                     ASFNWGVFAIPAPKGKKTAICFHPDMAITMNTKTAHPEEARAFLEWLATKEGATIASE
                     NLPVGFFPMINFKITLANQQANEFLSLNAGKETDARFVWPALMDLYAPMNQAVIRVMK
                     GQITAKQAADSVAALL"
     gene            5476..6117
                     /locus_tag="PKN79_00475"
     CDS             5476..6117
                     /locus_tag="PKN79_00475"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HNZ94276.1"
                     /translation="MNLIFLGPPGAGKGTVADLAKDVLGIPHISSGQLFREQIKIGSP
                     LGKRVEATLKAGNLVDDETTIEFVRERLSRDDVARGYILDGFPRTIAQAEAMCVFSKP
                     EAVINFSLTEEEILRRLEGRRVAPTTGRTYHILYNPPKVEGLDDETGETLIQRPDDKK
                     EAILNRLEVYTRETRPLINHYREKNLLVPLDASPSPQEVLNQLLHLLKEKGIH"
     gene            6128..>6610
                     /locus_tag="PKN79_00480"
     CDS             6128..>6610
                     /locus_tag="PKN79_00480"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNZ94277.1"
                     /translation="MATYHNEPSRTFSEYLLIPGYSDRNCVPANVSLATPLVRYRKGM
                     EECPLSLQIPLTSAIMQSVSGEKMAIALAREGGLSFIYSSQPIQDQEAMVRRVKGYKA
                     GFVTSDSNLTPNHTLGDLIALKEEKGHSTIAITDDGTSSGTLLGLVSSRDWRPSRTSP
                     D"
BASE COUNT         1561 a         1682 c         1833 g         1534 t
ORIGIN      
        1 tcttgttgtg gcttcgacgt tgatgttttc gctttctccg atcattgtcg tcatcgtcgc
       61 cattcttttc ggtatcgtag ttcagaacat aaagtgcaag aaggaacgga catgatctat
      121 ctcacgctct tctacgagtt cttcaagatc ggtctgtttt caatcggagg agggttggcc
      181 accctaccgt tcctctacaa gcttgcagaa aaaagacccg attggcttac catccaaacg
      241 atcgccgaca tgatagcggt cagcgaatcg acccccgggc cgatcggaat caatatggcg
      301 acctacgccg gctatcaagt ggcgggaatc ttcggcggaa tcatcgccac gctcggcgaa
      361 gtcgccccct cgatcatcat catcgtgatc atcgccaagt ttttagcgga gtttgataag
      421 aacccgcttg taaagcatgc attctacggc ctgagggccg ccgtagtcgg actgatcacc
      481 ttcgctcttt tgaagctgtt gtccgtcacc ctgctgacag acggtgcgat caactacctc
      541 aatgccggct tgtacctcgt ctttgttgcg ctcgtgctca tcttcaagaa agcccatccg
      601 ctgatctgga tactcttggg agctcttatt ggactgatgc tgggtcttcc ttcataaggt
      661 acgcatagac aagcgcaaac caagaggaag gatccgcctc cgccaatgtt ttcagtgcgg
      721 tcatgtttga gggatccgca tccaacatca cttgtgcaag gtacacagcc tcttcctgca
      781 agccgtgcgt tgccgcctcc gacagaagaa gggcaagggc tttcacatct cccggattca
      841 acctggcgat ctcccgatac gtcgcgatcg ccgcatccgt tcggttcagt tcccgctgcg
      901 cccgggcttt caggtaaaga aattcaaggt ataggggatg gcgcatgaac gcctctccac
      961 ttttttcgat caccgattca aactccttgt tttggagctt tgcataaaga agattgtaca
     1021 gatactcggg gttttcgggt tgcttccggc aggcatcttc aaagaggggc aaggcttcct
     1081 cgattctgcc ttcatcgatg tgctggaacg cgtcaagggt gaggcgctcg gcattcgtgg
     1141 aggaacatcc gcttataagg agagcaagaa ggatggtcgt gatgccgacg tacttcatcg
     1201 ctccaagact gtctgttcaa tctcccgtac ttgcgaacgg tagagattgg tgattccggc
     1261 accgtagtcg gccaatagct ggatgatgtt gcggggattg gattcgctgt cctttccgtt
     1321 gagccggtca ccggcatcgc caagtcccgg gaggatatat gcagcatcgt tgagcacggg
     1381 atccatccaa agggtataca cttgagctcc ttcgatcgcc cggacgaccc tcagcgatcc
     1441 tttgagggcg ctgatgatat tgatgaattt cacgcttttg ggtctgacgt tgtgttcttt
     1501 caggtatttg gcgatcgtaa cgagcgaacc gccggttgcg ttcatcggat cggcaaagat
     1561 caaatccttg ccgtccaact gctgaagatc gaagtaggat ttatccagat cgaggatata
     1621 atccatgttc gactcctttt tggaatcatc ccgcttgatc ttgaacaagg cgaagggtgt
     1681 gacgtatccg atggaagagt actcctgaat ctccttgctc acgatcatcg aaggaagcag
     1741 cgccccgcgg agcatcacgc acattaccga gttgttgatg atcgaatcaa cgtcggggat
     1801 tcggtgcacc gcatagttct ggaccgggac ggtgacgggg gtgcgggtga tgaccgagcg
     1861 cttggtggga agaaccgcgt ccgcccaaac atgtgaaaag aggagctcat acgcccgttg
     1921 aacatagtag aggaactcct cgttgccggt gaccgggttt ctcagcttgc tgatcaggcg
     1981 gctggccgca ccgtgctgct cctgcggcgt ttcaaaggaa tagacgtgga tattcggttc
     2041 gttggaacac acttctttca aaaagcgccc gatctcccga gcttttacca caagattgtt
     2101 tcgctcggcg acggtctcgt cgtggttcat ggcgcgatca agtttcgaac aatgctccaa
     2161 atactctttg tacagcctgt tcgcttcatc gatctttgct ttatcggcat caagcaaata
     2221 cccgtcaagg tccgttgcat ggagggtgat cttgttcatc ggctcgttct cctacgccga
     2281 ttctagcgta agatcgcccc ttgcacaacc gtgatcatcc cttcaccgaa ccggcggtaa
     2341 cccctttgat gaactgagag gacatcccaa ggtacagacc gagcacggga atcgccgcaa
     2401 gggtgagcac ggcgagaact ttcgaccaat cggtctggta ttgaccaaag aggcgggtaa
     2461 ctcccaacaa aagggtcttt tggctttcat cgttgatggt gatcaagggg aaccagagat
     2521 cgttccagaa aggaatcaga ttgtagatcg tcaccgttgc catggcgctg cgaatcaatg
     2581 ggagaatgac cagtgcatag atctgccagg atgacgcccc gtcgatgtgg gcggcttcgc
     2641 tgagggcgga gggaaggtcg cgaatgaagg tggtgagaat gaagattccc accggaagcc
     2701 ccatagcgat atagatcggc ggcagcgccc aaatcgtgtt gagcaatccg agcgagcgga
     2761 cgatgtcgag aatccggatc gaagcgattt tgatcggcag catcaagccg gcgatgaaga
     2821 agtaatagag cagggtatag atctttcctt tgtttcgggc aaacgcatag gcggccaatg
     2881 aggcgaaaaa ggagacgagc gcgagggaga gcgtcacgac gatgaaggtg ttcttgaaat
     2941 aggtgatgaa atccccatcc cgaagaaccg tcgcatagtt ggtgaaattc cacaccttgg
     3001 gaatccagaa cgggttttca tagatcgtca aacgggtctt gaacgagttg atgaccaagg
     3061 tccagatggg aaagagcacg atgaaggacc aagcgatgag gatgatatgg ctgccgaggt
     3121 ttcttttttg gcgttttatg gaggtcgaca tctcaatatc ctccttgctg ggtcttggtg
     3181 agggtgggaa cgacgacaac gagcaacagg aagaaggtga tggttgcgat tgcggcgcca
     3241 aggccgatat cggggattcc gaccgggtgc tggccggcga tgccgtaccg gtagaagagg
     3301 gttccgatca ggtcggtgga atactcgggc gctccgttga cgttctccat ggcaaaaacg
     3361 atgtcaaacg cgttgaagtt gttgacgaag gtgagcacgg cgataatccc caccaccggc
     3421 ttgatcaaag gaagtttgac atgccggaag gtttgccatt tgctcgctcc ctccaatgca
     3481 cacgcttcca agaggtcctc gctgatgttt ctcaaggctg cgacgaacat catcgtcggt
     3541 attccaagcc attgccagca ggaaaccaga ctgaccgtga ccagagcggt ctttgtatct
     3601 ccgaggtagg gatagcttaa aaaaccgagg ccgatcgagg tgaaaaagtc tccgctccac
     3661 acgggattga gaatcaattt ccaaaggtat ccggtgatca gaacggcaaa cgtcgttgga
     3721 atgaagatga tcgtctggta ggtgtgccga cctttcatgt ccttttgcgt cagtaaaacc
     3781 gcaaaaagaa tccccagtcc gttttgaaca accatgtgaa tagcgaaaaa gacccaggta
     3841 tttttaaacg ctccccaata gcggacgctg atcgtcttgt cggtgaagag tcgaacgaag
     3901 tttccgaagc cgacgaaact ccgagtttga ggagtattgc cggtaaagag actcaagcgc
     3961 agggagtcaa gaagaggaaa cgccatgaat accgtgtata cgagcaatgc ggggatgatg
     4021 tacaagcccc attgtaggtt gttctttttc atattactcc aacaaaatgc ccctcctccg
     4081 gatgagagaa ggggccggtt acacctaaag cagtgcagct accgaatcgg cggcttgttt
     4141 tgccgtgatc tgacctttca tcacgcggat gaccgcttgg ttcatcggag catagagatc
     4201 catcaaagcc ggccagacga atcgggcatc ggtttccttg cccgcgttga gggagaggaa
     4261 ctcgttggcc tgttggttcg ccaaggtgat cttgaagttg atcatcggga agaatccgac
     4321 ggggaggttt tcactggcga tcgtcgcacc ttccttcgtt gcaagccatt cgaggaatgc
     4381 ccgcgcttcc tccggatggg cggtcttggt gttcatcgtg atcgccatgt ccgggtggaa
     4441 gcagattgcg gtcttcttgc ccttcggtgc cgggatcgcg aagacgcccc aattaaagga
     4501 tgcatccttg tatacttcgc tcgtccagga gccgtcgacg aacatcaccg ctttctggct
     4561 gttgaagagc acctggctgt cgttgtacgt taccgactcg aagcccttcg gacaataggg
     4621 ggcgacatcc gccatcgcct gataggcggc gacgaagttc gcgtcgttga tcttcagctc
     4681 acctcgttca tatttgaccc gttgctccgc cccgccgatg aagttcggca gcagaccgaa
     4741 gaagaaggtt tccagaatat cccattcatc tgcaagcccg ttcgccagtg cggtatatcc
     4801 gcggttcttg agggttttag cgagggagag gaattcttcc cacgtctggg gaatcgaaag
     4861 accttccgct ttgaagatat ccttgttgta gtagacggcg tggcttaccg cggcgaacgg
     4921 aacggcgaac atccgcccgt ttgcggtctg ccagggagcg aggcttgaag ccgtgaagtt
     4981 ttgaaggacg ccgggaatat ccgttacatc ggcaaaatat ccagcattga aaagatcctc
     5041 gcccgctgcg taggatcggg catacatcag atcgggaccg gtacccgagt caagctgaag
     5101 gcgaagcgtc gcgttgtagt cgggcgggtt tgtcggttga aagcgaattg ttacatccgg
     5161 tgcgaccttc ttgtactccg cgagcacccg attcatctgc tcaacgtcat ccgctcgcca
     5221 agagcccatc gtaagggtga ccgctgatgg agcttttgcg ccctgtgctg taagtggcag
     5281 taaaacaaga agtagaagca atacagcaaa aaagagtgtt tttttgttca tacggaactc
     5341 ctttttgtgt tttcagcgac agtataccat cttccgggat gtatttgcca aaaatctatt
     5401 aagtaattgt aaaattctag tgcctaaaaa ttctgaatgt ggtacggtgg ttagggaatt
     5461 caagtgaggt agatcatgaa cctaattttt cttggtcctc cgggagcagg caaagggacg
     5521 gttgccgacc tggcgaaaga cgtgctgggg atcccccata tttcaagcgg acagctgttc
     5581 agagagcaga tcaaaatcgg atctcccttg ggcaagcggg tggaagcgac gctcaaggcc
     5641 ggaaatttgg tggatgatga aacgacgatc gagttcgtcc gggagcgcct ttcccgcgat
     5701 gacgtggctc gaggatacat cctggatgga tttccccgga cgatcgccca ggccgaagcg
     5761 atgtgcgtct tctccaagcc ggaagcggtg atcaactttt cccttaccga agaagagatt
     5821 cttcgtcgcc tggagggaag gcgggtcgct cctacaacgg gaagaaccta tcacatcctc
     5881 tacaatccgc cgaaggttga agggcttgat gatgaaaccg gggaaacgtt gattcaacgg
     5941 ccggatgaca agaaggaagc gatcttgaat cgcttggaag tctacacgag agaaacccgg
     6001 cccttgatca atcactaccg cgaaaagaat ctcttggtgc ccctcgacgc ttcaccgagt
     6061 ccccaagagg ttctcaacca actgttgcac ctgttgaagg aaaagggcat ccactaaagg
     6121 aggttccatg gccacctatc acaatgagcc ctcgcgaacg ttcagcgagt atctcctgat
     6181 tccgggatat tccgatcgaa actgcgtccc ggccaacgtt tcactggcaa caccgcttgt
     6241 tcgttacagg aagggaatgg aagagtgtcc gctttccctt cagatacctc ttaccagtgc
     6301 gatcatgcaa tcggtgagcg gagagaaaat ggcgatcgcc ctcgcccggg agggtggctt
     6361 gtccttcatc tacagctccc aaccgattca ggatcaggaa gcaatggttc gacgggtgaa
     6421 aggatataaa gcgggctttg tcacctccga ctcaaacctc actcccaacc atactcttgg
     6481 cgatctgatc gccctcaaag aagaaaaagg gcactcgacg atcgcgatca cggacgatgg
     6541 aacgagcagc ggaacactgc tcggactcgt ctcatcccgc gactggcggc caagccgaac
     6601 aagtccggac
//
LOCUS       DAOPUZ010000011         6059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_12956_length_6059_cov_3.713358, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000011 DAOPUZ010000000
VERSION     DAOPUZ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12956_length_6059_cov_3.713358"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(463..1569)
                     /locus_tag="PKN79_00485"
     CDS             complement(463..1569)
                     /locus_tag="PKN79_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNZ94278.1"
                     /translation="MTRKEELRIKLDRVRTLMSSLEFDGVFLKRQDDFSWLSCGGQNY
                     IGWGDMGLCGLLVTQDKTYAVTNVIEEPRMREEEQLEELGFKIHAGAWQDTGFEARTL
                     KSLVPSGRIAFDYTHDLGPNIEGEIKKLRLSLTKSEIERMRKVGIMATLALEEAAVSV
                     RPGDTEIEAIKRIANALYDQDMMSTSLMCAADDRLMKYRHAIATPTKIKERFQIGGNM
                     RKWGLTVCLTRYVNFVPVTDELRKQYRLNQLIDTTMMVNTVVGRAYHEPLKAAQKLYD
                     EHGYGDEFLKHHLGGPIGYANRDYRVDFNLPGTIQENQGFCWNPSITGTKSEDTILVR
                     KDGFEFITRPIVFPKNEVVVDGKTFIRSDILEKF"
     gene            complement(1585..2877)
                     /locus_tag="PKN79_00490"
     CDS             complement(1585..2877)
                     /locus_tag="PKN79_00490"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNZ94279.1"
                     /translation="MAVTTGGSGSWRVIDSLLEGIPIPKMVKVHQNFFLPEVIDIQNA
                     LAAQLSSETLWAPLKRGDTVAIGIGSRGIDGQIEAVRTLVSAIKERGGVPFLVPAMGS
                     HAGATAEGQKAMLLTLGMDQVDAPIKSSMETVLLGRCPDGMPVLVDRLAHEADHLIII
                     NRVKSHVCFRGPYESGLVKMIAIGLGKQQGAETAHNLGFGRMPRHIEAIATEALSHLK
                     LLFAVALVENAAHRCSHIEVIAPDDLLTREPELLKLSKSWEARLHFETLDALVIDEIG
                     KHISGSGFDTNVVGRYHSEHITGGPKITRVAILDIASESNGNGNGLGMADFTTVRAVE
                     KFDFAQTYPNTLTALLTGGVKIPMVLMNDRQVFQACMKTSYRQRWEEARIVRIRNTLS
                     LGEIEVSESIVPEIEHDSRFEIIGEPYELQFNDEGNLF"
     gene            complement(2880..4358)
                     /locus_tag="PKN79_00495"
     CDS             complement(2880..4358)
                     /locus_tag="PKN79_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNZ94280.1"
                     /translation="MMQNLAYGFSLVFQPFTFALMVFGTAIGVVIGALPGLTASMGII
                     LLLPLVYRLPANVSLVMLCGLFCGGMYGGSISAILLNTPGTPSASATTLDGYPLNRQG
                     KAGKALGIATIASFGGGLISTACLIVIAPQLAKVALSFQAADYFSLSIFGLSIMASSS
                     GKNVVKGLIAGCIGLLASCVGIDTIVGVNRLTFNNMYLAGGFSLLPVLIGVFAVSEVL
                     KQVQTSHEERPVTIQAVTDILPTWTEIKGVIPAILVGGVLGVFIGIVPGTGGAIACFL
                     AYNVVQKFAKDRDQFGKGALSGVAAPESANNATTGGALIPMLCLGVPGDVVTSVMLGA
                     LVLIGVRPGPLLFIENIDVVYSIFAGMVIIQFLMLGFGLLSSRYSPAILKIPDTLLMP
                     VIMVLCVVGAFCLNNSLYDVTVALVFGILGFFMKRWGYPGAPLVLGVILGPMAEENLN
                     RALLVSNNNWAVLVQRPISLMFLLLSALSIGLALYNVKKKEA"
     gene            complement(4371..4829)
                     /locus_tag="PKN79_00500"
     CDS             complement(4371..4829)
                     /locus_tag="PKN79_00500"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HNZ94281.1"
                     /translation="MKDLKRVDLWVGVGLMALSIAVWLMTASLPVPERGIGPGQYPRV
                     ISVMMFGLGAIQIGTSLRGGLPERSSDRTYVTQIGRALLLAVISLIYVRLLNLIGFPL
                     LTPFFLVGIIRLFGYKKWKTNILVSVIGTAVIFVLFNIVFMVFLPVGRLF"
     gene            complement(4922..5896)
                     /locus_tag="PKN79_00505"
     CDS             complement(4922..5896)
                     /locus_tag="PKN79_00505"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HNZ94282.1"
                     /translation="MKKILTFLMILVLVTGTLFAQAQAETIDDFPSKPVTAVIGWSAG
                     GGADIVFRALAEVFPKYANGQPLVIKNEGAAAGVPAITAFKNATPDGYTIMHWNVAHL
                     IKTHMTKVPFTATEFEEACQVVASYNYLVVNADSKWKTLDEFLADARSKPEGISMGNA
                     GTGGGNHMAALLFEGATGTKYVHVPFSGGGPAITGLMSHQCDAVMAIAPEGISNVQGG
                     QLRILAVFSDKQIDGIPAKTGREQNIDLVLEQWRGVIAPTGTPPAILKKLENIFKQCV
                     ADPAYIAKMKELGATPVFKSGAEYSKIVEADDARYEKIVREGGFGDRY"
BASE COUNT         1428 a         1764 c         1499 g         1368 t
ORIGIN      
        1 ataccgcgat gctcagggcc atcagcccga cacctaccca gaggtcgact cgtttcagat
       61 ttttcatctt cccctccctc taaaaagaag aatcacatgg cttctcatat catccatgtg
      121 gacatatagg gttactctaa cacatcattt cattttgtca acttctgtta caaatcaatt
      181 aattgctgat agcaaactta tttcaggatt gagtgccggg aaactctacc caatatatgc
      241 ttataactaa ttatattaga acaagttagt ttccatttca tcaaccaaac gccttctgca
      301 gcaaccggaa aaacggaaga gacaaaacaa atcaggaaga aagaggataa cctccatcca
      361 tgggggagat tcttcttccg attattacta ccatgccgga cgaactaaaa aataccggcc
      421 acggaagacc ggtattatca ttcaatccat gcgtattacc agttagaact tctctaaaat
      481 atcgctccgg atgaaggttt tgccgtcgac caccacctcg ttcttgggga agacgattgg
      541 tcgggtgatg aactcaaatc cgtcttttcg aacaaggatc gtatcctcgc tcttcgttcc
      601 ggtgatcgag gggttccagc agaatccctg attctcctgg attgttcccg gaaggttgaa
      661 atcgacccga taatcgcggt tggcataccc gatcggtccg ccgaggtggt gcttgaggaa
      721 ctcatccccg tatccatgct catcgtacag cttctgtgcc gctttcagcg gttcgtgata
      781 cgcccgaccg acgacggtgt tgaccatcat cgtcgtatcg atcaactgat tgaggcgata
      841 ctgcttgcgc agttcatcgg ttacgggaac aaagttgaca taccgggtca gacagacggt
      901 gagaccccac ttgcgcatgt ttccgccgat ctggaagcgc tccttaatct tcgtgggggt
      961 ggcgatcgca tggcggtact tcatcagccg atcgtcggca gcgcacatca ggctcgtgga
     1021 catcatgtcc tgatcataga gcgcgttggc gatccgcttg atcgcttcaa tttcggtgtc
     1081 acccggacgg acggaaacgg cggcttcctc aagggcgagt gtcgccatga taccgacttt
     1141 tctcatccgt tcgatttcag atttcgtaag cgaaagacgg agttttttga tttccccttc
     1201 gatattcggc ccgagatcgt gggtataatc gaatgcgatc cgtccggagg gcacaaggct
     1261 ctttaacgtt ctcgcttcaa agccggtatc ttgccatgcc ccggcatgaa tcttgaaacc
     1321 cagctcctcc agttgctcct cttccctcat tctcggctct tcgatgacgt tggtgacggc
     1381 ataggtctta tcctgggtta ccaacagacc gcataagccc atgtctcccc agccgatgta
     1441 gttctggcca ccgcaggaga gccaggaaaa atcgtcttgc cgtttgagga acactccatc
     1501 aaactcaaga ctgctcatca gtgtcctgac tcgatccagt ttgattcgca attcttcttt
     1561 tctcgtcatt cttcttcctc cgtattaaaa tagatttcct tcatcattga attgaagctc
     1621 gtatggctcg ccgataatct caaagcggct gtcatgttca atctccggta cgatcgattc
     1681 cgatacctcg atctcgccca aggagagcgt gttccggatt cgaacgatcc gggcttcttc
     1741 ccatcgctgg cgataacttg tcttcatgca tgcttgaaac acctgccgat cattcatcag
     1801 gaccatcggg atcttcaccc ctccggtcag caaggcggta agcgtgttcg ggtaggtttg
     1861 agcgaagtcg aacttctcca ccgcccgaac ggtcgtaaaa tccgccattc ccaagccgtt
     1921 gccgtttccg ttggactcgg aagcgatatc caaaattgcc acccgggtga tcttcggtcc
     1981 gccggtgata tgctccgaat gatagcggcc cacgacattc gtatcgaacc cgctgccgct
     2041 gatgtgttta ccgatctcgt cgatgaccaa ggcatcgagc gtctcgaagt gaagccgggc
     2101 ttcccagctt ttcgatagct tgaggagttc aggctctctt gttaagaggt catcgggggc
     2161 gatcacctcg atgtgggaac atcgatgggc ggcattctca acgagggcaa cggcgaagag
     2221 gagcttcaga tggctcagcg cctcggtcgc gatcgcttca atgtggcgag gcatccgtcc
     2281 gaatccgaga ttgtgggcgg tctccgctcc ctgttgcttt cccagaccga tcgcgatcat
     2341 cttgaccagg ccgctttcat acggcccgcg gaagcataca tggcttttta cgcggttgat
     2401 gatgatcaga tgatccgcct cgtgggcgag gcggtcgaca agaaccggca ttccgtccgg
     2461 gcacctgccg agcaacaccg tctccatcga acttttaatc ggtgcatcga cctgatccat
     2521 gccgagcgtc agaagcatcg ctttttggcc ttcggcggtg gccccggcat gactacccat
     2581 cgccggaacg agaaacggaa ctccaccgcg ctcttttatc gcggaaacga gcgttcgcac
     2641 agcctcgatc tgtccgtcga taccgcggct tccgatcccg atcgctaccg tatcacccct
     2701 cttaagcggt gcccaaagcg tttctgaaga gagttgagcg gcaagcgcat tctgaatatc
     2761 gattacctcc ggaaggaaga agttctggtg caccttcacc attttgggga tgggaatccc
     2821 ttcaagtagt gaatcaatga cgcgccacga accgcttcca ccggtggtaa ccgccatccc
     2881 tacgcctcct tctttttcac gttatacaat gcaagcccaa tagaaagagc cgagagaagc
     2941 aggaacataa gtgaaatcgg acgctgaacc aatactgccc agttgttgtt ggagaccagc
     3001 agcgcccggt tgaggttttc ttcggccatc ggaccgagaa tcacccccag gacaagcggc
     3061 gccccgggat agccccagcg cttcatgaag aagcccagga ttccaaacac cagagcgacc
     3121 gtgacatcat acagggagtt gttcaggcag aaggcaccta cgacgcacaa caccatgatg
     3181 accggcatca gaagggtatc gggaatcttc aggatcgccg gtgaataccg ggagctcagc
     3241 agtccgaagc ccagcatcag gaattggatg atcaccattc cggcaaagat cgaatagacg
     3301 acatcgatgt tttcaatgaa gagaagcggt ccgggccgta ccccgataag aaccaaagct
     3361 ccgagcatga cgctcgttac cacgtctccg ggaactccga gacacagcat cggaataagc
     3421 gcaccaccgg tggtggcgtt gttcgccgat tccggggcgg cgacgccgct gagagcgccc
     3481 ttgccgaact gatcccggtc ctttgcgaat ttttgaacca cgttgtatgc gaggaaacat
     3541 gcgatcgctc caccggttcc gggaacgatt ccgatgaaca cccccaacac cccgccgacg
     3601 agaatcgcag ggatcacccc tttgatttcc gtccaagtcg gaagaatgtc cgtcaccgcc
     3661 tgaatcgtaa ccggccgctc ctcatgggag gtctgaacct gcttcaacac ttcactgacc
     3721 gcaaacacac cgatgaggac cggcagcaag ctgaaaccgc cggcaagata catgttgttg
     3781 aacgtgaggc ggttgacccc gacgatcgtg tcgattccga cacaggaggc cagcagtccg
     3841 atgcatccgg cgatcagacc tttgacgacg ttctttcccg atgaactggc catgatggaa
     3901 agaccgaaaa tactcaagga aaaataatcc gcagcctgaa atgacagggc gacttttgcc
     3961 agttgcggcg caatgacgat aagacacgcc gtactgatca agcctccgcc aaaggaagcg
     4021 atcgtggcga ttccgagagc cttgcccgct ttgccctgac ggttgagcgg atagccgtcc
     4081 agcgtcgtgg ccgaagcgga gggggtcccc ggagtattga gaagaatcgc cgaaatcgat
     4141 cctccgtaca tccctccaca gaagaggccg cacagcatga ccaacgagac attggcggga
     4201 agccggtaga ccagaggcaa caacaaaatg attcccatcg atgcggtaag acccggcaat
     4261 gcaccgatca cgacaccgat cgccgtcccg aataccatca gggcgaaggt gaacggctgg
     4321 aagaccaagg aaaatccata tgctagattc tgcatcatct cacacccccc ttagaagagc
     4381 cttcccaccg gaaggaacac catgaagacg atgttgaaca gaacgaagat gaccgccgtg
     4441 ccgatcacgc tcaccagaat attcgtcttc catttcttgt acccgaacag gcggatgatg
     4501 ccgacaagaa agaacggggt aaggagcgga aatccgatca gattgagcag tcgcacgtat
     4561 atcagggaga tcaccgccaa cagaagcgct cgccctatct gagtgacata cgtacgatca
     4621 ctgcttcgct cgggaagtcc gccgcgcaga ctcgttccga tttgtatagc gcccagcccg
     4681 aacatcatca cggaaatcac ccgcggatac tgtcccggtc cgatcccccg ttcgggaacc
     4741 ggcaagctgg ccgtcatcaa ccataccgcg atgctcaggg ccatcagccc gacacctacc
     4801 cagaggtcga ctcgttttaa atctttcatc atcccctccc tcctcccggt gaagacgtta
     4861 taaagatatt acaaagatgt tgctccgaac caaggcggag caacatcctc aaatcacgcg
     4921 atcaatacct gtcgccgaaa ccgccttccc gaacgatctt ctcatagcgc gcgtcgtccg
     4981 cttcgacgat ctttgaatac tctgctccgg atttaaacac tggcgtcgca cccaattcct
     5041 tcatctttgc aatgtaggcc ggatccgcga cacattgctt gaagatgttc tcaagcttct
     5101 tcaggatcgc cggcggagtt ccggtaggag cgatgactcc tctccactgc tccagaacca
     5161 ggtcgatgtt ctgttcacga ccggtcttgg cgggaatgcc gtcgatctgt ttgtcgctga
     5221 agaccgcaag aattctcagc tgaccccctt ggacgtttga tattccttcg ggagcgatgg
     5281 ccatgacggc atcacactga tggctcatca agccggtgat cgccggtcct ccccctgaga
     5341 aggggacgtg gacatacttg gttccggtcg ctccttcaaa gagcagcgcc gccatatggt
     5401 tccctccacc ggttccggca ttgcccatcg agattccctc gggcttgctg cgggcatcgg
     5461 caaggaactc gtcgagggtc ttccatttgg aatccgcatt caccaccaga tagttgtagg
     5521 atgcgacgac ctgacacgcc tcctcgaatt ccgtagccgt gaagggaact tttgtcatgt
     5581 gggtcttgat caagtgggcg acgttccagt gcatgatcgt atatccgtcc ggagtcgcgt
     5641 tcttgaacgc cgtgatcgca ggcactcccg ctgcggcacc ttcgttcttg atgaccagcg
     5701 gctgaccgtt cgcgtacttc ggaaacacct cggcaagcgc cctgaatacg atatccgcac
     5761 cgccaccggc tgaccagccg atgaccgccg taaccggctt gcttgggaaa tcgtcaatgg
     5821 tttccgcttg tgcctgggca aacagagtac ccgtaacaag aacgagaatc atcaagaatg
     5881 tcagaatttt cttcatctct ttcctcctaa aaagttgaat cacgcggctt cctcagccac
     5941 gcacctatac ggtcactcta acacatcaat ctattttgtc aacttctatt acaaattaat
     6001 ttttacccct tgcaatcccg ctttcagtgc cctatcattc atccatatgc agaacgacg
//
LOCUS       DAOPUZ010000012         5997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_13171_length_5997_cov_2.749243, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000012 DAOPUZ010000000
VERSION     DAOPUZ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13171_length_5997_cov_2.749243"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKN79_00510"
     CDS             complement(<1..210)
                     /locus_tag="PKN79_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ94283.1"
                     /translation="MEYKTFSNGVKIPVVGFGTWQVSPDEAYESVRSALEVGYRHIDT
                     AAAYDYEAEVGQAITDSGIRREEIFV"
     gene            315..387
                     /locus_tag="PKN79_00515"
     tRNA            315..387
                     /locus_tag="PKN79_00515"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:348..350,aa:Met,seq:cat)
     gene            complement(427..1785)
                     /locus_tag="PKN79_00520"
     CDS             complement(427..1785)
                     /locus_tag="PKN79_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNZ94284.1"
                     /translation="MHIVLIGAGSAQFGLGSLGDIMQSKLLAGSSIALVDIDEQALSR
                     VNAIAQAFIKEKGLNFTVTATTDRKAALKGADAVIISIEVGQRFPLWEEDWTLPQQYG
                     SQQIYGENGGPGGLFHALRITPVIVEMCDDVMEVCPDAWVFNYSNPMTAITTTVLRKY
                     PDLKFVGLCHEIASLERYLPSILNTPFENLELKAAGLNHFSVLLSARYKDSGKDAYPD
                     ILEKAPAFFEREPGYSDILAYLKAHPQELQTEGSTNRALPKEIRSRKSWADRGLFREI
                     LETYKLLPITVDSHIGEYISWANEVADHKGIKDFFELYQIMLGFKKTSIELKAHERIS
                     FILDGIVSNSGYEESAVNILNDQLIPDLPSWIAVEVPARVYAKGMEGIAFPDYPKGFG
                     ALLRNYTGVYDLTAEAILRKKKEYAIQALLASPITTPVRAASELVELMIQRQEKWLGY
                     LK"
     gene            1856..2380
                     /gene="thpR"
                     /locus_tag="PKN79_00525"
     CDS             1856..2380
                     /gene="thpR"
                     /locus_tag="PKN79_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNZ94285.1"
                     /translation="MRLFFGLTFSKEDKIALARVSGSIDPYVTKGKAVREDNYHLTLS
                     FIGEVEQKYLDVFCSILDRIDPPVLTLNMKRLGLFPSRRHNTLILHVEPDERLTVLQQ
                     TLEQDLVSKKLIAKGGKYKPHITLFRQTVIPIVPMIEPIEISPTSVALFHSHRENNLL
                     TYTVVCEKELGIDG"
     gene            2373..3167
                     /locus_tag="PKN79_00530"
     CDS             2373..3167
                     /locus_tag="PKN79_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ94286.1"
                     /translation="MDESRYIRYNEQAWNREVDRKSIWTDGLTEEELQKALSGTVEIR
                     LTTYKPMPSAWLGDLAGKRVLAVGSGGGQQAILFALAGSEVTLLDLSGKQLEQDRTMA
                     NRLGLELTLIKGDMRDLSQFDVEDFDIVFNPASTVFIDDVRSFYRECARVLKPKGVFL
                     TSITNPVLYLFDERLALKNKLKVKYTLPYSDIRSLSRKRIEKMIKTGDTFEFSHTLAD
                     LLGGLGDAGFIIEGVYSDRCGFELLDSFIADAYLAVRATKRLPHDS"
     gene            3186..4010
                     /locus_tag="PKN79_00535"
     CDS             3186..4010
                     /locus_tag="PKN79_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94287.1"
                     /translation="MENYLKKVLITKGLDDPIAFAKAQQRIAELEEKRIDSFNEFGPL
                     HERYLVINDLLLGAYENAGDTLNQIEAAETSYQALVHLLGKENQESSKAYIALGRAYL
                     QDGQIKEAQEVCDYVLKAAIGERDDYSWELYVDTLSLHADILFVKGNYHEELRYRKHL
                     YDIANQHFGIADERTVLLRVALAYCLEKLRRFKEASEHYVVVRAYMEQDRTFATDAEK
                     IGLTVHLARCYYMIGQKDDARQIYGWAYSLARRTYGPSSALTKKTLRILKHINRLG"
     gene            4153..4767
                     /locus_tag="PKN79_00540"
                     /pseudo
     CDS             4153..4767
                     /locus_tag="PKN79_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607396.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
     gene            4898..5902
                     /locus_tag="PKN79_00545"
     CDS             4898..5902
                     /locus_tag="PKN79_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNZ94288.1"
                     /translation="MIEERIIEQPKSGLVVLIVNTLAILFAIGVFVASIVLGVSSPSA
                     WLPIGLTVVSALYAFIIGPILYVGLKVLTKNEALVLTLFGRYYGTLKQDGFFFVNPFC
                     VGFNPTAGTNPSTGATREKKKEQIVVSPQGMSVDFKLSKKKISLKALTLNNDKQKIND
                     SLGNPIIIGVVVIWKVVDTAKAVFNVDNYIEYLSIQTDASLRDAVRRYPYDSEDDEKS
                     LRGSANEVAEDLKKALQKKVEVAGIEIIETRITHLSYAPEIAAAMLQRQQATAIIAAR
                     KKIVEGAVGMVEMALEQLSEKGVVDLDEERKASMVSNLMVVLSSNRDTQPVVNTGSIY
                     "
BASE COUNT         1418 a         1654 c         1576 g         1349 t
ORIGIN      
        1 aacaaagatc tcctcccgcc tgatgccgct gtcggtgatc gcctgaccga cttcagcttc
       61 atagtcatat gctgcggcgg tatcaatgtg ccggtatccc acctccaatg cggaacggac
      121 gctttcatat gcctcatccg gagagacttg ccacgttcca aacccgacga cgggaatttt
      181 gaccccgttg ctgaacgttt tatattccat ttcgttcctc acttcttcaa agtcctatcc
      241 taccacaaag gcaaatcatc ctcaaacccc cttgactgag ctaaagaggt ttggctatag
      301 taagctcgtt gtttggcggc atagctcagt tggtagagca agcggctcat atccgctagg
      361 tcaggggtcc gagtcccttt gccgctaaaa aggcctcccc gcatgttgag gaggcctctt
      421 tttttcttat ttcaggtacc ccaaccactt ttcctgacgt tgaatcatca actccacgag
      481 ctcgctcgca gctctgacgg gtgttgtaat cggactggcg aggagtgcct ggatcgcata
      541 ctccttcttc ttcctgagga tcgcttcggc agtcaggtca tacactcccg tgtagtttct
      601 aagcagcgct ccgaacccct tcggataatc ggggaacgcg attccctcca tccctttggc
      661 atagacgcgg gcgggcacct cgactgcaat ccaggaagga agatccggaa tcagctgatc
      721 gttgagaatg ttcaccgcac tctcctcata cccggagttg gagacgatcc cgtccagaat
      781 gaaggagatc cgctcgtggg ccttgagctc aatgctggtc tttttgaaac cgagcatgat
      841 ctgatagagc tcgaagaagt ccttgatgcc cttgtgatcg gcgacttcat tggcccagga
      901 gatatattca ccgatgtgcg aatcgacggt gatcggaaga agcttgtagg tttcaagaat
      961 ctccctgaac aagccccggt ccgcccagct cttcctgctt cggatctcct tgggaagagc
     1021 ccggttggtc gagccttcgg tctgcagctc ctgcggatgt gctttgaggt atgcaagaat
     1081 atcgctgtag cccggttcgc gctcgaagaa agcgggcgct ttttcaagga tatcgggata
     1141 ggcgtccttg ccgctgtcct tgtagcgggc gctgagaagg acactgaagt ggttcaaccc
     1201 cgccgctttg agctcgaggt tctcaaacgg cgtattgaga atggagggaa gataacgctc
     1261 aagcgaggcg atttcatggc agagccccac gaacttcaga tccgggtatt tcctgaggac
     1321 ggttgtggtg atcgccgtca tcgggttcga gtagttgaac acccacgcat cgggacagac
     1381 ttccatcacg tcgtcacaca tctcaacgat gaccggggtg atcctcaggg cgtggaagag
     1441 tccgccgggg ccgccgttct caccatagat ctgctgtgat ccgtactgct ggggaagggt
     1501 ccaatcctcc tcccagaggg ggaagcgctg gccgacttcg atcgagataa tcaccgcatc
     1561 agcgcctttg agggcggctt ttcggtcggt ggtcgccgtt acggtgaagt tcaatccctt
     1621 ttctttgatg aacgcctggg cgatcgcatt cactcgcgaa agcgcttgtt cgtcgatatc
     1681 aaccaatgcg atcgaagagc ctgcgagcag cttgctctgc atgatgtccc ccaaggaccc
     1741 taaaccgaac tgggcgcttc cggccccgat taaaacaatg tgcatatgag cctccttccc
     1801 cactatacac caacgagaat gttttattcc atcgatcatc gtgtatactc taggtatgcg
     1861 actctttttt ggtttgacat tctcgaaaga agacaagatc gctctcgcac gggtaagcgg
     1921 atcgatcgat ccgtatgtca ccaaaggaaa ggcggttcgc gaggacaact accacctcac
     1981 cctctccttc atcggcgaag tcgaacaaaa gtatctggat gtgttttgtt cgatcctcga
     2041 tcggattgat ccgcccgtcc tcacgctgaa catgaaacgg ctcgggctct tcccttccag
     2101 aagacacaac acccttattc ttcacgtcga acccgatgaa cggcttaccg tcctgcagca
     2161 gacgcttgaa caggacctcg tctccaaaaa actcatcgcc aaagggggga aatataagcc
     2221 gcacatcacc ctctttcgcc aaaccgtcat tccgatcgtg ccgatgatcg aaccgatcga
     2281 aatctctccg acctcggttg ctttgtttca ctcccatcgg gaaaacaatc tcttgacgta
     2341 taccgttgtc tgtgaaaagg agcttggaat cgatggatga gagccgatac atccgctaca
     2401 acgaacaagc atggaaccgc gaggtcgata gaaagagcat ctggacggat ggcctgaccg
     2461 aggaggaatt gcaaaaagcg ttgtcgggaa cggtcgagat ccgacttacc acgtacaagc
     2521 cgatgccttc cgcctggctt ggagacctcg cgggcaaacg ggtgctcgca gtcggatccg
     2581 gcggcgggca gcaggcgatc ctgttcgctt tggccggcag cgaggtcacg cttctcgatc
     2641 tctccggcaa acagctcgag caggaccgaa cgatggccaa tcgattgggc ctcgagctga
     2701 ccttgatcaa gggtgatatg agggatcttt cacagtttga tgtggaggat tttgacatcg
     2761 tcttcaatcc cgcttccacg gtgttcatcg atgacgtccg ctccttctac cgggaatgtg
     2821 cccgcgtgct caaacctaaa ggagtctttt tgaccagcat caccaatccc gtcctctatc
     2881 tcttcgatga acgactggcc ctcaaaaaca agctgaaagt caaatacacc cttccctaca
     2941 gcgatatccg gagtttgagc cggaaacgga tcgagaagat gatcaagacc ggtgacactt
     3001 tcgagttcag ccacaccctt gccgatctgc tcggcggcct tggggatgcg gggtttatca
     3061 tcgaaggggt gtatagtgat cgatgcggct tcgaactgct tgattccttc atcgccgatg
     3121 catacttggc ggtccgggct acgaaacgct tgccccatga ctcgtagctt gggtattata
     3181 caggtatgga gaactatctg aagaaagtcc tgatcaccaa aggcctcgat gacccgatcg
     3241 ccttcgccaa ggctcagcag cggattgccg agctggagga gaagcggatc gattcattca
     3301 atgagttcgg tccgctccat gagcggtatc tggtgatcaa cgacctgctc ctcggggcgt
     3361 acgagaacgc cggcgatacg ctcaatcaga ttgaagccgc cgaaacctcc tatcaagccc
     3421 tcgtccacct gcttggcaaa gagaatcagg aaagttcaaa agcatatatc gccctcggcc
     3481 gggcgtatct gcaggatgga cagatcaaag aagctcagga agtgtgtgat tatgttctca
     3541 aggcggcgat cggggaacgg gatgattact cttgggagct ctatgtcgat accctcagcc
     3601 tccatgccga catcctcttt gtcaaaggca attaccacga ggaactccgt tatcgtaagc
     3661 atctctacga catcgccaat caacacttcg gaatcgccga tgaacggacc gtgctcttgc
     3721 gggtagccct cgcctactgc ttggaaaagc tccgccgctt caaggaagca agcgaacact
     3781 atgtcgtcgt cagagcctac atggagcagg atcggacgtt cgcaaccgat gcggagaaga
     3841 tcggtctgac cgtccatctg gcccggtgct actacatgat cggacaaaag gacgacgcaa
     3901 gacagatcta cggctgggca tactccctcg cccgtcgaac ctacggtccc tcctccgctc
     3961 ttaccaagaa aacgcttcgc attctcaaac acatcaatcg actcggctga gagtctcctg
     4021 ttcccttttg caccaagcaa tgaaaagtca atggatgacg gtttgaacgc ttgcctcaca
     4081 ggggttcctt gagtatcttt agctaaagtt agcaataggc aacttatcat aactctttat
     4141 ggaggtatca ccatgttcgt aaaatataca ttgccctatt cctatgatgc gatggagccg
     4201 cacattgatg cgctcacgat ggagactcac tacaccaagc accatgaagg atatacgact
     4261 aacctgaacg atgcggtcgc aaaagcccaa gtggacaagg acattgcatc tctgctgggc
     4321 tcgttggatg agatcgccga tcccgccttg cgtcggacga tccgaaacaa cggcggcgga
     4381 tactataacc acaacctcta cttcgcaacg atctctccgg atgggggaaa agatccctca
     4441 ggggcgttcg ggaatctgct gaagagcgaa ttcggaagta tggacgagct gaaagccaaa
     4501 ctgagcgcgt tcgctctcgg tcagttcggt tccggctggg cgtggctcag tgcaaacaag
     4561 gatgggaagc tcctcctctc ctcgaccccc aaccaggaca accccctgat ggacggaaaa
     4621 ggctttaccc cgattctcgg catcgacgtc tgggaacatg cctactacct caagtagaaa
     4681 aaccttcgag gcgagtacgt gaaggcgttc ttcaacgtcg tcgattggga tgaggtggcc
     4741 gggaattacg agaagatcag aaaataagcg cttcccatca acacaatgac ggtacagtcg
     4801 atccggctgt accgtttttc tgttttcggg ggttctttca tcttgtacat attttatcat
     4861 cgtgatatcg ttatgatatc agataaggag actctgcatg atagaagagc gaatcattga
     4921 acaaccgaaa agcggactgg tcgtcctcat cgtgaacacc cttgcgattc tcttcgccat
     4981 cggggtgttc gtcgcatcga tcgtgctggg agtgtcatct ccctccgctt ggcttccgat
     5041 cggattgacg gtcgtcagcg cactctacgc gttcatcatc ggcccgatcc tgtatgtggg
     5101 gctgaaagtg ctgacgaaga acgaggcgct cgtcctcacc ctcttcggcc ggtattacgg
     5161 aaccctcaag caggatggct tcttcttcgt caatccgttc tgcgtcgggt tcaacccgac
     5221 cgccggaacg aatccttcca ccggagcgac gagagaaaag aaaaaagaac agatcgtggt
     5281 gtcccctcag ggaatgagcg tcgacttcaa gctttcgaag aagaagatca gcctcaaggc
     5341 gttgacgctg aacaacgaca agcagaagat caacgacagc ctgggaaatc cgatcatcat
     5401 cggcgtcgtg gtgatctgga aggtggtcga caccgcgaaa gcggtcttca acgtcgacaa
     5461 ctatatcgaa tacctttcga tccagacgga tgcttcgctt cgtgatgcgg tccgtcgcta
     5521 tccctacgac agcgaggatg atgagaaatc ccttcgaggg tcagcaaacg aagttgccga
     5581 ggatctgaag aaggcgcttc agaagaaggt cgaagtcgcc ggaatcgaga tcatcgagac
     5641 caggatcacc cacctctcct acgccccgga gatcgccgcg gcgatgctcc agcgccagca
     5701 ggccacggcg atcatcgcag cgcggaagaa gatcgtcgaa ggtgcggtcg gcatggtcga
     5761 gatggcattg gagcagctca gcgagaaagg cgtcgtagac cttgatgaag agcgaaaggc
     5821 ttcgatggtc agcaacctga tggtcgtcct ttcctccaat cgggacaccc agccggtggt
     5881 caacacgggt tctatttact agtgatgaaa gaacaggaac ggaaacaggt attactccga
     5941 ctcaacggcg agctttggaa agagctcgcc cgctgggccg aagacgagtt cagatcg
//
LOCUS       DAOPUZ010000013         5871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_13532_length_5871_cov_3.217160, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000013 DAOPUZ010000000
VERSION     DAOPUZ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13532_length_5871_cov_3.217160"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1018
                     /locus_tag="PKN79_00550"
     CDS             116..1018
                     /locus_tag="PKN79_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ94289.1"
                     /translation="MRKKKQVSAIAASTKGDKVFDFLVNLFLVFMLVVIAVPMWSTIT
                     LSFRPNAYIGSNLQGMFLAPWKWSTAAYRALLGNDGFLMAFKNSFKILIGGVATSLFL
                     TIPLAYVLSIQSLPGRRYLTILIIIPYIFNVGLIPTYLLVTKLGLIDKIMAVFLPGAV
                     STYNTLIMRSFFEGIPNELKESARIDGASEIRILVMIILPLSKAIIMTIGLFYGVGFW
                     NDFFRAMLYLNSNKLQPLPILLRNILMASGMNEFVEISAFGDAPIEAIKAASVFMSAI
                     PMIVAYPFIQKYFTKGTLLGSVKG"
     gene            1040..2617
                     /locus_tag="PKN79_00555"
     CDS             1040..2617
                     /locus_tag="PKN79_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ94290.1"
                     /translation="MRKQMSTWLVLVTILALVVMPLFSQGAADKKASEGTTVIELWYG
                     AAMTEAGPPPEDWVGFQIIKDKLNIDLKITALPSSEGDQDVKIQAAAAANNLPDLFMV
                     RREVLARLVPQGLVAPVDDLYAKMPVRTANHYDEVSRRNAMFNGKSYGLSDPGAIIKN
                     EGLLIRKDWLDNLGLAVPTTIEELFTVMRAFTFNDPDRNGKHDTWGYGSFEEIGSYEG
                     WPGRRLQPIFGAFGAEGTWDMTEANAGLSVFKDGFYDAMVYLKRMVDEGVIDPNWMSY
                     KKDDFRAAWKQGRFGIMREQNAAYAAQSNYAPFDQNFPDGQWIIIDPPKGPKGHASVG
                     PYTANFRVYAISAKAAQAGKKDKIAELLEWMASDEGYYLLGWGVEGVNYTLDANGIPV
                     AAGVPDPDYSFTGPKGQVVTQLRNMVFYNGDVELYARYPTYTTAVSKKQMSALDVLRE
                     MQTKKWTPAVGSNTLPTPNADLKRFYEQSLSEFITGKRTLNRANWNAWLEEFRKLGGQ
                     AWNDAGVASMKENNLLN"
     gene            2674..3768
                     /locus_tag="PKN79_00560"
     CDS             2674..3768
                     /locus_tag="PKN79_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HNZ94291.1"
                     /translation="MAKNYSLEMAKSVINRYQPVQMQWHYEHGLVITAILEVGEHYRQ
                     TSFFDWAYSMYDPFIKEDGTIKNYRAGEYNLDMINAGRNLFLLHEKTGERRFIKAAHI
                     LREQLIGQPRTHSGIYWHKQIYPWQVWLDGVYMQGPFSALYAKYVDQREIIEDLAIQI
                     ERIYATLRDSKTGLLYHAWDESRGMRWSDLETGLSPHFWGRALGWYAMAVVDIIAIIS
                     TDDPLSDPLKKILNTLMGDILAFQQPDGMFYQVVDRSEAEGNYKETSASAMFAYTLLK
                     MARLGIGADPKIQKSALKALDGIEKRYLSVDEDGEYHLEGICSVAGLGGNPYRDGSLY
                     YYFKEPQVADDFKGVGPYILATLERETLST"
     gene            3940..5016
                     /locus_tag="PKN79_00565"
     CDS             3940..5016
                     /locus_tag="PKN79_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ94292.1"
                     /translation="MKKLFVLLCVLLVLSFSLFAQGTKDAGKATLRVGMVTDAGTIDD
                     KSFNQGTWEGILRAQKDFSLDVKYLKPAGTTEADYLKEIGNLVDAGYTMIICPGFKFE
                     TALFEAQDKYPNTKFVLLDGEPHSADYSVFRIEKNVVGLYWAEHQSGFLAGLAAALQI
                     KNGSFGFIGGMEIPAVQKFNWGFQQGVRYANANYKTNIRMDQVNNLYQGSFDNISAGQ
                     QIAAAMYDRGVDVIFAAAGGVGVGVINEAKNRGSGGQKVWVIGVDVDQYAEGLMPNGK
                     SIILTSAMKYLDRASYDMIVDELNGRFPGGQVLHLDATNDGVGIPVENPNLDKSVTDE
                     VAKVFAKLKSGEVKVADKGDGLFK"
     gene            5084..>5871
                     /locus_tag="PKN79_00570"
     CDS             5084..>5871
                     /locus_tag="PKN79_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ94293.1"
                     /translation="MNHVIEMLNIRKEFPGIVANDNITLQVREGEIHAILGENGAGKS
                     TLMSILFGLYQADEGTIKVRGKEVQIRDPNDANDLGIGMVHQHFQLVHNFTVTENIIL
                     GKEGGFILHRKSASNRIADLSARYGLNIDPDMVIEDITVGMQQRVEILKMLYRDAEIL
                     IFDEPTAVLTPQEIDELMQIMRNLVKEGKSVILITHKLAEIKAVADRCTIIRRGKLID
                     VVDVAKTPVSQMASMMVGRPVSFKVEKKPAQVGRVILDIHDLTV"
BASE COUNT         1419 a         1546 c         1637 g         1269 t
ORIGIN      
        1 actcgccacc gccgtcggac tcttcaaggg cgcaatcgga atgttgttga ttctcttcac
       61 caaccgcatc gtcaagcggc ttggtggctc atcactctac taggaggtgg ttcgtatgcg
      121 caagaaaaaa caagtatcag caatcgcagc ctccaccaag ggagataaag tctttgattt
      181 cctcgtcaac ctcttcctgg tcttcatgtt ggtcgtgatc gcagttccca tgtggtcgac
      241 gatcaccctt tccttcagac cgaatgcgta tatcggcagc aacctccagg ggatgttcct
      301 tgctccgtgg aagtggtcga ccgccgccta cagagcgctg cttggaaatg acggatttct
      361 gatggcgttc aaaaactcct tcaagatcct catcggaggg gtcgcaacca gcctctttct
      421 taccattccg cttgcatatg tgctttcgat ccagagcctg cccggacgcc ggtatctgac
      481 gatcctgatc atcatcccgt acattttcaa cgtcggactg attccgacct accttctggt
      541 gacgaaactc ggcctcatcg acaagatcat ggcggtgttt cttccgggag cggtcagcac
      601 ctataacacc ctcatcatgc gctccttctt cgaggggatt ccgaatgaat tgaaggagtc
      661 ggcccgaatc gacggggcga gtgaaatccg cattctggtg atgatcatcc tccctctttc
      721 aaaggcgatc atcatgacga tcggcctctt ctatggggtc gggttctgga acgatttctt
      781 ccgggcgatg ctctacctca acagcaataa gcttcagccg ctgccgattc tgctgcgcaa
      841 catccttatg gcgagcggca tgaacgaatt tgtggaaatc agcgcgttcg gcgatgctcc
      901 catcgaggcg atcaaagcgg caagcgtctt catgagcgcg attccaatga ttgttgcata
      961 cccgttcatc caaaaatact tcacgaaggg cacgttgctc ggaagtgtca aaggatgatg
     1021 tggaataagg aggttttcga tgagaaaaca aatgagtacg tggttagtcc ttgtgacgat
     1081 cttggctttg gtggtgatgc cgctcttcag tcagggagct gcagacaaaa aggcaagtga
     1141 aggaacgacc gtcattgagc tgtggtacgg tgcggcgatg acggaggcag gacctccccc
     1201 cgaggactgg gttgggtttc agattatcaa agacaaactc aacatcgatt tgaagatcac
     1261 cgcccttcct tccagcgaag gcgaccagga tgtgaagatc caagctgcag cggcggcgaa
     1321 caatcttccc gacctcttca tggtgcgccg ggaggtgctt gcccggctcg taccccaagg
     1381 gctggtagcc ccggtcgatg atctctatgc gaagatgccg gttcgaacgg cgaaccacta
     1441 tgacgaagtc agccgccgaa acgccatgtt caacggaaag agctacggtc tttccgatcc
     1501 cggagcgatc atcaagaacg aaggattgct gatcaggaag gattggctgg acaatctcgg
     1561 gcttgcggtt cccaccacca ttgaagagct gtttaccgtg atgcgggcgt tcaccttcaa
     1621 cgatcccgat cggaacggga aacacgacac gtggggctac ggcagctttg aagagatcgg
     1681 aagctacgag ggatggcccg ggcggcgcct gcagccgatc ttcggcgctt tcggtgccga
     1741 gggcacgtgg gacatgacgg aggcgaacgc cggactgtcg gtcttcaagg acggcttcta
     1801 cgatgcgatg gtctacctga agcggatggt cgacgaaggt gtgatcgatc ccaactggat
     1861 gagctacaag aaggacgact tccgagccgc ctggaagcaa ggacgattcg gaatcatgcg
     1921 cgagcagaac gcggcgtatg cggctcagtc caactatgct cccttcgatc agaacttccc
     1981 ggacggacag tggatcatca tcgatcctcc gaagggcccg aaaggccacg cctcggtcgg
     2041 accgtacacc gccaacttcc gcgtctatgc gatcagtgcg aaagccgccc aagccggcaa
     2101 gaaggacaag atcgccgagc tcttggagtg gatggccagt gatgaaggat actaccttct
     2161 tggctggggt gtcgaagggg tgaactacac cttggatgca aatggaattc ccgttgccgc
     2221 cggtgttccc gatcccgact acagcttcac cggaccaaag ggtcaagttg ttacccagct
     2281 gcgcaacatg gtcttctaca acggcgacgt cgagctttat gcccgatatc cgacctatac
     2341 caccgcggtg agcaaaaagc agatgagcgc cctcgacgtg ctgagggaga tgcagaccaa
     2401 gaagtggacc cccgccgtgg gctccaatac cctgccgacc cccaatgccg acctgaagcg
     2461 cttctacgag caatcgctca gtgagttcat caccggcaag cgcacgctga accgggcgaa
     2521 ctggaacgcc tggcttgaag agttcaggaa gcttggcgga caagcatgga atgatgccgg
     2581 cgtggcatcc atgaaagaaa acaatctctt gaactaggag atgtgccatg agtccggccg
     2641 attcggtcgg actcgtgctc ttggaattgg attatggcaa agaactactc cttggaaatg
     2701 gctaaaagcg tcatcaatcg gtatcaaccg gttcaaatgc agtggcatta tgagcatgga
     2761 ttggtcatca ccgcaatctt ggaggtgggt gagcactatc gacaaacatc cttcttcgat
     2821 tgggcgtatt cgatgtatga cccgttcatc aaggaggatg gaacaatcaa aaactatcga
     2881 gcgggcgagt acaacctcga tatgatcaat gccggacgga atctttttct cctgcacgag
     2941 aaaaccggag aacggcggtt catcaaggcg gcccacatcc ttcgcgaaca gctgatcggc
     3001 cagccgagga cgcacagcgg gatttactgg cacaagcaga tctatccgtg gcaggtctgg
     3061 ctggatggag tctatatgca ggggccgttc tccgctctct atgcgaagta tgtcgatcag
     3121 cgtgaaatca tcgaagatct cgcaatccaa attgaaagga tatatgcgac acttcgggat
     3181 tccaaaaccg gactgctcta ccatgcatgg gacgaatcgc ggggaatgcg ctggtccgac
     3241 ctcgaaacgg ggctttctcc ccatttctgg ggaagggcgc tcgggtggta tgcgatggcg
     3301 gtcgttgaca tcatcgcgat catttcaacc gatgatccgc tttccgatcc gctgaagaag
     3361 atcctcaata cgctgatggg cgatatcctg gcattccaac agcccgacgg gatgttctac
     3421 caggttgtcg accgaagtga agctgaaggt aactacaagg agacttcagc gagcgcgatg
     3481 ttcgcctata cgcttctgaa gatggcgagg ttgggaatcg gcgccgatcc caagattcaa
     3541 aaatccgccc tcaaggcgct ggacgggatc gaaaagcgct atttgagcgt tgatgaagac
     3601 ggggagtatc atctcgaagg tatctgcagc gtggcgggtc tcggaggaaa tccctatcgg
     3661 gacggttctc tctactacta tttcaaagaa ccgcaagtcg ccgatgactt caagggtgtc
     3721 ggcccctaca ttctggcaac cttggagcgc gaaacgcttt caacgtaacg attggatgat
     3781 cttcgccctg ggaggggatt ctgctttccc ctataaagca ccctccgcgg ggcgatattt
     3841 atatgggtct tctccttctt taatttgaca tctgacatac acccttgtaa aatgaatcta
     3901 cgtgtaaaga tcttatgaga tccaataagg agtagaagca tgaaaaaatt gtttgtattg
     3961 ctctgtgtcc tcttggtgtt gagtttctcg ctctttgccc aaggaacaaa agatgcggga
     4021 aaagccaccc tcagagttgg aatggtaacc gatgccggaa ccattgatga caaatccttc
     4081 aaccagggaa cctgggaagg aatccttcgc gcccaaaagg acttcagtct ggatgtgaag
     4141 tacctcaagc cggccggaac caccgaagcc gattacctca aagagatcgg caaccttgtc
     4201 gatgccgggt acacgatgat catttgcccg ggcttcaagt tcgagacagc cctgtttgaa
     4261 gcacaggaca agtatccgaa tacgaagttt gttctcctcg acggcgagcc gcacagcgcc
     4321 gactattccg tcttccgcat tgagaagaac gttgtgggtc tctattgggc ggagcaccaa
     4381 tccggcttcc tcgccggtct ggctgcagcg ctgcagatta aaaacggcag cttcgggttc
     4441 atcggcggaa tggagattcc cgccgttcag aagttcaact ggggcttcca gcagggagta
     4501 cgctacgcaa acgccaacta caagaccaat atccggatgg atcaggtgaa caacctttat
     4561 cagggatcct tcgacaatat cagtgccggt cagcagatcg ccgccgcaat gtacgaccgc
     4621 ggtgttgatg tgatctttgc agccgccggc ggcgtcggtg tcggtgtcat caatgaagcc
     4681 aaaaaccgcg gaagcggcgg acagaaggta tgggtgatcg gcgtcgacgt cgaccagtac
     4741 gctgaaggtc tcatgcccaa cggcaagtcg atcattctca ccagtgcgat gaagtatctc
     4801 gaccgggcat cctacgacat gattgtggac gagctcaacg gcaggttccc cggcggtcag
     4861 gttctccacc tcgacgcgac gaacgacggt gtcggaattc cggtggaaaa cccgaacctg
     4921 gacaagagcg tcacggatga agtggccaag gtcttcgcca aactgaagag cggcgaggtc
     4981 aaggttgccg acaagggcga cggcctgttc aagtaattga aggtatcttt gtgtatgggc
     5041 cacctttcct ggggtggccc ttctcctcaa tccgggggtt gtaatgaacc atgtaattga
     5101 aatgctgaat attcgcaagg agtttcctgg aatcgtggcg aatgacaata tcacgctcca
     5161 agtccgcgaa ggggaaatcc atgcgattct gggtgagaac ggagcgggaa aatcaacgtt
     5221 gatgagcatc cttttcgggc tctaccaagc cgatgaaggg acgatcaagg ttcgcggaaa
     5281 ggaggtccag atcagggacc ccaacgatgc gaacgacctg ggaatcggca tggtgcacca
     5341 gcacttccag ttggttcaca acttcaccgt cactgaaaac atcatcctcg ggaaagaggg
     5401 cgggtttatc ctccaccgaa aatccgcatc caatcggatc gccgaccttt ccgcccggta
     5461 cggcctcaat atcgatccgg atatggtcat cgaggacatc accgtcggga tgcaacagcg
     5521 ggtggagatc ttgaaaatgc tctatcggga tgcggagatt cttatttttg acgagccgac
     5581 ggcggtgctc actccgcagg agatcgatga gctgatgcag atcatgcgca acctcgtcaa
     5641 agaaggaaag agcgtgatcc tcatcaccca taaattggcg gaaatcaagg cggtcgccga
     5701 ccgttgtacg atcattcgac ggggaaagct catcgatgtg gtcgatgtcg caaagactcc
     5761 cgtgtcgcag atggcctcaa tgatggtcgg ccgaccggtg agtttcaaag ttgaaaaaaa
     5821 accggcccaa gtcggacggg tcatcttgga tatccatgat ctcaccgtca t
//
LOCUS       DAOPUZ010000014         5605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_14426_length_5605_cov_3.543964, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000014 DAOPUZ010000000
VERSION     DAOPUZ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14426_length_5605_cov_3.543964"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..862
                     /locus_tag="PKN79_00575"
     CDS             20..862
                     /locus_tag="PKN79_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNZ94294.1"
                     /translation="MAKFVPYKNYFYNRLHGMSSLVVASVGTEQSSPGFVHGPEVRTF
                     HTLHFCLKGSGVLRMNTLEHDVQVGDLMLIYPKMKVYPKADRKNPWELIWVGFNGADA
                     RLLTSAIGLSPSSPVVHTNPLSRQRIKQAFDAVYNARGDRPSQIVAMTGALYTLLSLL
                     MELSGTPFPYTSGQEYIDPACDFIALNFNRSITVEDIARHVGVSRSCLYRAFINNLSQ
                     SPQEYLTSYRIKFSCSLLEQSNLPLKEIAYRCGFSSSLYYSKVFKSVMGVPPSEYQMR
                     SNPE"
     gene            complement(864..1604)
                     /locus_tag="PKN79_00580"
     CDS             complement(864..1604)
                     /locus_tag="PKN79_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNZ94295.1"
                     /translation="MSISIFIADSPQQLGKKAAKKIVELLQAAIKEKGEARIILSTGA
                     SQFDTLEALLQSDVDWSKVEMFHLDEYVGLPETHPASFRKYLKERFVSKVNLKAAHFV
                     DGEGDIEGKRAKLSAIIKEKQVDVGIVGIGENGHIAFNDPPADFEATDAYIVVELDER
                     CKQQQVSEGWFATLNDVPKTAVTMSPKQIMSCKHIVSAVPYAVKAEAVYNTITSKVDR
                     MIPATLLKTHPDWNLFLDQESAAKLLAR"
     gene            complement(1607..2800)
                     /gene="nagA"
                     /locus_tag="PKN79_00585"
     CDS             complement(1607..2800)
                     /gene="nagA"
                     /locus_tag="PKN79_00585"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNZ94296.1"
                     /translation="MHLFINATLVLEDSLVHDGYLLEEDGVIKAFGPMPAPSFDGKVI
                     DCKGLYLSPGFVDIHTHGSGGYDYMDGSVEAFVQAAKAHMKFGATTILPTTLSSTDEH
                     LYRSFKDFKAAKATSKGMPHLPGLHLEGPYFCPKEKGAMEERHLRVPDPRHYMPILEA
                     GEGLIRRWSFAPELPGAYEMADVLKKEGIYLSAAHTCANYDQMLKAVDHGVELLTHFY
                     SGMSTITRVGGFRVLGVIESGYLIDGLKLEIIADGLHLPPELLQLIYKCKDADSICAT
                     TDSMRAAGLPEGPSILGPLVGGTDVIVEDGIAKMPDKTVFAGSVCTTDRMVRTLMEHV
                     GLNLPKAVKAASLLPARFVGLDSLTGSIAVGKQADLIIFNEDITIQSVFVSGTNTHIL
                     TDKEE"
     gene            complement(2891..4486)
                     /locus_tag="PKN79_00590"
     CDS             complement(2891..4486)
                     /locus_tag="PKN79_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94297.1"
                     /translation="MNYQQLTEAERITLNTSMFKAYDIRTKSEHVSKELSIRLLEAIA
                     RYYHEILSVDTIVLGRDARLAAPRLMEEALEIFPTFGLDVIVNPLQSSTCQFYFSCMQ
                     HPSAGGIMFTASHNPGEYIGLKLVAPPLQALALGAGPKGGITAIRDFYAAGSNKPSTK
                     TRGRVYIRRYLDQFIDYSRSLAGVHETTLKGVPIMVDFLSGAAGTEVAEALIRSGADL
                     TVRNLIPDGTFPAGEPNPIALESLRPTTELMKKGDWAFGFAFDGDGDRMDLMDRHGVQ
                     VPPSFNLSIIIPEVRSYFESVHAQGYWGSAPFNPQLFYDVKANPLSIVLQAKAGMGVH
                     IIRNGHSFIKEALRKNLDDQYVLASEESAHYYMNFPYDLNDLSKGFASTENTLFFALL
                     TARMYSQYPEKYEQAMKLQHSIHRRREWPCHFFDDKHLKPVVSEVERIFADRGLTVFK
                     EMEDGSSLDATLMRKGLPLKIDESSDLEGSWLQVAQRISRSEEGIARWEVASSTKEGL
                     DEAVGLIMAVTDRYVERGEAKYI"
     gene            complement(4464..>5605)
                     /locus_tag="PKN79_00595"
     CDS             complement(4464..>5605)
                     /locus_tag="PKN79_00595"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNZ94298.1"
                     /translation="YTNDGKTLVSRDKYTWSQGRMLWVLSYLLGSPTLARLLNEEERS
                     RYTERARLLHMFLDRHAFLDQADEGCAFLLGWDGTVKQGYTSIYADCFVIIGFSRYAR
                     FVESRSIVLKALALYTKIRGFIARGMIKSEPYPIPSSARSHGISMIMVNTADELSKAL
                     AELGLNDQAQTVAEDAQEDAMTILDRFVDPKTALVREIVHTDGNEESLLSRHRNPGHA
                     VESMWFCLNVLEDHLPRMGEVVLASLERGWDEEHGGLFRYIDSSGSPPQGKTQADLFS
                     RLVTSTWDYKLWWPHAEAVYATWRFYRATGNEVFLAWHRRLVSYTFETFPAETGNEWI
                     QIRNRSGQPVEGVVALPVKDPFHILRTVMYMTEDEEKTDELPTID"
BASE COUNT         1245 a         1578 c         1436 g         1346 t
ORIGIN      
        1 cttcttgtta tcgtacagta tggctaaatt cgttccatac aaaaactact tctataatcg
       61 cctgcacggc atgtcttcgc tcgtggttgc ctcggtggga accgagcaga gttcgccggg
      121 gtttgtccat ggcccggaag tacgcacctt ccataccctc cacttctgcc tcaagggatc
      181 gggagtactg agaatgaata ccttggagca tgatgtgcaa gtcggcgacc tcatgctgat
      241 ctatcctaaa atgaaggtat atcccaaagc ggaccgaaaa aatccctggg agcttatatg
      301 ggtgggcttc aacggagcgg atgcccggct gctcaccagc gcgatcggac tttcgccctc
      361 ctctccggtc gttcacacga atccgcttag ccgccagcga atcaaacagg ccttcgatgc
      421 agtctataac gcccgaggcg atcgccccag tcagatcgtg gcgatgaccg gtgcattgta
      481 caccctgctc tcgctcctga tggaactcag cggcacgcct tttccctaca cttcgggtca
      541 ggagtatatc gatccggcgt gcgatttcat tgcgctcaac ttcaaccgat caataaccgt
      601 tgaagatatc gcccggcatg tcggagtcag ccgctcctgt ctttaccggg cattcatcaa
      661 caacctctcc caatcacctc aggagtacct gacctcctac cggataaaat tttcatgcag
      721 ccttctggaa caaagcaatc tcccgcttaa ggagattgca tatcgttgtg ggttttccag
      781 ttcgctgtac tactcgaagg tgttcaaatc cgtgatgggc gtgccaccat ccgagtatca
      841 gatgcgctcc aatcccgaat gatctaacgg gcaagcagct tcgccgctga ttcctgatca
      901 aggaacaggt tccagtcggg gtgggttttc aggagcgtgg cggggatcat ccgatcgact
      961 ttggatgtga tcgtgttgta gaccgcctcc gctttcaccg cgtagggaac cgccgaaacg
     1021 atgtgcttgc aggacataat ctgctttgga ctcatcgtaa cggcggtctt gggaacatcg
     1081 ttcagcgtcg cgaaccatcc ttcgctcacc tgctgctgct tgcaccgctc gtccagttca
     1141 acgacgatat acgcgtcggt cgcctcgaag tccgccgggg gatcgttgaa ggcgatatgt
     1201 ccgttttcgc cgattccgac gattccgaca tcgacctgct tttccttaat gatggcggaa
     1261 agtttcgccc tcttcccttc aatgtcgccc tcgccgtcga cgaagtgggc ggctttcaga
     1321 ttgacctttg aaacgaaccg ctctttcaaa tacttgcgga agcttgcagg gtgggtttcc
     1381 ggcaggccca cgtattcatc aaggtggaac atctccacct tggaccaatc gacatcggat
     1441 tgaagcaacg cttccagcgt atcgaactgg ctggccccgg tggaaagaat gattctggct
     1501 tcacctttct ctttgatcgc cgcctgcaag agttcgacaa tcttcttggc tgcttttttt
     1561 cccaattgtt gcgggctatc ggcaatgaaa atcgaaatac tcatggtcac tcctccttat
     1621 ctgtcaatat gtgagtgttc gttccactca caaacacact ttgtattgta atatcttcat
     1681 tgaagataat caaatcggcc tgcttcccca cggcgatcga tcccgtcagg gaatcgaggc
     1741 ctacgaaccg cgccggtagc aggcttgcag ctttcaccgc tttcggcaga ttcaaaccga
     1801 cgtgttccat gagtgtccgg accatccggt ccgtggtgca cacactgccg gcgaatacgg
     1861 tcttgtcggg catcttggcg attccgtcct cgacgatcac gtcggttccg cctacaagcg
     1921 gaccgaggat cgacggtcct tccggcaacc cggcagcccg catgctgtcg gtggtggcac
     1981 agatgctgtc ggcatccttg cacttgtaga tcagctgaag cagctccggt ggcaggtgaa
     2041 gtccgtcggc gatgatctcc agcttcagac catcgatgag atacccgctt tcgatcactc
     2101 ccagtactct gaaaccgccg acccgggtga tcgttgacat ccccgaatag aagtgggtaa
     2161 gcagttcgac cccatggtcg accgccttca gcatctgatc gtaattggcg caggtatggg
     2221 cggcggaaag gtaaatccct tctttcttga gcacatccgc catctcatag gcgcccggca
     2281 gttcgggggc gaacgaccag cgccggatca acccttcgcc ggcttccaga atcggcatat
     2341 agtgtctagg gtccggcact cgaagatggc gctcctccat cgctcctttt tccttggggc
     2401 agaagtaggg tccctccagg tggagacccg gcaggtgcgg catccccttc gaggtggctt
     2461 ttgcagcttt aaaatccttg aatgagcgat agaggtgttc atcggtggaa gaaagggtgg
     2521 tcggcaaaat tgtggtcgca ccgaatttca tatgcgcctt ggcggcttga acgaacgctt
     2581 cgacgcttcc atccatataa tcgtagccac cggatccgtg ggtatggata tcaacgaaac
     2641 cggggctcag atagagacct ttgcagtcga tcaccttccc gtcgaacgaa ggtgccggca
     2701 tcggtccaaa cgccttgata acaccatctt cctcaagaag atacccatca tgaacaagcg
     2761 agtcctcaag gaccaaggtt gcattgataa acaggtgcac tcacccctcc tcactcctgt
     2821 gtacacggta ccatgtacct attccgccaa ccatattact ttgttgcagg agcatatcat
     2881 tatgttgcat ttatatatat ttggcttctc cgcgttccac ataccgatcc gtaacagcca
     2941 tgattaaccc gaccgcctca tcaagccctt ccttcgtgga tgaagcgacc tcccagcggg
     3001 caatcccctc ttcgcttcgg ctgattcgtt gggcgacttg caaccaagat ccctccaaat
     3061 cggaggactc atcgattttc aagggaaggc ctttgcgcat caaggtcgca tccagggaag
     3121 atccgtcttc catctccttg aagaccgtca gtccccggtc ggcaaagatg cgttcaacct
     3181 cgctgactac cggtttaagg tgcttgtcat caaagaaatg gcatggccat tctctccgac
     3241 ggtgaattga gtgctgcagc ttcatcgcct gttcatattt ttcgggatat tggctgtaca
     3301 tcctggctgt cagcagagca aagaacagcg tgttttccgt ggaggcgaac cctttggaaa
     3361 gatcgttgag gtcataggga aagttcatgt agtaatgggc ggattcttcg ctggcgagca
     3421 cgtattgatc atcaaggttc ttcctaagcg cttctttgat aaaagagtgc ccgtttcgaa
     3481 tgatgtgaac acccattccc gcttttgcct gaagtacgat ggaaagagga tttgctttca
     3541 catcgtaaaa caactgcggg ttaaagggag cggaacccca gtacccttga gcgtgaaccg
     3601 attcgaaata ggagcgaact tcggggatga tgatggacag attgaacgaa ggaggaacct
     3661 gcactccgtg ccggtccatc agatccatcc gatctccatc gccgtcgaac gcaaacccga
     3721 atgcccaatc tcccttcttc atcagctcgg tcgtcggtct cagcgactcc aaggctatcg
     3781 ggttgggctc cccggcgggg aaggtgccat cgggaatgag attccgaacg gtaagatccg
     3841 ccccgcttcg gatcagggcc tcggcaactt ccgttccggc agccccgctg aggaaatcga
     3901 ccatgatcgg cacccctttc aaggtggttt catgcacacc ggcaagggag cgggagtaat
     3961 cgatgaattg atcgaggtac cgcctgatat acacccgccc tcgtgttttt gtcgagggct
     4021 tattggaacc ggcagcgtag aaatcacgga ttgcggtgat gcctcctttc ggcccggctc
     4081 cgagcgccaa cgcttgcaac ggcggggcga ccagcttcaa tccgatgtat tcgccggggt
     4141 tgtgactcgc ggtgaacatg attcctccgg cggatggatg ctgcatgcaa ctgaagtaaa
     4201 attgacacgt cgagctttgg agggggttca cgatcacatc caacccgaac gtcgggaaaa
     4261 tctccagtgc ctcctccatc agtcgggggg ctgccaagcg ggcatcacgg ccaagaacga
     4321 tcgtatcaac cgaaagaatt tcgtgatagt atcgagcaat cgcctcaaga agacgaatgg
     4381 ataattcttt ggatacatgt tcgcttttgg tccggatgtc gtatgctttg aacatactcg
     4441 tattcagcgt aattcgctct gcttcagtca attgttggta gttcatcggt cttctcctca
     4501 tcttcggtca tatacattac tgttctcaaa atatgaaacg gatcttttac cggaagggcg
     4561 acaacccctt caaccggttg acccgatcgg tttcgaatct ggatccattc atttcccgtc
     4621 tcggcgggga atgtttcaaa ggtgtagctc actaaacggc gatgccacgc caaaaacact
     4681 tcatttcccg ttgcccggta gaagcgccat gtcgcataga ccgcctcggc gtggggccac
     4741 cagagcttgt aatcccaggt ggaggtgacc aagcgggaaa atagatccgc ttgagttttt
     4801 ccttgcggcg ggcttccgct gctgtcaata tatcgaaaca atccgccatg ttcttcgtcc
     4861 catcctcgtt caagcgaggc gagcacgacc tcacccatcc gaggaagatg gtcctccagc
     4921 acgttcagac agaaccacat cgactcgact gcatggccgg gattccggtg gcggctcagc
     4981 aatgactcct catttccatc ggtatgcacg atctcacgga ccaatgcggt ctttggatcg
     5041 acgaagcggt caagaatcgt cattgcatct tcttgtgcat cctcggcgac cgtttgggcc
     5101 tgatcattca acccgagttc ggccaacgcc ttggaaagct catcggcggt attgaccatg
     5161 atcattgaaa tcccgtgaga tcgggcggaa gagggaatcg gatacggctc gcttttgatc
     5221 atccctcgag cgatgaaccc tcgaatcttc gtatagagcg ccagggcttt gagaacgatc
     5281 gatcgactct ccacgaaacg cgcatagcgg ctgaagccga tgatgacaaa acaatcggca
     5341 tagatgcttg tgtacccctg tttgaccgta ccgtcccacc cgagcaggaa cgcgcatcct
     5401 tcatccgctt gatcgaggaa tgcatggcgg tcaagaaaca tgtgcaagag ccgcgcccgc
     5461 tccgtatacc ggcttcgttc ctcctcattc aatagacgtg caagagtcgg tgagccaagg
     5521 agatacgaga gaacccaaag cattcggcct tggctccacg tatacttgtc tcgggaaaca
     5581 agtgttttac cgtcattggt atagc
//
LOCUS       DAOPUZ010000015         5425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_15071_length_5425_cov_3.565177, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000015 DAOPUZ010000000
VERSION     DAOPUZ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15071_length_5425_cov_3.565177"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKN79_00600"
     CDS             complement(<1..403)
                     /locus_tag="PKN79_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ94299.1"
                     /translation="MKRLMLIATGLLLAAALIALPASEAAPKTLSYSATDASGRTITL
                     DGKPEKVLVAAKAALMPALALFLFPEAQEMEISLSKTDQGLGDFFPLVWPHINPGGRL
                     DQNASVEELITKQGDLILLKATHYESMGKKLD"
     gene            513..1229
                     /gene="yqeC"
                     /locus_tag="PKN79_00605"
     CDS             513..1229
                     /gene="yqeC"
                     /locus_tag="PKN79_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HNZ94300.1"
                     /translation="MTFSLFDELGAHIESLSARRIAVTGSGGKTSIIIGLADRFREEG
                     VLVTTTTHLASPSEMEYPFDEIAIDPNNPPKVEPGRQVLWAAPDSDKLGSVDPELLEK
                     TSRLAGYTLIEADGARRRPFKFHNEGEPVIPPWVDCVVAVVGLSGIGKRLDEETFHRE
                     REYRTATGSKEAWITLDTLDALLDHPDGILKGVSTHPVVLVYNQSDAACDEIVAEALR
                     LGRTKKRITAVLAGTCRGGV"
     gene            1226..2812
                     /gene="yqeB"
                     /locus_tag="PKN79_00610"
     CDS             1226..2812
                     /gene="yqeB"
                     /locus_tag="PKN79_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HNZ94301.1"
                     /translation="MNIFEEAAALQDQNIPFAFVSITKSVGSTPRSNAHMIVKKDGST
                     IGTVGGGIAEFTVTKEAVAAIAEGKSTHVDVSLAVADGHACGGTLEFFVDVIAGKRRL
                     LLFGGGHVNEQIARLGAGCGFRIEVIETRAEYATGERFPDAGEFHVGETVDEAMKSLV
                     IDRDCAIVIATHGLDKSVLEAVITSDAAYIGMLGSRTKVNTYRRALESEQNISIERLD
                     HFYSPVGLDIGSETPHEIAIAVMAEVMMVLHDRSGQSLSRRAENLVVVRGAGDLATGV
                     IVRLAKAGYRVCALEIEQPTTIRRTVAFSEAVYTGEVTLETVVCRRAESDQEAKTLLD
                     QGIVALMVDPSASMIERLRPFAVVDAIIAKKNLGTHKEMAPLVIALGPGFEAGADCDY
                     VIETKRGHDLGKVISRGFAEPNTGIPGKIGGFAEERVLHSASAGTFVGHKKIGDPVKQ
                     GDVIASVGTDEIIAPIDGVVRGMLHDGIVVPTNFKVADIDPRGIASYCETISDKARAL
                     GGSVLEVIDGMRAKAFRRIS"
     gene            2809..3849
                     /gene="selD"
                     /locus_tag="PKN79_00615"
     CDS             2809..3849
                     /gene="selD"
                     /locus_tag="PKN79_00615"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNZ94302.1"
                     /translation="MKKEVQLTAFSKFAGCGAKVTPAMLDKALCGLVQPYNPNVLSDF
                     NHAEDAGVYKFSDDLAIVQTVDFFPPVCDDPFFFGKIAATNAISDIYAMGGVPITAVS
                     IVSFPEEKLDSSYLTKILEGALDALKESGAALVGGHSIRDEQLMFGLCVVGTVHPKKM
                     WSNNRAQDGDLLLFTKPLGSGILQNAVKAKVAGKGDEERLLKVMSTLNKDAAEILKQF
                     DVHACTDVTGFGLLGHLSEMAIDNPNGFEIVMKDVPLMEGVRLYAEQGFIPGGSYTNR
                     EHRKHLISNLDELDETGQLLLFDPQTSGGLLAALTAREAHEALKVMREAGIEAAIIGR
                     VSKEIEGIVVRV"
     gene            3922..5250
                     /locus_tag="PKN79_00620"
     CDS             3922..5250
                     /locus_tag="PKN79_00620"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNZ94303.1"
                     /translation="MMSHSVKARIKALKESSRVLGVSTNEERIDLLKRVAAGLRRDWP
                     TIRKANEQDVAEAKEQKIGATLIKRLVFDEQKLNEVILGLKQVAALADPVGKVLQRRL
                     LDDGLILEQISVPIGVIGMIFESRPDALVQIVGLCIKSGNAIILKGGSEAKRTNLALV
                     ESIKQSSLGSSLGDGYVTLLESHGDVKEMLKMNDEIDLLIPRGSNAFVTYVMENTSIP
                     VLGHSDGICHLYIDEQADLKKALPIVIDAKTQYPAACNAIETLLLHASLVDVFLPEVG
                     KALKEANVTIHGDERTQAAIECIPLEEGDYFNEYLSLELNIRVVDSLEEAIEHIETYG
                     SHHTDAIVSENPEAVRRFFTEVDSADVFANCSTRFADGFRFGFGAEVGISTTKIHARG
                     PVGLDGLMSTKFLLAGSGQIVAAYSGKDAKRYLHKDLSVDAPSLVQGGSV"
     gene            5247..>5425
                     /locus_tag="PKN79_00625"
     CDS             5247..>5425
                     /locus_tag="PKN79_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNZ94304.1"
                     /translation="MKRDLPQVKRVVVKVGTNLLSTKAGIDEAYIDTIASQIAALMEK
                     GYQVLLVSSGAIGMGA"
BASE COUNT         1213 a         1425 c         1696 g         1091 t
ORIGIN      
        1 ggtcgagctt tttgcccatg ctttcatagt gggtcgcttt cagcagaatg agatcgcctt
       61 gcttcgtaat cagctcctcg acgcttgcgt tctggtcgag ccttccgccg ggattgatgt
      121 gcggccagac gaggggaaag aaatccccga gcccctggtc ggtcttggaa aggctgatct
      181 ccatctcctg cgcttccggg aacaaaaaga gcgcaagagc cggcatgagc gcggctttgg
      241 cggcgacaag aaccttttcc ggtttgccgt cgagggtgat ggttctcccg cttgcatcgg
      301 tggcgctgta ggaaagagtc ttcggtgccg cttcgcttgc aggcagcgca atgagggctg
      361 cggccagcaa caagccggtt gcgataagca taagacgttt cattgattcc tccttggtgt
      421 gctgtaagcg taccattatg tatttaaaaa tacaatcgaa tttttccatt ttataattga
      481 ctgtataatg gcaggcgatt agacttgggt gtatgacttt ttccctcttt gatgaactcg
      541 gagctcacat cgaatcgctt tccgccagac ggatcgccgt taccggaagc ggcggaaaga
      601 caagcatcat catcggtttg gcggatcggt tcagggagga aggagtgctt gtcacgacga
      661 ccacccatct ggcttcgccg agcgagatgg agtacccgtt cgatgagatc gcaatcgatc
      721 cgaacaatcc gccgaaggtg gagcccggtc gtcaggtgct ctgggcagca cccgattcgg
      781 acaagctcgg gagcgtggat cccgagctgc tcgaaaagac gagcaggctg gcgggataca
      841 cccttattga agcggacggg gcgcggagaa gaccgttcaa atttcacaat gagggagaac
      901 cggtgattcc tccgtgggtc gattgcgttg tcgccgtcgt cggactgagc ggaatcggga
      961 agcggttgga cgaggagacg ttccatcggg agcgggagta tcgaaccgcc accggatcga
     1021 aggaagcgtg gattaccctc gatacgcttg atgccctgct tgaccatccc gacgggattt
     1081 tgaaaggagt gtcaacccat ccggtggttc tggtctataa tcaaagcgat gccgcatgtg
     1141 acgagatcgt cgccgaagcc cttcgtctgg gacgaaccaa aaaacgaatt acagcagtcc
     1201 ttgccgggac ctgtcgaggt ggagtatgaa catatttgaa gaggcagctg ctctgcagga
     1261 tcagaatatc ccgttcgcct tcgtgtcgat caccaaaagc gtcggctcca cgccgcggag
     1321 caacgcccac atgatcgtca aaaaagatgg gagcacgatc ggaaccgtcg gcggggggat
     1381 cgccgagttc acggtgacca aggaagcggt cgcagcgatc gctgaaggaa aaagcaccca
     1441 tgtggacgtc tcgctggccg ttgccgacgg tcatgcctgc ggtggaacgc ttgagttttt
     1501 cgtcgacgtg atcgccggca agcgtcgcct gctgttgttt ggcggggggc atgtgaatga
     1561 acagatcgcc cgcctcggag cgggatgcgg gttccggatc gaagtgatcg aaacccgggc
     1621 ggagtatgcg accggggaac gctttcccga tgccggggag ttccatgtgg gggagacggt
     1681 cgatgaggcg atgaagagcc ttgtgatcga tcgggattgc gccatcgtca tagcgaccca
     1741 cggcctggat aaatccgtgt tggaagcggt catcaccagc gacgccgcct acatcggaat
     1801 gctgggttcc cgcaccaaag tgaacaccta tcgaagagct ttggagagtg aacaaaacat
     1861 ttccatcgaa cgcttggacc atttctacag cccggtcggt cttgacatcg gatccgagac
     1921 ccctcacgag atcgcgatcg ccgtgatggc cgaggtgatg atggtgctcc atgaccggtc
     1981 cggtcagagt ctcagcagaa gggcggaaaa cctcgtcgtg gttcgcgggg cgggggatct
     2041 ggccaccggc gtgatcgtcc ggcttgcaaa agcgggatac cgggtatgcg cccttgaaat
     2101 cgagcagccg acgacgatcc gccgaaccgt agccttcagc gaagcggtgt acaccgggga
     2161 agtgacgctt gaaacggtag tctgccggag ggccgagagc gaccaggagg cgaagacgct
     2221 gctcgatcag gggattgtcg cgctgatggt cgatccgagc gcttcgatga tcgaacgtct
     2281 tcgacccttc gcggtcgtcg atgcgatcat cgccaagaag aacctgggaa ctcacaaaga
     2341 gatggcgccg ctggtcatcg ccctcggacc cggctttgaa gcgggggccg attgcgatta
     2401 cgtgatcgaa acgaagcggg ggcatgacct cgggaaggtc atcagtcggg ggttcgccga
     2461 accgaatacc ggtattccgg ggaagatcgg cggctttgct gaggaacgag ttctgcattc
     2521 tgcaagcgca ggcacgtttg tcggccataa gaagatcggt gacccggtca aacagggcga
     2581 cgtgatcgcg tcggtcggaa cggatgagat catcgctccg attgacgggg tggtccgggg
     2641 gatgctgcac gacggaatcg tcgttccaac gaacttcaag gtggcggata tagacccgcg
     2701 cggcatcgca tcatactgcg agacgatcag tgacaaggcc cgggccctcg gtggatcggt
     2761 attggaggtc atcgacggga tgcgggcaaa agcatttcgg aggatttcat gaaaaaagaa
     2821 gtacaattga cagcattttc caagttcgcc ggctgtgggg cgaaagtgac ccccgccatg
     2881 cttgacaaag cgctctgcgg gcttgtccag ccgtataatc ccaatgttct cagcgatttc
     2941 aatcacgccg aggatgcggg ggtgtataag ttcagtgacg atcttgcgat cgtccagacg
     3001 gtggatttct ttccgccggt gtgtgacgat cccttcttct tcggcaagat cgccgccacc
     3061 aacgccatca gcgacatcta tgcgatgggc ggcgtgccga tcacggcggt gagcatcgtc
     3121 agctttcccg aagagaaact tgacagctct taccttacta aaatccttga aggcgctctt
     3181 gatgccttga aggaaagcgg ggccgccctt gtcggcggac acagcatcag agatgaacag
     3241 ctgatgttcg gtctgtgcgt cgtcggaacc gttcatccta agaagatgtg gtcgaacaac
     3301 cgggctcagg atggagatct tcttctgttt acaaaaccgc tgggttcggg aatcctgcag
     3361 aacgcggtga aagcgaaggt cgccggaaag ggggatgagg aacgcctctt gaaggtgatg
     3421 agcaccctca acaaggatgc ggccgagatt ctcaagcagt tcgatgtcca tgcatgcacc
     3481 gacgtcaccg gcttcggcct gctcggacac ctcagcgaga tggcgatcga caaccccaac
     3541 gggttcgaaa tcgtcatgaa ggacgtcccg ttgatggagg gagtccggct ctatgccgaa
     3601 cagggcttca tccccggagg cagctatacg aaccgggagc atcggaagca cctgatctcc
     3661 aacctggatg agcttgatga aaccgggcag ctgcttctct tcgatccgca gacctcggga
     3721 ggcttgctcg ccgccctgac cgcccgggag gcgcatgaag cgttgaaggt catgcgcgag
     3781 gccggcatcg aagcggcgat catcgggcgg gtctccaagg agatcgaggg aatcgtcgtc
     3841 cgggtttaaa aggggaaaac aacgcaggct gccttgatac cgatggctcc ttccgggtaa
     3901 tctagcgtaa ggagcacgag tatgatgagt cacagcgtga aggcgcggat caaggcgctg
     3961 aaagaaagtt cccgagtcct cggcgtgagc acaaacgaag agcggatcga tctgctgaag
     4021 cgggtagccg cagggttgag gcgggattgg ccgacgatcc gaaaagccaa cgagcaggat
     4081 gtcgctgaag cgaaggagca aaagatcggc gccaccttaa tcaagcgcct ggtctttgac
     4141 gaacagaagc tcaatgaggt gatcctgggc ttgaagcagg tagccgcttt ggccgacccg
     4201 gtcggcaagg ttctccaacg gcgtctgctg gatgacggac tgattctgga gcagatctcc
     4261 gttccgatcg gagtgatcgg aatgatcttc gagtcgcggc ccgatgcgct ggtccagatc
     4321 gtcggactgt gcatcaaaag cggaaacgcg atcatcctca aaggcggcag cgaagcgaaa
     4381 cggaccaacc tcgctctggt ggagtcgatc aaacaaagca gcctgggatc ctccctcggg
     4441 gatggctatg tcaccctgct tgaaagccac ggcgacgtta aagagatgct gaagatgaac
     4501 gatgagatcg atctgctcat cccccgcgga tccaatgcgt tcgtcaccta cgtgatggaa
     4561 aacacctcga ttcccgtcct cggtcacagc gacggaatct gccacctcta catcgacgag
     4621 caggcggatt tgaagaaggc gcttcccatc gtcatcgatg ccaagacgca gtatccggcg
     4681 gcgtgcaacg cgattgaaac cctgctgctt catgcttcgc ttgtcgatgt gttcctgccg
     4741 gaggtcggca aggcgctcaa ggaagcaaac gtgacgattc acggtgatga gcggacgcaa
     4801 gcggcgattg agtgcatccc gcttgaagaa ggtgattatt tcaacgaata cctttcattg
     4861 gaactgaata tccgggttgt cgattccttg gaagaggcga tcgagcatat cgaaacatat
     4921 gggtcccacc acaccgatgc gattgtcagc gagaatcccg aggcggtcag gagatttttc
     4981 accgaagtcg attcggcgga cgtgtttgcg aactgttcga cccgcttcgc cgacggattc
     5041 cggttcggct tcggggcgga ggtgggaatc tccacaacga agatccacgc ccgcggtccg
     5101 gtcggcctgg atgggctgat gagcacgaag ttcctccttg ccggttcggg tcagatcgtg
     5161 gctgcctaca gcggcaagga tgcaaaacga tacctccaca aggatctttc cgtcgatgct
     5221 ccttccctgg tgcaaggagg atcggtgtga agcgcgacct tcctcaagtc aaacgagtcg
     5281 tagtaaaagt cggcacgaac cttctctcca cgaaagcggg gatcgatgag gcgtatatcg
     5341 atacgatcgc aagtcagatc gccgcgttga tggagaaggg atatcaggtg ctgctcgtct
     5401 cctccggtgc gatcggaatg ggggc
//
LOCUS       DAOPUZ010000016         5424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_15076_length_5424_cov_3.668840, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000016 DAOPUZ010000000
VERSION     DAOPUZ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15076_length_5424_cov_3.668840"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(410..970)
                     /locus_tag="PKN79_00630"
     CDS             complement(410..970)
                     /locus_tag="PKN79_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94305.1"
                     /translation="MNKRMIVFVCLLVFAVGGVMAYENMTTVGLDLGALAYPDKDPVT
                     FSGIHTGFYSYPDEFSASGFYAQVSAQTMVTENRSDWFSTYLMNVLLGYSHRIELGRM
                     FDLVVGVGPSYQMLHVEVLGGILNSSASMIGFGTSVEANFLLSPSLTINAGLSVLAGI
                     YDLHDKKVLEEIPATARTYIGVGFGF"
     gene            complement(1104..1676)
                     /locus_tag="PKN79_00635"
     CDS             complement(1104..1676)
                     /locus_tag="PKN79_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94306.1"
                     /translation="MNKRMIVFVCLLVFAVGGVMAYSSMSTVGLDYGAVGTLRDSGKK
                     PLSFTGIQLGVYTYSDEYGANGFYSQVSFQLSNTEGDPDDLFFAYMMNIVSGYTRRFD
                     LGGMFDLLIGVGPSYQQFDLNILGGAITGKGSMIGLAATAEANFLLLPWMTINWGATA
                     HVGIYDLTDRKVIDKKPITARTYVGAGFTF"
     gene            complement(1852..2421)
                     /locus_tag="PKN79_00640"
     CDS             complement(1852..2421)
                     /locus_tag="PKN79_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94307.1"
                     /translation="MHKKIIVFVILVVFALGSVMAYSSMTTVSLDYGAVGTIQDFDKE
                     PTNFIGFQFGVYTYSDECSPDGFYSQVSFQTLVTDDSNDLFTKDLVNIVFGYARRIDL
                     GGMFDLMVGGGPSYQKSELENAGGQIIDKASMIGLAATAEANFLLSPRMTINWGVTTH
                     VGIYDVKGRKLITKNPVTARTYMGVGFTF"
     gene            2810..3022
                     /locus_tag="PKN79_00645"
     CDS             2810..3022
                     /locus_tag="PKN79_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HNZ94308.1"
                     /translation="MDELAVGQKGSVKAINAPKQLKRRLMDMGFTKGVGVEVVKMAPM
                     GDPMEISVRGYHLCLRKAEARSIELV"
     gene            3026..4990
                     /gene="feoB"
                     /locus_tag="PKN79_00650"
     CDS             3026..4990
                     /gene="feoB"
                     /locus_tag="PKN79_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNZ94309.1"
                     /translation="MHTIALVGNPNSGKTTLFNALTGSTAYVGNWPGVTVEKKEGLSN
                     GHKILDLPGIYSLSPYSPEEQISRRYILNEQPDLIIDVIDATNLERSLYLSTQLAELG
                     RPLLLALNMEDLLVKDGIAIDTKRLSQMFGTPVVQISATKGTGIAELKEEIEHALSNP
                     QTPPCPLFSSSIERKLTTLIEDDYLHEIPHGRQMRWAAIKLLEGDELFLETMPPIPPE
                     LNAYLEKTRSSLEGLYDDDVESIIVDQRYKVAERLARESLIQKEAKEKRFTFDTIATH
                     RIWAIPLFILIMSGVFFLSVGVVGGGTTALLEGFFENLAEWVVATGESWQVHPFLIGI
                     LSDGIIAGVGAVLTFVPQLFILFLLLSILEDCGYMARIAFIMDRQMRSMGLSGKSIIP
                     LVIGTGCTVPAIMSTRTIEHQKQRELTIIVTPFIPCGAKMPIFALMLTYFFRNKWYMA
                     PLMYVMGILAVIVTGLLARAIDRHKETNAFILELPRYQIPSPTSVWRQTKDRTLGFIY
                     KAGTIILLASILIYLLSSYSFAFKAVDAEESMLALLGRVIAPVFIPLGFGFWQAAVAL
                     LTGIAAKETIVSTLSVVIGTSSLTSFFDPAGALAFMTFILLSSPCVAAMAAMLKELGS
                     KRKFLFAILWQFGFAYLVSLLVRYIVLLII"
     gene            5000..5146
                     /locus_tag="PKN79_00655"
     CDS             5000..5146
                     /locus_tag="PKN79_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HNZ94310.1"
                     /translation="MKTIIANTIVAIFILALVVFALSRIVPAKKDDDSSSKCRNCPFS
                     GQCH"
BASE COUNT         1370 a         1486 c         1314 g         1254 t
ORIGIN      
        1 cattcgggta tgaataaaac ccaacctgta ttcctgtgaa gttgagcaaa tcctccaaat
       61 cttttgtgga ggacagaccg ccataatcca agctcacagt gaccatgttg tcgtaagcca
      121 tcacgccgct gattgcaaaa acaagcacac agatgaatac aaccaccctt ttattcatga
      181 gactcctcca agaaaaccaa tgtccctagt atacgggcaa acgattcgtt tgtggtaata
      241 aatttacgga ttcgataaaa ataaatacaa tataaatccg gaagttgaaa attcacacca
      301 agagaagcgc ccggtgctca tacacggtaa ggccataacg atcggtatta caaaacgtta
      361 tggccggcat atcttccttt acggtgcgga agacgggctg gaagaaggat cagaagccga
      421 aaccgacacc gatgtaggtt cgggcggtgg cgggaatctc ctctaggact ttcttgtcgt
      481 gcaggtcata gattccggca aggacgctca gtcccgcatt gatcgtcaaa ctcggcgaga
      541 ggaggaagtt cgcctcgacg cttgtaccga atccgatcat cgatgcacta ctgttgagga
      601 ttccccccaa cacctcgaca tgaagcatct gataggaagg accgacgccg acgacgagat
      661 cgaacatcct gcccagttca atccggtggc tgtaccccag aagaacgttc atcagatacg
      721 tggagaacca atcgctccgg ttttccgtaa ccatcgtctg ggcggacacc tgagcgtaaa
      781 aaccgcttgc gctgaattca tcgggatatg aatagaaccc ggtatgtatt cccgagaatg
      841 tgaccggatc cttgtcgggg tacgccagcg cccccagatc caagcccacg gtggtcatgt
      901 tctcatacgc catgacgcca ccaaccgcaa acactaagag acaaacgaac acgatcatcc
      961 gcttattcat ggaacacctc cagaagattt catgcccata gtataccgat aaacgatcct
     1021 tttggggcaa atattattca cgaagaaaat cattttgcgc aggtggaagt tacaactcag
     1081 gccatgacga cacggaagac cgatcagaag gtgaaaccgg ctccgacgta tgtccgggcg
     1141 gtgatcggct tcttgtcgat gactttccgg tctgtcaggt catagatccc gacatgggcg
     1201 gtcgctcccc agttgatcgt catccacggt aaaaggagga agttcgcctc ggcggtcgcc
     1261 gccaacccga tcatcgaccc tttcccggtg atcgctcccc cgaggatatt caggtcgaac
     1321 tgctggtagg aaggtccgac cccgatcagc aggtcgaaca tgccgccaag gtcgaaccgg
     1381 cgggtatacc cggaaacgat attcatcata tacgcgaaga acagatcgtc cggatcaccc
     1441 tcggtgttcg acaactggaa ggagacttgg gaataaaacc cgttcgcgcc gtattcatcc
     1501 gagtaggtat agaccccgag ctggattccc gtgaagctga gcggcttctt tccggaatcc
     1561 cggagggtac ccaccgcacc gtagtccaga cccaccgtgg acatgctgct gtatgccatc
     1621 acgccaccaa ccgcaaacac taagagacaa acgaacacga tcatccgctt gttcattgag
     1681 gacctccgga aagatttcat gcccttagga taccgataaa cgatccattt gaggccaata
     1741 ttgatcacga agagaatcat tctgcgcagg tggaggttac aaatcaggcc atgacgacat
     1801 cgtcacggcc ctgttcatac cgttcgggaa ccgggttggt cggaagagcg atcagaaggt
     1861 gaatccgaca cccatgtagg ttcgggcggt gaccggattc ttggtaatca gtttccggcc
     1921 ttttacgtca tagattccga cgtgggtggt caccccccag ttgattgtca tccgcggtga
     1981 aagaaggaag ttcgcctcgg cggtcgccgc cagtccaatc atcgatgctt tgtcgataat
     2041 ctggcctccc gcattctcca gctcggactt ctggtaggag ggcccgcctc cgaccatcag
     2101 gtcgaacatg ccgccgaggt caatccggcg agcatatcca aatacaatgt tcaccagatc
     2161 cttggtgaac aggtcgttcg aatcgtccgt gaccagcgtc tggaacgaga cctgggaata
     2221 aaacccgtcc ggactgcatt catccgagta ggtatagacc ccgaactgga atcctatgaa
     2281 gttagtcggc tccttgtcaa aatcctggat ggttcctacc gcaccgtagt ccagactcac
     2341 cgtggtcatg ctgctgtagg ccataacgct gccgagtgca aacaccacca gaataacaaa
     2401 cacaattatc tttttatgca tgaaacgcct ccaaaacata tggcctcagt atagagataa
     2461 acgatacggc gtatcaatta aaccaactgc cgaattgatc atttcaaccc caaaacaaca
     2521 agaacatatg acatatatat ttaaagatta ttaatttata aattcttata aataaataag
     2581 taatataact aatttacaaa tattatacat atttttaaca ttaatatcat atactattga
     2641 catatatttt ggatgccgat ccggatctca aacctaagaa gacagtcgcc ctcttttgga
     2701 aggcaaccga tgaaaccatg catatctgta gcccgaaagc agatatatct tgacagcatt
     2761 gtttgtttta actaacttat agctgaggaa tacaccgatg cggaacacga tggatgaatt
     2821 ggctgttggt caaaaaggtt cagtgaaggc gatcaatgcg cccaaacagt tgaaacgacg
     2881 attgatggac atgggattca ccaaaggtgt cggtgtcgag gtggtcaaga tggctccaat
     2941 gggtgatccg atggagattt ccgtccgagg ctatcatctc tgtctgagaa aagcggaagc
     3001 acgctcgatc gagctggtct gatctatgca cacaatcgct ctcgtaggga atcccaacag
     3061 cgggaagacc actttgttca acgcgttaac cggttcgacc gcctatgtcg ggaactggcc
     3121 gggggttacc gttgaaaaga aagaagggct gagcaatgga cacaagatcc tcgatcttcc
     3181 cggcatctat tcgctttccc cctactctcc cgaagagcaa atctcccgcc gatacattct
     3241 caatgaacaa cccgatctca tcattgatgt cattgacgcc accaatctgg agcgaagcct
     3301 ctacctctcg acccagctcg ccgagcttgg tcgtccctta ttgttggctc tcaacatgga
     3361 agacctgctc gtcaaggacg gaatcgccat cgacacgaag cgcctcagtc aaatgttcgg
     3421 aaccccggtg gtccagattt cagccaccaa gggaacgggc atcgccgagt tgaaagagga
     3481 gattgagcac gctctttcca atccgcagac gcccccctgc ccgctgttca gctcctcgat
     3541 cgaacggaag ctcaccactc tcattgaaga cgactatctt catgagattc cccatggaag
     3601 acagatgcgt tgggcggcga tcaagctctt ggaaggcgat gaactgtttt tggagacgat
     3661 gcccccgatt ccacccgaac tgaatgccta tcttgaaaag acccgctctt ctctggaagg
     3721 gctctatgat gacgatgtcg aatcgatcat cgtcgaccag cgctataaag tggcggaaag
     3781 acttgcaagg gaaagtctca tacaaaaaga ggccaaagag aagcgcttca ccttcgatac
     3841 gatcgcgacc caccgaatct gggcgattcc cctcttcatc ctcatcatga gcggagtgtt
     3901 cttcctttcc gtcggagtcg tcggcggtgg aaccaccgcc ctcctggaag gattcttcga
     3961 aaacctcgcc gagtgggtag tcgctacagg tgaatcttgg caggttcatc cgttcctcat
     4021 cgggattctt tccgacggga tcatcgccgg agtcggagct gttctcacct tcgtccccca
     4081 gctgttcatc ctctttctct tgctctcgat tcttgaagat tgcggctaca tggcccggat
     4141 tgcgttcatc atggaccgcc agatgcgctc aatgggtctt tcaggaaaat cgatcattcc
     4201 gcttgtcatc ggaaccggct gcaccgtccc tgcgatcatg agcacccgga cgattgagca
     4261 ccagaagcag cgggagctta cgataatcgt gactccgttc atcccctgtg gagcgaagat
     4321 gccgatcttc gccttgatgc tcacctactt cttccggaac aagtggtata tggctccctt
     4381 gatgtacgtc atgggaatcc tggccgtgat cgtcaccggc ctgcttgccc gggcgatcga
     4441 ccgccataag gaaaccaatg cgtttatcct tgaactgccc cgctatcaga ttccctcccc
     4501 tacgagcgtc tggcggcaga cgaaagaccg gacgctgggc ttcatctaca aagccggcac
     4561 gatcatcctg ctcgcttcga tcctcatcta cctgctttcc tcctactcct tcgccttcaa
     4621 ggcggtcgat gcggaagaaa gcatgcttgc gcttctggga agggtcatcg ccccggtctt
     4681 cattccgctg gggttcgggt tctggcaggc ggcggtggcg ctgctcaccg gcattgcggc
     4741 aaaagaaacg atcgtctcga cgctcagcgt cgtgatcggc acctcctcgc tcacgagctt
     4801 cttcgatccc gccggagccc ttgcgttcat gaccttcatc ctgctttcct ccccctgcgt
     4861 cgcggcgatg gccgcgatgc tcaaggagtt gggcagcaaa cggaaattcc tcttcgccat
     4921 cctctggcag ttcggattcg cctatctcgt gtccctcttg gtgaggtaca tcgtccttct
     4981 catcatataa ggtggcgata tgaaaacgat cattgcaaat accatcgtcg cgatcttcat
     5041 cctcgcactc gtcgtgttcg ccctgagccg gatcgtcccg gcgaagaagg acgacgactc
     5101 ttcatccaag tgcaggaact gccccttcag cggtcaatgc cattgaagcg aggatcgggt
     5161 gaagaaatac tatgcacaga tttgcataaa tatgcaaaaa cactggatat tatcttcaag
     5221 attcgctaca ctcttcctat catttgtccg caaggagtgg ttctatgaaa cgtattatac
     5281 tggtcggtct catcgtcatc cttggcgcaa gtcttttcgc cgcaggaagc aaggaacaaa
     5341 gtgatgttga taattccctt gacaaagtga tgaagaaagg gaagttcatc atgggtctgg
     5401 atgacagctt ccctccgatg ggtt
//
LOCUS       DAOPUZ010000017         5231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_15850_length_5231_cov_3.395286, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000017 DAOPUZ010000000
VERSION     DAOPUZ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15850_length_5231_cov_3.395286"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /gene="glpK"
                     /locus_tag="PKN79_00660"
     CDS             <1..1252
                     /gene="glpK"
                     /locus_tag="PKN79_00660"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNZ94311.1"
                     /translation="ETIVAWEKESGKVYHNAIVWQDLRTSAYIEELKDSPQGPIIEEK
                     TGLVLSPYFGGSKIAWLLEHVDGLREDAESGKAVFGTIDSWLAYQFSGKILHITDASN
                     ASRYLLMNIETLSWDDELLEIFSIPKGSLPMIVPSSGMVYGHTAKDGPFGSEVPIAGI
                     LGDQQAALFGQACFKPGMGKSTYGTGCFLLVNTGTDLARSQNGLLTTVAYKIGDEKPT
                     YALEGSVAVAGSLVQWARDNLKLVESAQQLDREALTVTDSGGVYVVPAFSGLFAPYWR
                     SDARGVIAGLTAFVTKAHLCRAILEATAFQAYDIFKAMESDSGIRTTELKVDGGMTNS
                     EPLMEFQADLLGVPVIRPQVVETTALGAAYAAGLSVGVYKDLDELSSYWKERKRWEST
                     MDDETRNEKVRFWKKAVERTLEWV"
     gene            1269..2090
                     /gene="cdaA"
                     /locus_tag="PKN79_00665"
     CDS             1269..2090
                     /gene="cdaA"
                     /locus_tag="PKN79_00665"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNZ94312.1"
                     /translation="MFTDIIQGILAFLRPAVQIGILAWVFYRFYAAIAQTKALALVRI
                     IIVLFAVYALSFLFKLDVLLWFYRYITVPTTILLCVVYQAELRRAFSQLWSGGGRIFR
                     LGTQTTSSDQIDSILNACNVLVNKQRGALIVFPRKLPIRQIIDSGTRLNADLSSSLIL
                     TVFDHDTPLHDGAMVIQGGKILAAGCYLPLSEQTDIKKSFGTCHRAALGLAEESDAVV
                     LIVSEETGAISLTYNANLYYDLSIPTVKRMLLALFSYHDITPEEVMKEAGSEEVE"
     gene            2074..2481
                     /locus_tag="PKN79_00670"
     CDS             2074..2481
                     /locus_tag="PKN79_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNZ94313.1"
                     /translation="MKRLNKYLQGFLVNWPAKVLSLLLAVAVYAFIHYSTIGARSVTI
                     PITVLLPERMEAQSLVPDSIDVEIRGDEHIIYLINPDSITAVVDFSKVEKEGITTANV
                     ILTHDREVFERSEITLKASPSSVRVLFGPRSDP"
     gene            2478..2840
                     /gene="acpS"
                     /locus_tag="PKN79_00675"
     CDS             2478..2840
                     /gene="acpS"
                     /locus_tag="PKN79_00675"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNZ94314.1"
                     /translation="MIGIDVVAIRRIEDLSEHARKRIFHPEELAQAERSPRPGEFLAG
                     RFAVKEAFFKAVGNGMKTLSTQDVWTTRGEHGEPVINLSSKARALTGERTVTLSISHD
                     DPVAVAVVLISGGNDGAL"
     gene            2827..3675
                     /gene="truA"
                     /locus_tag="PKN79_00680"
     CDS             2827..3675
                     /gene="truA"
                     /locus_tag="PKN79_00680"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNZ94315.1"
                     /translation="MARSDWKRPALVPIEAGKRRIKMILSYDGTPYSGWAYQTNAPTV
                     VGVLQQTIREMIDEEVEVIGSGRTDSGVHAAAQVCHFDIAHRNIKSGKFKPALNRLLP
                     QSIRILHSEEVDGSFHARYTTMGRTYRYYFKREGELLGFDEHRVAKLKAFPPLPLLDG
                     YAKILVGTHDFTTFAAASDKSESKVRDIYESYWKIEKDRFGAELLTYTISGNAFLHKM
                     IRSLVGSMIQFAGQKMGVEEFVEILEAKDRFRAGRTAQACGLYLYRITYDEEEYAWFE
                     EEYGGH"
     gene            3665..4390
                     /locus_tag="PKN79_00685"
     CDS             3665..4390
                     /locus_tag="PKN79_00685"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNZ94316.1"
                     /translation="MDIEDSIRTFVTICDEAEALISDREVEPTSTGDLSPVLDLFITP
                     SFDKNRIDQMSFKHLEEAVRGCSLCRLAETRTKTVFGTGVPSPTLMVIGEGPGGDEDL
                     QGEAFVGRAGKYLDAWLNAINLSRTNGVYITNIVKCRPPNNRDPQGDEVEACVPYLSR
                     QIQLLQPQTILLLGRVAAQTLLGRKDALAALRGTFHRYEGIPVLVTYHPAAVLRDQRL
                     KRPVWEDLKKIASFLNIALPGRS"
     gene            4390..>5231
                     /locus_tag="PKN79_00690"
     CDS             4390..>5231
                     /locus_tag="PKN79_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ94317.1"
                     /translation="MALYADVLVDVPLKQSFTYLVPEELQEASLFGCRAVVPFGSREV
                     TGYIVGVRDTDDSPFELKELKRMIDSEPVFTDKTVELVRWISRFYLCSEGEAISLVIP
                     GGRRDSAYGGLEQDEAFSAGRIERLSAEQEAALSSILNSDERMFYLHGVTGSGKSEVY
                     LRTAEAVIAEGKGVIYLVPEITLTHQLAVLVAGRFSNRVAILHSALTPSQRLKQWRRI
                     LNGEVDLVIGARSAVFAPVENLGLIIIDEEHENSYKSGSTPRYHARQVAQHRPVSQRG
                     QLRA"
BASE COUNT         1116 a         1503 c         1560 g         1052 t
ORIGIN      
        1 cgagacgatc gtggcgtggg agaaggagag tggaaaggtc tatcataatg cgatcgtctg
       61 gcaagatctt cgaaccagcg catatatcga ggagctgaag gacagtccgc aaggcccgat
      121 catcgaagag aaaaccggac tggtcctcag tccctatttc ggcggatcga agatcgcctg
      181 gcttttagaa catgtcgacg gattgcggga ggacgccgaa tcgggcaaag cggtcttcgg
      241 aacgatcgat tcctggcttg cctaccagtt ttccggcaaa atcctccaca tcaccgatgc
      301 cagcaacgct tcccgctatc tgctgatgaa catagaaacc ctttcctggg atgatgaact
      361 gcttgaaatc ttttccatcc cgaaaggttc gttgccgatg atcgtgcctt ccagcggaat
      421 ggtgtatggc cataccgcca aagacggtcc gttcggatcg gaagttccga tcgccggaat
      481 tctcggtgat cagcaagcag ccctcttcgg ccaagcgtgc ttcaagcccg ggatgggcaa
      541 gtcgacctac ggcacgggct gcttcctgct ggtgaataca gggaccgatc tcgcccggtc
      601 gcaaaacggc ctgcttacca ccgtcgcgta caagatcggt gatgaaaagc cgacatacgc
      661 gcttgaaggg tcggtggccg tggccggttc gcttgtccag tgggcgcgcg acaacctgaa
      721 actggttgaa agcgctcagc agctggaccg cgaagcgctt acggtaacgg actcgggcgg
      781 agtctatgtg gttcccgcgt tcagcgggtt gttcgccccc tattggcgga gcgacgctcg
      841 gggagtgatc gccggcttga cggcgttcgt caccaaggcc cacctctgcc gggcgatcct
      901 ggaggccacg gcgttccaag cgtatgatat cttcaaagcg atggagagcg acagcggcat
      961 cagaacgacg gagctgaagg tcgacggagg aatgacgaac agcgagccgc tgatggaatt
     1021 ccaagcggat ctgctcggcg tgccggtcat ccggccccaa gtcgtcgaga ccaccgcctt
     1081 gggggctgca tatgccgcag gactctccgt cggagtatac aaggacctcg atgagctttc
     1141 ctcctactgg aaagaaagga agcgatggga atcgacgatg gatgatgaaa cccggaatga
     1201 aaaagtccga ttttggaaaa aagcggtcga gcgaacactt gagtgggtgt aggaaaggag
     1261 cggtctagat gttcaccgat atcattcagg gcatcctcgc attcctccgg cctgcagtgc
     1321 agatcggaat ccttgcatgg gtgttctatc gcttctacgc ggcgatcgct caaaccaagg
     1381 cgcttgcgct cgtccggatc ataatcgtcc tcttcgccgt ctacgcgctg agcttcctct
     1441 tcaagctgga tgtgcttctg tggttctatc ggtacatcac ggttccgacg acgattctgc
     1501 tttgcgtggt ctatcaggcg gagctgaggc gggcattcag ccagctgtgg agcggcggtg
     1561 gccggatttt ccggcttgga acccagacga cgagcagcga ccagatcgat tcgatcctca
     1621 atgcatgcaa cgtgctggtg aacaaacagc ggggggcatt gatcgtcttt ccccgaaagc
     1681 ttccgatccg ccagatcatc gacagcggga cccgcctcaa tgcggatctt tcctcctccc
     1741 tgatcctcac cgtgttcgat catgacacgc cgctccatga cggggcgatg gtgattcagg
     1801 gcggcaagat tctcgccgcc ggctgctact tgccgttgag tgagcaaacg gatatcaaga
     1861 aatccttcgg gacctgccac cgggcggccc tcggccttgc cgaggaaagc gatgcggtcg
     1921 tgctgatcgt cagcgaagag accggggcga tcagcctgac ctacaacgcg aacctctact
     1981 atgacctttc gattccgacg gtcaagcgga tgctgctggc gctcttcagt tatcatgaca
     2041 tcaccccgga agaggtgatg aaggaggcgg gaagtgaaga ggttgaataa gtaccttcag
     2101 ggattcctgg tgaactggcc cgcaaaagtc ctttcattgc tccttgcggt agcggtctat
     2161 gcgttcatcc attactcgac gatcggggcc cgcagcgtta cgattcccat caccgtgctg
     2221 ctccccgaac ggatggaggc acaatccctt gtgcctgatt cgatcgatgt ggaaatccgc
     2281 ggcgatgaac acatcatcta cctgatcaat ccggattcga tcaccgccgt cgtcgatttc
     2341 tccaaggtgg agaaggaggg gatcacaacg gcgaacgtca ttctcaccca tgaccgggag
     2401 gtgtttgagc ggagcgagat caccctcaag gcttcgccaa gctccgttcg ggtgctcttc
     2461 ggtccaagga gcgacccttg atcggaatcg atgtcgtggc gatcaggcgg atcgaggatt
     2521 tgagtgagca tgccagaaag cggatcttcc atcccgaaga gttggctcaa gcggagagga
     2581 gcccccgccc cggggagttt ctcgccggtc gcttcgccgt gaaggaggcg ttcttcaaag
     2641 cggtgggaaa cgggatgaaa accctctcca ctcaggatgt gtggaccacg aggggtgaac
     2701 acggcgagcc ggtcatcaac ctctcttcaa aagcccgagc gctcaccgga gagagaacgg
     2761 tcaccctttc gatctcccat gacgatccgg tggccgtcgc cgtcgttctg atttcgggag
     2821 gaaacgatgg cgcgctctga ttggaaaaga cctgcccttg tgccgatcga agcgggcaaa
     2881 cggcggatta aaatgatcct ctcctatgat gggaccccct acagcgggtg ggcatatcag
     2941 accaatgcgc ctacggtggt cggcgtgctc caacaaacaa tccgggagat gatcgacgag
     3001 gaggtcgagg tgatcggcag cgggcggacc gacagcgggg tccatgccgc cgcccaagtc
     3061 tgccacttcg acatcgctca ccggaatatc aaaagcggca agttcaaacc ggcgctgaac
     3121 cgcctgcttc cccaatcgat ccggatcctt cattcggagg aggtcgacgg ctccttccac
     3181 gcccggtata cgacgatggg gcggacctat cgctactact tcaagcggga aggggagctg
     3241 ctgggcttcg atgagcaccg agtggcgaaa ttgaaggcgt ttcccccgct gccgcttctt
     3301 gacggatatg caaagatcct cgtcggaacc cacgatttca ccaccttcgc cgcggcaagc
     3361 gacaaaagtg aaagcaaggt ccgggacatc tatgaatcct attggaagat cgaaaaagac
     3421 cgcttcgggg ccgagttgct cacctatacg atatccggaa acgcctttct gcataagatg
     3481 atccgttcgc tcgtcggttc gatgatccag ttcgccggcc aaaagatggg cgtcgaagag
     3541 tttgttgaga ttctggaggc caaggatcgc tttcgggcgg gtcgaaccgc ccaggcgtgc
     3601 ggcctgtacc tgtaccgcat cacctacgat gaagaggagt atgcgtggtt tgaggaagag
     3661 tacggtggac attgaagatt cgatcagaac atttgtaacg atctgtgatg aagccgaggc
     3721 gctcatttcc gaccgggagg tcgagcccac ctccacaggg gatctttcac cggttctcga
     3781 cctcttcatc actccgagct ttgacaaaaa ccggatcgat cagatgagct tcaagcacct
     3841 cgaagaagcg gtcaggggat gttcgttgtg tcggcttgct gaaaccagaa ccaaaacggt
     3901 cttcgggacg ggggttccct ccccaacgct gatggtcatc ggcgaaggcc cgggggggga
     3961 tgaagacctt cagggtgaag cgttcgtcgg tcgagcggga aagtaccttg atgcgtggct
     4021 caatgcgatc aacctgagcc ggacgaacgg ggtgtacatc accaacatcg tcaagtgtcg
     4081 cccgccgaac aaccgcgatc ctcaaggcga tgaggttgag gcgtgtgttc catatctttc
     4141 ccgccagatt caattgctcc agccccagac aatcctcctg ctcggcagag tcgccgccca
     4201 aacccttctg gggcgaaagg atgctcttgc cgccctgagg ggaacgttcc accgctatga
     4261 aggaattccc gtcctcgtca cctaccaccc cgccgcggtc ctgcgcgacc agcgcctcaa
     4321 gcgcccggtg tgggaggatc tgaagaagat cgcttcgttt ttgaacatcg ctctgccggg
     4381 gagatcctaa tggcgctcta tgccgacgtg ctggtggatg tgccgctgaa gcagtccttt
     4441 acctatctcg ttcccgagga gcttcaggag gcgtccctgt tcggctgccg ggcggtcgtc
     4501 cccttcggct cccgggaggt tacgggatac atcgtcggcg tccgcgacac ggacgattct
     4561 ccgttcgaac tcaaagaact caagcggatg atcgactccg aaccggtgtt caccgataag
     4621 accgtcgagc ttgtccggtg gatcagccgt ttctaccttt gctcggaggg ggaggcgatc
     4681 tccctcgtca ttcccggcgg ccggcgggat tccgcctatg gcgggcttga gcaggatgag
     4741 gcgttcagcg ctgggcggat cgagcggctg agcgccgaac aggaggccgc tctttcaagc
     4801 attttgaact ccgatgaaag gatgttctac ctgcacgggg tgaccggcag cggaaaaagc
     4861 gaagtctatc tccgaaccgc cgaggcggtc atcgccgaag gaaagggggt gatctacctc
     4921 gttcccgaaa tcacgctgac tcaccagctc gccgttctgg tggccggacg cttttcaaac
     4981 cgggtagcga tcctccactc cgccctcacc cccagccagc ggctgaagca atggcgaaga
     5041 atcctcaacg gtgaagtgga cctcgtgatc ggggcccgga gcgcggtctt cgctccggtt
     5101 gaaaacctcg gcctcatcat catcgacgag gagcatgaga acagctacaa aagcgggagc
     5161 accccccgct accacgcccg ccaggtcgcc cagcaccggc cagtgtccca gcgaggccag
     5221 cttcgtgccc a
//
LOCUS       DAOPUZ010000018         5152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_16202_length_5152_cov_3.143810, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000018 DAOPUZ010000000
VERSION     DAOPUZ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16202_length_5152_cov_3.143810"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PKN79_00695"
     CDS             <1..708
                     /locus_tag="PKN79_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94318.1"
                     /translation="IARNMDATYVFQHQDSLLNEEQLELSAGRVKPWGTIHAVLCARE
                     EIHGSFAVINADDYYGKTPYVTLYNHLAGLAPDAKEHAMVGYVLENTTSRIGTVSRAI
                     CSEKGGYLKSMVEHTKIGYEGDSLMSFRESGNLPLTGKEWVSMNFFGFAHSAFDSFER
                     YWHTFIEEHVTSEKNECYLPNGVGNLVAKGEGKVRFYTTDEHWFGMTYAEDRENVKLQ
                     LAKKTEEGLYPEQLWLN"
     gene            complement(705..1625)
                     /locus_tag="PKN79_00700"
     CDS             complement(705..1625)
                     /locus_tag="PKN79_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNZ94319.1"
                     /translation="MKINRYLIIEYFNVIAGSYLTALGIALFSAPSKIVNGGVSGIAI
                     IVHHSLGWDTGFSIFLFSIPLFLLGLLVFGKRYAVKSLAGTILLSVFTTMNNMIFGYE
                     GVLDPSNVLSVLLNSLSAGALMGLGLGMVVRSGSNTGGTDILAQVVARYTPLSMGTSF
                     LIVDGLIILVGALIFGLEMALYALITVFITSAAIDKVVLAFGRRSAKTVFIISTQLEA
                     IKDAILTELGHGGTILSGTGMYTRTDRPVIMTVVANTKLAALTHIVHRYDKNAFMVVQ
                     EAFNVLGEGFTPIEEGAWSSHLDITQEQRP"
     gene            complement(1622..2206)
                     /locus_tag="PKN79_00705"
     CDS             complement(1622..2206)
                     /locus_tag="PKN79_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNZ94320.1"
                     /translation="MRDSIQTLAVFCGSSSGHNPLYTEAAKAIAETMADNNIALVYGG
                     GNRGLMGIVAETVHRRGGYVIGVIPQLLNRADVVMHKVENELIVAHDMHERKALMYER
                     SDGFIALPGGIGTLEELLEVYTWLQLGYHKKPVAVLNIGGFFDHLLDLLTHANGEGFI
                     KDDIMNSLIVADDIPSLFERLDRWDAQLGDKLSP"
     gene            2393..3346
                     /locus_tag="PKN79_00710"
     CDS             2393..3346
                     /locus_tag="PKN79_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94321.1"
                     /translation="MLFAVPVVITWEWLLEDPEVTTFRFQIDSEKPEGWITVDSSVTS
                     YTERGLDGTVDHTLYLQQSYDGIHFSESTRSVAEATILAPEPQAVVEEPVAPKPVTPA
                     PSAPQPAAPVQEVKKAEPVATPPKAPKVKEPSRFYTKLGINLGYNRQRSRFNSAYDIN
                     NLKGGMTLEFHNILTFAKVFGLGVDLDLEYTPYLRGTTYRATLSNVLKGSFDALSTFF
                     TSLEHSFTASPAVMLNVESGRLALGLGGGGFLTYSLDPSFIDSSTLTYRYGVFAKAAL
                     SYRLTDHFTLGLGGKYGVDLDKFASGGHQFLEATMSFGYIF"
     gene            complement(3391..>5152)
                     /gene="rlmKL"
                     /locus_tag="PKN79_00715"
     CDS             complement(3391..>5152)
                     /gene="rlmKL"
                     /locus_tag="PKN79_00715"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HNZ94322.1"
                     /translation="SGQGHHKRGYRGDQTDALLAEHLAFSLLYRSQWRKLADETGSFP
                     TLLDPFCGSGTIVIEAALWATGRAPGLVKARIFPVFNLPIHDVDLYEEIVDEVVSRME
                     EESLSIHGWDRSPKAIRIAEEAAREAGVDHLITFEVKDFTTLTAEDIPPSTGVIVTDP
                     PYGMRMGEWEETRHLYRSIGDALAQFFGGWNVSLLTADDRLLGQIDLKPNRTNAFNNG
                     GIPCTLAHYYIFTHEEKAALRAEWEHRRKERLSQPLSSGAQMAYNRLIKNLKELKPVM
                     EQEGVTSYRIYDADMIEYNAAIDLYEGKWVNLAEYAAPPTVDVEDAERRLNELIDATE
                     RATGVDRESIFVKQRTVQKGLGQYERQAYTDRFYIINENNARYFVNFTDYLDTGIFLD
                     HRPIRKYISEITDHKRFLNLFCYTGTATVQAAKGGALSTVSVDTSTTYLDWARRNMEL
                     NGFGGMNHFYYQSDSISFLWDTFDRYDVIFCDPPTYSNSKDRAPFEVQKDHRSLIKAC
                     MMHLDPGGLLIFSCNYRSFRMDEEVYDEFDVEEITPQTIGKDFSRDPKIHHAFLIRER
                     AKIVVQPQKPKRVIRRKPID"
BASE COUNT         1156 a         1401 c         1415 g         1180 t
ORIGIN      
        1 atcgccagaa acatggacgc aacctacgta tttcaacatc aggattccct gctcaatgag
       61 gagcagctcg aactctcggc gggacgggtc aaaccgtggg gaacgattca cgcggtgctc
      121 tgtgcccgag aagagattca tgggagtttt gcggtgatca acgctgacga ttactatggt
      181 aaaacccctt atgttactct gtataaccat ttagccggcc tcgcccccga tgccaaggag
      241 cacgccatgg tcggctatgt ccttgaaaac accacaagtc gaatcggaac ggtcagtcgg
      301 gcaatctgct cggaaaaggg tgggtacctc aagagcatgg tcgaacacac gaagatcggg
      361 tatgaaggcg attctctgat gagctttcgc gagagcggga accttccgct taccgggaaa
      421 gaatgggtct cgatgaactt cttcggattc gcccacagtg cattcgattc ctttgaacgc
      481 tactggcaca ccttcattga agagcacgtc accagtgaaa agaacgagtg ctaccttccc
      541 aacggagtgg gaaacctcgt agcaaaaggt gaaggaaaag ttcgtttcta caccactgac
      601 gagcattggt tcggaatgac ctacgccgaa gaccgggaaa acgtgaaact tcaacttgcc
      661 aaaaaaactg aagagggact ctatcccgag cagctctggc tcaattaagg ccgctgctcc
      721 tgggtaatgt cgaggtggga actccatgct ccctcctcga tcggtgtaaa cccttcgccg
      781 agcacattga acgcctcttg gacaaccatg aaggcgtttt tatcgtatcg gtggacgatg
      841 tgcgtcaagg cggcgagctt cgtattcgcc acgacggtca tgatcaccgg acggtcggtc
      901 cgagtataca ttccggtgcc ggaaagaatc gtcccgccat gaccgagctc ggtgaggatc
      961 gcatctttta ttgcttccaa ctgcgttgaa atgataaaca ccgtttttgc cgaacgacgc
     1021 ccgaaggcga ggaccacctt gtcgatcgcc gcgctggtga tgaagacggt aatgagggcg
     1081 tagagcgcca tctcaagacc gaagatcaat gctccgacaa ggataatcaa tccgtcgacg
     1141 atgagaaagg aggttcccat cgaaagggga gtatagcggg ccaccacctg ggcaaggata
     1201 tccgttccac cggtgttcga tccgctgcgg accaccattc caagccccaa ccccatcaaa
     1261 gctccggcgc tgagcgagtt taataggacg gagaggacgt tggaaggatc caaaaccccc
     1321 tcgtagccga aaatcatgtt gttcatcgtg gtgaacacgc tgaggaggat tgttcccgca
     1381 aggctcttta ccgcatagcg cttgccaaaa acgaggagcc cgaggagaaa cagggggatt
     1441 gaaaagagga aaatggagaa cccggtatcc cacccgaggc tgtggtgaac gatgatggcg
     1501 attccactga ctccgccgtt cacgattttg cttggtgcac tgaacagggc gattcccaat
     1561 gcggtgaggt atgatcccgc tatgacgttg aaatattcga tgatgaggta gcgattgatc
     1621 ttcatggtga aagcttgtct cccagctgag cgtcccaccg atcgagccgt tcaaaaaggg
     1681 agggaatgtc gtcagcgacg atcagcgagt tcatgatgtc atctttgatg aatccttcgc
     1741 cgttggcgtg agtcagcaaa tcaagcaggt ggtcgaagaa gcccccgata ttcaatacgg
     1801 ctaccggttt tttatggtat ccgagctgga gccaggtata gacctcaaga agttcttcca
     1861 aggtcccgat tcctccgggc agggcgatga atccatcgct ccgctcatac atcaatgctt
     1921 tccgttcatg catgtcatgg gcgacgatga gctcgttttc gaccttgtgc atcacgacat
     1981 ccgctcggtt gagcaactga ggaatgaccc cgatcacata accgcctcgt cgatgaaccg
     2041 tttcggcaac gatgcccatc aacccgcgat tacctccacc atatacgaga gcgatgttgt
     2101 tatcggccat cgtttcggcg atcgcctttg ccgcctcggt gtatagcggg ttgtgtccgc
     2161 ttgacgagcc gcaaaacacc gcaagcgttt gaattgagtc gcgcatctcg gaaaatactc
     2221 ctattctgta tcgatttcgc ctatttactt gttatattaa tgagctgcgt gtattattat
     2281 agtactgaac tacgatcaaa gcgtgtagtc cgtcataggt tacagatagg agttataaac
     2341 catgaagttg aaattcacag cgttgttggc aatcttgatc ctacttcccg ccatgttgtt
     2401 tgctgtcccc gtcgtaataa cgtgggaatg gttgttggaa gatccggaag tcacaacctt
     2461 ccgattccag atagattcgg agaaaccgga aggctggatc accgtcgatt caagcgtcac
     2521 gagctacacc gaacgaggat tggatggaac cgtcgaccat acgctctacc tgcagcagtc
     2581 ctatgacggg atccacttca gcgagagcac ccgatcggta gccgaggcaa cgattcttgc
     2641 accggagccg caagccgttg tcgaggagcc cgttgcaccc aaaccggtca caccggctcc
     2701 ctcagcgcct caaccggcag cacctgtgca agaggtcaag aaggcagagc cggttgccac
     2761 cccgcccaag gccccgaagg tgaaggagcc gagccgcttc tacacgaagc tgggaatcaa
     2821 tctggggtat aatcgccagc gttcccggtt caactccgcc tacgatatca acaacctgaa
     2881 gggcggtatg acgttggagt tccacaacat ccttaccttc gccaaggtgt tcggactcgg
     2941 cgtcgacctt gatctcgagt acaccccgta cctcagggga acaacctacc gagccaccct
     3001 cagcaatgtt ctcaaaggaa gcttcgacgc actttccacc ttcttcacga gtctggaaca
     3061 ctcattcacc gccagtcccg cagtgatgct gaatgttgaa agcggacgtc tggccctcgg
     3121 tctcggaggt ggcggcttct taacctacag ccttgatcct tcgttcatcg acagcagcac
     3181 gctcacctac cgctatggag tgttcgccaa ggcggcgctt tcctaccgcc tgaccgacca
     3241 cttcaccctc ggactcggcg gaaagtacgg tgttgatctg gacaagttcg ccagtggcgg
     3301 acaccagttc ctcgaagcca ccatgtcgtt cggatatatc ttctaagtga tacgttgatc
     3361 atgtaaaggg gccctttcga gggtcctttt tcagtcgatc ggctttcgtc gaatcactct
     3421 ctttggcttt tgtggttgca ccactatttt cgcccgctcc cggatgagaa acgcatggtg
     3481 gatcttcgga tcgcggctga aatcctttcc gatcgtctga ggggtgatct cctcgacatc
     3541 gaattcatca tatacctcct catccatcct gaatgatcga tagttgcagc tgaagatgag
     3601 cagtccgccc ggatcgaggt gcatcatgca cgccttgatc agcgagcgat gatccttttg
     3661 aacctcgaag ggcgcccgat ctttcgagtt ggagtaggtc ggcggatcgc agaagatcac
     3721 atcgtagcgg tcgaacgtat cccagaggaa tgaaatcgaa tcgctttgat agtagaagtg
     3781 gttcattccg ccgaagccgt tcagctccat gtttcgcctc gcccaatcca gataggtcgt
     3841 cgatgtatcg acgctgacgg tggagagggc tcctcccttc gccgcttgga ccgtagcggt
     3901 ccccgtatag cagaagaggt tgaggaagcg tttatggtcg gtaatctcgc tgatgtattt
     3961 tcggatcggg cggtggtcga ggaagatccc cgtatccaga tagtcggtga agttcacgaa
     4021 gtagcgggcg ttgttttcat tgatgatgta gaaccggtcg gtgtatgcct ggcgctcata
     4081 ctggccgagc cctttctgaa ccgttcgttg ctttacaaaa atagactcgc ggtctactcc
     4141 ggttgcccgt tcggtggcgt cgatcaactc attgaggcgc ctttcggcgt cttcgacgtc
     4201 gacggtcgga ggggcggcgt actcggcaag gttgacccac tttccttcgt agaggtcgat
     4261 cgccgcgttg tattcgatca tatccgcatc atagatgcga tagctcgtca ccccctcctg
     4321 ttccatgacc ggcttcagct ctttgaggtt cttgatcagc cggttgtacg ccatctgcgc
     4381 accgcttgaa agcggctggc tgagccgttc ttttctccga tgttcccact ccgccctcag
     4441 agccgctttc tcttcatggg tgaagatgta gtagtgggca agggtacacg ggattcctcc
     4501 gttgttgaac gcattggttc gattcggctt cagatcgatc tgtcctagga ggcgatcgtc
     4561 ggcggtgagg agactcacgt tccatccgcc gaagaattgg gcgagggcat ccccgattga
     4621 gcgatacagg tggcgggtct cctcccactc gcccatccgc atcccgtacg gggggtcggt
     4681 tacgattacc ccggtcgaag ggggaatgtc ttctgccgtc agggtggtga aatccttcac
     4741 ctcgaaggtg atcagatgat cgacgccggc ttctcttgcc gcttcctctg cgatgcggat
     4801 cgccttgggg ctgcggtccc atccatggat ggaaagcgat tcctcttcca tccgggagac
     4861 gacctcgtca acgatctctt catagagatc cacgtcatgg atcgggaggt tgaaaacggg
     4921 aaagatccgg gctttcacga ggccgggagc acgcccggtc gcccagagcg cagcttcaat
     4981 gacgatcgtt cccgagccgc agaaggggtc gagcagggtg ggaaaggagc ccgtttcgtc
     5041 ggcaagcttg cgccactggg agcgatagag gagggaaaag gcgaggtgtt cggcgagaag
     5101 agcatcggtc tgatcccccc ggtatcctcg cttgtggtga ccttgaccgc tg
//
LOCUS       DAOPUZ010000019         5112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_16369_length_5112_cov_3.287325, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000019 DAOPUZ010000000
VERSION     DAOPUZ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16369_length_5112_cov_3.287325"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKN79_00720"
     CDS             <1..470
                     /locus_tag="PKN79_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HNZ94323.1"
                     /translation="KVIGGDVVIATASVRAEGTSREYAPIEFPAVATFEVVEALRDAA
                     IALGKHPHTGVVQCKDSFYGQHDPSKMPVFYELENKWEAYKRLGVLASEMESAALFTV
                     AAYLGVRVGSLFFVVGNQEREKAGLPNPIVHDTSEVIKISVEGIRTLILRDRA"
     gene            complement(448..837)
                     /locus_tag="PKN79_00725"
     CDS             complement(448..837)
                     /locus_tag="PKN79_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94324.1"
                     /translation="MYYHAVKKSSEVLYRTKEEAQRLLFALHAKLTKQHATILDYLLE
                     PQTCQLLLQSKKPIILPAFAINPVEKEKLLWYFSSLGSKGKTYPYSGLHECYFLSTCF
                     CELGKVTADPPPYPLKEVLAVKHGRAE"
     gene            953..1543
                     /locus_tag="PKN79_00730"
     CDS             953..1543
                     /locus_tag="PKN79_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HNZ94325.1"
                     /translation="MHMAQQIQDLVASIRKDGVEAAKKEAASILQSAEEQAQTIIAQA
                     QEKATQIVSEAEEAIALANRRAEKALSQAGRDLLLSLKASIEGQFSRILVEKTSKALS
                     SKDFIGLLTKVLEAGDGDVNIEIDPKAFEALADELQTALAAKLKSGLVLKPHDDVESG
                     FRLVAKDGSYFYDYKASELADLLQPYLARLLHERIF"
     gene            1553..2086
                     /locus_tag="PKN79_00735"
     CDS             1553..2086
                     /locus_tag="PKN79_00735"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HNZ94326.1"
                     /translation="MASYYYFATTLPLLRLHQKSPITVELFKAQAEKHLNSRDWKTLK
                     SGLSGTPSKHAMLTSYRSYASALNTALARKRQVRLGFAIDEDLHRQTFPKEIETVTDE
                     LLERSDPLEAEMQFITLLWKRIDRLVGMKVFEFDALLGYGLKLLLLERVNIFTQEVGQ
                     ATFETLLGTLRSEIDQM"
     gene            2098..3819
                     /locus_tag="PKN79_00740"
     CDS             2098..3819
                     /locus_tag="PKN79_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNZ94327.1"
                     /translation="MARNTGRVLGVNGNMVNVEFDGAIAKNEVGYIHLGDIRLKGEVI
                     RINGNIAALQIFEMTGGIVVGDEVEFTGEMLSVELGPGLLTQIYDGLQNPLPELARQC
                     GFFLQRGVYLQALPNVDWEFTPTVSKGDIVKAGDTIGTVPEGLFTHQIMVPFDLVGSF
                     TVTSIKQAGTYKTRDIICTVEDSKKKKLELSMVFSWPVKKAIRLYSERLRPVEPMMTK
                     IRIIDTLLPVAKGGTFCTPGPFGAGKTVLQHMTSRNAEVDIVIIAACGERAGEVVEIL
                     KEFPELIDPKTGRSLMERTIIICNTSSMPVASREASVYTAVTIAEYYRQMGLDILLLA
                     DSTSRWAQAMREMSGRLEEIPGEEAFPAYLESRIAEFYERAGIVKLSDGRTGSVTIGG
                     TVSPAGGNFEEPVTQATLKVVGAFHGLSRERSDARKYPAIDPLTSWSKYPGIIKQSEV
                     NQMRRVLHGSHEVNQMMKVVGEEGTSINDYVTYQMGELLDSCYLQQNSFDPIDTSVPP
                     ERQKHVFAKLHRLMNTPLTFETKSDVRRVFNNLRQLLLDWNNTRWGSDEFKAAEAALD
                     EAFKALG"
     gene            3831..>5112
                     /locus_tag="PKN79_00745"
     CDS             3831..>5112
                     /locus_tag="PKN79_00745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNZ94328.1"
                     /translation="MQKIYSKIESIVGNVITVRAEGVANGELAVVTSSGSTSFANVIK
                     LDGDLVSLQIFSGATGTSTGDHVRFLGNPMRVSSSPNLLGRVFDGTGSPRDGKPELKE
                     EMIDIGGPSVNPAKRIIPRKMIRTGIPMIDLFNTLVESQKLPIFSVSGEPYNELLARI
                     ALQAEVDLIILGGMGLKFDDYLYFKDTLEEGGAMDRTIMFVHTASDPTVECMLVPDMA
                     LAVAEKFALEGKKVLVLLTDMTNFADAMKEMAITMDQVPSNRGYPGDLYTQLASRYEK
                     AVDFEGAGSVTILAVTTMPGDDVTHPIPDNTGYITEGQYYLKGGRIEPFGSLSRLKQQ
                     VNSATRDDHRALMDGMIRLYASFKEALEKQSMGFMMSEWDEKLLKYGDLFEEKMMDLS
                     VNIPLEAALDLGWEILGDCFEPNETGLRSDLIRAR"
BASE COUNT         1207 a         1362 c         1469 g         1074 t
ORIGIN      
        1 cgaaggtgat cggcggcgac gtggtcatcg caaccgccag cgtccgggcg gagggaacca
       61 gccgcgagta cgccccgatc gagtttcccg ccgtggcgac cttcgaggtc gtcgaagcct
      121 tgagggatgc ggcgatcgca ctggggaaac acccccacac cggcgtagtg cagtgcaaag
      181 actcctttta cggtcagcac gatccatcca agatgccggt cttctacgag ctggagaaca
      241 aatgggaggc gtacaagcgc ctgggagtcc tggccagcga gatggaaagc gccgccttgt
      301 tcaccgtcgc cgcctacctc ggcgtccggg ttgggtcgct cttcttcgtc gtcggaaacc
      361 aggagcggga gaaagcgggc cttcccaacc cgatcgtcca cgacaccagc gaagtgatca
      421 aaatctcggt ggaagggatt cgaaccctca ttctgcgcga ccgtgcttga cggcgagcac
      481 ctccttcagc ggatagggtg gaggatcggc tgttaccttg cccagctcgc aaaagcacgt
      541 cgaaaggaaa tagcactcat gcagcccgct gtacggatag gtctttcctt tcgatccgag
      601 ggaagagaag taccacagga gcttctcttt ttcaaccgga ttgatcgcaa acgccggaag
      661 gatgatcggt ttctttgatt gcagcagaag ttgacacgtt tggggttcca gcaggtagtc
      721 gagaatcgtt gcatgctgtt tcgtcagctt tgcatggagg gcgaacagga gcctctgggc
      781 ttcctccttt gtgcgataga gcacctcgga tgatttcttg acggcgtggt aatacatggc
      841 agcctccacc cggtaagggg aatctcttgg ggtttcactt cagggcactt gagcttttat
      901 tcccattgag tgataatgtt gtcggtttca ttaacgtatc agagaaggta ggatgcacat
      961 ggcccaacag attcaagatt tggttgcttc gatccgaaaa gatggggttg aagcggcaaa
     1021 aaaggaagcg gcttccattc ttcaatcagc ggaagaacaa gcacaaacaa tcattgctca
     1081 agcgcaggaa aaagcgactc agatagtttc tgaagcggaa gaggccattg ctctggcgaa
     1141 ccggcgggct gaaaaagcgc tttctcaggc cgggcgcgat cttcttcttt ccttgaaagc
     1201 ttcaatcgag ggtcagtttt cccgcatcct tgttgaaaaa acatcgaaag cgctgagcag
     1261 caaggacttc atcggattgt tgacgaaagt tcttgaggcc ggcgacgggg atgtgaacat
     1321 cgaaatcgac cctaaggctt ttgaagccct tgccgatgag cttcaaacgg cgttggcagc
     1381 caaattgaag agcggtctgg ttctgaaacc ccatgatgac gtggaatcgg ggttccgcct
     1441 cgttgcgaaa gacggctcgt acttctatga ctacaaggcg agtgaactgg ctgacctctt
     1501 gcaaccgtac cttgctcgac tgttgcacga gcgaatcttt tagggtccgg cagtggcatc
     1561 atattattat tttgcaacaa cgctgccgtt gctccggctt catcaaaagt cacccatcac
     1621 cgttgagctg ttcaaagcac aagcggaaaa acaccttaac tcacgtgatt ggaagactct
     1681 gaaaagcggg ttgagcggaa cgccaagcaa gcatgcgatg ctgacatcat accgaagtta
     1741 tgcctccgct ttgaatacgg ccctcgccag gaaacggcaa gtgcggctcg gttttgccat
     1801 cgatgaagat cttcatcgtc aaacgttccc caaagagatt gaaaccgtaa ccgatgagct
     1861 gcttgaacgt tccgatccgc tggaagcgga gatgcagttc atcacgctgc tctggaaacg
     1921 gatcgaccgg ttggtcggaa tgaaggtctt cgagttcgat gccctcttgg gatacggact
     1981 taaattgctt ttgttggaac gggtgaacat cttcacccaa gaggtcggcc aggcgacctt
     2041 tgaaaccctc cttggcacat tgagatcgga aatagaccag atgtagtgga gtaggatatg
     2101 gcaaggaaca caggacgtgt gctcggcgtg aacggcaaca tggtgaatgt cgagtttgac
     2161 ggagcgatcg ccaaaaacga agtgggatac atccatcttg gggatattcg cctcaaaggc
     2221 gaggtgatcc ggatcaacgg caacatcgcc gctctgcaga tcttcgagat gaccggtgga
     2281 atcgttgtag gcgacgaggt tgagtttacc ggcgagatgc tcagcgtcga gctggggccc
     2341 gggctcttga cgcagatcta tgacggactt cagaacccct tgccggaact cgcccggcaa
     2401 tgcggcttct tccttcaacg gggtgtatac cttcaggctc ttcccaacgt tgattgggag
     2461 ttcaccccga ccgtatccaa aggtgacatc gtcaaggcgg gggatacgat cggaaccgtc
     2521 ccggaaggat tgttcaccca ccagatcatg gttccgtttg atcttgtcgg ttcgttcacc
     2581 gtaacctcaa tcaaacaagc ggggacgtac aaaacccgcg atatcatctg cacggtcgag
     2641 gactccaaaa aaaagaagct tgagctgtcc atggtcttca gctggccggt gaagaaagcg
     2701 attcggcttt acagcgaacg actgaggccc gtcgagccga tgatgacgaa gatcagaatc
     2761 atcgacaccc tgcttccggt cgccaagggc ggaacgttct gtactcccgg accgttcggt
     2821 gccggaaaaa ccgttcttca gcatatgacg agccggaacg ccgaagtcga tatcgtcatc
     2881 atcgccgcct gtggcgagcg ggccggtgag gtcgtcgaga ttctgaagga gttccccgaa
     2941 ttgatcgacc cgaaaaccgg ccgttcgctg atggaacgga cgatcatcat ctgcaacacc
     3001 tcctcgatgc cggtcgccag tcgggaagcg tcagtgtaca cagccgtaac gatcgccgaa
     3061 tactatcgac agatgggtct tgacatcctg ctgcttgccg actccaccag ccgctgggct
     3121 caggcgatgc gggagatgag cggccgcttg gaggagattc ccggtgaaga ggcgttcccg
     3181 gcttacctgg aaagccgaat cgccgaattc tatgaacgag ccggaatcgt caagctttcc
     3241 gatggaagga ccggctccgt tacgatcggc ggaacggtca gtcccgccgg cggaaacttc
     3301 gaagagccgg taactcaagc gacgctgaaa gtcgtcggag ccttccacgg cctcagccgc
     3361 gaacgctccg atgcccggaa gtatccggct atagaccctt tgacatcttg gtcgaagtat
     3421 ccgggaatca tcaagcaatc cgaggtgaat cagatgcggc gggttctgca tggttctcat
     3481 gaagtcaatc aaatgatgaa ggtcgtcggt gaagagggaa caagcatcaa tgactacgtg
     3541 acctaccaga tgggcgagtt gctcgacagc tgttatcttc agcagaactc cttcgatccg
     3601 atcgacacat cggtgccgcc ggagcgccag aagcacgtct ttgcaaagct ccatcggctg
     3661 atgaacacgc cgttgacctt tgagacgaag agcgatgtcc gccgagtctt caacaacctc
     3721 cggcagcttc tcctggactg gaacaacacg aggtggggat cagacgagtt caaagcggcg
     3781 gaagccgccc ttgatgaagc attcaaggct cttgggtaag ggagaagagc atgcaaaaaa
     3841 tatattcgaa gattgagtca attgttggaa acgtcatcac ggtccgagcc gaaggagttg
     3901 cgaacggcga gctcgccgtc gttacgagca gcggatcgac ctcgttcgcg aacgtgatca
     3961 agctggacgg ggatcttgtc tcattacaga ttttcagcgg agccaccggg acatcgaccg
     4021 gcgaccacgt ccgctttctg ggcaatccga tgcgggtctc ctcatcgccc aacctcctgg
     4081 gacgggtgtt cgacggaacc ggatcccccc gggacggcaa gccggaactg aaggaagaga
     4141 tgatcgatat cggcggtccc tcggtaaacc cggcgaagcg gatcattccc cgaaagatga
     4201 ttcgaaccgg tattccgatg atcgacctgt tcaacaccct tgtcgaaagc cagaagcttc
     4261 cgatcttctc cgtcagcggc gagccgtaca acgaactgct tgcgcgaatc gctctgcagg
     4321 cggaggtcga tctgatcatc ctcggcggca tggggctgaa attcgacgac tacctctact
     4381 tcaaggatac tctggaagag ggcggggcga tggatcggac gatcatgttc gtccataccg
     4441 ccagcgaccc gaccgtcgaa tgcatgctgg ttcccgacat ggctctcgcc gtcgccgaga
     4501 agttcgcctt ggaaggaaag aaggttcttg tcctgttgac ggacatgacg aactttgccg
     4561 atgcgatgaa ggagatggcg atcacgatgg atcaggttcc ttccaaccga ggatatcccg
     4621 gagacctgta cacccagctc gcctcccgct atgagaaagc ggtcgacttc gaaggagcgg
     4681 gttccgttac gatccttgcc gtcaccacga tgcccggtga tgacgtgacc cacccgattc
     4741 ccgataacac cggctatatc actgaagggc aatactatct caagggcgga agaatcgaac
     4801 cgttcggctc gctttcccgt ctgaagcagc aggtcaacag cgccacccgg gatgaccacc
     4861 gggcattgat ggacggtatg attcggctct acgcctcctt caaggaagcc ctcgaaaagc
     4921 agtcgatggg cttcatgatg agcgagtggg atgaaaaact gctgaaatac ggtgatctct
     4981 ttgaagagaa gatgatggat ctttcggtga acatccccct tgaagcggcg ctggatctgg
     5041 gctgggagat tctcggcgat tgtttcgagc ccaacgaaac cggactgcgg tccgatttga
     5101 ttcgagcccg ct
//
LOCUS       DAOPUZ010000020         5015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_16791_length_5015_cov_2.840726, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000020 DAOPUZ010000000
VERSION     DAOPUZ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16791_length_5015_cov_2.840726"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /gene="rpmA"
                     /locus_tag="PKN79_00750"
     CDS             complement(<1..171)
                     /gene="rpmA"
                     /locus_tag="PKN79_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNZ94329.1"
                     /translation="MASKKGGGSTRNGRDSKAKRLGVKRYGGQLVKAGEVLVRQRGTK
                     FHPGENVGLGSDY"
     gene            complement(184..498)
                     /gene="rplU"
                     /locus_tag="PKN79_00755"
     CDS             complement(184..498)
                     /gene="rplU"
                     /locus_tag="PKN79_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNZ94330.1"
                     /translation="MYALVEILGKQYKAIEGETVQVDYISKQEVGSAIEYATVLAVVD
                     EKNARFGTPYVENAKVKVTLVENIKGDKIRVFKKKRRKGYRKTQGHRQWYSLVTIDKI
                     IA"
     gene            642..1199
                     /locus_tag="PKN79_00760"
     CDS             642..1199
                     /locus_tag="PKN79_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ94331.1"
                     /translation="MKEQLILSMLSLASEQGLGNVSLSRLANENGISKATIFHHFTGR
                     AHLIEEVFAYCHRLALKRMRTIDLEGNAEEVLLRALDHWSELYETEPLYSFYRIVEQE
                     SWTNGQARTIKQTFDEMLIAQSSVLIEHLEERGKLRIDDLELAIETFSATIARHLVRT
                     LLTDEEIRYQAERFIRRFCTHYAPT"
     gene            complement(1192..2919)
                     /gene="argS"
                     /locus_tag="PKN79_00765"
     CDS             complement(1192..2919)
                     /gene="argS"
                     /locus_tag="PKN79_00765"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNZ94332.1"
                     /translation="MLRAIRAGWKARIEEELYKIAETNGKVSLPELIVQTPPKVELGD
                     IAFPLFAQAKVLGMSPPALANELKNRLDAQELLVSGPYLNVRIDMNALALDLTKAIRK
                     EGDAYGHNRTLGGKRIVVEFSCPNTKKPLHLGHLRNDSIGESISAILKANGAEVRKVN
                     LINNRGVHICKSMLAYRKFGNGETPESTGKKGDHFVGDYYVLYNRKAEEDPSLEGQAQ
                     AMLKAWEDGDEEVVALWEKMNRWTLEGLSQSYERMRVSFDHYYYESETYRYGRDEVLK
                     GVEQGVFYREADNSVWVDLEAIGLDKKVLLRSDGTSLYMTQDLGTAIRRYEDWSFDSM
                     IYVVASEQQYHFKVLFHALGLLGYPWAKDLHHLSYGMVNLPDGKMKSREGKVVDGDDL
                     VEELTLLAKEEIIAKEREELVGDLEETSLSIALGALNYYLLQVSPTKDMIFNPAESIS
                     FNGNTGPYLQYMGARISSMLAKYEQEIPASFDASVLTLEDERSLIRQLALFGEVVERA
                     AEGYDPSQITAYLYELSRTFSRWYHDNPVLKASTPALVEARVVLASNVLQVLKNAFAL
                     VGIPFLAAM"
     gene            complement(2959..3915)
                     /locus_tag="PKN79_00770"
     CDS             complement(2959..3915)
                     /locus_tag="PKN79_00770"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNZ94333.1"
                     /translation="MSRKTEVLARLQQALNPVSGERIASDLSISRAAVWKAIEGLRKD
                     GYLIEGVPNQGYRLSSVQPLLDQSKLFSLLPSYRIEGVDMIDSTNSELKRRNPRVKTL
                     LFANTQSGGRGRLGRSFFSPRGGVYFSLALPLNVSIDSALLVTSIAAVATAEALSEIA
                     HVETSIKWVNDLYLGDKKLCGILTEGIISMETKTLATVIIGIGINLHLPIDQFPTELQ
                     AIATSAFDSERPLSIDPNRLVQGIVTNIERLIEDLPDRSFLEIYRRRSNLIGKTVQVH
                     EGAHSHPARVLGIDDDAHLIIADETGRTRSLSSGEVSIRLEG"
     gene            3998..4513
                     /locus_tag="PKN79_00775"
     CDS             3998..4513
                     /locus_tag="PKN79_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNZ94334.1"
                     /translation="MNERRILLVAFFTALIIVGSFIRIPLPPVPITLQTLFVLLAGYM
                     GGPEVALMSVGIYLFLGAIGLPVFSGGGGFALLFGPTGGYLLALLPAALIAGFGGFKR
                     IWRMVLFGLLATVVIYLGGVSWLAHHLALSFGQAIAVGMLPFLVGDGIKLGLAIALAR
                     RFSERVRSLLA"
     gene            4543..>5015
                     /locus_tag="PKN79_00780"
     CDS             4543..>5015
                     /locus_tag="PKN79_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNZ94335.1"
                     /translation="MEPCKDHPFDSTVFIPSFLKTFDRIAGKANFHGLKPLPRMEIVG
                     QLCDELLELFFPGRCGREQARMNLEEYLAYQMDVVTSHLKGQLYLAFRYDDETLDSEA
                     AEERSDRAVRSVASRFPSIRLSLKADAQAAYEGDPAARNIREVILSYPCIKSLTI"
BASE COUNT         1053 a         1457 c         1302 g         1203 t
ORIGIN      
        1 atagtcagag cctaagccga cgttctcgcc ggggtggaac ttcgttcctc gttgacgtac
       61 gaggacttca ccggctttga ccaattggcc gccatagcgc tttacaccaa gccgcttcgc
      121 tttgctgtcg cgtccgttgc gggttgaacc gccacctttc tttgatgcca tcttcagtcc
      181 tccttatgca atgatcttgt cgatcgtcac gagggaatac cactgacggt gtccctgggt
      241 cttgcgatag cccttccggc gcttcttctt gaacaccctg attttatccc ccttaatgtt
      301 ttcaacgagg gtcactttga ccttggcgtt ctcaacatac ggagttccaa agcgggcatt
      361 cttctcatca acgactgcaa gtaccgtcgc gtactcgatg gcgctgccga cttcctgctt
      421 gctgatataa tcaacttgga ccgtctcgcc ttcgatcgct ttgtactgct ttccgagaat
      481 ttctactagt gcgtacattt cgctttcttc cttaccagtg attgtcgaac ccttatgtac
      541 catgattaca agtaaaaatg caagtgattt tctaaataac tcttgtattt ccaaagaaaa
      601 gcctcttgac tccctaccga acggtcggtt aatatgcgcc tgtgaaggaa caattgattc
      661 tcagcatgct ttctctggcc agtgagcagg gacttggcaa tgtcagcttg agtcggctgg
      721 caaatgaaaa tggaatcagc aaagcgacga tcttccacca cttcaccggc cgagcccatc
      781 tgatcgaaga ggtattcgcc tattgccatc gcctggctct gaaacgaatg aggactatcg
      841 atctggaggg aaacgccgaa gaggtgcttc ttcgagctct cgaccactgg agcgagctct
      901 atgaaaccga gccgctctac tccttttacc ggatcgttga acaggaatca tggacaaacg
      961 ggcaggcgcg gacgatcaag cagaccttcg atgagatgct catcgcccaa agctccgtgc
     1021 tgatcgaaca tcttgaagaa cggggaaagc tccggatcga cgatcttgaa ttggcgattg
     1081 aaacattcag tgcaacaatc gctcgacatc tggttcgaac gctccttacc gatgaagaga
     1141 ttcgctacca agccgagcga ttcattcgtc gtttctgtac ccactatgcc cctacatagc
     1201 agcgaggaag gggattccaa ccaaagcaaa cgcgtttttc aggacctgaa ggacatttga
     1261 agcaagaacc acccgcgctt cgacaagggc gggcgtggac gccttgagca ccggattgtc
     1321 atggtaccac cggctgaacg tcctactcag ttcgtagaga taggcggtga tctgggaggg
     1381 atcatacccc tcggcggcgc gctcgaccac ctcgccgaag agcgcaagct ggcggatcag
     1441 ggagcgctca tcctccaagg tgaggacgct cgcgtcgaag gaagcgggaa tctcctgctc
     1501 atacttggcg agcatgctgc tgatccgagc ccccatgtat tggagatagg gaccggtatt
     1561 tccgttgaag gagatcgatt cggcgggatt gaagatcatg tccttcgtcg gagagacttg
     1621 gagcagatag tagttcaaag ctccgagggc gatcgagaga ctcgtttctt caagatcgcc
     1681 gacaagctcc tcccgttcct tggcgataat ctcctccttt gccaggaggg taagctcttc
     1741 aaccaaatca tccccgtcga ccaccttccc ttcccggctc ttcatcttcc cgtcgggcag
     1801 gttcaccatc ccgtaggaga gatggtgcaa atccttcgcc cacggatagc caagcaggcc
     1861 gagggcatgg aagaggactt tgaaatggta ctgctgctcg ctggcgacga catagatcat
     1921 cgagtcgaag gaccaatcct catatcgcct gatcgccgtc cccagatcct gagtcatata
     1981 caatgacgtg ccgtcactgc gaagcagcac tttcttatcc agaccgatcg cttccagatc
     2041 cacccacacc gagttgtcgg cttcccgata gaagaccccc tgctcgaccc ctttgaggac
     2101 ctcatccctg ccgtagcgat aggtttccga ttcatagtag tagtggtcga agctcaccct
     2161 catccgctca tagctttggg aaaggccttc cagcgtccat cggttcatct tctcccaaag
     2221 cgcaacgacc tcttcatccc cgtcctccca tgccttgagc atcgcctgag cctgcccctc
     2281 caacgaagga tcttcttcgg ccttccggtt gtagaggacg tagtagtctc cgacgaagtg
     2341 atcgcccttt ttcccggtgg actcgggggt ctccccgttg ccgaacttcc gataggcaag
     2401 catcgatttg cagatgtgca ccccgcggtt gttgatcaaa ttgaccttcc gaacctccgc
     2461 tccgttcgcc ttgagaatcg ccgagatcga ctcgccgatc gagtcgttgc gcagatgccc
     2521 gaggtggagc ggtttcttcg tgttgggaca ggagaactcg acgactattc gctttccacc
     2581 gagcgttctg ttgtgaccgt aggcatcacc ttctttcctg atcgccttcg tcagatcgag
     2641 ggcaagggcg ttcatgtcga tgcggacgtt gaggtacgga ccggaaacga ggagttcttg
     2701 ggcatccaag cggttcttca gttcatttgc cagcgccggc gggctcatcc ccaagacctt
     2761 cgcctgggcg aaaaggggaa acgcgatatc cccgagttca accttggggg gcgtctgaac
     2821 gatcagttcg ggaaggctca ccttcccgtt cgtttcagca atcttgtata attcttcttc
     2881 aatgcgggct ttccaacccg cgcgtattgc tctgagcatc gtatcttcct tctggaagca
     2941 tcctacaata aaggctccct acccttcaag gcgaatgctg acttctccgc ttgaaaggct
     3001 gcgggtcctt cccgtttcat cggcaatgat cagatgggcg tcatcatcga ttccaagaac
     3061 cctggccgga tgggaatgag cgccttcatg aacctgaacg gtttttccga taaggttgct
     3121 tcttcttcgg taaatctcca ggaaagaacg atcaggcagg tcttcgatca atcgttcaat
     3181 attggtgacg attccttgca caagacggtt cgggtcgatc gagagcggcc gttcgctgtc
     3241 aaaggcgctg gtagcgatcg cctgcagctc ggtcgggaat tgatcgatcg gcagatgaag
     3301 gtttatcccg atgccgataa tcaccgtcgc aagcgtcttc gtttccatcg agatgattcc
     3361 ttcggtcagg atgccacaga gttttttgtc ccccagatac agatcgttca cccatttgat
     3421 cgaggtctcg acatgagcaa tttcgctcaa tgcctccgcc gtggccaccg cagcgattga
     3481 ggtgacgagc aatgcggaat cgatgctcac gttcaaggga agagccagac tgaaatacac
     3541 ccctccccgc ggagagaaga acgacctgcc gagccttccc cgtccgccgc tttgtgtgtt
     3601 ggcgaagagg agggtcttca ctctggggtt tcgccgcttc agctcgctgt tggtcgaatc
     3661 gatcatatcg actccctcaa ttcgatagga aggcagaagg gagaagagct tggactggtc
     3721 aagcagcggt tgaaccgaag aaagccgata cccctgattg ggaacaccct cgatgagata
     3781 tccatccttt ctcagaccct caatcgcttt ccacaccgca gctcgggaga ttgacagatc
     3841 ggaggcgatc ctctcccccg ataccggatt caaagcttgt tggagacggg caagcacctc
     3901 tgtttttctg ctcatacgat gagcataaaa agtgattgta ccattgtcaa ccttgttatg
     3961 gtttacaacc tgccgatctt tgtgctatcg tcccgccatg aacgaacgcc gaatcctcct
     4021 tgtcgccttt ttcaccgccc tcatcatcgt cggatccttt atccggattc cgcttccgcc
     4081 ggttccgatc accctgcaga ctctcttcgt gctgcttgcc gggtatatgg gagggccaga
     4141 ggtcgccctc atgagcgtcg gcatctatct tttcctcggt gcgatcggcc tgccggtctt
     4201 cagcggcggc ggcggtttcg ccctgctgtt cggaccgacc ggaggctacc tccttgcgct
     4261 gcttcccgca gccctgattg ccggcttcgg cggctttaag cggatctgga ggatggtcct
     4321 cttcggcctg ctggcaacag tcgtgatcta tctcggggga gtatcgtggc ttgcacatca
     4381 tctcgcgctt tcctttggcc aagcgattgc agtgggaatg cttcccttcc tggtcgggga
     4441 cgggatcaag ctgggattgg cgatcgccct cgctcggcgt ttttcagaac gggtgcgctc
     4501 ccttcttgcc taaaggaagc gagaacccgt aagctgttgg tcatggaacc atgcaaagat
     4561 catccttttg actcaacggt attcatccct tcatttctga agaccttcga ccggatcgcc
     4621 gggaaggcga actttcacgg cctgaagccg ctgccgcgaa tggaaatcgt cggccagctc
     4681 tgcgacgaat tgctggaact cttcttcccg ggacggtgcg gaagggagca ggcccgaatg
     4741 aacctagagg agtatctggc ctatcagatg gatgtggtca ccagccacct caaaggccag
     4801 ctctacctcg ccttccgcta cgatgatgaa acgctcgact ctgaagcggc ggaggagaga
     4861 agcgaccggg cggttcgaag cgtcgcttcc cgcttcccct cgattcggct cagcctgaaa
     4921 gccgatgccc aggccgccta cgaaggcgac ccggccgccc gaaacatccg ggaggtcatc
     4981 ctctcctacc catgcatcaa aagcctgacg atcca
//
LOCUS       DAOPUZ010000021         4939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_17138_length_4939_cov_2.807125, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000021 DAOPUZ010000000
VERSION     DAOPUZ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17138_length_4939_cov_2.807125"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PKN79_00785"
     CDS             <1..912
                     /locus_tag="PKN79_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNZ94336.1"
                     /translation="LDDITHIQFLEDTLGLFPETICFRYNPGPERTGNAIIGNPVEAK
                     YGVTSSQIIDCYRIAKKKGAKRFGLHTMVASNELDGTYIVETARMLFELCVRIQKEAG
                     VRISFIDMGGGIGIPYRPEDSAMDLAFVSREMKKLYEQIIVPAGLDPLSITFEVGRMI
                     TGPYGYLVSKVLHVTEKYKDYVGLDASMADLMRPALYGAYHHITVLGKDREKHDSVYD
                     VTGSLCENNDKFAIDRSLPKVEVGDVVVIHDAGAHGHAMGFNYNAKLRSAEYLLKKDG
                     TIRLIRRAQTYDDYVATLRFDGAPVRI"
     gene            914..1327
                     /locus_tag="PKN79_00790"
     CDS             914..1327
                     /locus_tag="PKN79_00790"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HNZ94337.1"
                     /translation="MHIFPVRVYYSDTDCGGIVYHARYLDFAEHARTELLREIAQRHR
                     FDGNQSAMLNLSKTGFVVKSVTIDYRKPAKLDDLLEVRTTVTSMKRFSMTLTQQVFKD
                     EDLIADLLVKVAAIHLDTLKPQPLGDDLQKAFLGG"
     gene            1329..1916
                     /locus_tag="PKN79_00795"
     CDS             1329..1916
                     /locus_tag="PKN79_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNZ94338.1"
                     /translation="MAFTVYKGAAESSLPIALVPLWGEYPNVEGSVRLGLRDTTLLLI
                     FKMRSPQLLRMVDRHNGPVYRDSCVEAFLQQQGRDEYLNFEFSASGSILAAKGKNREN
                     RTFFDPDLIDTIPRTIEILENNNRQSRYVVSVELDLRAFGLVEEGREVNLRGNFNACG
                     DGHKVPYYLAANPIGTVKPDFHVPDFFAPLIITGR"
     gene            complement(1903..2676)
                     /locus_tag="PKN79_00800"
     CDS             complement(1903..2676)
                     /locus_tag="PKN79_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNZ94339.1"
                     /translation="MRISRLLGDSAIKTLHDKTVTVVGLGAVGGMALESLVRSGVGKV
                     RIVDFDTIGITNLNRQILATYDTIGSEKTQVAKERIGAINPSCEVEVLPLFVNKESVH
                     RIFEGKETDLVIDAIDSLSPKVALLEYAYKANIPAISSMGAALRRDPFLVRKADLMDT
                     WGCPLAKQVRYRLKRRGVGRGIDVIFSAEEIRFIYKDPSEEEHADFNERISQTGRRRN
                     VLGSLPTVTAIFGQHLAHLALTKLLGEAELSGVEVFNAR"
     gene            complement(2666..3313)
                     /locus_tag="PKN79_00805"
     CDS             complement(2666..3313)
                     /locus_tag="PKN79_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNZ94340.1"
                     /translation="MFDLHRHLSSASPSHACFYATSRKEEWETVIHAPYGAIGLLPSQ
                     LPDGVEELHALLVAHPHLQIGEVGLDRRFPHPDRQESFLKELLRVAVELERSVTLHCV
                     RREGRLLQILRGIERLPRLLWHSFGGSYEVAREAARLDIILSYGPRLKHSKLSGEGKR
                     LTRLPFGLESDYEKPTEDGYPETWQAHQRWFAELTGWSLDRLVRNNDEKRTILAH"
     gene            complement(3315..3623)
                     /locus_tag="PKN79_00810"
     CDS             complement(3315..3623)
                     /locus_tag="PKN79_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNZ94341.1"
                     /translation="MKTFVVPILVSALATFIPRVIPYWVSFLDKLPPLLSRMLRLLPI
                     AAFGPLIFPGVFLDYVPHLYSGFAGISAAFILAYFKGGMILPILSSIVVTYLALLLGA
                     "
     gene            complement(3620..4315)
                     /locus_tag="PKN79_00815"
     CDS             complement(3620..4315)
                     /locus_tag="PKN79_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNZ94342.1"
                     /translation="MSERNLTLSTGCKDGFPIFIGYFPTAMAFGLVVKNLGLRLWEAV
                     LFSVTNFAGSGQFLAASLIGSGAFLAELFISVLLINLRYTFMGAEVARRLEDGITGMK
                     RALIAHGTTDEVFSISLMNPGRLPASYMAGLELTSYAGWVSGTALGFLVGLVLPPALQ
                     LAVGVTIYAMFSSLLAQEFRKKGIKVLLIAAISAGTHSVLTLLLGVKIGWSFVVSMLL
                     ASFIGSLIIREDV"
     gene            complement(4334..>4939)
                     /gene="msrA"
                     /locus_tag="PKN79_00820"
     CDS             complement(4334..>4939)
                     /gene="msrA"
                     /locus_tag="PKN79_00820"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNZ94343.1"
                     /translation="TEIICFTCGAHLGHVFEGEGFTPTNTRHCVNSISMVFRPGEPVT
                     TALYAGGCFWGTQYLFSKKSGVRTAESGYSGGTTENPTYQEVLTGKTGHYETVKVTYD
                     PLKITYEELTKYFLEIHDPGQVDGQGPDIGKQYESVIFYRSRDQFDTALKLIRRLETG
                     GMKVATRVLPQKMFYRAESYHQDYYRKKGTLPYCHYWTKRF"
BASE COUNT         1127 a         1367 c         1423 g         1022 t
ORIGIN      
        1 ctggatgaca tcacccacat ccaattcctt gaggataccc tgggcctgtt ccccgaaacg
       61 atctgtttcc ggtacaaccc cggtcccgag cgcaccggaa atgcgatcat cggcaacccc
      121 gtcgaggcga agtacggagt tacctcgagc cagatcatcg actgctacag gatcgccaaa
      181 aagaaggggg cgaagcgctt cggcctccat acgatggtgg cttcaaatga actggacggc
      241 acctatatcg tcgaaaccgc ccggatgctc ttcgagctct gtgtcaggat tcagaaggag
      301 gcgggggtga gaatctcctt catcgacatg ggcgggggaa tcgggattcc ctatcgaccg
      361 gaagactcgg cgatggatct tgccttcgtt tcacgggaga tgaagaagct ctatgaacag
      421 atcatcgttc ctgccggcct cgatccgctt tccatcacct tcgaggtcgg aaggatgatc
      481 acggggcctt acggctatct ggtcagtaaa gttctccacg tgacggagaa gtacaaggac
      541 tacgtcggcc tggatgcatc gatggcggat ctgatgcggc cggcgctgta tggggcatac
      601 caccacatca ccgtcctcgg caaggatcgg gaaaagcatg acagcgtcta tgatgtcacc
      661 ggctcgctct gtgaaaacaa cgacaagttc gcgatcgacc gaagccttcc gaaggtcgag
      721 gtgggggatg tcgttgtgat ccacgatgcc ggggcgcacg ggcatgcgat ggggttcaac
      781 tacaacgcaa aactccgcag cgcagagtat cttttgaaga aagacggcac catccggttg
      841 attcggaggg cgcaaacata cgatgattac gtagcgacac tgcgcttcga cggcgcaccg
      901 gtgaggatct gagatgcaca tctttcccgt cagggtgtat tacagcgata ccgactgcgg
      961 tggaatcgtc taccacgccc ggtatctcga ttttgccgag catgcccgga ccgaactgct
     1021 tcgcgagatt gcacagcggc ataggttcga cggcaaccag tcggcgatgc tgaatctgtc
     1081 gaagaccggc ttcgtggtga aatcggtcac gatcgattat cggaagcccg ccaagctgga
     1141 tgacttgctt gaagtacgga cgacggtcac ttcaatgaag cggttttcga tgacgcttac
     1201 gcagcaggtc ttcaaggacg aggacctgat cgccgatctc ctggtaaaag tggcggcgat
     1261 ccacttggac acactcaagc cgcagccgtt gggtgatgat cttcaaaaag cgtttctggg
     1321 agggtgatgt ggcctttacc gtgtataagg gagcagcgga atcaagcctc ccgattgcgc
     1381 ttgttccgct ctggggggag tatccgaacg tcgaagggtc ggtgcggctc gggcttcggg
     1441 atacgacctt gctgctcatc tttaaaatgc gttctcccca acttctgcgc atggtggaca
     1501 gacacaatgg gcccgtgtac agggacagct gcgtggaagc gtttttacag caacagggaa
     1561 gggacgagta tctgaacttc gagttcagcg cctcggggag catccttgcc gccaaaggga
     1621 aaaaccgcga aaaccgaaca tttttcgacc ccgatctgat cgatacgatc cctcggacga
     1681 ttgaaatcct ggagaataac aaccgtcaaa gtcggtatgt cgtttccgtt gagctcgatc
     1741 tgagagcgtt cggtctcgtc gaagaagggc gagaggtgaa tcttcgggga aacttcaacg
     1801 cgtgcggcga cgggcacaag gttccctact atctggcggc aaacccgatc ggaacggtga
     1861 agccggattt tcacgtcccc gacttcttcg ctcccctgat catcaccggg cgttgaacac
     1921 ttccactccc gaaagctccg cttcaccaag gagcttcgtc agggcaaggt gggcgaggtg
     1981 ctggccgaag atcgcggtga cggtgggaag ggatccaagg acgttccgcc ttcttcccgt
     2041 ttgactgatt cgctcattga aatcggcgtg ctcttcttcg gacgggtctt tgtagatgaa
     2101 gcggatttct tccgctgaaa agattacatc gatcccccgg ccgacgcccc tgcgcttgag
     2161 gcggtaacgc acctgcttgg ccagcggaca cccccacgta tccatgagat ccgccttgcg
     2221 caccaggaag ggatcgcgcc gaagggcggc gcccatcgat gaaattgcag gaatgttcgc
     2281 cttgtacgca tactcgagca acgcgacctt cgggctgagc gaatcgatcg cgtcgatgac
     2341 cagatccgtc tctttgcctt caaagatccg atgaaccgac tccttgttca cgaaaagagg
     2401 aagtacttca acctcgcagg acggattgat cgcgccgatc cgctctttcg ccacctgcgt
     2461 cttttcgctc ccgatcgtat cgtaggtcgc aagaatctgc cggttgaggt tggtgatccc
     2521 gatcgtgtcg aaatccacga tgcgcacctt gccgaccccg ctgcggacga gcgactccaa
     2581 agccattcct ccgaccgctc cgagaccgac gacggtaacg gtcttatcgt gaagggtttt
     2641 gatcgccgag tcgccgagca gccggctaat gcgcaagaat tgttcgtttc tcatcgttgt
     2701 ttctcaccaa tcggtcaagg ctccagcccg tcaactcggc aaaccatctc tgatgagcct
     2761 gccaggtttc cggatatcca tcctccgtcg gcttttcata atcgctttca aggccgaacg
     2821 gaagccgggt gagccgcttt ccttcgccgc tcagctttga atgcttcaac ctcggtccgt
     2881 agctgaggat gatatccaga cgagcggctt cacgggcgac ctcatagctg ccgccgaagg
     2941 agtgccacag gaggcgggga agcctctcta tgccccttag aatctgaagc agccgccctt
     3001 ctcttcgaac gcagtgaagc gttacgctgc gctccagttc gacggcgacc ctcaagagtt
     3061 cttttagaaa cgattcctgc cgatcgggat ggggaaatct tcggtcgaga ccgacctctc
     3121 caatctggag atgcgggtgg gcgacgagaa gagcatgaag ctcttcaaca ccatccggca
     3181 gttgcgacgg caggagcccg atcgccccgt agggggcgtg gatcaccgtt tcccactcct
     3241 ctttcctgct tgtcgcatag aaacaggcat gtgatggaga agctgaagaa aggtggcggt
     3301 gcaggtcgaa catcctatgc tccaagcaag agtgcaagat atgttaccac gatcgaggag
     3361 agaatcggaa gaatcatccc gcctttgaag tatgcgagga tgaaggcggc ggaaattccg
     3421 gcaaaccccg agtagaggtg cggaacataa tcgaggaaca ccccgggaaa gatcagcggc
     3481 ccgaaggcgg cgatcggaag gaggcggagc atccgggaaa gcagcggcgg cagcttgtcc
     3541 agaaaggaaa cccaataggg gatgacccgg ggaatgaagg tcgcaagcgc ggagacgagg
     3601 atcggaacca cgaatgtttt catacatcct cccgaataat gagtgagccg atgaagcttg
     3661 caaggagcat cgagacgacg aacgaccaac cgatctttac tccaagaagc agcgtcaaaa
     3721 ccgagtgggt tcccgccgag atcgcggcga tgagaaggac ctttatgcct tttttacgaa
     3781 actcctgagc caacagcgat gagaacatcg catagatcgt cactccaacc gccagctgaa
     3841 gggcgggtgg gaggaccaag ccgacgagaa agccaagcgc tgttccgctg acccatccgg
     3901 cgtaggaggt cagctccaag cccgccatgt acgaggcggg aagccttccg ggattcatca
     3961 acgagatgga aaacacttca tcggtcgttc cgtgggcgat gagcgcccgc ttcatccccg
     4021 tgattccatc ctccagccgc cgggcgacct ccgctcccat gaaggtgtac cgaaggttga
     4081 tgagcaggac actgatgaac agttcggcaa ggaaggcgcc cgatccgatc aggctggctg
     4141 cgaggaattg gccgctgccg gcaaaattcg tgacggaaaa gagcaccgct tcccaaagac
     4201 gcaatcccag attctttacc acgaggccaa acgccatcgc ggtgggaaaa tacccgatga
     4261 agatcggaaa gccgtcttta cagcccgtag agagggtaag attgcgttcg ctcataaacg
     4321 tgagcgtagt aagttaaaac cgttttgtcc agtaatggca gtatggcagg gttccttttt
     4381 tccggtagta gtcctgatgg taggattccg cccgatagaa catcttttga ggaagcaccc
     4441 gggttgcaac cttcataccc cccgtttcga gccggcggat cagcttgagt gcagtgtcaa
     4501 actggtcgcg gctgcgataa aagatgacgg attcgtactg ctttccgata tccggtcctt
     4561 gcccatccac ctgaccggga tcatggatct ccaaaaaata cttggtcagc tcctcatagg
     4621 tgatcttcag cggatcgtac gtgaccttga cggtttcata atgaccggtc ttcccggtga
     4681 ggacctcttg gtaggtaggg ttttccgtcg ttccgccgct gtagccgctt tccgctgtcc
     4741 gcaccccgct tttcttggaa aagagatact gggttcccca gaagcaccca ccggcatata
     4801 aggcggtggt gaccggttcg cccggtctga agaccatgct gatcgaattc acgcagtggc
     4861 gggtgtttgt cggagtgaag ccttcacctt caaagacgtg cccgaggtgc gcaccgcagg
     4921 taaagcagat gatctcggt
//
LOCUS       DAOPUZ010000022         4845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_17574_length_4845_cov_3.277453, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000022 DAOPUZ010000000
VERSION     DAOPUZ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17574_length_4845_cov_3.277453"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PKN79_00825"
     CDS             complement(<1..803)
                     /locus_tag="PKN79_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADH-specific enoyl-ACP
                     reductase/trans-2-enoyl-CoA reductase"
                     /protein_id="HNZ94344.1"
                     /translation="MVITRKVLRNVSLTAHPLGCRRHVEDQIAWVKNPKGSLYSPAPN
                     RMPARVLVIGGSTGYGLSSRIVAAFGGGADTINVSFEREPGEKKTATPGWYNTKVFEE
                     HARKSNLRAFSVFGDAFSDEVKEETADLIEKEFGQVDLIVYSLASPVRIDPRTGATYR
                     SVLKPIGKPYSALSVNLDDPTITKETIEPATVEQTEETVKVMGGEDWKLWIDLLDRRN
                     LLAEGAVTVAFSYIGPEVTYPVYREGTIGKAKEDLEKTAGVLSKTLKAYV"
     gene            complement(805..1242)
                     /gene="fabZ"
                     /locus_tag="PKN79_00830"
     CDS             complement(805..1242)
                     /gene="fabZ"
                     /locus_tag="PKN79_00830"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNZ94345.1"
                     /translation="MNIVKLSPQEILPHRDPFLFVDEILEADESHTVAKRVFTPDHFF
                     FKGHFPDYPVVPGVLLIETMAQAGGAGLGQAGIIPLGSLFVLATIEKAKFRSQVRPGD
                     EAVITVENVRISQMVLRQKGTITVNGTVAAEASWMCILVKGEE"
     gene            complement(1239..2480)
                     /gene="fabF"
                     /locus_tag="PKN79_00835"
     CDS             complement(1239..2480)
                     /gene="fabF"
                     /locus_tag="PKN79_00835"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNZ94346.1"
                     /translation="MRRVVVTGMGTVNPLAHNVEEFWSRIKAGESGIGPLTKFDASAY
                     PAQIAGEVKEFDPSTLVDKRALRSMADFTKFAAYSAVQAMNQANLKEGSFDPHRSGVF
                     LGNGIGGFEVVEENLAKLFEGGPSRVAPLTIPKLISNEAAGNLAILFGFKGPAHTVVT
                     ACASGTDAIGEAFNRIRFGLVDVALTGGTEAAITQLAVAGFCRLQALTTKYNDDPKAS
                     SRPFDKDRSGFVMGEGAGILVLESLEHARKRGARILGEVVGYSMTCDAFHLTAPDPEG
                     SGAVRAMANALSMSGAKIDEIDYINAHGTSTQANDIMETNAIKSVFGEHAYRLSVSST
                     KSMTSHLIAAAGAVEAIICLLALRDQYLPPTINLDEPDEGMDLDYVPNHGRSGRIRYA
                     MSNSLGFGGHNGVLVFTTYKE"
     gene            complement(2491..3204)
                     /gene="fabG"
                     /locus_tag="PKN79_00840"
     CDS             complement(2491..3204)
                     /gene="fabG"
                     /locus_tag="PKN79_00840"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNZ94347.1"
                     /translation="MNTKKHALVTGGSRGIGSAIIRELLSLHYEVWYLSRTKNEELDN
                     ESLHHIRCDIADREEVERALKAIGDEARGIDLLVNNAGITRDGLIMRMKDEAWDDVLA
                     VNLSSVFLTCRAVSRIMARQRSGAIVNISSVVGIMGNGGQTNYAASKAGIIGFSKALA
                     RELAARNVRVNVVAPGFIDTSMTKTLAEEAKELIKTQIPLGRMGMASEVAKAVAFLAS
                     EDASYITGAVLPVDGGMAI"
     gene            complement(3201..4139)
                     /locus_tag="PKN79_00845"
     CDS             complement(3201..4139)
                     /locus_tag="PKN79_00845"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNZ94348.1"
                     /translation="MAPIIALYPGQGSQEPKMALDLHQASPRVRELFTLAGDITHVDL
                     RNLLENGGREELQRTKNTQLAVTLASRSAHVRLTELGHVFTTHSGFSLGELSAYAGSG
                     IISDETLFELVAIRAALMEQEAQRIQAALGEVGMAAVIGLPYDAVSELIKDRNDVYAA
                     NDNSPVQVVISGTLKAIEEVQERLKEHGARRIIPLRVSGPFHTPLMEGAIPCFEAAIG
                     SLPFHDPRELVISSVTGMPVTSATQAKRTLITQLSKPVRWTKAVNTITSVKDSDSIVG
                     EVGHGEILAKLVQDRLSVLPLGSEERIRAFHKGIKQ"
     gene            complement(4124..>4845)
                     /locus_tag="PKN79_00850"
     CDS             complement(4124..>4845)
                     /locus_tag="PKN79_00850"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HNZ94349.1"
                     /translation="GVIVATATGDYSGFPSTANLVSRHFKTSGGAFDVAAGCTGFIYA
                     LDVARSMILSGTLKNALVIGSETLSTVIDWKDRNTCVLFGDGAAAILLEQVASGGLSP
                     ALLKSESEQAGSLTIDPASRHITMDGKAVYQFAVRAIGKIITDVLKANNLSISEIDWI
                     VPHQANRRIISATSKRYGIPEELFYLNIERVANTSAASIPLALSEMETGCLLKEGQKI
                     LLVGFGAGLTYGGSLVTWHRS"
BASE COUNT          856 a         1458 c         1476 g         1055 t
ORIGIN      
        1 acatacgcct taagggtctt ggaaagcacc ccggcggtct tttccaggtc ttccttcgct
       61 ttcccgatcg tcccttcccg gtagacgggg taggtcacct ccggcccgat gtaggagaag
      121 gcgacggtga ccgccccttc ggcaaggagg tttcttctgt caagaagatc gatccacagc
      181 ttccagtcct cgccccccat caccttgacc gtctcttccg tctgctcgac cgtggcgggc
      241 tcgatcgtct ccttcgtgat cgtggggtcg tccaggttca ccgacaaggc ggagtagggc
      301 ttaccgatcg gttttaagac cgagcggtag gtcgccccgg tcctagggtc gattcggaca
      361 ggacttgcca gcgagtagac gatgaggtcg acctgtccga actccttctc gatgagatcc
      421 gccgtctcct ccttcacttc gtcgctgaac gcatcgccga agacgctgaa cgccctcaga
      481 ttgctttttc gggcgtgctc ttcaaacacc ttggtgttgt accaacccgg ggtggcggtc
      541 ttcttctcgc ccggttcccg ctcgaaggag acgttgatcg tatccgcccc gcctccgaag
      601 gcggcaacga tccgggagga gaggccgtag ccggtggacc ccccgatgac gagcacccgg
      661 gcgggcatcc tgttcggagc gggggagtac aaagatcctt tcgggttttt gacccaggcg
      721 atctgatcct cgacatggcg gcggcacccc aaggggtggg cggtcagcga gacgtttctc
      781 agcacttttc tggtgatgac catctcattc ctctcctttg acgaggatgc acatccagga
      841 cgcctcggcg gcgacggttc cgttgaccgt gatcgtcccc ttctggcgaa gaaccatctg
      901 agagatccgg acgttttcga cggtgatgac cgcctcgtcc cccggcctta cctgcgagcg
      961 gaacttcgcc ttttcgattg tggcgaggac gaagagggag cccagaggaa tgatccccgc
     1021 ttggcccagc cccgctccgc ccgcttgagc catcgtctcg atgaggagca ctccggggac
     1081 gaccgggtag tcggggaaat gccctttgaa gaagaaatga tcgggggtga agacccgctt
     1141 tgccaccgtg tgggactcat ccgcttcgag gatctcgtcg acgaagagga acggatcgcg
     1201 gtggggcaat atctcttgag gactcagttt cactatattc actccttgta tgtggtaaag
     1261 acaagcacgc cgttgtggcc tccgaagccg agtgagttgc tcatcgcata gcgtatccta
     1321 ccacttcggc catggttcgg tacatagtcc aaatccattc cctcatccgg ctcgtcgagg
     1381 ttgatcgtcg gcggaaggta ctggtcgcgc agggcgagga ggcagatgat cgcttccacc
     1441 gccccggcgg ccgcgatgag gtggctggtc atgctcttcg tcgatgagac gctcaacctg
     1501 taggcatgct cgccgaagac cgatttgatc gcattggtct ccatgatgtc gttcgcctgc
     1561 gtcgaggtcc cgtgggcgtt gatgtagtcg atctcatcga ttttcgctcc gctcatcgaa
     1621 agcgcgttcg ccatcgcccg aaccgccccg gagccctcgg gatcgggagc ggtgaggtgg
     1681 aacgcgtcac aggtcatcga atacccgacc acttcaccca gaatccgagc tccccgcttc
     1741 cgggcgtgct ccagcgactc gagaacgaga atgcccgctc cttcacccat tacgaagccg
     1801 ctccgatcct tgtcgaacgg cctgcttgat gccttggggt cgtcgttgta cttggtggtg
     1861 agggcctgga ggcggcagaa cccggccacc gcgagttggg tgatcgccgc ttccgtgccc
     1921 ccggtgaggg cgacgtctac caagccgaag cggatccggt tgaacgcttc cccgatcgca
     1981 tcggtgccgg aggcgcaggc ggttacgacc gtatgggcgg ggcctttgaa gccgaagagg
     2041 atcgccaagt ttcccgccgc ctcattgctg ataagcttgg gaatcgtcaa gggggcgact
     2101 cgggagggtc ctccttcaaa gagcttggcg aggttctctt cgacgacctc gaagccgccg
     2161 atcccgttgc ctaagaagac gccgcttcga tgggggtcga acgacccttc tttcaggttt
     2221 gcctgattca tcgcctgaac cgccgaatag gcggcgaact tcgtgaaatc cgccatcgag
     2281 cggagtgccc gcttgtccac cagcgttgaa ggatcgaact ccttcacttc tccggcaatc
     2341 tgcgcagggt aggcggaggc atcgaacttg gtgagggggc cgatcccgct ttctcccgcc
     2401 ttgatcctgc tccagaactc ttcgacgttg tgggccagtg ggttcaccgt tcccattccg
     2461 gttactacga ctcgtctcat cgcttcctcc tcagatcgcc atcccgccgt cgaccggcag
     2521 gaccgccccg gtgatgtagg aggcatcctc cgacgccagg aatgcgacgg ctttggcgac
     2581 ttcgcttgcc atccccatcc ggcccaaggg aatttgggtt ttgatcagct ctttggcctc
     2641 ctcggcaagg gtcttcgtca tcgaggtgtc gatgaacccg ggggccacca cgttgacccg
     2701 gacgttccgg gcggcgagct cccgggccaa agccttggag aacccgatga tcccggcctt
     2761 cgaggcggcg tagttggtct ggccgccgtt tcccatgatg cccaccacgc tggagatgtt
     2821 gacgatcgcc ccggagcgct gtctggccat gatccggctc accgcccgac aggtgagaaa
     2881 gacgctgctc aagttcacag cgagcacatc gtcccacgct tcatctttca tccgcatgat
     2941 gaggccgtcc cgggtgatgc cggcgttgtt gacaagcagg tcgatcccac gggcttcatc
     3001 gccgatcgct ttgagagcac gttccacctc ttcccggtcg gcgatgtcgc agcggatgtg
     3061 gtggagactt tcattgtcca attcttcgtt cttcgtgcgg ctgagatacc acacttcgta
     3121 gtgtagactg aggagttccc ggatgatcgc cgatccgatt ccccgcgaac cccccgttac
     3181 gagggcatgc tttttcgtgt tcattgcttg attcctttat gaaatgcccg gatccgctct
     3241 tcgcttccca gggggagaac ggagagccgg tcctgaacga gcttggcgag gatctctcca
     3301 tggccgacct cgccaacgat cgagtccgaa tcttttactg aggtgatggt gttcaccgcc
     3361 tttgtccacc tgaccggctt cgagagctgc gtgatgagcg ttctttttgc ctgagttgca
     3421 ctggtgacgg gcatgccggt gacggaggag atgacgagtt cccggggatc atgaaacggc
     3481 aggcttccga tcgccgcttc gaagcagggg atcgctcctt ccataagcgg ggtgtggaag
     3541 gggccggaga cccgaagcgg gatgattcgt ctcgctccat gctctttcaa acgttcttga
     3601 acctcttcga tcgccttcaa cgtacctgaa atgacgacct gaacgggcga gttgtcgttt
     3661 gcggcgtata catcgttgcg gtctttgatg agctcggaaa cggcatcata cggaaggccg
     3721 atgacggcag ccatgcccac ctccccgaga gccgcttgga ttctttgggc ttcctgttcc
     3781 atcagagcgg cgcggattgc aacaagttcg aagagcgttt cgtcactgat gatcccgctt
     3841 cccgcatagg cggaaagctc cccgagactg aagccgctgt gggtcgtaaa cacatggccg
     3901 agttcggtca ggcggacatg ggcgctccgg ctggcgaggg ttaccgcaag ctgggtgttt
     3961 ttggtccgtt gaagctcctc cctgccgccg ttctccagaa gattccgaag atccacatgg
     4021 gtgatgtcgc cggcgagggt gaagagctcc ctgacccggg ggctggcttg gtggagatcg
     4081 agggccatct tcggctcctg agagccttgg ccggggtaga gggctatgat cggtgccatg
     4141 tgacgagact ccctccgtag gtcaggcccg ctccgaagcc gacgagcaga atcttctgcc
     4201 cttccttcaa caggcagcct gtctccatct cggaaagggc aaggggaatc gaggcggcgc
     4261 tcgtgtttgc gacgcgctcg atgttcagat aaaagagttc ttcggggatt ccgtagcgct
     4321 tgctcgtcgc actgatgatc ctccggttcg cctgatgggg aacgatccaa tcgatctcgg
     4381 agatcgaaag gttgttggct ttcagcacgt cggtgatgat ctttccgatc gctcggacgg
     4441 cgaactgata taccgccttc ccgtccatcg tgatatgccg ggaggcgggg tcgatcgtga
     4501 ggctgcccgc ctgctcggat tcgcttttca gcagggcggg ggagaggccg ccggaagcga
     4561 cctgctcaag gagaatcgcc gcagcaccgt cgccaaagag gacgcacgtg tttcgatctt
     4621 tccaatcgat cacggtggag agggtttccg agccgatcac gagggcgttc ttcagcgttc
     4681 ctgaaaggat catcgaccgg gcgacatcaa gggcgtagat gaagccggtg caccccgccg
     4741 ccacgtcgaa cgctcctccg ctcgttttga agtgccgact gacgaggttc gccgtggagg
     4801 ggaagccgga gtaatcgccc gtcgcggtgg cgacgatgac gccgt
//
LOCUS       DAOPUZ010000023         4764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_18003_length_4764_cov_2.557868, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000023 DAOPUZ010000000
VERSION     DAOPUZ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18003_length_4764_cov_2.557868"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1345
                     /locus_tag="PKN79_00855"
     CDS             113..1345
                     /locus_tag="PKN79_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNZ94350.1"
                     /translation="MTFALISCTSVKTFEKRALKIIPDPIAYLVDRGLPAYPSSDITL
                     FNTGREWNVHTLKRIADAKEYILVTVFLGNIHEVSYEVWDALAEKVKEGVDVYVLLDS
                     SSYFQLTPHTNLVVPAAFNYARDIGLKVAEYNSMSVSHAAFLPLLLDRDHRKIWIFDG
                     EYLAVGGINLNEASLMIPPETGNIDTMVEIQSPQAIEALVDSFVRTWERYSVQPIDRT
                     AFSVPPKENLPKTVYLSDHYFFADNTVTDLFDALILGAQKEVWMIQGYSFLTKELINR
                     IKEATNRGVKVNIVLSENAVKVHYEKAAFFGMVDLIDAGATLYLFDGPHHAFLHCKLI
                     VADEKYSLFGSVNYNLRSQFLSREISFLFDDEAIAEKALEHVGFIIDHSRIVDREEAK
                     THRRIDYLFYNLLMQFIG"
     gene            1349..1822
                     /locus_tag="PKN79_00860"
     CDS             1349..1822
                     /locus_tag="PKN79_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94351.1"
                     /translation="MKRLFLILLFLLLGTSVLLSAPWLGGYRLSFDGYQDRPGQYYGA
                     HLYLEPLWGTTIGLGFSVGGATSFGLSLPSSLFESSVEIRLLNVEHRLFRRPSTWRIA
                     LSGGIVTDFETGHWYAQLDPFVFFFGEKTIKVLGLRLLDDRSWALRLLEITHYFF"
     gene            1831..2328
                     /locus_tag="PKN79_00865"
     CDS             1831..2328
                     /locus_tag="PKN79_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94352.1"
                     /translation="MKRTIGVVLIILINLSLFALPVALSFETWNSKAIAGPAASAGVS
                     VGVLPNLELQGAFLAELTPRIGNRIGAFGALTIGLVNPHHYRDPARAPLYYNALVGVG
                     YLGGWNMAEGKPFGEIFVRLTPLSLGSSYYRTRERTFGIGVGFDHYTHRWSFIVNMFA
                     FDRYL"
     gene            2522..3283
                     /locus_tag="PKN79_00870"
     CDS             2522..3283
                     /locus_tag="PKN79_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94353.1"
                     /translation="MVDVLAITLSPSESFSIAVTGLEAYCLPGEDTITKAALTYQTYR
                     PLPFALHVHCTKRIPVQAGLGGGSSDGAAILLFLQELAGENKLGDEELLTAAKHVGSD
                     LPFFVSGAKAAIVEGTGEVVVPLPCVSYPALVVKPVNLNISTKNAYGSLDRIMDRPLQ
                     WADRGDIASAFKDSIPNWHKTFYNDFEQTLSDLPFYRELRKLTARFDGYQGLSGSGPS
                     WFFIAESDQKVDVLAQMIDDRFGSHVAIYRLRVCS"
     gene            3314..3568
                     /gene="spoVG"
                     /locus_tag="PKN79_00875"
     CDS             3314..3568
                     /gene="spoVG"
                     /locus_tag="PKN79_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HNZ94354.1"
                     /translation="MEISEVRVRKVQQGGKLKAYVTVTFDDQFVVHNIKIIEGQEGNF
                     IAMPSRQLANGEFKDIVHPITSSFREELQNAIMKAYEEAD"
     gene            3615..3686
                     /locus_tag="PKN79_00880"
     tRNA            3615..3686
                     /locus_tag="PKN79_00880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3648..3650,aa:Gln,seq:ttg)
     gene            3844..4479
                     /locus_tag="PKN79_00885"
     CDS             3844..4479
                     /locus_tag="PKN79_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNZ94355.1"
                     /translation="MGNKRTLSASLRTEDFGSRGARRLLRSNRIPAVIYGKNDPVHIS
                     LDNQEFSNKVRHFSETALLKVMVGENEYECLMKDYQEDLIRGQIKHVDFYEVTSGQLL
                     RTMVSLVLHGTPIGVREGGVLDQVAYEAEIESLPSDLPENIQIDVSGLELNGVLTLED
                     VEFPKGVKLLSDPEGVVAIVQSIREEVEEEEEEDADEPVLVGAKKGSDEED"
     gene            4479..>4764
                     /locus_tag="PKN79_00890"
     CDS             4479..>4764
                     /locus_tag="PKN79_00890"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNZ94356.1"
                     /translation="MMIILGLGNPGSKYENTRHNAGFNVTDRLAAFFHVKLRKRCFRP
                     YRQARIEDSMIVQPLTYMNQSGSVLKYLPEEKELIVVCDQLDLPPGMIRIR"
BASE COUNT         1105 a         1166 c         1271 g         1222 t
ORIGIN      
        1 cgcaattgga tgaactggga gtctttcacg agtagaactc ttgtcaccat gcaagagaga
       61 tatggtatag tcgctacata tgaggagaac gaccgctctc atagtcttgg caatgacctt
      121 cgcgctgatc tcgtgcacca gcgtgaaaac ctttgagaaa cgagccctga aaatcatacc
      181 cgacccgatt gcgtaccttg ttgatcgcgg tctgcctgcc tatccttcaa gtgacatcac
      241 cctcttcaat accggccggg agtggaatgt ccacaccctg aaacggatcg ccgatgcgaa
      301 agagtacatc ttggttacgg tttttcttgg caacattcat gaagtaagtt acgaagtatg
      361 ggatgcgttg gccgagaagg tgaaagaagg ggttgatgtg tacgtgttgc ttgactcctc
      421 ctcctatttt caattgactc cgcataccaa cctggtggtt cccgctgcgt tcaactatgc
      481 ccgggatatc ggtttgaagg ttgccgaata taattcaatg agcgtaagcc atgcggcgtt
      541 ccttccgctg ctcctcgacc gggatcatcg gaagatctgg atttttgatg gcgagtatct
      601 ggccgtcggc ggcatcaacc tcaatgaagc atcgttgatg attcctccgg aaacggggaa
      661 catcgatacg atggtggaga tccaaagccc gcaagcgatc gaggcgctcg tcgattcctt
      721 cgttcggaca tgggaacgct acagtgttca accgatcgac cgcactgcgt tttccgttcc
      781 tccgaaagag aatctgccga aaaccgtcta tctgagcgac cattattttt tcgctgacaa
      841 taccgtaacc gatctcttcg atgccctcat cctgggagcg caaaaagagg tgtggatgat
      901 tcaaggctac agcttcctga ccaaggaatt gatcaaccgg ataaaagaag ccaccaaccg
      961 gggagtgaag gtgaatatcg tgctcagtga gaacgctgtg aaagttcact atgaaaaagc
     1021 cgcgttcttc ggaatggtcg atctgatcga cgcaggagcc acgctctatc tcttcgatgg
     1081 gccgcatcac gcgtttcttc actgcaagct gatcgttgcc gatgaaaagt attcgctttt
     1141 cggatcggta aactataatc ttcgttccca atttctttca agggagatca gcttcctctt
     1201 tgatgatgaa gcgatcgccg aaaaggccct tgagcatgtg ggattcatca tcgaccacag
     1261 ccggatcgtc gaccgggaag aagcaaaaac gcatcggagg atcgattatc tcttctataa
     1321 tctcctgatg cagttcatag ggtagggtat gaaacggcta ttcctgattt tactgtttct
     1381 tctccttggg acaagcgtac ttctaagcgc tccctggctc ggcggctatc gcttgagctt
     1441 tgacgggtat caagacaggc cggggcaata ctatggcgct cacctctacc ttgagccgct
     1501 ttggggaaca accatcggtt tgggcttcag cgtcgggggg gcgacgagct tcggtctctc
     1561 tcttccttct tccctctttg agagttcggt tgaaatccgt ctcctcaatg ttgagcacag
     1621 gctgttccgg cgcccctcga cgtggcgaat cgccttaagc ggagggatcg ttaccgactt
     1681 tgaaaccggc cattggtatg ctcaattgga tccgttcgtg tttttctttg gagaaaagac
     1741 gatcaaggtg ttggggcttc gcctccttga cgatcgctcg tgggcgctgc gcctgttgga
     1801 aatcacccat tatttctttt aggaggcggt gtgaaacgaa caatcggtgt tgtgctcatc
     1861 atcctgataa acttgagcct ctttgcactg cccgtcgccc tttcgttcga gacgtggaac
     1921 tccaaagcga tcgccggtcc ggcggcaagt gcgggagtca gtgtgggagt ccttccgaat
     1981 cttgagcttc aaggagcgtt tttggcggag ctgaccccac ggatcggaaa ccggatcggg
     2041 gctttcggag cgctcaccat cggtctggtg aatccgcatc actatcgtga tccggccaga
     2101 gctccgctct attacaacgc gctcgtggga gtggggtatc tcggtggatg gaacatggcg
     2161 gaaggaaagc cgttcgggga gatctttgtc cgactgacgc cgctttcatt gggttcatcg
     2221 tactatcgca cccgagaacg aacgttcggt attggcgtcg gttttgacca ttacacccat
     2281 cgttggtcct ttatcgtgaa catgtttgcc tttgaccgtt acttatagat gccaatatgc
     2341 accatcggca aggaaatttt atgtcttgta ctggacagtt ccaccatgct ttgctactct
     2401 gggcggtatg gaaacgcaaa tcgtccgatt tgctccggcg aagctcaatc tccatttggc
     2461 ggtcgggcct ctcgagcaga atggactcca ttcaattcag tctctctttg tgactgcttc
     2521 tttggttgat gttctcgcga taaccctttc accgagtgaa agcttctcca tcgccgtaac
     2581 cggcctcgaa gcgtactgcc tgcccggaga ggacacgatc accaaagctg ctttgaccta
     2641 tcaaacatac cgcccgcttc cctttgctct gcatgttcac tgcaccaagc ggattcccgt
     2701 tcaagccggg cttggcggtg gctccagtga cggagcggcg atcctgctgt tcctccaaga
     2761 acttgccggg gagaacaagc tcggtgatga agagcttctc acggcggcaa aacacgttgg
     2821 cagcgatctt cctttctttg tcagcggagc gaaggcggcg atcgttgaag gcactggaga
     2881 agtggtcgtt cctcttccct gcgtttcata tcccgcattg gtggtaaaac cggtcaatct
     2941 gaatatatcg acgaaaaacg cctatggttc cctggatcga atcatggatc gaccccttca
     3001 atgggcagat cgcggagata tcgcttcagc atttaaagat tcaataccaa attggcataa
     3061 aacgttttat aatgatttcg agcagacact ttccgatctt cctttttaca gggaattgag
     3121 gaaacttacg gcaaggtttg acggctacca agggttgagt ggaagtggac catcttggtt
     3181 tttcattgca gaatccgatc aaaaagtcga tgtacttgca caaatgatcg atgatcggtt
     3241 cggttctcac gtagccatct atcgcctgag ggtgtgctcg taggtgattt gatacagtcc
     3301 cgcggaggtt agtgtggaga tttcagaagt tcgagttcgc aaagtgcagc aaggtggcaa
     3361 gctgaaggca tacgtaacgg ttaccttcga tgaccaattc gtggttcaca acatcaagat
     3421 cattgaaggt caggagggca atttcattgc gatgccctcc cggcagctgg ccaacgggga
     3481 gtttaaagat atcgttcatc caatcaccag cagcttccgc gaagagctgc agaatgcgat
     3541 catgaaggcg tacgaggaag cggattaacc ttactggaca aaacggtttt tttacgatag
     3601 ggtctaaccg ttattgggaa gtggccaagt ggttaaggct ctggtttttg gtgccggcac
     3661 cgggggttcg agtccctcct tcccagtttg gggcaatcct gtggatcgat tgcccttttc
     3721 ttttacgagg gccttgtata gaagggaata agttttctat actcggttct tggcgatatg
     3781 tgttgcatat cctctaatgt catggtaatt ggcaatcggc caatgagagt acaggagtaa
     3841 acaatgggta ataagcgaac actgagtgcc tcactcagaa cggaagattt cggaagtcgc
     3901 ggagcacgaa gactcctccg aagcaatcga atcccggcag tcatttatgg aaagaatgat
     3961 cccgtccata tcagcttgga caatcaggag ttcagcaaca aagtacggca tttttcggaa
     4021 acggcgctcc tgaaggtcat ggtaggggag aacgagtacg aatgcttgat gaaggattat
     4081 caggaagatc ttatccgcgg tcagatcaag cacgtagatt tttatgaagt tacctccggt
     4141 cagcttcttc ggacgatggt cagccttgtg ctgcacggaa ccccgatcgg tgtccgtgaa
     4201 ggcggagttc tcgatcaggt cgcctatgag gcggaaatcg agtcattgcc ctccgacctg
     4261 cccgagaaca tccagatcga tgtttccggt cttgagctca atggtgtatt gacccttgaa
     4321 gatgttgagt tccccaaggg cgtcaagctc ctcagcgatc ccgaaggggt tgtcgcaatc
     4381 gtccagtcga ttcgggaaga ggttgaggaa gaagaggaag aggatgccga tgaacctgtt
     4441 cttgtcggtg ccaaaaaagg gtctgacgag gaagattaat gatgattatt cttgggttgg
     4501 ggaatcccgg atccaagtac gagaatactc gccacaatgc agggttcaat gtaaccgacc
     4561 gtttagcagc gttttttcac gtgaagttga gaaaacgctg ttttcgtccc taccgacaag
     4621 cccgaatcga ggattcgatg atcgttcagc cgctcaccta tatgaatcaa agcggttccg
     4681 tgctcaaata tctccctgag gagaaggaat tgatcgtcgt ctgcgaccag ctcgatctgc
     4741 cgccggggat gattcggatc cggc
//
LOCUS       DAOPUZ010000024         4752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_18072_length_4752_cov_2.933362, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000024 DAOPUZ010000000
VERSION     DAOPUZ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18072_length_4752_cov_2.933362"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..699
                     /locus_tag="PKN79_00895"
                     /pseudo
     CDS             59..699
                     /locus_tag="PKN79_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
     gene            complement(683..1912)
                     /locus_tag="PKN79_00900"
     CDS             complement(683..1912)
                     /locus_tag="PKN79_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNZ94357.1"
                     /translation="MTEQDIRRYAELIITKGINLQKGKAVLITTGVGTYYFARALGKA
                     AYRLGASYVQILTDDLDLLSSRLEAQSEEEVQFVPAYLKALDYEFISEGWSFIRVDST
                     EERLDHGPLDPEKNRLASAARRKASEARSRVLMRHQLPWCVCVAPGPLWAKQVLGEDA
                     KPEDLFKVLKPILLLDREDPGTAWDEKHETLQKRMDFINALDFKALHFQSPRTDLTIG
                     FRKEARFVGGAETLPSGKRFFANLPTEEIFSTPDRMQVEGYVTTTRPVEVLNTPTEEV
                     RLVFKEGKVVEHSAKKGQEVLDRFFEIDEGARRIGEVALVDESSPIAQSGLIFHSILL
                     DENASCHLALGEGYPICLEHGSTLSSEEELHEAGCNTSLVHVDFMVGSSDMNVDAILS
                     DGSRVPIIVDGRFSLNH"
     gene            complement(1927..3096)
                     /locus_tag="PKN79_00905"
     CDS             complement(1927..3096)
                     /locus_tag="PKN79_00905"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNZ94358.1"
                     /translation="MQERYAELVIAVQLRLIEGDSLTINTNARTMSFARLVARKATEV
                     TKLPVTIVETSMGKVLQTYPIDPVEKGVMRPQVRTSVLAHIVDLDEFPYDEDEDWNEP
                     HHEVRTLAHYGHLSDPVFLDRRISIPWANIPYPETAWAASMLGRLVSREEMWSLFGIL
                     MRLTSDWSPAYWAEQANVLSYREKLLNASSGRMISIKGSNTELIGEQSPLTGWISGVF
                     RLPSGREYIPLLPVQSVHTAFKTGSVNGKIRSGSPFTLFGTPVTDAAFIIENGVVVSY
                     DAREGKEALKKYFDVDAGTNAASGISLADSNTLECRYITRLVHPHYKKECRSILHLGG
                     VLVDAIKAGTGEDDLFGLGQSIAHLEIPLAHDITVEMQKPDGSMKTIIAEGSFTV"
     gene            complement(3148..3218)
                     /locus_tag="PKN79_00910"
     tRNA            complement(3148..3218)
                     /locus_tag="PKN79_00910"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3184..3186),aa:Gly,seq:tcc)
     gene            3969..4205
                     /locus_tag="PKN79_00915"
     CDS             3969..4205
                     /locus_tag="PKN79_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNZ94359.1"
                     /translation="MGQRGEVFSTRLVKNERTYFFNVKENIRGDVFLNIVESKGLPDS
                     DRFIRQSIIIYQEDLGEFLKELQKSLDFIKQNTR"
     gene            complement(4202..4747)
                     /locus_tag="PKN79_00920"
     CDS             complement(4202..4747)
                     /locus_tag="PKN79_00920"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNZ94360.1"
                     /translation="MICGVDEAGRGPLAGPVVAAAVILGKHFPAHLLKDSKKLAPKQR
                     TMLETLIKEQAAGWTVAIVSHRRIDEINILQATLEAMHNAVSQLQKNHPITKALIDGN
                     RSPILSCETETIVKGDDSIPEIMAASILAKVERDRIMVAYDEQWPMYRFAEHKGYPTK
                     AHKEALKQYGPSPIHRLSFRY"
BASE COUNT         1061 a         1238 c         1291 g         1162 t
ORIGIN      
        1 gtggacggcc cactggcgga ttcagaccga tacggaaaag accgccgacg agtatgaggt
       61 gatattcaga acgctgatat ccaacggaca ggttcttccg ggggagatca cctcggtggt
      121 tctgagcagt gtggttccca cgctggtgca gccctttatc gacatgttgc agcgtttttt
      181 gaatctgcgg ccggtaatcc tgggccctga aacctacgac cggctgcccc tgggcatcct
      241 gaatccttat cagatcgggt ccgatctggt ggccaacgct ctggcggcct ggaccaaatt
      301 cggggaatat gtgatggtca tcgatttcgg aactgcactg acgttttaac ggtgaacggg
      361 gagggagcgg ttctcggggt ctcgatcgct cccggattgg taacagcggt gaactccctc
      421 ttcggagcga ccgctcaact gcctcaggtc gaattgaaaa ccccgccttc ggtgatcggg
      481 cgaaccagcc aggattcgat caacggcggc atcatgttcg gatatgcggg gttggtgaaa
      541 tcgatcatcg agctgacgga agcggagatc ggcgaagagc tctatgtgat cgccaccggg
      601 ggtctttcgc gaacgctcag ccccctggtt ggacgaatca atcagattgc gccgatgcac
      661 acccttgacg ggttgaaact gatcagtgat ttaaactgaa gcggccatcg acgatgatcg
      721 ggactctcga cccgtcggat aggatcgcat cgacgttcat gtcgctcgag cccaccatga
      781 agtcgacgtg gaccaggctg gtgttgcacc ccgcttcgtg aagctcttcc tcgcttgaaa
      841 gcgtggaacc gtgttccaga cagatcggat atccttcccc gagggcaagg tggcaggaag
      901 cgttctcatc aagcaggatc gagtggaaga tcaagccgct ttgggcgatc gggctgcttt
      961 catcgaccag cgccacttcg ccgatcctcc tcgcgccctc gtcgatctcg aagaaacggt
     1021 ccaacacttc ctgacccttc ttcgccgaat gctcgacaac ctttccttcc ttgaagacaa
     1081 gacgaacctc ctcggtggga gtgttcagca cctccaccgg gcgggtcgtc gtcacatacc
     1141 cttcgacctg catccgatcg ggagtggaaa agatctcctc ggtgggaagg ttcgcgaaga
     1201 agcgcttgcc ggagggaagg gtctccgctc ccccgacgaa tcgggcttcc tttctgaacc
     1261 cgatcgtcag gtcggttcgg gggctttgga agtggagcgc cttgaaatcc aacgcgttga
     1321 taaagtccat ccgtttctgc agcgtctcgt gtttttcatc ccaagccgtt ccgggatctt
     1381 ccctgtccag caaaagaatc ggcttaagca ccttaaaaag atcctcaggc ttggcatcct
     1441 cgccaagaac ctgcttcgcc cagagcggtc ccggggcgac acagacgcac cagggcagct
     1501 gatggcgcat cagaaccctc gagcgggctt cggaagcctt tcggcgggcg gcggaagcga
     1561 gccgattctt ctcagggtcg agcgggccgt gatccaatcg ctcctcggtg gaatcgacgc
     1621 gaatgaagga ccacccttcg ctgatgaatt cgtaatcaag cgccttgagg taggcgggaa
     1681 cgaattggac ctcttcttcg ctttgggctt ccaagcggga agagagcaga tcaagatcgt
     1741 cggtgagaat ctgcacgtag gaagccccca ggcggtacgc ggccttgccg agggcgcggg
     1801 cgaaatagta ggttcccact ccggtggtga tgagcaccgc cttgcctttc tgcaggttga
     1861 tgcccttggt gatgatcaat tccgcatatc gtcggatatc ctgttcagtc atcgttgtat
     1921 ctcctcctat acggtgaaag atccttcggc aatgattgtc ttcattgagc cgtcgggctt
     1981 ttgcatctct actgtaatat catgggcgag ggggatttcc aagtgggcga ttgattgtcc
     2041 aaggccaaac aggtcatcct ccccggttcc cgctttgatc gcatccacca gcacgcctcc
     2101 gagatgaagg atcgagcggc actctttttt gtaatgggga tggacgaggc gggtgatata
     2161 gcggcactcc agggtgttgg aatcggcaag actgattccg cttgcagcgt tggttcccgc
     2221 gtccacgtca aaatacttct tgagcgcttc tttgccttcc cgggcgtcat aggacaccac
     2281 aactccgttt tcaatgatga aggcggcatc agtcaccggt gtgccgaaca gggtaaacgg
     2341 cgatcccgag cggatctttc cattcaccga accggttttg aacgccgtat gaaccgactg
     2401 gaccggaaga agcggaatat actcgcgccc cgaggggagg cggaacaccc cgctgatcca
     2461 tccggtcagg gggctttgtt cgccgatgag ctccgtgttg cttcctttga tcgagatcat
     2521 ccttcctgaa gaggcgttga gaagcttttc ccgataagag agcacgtttg cctgctccgc
     2581 ccagtaggcg ggcgaccaat cggaggtaag gcgcatcaga attccaaaca ggctccacat
     2641 ctcttcgcgg ctcaccaggc ggccgagcat gcttgccgcc caggcggttt ccgggtaggg
     2701 gatgttcgcc cacggaatcg atattcttcg atccagaaag accggatcgg aaagatggcc
     2761 gtagtgggcg agggttcgca cttcatgatg cggctcgttc caatcctcgt cttcatcgta
     2821 ggggaattca tccagatcga cgatatgggc aagaacggag gtacgtacct gtggtcgcat
     2881 gacccctttt tcaacagggt cgatcggata ggtctgaagc actttgccca ttgatgtctc
     2941 gacaatcgtt acaggcagtt tggtcacttc agtcgctttt cttgcgacaa gccgggcgaa
     3001 gctcatcgtc cgggcgttgg tgttgatcgt aaggctgtcg ccttcaatca agcgaagttg
     3061 aacggcgatg accagttcag cgtagcgttc ctgcatgagc actccttacg aggacacaaa
     3121 aggcacccca tcggagtgcc tctctagagc gccctacggg agtcgaaccc gcttcttcag
     3181 cttggaaggc tgaggtaata ccattatacg aaaggcgcat caaccgcctc tttttatagt
     3241 gtatcgtttc atcttcgtca agtaccttga ggattccatc attcaaggct gtaagccaag
     3301 gctttaggcc cttgatataa tagactatca atggtatatt acaggtggtc atgatctatc
     3361 gatcgtgaat atacagaggt ctgccgctga accagcctat gctaatcagc ggtattttta
     3421 ttttatcatc gaagcatccc attttggatt ctgtagaatt ttttgataag ttctgggatt
     3481 ggccggatag catgtgatga attctccttc aagctctcct gtctttttat tccttcttat
     3541 cagtacaact acaacaaacg aatcaggagg atgaaatgca acgcgctttg aagctgagtg
     3601 cttcctcgtt ttataatccc agccacaacg gaattctgag gatggagatt gaagtattga
     3661 aagaacatca ggcattctac tagcacgagc aagggaaaaa gtatctttat ttgaccggtt
     3721 tgactcaaaa aatgcatgat aaaaatgatt ttttctaaaa cggattctta ttccgtcttt
     3781 agtatttatt gctctcaatc atttataacc accgaaaagt atcttacttc tgttttagat
     3841 atgagacaat actacatatc aggcaccatc tacaggggct tctcgaggat tccggcgtcg
     3901 gttgacattt ataagacatc actgatacaa aagagatgga tctatcgatc tgaagcgagg
     3961 gtaggagcgt gggtcaacgc ggggaagtgt tttcaacaag gctcgtcaag aatgagcgga
     4021 catatttttt taatgtgaaa gagaatattc gaggggatgt gtttttgaac atcgtcgaat
     4081 cgaaaggcct gccggacagt gaccgattta ttcgccaatc gatcatcatc taccaggagg
     4141 acttgggcga gtttttgaaa gagcttcaga agtccctcga tttcatcaag cagaataccc
     4201 gttaatatct gaaactcaac cgatggatcg gagaaggccc gtattgtttc aaggcctctt
     4261 tatgtgcttt ggtcgggtat cctttgtgct cggcgaagcg atacatcggc cactgttcat
     4321 cgtaggcgac catgatccgg tcccgttcca ccttggcaag aattgaagcc gccatgatct
     4381 cggggatcga gtcatccccc tttacgattg tctccgtttc acaggagagg atcggcgagc
     4441 gattgccgtc aatcagggct ttggtgatcg ggtggttttt ctgtaattga gatactgcgt
     4501 tgtgcatcgc ttccagcgtc gcctgaagaa tattgatctc atcgatccgc cgatgcgaga
     4561 cgattgccac cgtccaacct gcagcctgtt ccttgatgag cgtttcaagc atcgtccgtt
     4621 gcttaggggc cagcttcttt gaatctttca ggaggtgggc agggaagtgc tttcccagga
     4681 ttaccgctgc ggcgaccacc ggaccggcca gagggcctcg accagcttca tcaaccccac
     4741 agatcaacgt ca
//
LOCUS       DAOPUZ010000025         4723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_18216_length_4723_cov_3.281063, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000025 DAOPUZ010000000
VERSION     DAOPUZ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18216_length_4723_cov_3.281063"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="PKN79_00925"
     CDS             complement(<1..1018)
                     /locus_tag="PKN79_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ94361.1"
                     /translation="MKRLLVTQGLLVDTEWTRHADILIEDGIIVRIAPKIEVGEIDGP
                     VEIVRADGLAILPGIIDAHTHYHLVSRNTVTADSFAEGSRAAAYGGVTTVVDFADDDK
                     KRSLLVNAKERIAAMGKEMAIDFSLHQGIYSYRPSLKEELLELKEAGVKVIKLFTTYK
                     NVGYMIEKEEELKKIFAICKELDLLVTIHAEDDEVIQRAEKAYKGDYAPASHPILRPS
                     EAEAVGIRTMGKIALEVGIPIYIVHLSSKAGLEAVRDLRRQGLRVIVETTPHYLFLTG
                     EKLVGADGALWVMTPPLRDEEDRLALQEALLNGEIQVVATDHCAFTKEQKLSSKDSRT
                     ILPGI"
     gene            complement(1116..1391)
                     /locus_tag="PKN79_00930"
     CDS             complement(1116..1391)
                     /locus_tag="PKN79_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNZ94362.1"
                     /translation="MSKAVFVHDARVMSKGQVTIPKDVRTVLGVESGDRVTFIVEGNN
                     VRVVNSAVYALIRFQEQMGGEAEKAGFLTEEAVAEWITRSRRAEHVE"
     gene            complement(1487..2938)
                     /gene="gcvPB"
                     /locus_tag="PKN79_00935"
     CDS             complement(1487..2938)
                     /gene="gcvPB"
                     /locus_tag="PKN79_00935"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNZ94363.1"
                     /translation="MIEPLLIDKSKKGRLAYCYPSLDLPRSFDQEFAYLPSHLQREGS
                     ARLPEVSELDVVRHFTRLSRMVHGVDNGMYPLGSCTMKYNPKLGEEIARMAQFTEIHP
                     LQDISTVQGCLSVMYNLLEDLSSITGMSWGSLQPCAGSHGEYTGLKMIRSYFLKKGDV
                     GRTKVLIPVSAHGTNPASAAVNGFDVVEIASNRKGLVDLADLKSKLSDEVAALMLTNP
                     NTLGLFEEDILEISEAVHEAGALLYYDGANLNAIMGKVRPGDMGFDVLHLNLHKTFST
                     PHGGGGPGSGPVMVNDLLRPFLPKPDIDLREGGYVFDWDRPDSIGKVSMFWGNFLVFL
                     RAYVYILRMGSEGLRAAANHAVLNANYLAKKLAPYYEIPHTGGIMHEFVISVKNYKEQ
                     YGVTAEDFAKALIDEGYHPPTVYFPLIVDEALMIEPTESESLESLDAFIAALIRIAEK
                     AKSDPASLKKAPVSTPVGRLDQVKAVKDAKVRY"
     gene            complement(2935..4284)
                     /gene="gcvPA"
                     /locus_tag="PKN79_00940"
     CDS             complement(2935..4284)
                     /gene="gcvPA"
                     /locus_tag="PKN79_00940"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNZ94364.1"
                     /translation="MIYPYIPHTDEDRAQMLEALGLSSVDRLFEVVGEDLLLEDSVPI
                     AHRRTEDEVQRMIDSIAQRNIRGISFLGGGCYDHIIPSTVKALSSLPSFVTAYTPYQA
                     EMSQGLLQAIFEFQSMICEITSMDIANASLYDGSHAIVEAALLMVNAKRGAKKVLVSE
                     TIHPFALKALGTWVLGTEYEVVPIREKDGVVDLSGLVIGDDVGGLIVQSPNRYGFVED
                     YSGIADLLHEHKALFAISSDPLSLAIQKTPGQWGADIAVGDTQSLGLGLSFGGPSCGY
                     MAVKQPLLRRIPGRLVGITTDAEGRRGFTLTLQAREQHIKRERATSNICSNQALAALM
                     TTIHLSSLGWHGMVEAARQSYAKAHYLAHHLGQLPGVEVMNAHPFWCEFPVVFHDAKR
                     MRKFLQELRNEGIFAGVRLSALTRKDTDELVLLVAVTEKRSREELEMYIAAAQRVMK"
     gene            complement(4304..4687)
                     /gene="gcvH"
                     /locus_tag="PKN79_00945"
     CDS             complement(4304..4687)
                     /gene="gcvH"
                     /locus_tag="PKN79_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNZ94365.1"
                     /translation="MSRILDTLRYSKDHEWVRIEGEKAYVGITDHAQDELGEIVFVEL
                     PPIGERYKKDEEISSIESVKAASPIINPIEGVVLEANEDLDGSPELINEDCYANHLYV
                     LGSFSQEDYDSMMDSTEYASYLASL"
BASE COUNT         1047 a         1357 c         1238 g         1081 t
ORIGIN      
        1 ggataccggg aaggatcgtc cggctgtcct tggaggaaag cttctgttct ttcgtgaagg
       61 cacagtggtc ggtcgcgacg acttggatct ctccgttcag gagcgcttcc tgcaacgcca
      121 accgatcttc ctcatcccga agcggagggg tcatcaccca cagcgccccg tcggcgccca
      181 caagcttctc gccggtcagg aacaggtagt gcggcgtcgt ttcaacgatc acccgcagcc
      241 cttgccttcg aaggtcgcga accgcctcca gccctgcttt actggagagg tggacgatgt
      301 agatcgggat gccgacctca agggcgatct tccccatcgt ccgaattccg accgcttccg
      361 cttccgaagg acggagaatc ggatgggaag cgggagcata atcacctttg tatgcctttt
      421 cagcgcgttg gatgacctcg tcatcctccg cgtggatcgt aacaagaaga tcaagctctt
      481 tacagatcgc aaagatcttt ttcaattcct cttctttttc gatcatgtac cccacgttct
      541 tgtaggtagt gaagagcttg atgaccttga ctccggcttc tttgagctca agcagctcct
      601 ctttcaggct tgggcggtag gaataaatcc cttgatggag ggaaaaatcg atcgccatct
      661 cttttcccat cgccgcaatc cgttctttgg cattgaccaa gagcgatcgc ttcttgtcat
      721 cgtcggcaaa gtccaccacg gtggtaaccc cgccataggc ggcggcccgc gagccctcgg
      781 cgaaggagtc ggcggtaacc gtgttgcgac ttaccaaatg atagtgggta tgcgcatcga
      841 tgataccggg aagaatcgca agcccgtcgg cccggacgat ctccaccgga ccgtcaatct
      901 caccgacctc gatcttcgga gcgatccgaa cgatgattcc atcctcgatc aggatgtccg
      961 catgccgggt ccactcggta tccaccagca aaccttgggt aaccagaagt cttttcatac
     1021 acctaggata ctccctggag gagcaattgg caacatagga gatatcaagc aaaaggaacc
     1081 ggatgacccg aatatgtcgg tgtcaatcat gatcttcatt cgacatgttc cgcacgacga
     1141 gaccgggtaa tccattccgc cactgcttcc tcagtgagaa aaccggcctt ctccgcctcg
     1201 ccgcccatct gctcttgaaa gcgtatcaaa gcgtaaacgg ctgaattaac aactcgcaca
     1261 ttgttccctt caacgataaa tgtaacgcga tcgccactct ccacaccaag cactgtacgt
     1321 acgtccttcg ggatggtaac ctgacctttt gacatgaccc gtgcgtcatg tacgaatact
     1381 gcctttgaca tataaatatc tcctaaagaa gtatggaaat agggatttcc tactttttag
     1441 tatacgtatt ctgagaaaaa atcaattagg caactgcatt cagtactcaa taccggacct
     1501 tcgcgtcctt gacggctttt acctggtcga gccggcctac cggggtcgaa accggggcct
     1561 tcttcagcga ggcgggatcg cttttcgctt tttccgcgat gcggatcagc gcagcgatga
     1621 acgcgtccag actctcaagg ctttcgcttt cggtgggttc gatcatcagc gcctcgtcga
     1681 cgatcagggg gaagtacacc gtcggggggt ggtacccttc atcaatcagc gctttggcga
     1741 aatcctcggc ggtgactcca tactgctctt tgtagttttt gacgctgatc acaaactcgt
     1801 gcatgattcc accggtatgg ggaatctcat agtacggcgc caattttttt gccagatagt
     1861 tggcgttcag caccgcatga ttggcggcgg ctcgaaggcc ttcgctcccc atgcggagaa
     1921 tgtagacata cgcccgcaga aaaacgagga agttccccca gaacatgctc accttcccga
     1981 tcgaatccgg cctgtcccaa tcgaacacat accccccttc ccgcagatcg atatccggct
     2041 tcggaaggaa cgggcggaga agatcgttga ccattaccgg tccgctgccc gggccgccgc
     2101 cgccatgggg ggtggagaac gttttatgaa ggttgaggtg gagcacgtcg aagcccatgt
     2161 cgccgggacg gaccttgccc atgatcgcat tgaggttcgc cccgtcatag tacagcaggg
     2221 ctcccgcttc atggaccgct tcgctgattt caaggatatc ctcttcgaaa agtccgaggg
     2281 tgttggggtt ggtcaacatg agggcggcga cctcatcgga gagcttgctc ttcaaatcag
     2341 ccagatccac caatcctttc ctgttcgaag cgatttcaac cacatcgaag ccgttgaccg
     2401 ccgcgctggc ggggttggtc ccgtgggcgc tgacgggaat cagaaccttg gttcgcccaa
     2461 cgtcgccctt tttcaaaaag taggagcgaa tcattttaag accggtgtat tcgccgtggc
     2521 tgccggcaca cggctgaagc gatccccagg acattccggt aatggaggaa aggtcttcca
     2581 ggaggttgta catcaccgat agacaccctt gaaccgtcga gatgtcctga agtggatgga
     2641 tctcggtgaa ttgagccatc cgggcgatct cttccccgag cttcggattg tatttcatcg
     2701 tacaggagcc caaggggtac atcccgttgt cgaccccgtg aaccatcctc gaaagccggg
     2761 tgaagtgcct gaccacatcg agctcggaga cttccggcag gcgggcgctc ccctcccgtt
     2821 gaagatggga cggaagatac gcaaactcct ggtcgaagga ccggggaaga tccagggaag
     2881 gataacaata cgccaagcgc ccttttttgc ttttatcgat caataacggt tcaatcattt
     2941 catcaccctc tgagccgccg caatatacat ctccagctct tcccggcttc tcttttccgt
     3001 taccgctaca agaagaacga gttcatcggt atctttgcgg gtcaacgcgg aaagccggac
     3061 accggcaaag atcccttcgt tgcggagctc ctgaaggaac ttccgcatcc gcttcgcatc
     3121 gtgaaagacg accggaaact cgcaccagaa cggatgggca ttcattacct ccaccccggg
     3181 aagctgaccg agatgatgag ccaggtagtg cgctttggcg taggattgcc gggccgcttc
     3241 gaccatccca tgccacccga gcgatgaaag gtggatcgtc gtcatcaggg cggcaagcgc
     3301 ctggtttgaa cagatgttgc ttgtcgcccg ctcccgcttg atgtgctgtt cccgtgcttg
     3361 gagggtcaga gtgaagcctc ttcttccttc ggcgtcggta gtgattccga caaggcgacc
     3421 cggaattctt cgtaacagcg gttgtttcac cgccatgtag ccgcaggacg gaccgccaaa
     3481 agaaagaccg agacccagac tttgggtatc gccgacggcg atgtccgctc cccactggcc
     3541 gggagtcttt tgaatcgcca ggctcagcgg gtcgctgctg atcgcaaaga gcgctttgtg
     3601 ctcgtgaagc agatccgcta taccgctata gtcttcgaca aatccgtagc ggttgggcga
     3661 ttgcacgatg agcccgccga catcatcgcc gatgacgagg cctgaaaggt cgacaacgcc
     3721 atccttctcc cgaatcggta caacctcgta ctccgttcca aggacccacg ttccgagcgc
     3781 cttgagggcg aaggggtgga tcgtctcgct gaccagcacc ttcttggcgc cccgcttggc
     3841 gttcaccatc aaaagcgccg cttcgacgat cgcatgtgaa ccgtcgtaca gcgaagcgtt
     3901 cgcgatatcc atcgaggtta tttcacagat catcgattgg aactcgaaga tcgcctgaag
     3961 caatccctga ctcatctccg cctggtacgg ggtgtaggcg gttacaaagg aaggaagtga
     4021 agaaagcgcc ttgacggtgg aaggaataat atggtcgtaa cacccgccgc cgagaaacga
     4081 gattcctcgg atgtttcgtt gagcgatcga atcaatcatc cgctgcactt catcctcggt
     4141 tcgcctatga gcgatcggaa ccgaatcttc gaggagcaga tcctcaccga ccacctcaaa
     4201 gagccggtcg accgatgaaa gaccgagcgc ctcaagcatc tgcgcccgat cctcatccgt
     4261 gtggggaatg taggggtaaa tcatgtaata ctccgcttct tgcctacagt gaggcaaggt
     4321 aggatgcgta ttcagtgctg tccatcatgg aatcgtaatc ttcttgagaa aaagacccga
     4381 gaacgtacag gtggtttgca taacagtcct cgttgatcaa ttccggcgag ccgtcaagat
     4441 cttcattcgc ctcaaggacg actccttcaa tcgggttgat gatcggactg gctgctttta
     4501 ccgactcgat cgaggagatc tcttcatctt ttttataccg ctcgccaatc gggggcagtt
     4561 cgacaaacac gatctccccg agctcatcct gggcgtggtc ggtgatgccg acataggctt
     4621 tttcaccttc aatgcgaacc cattcatgat ctttgctata gcgcaatgta tctaaaatcc
     4681 tgctcatctt gctcctccgc gtaacgtttc aatgctacca ttc
//
LOCUS       DAOPUZ010000026         4700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_18330_length_4700_cov_3.639397, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000026 DAOPUZ010000000
VERSION     DAOPUZ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18330_length_4700_cov_3.639397"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /gene="rhaS"
                     /locus_tag="PKN79_00950"
     CDS             <1..819
                     /gene="rhaS"
                     /locus_tag="PKN79_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ94366.1"
                     /translation="VYMGPSQPTAEGQIEIIEALIARRVDGIAISANDADALIPVAKK
                     AMAAGIKVISFDSGINVGGRIVDLLPSNAELIGRQQIQLAAELANSAGDVAVLSASAQ
                     ATNQNLWIDWMKEEIKDPKYSKMKLVEVVYGDDLPDKSYREAVALMKKYPNLKAIICP
                     TTVGLLATAQAVKDEGKAGIVEVTGLGLPSEMKGYILDGTCRQMALWNPIDLGYTSTY
                     LLNALIDGTKKGSVGEEIPAGRMGNMKVLEDGIIFMSTPYVFNKDNVEMFAAYF"
     gene            896..2407
                     /locus_tag="PKN79_00955"
     CDS             896..2407
                     /locus_tag="PKN79_00955"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNZ94367.1"
                     /translation="MNQPLVKVQNLTKVFPGIRALDDVRIEIRTGEVHALIGENGAGK
                     STLVKILTGVYQPTGGSVFLEGEEITFRNAIDAQEAGIVAIHQEASMFPELSVTENIY
                     MGHHVRHPKTRKLDWKQMRKETQALLARMQLSINPDTLVKNLSVAQRHMVEIAKALSL
                     DAKLVIMDEPTSALTDREVEDLYRIVRQLKAEGKAILFISHKFSEIFAICDYYTVLRD
                     GQYIGEGRIDRSSEDEIIRMMIGRSLDQLYPDHVPAWGKEILRVDSLCQLGAFRNVSF
                     TLREGEILGLFGLIGAGRSEVVRTIFGVDKASSGTITFEGKPFMPKSPYDAMRRGIAL
                     VPEDRQKQGLILKMSLTHNISLPVLDALSWKRSITRKKEEATYVLDHGQRMEIRAAGY
                     HVDAETLSGGNQQKVVLAKWIGTEPKVLILDEPTKGIDVATKAAVHEFICEMAKRGVA
                     IILISSELPEVIGISDRILVMHEGRQTAILDKSEANAETVMRWAISTEKRANA"
     gene            2400..3392
                     /locus_tag="PKN79_00960"
     CDS             2400..3392
                     /locus_tag="PKN79_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94368.1"
                     /translation="MPSNTIKKILARREAALVILLVVLLIPITIRSPQFISARNISTI
                     LNDMAILAIVAIGEFFVILSNGIDLSVGSVVAFTGMACGLINEANQAFGAVPLLFVGM
                     GIGLVMGLFNGVLVAYGKIPPIITTLGTVSIYRGLTFLLSGNQWVTAHELAPSYIAFP
                     RVTFLGISLLLWIAFIVIAIFYYFSRFTRTGREIYAIGGNPTAAKFVGVNESRIRVVV
                     FVISGTLSGLAGALWTARYASAVNEMATGFEMQAVAACVLGGVNFAGGAGAITGVVLG
                     TLFLGVVTNALPVVYLSVFWQTFVQGLIILVALTLNTLSDRRKHTKLLAQRRAS"
     gene            3392..4432
                     /locus_tag="PKN79_00965"
     CDS             3392..4432
                     /locus_tag="PKN79_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94369.1"
                     /translation="MREKRELMAKTRLIDESSLKERLITIFTMWEVLLSIIFVFIIIF
                     FSTRTRYFLNWFNLMNATFQFSEKAIMALPMIFIIMCGDIDISIASIIALCAYAVGNA
                     AAGGASIPTLILIALAVGTLAGFFNGFMVTFLEMPAIAVTLATQSIYRGIAIGMLGDQ
                     ARTTFPKTYGFFGQEFIKGTTIPFEFVLYLVLMVIFGFILHKTTYGRRLYAIGNNAEV
                     ARFSGINVKRIRIINFTITGFISGLTAILLTSRVLSVRSNIATGWDMEIITLVVLGGV
                     SITGGKGTVWGVFLGSLLVGYLKFGMGLMKISGTLMTIVIGSLLIIAVLLPRLLDLYK
                     ANRKLRKQHEMR"
     gene            4442..>4700
                     /locus_tag="PKN79_00970"
     CDS             4442..>4700
                     /locus_tag="PKN79_00970"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HNZ94370.1"
                     /translation="MREAFVMYLKKGHYFLKEKRAYEIWPELAEALSAAGISDYSIFL
                     DEESLTLFAVQKLSETNTAAQLPQTAIMQKWWAYMAPLMEVN"
BASE COUNT         1067 a         1275 c         1317 g         1041 t
ORIGIN      
        1 gtatatatgg gacccagcca gccgaccgct gaaggacaga tcgagatcat cgaagcattg
       61 atcgcccgac gcgtcgatgg aatcgcgatc agcgcgaacg acgctgacgc cctgattccc
      121 gtcgcgaaga aagcgatggc cgcaggaatc aaagtgatca gctttgacag cggaatcaac
      181 gtcggtggac gaatcgtcga cctgctcccc tccaatgcgg agttgatcgg ccgccagcag
      241 attcagcttg ccgctgagct tgccaacagc gccggagacg tcgctgtgct ttccgccagc
      301 gcccaggcga cgaaccagaa cctctggatc gactggatga aggaagagat caaggatccc
      361 aagtattcga agatgaagct tgttgaagtc gtctatggcg atgaccttcc cgacaagagc
      421 taccgcgaag ccgtcgctct gatgaagaag tacccgaacc tcaaagcgat catctgtccg
      481 accaccgttg gacttttggc aaccgcccaa gcggtgaagg atgaaggaaa agccggcatc
      541 gtcgaagtca ccggtcttgg actcccctcc gaaatgaagg gatacatcct cgatggcacc
      601 tgccgtcaaa tggccttgtg gaacccgatc gatctcggat acacctccac ctatctgctc
      661 aatgccctca tcgacggcac gaagaaaggc agtgtcggtg aagagattcc cgccggtcgc
      721 atgggaaaca tgaaagttct cgaggacggt atcattttca tgagcacccc gtacgtcttc
      781 aataaagaca acgtcgagat gttcgctgcg tacttctaag aggtactccg atcgggttct
      841 tcacgaggtg gggagcccga tcgatgtttg ttgttcttga tgtgaggagg aacgagtgaa
      901 ccagccattg gttaaggtcc agaacctgac gaaagtattt cccggaatcc gagcgttgga
      961 tgatgtccgc atcgagattc gcaccgggga agtccacgcg ttgatcggag aaaacggagc
     1021 gggaaaatcc acccttgtaa aaattttgac cggagtgtat cagcctaccg gcgggagcgt
     1081 gttccttgag ggagaggaaa tcacctttcg aaacgccatc gacgcccaag aagccggaat
     1141 cgtcgccatt caccaggagg cttcgatgtt tcccgagctc agcgttacgg aaaacatcta
     1201 tatggggcac cacgtgcgcc atcctaaaac tcgaaagctt gattggaagc agatgcgcaa
     1261 ggagacgcag gcacttctgg cccgaatgca gctttcaatc aatcccgata cattggtaaa
     1321 gaatctcagc gtcgcccaac ggcacatggt tgagatcgcc aaagccctca gccttgacgc
     1381 caagctcgtc atcatggatg agcctacctc cgctcttacc gatcgcgagg ttgaggatct
     1441 ctatcgaatc gtccgtcagc ttaaagcgga gggaaaggcg attcttttca tctcccataa
     1501 gttcagcgag atttttgcga tttgtgacta ctacaccgtc cttcgggatg gtcaatatat
     1561 cggcgaaggc cgaatcgacc gctcgagcga ggatgagatc attcgaatga tgatcggccg
     1621 ctcgcttgat cagctttatc ccgaccatgt ccctgcttgg ggaaaggaga ttctccgggt
     1681 cgattcgctt tgccaattgg gagcgttccg gaacgtttcc ttcaccctcc gcgagggtga
     1741 gattttgggt ctgttcggcc tgatcggagc gggccgcagc gaagtggttc gaacgatctt
     1801 cggagtcgac aaggcctcga gcggaacgat cacctttgaa ggaaagccct tcatgcccaa
     1861 gagcccgtat gatgcgatga gacgggggat cgcgttggtc cccgaggacc gccagaagca
     1921 aggcctcatc ctgaagatga gcctgacgca caatatctcc ttgccggtcc ttgacgccct
     1981 ttcgtggaag cgttcgatca cccggaagaa agaagaggcg acctatgtgc ttgaccacgg
     2041 ccagcggatg gagatccgag ccgccggcta tcacgtcgat gccgagaccc tcagtggggg
     2101 aaaccagcag aaagtcgttc ttgccaaatg gattggcacg gagccgaaag tcctgatcct
     2161 tgacgagccg accaagggga tcgatgtcgc caccaaagct gccgttcatg agttcatctg
     2221 cgagatggcg aagaggggag ttgcgatcat cctcatttca agcgagctgc ccgaggtgat
     2281 cggaatcagc gaccggattc tggtgatgca tgaaggcaga cagacagcga ttctcgacaa
     2341 aagcgaagcg aacgccgaaa cggtaatgcg ctgggcgatt tcaacagaga agagagccaa
     2401 tgcctagtaa tacgataaag aagatccttg cccgccgcga agctgcattg gtgattttgt
     2461 tggtggtatt gttgattccg attacgatcc gctcgccgca gtttatttca gcacggaaca
     2521 tctcgacaat tctcaatgac atggcgattt tggcgatcgt cgcgatcggc gaattcttcg
     2581 tcatcctttc caatggaatc gacctgtcgg tcggttcggt cgtcgccttc accggaatgg
     2641 cgtgcggact gatcaacgaa gcgaatcagg ccttcggtgc ggttccgctt ctgtttgtcg
     2701 gaatgggaat cggtcttgtg atggggctct tcaatggagt ccttgtcgcc tacgggaaaa
     2761 tccctccaat catcacgacg ctcggaacgg tgagcatcta ccggggcctc acgtttttgt
     2821 tgagtggaaa ccagtgggtc accgcccacg aactcgcccc ttcctatatc gcatttcccc
     2881 gggttacctt cctagggata tcgctgctcc tttggatagc gttcatcgtc atcgcgatct
     2941 tctactactt cagccgcttc acccgaaccg gccgggagat ctacgcgatc ggaggaaatc
     3001 ccactgcggc gaagttcgtc ggagtgaatg agagcaggat ccgggtggtc gtctttgtaa
     3061 tcagcggaac gctcagcggg ctggcgggag ccctttggac agcccgctat gcatcggcgg
     3121 tgaacgagat ggctaccggc ttcgagatgc aagcggtcgc cgcctgcgtg ctcggaggag
     3181 tcaacttcgc cggcggggcc ggagcgatca ccggggtggt tctgggaacg cttttcttag
     3241 gggttgtgac caacgccctt ccggtcgtct atctttccgt attctggcaa accttcgtcc
     3301 aaggtctcat cattctcgtc gcgctgactc tcaacactct tagcgatcgt agaaagcata
     3361 ccaagctgtt ggcacagagg agggcatcgt aatgagagaa aaacgcgaac tgatggcaaa
     3421 aacccgactc atcgatgaaa gttccctgaa agaacgattg atcacgatct tcaccatgtg
     3481 ggaagtcctt ctgtcgatca tcttcgtttt catcatcatc ttcttctcga cccggacccg
     3541 gtactttctt aactggttca acctgatgaa cgctaccttc caattcagtg agaaggcgat
     3601 catggccctt ccgatgatct tcatcatcat gtgcggcgac atcgatattt caatcgcctc
     3661 gatcatcgct ctctgcgcgt acgcggtggg caacgcagcg gccggtgggg cttcaattcc
     3721 gacgctgatc ctcatcgctc tcgctgtggg tacgctggcg gggtttttca acggattcat
     3781 ggtcacgttc ctcgagatgc cggcgatcgc cgtgacgctg gcgacgcaat cgatctaccg
     3841 gggaatcgcg attggaatgc tcggcgacca ggctcgaacg acgtttccga aaacctacgg
     3901 atttttcggt caggaattca tcaaaggcac cacgatcccc tttgagttcg tgctctacct
     3961 cgtgctcatg gtgatcttcg gcttcatcct ccacaagacg acctatggaa ggcggctcta
     4021 tgcgatcgga aacaacgccg aagtcgctcg gttcagcgga atcaacgtca agcggattag
     4081 gatcatcaac tttacgatca ccggcttcat aagcggcctt accgccattc tgttgacatc
     4141 ccgagtcctc tcggtccgct ccaacatcgc caccgggtgg gatatggaaa tcatcactct
     4201 ggtcgtgctc ggcggcgttt cgatcaccgg tggaaaggga acggtgtggg gcgtgttcct
     4261 cggatcgctc ctcgtcgggt atctgaagtt cgggatgggg ttgatgaaga tctcgggaac
     4321 gttgatgacg atcgtcatcg gatcgcttct gatcatcgcg gttctccttc cccgcctgct
     4381 cgacctgtac aaagccaacc gcaagctgcg caagcagcat gagatgcgat aggaggaagc
     4441 catgcgtgaa gcgtttgtga tgtacctgaa aaagggacat tattttttaa aggagaagag
     4501 ggcatacgag atctggcctg aactggccga ggcactgagc gcggcaggta tctccgacta
     4561 ctcgatcttt ctcgatgagg agagcctgac gctctttgcc gtgcagaagc tcagcgagac
     4621 aaacaccgcc gctcaattgc cgcagaccgc gatcatgcag aagtggtggg cctacatggc
     4681 gccgttgatg gaggtcaatc
//
LOCUS       DAOPUZ010000027         4670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_18477_length_4670_cov_2.963380, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000027 DAOPUZ010000000
VERSION     DAOPUZ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18477_length_4670_cov_2.963380"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /gene="hflX"
                     /locus_tag="PKN79_00975"
     CDS             <1..942
                     /gene="hflX"
                     /locus_tag="PKN79_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNZ94371.1"
                     /translation="VILQIFADRAQTKEAMLQVELARLQYSLPRLVRKWTNLSQQRGG
                     VRGSRGEGETRLELDRRRIQDRIALLKKQLLNVVQQREVQRSSRLKSPNPIGAIVGYT
                     NSGKSTLLNTLTEAGVLAEDKLFATLDPTTRLFKLPGGEEVLLSDTVGFISDLPHHLV
                     DAFKSTLEEARYADFLIIVCDASHPDMIATYTTTVQVLEDLGVTDKPAIVVANKIDKV
                     EDFFALSRLKSLYQPVLETSLKSGQGLETLIDAITETIHTLNPTLSYKFPNDRHDLVA
                     YLHRSGQVANISYTEEGIIVKARLAERFASSLSSYRV"
     gene            999..2579
                     /locus_tag="PKN79_00980"
     CDS             999..2579
                     /locus_tag="PKN79_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNZ94372.1"
                     /translation="MERHLTARAIIIGILGLLVITASSMYVALRMGALPWPTIFVTVL
                     SMAALKKARNSSLQEMNVTHTIMSSGAMVAGGLAFTIPGLWMLNPDAVFPPMSLITLT
                     VVGAILGTLFSALLRKRLIVEEELPYPMGIAAFNTLKASEEGGSGAKTLFTSMGGSVI
                     FTLLRDAWAKIPALLTPFKGSALFPSFSFWVSPMAAGIGAIIGPVFALFWFGGAIFGY
                     YLLTPIGIKAGLFGDISAADVFRQNLGIGLMIGTGLGVFIHALFSKILRPKKLEEKPK
                     KSQRTLFLISLVLLLTVVLLSLGTELGFVEALVLIVGIYLTTYLSGMLTGQTGINPME
                     IFGILVLLVIQLISKPSLLASFSIAAVVAVACGLTGDVMNDLKSGHLLGTNPREQILG
                     EGIGGVIGAVLSVIVLLAMKSAFGGFGTALLPAPQAAAVAKMVGGIEHLPAFLIGLFV
                     GLALFLMKIPSATLGLGVYLPIYISSIMGLGALASLIFTRKKDKEKSQKKIGLIASGL
                     LGGEGITGVLIAILSMLK"
     gene            2593..3633
                     /gene="rlmN"
                     /locus_tag="PKN79_00985"
     CDS             2593..3633
                     /gene="rlmN"
                     /locus_tag="PKN79_00985"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNZ94373.1"
                     /translation="MNLPLSLYGLTSERLVESLSLPEKRYGGQILGWLGKGATTFDEM
                     TDLPLDERKRLSDLVGSPISSETTVREEDESGTIKLGITLHDGKMIEAVLLVDRKGRH
                     TACFSVQVGCAMGCTFCKTGTMGLIRNLASEEIIEQYVHLSKAASRPITHIVFMGMGE
                     PFHNFDPTIRAIHYLNRKETFNIGLRRMTISTCGVVPGINKLAELKLPIKLAVSLVSA
                     DDSLRTRLMPVNKRWPLAELKEALLAYQESVPKRLTLEYCMLGGVNTTGESARQLELF
                     TRGLDVIVNLIAWNPIDELEFSTPSEEEQKAFSALLNRYRVKHTLRISRGRTIGGACG
                     QLATSHNPETKR"
     gene            complement(3693..>4670)
                     /locus_tag="PKN79_00990"
     CDS             complement(3693..>4670)
                     /locus_tag="PKN79_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNZ94374.1"
                     /translation="EDDMSIRILSDHVRTGVFILGDQKGVSPSNVGQGYILRRLIRRA
                     VRHAHKLGIEGSFLSELAEVVIELYKKPYPELLENREFVMKELVAEEAKFSATLAKGE
                     REFEKMLPNLLKSPNRIISGRTAFKLYDTYGYPIELTRELAAENGFEVDEKGFHEAFA
                     KHQELSRAGGDQTFKGGLADHSERTTALHTATHLLHQALRTVLGDHVGQKGSNITVER
                     LRFDFTHPEPMTQEQIQKVEAIVNEVIRRDLPVSFTTMSLEEARQSGAIALFEGRYDE
                     QVKVYSVGDFSKEVCGGPHVERTGGMGTFKIQKEQSSSAGVRRIRATLE"
BASE COUNT          994 a         1408 c         1255 g         1013 t
ORIGIN      
        1 gtgattctgc agatcttcgc cgatcgcgcc cagacaaagg aggcgatgct gcaagtcgaa
       61 ctcgctcggc tccaatactc ccttccccgc ctggttcgca agtggacgaa cctttcacag
      121 caacgcggag gagttcgggg atcccgaggc gaaggtgaaa ccagactcga actggaccga
      181 cgccgaattc aggaccggat cgccctcctc aagaagcagc ttctcaacgt agtccaacag
      241 cgggaggtgc aacgctcctc ccgcttaaag agccccaatc cgatcggagc gatcgtgggc
      301 tatacgaaca gcggaaaatc aacgctgctc aacaccctca ctgaagcggg agttcttgct
      361 gaggacaagc tttttgcgac cttggatccc acgacccgcc tcttcaagct ccccggaggg
      421 gaagaagtgc tcttaagcga cacggtcggc tttatcagcg atctgcccca ccatctggtc
      481 gatgcgttca aatccacgtt ggaggaagcc cggtatgcgg attttctcat catcgtctgt
      541 gatgcaagtc atcccgacat gatcgccacc tacaccacca cggttcaggt tttggaagat
      601 ctcggcgtta ccgacaagcc tgcgattgtt gtggccaaca agatcgacaa ggtcgaggac
      661 ttcttcgcgc tttcccgcct caagagcctc taccaaccgg ttctggaaac atccctgaag
      721 agcggtcagg gattggagac gctgatcgat gcgatcaccg agacgatcca cactttgaat
      781 ccgacccttt cctacaagtt ccccaacgac cgtcatgacc tcgtggcata tctccatcga
      841 agcggacagg tagcaaacat ctcctacacg gaagaaggaa tcatcgtaaa agcccgcctc
      901 gccgaacgct tcgcttcctc gctgagttcc taccgagtct gactcttcca cccgctatcc
      961 tttctcgtta ctattacaca tatggaggaa gtgtatctat ggaacgacac ctcaccgcac
     1021 gtgcaatcat cattggaatc ttgggattgc tggtcattac cgcatcctcg atgtatgtcg
     1081 ccctcagaat gggcgccctt ccgtggccga cgatcttcgt caccgttctt tcgatggccg
     1141 cattgaaaaa agcacgcaac tcctccctgc aagagatgaa tgtcacccat acgatcatga
     1201 gcagcggggc gatggtcgcc ggtggactcg ccttcacgat tcccggcttg tggatgctca
     1261 atcctgatgc cgtgttcccg ccgatgagcc tcatcaccct caccgtggtc ggagcgatcc
     1321 tgggcactct cttctccgcc ctcctcagaa aacgcctgat tgttgaggag gagctgccct
     1381 atccgatggg catcgccgcg ttcaacaccc tcaaggcttc cgaagaagga ggtagcggtg
     1441 cgaagacgct ctttacgtca atgggaggat cggtcatctt taccctccta agggacgcgt
     1501 gggcaaagat accggcgctg ctcactccct tcaagggaag cgctcttttt ccttccttct
     1561 ccttctgggt ctcgccgatg gcggcgggaa tcggggcgat catcggcccg gtcttcgccc
     1621 ttttctggtt cggcggagcg atcttcggct actatctcct cactccgatc ggaatcaagg
     1681 ccggtctttt cggcgacata tccgctgcgg atgtcttcag gcaaaacctc gggatcggac
     1741 tgatgatcgg aacggggttg ggcgtcttta tccacgccct cttttcaaag attcttcgac
     1801 cgaaaaagtt ggaagagaag ccgaaaaaga gtcaacgaac gctctttctc atatcgctcg
     1861 tcctgctcct gacggtggtt cttctttcat tggggacgga gctgggcttt gtcgaggcgc
     1921 tggtgctcat cgttggcatc tatctgacga cctacctctc cgggatgctc accggacaga
     1981 cgggcatcaa cccgatggag atcttcggca tcctcgtgct gctggtgatc cagctgatct
     2041 ccaagccttc cctcctcgcc tccttctcga tcgcggcggt ggtcgccgtc gcctgcggct
     2101 tgaccgggga cgtgatgaat gacttaaaaa gcggacacct cctcggaacc aatccccggg
     2161 agcagattct cggtgaagga atcggagggg tgatcggggc ggttctctcg gtgatcgtcc
     2221 tcttggcgat gaagagtgcg ttcggcggct tcggcaccgc cctgctgccc gcccctcagg
     2281 cggcggcggt ggccaagatg gtcggcggaa tcgaacacct gccggccttc ctgatcggcc
     2341 tcttcgtcgg gttggcgctc tttctgatga aaataccctc cgccaccctg ggcctcggcg
     2401 tgtatctgcc gatctacatc tcttcgatca tgggccttgg cgcactcgcc tctttgatct
     2461 tcacccgaaa gaaagacaag gagaaatccc agaaaaagat cggattgatc gccagcggac
     2521 tgcttggcgg agaaggaatc accggcgtgc tgatcgctat tctttcaatg ctcaaatagg
     2581 tatactgaat ccatgaatct tcccctttcc ctctacggtc tcacttccga acgcctcgtc
     2641 gaatcactgt ccctccccga aaaacgctat ggcgggcaga tccttggctg gctcggtaag
     2701 ggagccacta cgtttgacga gatgaccgac cttcccctcg atgagcgaaa gcgcctaagc
     2761 gacctcgtcg ggagtccgat ctcatcggag acaacggtcc gagaggagga tgaaagcgga
     2821 acgatcaagc tcggaatcac gcttcacgac ggaaaaatga tcgaagcggt cctgctcgtc
     2881 gaccggaagg gacggcacac cgcgtgtttc tccgtccagg tgggatgtgc gatggggtgc
     2941 accttctgca agaccggaac gatgggcctg atacgcaacc tcgcctccga agagatcatc
     3001 gaacagtacg tccacctttc caaggcggca agccggccga tcacccacat cgtcttcatg
     3061 gggatgggcg aaccgtttca caacttcgat ccgacgatca gggcgatcca ctacctcaac
     3121 cggaaggaaa cgttcaacat cggcctcaga agaatgacga tcagcacatg cggagtggtt
     3181 cccggaatca acaagctcgc cgaattgaag ctcccgatca agctcgccgt ttcactggtc
     3241 agcgcggatg actcgctcag aacccgcctg atgccggtca acaagcgatg gccgctcgcc
     3301 gagctcaagg aagcgcttct tgcctatcag gaatcggtac ctaaacgact caccctcgag
     3361 tactgcatgc tcggaggcgt gaacaccaca ggtgaaagcg ccagacagct cgagctcttt
     3421 acccggggtc tggacgtgat cgtgaatctc atcgcctgga acccgatcga cgagcttgag
     3481 ttctccactc cgagcgaaga ggagcagaaa gccttctccg ccctcctcaa ccgctatcgg
     3541 gttaaacaca cccttcgaat ctcccgcggc cggacgatcg gcggagcgtg cgggcaactg
     3601 gccacttccc acaaccctga aaccaaaaga taagaatgcg gatgaagaaa aagaattggc
     3661 tgccggacac tgcctaacag ccaataaaac ggttattcga gggttgcccg aatccggcgg
     3721 actcccgccg aggatgattg ttctttctgg attttgaagg ttcccatccc gccagtccgc
     3781 tcaacgtggg gtcctccgca gacttccttg gagaagtctc cgacggagta gacttttacc
     3841 tgctcgtcgt agcggccttc aaagagggcg atcgcgcccg actgccgggc ttcctccaga
     3901 ctcatcgtgg taaagctcac cggaagatcc cgcctgatga cctcgttgac gatagcttcg
     3961 accttttgga tctgctcctg agtcatcggc tcagggtggg taaaatcgaa ccggagccgc
     4021 tcgaccgtaa tattcgatcc cttctgaccg acatgatcgc ccaagaccgt tctcagcgcc
     4081 tgatgaagga ggtgggttgc agtatgcagg gcggtggtcc gctcgctgtg atccgccaat
     4141 ccgcctttga acgtctggtc tcctccggcg cgactgagtt cctgatgctt ggcgaacgcc
     4201 tcatgaaacc ccttttcgtc gacttcaaag ccattttcgg cggcaagctc cctggtcagc
     4261 tcgatcggat agccgtaggt atcgtagagc ttgaacgcag ttcgaccgct gatgatccgg
     4321 ttcggactct taagcaggtt cggaagcatc ttttcgaact cccgctctcc tttcgccaag
     4381 gtcgcactga actttgcttc ttcggcgaca agctccttca tcacgaactc ccggttttca
     4441 agaagctcag gatagggttt tttgtagagc tcgataacga cttcggccaa ctcggaaagg
     4501 aaggaacctt cgatgccgag cttgtgggcg tgccggaccg cccggcgaat caggcggcga
     4561 aggatgtatc cctgaccgac gttggagggg gaaacacctt tctggtcgcc gaggatgaag
     4621 acgccggttc ggacatggtc ggagagaatc cggatcgaca tgtcgtcctc
//
LOCUS       DAOPUZ010000028         4619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_18755_length_4619_cov_2.948072, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000028 DAOPUZ010000000
VERSION     DAOPUZ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18755_length_4619_cov_2.948072"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="PKN79_00995"
     CDS             <1..1825
                     /locus_tag="PKN79_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94375.1"
                     /translation="AVENLRRELLQSIDSQANRTYVDQQITSLKNELTTSLGRLEEKQ
                     VDRRAIIDSITADDLRPAFSVFRDELLNQVRSEQPRTTVVMTGEVDEELLFKNLAWEI
                     ENNLDFYASYARDAILSTIDDDHMIALYESHRPMLLADLAASLPAPMTVIAAEPITGE
                     QVLSELTREIENNKEFYATYARDAILDTISEDDLFSYYNRYRPLLLGDLYQGIKDEAK
                     VEIADYADIIEQMEANREYYLAQDRKALPGVLTEADILPLYATYRDDLAEDLFTLLGE
                     EIESNKDAYAVEARDAILETISDDDLIAYYMQYRPALVADLMEELPAARAALDREQIL
                     DDLYAEIETNKPLYAAYVRDALLETMIGDPEFDQVYEENRVDIDAVLLALGEEIENNK
                     KFYATYVRNALLDTISGEELVNLYLNYRDALIEVGVELDANQIMGILTNEIESNREAY
                     ATWARKAIIDTMSDEELISYYLHYRPELLEDIYQGLKDRFAMASSAPVPSSPLPATQR
                     TVILQEAVEEEAAPPVVVPPSPSPAVQRTVILQEVVEEEAAPSVVVEEKQAPPAPRVP
                     VSPSVTTKPTTVPYEMTPAEYETLRQEKRNEALDEILKKIG"
     gene            complement(1822..2871)
                     /locus_tag="PKN79_01000"
     CDS             complement(1822..2871)
                     /locus_tag="PKN79_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNZ94376.1"
                     /translation="MNRDASITSLSALALKLTLSEEEKGFDESDGTLLPIKIPSSFFK
                     LINPDDPSDPIRRQVVPTVFEHTESRGETLDPLAEKRHAATARLIHRYPNRAAFLTTD
                     LCFIHCRHCFRRRFTGTFQGPANEEEIEQAARYVEKHPEITELLFTGGDLFTLNDVQL
                     ETMIGAFRSRRPDLIIRLCSRAPAARAERITPALIAMLKTFDTAPFFLMTQFNHVREL
                     TKEALRATALFIDNGIPAMNQTVLLKGVNDDADTLKELCNTILANRIKPYYLFQGDLV
                     SGTAHLRVSIEDGMKLEAELRKRLSGLAMPLYALDLPEGGGKVPLVNSYVEEKKDGAL
                     IFRTPYGGHRSYPAD"
     gene            2969..4273
                     /locus_tag="PKN79_01005"
     CDS             2969..4273
                     /locus_tag="PKN79_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ94377.1"
                     /translation="MTKQKKGLKGYYLTTFLIGLGFFTMGLMDPLYDTYVPIFLSRFV
                     ESKTLIGAIMTFDNILAIFLIPIFSALSDRVHTPIGRRMPFIVVCLPVSAVAFGLIPF
                     SALSSLWFLIILLFILNLFKQAVRGPVVALMPDMIPADLRSEANGVINTMGGIAAIVG
                     TVGLARLMDVPISLPGRGPTKDILAFPISGILVITATLLLFFFVKEKNAVAHDESERR
                     EPILSSLKAVFAEEDKSARAILFSLLFWFIGYQGILPFVGLYSTSILETSSGTASLAA
                     GMVGIAYAIFAIPSGIVAHRIGRKKTIRFSLGVLIVLLLLIFFHDPLTSAMGKAFRQY
                     TFWALLFFFGIFWVSIVTNSFPMLWQMSTYRTVGIYTGLYYFFSQLASIISPPLTGAC
                     IDIFGFRSIFVFGSAAMGIAFFIMRKVQGGEPEDQKSEEMSK"
     gene            complement(4253..4408)
                     /locus_tag="PKN79_01010"
     CDS             complement(4253..4408)
                     /locus_tag="PKN79_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HNZ94378.1"
                     /translation="MSMIGVITFTLCLLALYLGKTMGTRLSEHAERLGGVLLIILGCN
                     ILFTHLF"
     gene            4434..>4619
                     /locus_tag="PKN79_01015"
     CDS             4434..>4619
                     /locus_tag="PKN79_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94379.1"
                     /translation="MAKVIPTSESSWTEQAAYSGDVWTFVRDHIPYNRNAWENYRHPA
                     PIEGASGCIRVCMIHLEE"
BASE COUNT         1008 a         1235 c         1302 g         1074 t
ORIGIN      
        1 agccgttgaa aatcttcgcc gtgaattgct tcagtcgatt gacagtcagg cgaatcgaac
       61 ctatgtcgat cagcagatca cctccttgaa gaatgagctc accacctcgc ttggacgttt
      121 ggaggagaag caggtcgatc gtcgggcgat catcgattcg atcaccgccg atgatctgcg
      181 cccggcattc agcgtctttc gggatgaact cttgaaccag gttcgctctg agcaaccgcg
      241 aacaaccgtc gtgatgaccg gcgaggttga cgaagagctt ctgtttaaga atctcgcttg
      301 ggagatagaa aacaatcttg acttctatgc ttcatacgct cgcgatgcga ttctttccac
      361 gattgacgat gatcatatga tcgctctcta tgagagtcat cgccccatgc ttcttgcaga
      421 cttggcggcg agcttgccgg ctcccatgac cgtgattgcc gctgaaccga ttaccggcga
      481 gcaggtgctc agcgagctga cccgggagat tgagaacaac aaggaatttt atgcaaccta
      541 cgcccgtgac gcgatcctcg atacgatcag tgaggatgat ctcttctctt actataaccg
      601 ctaccgtccg cttctccttg gggatcttta ccagggaatc aaggatgaag cgaaagtcga
      661 aatcgccgat tatgccgata tcattgagca gatggaggcc aaccgcgagt actaccttgc
      721 tcaggaccgg aaggcgctgc ccggcgtcct aaccgaggcg gatatcctgc cgctgtatgc
      781 cacctaccgt gatgatcttg ccgaggatct ctttaccctt ctcggcgagg agattgaaag
      841 caataaggac gcctacgccg tcgaggctcg ggatgcgatt ctggagacga tcagcgatga
      901 tgacctcatc gcttactata tgcaatatcg gccggcattg gtggcggact tgatggaaga
      961 gctgcctgca gctcgagccg ctcttgaccg ggagcaaatc ttggatgatc tgtatgccga
     1021 gattgaaacc aataaaccgc tttatgctgc ctatgtccga gacgccttgc ttgaaacgat
     1081 gatcggtgat cccgagttcg atcaggtgta tgaagaaaac cgggttgata ttgatgccgt
     1141 cctcttggcc cttggcgaag agatcgagaa caacaagaag ttttatgcaa cgtatgtccg
     1201 aaatgccctc cttgatacga tcagcgggga agagcttgtc aacctctatc tgaactaccg
     1261 cgatgcattg atcgaagtgg gcgtcgagct ggatgcgaat cagatcatgg ggatccttac
     1321 caacgagatt gagagcaacc gggaagcata cgcaacatgg gctcgaaaag cgatcattga
     1381 tacgatgagt gacgaggagc tgatctccta ctaccttcac tatcgtcctg aactgcttga
     1441 ggatatctac caagggctga aagatcgctt tgccatggca tcttcggcgc ccgtcccgtc
     1501 aagcccgttg ccggcgaccc agcggacggt catcctccaa gaggcagtgg aagaagaggc
     1561 tgctcctccg gtggtcgtcc cgccaagtcc gtccccggcg gtccagcgga cggtcatcct
     1621 ccaagaggta gtggaagaag aggctgcacc ttcggtggtc gtcgaggaga agcaggcgcc
     1681 ccccgctccc cgggtgcccg tctcgccaag cgttacgacc aagccgacaa ccgttcccta
     1741 tgagatgacc cccgccgaat atgagacgct tcggcaggag aaacggaatg aagcgcttga
     1801 cgagatattg aaaaagatcg gctaatccgc cggataggaa cgatgtccgc catatggagt
     1861 cctgaaaatc agggctccat ctttcttttc ctcaacgtag gagtttacca gcggaacttt
     1921 tccaccgcct tcgggaagat cgagtgcgta gagcggcatc gccaagccgg aaaggcgttt
     1981 tcgaagctcc gcctccaact tcattccatc ttcgatcgaa accctcagat gagcggttcc
     2041 gctgaccaga tctccttgaa agaggtaata gggtttgatc cggttcgcta aaatggtatt
     2101 gcagagctcc ttgagggtat cggcatcatc attgacccct ttcagcaaca cggtttgatt
     2161 cattgccgga attccgttgt cgatgaaaag agcggtcgcc cggagcgcct ccttcgtcag
     2221 ttcccgaaca tggttgaact gggtcatcag aaagaacggg gcggtatcaa acgttttgag
     2281 catggcgatc aacgccggag tgatccgttc tgctctggcg gcaggagccc gagaacagag
     2341 gcgaataatc agatcgggtc gtcttgaccg aaacgctccg atcatcgttt ccaattggac
     2401 gtcattgagg gtgaagagat cccctccggt gaaaaggagt tcggtgattt caggatgctt
     2461 ttcaacatag cgagccgctt gttcaatctc ctcttcgttt gcaggacctt ggaatgttcc
     2521 ggtgaagcgc ctgcggaagc agtggcggca gtggataaaa caaaggtcgg tggtgaggaa
     2581 cgccgcccga ttgggatagc gatggatcag ccgggcggtg gctgcgtgcc gtttttccgc
     2641 caagggatca agggtttctc ctcgggattc ggtatgctcg aagacggtcg gaacgacctg
     2701 acggcgaatc ggatcggagg ggtcatcggg attgatcagt ttaaaaaagg aggaggggat
     2761 ttttatgggc aggagggtgc cgtcgctttc gtcaaagcct ttttcttctt cgcttaacgt
     2821 gagctttaaa gccaaggcgg acagggatgt gatggaagcg tctcgattca tgtctatggt
     2881 gtacttgaaa gaatcgcttt ggtctagggg tgaccggtcg cctacatttg ccgatccatg
     2941 atacaatggg cgcaatgagg agggtgctat gaccaaacaa aagaagggtt tgaagggata
     3001 ttacctcacg acgtttttga tcggactcgg gtttttcacg atggggttga tggatcccct
     3061 ctatgatacc tatgttccca tctttctttc ccgcttcgtg gaatccaaga cgctcatcgg
     3121 agcgatcatg acgttcgaca acattctggc gatctttctg attccgattt tcagtgctct
     3181 cagcgaccgg gtgcacaccc cgatcgggcg aaggatgccg ttcatcgtcg tctgtctgcc
     3241 ggtcagcgcg gtggcattcg gcctgattcc gttcagcgcc ctttccagcc tctggttcct
     3301 gatcatcctg ctcttcattc tcaacctctt caagcaggcg gttcggggac cggtcgtcgc
     3361 gctgatgccc gatatgatcc cggcggatct gagaagtgaa gcaaacggcg tgatcaatac
     3421 gatgggcggg atcgccgcga tcgtgggaac ggtcggtctt gcccgcctga tggatgtgcc
     3481 catcagcctc cccggacgcg ggccgacgaa ggatatcctc gcgttcccga tctccggaat
     3541 cttggtgatc accgcgaccc tcctcctctt cttcttcgtg aaggagaaga atgcggtcgc
     3601 ccacgatgag agcgagagac gggagccgat tctttcaagc ctgaaagcgg tcttcgccga
     3661 agaggacaag agcgcccggg ccattttgtt cagcctgctc ttctggttca tcgggtacca
     3721 gggaatcttg ccgtttgtcg gcttatactc cacctcgatc cttgagacct catcgggaac
     3781 ggcgtcgctc gcagcgggaa tggtcggcat cgcttatgcg atctttgcga tcccctcggg
     3841 aatcgtcgcc cacaggatcg gtcgaaagaa gacgatccgc ttctcccttg gggtgctgat
     3901 cgtcctgctg ctcctcatct tcttccatga tccgcttaca tccgccatgg ggaaagcgtt
     3961 ccgccaatat acgttctggg cgctgctctt cttctttgga atcttctggg tttcgatcgt
     4021 gacgaactcc ttcccgatgc tttggcagat gtccacctac cggacggtag gaatctacac
     4081 cgggctctac tatttcttca gccagctcgc ctcgatcatc agtccgccgt tgaccggcgc
     4141 ctgtatcgac atcttcgggt ttcgatcgat cttcgtcttc ggatccgctg cgatgggaat
     4201 cgcctttttc atcatgcgca aggttcaggg cggcgaaccg gaggatcaga agtcagaaga
     4261 gatgagtaaa taggatatta catcccaaga tgatcaggag cacgccgccg agccgctctg
     4321 catgctcgga aaggcgtgtt cccattgttt tccccagata gagcgcaagc agacacaggg
     4381 tgaaagtgat gactccgatc atcgacatgg cttgaacgag gttgatcttc aggatggcga
     4441 aggtgatccc gacgtcggaa tcttcgtgga ccgagcaggc cgcctactcg ggagacgtct
     4501 ggacgttcgt gcgcgaccac atcccgtaca acaggaacgc gtgggagaac taccgccatc
     4561 cggcgcccat cgagggggcg agcggctgca tccgggtctg catgatccat ctcgaggag
//
LOCUS       DAOPUZ010000029         4560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_19052_length_4560_cov_3.142952, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000029 DAOPUZ010000000
VERSION     DAOPUZ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19052_length_4560_cov_3.142952"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="PKN79_01020"
     CDS             complement(<1..1124)
                     /locus_tag="PKN79_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNZ94380.1"
                     /translation="MNESTSVGNRYIHIMLGIIVTLAVLAALKISKDVTIPLVLAFFF
                     FLMFSPLLRRLDKLHVPKLISTIFVMALMLLLLLAAGWFIVKTVDTLVRLIPFYTDKI
                     ISLDRLLTDMVNNFVEVPPSTSFLTLLPVNWSSLAISSLTQISNKFFSITKLALLVYI
                     FFLFLLLERQSVVPKLLAAVPQGKGMKVAVMFERITRQISKYLVIKIVISFFTGLFFY
                     LSALLTGLDLASLWGVLAFIFNFIPSIGSIVITSVVIFMSLVQFAPDWTNIILVAVLT
                     ISTQVILGNIIDPRLQGGQLNLSPFMILVALSLWGYIWGIVGMFIAVPLTSLMQILFA
                     NIKSLRPFAIMISSGKSYQRERARQKAIRSFQRRTQHRKS"
     gene            1179..1937
                     /locus_tag="PKN79_01025"
     CDS             1179..1937
                     /locus_tag="PKN79_01025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmJ/YjtD family RNA methyltransferase"
                     /protein_id="HNZ94381.1"
                     /translation="MTHPSRLDHIQIVLVDTQDGANIGSVCRAMKTMGITRLVIVGER
                     EYEENRIRTLALHAGDVWENRVQHPTLQEALKESSYTVAATRRRGKFRKHTFLSPLQL
                     AEKIAHTEGTVSIVFGRESDGLTDEEVAACAQVVSIPTSDAFPSLNLAQAVQIITYTL
                     FEAHLPHPTGLIPITQERAQRASDAAVDALSEIGYFKLDQEELWSRRFLKDVFTRASL
                     SESEVQRMEKLFTKMVRIKIHKKEKPDEQDPPVL"
     gene            1912..2721
                     /locus_tag="PKN79_01030"
     CDS             1912..2721
                     /locus_tag="PKN79_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNZ94382.1"
                     /translation="MSKTHPFFDPMPRIVAHRGDSGSFPENTLPAFESAVSMQVDVIE
                     TDVHISKDGHIVIWHDPTLDRNTDASGRIEDHTLDELKTFDAGYTFTVDGGKTFPFRN
                     KGIRLATLEEALETFPAMRFNVDLKSREPEIVDAFIDLIRQKDAFDRVIGASFHLKNL
                     QALRSKEKRIQTSLTTLEVIPLLTKQKLGLLSSKKQKGLTIFQIPPRQWGIEVVTPRF
                     VEIMHKRGAIIQVWTINEKEEMRRLFAMGVDSIMTDHPQLAIEVAKELGLR"
     gene            complement(2718..3509)
                     /gene="nagB"
                     /locus_tag="PKN79_01035"
     CDS             complement(2718..3509)
                     /gene="nagB"
                     /locus_tag="PKN79_01035"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNZ94383.1"
                     /translation="MRVVIQKDFLAMSKWAAKLIADRINVFHPTEDRPFVLGLPTGST
                     PVGTYKELIALYKEGYVSFKHVITFNMDEYVGLGSDHPQSYHTFMWENFFNHIDIKKE
                     HVHIPNGMAKDLEEESLRYEEAIEVVGVIHLFLGGVGGDGHIAFNEPYSSFDSRTRVM
                     TLTEHTRQANSRFFENKIDLVPRYALTVGVKTVMSAKEVMILISGANKAQALYAAVEG
                     PITQAWPISTLQLHKEAIIVCDEDACGELKVSTYNYFKALEKVMV"
     gene            complement(3559..>4560)
                     /gene="mnmA"
                     /locus_tag="PKN79_01040"
     CDS             complement(3559..>4560)
                     /gene="mnmA"
                     /locus_tag="PKN79_01040"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNZ94384.1"
                     /translation="SHEVIGVTMTIWSNRAHLQRPVDSVSCFAPDKSEDIERIKELCA
                     RLGIEHHTLELSDRFEEEVLSNFKDEYLDGKTPNPCVWCNQRIKFGAMVEYAKKSGLV
                     FDKFATGHYARIVHTGKRWAVGKAKDEKKDQSYFLYRLSQEQLKTILFPLGELAKDEV
                     RVILKEHGHVSDDQEESQDFYDGPYTDLLEVEDKEGAIITRDGRILGTHKGIWHYTIG
                     QRRGLGIAAERPLYVLELRASTNEVVVGFVDETVQHQVEATDVVFGSLGVIEGEVEVE
                     AKIRSVGRPKKAIAHRKENGSIVAFFPEGVNAATPGQSLVLYKDDLILAGGIITHAR"
BASE COUNT         1123 a         1255 c         1179 g         1003 t
ORIGIN      
        1 tcagatttcc ggtgctgggt ccgccgctga aagcttctga tggctttctg gcgggctcgc
       61 tcccgttggt agctttttcc actgctgatc atgatcgcaa aaggcctgag gctcttgata
      121 ttggcaaaaa gaatctgcat cagactggtc agcggcacgg cgatgaacat tccgacgatt
      181 ccccagatat acccccacaa cgaaagggcg acgaggatca tgaacgggga gaggttcagc
      241 tgtccgcctt ggaggcgggg atcgatgata ttgcccagaa tcacctgagt gctgatcgta
      301 agcacggcga ccaggatgat gttcgtccaa tccggagcga actgaaccaa cgacatgaag
      361 atcaccacgg aggtgatcac gatgcttcca atcgaaggaa taaagttgaa gatgaacgcc
      421 aggactcccc acagcgaggc gagatccaac ccggtgagga gggcggaaag gtagaaaaac
      481 aacccggtga agaaactgat gacgatcttt atcaccaggt atttggaaat ctggcgggtg
      541 atccgttcaa acatcaccgc caccttcatc cctttgcctt ggggaaccgc cgccagaagc
      601 ttgggaacca cgctttgccg ctcgagaagc aaaaacagaa agaagatata gaccaggagc
      661 gccaattttg tgattgagaa gaatttgttg gagatctgcg tcaggctgct gatcgccaag
      721 ctcgaccagt tgaccggcag caaggtgaga aaactcgtcg aagggggtac ctcgacgaag
      781 ttgttcacca tatcggtcaa gaggcggtcg agactgatga tcttgtcggt gtaaaagggg
      841 atcaatcgga caagggtgtc aaccgtcttg acaatgaacc atcccgccgc caacagaagg
      901 agaagcatca gagccatcac gaaaatcgtt gaaatgagct tcgggacgtg aagcttgtcc
      961 agccgccgca aaagcgggct gaacatcaga aagaaaaaga atgccaacac aagtggaatc
     1021 gtcacatcct tggagatctt gagcgcagcc aaaaccgcca acgttacgat aatcccgagc
     1081 atgatatgga tatagcgatt acctaccgag gtggattcat tcatgggcat actatagacc
     1141 agcttggata atcctgcaat atctggtaca cctgagctat gacgcacccc agcagactcg
     1201 atcacattca aatcgttctt gtcgacactc aggatggagc aaacatcggt tccgtctgcc
     1261 gggcgatgaa gacgatggga ataacccgcc tagtcatcgt tggcgaacgg gagtacgaag
     1321 aaaaccggat tcgaaccctg gctcttcatg ccggcgacgt ctgggagaat cgagttcaac
     1381 atccaacgct ccaagaagcg ctcaaagaga gcagctacac cgtggctgcc accagaagaa
     1441 gagggaagtt tcgaaagcac acctttctct cccctctgca actcgccgaa aaaattgcgc
     1501 ataccgaggg cacggtctcg atcgtcttcg gacgggagtc ggacgggttg accgatgaag
     1561 aggtcgccgc ctgcgctcag gtggtatcga ttcccaccag tgatgcgttc ccatccctca
     1621 atctcgccca agcggtccaa atcatcacct ataccctctt tgaagcacac cttccccacc
     1681 ccaccggtct tatcccgatc actcaggagc gagcccaaag agcaagcgat gcggcggtgg
     1741 acgccctttc cgagatcggg tatttcaagc ttgatcagga ggagctctgg tcgagacggt
     1801 ttctgaaaga tgtgttcact cgcgcctcgc tcagcgagag tgaagtgcaa cggatggaaa
     1861 aactctttac caagatggtt cggatcaaaa tccataagaa ggagaagccc gatgagcaag
     1921 acccacccgt tctttgaccc gatgccccga atcgtcgccc accggggtga cagcggctcc
     1981 ttccctgaaa acaccctgcc cgcgttcgaa tcggcggtct ccatgcaggt cgatgtgatc
     2041 gaaacggatg ttcacatcag caaggatgga cacatcgtca tctggcatga tccgacgctg
     2101 gatcgaaaca ccgatgcctc cggacgaatc gaggatcaca cccttgacga gttgaaaact
     2161 tttgacgcgg ggtacacctt caccgtcgat ggcggcaaaa catttccgtt caggaacaaa
     2221 ggaattcgcc tcgccaccct cgaagaggcg ttggaaacct tccccgccat gcggttcaac
     2281 gtcgacctga agagcaggga accggagatc gtggatgcct tcatcgatct catcagacaa
     2341 aaggatgcct tcgatcgggt gatcggagct tccttccatt tgaaaaacct tcaagcgctt
     2401 cgaagcaaag agaagcgaat tcaaaccagt ctgacgaccc tggaggtgat tccgctcctt
     2461 accaagcaga agctggggct tctttcatca aagaaacaaa aaggactcac gatcttccag
     2521 attccgcccc gtcaatgggg aatcgaggta gtgactcccc gcttcgtgga gatcatgcac
     2581 aaacggggag cgatcatcca ggtgtggacg atcaacgaga aggaagagat gagacgattg
     2641 ttcgccatgg gggtcgattc gatcatgacc gaccacccgc agctggcgat tgaggttgca
     2701 aaagagctcg gcttgcgtta gaccatcacc ttttcgaggg ctttgaagta attgtacgtt
     2761 gaaaccttca gctcgccgca cgcatcttcg tcacacacga tgatcgcctc tttgtggagc
     2821 tgcagggtcg agatcggcca tgcctgagta atcggccctt caacggcggc atacagcgcc
     2881 tgagccttgt ttgctccgct gatgagaatc atcacctcct tggcgctcat caccgtcttt
     2941 accccgacgg taagggcgta ccggggaacg agatcgattt tattttcaaa aaaccggctg
     3001 ttcgcctgac gggtgtgttc ggtcaacgtc atcacccggg ttcgactgtc aaatgaagag
     3061 tacggctcat tgaacgcgat gtgtccgtca ccgccgactc cgcccaaaaa gaggtgaatc
     3121 acgccaacca cctcgatcgc ttcttcgtag cggagcgatt cttcttccag atcctttgcc
     3181 atcccgttgg ggatgtggac atgctctttt ttaatatcta tgtggttgaa aaaattctcc
     3241 cacatgaagg tgtgatagct ttgcggatgg tcgcttccca aaccgacgta ctcatccatg
     3301 ttgaaggtga ttacgtgctt gaaggagaca tacccttcct tgtacagagc gatcagctcc
     3361 ttatacgttc cgaccggcgt cgatccggta ggaagtccga gcacaaacgg acgatcttcg
     3421 gtgggatgaa aaacgttgat tcgatcggcg ataagcttgg ccgcccattt gctcatggca
     3481 agaaaatctt tttgaataac tacgcgcata ccctgctcct tccacttcat cataatggca
     3541 aaccaaaagg gaggcaactc accgggcatg ggtgatgatg ccgccggcga ggatgaggtc
     3601 atccttgtag agaaccagag attgaccggg ggtcgcggcg ttcacccctt cgggaaaaaa
     3661 ggcgacgatc gacccgtttt ccttccgatg tgcgatcgct ttcttcggtc ttccgacgct
     3721 tctgatcttc gcctccacct cgacctcgcc ttcgatcacc ccaagactcc cgaatacgac
     3781 atcggtcgct tcgacctgat gctgaacggt ctcatcgacg aaaccgacga ccacctcatt
     3841 ggtggaggcc ctcagctcca acacatataa cgggcgctcg gcggcgattc cgaggcctct
     3901 gcgctgaccg atcgtatagt gccaaattcc tttatgcgtt ccaagaatcc gtccgtctcg
     3961 agtgatgatc gccccttcct tgtcctccac ttccaataga tcggtgtaag ggccgtcgta
     4021 gaagtcctgg ctttcttctt gatcatcact tacatgtccg tgttccttca gaatgactcg
     4081 gacttcatcc ttggcaagct ctccaagcgg aaacagaatc gtcttaagct gctcttggct
     4141 gagacgatag aggaaatacg attgatcttt cttctcatcc ttcgctttcc caaccgccca
     4201 ccgcttccct gtatgaacaa tccgggcgta gtgtccggtt gcgaacttgt cgaataccag
     4261 gccgctcttt ttggcgtact cgaccatcgc tccgaatttg atcctttggt tgcaccacac
     4321 gcaggggttc ggagtttttc cgtcaagata ctcatccttg aagttggaaa gaacctcctc
     4381 ctcgaagcgg tcactgagct ccaaggtgtg atgttcgatt cccaagcgag cgcacagctc
     4441 tttgatcctt tcgatatcct cgctcttgtc gggagcgaag caggagactg aatcgaccgg
     4501 tcgctgcaag tgagcgcggt tggaccagat cgtcatcgtg accccgatca cttcatggct
//
LOCUS       DAOPUZ010000030         4536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_19193_length_4536_cov_3.250391, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000030 DAOPUZ010000000
VERSION     DAOPUZ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19193_length_4536_cov_3.250391"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PKN79_01045"
     CDS             complement(<1..124)
                     /locus_tag="PKN79_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HNZ94385.1"
                     /translation="MTSLDTLIKDLSRLPGIGPKSATRLAYHLLKSDPSYNRNLA"
     gene            complement(121..414)
                     /locus_tag="PKN79_01050"
     CDS             complement(121..414)
                     /locus_tag="PKN79_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNZ94386.1"
                     /translation="MNPFELLKNAQAIKEQMARMQDILPAIRATGNAGAGMVTITLNG
                     KFTVVDIAIEEAATEDLETLQLLIQSAFNDASAKLQQKLQDEGLKAVSMMGQS"
     gene            complement(414..2012)
                     /gene="dnaX"
                     /locus_tag="PKN79_01055"
     CDS             complement(414..2012)
                     /gene="dnaX"
                     /locus_tag="PKN79_01055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNZ94387.1"
                     /translation="MSQYEVTATKKRPQEFSTLVGQPFVVSTIEGAIAQGRIAHAYLF
                     SGPRGVGKTSAARILARSLNCDEGPTTKPCGVCSHCIEIKAGNSVDVIEIDGASNTGV
                     NDIRQIKDEVLFPPQTGRYKIYIIDEVHMLSISAFNALLKTLEEPPPYVIFILATTEL
                     QKVPATIRSRCQQFHFQLIGLDLIKESLAQAAVDQGIQADDDALFWIAREATGSMRDA
                     YTLFDQVVAFSAGHITMETIQSKLGLVGFDRIITIATDLLEGNTQTALLAIHSLINAG
                     VSVEQTIKDLTQFYRSLLFMRSQINDEAILGVQSDRVPLALRTAYTTEQLEAALELLL
                     ATYREIRYSLNPRIELELAISRLGELKSLRSNTALVKEIATLREELLSGNVHVTRQVQ
                     APAPSSAPPPKQQELIPAAPPAPPPSRPSRTLTMEDLPELTRRLGDAPMLARVLGTVK
                     ELNQNDRTLTFTFNTAFAATTAKENSERLVKAIEELTGPGVEVYCINGETSKANEESP
                     SGEDPVLRNIATVFRGDVLTRKSQ"
     gene            complement(2069..2167)
                     /gene="ffs"
                     /locus_tag="PKN79_01060"
     ncRNA           complement(2069..2167)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKN79_01060"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2181..2263)
                     /locus_tag="PKN79_01065"
     tRNA            complement(2181..2263)
                     /locus_tag="PKN79_01065"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2227..2229),aa:Ser,seq:gga)
     gene            complement(2283..2367)
                     /locus_tag="PKN79_01070"
     tRNA            complement(2283..2367)
                     /locus_tag="PKN79_01070"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2331..2333),aa:Ser,seq:tga)
     gene            complement(2383..2456)
                     /locus_tag="PKN79_01075"
     tRNA            complement(2383..2456)
                     /locus_tag="PKN79_01075"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2420..2422),aa:Arg,seq:gcg)
     gene            complement(2475..2561)
                     /locus_tag="PKN79_01080"
     tRNA            complement(2475..2561)
                     /locus_tag="PKN79_01080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2525..2527),aa:Ser,seq:gct)
     gene            complement(2596..2683)
                     /locus_tag="PKN79_01085"
     tRNA            complement(2596..2683)
                     /locus_tag="PKN79_01085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2647..2649),aa:Ser,seq:cga)
     gene            2896..3213
                     /locus_tag="PKN79_01090"
     CDS             2896..3213
                     /locus_tag="PKN79_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ94388.1"
                     /translation="MAKRTPVLLPWVERTLIDLGERIRLARLRRNISAQLLADRAGIS
                     RQTLSTLENGSGSVSLATFIQVLFVLGLEKDLEEVAKDDVLGRKLQDLNLPTRRRATK
                     RER"
     gene            3311..>4536
                     /locus_tag="PKN79_01095"
     CDS             3311..>4536
                     /locus_tag="PKN79_01095"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNZ94389.1"
                     /translation="MGKNEAPPSSRKKTKLATFKAGRLAQRALAFTKEVLAQSGPRKS
                     GSLAALEAAELIKSELETTCDTVESQQVPIDSKINTRLFTLVVYAYPAVVLLCLVGLP
                     YLGLLLSALVLWFSFETFGRYKPRYLAPTDMGVNVHGIIEPVKAVESTIIFTAHHDSA
                     PIERFEKKKAMIPFGLVGGAALLSFLQTAVELTQLRLFRPNFPPILLFLLGLGLLGGL
                     YYVRTLLEYYTDEVAPGAGDNLISVGILAALAQYFKAEPLSKTRIILASFDGEEIFLQ
                     GSTAWYEAHKEALEGAVVLNFDSIFSAEHLVFLERDANATLPLSKSLARKCVNIARSM
                     GYDASALSMPILGGATDAASAARIGLEATTLTASSWSDGVYHTKDDTLAAIDPESVER
                     SIGIAIRLARLLEQDSL"
BASE COUNT          943 a         1246 c         1305 g         1042 t
ORIGIN      
        1 cggcaaggtt cctattgtag gaagggtcgc tcttcagcag gtggtaggcg aggcgggtgg
       61 cgctcttcgg tccgatgccc ggcagacggc tgaggtcttt gatgagggtg tccaggctcg
      121 tcatgactgc cccatcatcg agacggcttt caggccttcg tcctgaagct tctgctgaag
      181 ctttgccgat gcatcgttga acgcgctctg aatgagcagc tgcagagttt caagatcctc
      241 ggtggccgct tcttcaatgg cgatatccac aacggtgaac ttgccgttga gcgtgatcgt
      301 gaccattccg gccccggcat tgccggtcgc ccggatcgca ggcaggatat cctgcattct
      361 ggccatctgt tctttaatcg cctgagcgtt cttgagcaat tcaaacgggt tcatcactga
      421 gacttccttg tgagcacatc accgcggaac acggtggcaa tatttcgtaa tacgggatcc
      481 tccccggaag ggctttcttc attggctttc gacgtctccc cattgatgca gtacacttcg
      541 acgccgggcc ccgtcaactc ctcgatcgct ttcaccaggc gttcgctgtt ttccttcgct
      601 gtcgtcgctg cgaatgcggt attgaacgtg aaggtaagcg ttcgatcatt ctggttcagt
      661 tctttcaccg ttcccaagac ccgggcaagc atcggagcgt ctccgaggcg ccgagtgagc
      721 tcgggcaggt cctccatcgt caaggtccgg cttggccggc ttggaggggg agcggggggg
      781 gcggcgggaa taagttcctg ctgcttggga ggcggagcgg aggaaggagc gggagcctgg
      841 acttgtcggg tgacgtgcac atttccggaa agaagctcct cacgcaaggt ggcgatctcc
      901 tttaccaagg cggtgttgga gcgaaggctc ttcagttctc cgaggcggct gatggccagt
      961 tcaagctcga tccgcggatt cagcgaatag cggatttccc ggtatgtggc aagaagcagc
     1021 tccaaggcgg cttccaactg ctccgtcgta taggcggtcc gcaaagcgag gggaactcga
     1081 tcgctttgaa ctcccaaaat cgcttcgtcg ttgatttgtg agcgcatgaa taggaggctt
     1141 cggtagaact gagtgagatc cttgattgtt tgttcgacgg agacccccgc gttgatgagg
     1201 gagtggatgg caagcagggc agtttgggtg tttccttcaa gaagatcggt ggcgatggtg
     1261 atgattctat caaatccgac aagcccgagc ttcgattgga tcgtctccat cgtgatgtgt
     1321 cccgcactga aggcgacgac ctgatcgaaa agggtgtagg cgtcccgcat cgagccggtg
     1381 gcctctcggg cgatccagaa gagggcatcg tcatcggctt ggatgccctg gtcgacagcc
     1441 gcttgggcaa ggctctcctt gatgagatcc agaccgatga gctggaagtg gaactgctgg
     1501 cagcgggagc ggatcgtcgc cggcactttc tgcagctcgg tggtggcgag aatgaagatc
     1561 acgtaggggg gcggttcctc caaggttttg agcagcgcgt tgaacgcgct gatcgagagc
     1621 atatggactt cgtcgatgat gtagatcttg tagcggcccg tctgaggagg aaagagaact
     1681 tcatccttga tctggcggat gtcgttgact ccggtattgc tggctccgtc gatttcaatc
     1741 acatcgacgc tgttgccggc tttgatttcg atacagtggc tgcatacccc gcacggtttg
     1801 gtggtgggac cttcgtcaca gttaagggaa cgggcgagga ttcgggcagc ggaggttttt
     1861 cccactccgc gggggccact gaagagatac gcatgggcga ttcgcccctg ggcgatcgct
     1921 ccttcgatcg tggagacgac gaacggctga ccgacaagag tggaaaactc ctgcggtcgt
     1981 ttcttcgttg cggttacttc atactgtgac atctgccccc caaagttcgt tgacacttta
     2041 ccatagatga aggggtttta atagaaccag ccaacaaatc gcctgcgtct cacaagcggt
     2101 ccacttaccg ctgctccctt ccgggcctga cggggttggg cggatgcctg tagcacagaa
     2161 tctgttggct ggaaaaaaaa cggagagaag gagattcgaa ctcctggtgc ttgcgcacac
     2221 acgctttcca agcgtgcacc ttcgaccact cggacatctc tcccggtgac caataaaacg
     2281 ttcggagaga gagggattcg aacccccgga gacttgcgcc ccaacagttt tcaagactgc
     2341 ctccttcgac cgctcggaca tctctccaaa tattttatgc tatgagacca agaggattcg
     2401 aacctccggc cctcagttcc gcaaactgat gctctatcca gctgagctat ggtctcgcta
     2461 taaaaaaaca aaatcggaga gggggggatt cgaacccccg gacccgttac caggtcaact
     2521 ccttagcagg gagcccgatt cggccactct cgcacctctc cttgaacaaa ccggcttctt
     2581 ggcaactata gccttcggag agagagggat ttgaaccctc ggtactggag accagtacaa
     2641 cggatttcga gtccgccccc ttcggccgct cgggcacctc tccttgtttg gggccctgag
     2701 cagagctcag taaccaatcg gattcaaact atagtcagtg gtatcatatt tgtcaagaag
     2761 ggtttggtcg acacgatcgg gcaatcgtgt tcattcccat caacatgatc ctgtctttca
     2821 taggagggat cttgctttga taacgacgaa tatatttgac atactaacca ttgtatgtat
     2881 aatatattgg atgtaatggc taaacgaaca ccggtattgc tcccatgggt ggaacgaacc
     2941 ctcatcgatc tcggggaacg gatccgtctt gcccggctca gacggaatat cagcgcgcag
     3001 ctgcttgccg accgggcggg catcagcagg cagaccttga gcaccctgga aaacggctcc
     3061 ggctcggtat cgcttgccac gttcatccag gtgctcttcg tactcggact ggaaaaggat
     3121 ctcgaagagg tcgcaaaaga cgatgtgctc ggaagaaagc tccaggactt gaaccttcca
     3181 acccggagac gggcaaccaa gcgcgagcga taggcggtag aaaatccatc gaatcctctg
     3241 taaggatatc gggaatcggg atcgatccgt ttagttgaca gatttccacc acctcgtcat
     3301 catacgaggt atggggaaga acgaggcgcc tccctcttcg aggaagaaaa cgaagcttgc
     3361 gacattcaag gccgggcggc ttgcccagcg cgctttggcg ttcaccaagg aggtgcttgc
     3421 tcaatcgggg ccgcgcaaga gcgggagtct cgctgcgctt gaagctgcgg aattgatcaa
     3481 gagcgagctt gaaaccacct gcgacaccgt tgaaagccaa caggttccga tcgactcgaa
     3541 gatcaacacc cggctgttca ccctcgtcgt gtatgcctat ccggcggtcg ttctgctctg
     3601 tctcgtcggt cttccctatc tcgggcttct gctctccgcc ttggtactct ggttcagctt
     3661 cgagaccttc ggtcgctata aaccccgata tcttgcccca acggacatgg gggtgaacgt
     3721 ccatgggatc attgaacccg tgaaagctgt tgaatcgacg atcatcttca ccgcccacca
     3781 cgacagcgcc ccgatcgagc gcttcgagaa gaagaaagcg atgattccat tcgggctggt
     3841 cggtggagcc gcacttctgt cttttctcca aacggctgtc gaattgactc agcttcgcct
     3901 gtttcgaccc aattttccgc cgattctgct gttccttctc ggccttggac ttcttggcgg
     3961 actctattac gtgcgaacgc tcttggagta ttacaccgat gaggttgccc ccggagcggg
     4021 agacaacctg atctcggtgg ggatacttgc cgcgctcgcc caatatttca aggccgaacc
     4081 tctttccaaa acccggataa tcctcgcttc ctttgacggc gaagagattt ttcttcaggg
     4141 ctcgacggcg tggtatgaag cccacaagga agcgcttgaa ggtgcagtcg tcctgaactt
     4201 tgattcgatc ttcagcgccg aacaccttgt ctttctggaa cgtgatgcga acgcgacgct
     4261 cccgctgtcg aaatcgttag cccgcaaatg cgtcaacatc gcccgctcga tgggctatga
     4321 tgcatcagcg ttgtcgatgc cgattctcgg cggggcgacg gacgccgctt cggcagctcg
     4381 gatcgggctg gaagcgacca ccttgaccgc ctcctcgtgg agtgacggcg tctaccacac
     4441 caaagatgat accttggcgg ccatcgatcc tgagagtgtg gagagatcga tcggcatcgc
     4501 gatccgcctt gcccggctgc tcgagcagga cagcct
//
LOCUS       DAOPUZ010000031         4477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_19529_length_4477_cov_3.503618, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000031 DAOPUZ010000000
VERSION     DAOPUZ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19529_length_4477_cov_3.503618"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKN79_01100"
     CDS             <1..478
                     /locus_tag="PKN79_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94390.1"
                     /translation="LDIDGTKEEAEKKYDSYSNEAKAHVKEGMALTLLSNLTLPKTPN
                     YAEIIPKLIALGAMISDDEELVELLAQMQGFFTQYVETQEDLIQRLKEQFAPHLQQKE
                     AQLRAQYGPNFVLRPEQDPEFMKVLDKQLKTLEEQYTNVLDNAKERMKEILGIEGQ"
     gene            complement(532..1824)
                     /locus_tag="PKN79_01105"
     CDS             complement(532..1824)
                     /locus_tag="PKN79_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNZ94391.1"
                     /translation="MNQDLLKEKALAYHIQDGVPGKVSVVPSKPCQTAEDLSLAYTPG
                     VAKPVLEIAANPEDAYKYTSKGNLVAVISNGTAILGLGDRGALASKPVMEGKGVLFKR
                     FADIDVFDIEIDETDPKKLIEIIKKISVTFGGINLEDIKSPECFEIEQTLIEECDIPV
                     FHDDQHGTAIIATAGLMNACEVVDKKIEEVKVVINGAGAAGISCAKMFLNAGVKRENL
                     ILCDRHGVLRRDRLEGLSPEKQDFAIDTELETLADALKGADVLIGLSVADVVTPEMLL
                     SMDRDPIVFAMANPNPEIAYELAMATRSDLVMATGRSDYPNQINNVLGFPFIFRGAMD
                     VRSRIISEGMKMAAAKALAALTKEPVPDSVAKAYGGKKFTFGREYIVPKPFDPRVIEW
                     EAVAVAKAACEEGLAAAPITDWDAYRESLKQRMAHWWA"
     gene            complement(1838..2980)
                     /locus_tag="PKN79_01110"
     CDS             complement(1838..2980)
                     /locus_tag="PKN79_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNZ94392.1"
                     /translation="MKYLDEYAKQYEDCAWLPFEELEAFMKEALIHAGVPREDAEIIS
                     DVLIESDKRGIDSHGIGRLKPIYIDRIDDGIMLPTTRIEVLKDKAAAAVLDGHNGMGH
                     VVSHKAMSMAFEKAQTYGIGMVTVRNSNHYGISGYYATMATRKGLLGITGTNARPSIA
                     PTGGVENMLGTNPLVFGMPTDEEFPFVLDCATSVSQRGKIEVYGRAGKELPEGWVIDN
                     EGRSRTDTLQVLKDLTTGKAALTPLGGIGEVTAGYKGYGYATVVEILSAALQAGSFLK
                     ALNGFDENGNQIPYPLGHFFIAIDPEFFMGLETFKKITGQILRDLRASKKAPGEDYIF
                     TAGEKEYLAYQYRKEHGCPVPPSLQKVMITLRDRWNMDMSWDFEKK"
     gene            complement(2977..3768)
                     /locus_tag="PKN79_01115"
     CDS             complement(2977..3768)
                     /locus_tag="PKN79_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNZ94393.1"
                     /translation="MANIPASRQQYILSRIQDEGTVNVNQLSDALDVSELTIRRDLDQ
                     LAKRGLVERTHGGASLRRNLPVEPDYLQKASEFPLEKSMIGQGVLDLVEDGDTLYINS
                     GTTTLEAIRALVASGKRLTIVTNNIDAAYVCRESQAIRLVLAGGVYRTRSHSVSGSLS
                     SLIVEQIWANKAIIGVDGFSVQAGLTTPVLEEAETTRAMIKHTAGKVIVVAAANKIGV
                     VSNYKTVSLEDVDILVTDEKGAELVSQMEISDDLSIVVAPTRRSE"
     gene            4054..4365
                     /locus_tag="PKN79_01120"
     CDS             4054..4365
                     /locus_tag="PKN79_01120"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNZ94394.1"
                     /translation="MADNTVPVIVENREIQDMIYTIRGRQVMLDSDLAKLYQVETRIF
                     NQTVKRNIDRFPERFRFQLTEKEYENLRSQFVISSEGAKHGGRRYMPYVFCLRIDGNS
                     A"
BASE COUNT          942 a         1262 c         1234 g         1039 t
ORIGIN      
        1 gctcgatatc gacggaacga aggaagaggc agagaagaag tacgattcct actccaatga
       61 ggccaaggcc catgtgaaag agggaatggc gctcaccctt ctttcgaacc tgaccctgcc
      121 gaaaaccccc aactacgccg agatcatccc caagctgatc gccctcgggg cgatgatcag
      181 cgacgatgaa gagcttgtag aactcctcgc tcaaatgcag gggttcttca cccaatacgt
      241 cgaaacccag gaggatctca tccagcggct gaaggagcag ttcgctcccc acctccagca
      301 gaaggaagcg cagctcaggg cccagtacgg cccgaacttc gtccttcgcc cggagcagga
      361 tcccgagttc atgaaggttc tggacaaaca gctcaaaacc cttgaagaac aatacaccaa
      421 cgtcctggac aacgcgaagg agcggatgaa ggagatccta ggcatcgaag gacaatagaa
      481 cggatcaggc tgcccggagg cagcctgtat cgtatcaccc ttcttgattg cttacgccca
      541 ccagtgagcc atgcgctgtt tcagcgactc gcggtatgca tcccaatcgg tgatcggagc
      601 tgcggccagg ccttcctcgc aggcggcttt cgccacggcg accgcctccc attcgatgac
      661 ccgggggtcg aacggcttgg gcacgatata ctcccgtccg aaggtgaact tcttcccacc
      721 gtacgctttt gcaaccgaat cgggaaccgg ctccttcgtc aaggcggcga gcgctttggc
      781 ggcggccatc ttcatccctt cgctaatgat ccgggagcgc acgtccatcg cgccgcggaa
      841 gatgaagggg aagcccagga cgttgttgat ctggttcggg tagtcgctac gccccgtggc
      901 catgacgagg tcgcttcgag tcgccatcgc aagctcgtag gcgatctccg gattggggtt
      961 cgccatggcg aagacgatgg gatcgcggtc catcgaaagc agcatttcgg gggtcaccac
     1021 gtcggcgacg gaaaggccga tgaggacatc cgcacccttg agggcgtcgg cgagggtttc
     1081 cagctcggta tcgatcgcaa agtcctgctt ctccggggag agcccttcca agcggtcccg
     1141 gcgaagaacg ccgtggcggt cgcagagaat caggttctct cgcttcactc ccgcgttcag
     1201 gaacatcttc gcacagctga tgccggcggc tcccgcgccg ttgatgacga ccttgacttc
     1261 ctcgatcttc ttgtccacca cctcgcaggc gttcatcaga cccgctgtgg cgatgatcgc
     1321 cgttccatgc tggtcgtcgt ggaagacggg aatgtcacac tcttcgatga gagtctgttc
     1381 gatttcgaag cattccggac tcttgatatc ctcaaggttg atgccgccga acgtcacgct
     1441 gatcttcttg atgatctcga tcagcttctt cggatcggtt tcgtcgattt cgatgtcgaa
     1501 cacgtcgatg tcggcgaagc gtttgaagag aactcccttc ccttccatga cgggcttgct
     1561 ggccaatgca ccccggtccc ccagaccgag gatcgccgtt ccgttggaga tgacggccac
     1621 cagatttccc tttgacgtgt acttgtaggc atcctcggga ttcgcggcga tctccagaac
     1681 cggctttgcc accccggggg tgtaggcaag gctcagatcc tcggcggtct ggcagggctt
     1741 gctcggaacg accgaaacct ttccgggaac tccgtcctgg atgtgatagg ccaacgcctt
     1801 ctctttcagc agatcttgat tcatcttatt cttctcctca ctttttctca aaatcccaac
     1861 tcatgtccat attccagcgg tcacgcaggg tgatcatcac cttttgcagg gacggaggaa
     1921 ccggacatcc atgttctttg cggtactggt acgcaagata ttctttctct cccgcggtga
     1981 agatatagtc ttcgccggga gcttttttgc tggcccgaag atcgcggaga atctgaccgg
     2041 tgatcttctt gaaggtttca aggcccatga agaactcggg atcgatcgcg atgaagaagt
     2101 gcccgagcgg ataaggaatc tggttgccgt tctcgtcgaa gccgttcaaa gctttcagga
     2161 atgaacccgc ctgcagtgcg gcggaaagga tctcgacgac cgtcgcatac ccgtagcctt
     2221 tgtatccggc ggtcacttcg ccgattcccc cgagcggggt cagggccgcc ttgccggtgg
     2281 tcaggtcctt gagcacctga agggtgtcgg tgcggcttct gccttcgttg tcgatgaccc
     2341 atccttcggg cagctctttt ccggcgcggc cgtacacttc gatcttgccg cgctgggaga
     2401 cgctggtggc gcagtccaga acgaacggga actcttcatc ggtgggcatt ccgaagacca
     2461 gcgggttggt cccgagcatg ttttcgacgc cgccggtcgg ggcgatcgag ggccgggcgt
     2521 tcgtgccggt gattccgagc aatcccttcc tggtcgccat cgtcgcatag tagccgctga
     2581 tgccgtagtg gttggagttg cggaccgtta ccatgccgat gccgtacgtt tgggctttct
     2641 cgaacgccat gctcatcgct ttgtgactga ccacgtggcc catgccgttg tgtccgtcga
     2701 ggacggcggc ggcggccttg tccttaagca cttcgatcct ggtggtgggc agcatgatcc
     2761 cgtcgtcgat ccggtcgatg tagatcggct tcaggcggcc gattccgtgg ctgtcgattc
     2821 cccgcttgtc gctttcgatc aggacgtcgc tgatgatctc ggcgtcttcc ctgggaaccc
     2881 ccgcgtggat caacgcctct ttcatgaagg cttccagctc ttcaaacggc agccatgcac
     2941 agtcctcata ctgctttgca tattcatcca agtacttcat tcgctcctcc tcgtcggtgc
     3001 tactactatt gaaagatcgt cactgatctc catttgactt accagttcgg cgcccttttc
     3061 atcggtcacc agaatgtcca catcctcaag cgagacggtc ttgtagttgg ataccacccc
     3121 gatcttgttc gccgcggcga ccacgatgac ttttccggcg gtgtgcttga tcatcgcccg
     3181 ggtggtttcc gcttcctcca agaccggcgt ggtcaatccc gcctggacgg agaatccgtc
     3241 aaccccgatg atcgccttgt ttgcccagat ctgctcgacg atcagggaag agagggagcc
     3301 ggagacggaa tggctccgcg tccggtacac tccgccggcc agcaccagac ggatcgcctg
     3361 gctttccctg cacacatacg ccgcatcgat gttgttcgtc acgatcgtca gccgttttcc
     3421 ggaagcgacc agcgcccgga tcgcttccaa ggtggtggtt ccgctgttga tatataaggt
     3481 atcgccatcc tcgaccaggt cgaggacgcc ctgtccgatc atgcttttct caaggggaaa
     3541 ttccgatgct ttttgaaggt aatcaggttc gacgggcaga ttccgcctga ggctcgcccc
     3601 gccgtgggtc cgttcgacga ggccccgctt ggcaagctgg tccagatccc ggcggatcgt
     3661 cagctcgctg acgtccaagg catcgctcaa ctgattgaca ttcaccgtcc cttcgtcctg
     3721 aatccgggac agaatgtact gttggcgact tgccggaatg tttgccatga atacttcctg
     3781 attgtttttg aacttattta tacaatacat tcaaaaacat tcacatgtca atacaccatg
     3841 catctgtcgc cttctgacga tctgtttgcg tccctacttg atgatgtcca caaagcacga
     3901 aatccgaaca tcggtaaact tcaagcaaac aagggattgt caccacataa tcaggtaaga
     3961 tgatttttat ttgcgtatag acggaaacag tgcatgatgc catatggaag tggttgcaag
     4021 ttgcggccgg ttatggagaa tccggaggta acgatggcag acaacacagt acctgtgata
     4081 gtagaaaata gagaaattca ggatatgata tataccatta ggggtaggca ggtcatgctg
     4141 gatagtgact tagctaagtt gtatcaagtt gaaacaagaa tttttaatca gactgtgaaa
     4201 agaaatatag acaggtttcc tgaacgattc aggttccaat tgacggagaa ggagtatgaa
     4261 aacttgagat cacaatttgt gatctcaagt gagggcgcca aacatggtgg tcgaagatac
     4321 atgccatatg tcttttgctt gcgtatagac ggaaacagtg catgatgcca tatggaagtg
     4381 gtcgcacatt gcggtcggtt atgcggaggt aacgatggca gacaacacag tacctgtgat
     4441 agtagaggac agagaaattc aaagcatgat ctatacc
//
LOCUS       DAOPUZ010000032         4440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_19763_length_4440_cov_3.462714, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000032 DAOPUZ010000000
VERSION     DAOPUZ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19763_length_4440_cov_3.462714"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /gene="lepA"
                     /locus_tag="PKN79_01125"
     CDS             <1..1099
                     /gene="lepA"
                     /locus_tag="PKN79_01125"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNZ94395.1"
                     /translation="EVGRFLLSRQKRDKLSAGDVGYVITGVKTISDVRVGDTITSAAK
                     PAKRPLPGFKDVKPVVFSSIYPVDTNDYEELVDALDRLKLNDASLVYEKDSSAALGFG
                     FRCGFLGMLHLEVVQERIEREFGLSIVFTSPSVQYHVHMKDGSMLSVDNPLEYPEPMK
                     IDYAEEPYIQANIITPVSYLGPIINLCIDRRGTQVGMNYLDAKRVELIYEMPLAEVLF
                     EFYDRLKSVSRGYASFDYDVIGYRRTDLVRLDILVNGEPVDALSQLVFRGNSQKRGRM
                     VCERLRKEIPRQQFKIAIQAAIGGTIISRETISAFRKDVTAKCYGGDISRKRKLLEKQ
                     KEGKKRMKMVGNVEIPQSAFLAVLKSDEDSN"
     gene            1115..2149
                     /locus_tag="PKN79_01130"
     CDS             1115..2149
                     /locus_tag="PKN79_01130"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNZ94396.1"
                     /translation="MVHVQKIAESPKGEDIFKVTLESGPYTVQILNLGATIQSLIVPD
                     RDGKKRDIVLGFEDPFEYLGHSAYFGQVVGRFANRIKDATFTLNDRTYKLDANDGKNS
                     LHSGSANWGWRLWAIEPFEWEEKPGVVLSITSQAGEGGFPGSVTATVSYLLDGESGQL
                     RLEYEATASEETPMNLTNHSYFNLAGAGSGTILHHDVQLFCDRYVEVDGHAIPTGKVL
                     GVEQTPFDFRKSKAVGLDIETAGGYDHCFILSDERSELRPAATVYDPGSGRMMEIFTT
                     LPSLQFYTSNFLDGSTVGKGGVPYQKHAGMCFETQYYPDSPNQTHFPNAIFGPDRPYR
                     HTTVFAFSTK"
     gene            2157..2783
                     /gene="udk"
                     /locus_tag="PKN79_01135"
     CDS             2157..2783
                     /gene="udk"
                     /locus_tag="PKN79_01135"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNZ94397.1"
                     /translation="MKKVKIIGITGGSGSGKSTIVRKISEVCSDFVFIPQDNYYKSAA
                     YISNENITAFNFDHPDAFDMDLLYRHLSDLKAYLPIKMPLYDFVNHRRRDETVHVDPR
                     PLVIIEGLMVLHDPEIRALLDLKLYVDTPDDIRFIRRLKRDISERGRTLESVCAQYLE
                     VVRPGHFNFIEPTKVYADLIIPEGGYNESALSVLIPFVKGITSADDDV"
     gene            complement(2758..3036)
                     /gene="yidD"
                     /locus_tag="PKN79_01140"
     CDS             complement(2758..3036)
                     /gene="yidD"
                     /locus_tag="PKN79_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNZ94398.1"
                     /translation="MKRFLRQLFLIPVYLYKGLLSPFFGSSCIYHPSCSTYMVEAVLR
                     HGIIKGSIMGIARILRCSRWFLGGEDPVPEDWSFKAIKDEFTRHRRRS"
     gene            3133..3303
                     /locus_tag="PKN79_01145"
     CDS             3133..3303
                     /locus_tag="PKN79_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94399.1"
                     /translation="MAGNVSKNARREGTKVMTSRWGGAIKMKSVFENGKLRHMAYCEK
                     SGNTARKPKDLM"
     gene            3779..>4440
                     /locus_tag="PKN79_01150"
     CDS             3779..>4440
                     /locus_tag="PKN79_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HNZ94400.1"
                     /translation="MVVAPPVVEEVVYPYGVRPIVKNADGNEVFDLFIVHTNDVKGNI
                     YAENGGVGIAKLSTALKAGREITDNWLLLNTGYVGEIPAEAALTAAWIVDELGYDAYL
                     PQAIQIELGIEGTQKAIPLAANVLDAEEYLLFQPYQVYDFNGFTVGVVGIVAPKPVSG
                     VSFDADVILDNAQWAVDIAKDYVDYLVVLTDLGEGVFSSSDLAENIQGIDLIVDGSGS
                     ETA"
BASE COUNT         1071 a         1118 c         1266 g          985 t
ORIGIN      
        1 agaggtgggc cgctttctcc tgagccgcca gaagcgcgac aagctctccg ccggggatgt
       61 cgggtacgtg attaccgggg tgaagacgat cagtgatgtc cgggtcggcg atacgatcac
      121 gtcggctgca aaaccggcga agcggccgct gccggggttc aaggatgtca aaccggtggt
      181 gttcagctca atctatcccg tcgatacgaa cgactatgag gagctggtcg atgcactcga
      241 ccgcctcaag ctgaacgatg cttccctggt gtacgaaaaa gactcctccg ctgcgttggg
      301 tttcggcttc cggtgcggat ttctggggat gcttcacctc gaagtggtcc aggaacggat
      361 cgagcgggag ttcggtctct ccatcgtatt caccagtccc tcggtccaat accatgttca
      421 tatgaaggac gggtcgatgc tctcggtcga caacccgttg gaataccccg aaccgatgaa
      481 aatcgattac gccgaagagc cgtacattca agcgaacatc atcactcctg tttcatacct
      541 cggaccgatc atcaacctgt gcatcgatcg aaggggaacg caggtgggga tgaactacct
      601 tgatgcaaaa cgggttgaac tgatctacga gatgccgctt gccgaggtcc tttttgaatt
      661 ctacgatcgc ctcaaatccg tcagccgcgg ctatgcctcc tttgactacg atgtcatcgg
      721 gtaccggcga accgatctgg ttcgattgga cattctggtg aacggcgagc cggttgatgc
      781 gctcagccag ctcgtcttcc gcggcaacag ccaaaaacgg ggacggatgg tgtgcgagcg
      841 cctgcgcaag gagattcccc gtcagcaatt taaaattgcc atccaggcgg caatcggtgg
      901 tactatcatc agcagggaga cgatcagtgc gttccgcaag gatgtgacgg cgaagtgcta
      961 tggtggtgac atctctcgca agcgcaagct gctggaaaaa cagaaggaag gcaagaagcg
     1021 gatgaagatg gtcggaaacg ttgaaattcc acaatcagcg ttccttgccg tgttaaaaag
     1081 tgatgaggat tccaattgaa gtcgaggagg agccatggta cacgttcaaa agatcgcaga
     1141 aagtccgaag ggtgaggaca tcttcaaggt aaccttggag tcaggtccgt acaccgttca
     1201 gattctcaat ctgggagcga cgatccaatc cctgatcgtt cccgaccggg acggaaagaa
     1261 gcgggatatc gtcttggggt ttgaagatcc ttttgaatat ctcggccatt cggcgtattt
     1321 cgggcaagtc gtcggtcgtt tcgccaaccg gatcaaggat gcgacgttca ccctcaatga
     1381 ccgcacctac aagcttgacg ccaacgacgg aaagaactcg ctgcacagcg gttccgcaaa
     1441 ctggggttgg cggctctggg cgatcgagcc ctttgaatgg gaagagaagc ccggcgttgt
     1501 tctttctatc acttcccagg cgggtgaagg agggtttccc ggcagcgtaa cggcaacggt
     1561 aagctacctg ctggacggag agagcggaca gctgcgactt gagtatgaag cgacggcaag
     1621 cgaagagacc ccgatgaacc tcacgaacca cagctacttc aacctcgcag gcgccgggag
     1681 cggtacgatt ctgcatcatg atgtccaact gttctgcgac cggtatgtcg aagtcgatgg
     1741 ccatgcgatt cccaccggca aggttcttgg agtcgaacag acgccttttg atttcaggaa
     1801 atccaaagcg gtcggcttgg acattgagac ggcgggcgga tacgaccact gcttcatttt
     1861 gagcgatgag cggtccgagc tccgcccggc ggcgacggtg tatgatccgg gcagcggccg
     1921 gatgatggag atcttcacca cgttgccgtc tttgcagttc tatacgagca acttccttga
     1981 cggaagtacg gtcggcaagg ggggcgtgcc ctatcaaaaa catgcaggca tgtgcttcga
     2041 aacgcagtac tatccggatt cacccaacca gacgcatttc cccaacgcga tcttcggtcc
     2101 cgacagaccc tatcgccata cgacggtatt cgcattttca acaaagtgag agcgagatga
     2161 aaaaggttaa aatcatcgga atcaccggag gatcggggtc ggggaaatcg acgatcgtgc
     2221 ggaagatcag cgaggtctgt tccgacttcg tcttcattcc tcaggacaac tactataaat
     2281 ccgcagccta tatttcaaat gagaatatca ctgcgttcaa tttcgatcat cccgatgcgt
     2341 tcgacatgga tctactttac aggcacctct ccgacttgaa ggcatatctg cccatcaaga
     2401 tgcctttgta tgattttgtg aaccaccgtc ggcgggatga aacggtgcat gtcgatcctc
     2461 ggccattggt gattattgaa gggttgatgg tccttcacga tccggagatt cgggcgctgt
     2521 tggatttgaa gctgtacgtg gatactccgg atgacattcg cttcatccgc cgcctgaagc
     2581 gggacatctc cgagcgggga aggactttgg agagcgtgtg cgcccagtat ctggaggtcg
     2641 tccggccggg acacttcaac ttcatcgaac cgacgaaagt gtacgccgat ctgatcattc
     2701 ccgaaggggg atacaacgaa agcgccttga gcgtgctgat tccattcgtc aaggggatca
     2761 cgagcgccga cgatgacgtg tgaactcatc cttgatcgct ttaaatgacc aatcttcggg
     2821 tacgggatct tctccaccga gaaaccaccg cgagcagcgg aggattcggg cgattcccat
     2881 gatcgatcct tttatgattc catggcgcag caccgcctcg accatatagg tggagcaact
     2941 cggatgatag atgcaggagg agccgaagaa cggggagaga agtcctttgt acaaatagac
     3001 ggggatgaga aagagttggc ggagaaaacg cttcatgaac cgtattgtat gcaaggcggg
     3061 gggatttgac tagcatggga aaaacgagta cagtaagagt gttgaattga agtttgattg
     3121 gaggaatggc atatggcagg aaacgtatca aagaacgccc ggcgtgaagg aaccaaagtg
     3181 atgaccagcc gctggggcgg agcaattaag atgaaatcgg tctttgaaaa cggcaagttg
     3241 cggcatatgg cgtactgtga gaaatccggc aacaccgccc gcaagccgaa agatttgatg
     3301 taattcattg cggaattgga aggaacccgt agaaaacgat ctatgggttt tttgttatcc
     3361 gagcttcgcc aagtcactaa aaattcccgt gataaaggca cgtttacccg atttttggtc
     3421 cattatagaa agatttgagg ggatatcgtt acacaaaaag tgatgtttac ggtatcgcac
     3481 ttcaatttgg tgtgatatag ttactgtacg catttatgca acgacatgca aattcaagag
     3541 gaggaaaacc gtgaaacgtt tttcaagcac aattttgacg gctttgctgg tcacattgtt
     3601 ggcattttca ctcgcaagct gtgcgacagc gcgagtcgag ccaattcctc cagcagtggt
     3661 tgagcccgtt ccggctccgg caccaccacc agcagtccca gcaccggaac ccgcccctgt
     3721 tgtagaggtg ccgatggttg ttgagcctgt taagccagtg gctgttgaac cggctcctgt
     3781 ggtagtcgct ccccccgtag tggaggaggt cgtctatccc tatggagtcc gtccaatcgt
     3841 taagaacgct gatggaaatg aagtgttcga tcttttcatc gttcacacca atgacgtgaa
     3901 gggcaatatc tatgctgaga acggcggagt cggaatcgcc aagctctcta ccgccctgaa
     3961 ggccggtcgt gagattaccg acaactggtt gttgctcaac accggctatg tcggggagat
     4021 tcccgccgaa gcggccctta ccgcagcatg gatcgtcgat gagctgggct atgatgcata
     4081 tctgccgcaa gcgattcaga tcgagctcgg gattgaagga acccaaaagg cgatccccct
     4141 cgccgcgaac gtgttggatg ccgaagagta cctgctcttc cagccctacc aagtctatga
     4201 tttcaacggc ttcaccgttg gggttgtcgg catcgtggcg ccgaaaccgg taagcggagt
     4261 aagctttgac gccgatgtca tcctcgacaa cgcccagtgg gccgtcgaca ttgctaagga
     4321 ttacgtggac tacctcgtcg tccttaccga tctcggtgaa ggcgtgttca gttcgtccga
     4381 tctggcagag aacattcaag gtatcgacct gatcgtcgat ggcagcggtt cggagacagc
//
LOCUS       DAOPUZ010000033         4369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_20215_length_4369_cov_3.136069, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000033 DAOPUZ010000000
VERSION     DAOPUZ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20215_length_4369_cov_3.136069"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1550)
                     /locus_tag="PKN79_01155"
     CDS             complement(12..1550)
                     /locus_tag="PKN79_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ94401.1"
                     /translation="MENKNVLFKIRGLKQHFPLKKRGLVVRAVDGVDVDILRGETLGL
                     VGESGCGKSTFGRVLLQLYRQTDGKTLYYGRSLEDLAPAYVKNTYTHLAKHKNQLQIL
                     QKAFEDAAAEYERLKDDESQKAEVVYTALNKSNEAEKAYRNAYLDIVQLIGGLFVVED
                     VEEIKALLLDTYEASFERHRVDEQLKDIALKIAETQRSHNRKPASSKEKRIASLKEIE
                     ATLKNALETAAANIVEANKKLEQKRKEYRHVPGFDEHEGYRNEGIDLSRLTFNEMRLL
                     RNDLQLIFQDPYSSLNPRMTVGQIIGEGLLAHRFFKKDGPEMQEYVMKVMDECGLAPY
                     MIHRYPHQFSGGQRQRIGIARSLAMNPQFVVCDEAVSALDVSIQAQIINLLRDLKEKS
                     NLTYLFISHDLAVVKYISDRIGVMYLGNIVELAPAESIFTNMMHPYTEALLAAIPTTD
                     RGAKKEPIILEGDIPSPINPPSGCKFHTRCRYKTEICTMVEPEWRELGKGHFVACHHP
                     LTEK"
     gene            complement(1540..2505)
                     /locus_tag="PKN79_01160"
     CDS             complement(1540..2505)
                     /locus_tag="PKN79_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ94402.1"
                     /translation="MHTYFTTKRGIVKAVNGVSYKVEAGKTLGVVGESGCGKSVSAMS
                     ILKLLDGNGYIAGGSITFKGQELTTLSEKEMYEVRGNAISVIFQEPMTSLNPVFTVER
                     QISEAFMVHQNMDKKTAARESVRMLTDVQIPNPEEVVKQYPHQLSGGMRQRVMIAMAL
                     ACKPDLLIADEPTTALDVTIQAQILRLMNNLQKEKRTAILFITHDLGVINQMADDVAV
                     MYSGEVVEMAPVDVIFGESKHSHPYTEGLMLSVPRLDTASQSKLETITGSVPHPLDLP
                     KGCFFAPRCKYATKKCQTEHPELVSVGKQHTVRCFYPEKGARSGK"
     gene            complement(2543..4339)
                     /locus_tag="PKN79_01165"
     CDS             complement(2543..4339)
                     /locus_tag="PKN79_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ94403.1"
                     /translation="MAKKPLGSTFTDVLKTFFLGRQRATLSILEEEQVQSPYRTMLRN
                     FREKWSAMLGVYMFLAIFLAVFILPIFFPLDTQFQDPTQQNTRPTFSYMKYDKALQGN
                     VASIDGGSVFGVGVDRDGKLYQWGTLSAKLQKMPPADKRYVQASAGLDHAVALDADGK
                     VYTWGNDRFTLNMIPPELSSRKVKQVIAGHQISIALTEGGEIYVWGNSNLISIPVPKD
                     KQGTFTKVVANTTLAAAITGNNELVILSSRETPFSYIPSEIQGRVRDVDLTERSAAAV
                     TTDGRIYVWGGDDYGVMDIPAHAQGRAIAVSAGRGHFVALLDDGSVVAWGRNNYKQTN
                     VPKLKNIVSIQSSYYQNYAVDKNGRIHTWGLKGYLMGTDQYGRDLFVRLLTGGRVTLT
                     IGALAVIISAIIGIIIGSFSGYYGGKTDMFLMRLAEVVNAIPFLPLAMILSAIIGSRI
                     SETGRISMIMVILGLLNWPSLARLVRGQILAAREQEFVTAAKAMGITEMKIIFKHILP
                     NVITVVLVNLTLSFALSMLYESSLSYLGFGVNEPNATWGNMLYGCNDSTVMSLYWWRW
                     VFPAIALSLCTVSINMAGDGMRDAIDPKSIGR"
BASE COUNT          866 a         1211 c         1261 g         1031 t
ORIGIN      
        1 gaaagagcat cctacttctc cgtcagtggg tggtggcagg caacaaagtg ccccttgccc
       61 aactcgcgcc attcgggctc caccatcgtg caaatctcgg tcttgtagcg gcagcgggta
      121 tggaacttgc acccggacgg agggttgatc ggggaaggaa tatcaccttc gaggatgatc
      181 ggttccttct tcgctccccg atcggtcgtc ggtatggcgg caagcagtgc ttccgtatag
      241 gggtgcatca tattggtgaa gatgctttct gcgggagcga gctcgacgat gttcccgagg
      301 tacatcacgc cgatccggtc ggagatgtac ttcaccaccg ccagatcgtg gctgatgaag
      361 aggtaggtga ggttggattt ctccttcaag tcccgaagga ggttgatgat ctgggcctga
      421 atcgaaacgt ccagggcgct taccgcctcg tcgcagacga cgaactgggg attcatcgcg
      481 agcgatcggg cgattccgat ccgctggcgc tgtccgccgg agaactggtg gggatagcgg
      541 tggatcatgt acggcgcaag gccgcactcg tccatcacct tcatcacata ctcctgcatc
      601 tccggaccgt ctttcttgaa gaagcggtgg gccaaaagtc cttcgccgat gatctgaccg
      661 acggtcattc gagggttcag ggaggagtag ggatcctgaa agatcagctg cagatcgttg
      721 cgcagaaggc gcatctcgtt gaaggtgagc cgactcaggt cgattccctc attccggtag
      781 ccctcatgct cgtcgaaccc cgggacatgc ctgtattcct tccgcttttg ttcgagtttt
      841 ttgttagcct cgacaatgtt cgcggctgca gtttcaagag cgttcttcag ggtcgcttcg
      901 atctctttga gggaagcgat ccgcttctcc ttggagctcg ccggcttcct gttgtgtgaa
      961 cgctgagttt cagcgatctt gagggcgatg tctttgagct gctcatcgac ccggtggcgc
     1021 tcgaagcttg cttcgtacgt atccaagagg agagccttga tctcttcaac gtcttccacg
     1081 acgaagagcc ccccgatcag ttgaacgatg tccaaatagg cgttccggta cgctttctcc
     1141 gcttcgttcg atttgttcaa tgcggtatag acgacctcgg ctttctgaga ttcatcatcc
     1201 ttcagccgtt cgtattcggc ggctgcatct tcgaaagcct tctgcaatat ctgaagctga
     1261 ttcttgtgtt tcgccaaatg agtgtacgtg tttttcacat aggcgggagc aagatcttca
     1321 aggctccttc catagtacag ggtctttccg tcggtttgcc ggtagagttg caaaagaacc
     1381 cggccaaagg tcgactttcc acagccgctt tcgccgacca ggccgagggt ttccccccga
     1441 aggatgtcga catcgactcc atcgacggcc cgaacgacaa gcccccgctt tttcagcggg
     1501 aaatgctgct tcaatccgcg tatcttgaac agaacattct tattttccac ttcgtgcccc
     1561 cttttccgga tagaagcagc gaacggtgtg ctgctttcct acactgacca actccgggtg
     1621 ttccgtttgg cacttctttg tcgcgtactt gcaccgcggg gcgaagaagc agcctttggg
     1681 aaggtcgagg gggtgtggga cgcttccggt gatcgtttcc agcttcgatt ggctggcggt
     1741 atcgaggcgg gggacggaga gcatcaatcc ttccgtgtag gggtgggaat gcttgctttc
     1801 tccgaagatg acgtcaacgg gtgccatctc gactacttca ccgctgtaca tcaccgccac
     1861 gtcgtccgcc atctggttga tgactccgag atcgtgggtg atgaagagaa tcgccgttct
     1921 cttctctttt tgaaggttgt tcatcagtcg gagaatctgg gcctggatcg ttacgtccag
     1981 cgcggtggtc ggttcatcgg cgatcagcag atccggtttg cacgccaacg ccatcgcgat
     2041 catcacccgc tggcgcatcc ctccactgag ctggtgggga tattgcttga ccacctcctc
     2101 cgggttgggg atctggacgt cggtgagcat ccgcaccgac tcccgggcgg cggtcttctt
     2161 gtccatattc tggtggacca tgaacgcttc gctgatctgc cgctcaacgg tgaagacggg
     2221 gttcaggctc gtcatcggtt cctggaagat gacgctgatc gcattgccgc gaacctcgta
     2281 catctctttt tcactgaggg tggtaagctc ctgacctttg aaggtgatcg agccgccggc
     2341 gatatatcca ttgccatcca agagcttgag gatcgacatc gccgaaacgg atttaccgca
     2401 tccgctttcg ccgacgaccc ccaaggtctt tcccgcttcc actttgtagg aaactccgtt
     2461 gaccgctttg acgattccac gtttcgtggt gaaatatgtg tgcaggtcgt ttaattcaag
     2521 taaggtcgac acagcagtac tcctatctac cgatcgactt cgggtcgatc gcgtcgcgca
     2581 tgccgtctcc ggccatattg atactgacgg tacacaggct caaggcgatt gccgggaaca
     2641 cccagcgcca ccagtacagc gacatgaccg tcgagtcgtt gcatccatag agcatgttgc
     2701 cccacgtcgc attcggttcg ttcacgccga aaccgaggta gctcaatgaa gactcataga
     2761 gcatggacag ggcgaagctc agggtgaggt tgacaagaac cacggtaatg acgttgggca
     2821 ggatatgctt gaagatgatc ttcatctccg tgatgcccat cgccttcgcc gcggtgacga
     2881 actcctgttc ccgggctgcg aggatctgtc cgcgaaccag ccgggcgaga gatggccagt
     2941 tcaaaaggcc cagaatcacc atgatcatcg agatccttcc cgtctcgctg atcctgctgc
     3001 cgataatcgc cgagaggatc atcgccagag ggaggaaggg aatcgcgttg acgacctcgg
     3061 caaggcgcat caggaacata tcggttttcc ccccgtagta cccggagaat gatccgatga
     3121 tgattccgat gattgcgctg atgatgacag cgagcgcccc gatcgtcaag gtcacccgac
     3181 cgccggtcaa aaggcggacg aagaggtcgc ggccatactg gtcggttccc atcagatagc
     3241 ctttcagacc ccaggtgtgg attcggccgt tcttgtcgac cgcgtagttc tgatagtacg
     3301 aggattggat cgatacaata ttcttcaact tcggaacgtt cgtctgcttg tagttgtttc
     3361 tgccccacgc gacgacgctt ccgtcatcca gaagggcgac gaagtgtccc cgtccggcgc
     3421 tgactgcgat cgcccggcct tgggcatggg cggggatgtc catcacaccg tagtcgtcac
     3481 cgccccagac gtaaatccgg ccgtcggtgg taaccgcggc ggcggagcgc tcggtgaggt
     3541 cgacatcgcg gacgcggcct tgaatctcgg aagggatata ggagaagggt gtctcccggg
     3601 aggagagaat gaccagttcg ttgttgccgg tgatcgccgc agcgagggtc gtgttcgcca
     3661 cgaccttcgt gaaggtgccc tgcttgtcct tgggaacggg gatcgagatc aggttggagt
     3721 tgccccacac gtagatctct ccgccttcgg tgagggcgat cgagatctgg tggcctgcga
     3781 tgacctgctt cacttttcgg ctcgagagct cgggaggaat catgttcagc gtgaaccgat
     3841 catttcccca ggtatacacc ttgccgtcgg catcgagggc gacggcgtga tccaggccgg
     3901 cggaagcctg cacataacgc ttgtcggcgg ggggcatctt ctgcagtttc gccgacaagg
     3961 ttccccattg ataaagcttt ccatcccggt cgaccccgac gccgaagacc gatcctccgt
     4021 cgatcgaggc gacattgccc tgaagagcct tgtcatactt catataggag aaggtgggtc
     4081 tggtattctg ttgggtcgga tcctgaaatt gggtatccaa cgggaagaag atcggcagaa
     4141 tgaagaccgc caggaaaatt gccaggaaca tatagacacc gagcatggcg ctccactttt
     4201 cccggaagtt tcggagcatc gtccgatagg ggctctgcac ctgctcctcc tccaaaatgg
     4261 agagggtcgc ccgctggcgg ccgagaaaaa aggttttaag gacgtcggta aacgttgatc
     4321 ccaagggttt ttttgccatc gtatagtact cctaattcag tttgacccg
//
LOCUS       DAOPUZ010000034         4360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_20274_length_4360_cov_3.560511, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000034 DAOPUZ010000000
VERSION     DAOPUZ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20274_length_4360_cov_3.560511"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PKN79_01170"
     CDS             complement(<1..250)
                     /locus_tag="PKN79_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase"
                     /protein_id="HNZ94404.1"
                     /translation="MNHNDPVAISAENIASLRFTTYSFETDSLGAAQTAFYLRIVQEA
                     AGLHAAMRGCSIMDLHAEGKTWVITRNRIEIFGYTRWNE"
     gene            complement(247..789)
                     /gene="rsmD"
                     /locus_tag="PKN79_01175"
     CDS             complement(247..789)
                     /gene="rsmD"
                     /locus_tag="PKN79_01175"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNZ94405.1"
                     /translation="MRVTGGLYRGRKIVCPPGIIRPAMDRMRESMFSILGNQEGTAWL
                     DLFSGSGSIGIEAASRGAGLVHLVERDRGKKETIMKNIAFVEIPIKLFISDVFRFLMQ
                     AKIAYDIVYADPPFPMEEKIRLLEAVERNKILKAEGLFIIHYPVEEKAQWPEQLGDLL
                     FIDERSYGRSTLRFYKRKKR"
     gene            complement(832..2907)
                     /gene="recG"
                     /locus_tag="PKN79_01180"
     CDS             complement(832..2907)
                     /gene="recG"
                     /locus_tag="PKN79_01180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNZ94406.1"
                     /translation="MTYLRTITDPVAKLGGVGPAATRSYAELGIHTQSELLLLAPRTW
                     EDRSTVRPLGSVQDGQVANTLVEVLSHSYFGLKKGMKRTLKIIVRDVSGLGDGRLSLL
                     CFGRNFLEKTIKPGRIYYLWAPVAIKGSEKQAVQFELHPATEDGDFPPQFGKILPVYP
                     LRGSLTQRLIRANIRQVLTRCGAFINELPESLMKELDLLSTDEAIRAYHFPPNLQQLA
                     RARRTLAFTELFYLQLVARRKPRKKPAKPAIPGDLELRLIERLPFTLTNDQLAALKEI
                     REDLGAEEPMNRLLQGDVGSGKTLVGWISALKIIAEGGQVAFMAPTELLARQHAESGA
                     ALLEPLGVRLAFLTGSVKPKERSYLLTALQRGEIDLLVGTHALFSESVTFRNLRYVII
                     DEQHRFGVEQRLALLGKATEPHLLLMTATPIPRTLSLTVFGSLNISTLRTMPPGRKPV
                     ITLLVKEESRPRMYKSVGVEFTRGHQAYFIYPRIDDSGQSDLRDVTGMYEFLKQQYPG
                     VPSALIHSKLDEEEKVAILEAYRKGELSYLVSTSVVEVGIDIPNATCMIIEHADRFGL
                     AALHQLRGRVGRSALQSYCFLVFSDELTEEAKKRLQVMKDSTDGFHIAEQDLIIRGPG
                     ELTGRAQSGYLNLAFASLADDLELIEQARAASDRILQVDSGFLTPENTIIRSVLSEAP
                     PFEAGRSEA"
     gene            complement(2910..3521)
                     /locus_tag="PKN79_01185"
     CDS             complement(2910..3521)
                     /locus_tag="PKN79_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNZ94407.1"
                     /translation="MQQTPTVLMTIANLLASLLSLYSLLIWIRIILSWLKTGTENPLA
                     YYLGKIVDPYLSWFKGIRSLVRPRFDLTPLVALAVLSVIQSLLRLFGSYGTITVTMVI
                     ALLLQTLYSYIVHPILFFFFILLVVRLYFCYKRSIHSITYIALLDSLVGGLLDWTHRT
                     FYARQTVNDRKLVISSLVVLIIAWIGSSLLLRLLLSLLTKTGI"
     gene            complement(3555..>4360)
                     /locus_tag="PKN79_01190"
     CDS             complement(3555..>4360)
                     /locus_tag="PKN79_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNZ94408.1"
                     /translation="TNRPTLLENLPDAVASVQRKKEFSAYGQISLFDEESEEMMDSFV
                     MKEVEDWSTQEKLEIEKSLLGFYVSGHPLDRYRSAIKTRVTVDTARMEHIPLGQQTNI
                     IALLTQLKPHVTKRNDTIAFVQLTDLNTSFEGVIFADEYERVRHFLQIDRIYGFEGTF
                     DKRGNGDERISFRIDSVKTDPNELAPRAVSRCHVLLEKSFCTAEQITRLRDAALDFGG
                     SCTLYLYFKEQGRTLPSLVECGREFSVRCTADLAIELKKNSAVLDVWFD"
BASE COUNT          915 a         1192 c         1318 g          935 t
ORIGIN      
        1 gttcattcca ccgggtgtag ccgaagatct cgatccggtt ccgggtgatc acccacgtct
       61 tgccctcggc gtgcagatcc atgatcgaac aacctctcat cgccgcgtgc aagccggccg
      121 cttcctggac gatgcggagg tagaacgccg tctgggcggc ccccaggctg tcggtttcaa
      181 agctgtacgt ggtaaaccgc aagcttgcga tgttttcggc gctgattgcg accggatcat
      241 tgtggttcat ctttttttcc ttttatagaa ccgcagggtg ctgcggccgt atgagcgttc
      301 atcgatgaag agaagatcgc ccagctgctc cggccactgt gctttctctt cgaccgggta
      361 gtggatgata aacaatcctt ccgctttgag gatcttgttt cgttcaactg cttcaaggag
      421 gcggatcttc tcttccatcg gaaagggggg atcggcatag acgatatcat atgcgatctt
      481 cgcctgcata agaaatcgga agacatcgct tatgaagagc ttgatcggga tctcgacgaa
      541 ggcgatgttc ttcatgatcg tctctttctt gccccggtcc cgctccacca gatggacaag
      601 accggctccg cggctggcgg cttcaatgcc gatggatccg cttccgctga agagatcgag
      661 ccaggccgtt ccttcctgat ttccgaggat tgaaaacatt gactcgcgca tccgatccat
      721 agccggacga atgatgccgg gcgggcagac gatcttcctt cctcggtaga ggcctcctgt
      781 tacacgcata tgttcatcct agtggttttc taacgtttgc tccagaggac tctacgcttc
      841 gctccgcccg gcttcaaacg gtggggcttc actgaggact gagcggatga tcgtattttc
      901 gggggtaagg aagccgctgt cgacttgaag gatccggtcc gaggccgccc tggcctgttc
      961 gatcagctcc agatcatccg ccagcgaggc gaaggccaga ttcaggtagc cgctttgagc
     1021 ccgaccggtc aattcgccgg gaccgcggat gatgagatcc tgttcggcga tatggaaccc
     1081 gtcggtggaa tccttcatca cctggaggcg cttcttcgcc tcttcggtca gttcgtcgga
     1141 gaagacgagg aaacaatagg attgcagggc ggagcgcccg acccgcccgc gcagctggtg
     1201 cagggcggca aggccgaagc gatcggcatg ctcgatgatc atgcaggtgg cgttgggaat
     1261 gtcgatgccg acctccacga cgctggtgga gacaagatag gagagctcgc ccttccggta
     1321 cgcttcgaga atcgctacct tctcctcttc atccagcttg gagtggatca acgccgaggg
     1381 aacgccggga tactgctgct tgaggaactc gtacatgccc gttacgtccc gaaggtcgct
     1441 ctgtccgctg tcatcgatcc ggggatagat gaagtacgcc tgatgacccc gggtgaactc
     1501 gactccgacc gacttgtaca tccgaggcct actctcctct ttcaccagaa gggtgatgac
     1561 cggcttgcgc cccggcggca tcgtccgaag ggtcgaaatg ttcagcgacc cgaagacggt
     1621 gagggaaagg gtccggggaa tcggggtggc ggtcatcaga aggaggtgcg gctccgtcgc
     1681 cttgccgagg agagcgaggc gctgctccac cccgaagcgg tgctgctcgt cgatgatcac
     1741 ataccggagg ttcctgaatg tcacgctctc gctgaagagg gcatgggttc ccaccaacag
     1801 gtcgatctcc ccccgctgca aggcggtcag cagataagag cgctctttcg gcttcaccga
     1861 gccggtgaga aacgccagac ggactcccaa gggttccaac agcgccgccc cgctctcggc
     1921 gtgctgccgg gccaagagct cggtgggggc catgaacgcc acctgacccc cttcggcgat
     1981 gatcttcagg gcgctgatcc atccgaccag agtttttccc gagcccacat ccccctgaag
     2041 gaggcggttc atcggctcct ccgcaccgag gtcttcgcgg atctctttca gcgcggccag
     2101 ctgatcattc gtcaaggtga agggaaggcg ctcgatgagg cgaagctcca agtcgccggg
     2161 aatcgccggc ttggccggtt tttttctcgg ctttcgtcgg gctacgagct gaagatagaa
     2221 caattcggtg aaggcgagcg ttcttcgggc ccgggcaagc tgctgaaggt tcgggggaaa
     2281 gtggtaggcg cggatcgcct cgtcggtcga tagaagatcc agctccttca tcagcgattc
     2341 gggcagttca ttgatgaacg ctccgcaccg ggtgagaacc tgccggatgt tcgcccggat
     2401 gaggcgctgg gtaagggaac cgcgcagcgg atagacggga agaatcttcc cgaactgcgg
     2461 gggaaaatcc ccgtcctcgg tggcggggtg aagttcgaat tgaacggcct gcttctccga
     2521 acccttgatc gcaaccgggg cccaaagata ataaatcctt cccggtttga tcgtcttttc
     2581 cagaaagttc cggccgaagc agaggagcga aaggcgcccg tcgccaaggc cgctgacatc
     2641 gcggacgatg atcttgaggg tccgtttcat cccttttttc aacccgaaat aggagtggct
     2701 gagcacctcc acgagggtgt tggcgacctg gccgtcctgc acgctgccca atggacgcac
     2761 cgttgaacgg tcttcccacg ttcgcggggc gaggagcagc agctcgctct gggtgtggat
     2821 gcccagctcc gcatatgatc tggtcgccgc cggacccacg ccgccgagct tggcaaccgg
     2881 atcggtgatc gttcggagat aggtcatcgt cagattcctg ttttagtgag caacgaaagc
     2941 aggaggcgga gcagaaggga ggaaccgatc catgcgatga tcaggacgac caggcttgag
     3001 atcaccaact tccggtcgtt aaccgtttga cgggcataga aggttcgatg ggtccaatcc
     3061 aaaagccccc cgacaaggga gtcgagcagc gctatatagg tgattgagtg gatcgagcgt
     3121 ttataacaga agtacagtcg aacgaccaac aggatgaaaa agaagaagag aatcggatgg
     3181 acgatgtagg agtagagggt ctgaagcagc agggcgatca ccatcgtgac ggtgatggtc
     3241 ccgtagcttc caaagagcct taagagggat tggatcactg aaaggactgc aagggctaca
     3301 agcggagtca gatcgaaccg ggggcggaca agactgcgaa tccccttgaa ccatgagagg
     3361 tacggatcca cgatctttcc gagatagtag gctagaggat tctccgtgcc ggttttcagc
     3421 catgaaagga tgattcgaat ccagatgaga agcgaataca gcgacaggag gctggctaaa
     3481 agatttgcta ttgtcatcaa gaccgtgggg gtttgctgca tatcgatcga acactccttg
     3541 gctctatcat actcttagtc gaaccaaacg tccaagactg cggaattctt tttcagctca
     3601 atggccaaat cggcagtgca ccggactgaa aactcccgtc cgcattcaac aagggagggg
     3661 agcgttctgc cctgttcctt gaagtaaaga tacagggtgc aggaaccgcc gaagtcgagc
     3721 gcggcatccc tcagacgagt gatctgctcg gcggtgcaga aggatttctc caacaggacg
     3781 tggcagcggc tgaccgcccg gggagccagc tcgttgggat cggttttgac gctgtcgatt
     3841 cggaaggaga ttcgttcatc tccattgccc cgcttgtcaa aggttccttc aaagccgtag
     3901 attcggtcga tttggaggaa gtggcggacc cgttcatact catcggcgaa gatcacccct
     3961 tcgaaggagg tgttcagatc ggtaagctgg acgaacgcga tcgtgtcgtt ccgctttgta
     4021 acatgcggtt tgagctgggt gagcagcgcg atgatgttgg tctgttggcc caaggggatg
     4081 tgctccatcc gggcggtgtc gaccgtcacc cgggttttga tcgcgctgcg atagcgatcg
     4141 agagggtgtc cgcttacata gaagccgagg agggattttt caatttccag cttttcctga
     4201 gtcgaccaat cctccacctc cttcattacg aacgagtcca tcatctcctc gctctcttca
     4261 tcgaagaggg agatttgccc atatgctgaa aactctttct ttcgttggac ggaggcgacc
     4321 gcatcgggaa ggttctccag caatgtggga cggttggtcc
//
LOCUS       DAOPUZ010000035         4330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_20435_length_4330_cov_2.989942, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000035 DAOPUZ010000000
VERSION     DAOPUZ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20435_length_4330_cov_2.989942"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..647
                     /locus_tag="PKN79_01195"
     CDS             36..647
                     /locus_tag="PKN79_01195"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ94409.1"
                     /translation="MLFHWISRIYGWFFHYQKRRFKAILTEHGSELESVESACDVGCG
                     TGALCAVLAERGIRTTGVDSAKGMLAVARRKTRGLPIRFILADGVSGLPFLDKSFDLV
                     ISSHVAHGLKKPDRIRFYQELKRIASKTVIIHDYNSRRHPVSTIIEWAEGGDYFNFIK
                     EPKKEIEEIFPSISIVDVSPYFRWYVCRCATEPSSGDTPASSR"
     gene            complement(604..1941)
                     /locus_tag="PKN79_01200"
     CDS             complement(604..1941)
                     /locus_tag="PKN79_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ94410.1"
                     /translation="MKRFIILAAITLLAIPMLFAQAQEESTPYTTITWWHPNSGLVGT
                     ASEELIKTFNRTVGEDLKIRVEGVYQGKANDVLTKLKPILQSKDQRDLPDLAQLDGSA
                     VLDIRDSAQLITINELARNEPEYLSKIVEATLNSITYQGKVLAMPFNASTILLYYNKD
                     AFAKAGISKPPRTLDDVKTAAAKLRQTDDKGRTVFWGIANVPTTYELMVWLGHQNGLT
                     YLTDFENGHIGAPTRVLFDENGTMVNFLTKWQAIYDTGSLANLTSDLNGAFASGQVAM
                     ILASTSNLSTIVSMVGDRFEVGVANMPMVDENATGGVNVGGGAIWALDNGKNNQWAIW
                     EFLKFATSAEEQLKWHIATGYFPVNVDTYELPAFKSHLQEHPLFGVASRQLMDSNPKL
                     QGIWVPSAYQIYYAFQSGILKMLEEKQTPQAATKAIAQEINGYLQEYHLMKAR"
     gene            complement(1946..2752)
                     /locus_tag="PKN79_01205"
     CDS             complement(1946..2752)
                     /locus_tag="PKN79_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ94411.1"
                     /translation="MKTLRATLSTLLSLLIVFPIVYSISASFFQYTDFTALPARLFPS
                     RLNIGNYVRAFSESFLARFLLNSLLTAIVGTLLRMAISILAAYTFSFFTFKGRNALFI
                     LIVATMLLPSDALIIANFMTIRALRLTDTYLGIIATSLLAPTHIFMLRQYFRMMSVEY
                     REAAIIEGCSDSRFLSMLLMPMSKAVILTLAIHSFSTIFNDYLWPLLVTNKTSMRTVQ
                     VGLTMMGFSEQLDYGPQFAAITFLMTPILIVFIALHKPIIESVSIRFTGR"
     gene            complement(2749..3576)
                     /locus_tag="PKN79_01210"
     CDS             complement(2749..3576)
                     /locus_tag="PKN79_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ94412.1"
                     /translation="MIFPALLLAVLFSYVPFILTFLNSVHAVSLGGKRLGFVGLANYR
                     RLFGNPSFHASLSNTLRFTLFFVPTNIIVTLLAAVLSNRKGWLANLNQIFFFLPMAVG
                     MSSMLMILKMMFNPTIGIINRLIGVNIQWFNDPNAAMALLVIAGVFLDIGFDFLLFSA
                     ALRNVPNDLKEVALINGANGFQIFRHLELPLIAPTLIFIVVTNIKNAMLISAPVLILT
                     EGGPFRSTQTLVYQMYLEGFKSGNYALGSAIAIVVFLLTFAALLILLHLERRRIYYR"
     gene            complement(3774..>4330)
                     /locus_tag="PKN79_01215"
     CDS             complement(3774..>4330)
                     /locus_tag="PKN79_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HNZ94413.1"
                     /translation="VKMFMKLVGIETTIDNATCNRINGLSVDLTVAASLGAISLVAVK
                     GYWVPIVALSITGIIITAFILPWYTSRLYDDHQFLRMLLLYGTATGTLPTGLALLRVV
                     DQEFETPVATDYLYSVGIVFILAIPFILTINFPAFAVTKNNPVFFYLTILIAGVYLFG
                     SFVAYLLIAKKRSFIQPKKLFYVE"
BASE COUNT         1099 a         1044 c         1222 g          965 t
ORIGIN      
        1 cttcaaagag aagtgccgct tcaaggacag ctaagatgct ctttcattgg atcagcagaa
       61 tctacggttg gttcttccac tatcagaaac gccggttcaa agcgattctt accgagcacg
      121 gcagtgaact tgaatcggtt gagagtgcgt gcgatgtcgg ttgcggcacc ggagcgctgt
      181 gtgccgttct ggctgaacgg ggaatccgaa caaccggagt ggattcagcc aaaggaatgc
      241 ttgccgtagc ccgaagaaaa acccgcggtc ttcccatccg gtttatcttg gctgacgggg
      301 tatcggggtt gccgttcctg gacaagagtt tcgatctcgt catttccagc cacgtcgccc
      361 atggcctgaa gaagcccgat cgaattcggt tctatcagga actgaagcga atcgcatcaa
      421 agaccgtgat catccacgac tacaacagcc gtcggcaccc tgtcagcacg atcatcgaat
      481 gggcggaggg cggcgattac ttcaacttta tcaaagaacc gaaaaaagag atcgaagaga
      541 tcttcccctc catctcgatc gtggacgtaa gtccctattt caggtggtat gtctgccgct
      601 gcgctaccga gccttcatca ggtgatactc ctgcaagtag ccgttgatct cctgagcgat
      661 cgccttggtt gccgcctgag gcgtctgctt ctcctccagc atcttcaaga ttcccgattg
      721 gaacgcatag tagatctgat aggcgctggg aacccaaatg ccttggagct tcgggtttga
      781 atccatcagc tggcggcttg cgactccgaa gagcggatgc tcttgaaggt ggcttttaaa
      841 tgccggcagt tcgtaggtat ccacgttcac cgggaagtag ccggtggcga tatgccactt
      901 gagctgctct tcggcgctgg tggcaaattt gaggaactcc caaatcgccc attgattgtt
      961 tttaccgttg tcgagcgccc agatcgctcc gccgccgaca ttgacccctc cggttgcgtt
     1021 ttcatcgacc atcggcatat tggcgacgcc gacttcaaag cggtcgccga ccatcgaaac
     1081 gattgtagag aggttgctcg ttgaagcgag aatcatcgcc acctggccgg aagcgaacgc
     1141 tccgttgaga tcgcttgtga ggtttgccag cgatccggta tcatagatcg cctgccattt
     1201 ggtcaggaag ttcaccatcg ttccgttctc atcgaagagg acccgggtcg gggctccgat
     1261 atgtccgttc tcaaaatcgg tgaggtaggt gagtccgttc tggtggccaa gccagaccat
     1321 cagctcatac gtggtgggga cgttcgcgat accccagaac acggttcgcc ctttatcgtc
     1381 ggtttgcctc agtttcgcag cggctgtttt cacatcatcc aaggttctgg gcggtttgga
     1441 gattcccgct ttggcaaatg catctttatt gtagtagagc aggatcgtcg atgcgttgaa
     1501 gggcatagcg aggaccttgc cctgataggt tatcgagttg agcgtcgctt caacgatttt
     1561 ggagagatac tccggctcat tcctagccag ctcgtttatc gtgatgagct gggcggaatc
     1621 gcggatatcc agcaccgccg agccgtccag ctgggcaagg tcgggaagat cccgctgatc
     1681 tttggattga agaatcggtt taagtttggt aaggacatcg ttcgccttcc cctgatagac
     1741 cccttcaacg cggatcttca gatcttcgcc aaccgtccgg ttgaaggttt ttatcagctc
     1801 ctcgctggcc gtacccacca aacctgagtt ggggtgccac cacgtgatcg tggtgtacgg
     1861 tgtgctctct tcctgtgcct gagcgaaaag catcggtatc gcaagaagag tgatagccgc
     1921 caggatgatg aaacgtttca tgatattacc tcccggtaaa gcgtatcgat acgctttcta
     1981 taatcggttt atgaagagcg ataaagacga tgagaatcgg ggtcatcagg aaggtgatcg
     2041 ccgcaaattg ggggccgtag tcgagctgct cgctgaagcc catcatcgtc aaacccacct
     2101 ggaccgtcct catcgatgtt ttattggtca cgagaagcgg ccacaaataa tcgttgaaaa
     2161 tcgtggagaa ggagtgaatc gcaagggtga ggatcaccgc tttgctcatc ggcatcaaga
     2221 gcatcgacag aaaacgggaa tcggagcatc cctcgatgat agccgcttca cggtattcca
     2281 cgctcatcat tcgaaagtac tggcgcaaca tgaaaatatg agtcggagca agtaaacttg
     2341 tcgcgataat ccccagatag gtatcggtga gtcggagcgc ccggatcgtc atgaagttcg
     2401 cgatgatcaa ggcatcggag ggaaggagca tcgttgcaac gatgagaatg aagagagcat
     2461 tccgaccttt gaaggtgaaa aaggagaagg tgtaggcggc aagaattgaa atagccatgc
     2521 gcagcagcgt tccgacaatt gcggtgagca ggctgttcag cagaaagcga gccaggaaag
     2581 actcggaaaa ggcgcggaca tagttgccga tgttcaatcg ggagggaaaa agacgggccg
     2641 gcagggcggt gaaatcggtg tactggaaaa acgacgcact gatcgaatac acgatcggga
     2701 atacgataag aagggaaaga agggttgaaa gggttgcacg aagggtcttc accggtagta
     2761 gattctcctt cgttccaaat ggagcaggat gagaagggct gcgaacgtaa gaaggaagac
     2821 cacgatggcg atcgccgatc ccaaggcata gtttccgctc ttgaaccctt ccaagtacat
     2881 ctgatagacc agcgtctggg tggagcggaa cggcccgcct tcggtgagaa taagcaccgg
     2941 ggcgctgatg agcatcgcat ttttaatatt cgttacgacg atgaagatca aggttggcgc
     3001 gatgagcggc aattccagat gacgaaagat ctgaaatccg tttgccccgt tgatcagggc
     3061 tacctctttc aagtcattgg gaacgtttcg cagcgcagca ctgaagagca gaaagtcaaa
     3121 accgatatca agaaagacac cggcgatcac caggagagcc atcgccgcat tgggatcgtt
     3181 gaaccattga atgttgacac cgatgaggcg gttgatgatc ccgatcgtcg gattgaacat
     3241 catcttcagg atcatcagca tgctgctcat tccgaccgcc atcggcagga aaaagaagat
     3301 ctggttgaga tttgccagcc accctttccg gttggaaagg actgccgcca aaagggtgac
     3361 gatgatgttt gtcgggacga agaagagggt gaaccgaagt gtgtttgaaa ggctggcatg
     3421 gaacgagggg tttccaaaga gccgtcgata gttggcgagg ccaacgaacc caagtcgttt
     3481 accaccgagc gaaacggcgt gaacactgtt caggaaggta agaataaagg gaacatagct
     3541 aaagagcacc gccagcaaaa gtgcgggaaa gatcatgata tatggcttgc atgttatcga
     3601 ttctttagat ctcattcgca tcatcccaat cgatactagc ccctttgacg atgattgaac
     3661 aacccaaaac aatggacaaa aagagaaatt taggtaatat atgtgacctt tttgagagta
     3721 acgtcacctt tggggataaa agaggcgcct cttatcaaag aagcgccccg acatcactcg
     3781 acatagaaga gtttttttgg ttggatgaag gatcgttttt ttgcaatcag aaggtaggcg
     3841 acaaacgacc cgaagaggta caccccggcg atgagaatcg tgagatagaa gaagacgggg
     3901 ttgttcttcg tgacggcaaa tgccggaaaa ttgatcgtca aaatgaaggg aatggcaagg
     3961 ataaagacga ttcccaccga gtagagatag tcggtagcca ccggagtctc gaactcctgg
     4021 tctaccactc gaaggagcgc cagtccggta ggaagggttc ccgtcgcggt accgtacagc
     4081 aagagcattc ggaggaactg atggtcgtca tagaggcggg aggtgtacca gggaagaatg
     4141 aatgccgtga tgatgattcc cgttatcgag agcgcaacga tcggaaccca gtagcccttt
     4201 accgcgacaa ggctgatcgc tcccaggctt gcagcaacgg tcaggtccac ggagagaccg
     4261 ttgatccggt tgcacgtcgc attatcgatt gtcgtttcga tccccaccag tttcatgaac
     4321 atcttcactg
//
LOCUS       DAOPUZ010000036         4244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_21016_length_4244_cov_3.770351, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000036 DAOPUZ010000000
VERSION     DAOPUZ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21016_length_4244_cov_3.770351"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKN79_01220"
     CDS             <1..327
                     /locus_tag="PKN79_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNZ94414.1"
                     /translation="TSNGPIEVSSTSGRIEVESLRAPKITVNSVSGSVDVALPLPSPS
                     DVTLSSTSGQVEADLLGADHYTINAHTTSGSITIGGEQRSTLTKGDGSSRITLQTVSG
                     SISIRP"
     gene            602..2395
                     /gene="aspS"
                     /locus_tag="PKN79_01225"
     CDS             602..2395
                     /gene="aspS"
                     /locus_tag="PKN79_01225"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNZ94415.1"
                     /translation="MAEHFSKRTATCGGLTKADDKKAVVLNGWVHRHRDHGMIHFINL
                     RDRYGITQVIVDDDSSKELQQAALSLNLEDCIAVTGVVRARPASMVNPEMATGEIEVK
                     AEKIEILSTCAPLPFQIDDGNVPREELRLKYRYLDLRTEGMKKRIKLRHDMVWAIRRY
                     LSSRGFYEIETPTMIKSTPEGARDFLVPSRIHVGKFYALPQSPQLFKQILMVGGMDKY
                     FQIARCYRDEDARGDRQLEFTQLDLEMSFVTRDDVLSTMEGLFGYVFKEVMDYDLPPT
                     FRRFSYEEAMNTYGCDKPDLRFGLELQDMNDLASRSTFSTFKEIVAQGGHVKALCAPH
                     SEIVDFSRKYITDLENAAKVYGAHGLAWMKVGADKTASGGVSKFFEGLQEEVIDRFDA
                     KEGDMILFVAHNEWKKTCASLSAVRSKLGKDLQLIRPGFEFCWIVDFPLFEYNEERGQ
                     WEAAHHMFSMPQAEYIDTMEEDPGRVKGDLYDLVLNGYEVASGSIRIHDVELQKRIFR
                     ICNFDDETAQERFGFLLTAFSFSPPPHGGIAPGIDRIAMIMAGESSIHEVIAFPKNTA
                     GISPMDDSPSFVEEKQLEELHLKIVYPEKKE"
     gene            2430..3311
                     /locus_tag="PKN79_01230"
                     /pseudo
     CDS             2430..3311
                     /locus_tag="PKN79_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
     gene            complement(3331..>4244)
                     /locus_tag="PKN79_01235"
     CDS             complement(3331..>4244)
                     /locus_tag="PKN79_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94416.1"
                     /translation="PPCGSRLSHQESQGGEQRFMIRKERVYILVKTYPAISKKYNELV
                     CTAGILENGEWIRLYPIPFRILKEDQKFPKFSWIEVEIERNTEDFRPETYRPNLNSIC
                     VDPRPDKVDWKKRNDVIFKRTSVYRDLEKLLQDKSEKSISLAIFKPKRILDLVVKKTS
                     REWDLQKLETLREKAAQLDLFMTPEEVQAEFSVVPKVPYEFSYKFEDEKGKISTLMIS
                     DWEIGMLYFNCLKWTNNDEPEAVKQVKTKYLDFFSTRDVYLFLGTTKGYHNISDNPFI
                     IIGVYYPPKAVEQDSPQLSLFDSENGF"
BASE COUNT         1005 a         1165 c         1058 g         1016 t
ORIGIN      
        1 acgagcaatg gtccgattga agtctcatcg accagcggaa gaatcgaggt ggaatccctt
       61 cgggcgccca aaatcaccgt caattcggta agcggaagcg tggacgtggc cctcccgctg
      121 ccttcgccct ccgatgtcac cctctcctcc accagcggac aggtggaggc ggatctgctt
      181 ggagccgatc attacaccat caatgcgcac accaccagcg gctcgatcac gatcggaggc
      241 gagcagcgct ccaccctgac aaaaggagac ggatcttcca gaatcaccct tcaaacggtc
      301 agcggttcga tttccattcg tccatagaaa accggtcatt cgatttcttc cccgcacccg
      361 cggggatgtt ttttgtgaag ccacgaaaaa cgcaccccct cggagtgcgc tcttcatcaa
      421 accgggtact tgaaaggttc gcggacctaa ccacttcgcc ggtacagatc cagtattgat
      481 cccttgcata cgctttgtca atattgccgg tcccaagcac cccggtacgt atgagagtgt
      541 ttcaatcaat tgccatactt ccccctatcg tatagtattt gtttacttga agggggttca
      601 catggctgaa catttttcaa agcgtaccgc gacatgcggc ggtttaacca aagccgacga
      661 caagaaggcc gtcgttctga acggctgggt acaccggcat cgcgaccatg gaatgattca
      721 tttcatcaac cttcgcgacc gctacggaat cacccaggtc atcgtcgatg acgattcatc
      781 aaaagagctg cagcaggctg ccctttcatt gaaccttgaa gactgcattg ccgttaccgg
      841 tgtggttcgg gcccgccccg cttcgatggt gaatcccgag atggcgaccg gcgaaatcga
      901 agtgaaagcg gaaaaaatcg agattctttc aacctgcgca ccgcttccct tccagatcga
      961 tgacggcaac gttccccggg aggagctgcg gctgaagtat cgatacctcg atcttcggac
     1021 cgaggggatg aagaagcgga tcaagctgcg ccatgatatg gtctgggcga tccgccgcta
     1081 tctttcatcc cgcggtttct acgagatcga gactccgacg atgatcaagt ccacccccga
     1141 gggggctcgt gacttcctcg ttccttcccg aatccatgtg ggcaagttct atgctctgcc
     1201 ccagagcccc cagctcttca agcagattct gatggtcgga gggatggaca agtacttcca
     1261 gatcgcccgc tgctaccgcg acgaggatgc ccggggcgac cgccagttgg agttcaccca
     1321 gctggatctg gagatgagct tcgtgacgcg ggatgatgtc ctctccacga tggaagggct
     1381 gttcggatat gtcttcaagg aagtgatgga ctacgatctt ccccccacct tccggcggtt
     1441 ctcctatgag gaagcgatga acacctacgg gtgcgacaag ccggatctgc gcttcggtct
     1501 ggagctgcag gatatgaacg accttgcttc ccgctccacg ttctccacgt tcaaggagat
     1561 cgtcgcccag ggcggccacg tcaaggcgct gtgcgcacct cattcggaaa tcgtcgattt
     1621 ttcccgcaaa tacatcaccg atctggagaa cgctgcgaaa gtctatgggg ctcatggcct
     1681 tgcgtggatg aaagtcggag cggacaagac cgcaagcggc ggcgtttcca agttcttcga
     1741 gggtctccaa gaggaggtca tcgaccgctt cgatgcgaaa gagggcgaca tgattctctt
     1801 tgtcgcccac aatgagtgga aaaagacctg tgcaagcctc agtgcagtcc gctccaagct
     1861 cggaaaagac ctccagctga tcaggccggg cttcgaattc tgctggattg tcgacttccc
     1921 tctctttgag tacaacgagg agcgcggtca atgggaagcc gcccaccaca tgttcagcat
     1981 gccgcaggcg gagtacatcg atacgatgga agaggatccg ggtcgggtga agggagacct
     2041 gtacgacctc gtgctcaacg ggtatgaagt ggcttcggga tcgatccgga ttcacgatgt
     2101 cgagctgcag aagcgaatct tccgcatctg caattttgac gacgagaccg ctcaggagcg
     2161 cttcggcttc ctcctcactg cgttcagctt cagccctccg ccccacggag ggatcgcccc
     2221 gggaatcgac cggatcgcca tgatcatggc cggtgaaagc tcgatccacg aagtgatcgc
     2281 attcccgaaa aacactgcgg gtatcagtcc gatggatgat tctccttcct tcgtggaaga
     2341 gaagcagctt gaagagcttc acctgaagat tgtctaccct gagaagaagg agtaaccgaa
     2401 aagccccccg atccgggggc tttccacata tgagcaaatc cacattggca acgaccgttg
     2461 cccggatcaa tctaatcagc aatgcttttt tagccatcgc taaaatcctc atcggatact
     2521 tcgcccactc cgccgccttg ctgaacgacg gtatcaacaa tctgggggac accctttcct
     2581 cgatcatcac gatggtcggc atctccgccg gtgcgaagaa ggcggatgat gatcatcaat
     2641 acgggcacga gcgccttgag tgcgtggcgg cgattctgct gtccggcatc atcatgttcg
     2701 tcggaatcgc cctcttcggc agcggtgtct catcgatcat taaaagaacc tatctggaca
     2761 cccccgttcc cgcgttcccg gccgtgatcg cttcgcttgc ttcgatcatc atcaaaggag
     2821 cgatgtatat atatactcga tgggccggct aaaaggtcgg ctcccccgct ctgatggcct
     2881 cggcgggcga ctcgttgagc gacgtgtttg ccacctccgg agggctcatc ggcatcctct
     2941 tttcccggat ggggtatccg atcggggatg cggtcgcagc ggtggtcatc gccctcttca
     3001 tcttccgggt cggcgtccag atcttccggg acggaatgaa ccagatggtg gatcgagcct
     3061 gcgacgagtc cttcaccaaa gaggtcagag ccctcatcga agcacaggaa ggagtcttga
     3121 aagtcgattt attacgaaca cgacaattcg gttcgagatg ctacgttgac gtggagatcg
     3181 ccgccgaccc gaaccagacg ctcaccgaag cccactcgat cgccgaacgc gtccacaaga
     3241 cgatcgaagc ctccttcccc acggtcaagc actgcatggt gcatgtgaac ccgagcagga
     3301 agaaacgata aaacgtggga gctcttattt ttaaaaacca ttctctgaat caaatagcga
     3361 tagttgagga gaatcctgtt cgactgcttt aggaggataa tatactccaa taatgatgaa
     3421 tggattatct gatatattgt gatacccctt cgtggttcca agaaacaagt atacgtcccg
     3481 cgttgagaag aaatctaaat actttgtttt gacctgtttg acagcttcag gttcatcatt
     3541 attcgtccac tttaaacagt tgaaataaag cattccgatt tcccaatctg aaatcataag
     3601 tgtggagatc ttacctttct catcctcaaa tttgtaggaa aactcatatg ggactttcgg
     3661 tacgacacta aactctgcct gaacttcttc aggggtcata aacaaatcca actgagcggc
     3721 tttttctctt agcgtctcaa gcttttgtag atcccattcg cgagaagtct tttttaccac
     3781 caaatcaaga atcctctttg gcttaaaaat tgccaaagat atagatttct ctgacttatc
     3841 ttgcaagagc ttttccaaat ccctatagac agatgtcctt ttaaagatga catcattgcg
     3901 ctttttccaa tcaaccttat ctggtctggg atcaacacaa atactgttta ggttgggacg
     3961 gtatgtttca ggacgaaagt cttctgtatt gcgctcgatt tcaacctcaa tccaagagaa
     4021 ttttggaaat ttttgatctt ctttcaatat tcgaaaagga attgggtata atcgtatcca
     4081 ctctccgttc tctagaatgc ctgcagtaca tacaagctca ttgtattttt tagaaatagc
     4141 cggatatgtt tttaccagaa tatatactcg ctctttcctg atcataaatc tctgctctcc
     4201 accttgtgat tcctgatgag ataatctcga accacatggc ggtg
//
LOCUS       DAOPUZ010000037         4237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_21067_length_4237_cov_2.978001, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000037 DAOPUZ010000000
VERSION     DAOPUZ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21067_length_4237_cov_2.978001"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKN79_01240"
     CDS             <1..452
                     /locus_tag="PKN79_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNZ94417.1"
                     /translation="ILSVTGEGIVNPKNVLAPIGTKVSDLLEFCGGLQGEVEKLIIGG
                     PMMGFAFFDTETPITKGSDGILALAAKKKARQTPCISCGKCVDACPIGLVPTLLYKQI
                     TNHNYEVAMNSLSLMDCKECGCCSYVCPAHLPLVHTFKTGKKMGRKK"
     gene            452..1543
                     /locus_tag="PKN79_01245"
     CDS             452..1543
                     /locus_tag="PKN79_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNZ94418.1"
                     /translation="MTEQPLMTVSSSPHLHDGSSTANIMWHVSLSLVPAALWGIYVFG
                     LYSLVIILVSIVSAALTEFLLGKIGKECTIWDGSAVLTGFLVGLNMPPSIPLFIPIVA
                     SFFAIFVAKWTFGGLGANWANPALSGRVFVFFSFTTQMSTFTTPRTLLEAMPDTLSGA
                     TPLGFMKTAVSGGSCGATTNELLSWVGYPATPIAQKIGAVLGINAYTIDTFLGNVAGS
                     IGEVSALLLLIGGIYLLIRRIITWHIPVTYLLSSALLSRVFGGLPLGLGIFQGTFFPS
                     MFSGGLLFGALFMATDYVTSPVSSKGKIIFGIGCGFFTFLFRSFGSMPEAVSVSILLM
                     NIITPTIDRYIRPRKFGEKKLFARKEAVA"
     gene            1540..1755
                     /locus_tag="PKN79_01250"
     CDS             1540..1755
                     /locus_tag="PKN79_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94419.1"
                     /translation="MKKNLKYSLILLSICAISTALLALTNAITAPVIERQGQASRLAG
                     LEAVSMGYEAGAQKVVTDDPAVTFMIN"
     gene            1818..2093
                     /locus_tag="PKN79_01255"
     CDS             1818..2093
                     /locus_tag="PKN79_01255"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNZ94420.1"
                     /translation="MTLVASYNTAGEILRVQLLSHNETPGLGKKAENEGYMDKFEGTG
                     AAQAVPTNKSMLNSEDAAAVSGSTVTFVAIGKALEAGSRYVIGLGGK"
     gene            2093..2713
                     /gene="rsxE"
                     /locus_tag="PKN79_01260"
     CDS             2093..2713
                     /gene="rsxE"
                     /locus_tag="PKN79_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HNZ94421.1"
                     /translation="MNIKELTKGIFKENPVFIIVLGLCPVLGVSTQVSNAIGMSAGVL
                     FVLLFSNIIISALRNIIPDSIHIPAYIVIIASLVTIIEMLMHAFAPAVYAALGVYLPL
                     IVVNCIILGRAEAFASSNTVLDSALDAIGMSIGFALSLTLIALIREVLGAGTITLFPV
                     GSFSGVIEVPWLVDNPFKVMALAPGALLLMGFLKAFFDWKAMRKAG"
     gene            2715..3308
                     /gene="rsxA"
                     /locus_tag="PKN79_01265"
     CDS             2715..3308
                     /gene="rsxA"
                     /locus_tag="PKN79_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNZ94422.1"
                     /translation="MSYIAILITFIFINNFILVQFLGLCPFIGVSKNSENAIGMGASV
                     TFVTTVASVVTWAIYHFLLVPFNLAYLQTLTFILVIATLVQLLEMVIQKVSPPLYKAL
                     GIYLPLITTNCAVLGIAIINITNNYNVMESFTAGLAAGLGFTLAIVLMSNVRERLDLH
                     PVRKAYRGMPIAFISAGLMALAFMAFDKSLLTNLHLV"
     gene            3318..4118
                     /locus_tag="PKN79_01270"
     CDS             3318..4118
                     /locus_tag="PKN79_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNZ94423.1"
                     /translation="MNILLAIIIVAVLGGIFGYGLSYAEKKLAIEKDPKVLALEPIMP
                     GANCGICGYAGCNAYVAAVVKGEAELGLCTPGGPSLVEKMSEIMGLKVDPVGEVRRMV
                     AHVMCRGNPEVTKRDYRYEGLEDCNAAALLLGGDTTCKSACLRLGSCINVCPTGAISK
                     DEKGYIIVDEKKCISCEKCVLVCPTGAMQMIGEDDEYAIECNNHEPGGKVRKYCTVGC
                     IGCKICETKYPESGCTVDRFLATFNQLAEHNQIADAAEACPTKCIVKL"
BASE COUNT          877 a         1104 c         1236 g         1020 t
ORIGIN      
        1 gaatcctcag cgtaacgggg gaggggatcg tcaatccgaa gaatgttctt gctccgatcg
       61 gaacgaaagt gagtgatctg cttgagtttt gcggcggtct tcagggcgaa gtcgaaaagc
      121 ttatcatcgg cggaccgatg atgggcttcg cctttttcga taccgaaact ccgataacga
      181 aaggatccga cggaattctg gcgcttgcgg caaagaagaa ggcccgtcag actccctgca
      241 tcagttgcgg aaagtgcgtc gatgcctgtc cgatcggctt ggtgcccacg ctgctgtaca
      301 agcagatcac caaccacaac tacgaggttg cgatgaattc cttgagtttg atggattgca
      361 aggagtgcgg ctgctgctcc tatgtctgtc ccgctcactt gccgcttgtt cataccttca
      421 agaccggcaa gaagatgggg aggaagaaat gatgactgaa caacctttga tgaccgtatc
      481 ctcatcaccg cacctgcacg acgggagttc gaccgccaac atcatgtggc acgtgtcgct
      541 ttctctggtg ccggcggcgt tgtggggaat ctacgtcttc ggactgtaca gccttgtgat
      601 cattctcgtt tcgatcgtga gcgccgcgtt gacggagttc ctgctcggga agatcggcaa
      661 ggagtgcacc atctgggacg ggtcggcggt cctgaccggc tttctggtcg gcttgaacat
      721 gccgccctcg ataccgctct tcattccgat cgtcgcctcc ttcttcgcga tcttcgtcgc
      781 caagtggacc ttcggcggac ttggagcgaa ctgggcgaac cccgccctca gcggccgggt
      841 cttcgtcttc ttcagcttca ccacccagat gagtaccttc acgactcccc ggacgcttct
      901 ggaggcgatg cccgacaccc tcagcggggc gaccccgctg ggattcatga agacggcggt
      961 ctcaggcggt tcgtgcggag ccaccaccaa cgagctgctt tcctgggtgg gctatccggc
     1021 aacgccaatc gcacagaaga tcggcgcggt tctcggaatc aatgcgtaca cgatcgatac
     1081 cttcctggga aatgtcgcag gatcaatcgg tgaagtcagt gcgcttctct tgctgatcgg
     1141 ggggatctac ctgctgatcc gcaggatcat cacgtggcat atccccgtta cctatctgct
     1201 ttcgagcgct ctcctctcgc gggtcttcgg cgggcttccg ctcggtctgg ggatcttcca
     1261 ggggacgttc ttcccgtcga tgttcagcgg cggtcttctc ttcggcgcgc tgttcatggc
     1321 gaccgactac gtgaccagcc cggtctcctc caagggcaag atcatcttcg gcatcggctg
     1381 cggcttcttc acctttctct tcaggagctt cggttcgatg ccggaagcgg tcagtgtttc
     1441 aatccttttg atgaacatca tcaccccgac gatcgaccgc tatatccgac cgaggaagtt
     1501 cggagagaag aaactctttg cgcgaaagga ggcggtggca tgaagaagaa tctgaagtat
     1561 tcgctgatcc ttttatcgat ctgtgcgatc agcaccgctc tgctggccct taccaacgcg
     1621 atcaccgccc cggtcattga acggcaggga caggcgagcc ggctcgcggg tctcgaagcg
     1681 gtgagcatgg ggtatgaagc cggagcccag aaggttgtaa ccgatgatcc ggcggtcacc
     1741 ttcatgatca actgaaggat gatagacgga cggcgggata tatcctcggt ctcaaaggcg
     1801 ccgggtacgg cggtgagatg acgctggtgg cttcctacaa tacagccggg gagattcttc
     1861 gtgttcagct gctttcccac aacgaaacgc ccggtctggg gaagaaagcg gagaacgagg
     1921 gatatatgga caagttcgag gggaccggcg cggcacaggc ggttccgacg aacaagagca
     1981 tgctgaactc ggaagatgcc gccgcggtca gcggttccac ggtgacgttc gtcgcgatcg
     2041 gcaaggcgct cgaagcggga tcgcgctatg tgatcgggtt gggaggcaag tgatgaacat
     2101 aaaagaattg acaaagggaa tcttcaaaga aaacccggtt ttcatcatcg tgctgggcct
     2161 gtgccccgtc ctcggggtct ccactcaggt ctccaatgcg atcgggatga gcgcaggggt
     2221 gctctttgtg ctcctctttt cgaacatcat catcagtgcg ctcagaaaca tcatccccga
     2281 ttcgattcac attccggcgt acatcgtcat catcgcctcg ttggtgacga tcatcgagat
     2341 gttgatgcat gccttcgctc cggcggtcta tgcagcgttg ggtgtctatc ttcccctgat
     2401 tgtcgtgaac tgcatcatcc tcggtcgagc ggaagcgttc gcttcgagca ataccgtgct
     2461 cgattccgcc cttgatgcga tcgggatgtc gatcggattt gctttgagtc tgacgttgat
     2521 cgccctgatt cgcgaggttc ttggcgccgg aacgatcacc ttgttccccg tcggctcctt
     2581 cagcggagtg attgaggtgc cgtggctggt cgacaacccc ttcaaggtga tggccctcgc
     2641 tccgggagcc cttttattga tgggcttttt gaaagcgttc tttgactgga aggctatgag
     2701 gaaggcaggc tgatatgagt tatattgcaa tcttaatcac ctttattttc attaataact
     2761 ttattttggt ccaatttttg ggtctctgtc cctttatcgg agtttccaag aacagcgaga
     2821 acgcgatcgg gatgggagcg agcgttacct tcgtgaccac ggtcgcttcc gtcgtcacct
     2881 gggcaatcta ccatttcctg ttggttcctt tcaatcttgc ctatctccag acgttgacgt
     2941 tcatcctggt gatcgccacc ttggttcagc tgttggaaat ggttattcag aaggtgagcc
     3001 cccccctcta caaggcgctg gggatctacc ttccgctcat tacgacgaac tgtgcggttc
     3061 tgggcattgc gatcatcaat attacaaaca actacaatgt gatggagtca ttcaccgcag
     3121 ggttggctgc gggactgggc ttcacattgg cgatcgtatt gatgagcaat gtccgtgagc
     3181 gacttgatct gcatccggtg cgaaaagcgt accgcgggat gccgatcgcc ttcatcagtg
     3241 ccggactcat ggcgcttgcg ttcatggcgt tcgacaaatc actgttgacg aatctgcact
     3301 tggtatagga ggattgtgtg aatattcttc tagcaataat cattgtagcc gtcttgggcg
     3361 gtatatttgg atacggtctc tcatatgcgg aaaagaagct ggcgattgaa aaagatccga
     3421 aagtgcttgc ccttgagccg atcatgcccg gagcaaactg cggaatatgc ggatacgccg
     3481 gatgcaacgc gtatgtggcg gcggtcgtca aaggtgaggc ggaacttggg ttgtgtactc
     3541 ccggcggtcc ctcgcttgtc gaaaagatga gcgagatcat gggtctgaaa gtagatcccg
     3601 tcggtgaggt gaggcgaatg gtcgctcacg tgatgtgtcg ggggaatccc gaggtcacga
     3661 agcgggatta tcggtatgaa ggtcttgagg actgcaacgc tgcggcgctg ctgctcggcg
     3721 gggatacgac gtgcaaatcc gcctgcctgc gcctcggttc ctgcatcaat gtctgtccga
     3781 ccggcgcgat ctccaaggat gaaaaagggt atatcatcgt cgatgaaaaa aaatgcatca
     3841 gctgtgaaaa gtgtgttctg gtgtgtccca ccggtgcgat gcagatgatc ggagaggatg
     3901 atgaatacgc aatcgaatgc aataaccatg aacccggagg aaaggtgagg aaatactgta
     3961 ctgtcgggtg tattggatgt aagatatgtg aaacgaagta tcctgagtcg ggatgtacgg
     4021 ttgatcgctt tttggcgacg ttcaaccagc tggccgaaca caaccagatc gctgatgcgg
     4081 ccgaggcatg tcccaccaag tgcatcgtta agctataggg atgaaacggt tagtcggtgg
     4141 atatactcaa ctggctcatg gtcttccggc gcctttgttt atcagggcgc tggaagcggt
     4201 gttgcttcca ctgctgaccg aactcaaccg aagcgag
//
LOCUS       DAOPUZ010000038         4194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_21370_length_4194_cov_3.279778, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000038 DAOPUZ010000000
VERSION     DAOPUZ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21370_length_4194_cov_3.279778"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PKN79_01275"
     CDS             complement(<1..473)
                     /locus_tag="PKN79_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ94424.1"
                     /translation="MKKGMVFVMIFLLVGSMLFGAGRAEEAAKETKIGVSIMELSAYT
                     WYLGVIDGCKQWAADNPDANFTFQFEDSRSDVSTMLNNIDALITGGAKGIILFPADAS
                     SAIPTMKQYVSQGIPFVIGDYAQDPKSPNDIVWETFVGHDMRMLGETAGEVAVEYL"
     gene            complement(534..1004)
                     /locus_tag="PKN79_01280"
     CDS             complement(534..1004)
                     /locus_tag="PKN79_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNZ94425.1"
                     /translation="MCIASSSRLVWGGVKNIYLIPHHQTEAGALMPMTISCHKAAKKV
                     TMEYMEATKGVGWWGSNDYQDYYENLGSGDDPFSYIKVIPLISKEAQIACGGFDHAGK
                     WETSLMMGTYPDHVDLTRCKLNTEWFAQSAVEATAEIGRHMVNSVLDWLREAIV"
     gene            complement(991..1257)
                     /locus_tag="PKN79_01285"
                     /pseudo
     CDS             complement(991..1257)
                     /locus_tag="PKN79_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
     gene            1493..2674
                     /locus_tag="PKN79_01290"
     CDS             1493..2674
                     /locus_tag="PKN79_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ94426.1"
                     /translation="MLQKRINVGLVISLAGFAISVNTLPPLIEWFSGHLCMPISSFGL
                     VYFFQYGAFTLCSIGIGKLHAKRGLPLLSIAIGALFFSALCVFLIGRATSFILLITLM
                     MLIGGAGGLVESIGTTLLTVEDGSNRMLHASQLFYAIGAVAAPAMVGFLLASGVHVPK
                     ISQYIGIFSLLIAIVVFGLTRSSNSKTLKETPTPSHRPATAPLFALYVLFITMGAYVI
                     VEMSLGNWLAVFAIQTFSVTEAQGSLALSLFWTGLCLSRLFYVLKSVKSQQKTLSIHM
                     ALMLLSCLILIASTKFGTLTLLYPTVILFGIGCGPIWPLLIEFCSAAFREEHLVMYLV
                     ASGSTGALLGPILTSTLFGFFSIRNIPVILSAYTVLMMGSGVLTFRILRNARRTRRSA
                     A"
     gene            complement(2646..3674)
                     /locus_tag="PKN79_01295"
     CDS             complement(2646..3674)
                     /locus_tag="PKN79_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ94427.1"
                     /translation="MSTKKNNKVRLVDIARETGFSIKTVSRVVNNQGDVSPKTRQAIM
                     KVVNKYNYSPNPMAATLRTRRTRTIGLVVPDFTNEFFGEVGLAIEAALRAQSYSLIVS
                     FSGNSHEGEVRALELLSSKYVDGILLASVGTTTDAINELLKEEMPLVVFDNEPPQVAT
                     NVVLHDNINGAYVLTRHLIDHNYRSIGCVAGLQHQSSGSERLEGFLKAMEDNRLEAPA
                     SHIIVEDWTSEGGYRAMNQLLGLPRSERPRAVFISNSIMALGCYKALIQAKVKIPEEM
                     ALVSFDNLSFMESLEPPITTLSSTGHSVGEQAAKLLLATLSNEDSPPVERITIQGTII
                     KRRSCGCA"
     gene            complement(3671..>4194)
                     /locus_tag="PKN79_01300"
     CDS             complement(3671..>4194)
                     /locus_tag="PKN79_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94428.1"
                     /translation="YLGHVNFNYPKSGRLEYSIANFTDREVVVLDETFEGVTKKPELF
                     LTLDKGCTLDPELVAVIDYGKKSPSTFRNGKFALSRMSDEKGGSTWVVARTDILDHTV
                     IWLTQTPDRKAAGFALPATSGPTGFTNEKKMGNGKVLPGKQSITLWYAFGVGDGDIEK
                     DIATALSRSGMAE"
BASE COUNT          842 a         1206 c         1077 g         1069 t
ORIGIN      
        1 aggtactcaa cggccacttc tccggcggtc tcgccgagca ttctcatatc gtgtccgaca
       61 aaggtctccc agacgatgtc atttgggctc ttcggatctt gagcatagtc gccgatgacg
      121 aacggaatac cttgactgac gtactgcttc atcgtcggaa tcgccgagga tgcgtccgcc
      181 gggaacagga tgattccttt tgctccaccg gtgatcaggg catcgatgtt gttcagcatc
      241 gtcgatacgt ccgagcggga gtcctcaaat tggaaggtaa agtttgcatc cggattatcg
      301 gcagcccact gcttacaacc atcgatgact ccgaggtacc atgtatacgc tgaaagctcc
      361 atgatactca ctccgatttt tgtctccttg gcagcctctt ccgctcgccc ggcaccgaac
      421 agcatcgagc cgaccaagag aaaaatcatc acaaaaacca ttcctttttt catacttttt
      481 ctccttatat ggtttttatt tcttcactac tcctttgcag aaaaggaata atgttacact
      541 atcgcttccc gcagccaatc cagcacggaa ttgaccatgt gtctgccgat ttccgccgtg
      601 gcttcgacgg cgctttgggc gaaccattcg gtgttgagct tgcagcgcgt gaggtcgaca
      661 tggtcgggat aggttcccat catcagcgac gtctcccact ttcccgcatg gtcaaaaccg
      721 ccgcaggcga tctgggcttc cttgctgatg aggggtatca cttttatgta tgagaaggga
      781 tcatcccccg agccgaggtt ttcataatag tcctgatagt cgttgctccc ccaccagccc
      841 actcctttcg tcgcctccat atactccatc gtgactttct tcgccgcctt gtggcaggag
      901 atcgtcatgg gcatcaacgc cccggcttcc gtctggtggt ggggaatgag atagatgttt
      961 ttcacgccgc cccagaccaa gcgacttgag gatgcaatac acatattgcg tgtgggtgtc
     1021 ttcatcgacg tgaatcgtgc cggtctcggg acctcccacc gcataggaag cgaccccgta
     1081 ccagatcggc ggcagcacga cgatctcttt ttcttttcca agctcccgca gacatccggt
     1141 aacgaccatc gtatcggttc cgctggaaac atggtgagcg tggtattcaa tcgtcccgac
     1201 gggaatgatg aaggggactc gacgttgttt tgcatcttcg atctgatcaa agtacatgtc
     1261 aattaaatcc attcccacct ccttctcttt cgcagcacaa acctgatatt ccggtaagca
     1321 cgtacaaatc aacaaactcc gtacgtgtaa ttgtacgacc acaagattaa gaaatcaaga
     1381 aaaacattac gtttaacttg aactataatt taaacgttta agcttttact ctgattcttc
     1441 ttgacatcat cggcaggata gtgaattgtt tatcttgtat ggagaaaaat acatgctgca
     1501 gaaaagaata aacgttggat tagtcatcag ccttgccgga tttgcgatat cggtgaatac
     1561 gctgccgccg ttgatcgagt ggttctcggg ccacctctgc atgccgatct cttcgtttgg
     1621 attggtgtat ttttttcagt atggcgcctt caccctctgc tcgatcggga tcggaaagct
     1681 tcacgccaag cgggggcttc cactcctgtc catcgcgatc ggagcgctct tcttttccgc
     1741 tctttgcgtg tttctgatcg gcagagcgac cagtttcatc cttctcatca ctttgatgat
     1801 gctcataggt ggtgccggcg gcttggtgga gtcgatcgga acgacgctgc tcaccgttga
     1861 ggacggatcg aacagaatgc ttcatgcgtc ccagcttttt tatgcgatcg gcgcggtggc
     1921 cgcccccgcg atggtggggt ttcttctggc atccggcgtg catgtgccga aaatcagcca
     1981 atatatcgga atcttttcgt tgctgattgc catagtcgtg ttcggcctga ctcgttcttc
     2041 gaacagcaag acactcaagg aaacaccaac cccctcgcat cgccccgcca cagccccgct
     2101 ttttgccctg tatgtactct ttatcacgat gggcgcctac gtcatcgtcg aaatgtcctt
     2161 gggaaactgg cttgcggtgt tcgccatcca gaccttctcg gtcaccgaag cccaaggatc
     2221 gctcgccctc tcgctcttct ggacggggct ttgcctctct cgattgtttt atgtcttgaa
     2281 gagcgtcaaa tcccaacaaa aaacgctgag catccacatg gcgctcatgc tcctttcatg
     2341 cctgatcctc atcgcatcga cgaaattcgg gacgctgacc ctcctctacc cgacggtcat
     2401 cctctttggc atcgggtgcg gaccgatttg gccgctgctc atcgagttct gttcggcggc
     2461 cttcagagaa gagcaccttg tgatgtatct ggtcgcctcg ggaagcaccg gagcgctcct
     2521 cggcccgatt ctcacctcga ccctgttcgg cttctttagc atccggaata tcccggtgat
     2581 tctcagcgcc tatacggtgc ttatgatggg atccggcgtc ctgacgttca gaattttgag
     2641 gaacgctagg cgcacccgca ggagcgccgc ttgatgatcg tgccttgaat ggtgatccgc
     2701 tcaaccgggg gagagtcttc atttgacagt gttgcaagca ggagttttgc cgcctgctcc
     2761 cccacggagt gtccggtgct gcttaacgtg gtgatgggcg gttcgagcga ttccatgaag
     2821 ctgaggttgt caaaactgac cagcgccatc tcttcgggaa tcttcacctt ggcctgaatg
     2881 agcgccttgt agcacccgag ggccatgatc gagtttgaga tgaacacagc ccggggacgt
     2941 tcgcttctcg gcagaccgag caactggttc atcgcccggt accccccttc gctcgtccaa
     3001 tcctccacga tgatgtgtga agcgggcgcc tccaagcgat tgtcctccat cgccttgagg
     3061 aatccttcca accgctcgga tccggaactc tgatgctgca gtccggccac gcacccgatc
     3121 gaacgatagt tgtgatcgat caggtgccgg gtgagcacat acgctccgtt gatgttgtcg
     3181 tgcagcacga cgttcgtcgc cacctgcggc ggttcattgt cgaacaccac aagcggcatc
     3241 tcctctttca acagctcgtt gatggcgtcg gtcgtggtcc cgaccgatgc gagcaggatc
     3301 ccgtccacgt atttcgaaga cagcagctcg agcgcccgga cctcgccctc gtgggagttg
     3361 ccgctgaagg aaacgatcag gctgtaggat tgggcgcgca gcgcggcctc gatcgcaagg
     3421 cccacttcac cgaaaaactc gttggtgaaa tccggaacga caaggccgat cgtccgggtc
     3481 cgccgggtcc gaagcgtcgc cgccatcggg ttcgggctgt agttgtattt gtttacgacc
     3541 ttcatgatcg cctgccgggt cttggggctc acgtccccct gattattgac gacccgtgaa
     3601 acggtcttga tggaaaatcc cgtttctctg gcgatatcga ccaatcggac cttgttgttc
     3661 ttcttggtgc tcattccgcc attccactcc ttgagagcgc ggtggcgata tccttctcga
     3721 tatcgccgtc tccgactccg aaggcgtacc agagggtaat gctctgtttt ccgggcaaga
     3781 ccttgccatt gcccatcttc ttctcattgg tgaacccggt gggcccgctg gttgcaggaa
     3841 gcgcgaaccc tgcagctttc cggtcggggg tttgcgtcag ccaaatgacc gtatgatcga
     3901 gaatatcggt gcgggcgacc acccaggtgg aaccgccttt ctcatcgctc atccgactga
     3961 gggcgaactt cccgttcctg aacgtggaag gagatttttt gccgtagtcg atgaccgcaa
     4021 ccagctcggg atcgagcgtg caacctttat cgagggtcaa aaacagttcg ggtttcttcg
     4081 tcaccccttc gaacgtctca tcaagcacga cgacttccct gtcggtgaag ttggctatcg
     4141 aatactcgag gcgaccgctt ttcggatagt tgaagttcac gtgaccgaga tact
//
LOCUS       DAOPUZ010000039         4181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_21453_length_4181_cov_3.035870, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000039 DAOPUZ010000000
VERSION     DAOPUZ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21453_length_4181_cov_3.035870"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PKN79_01305"
     CDS             complement(<1..902)
                     /locus_tag="PKN79_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HNZ94429.1"
                     /translation="MALTPSLTVTQKQQLKLTPQLIQSFELMTLPLADLQQRIKEEIA
                     TNPALEIPSTRELSYETVADRKVESELRRVDDSYGDTSTYGFDDDATERVHRVLEQVL
                     TAEETLAEHLIDQLRLERLTEEELEIGLRIITNLDSNGFFIYEPELFIDETERESYEK
                     VLSLLHTFDPVGIAVPSWRESLIVQAAYQGLEGETLQQFTALVMDNLELMRANKLEQV
                     AKNLGISEEELGELYAFLKTLTPFPGQGFASGPQHYVIPDLSIRVEDGALQLRMNEDA
                     LPDIRINPEFEALASDPDAKTCIQ"
     gene            complement(902..1636)
                     /gene="lptB"
                     /locus_tag="PKN79_01310"
     CDS             complement(902..1636)
                     /gene="lptB"
                     /locus_tag="PKN79_01310"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNZ94430.1"
                     /translation="MGNSLIVSNLSKRYGRRLVVDDLSFSMRSGEIVGLLGPNGAGKT
                     TTFYMIVGFLKVRKGSIILNGEDVTGLPMHERSKKGLSYLPQEPSIFRKLSVRDNILL
                     VAQSRNDLTRHEKHHLVDQLVEEFGLTHVVRQKGYTLSGGERRRCEIARALAASPTFL
                     LLDEPFAGIDPKAVYEIKLLIKKLASQGIGILLTDHNVRDTLAVTTNAHIINDGSILV
                     SGTKNELLAHELARKIYFGDQFEGEL"
     gene            complement(1629..2252)
                     /locus_tag="PKN79_01315"
     CDS             complement(1629..2252)
                     /locus_tag="PKN79_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94431.1"
                     /translation="MKRLFFLILLIGFFLASASGNDITFGGGNTSIQTQAGRQVITLS
                     QGAHIQSGSIILKADTITLYGDGFRYVSCQGGLSLVDEERNLKLKANRLFYDREAEEI
                     LIEGYVELDDPANELIARGFGLEYRIDEQRISLHAEVTLEKHTSSGAMVCKADVMSFD
                     RTAMSLLLSGKASVHWAGDRYEAQQIVIDLEREEITAEGSIRGVIHG"
     gene            complement(2249..2788)
                     /gene="lptC"
                     /locus_tag="PKN79_01320"
     CDS             complement(2249..2788)
                     /gene="lptC"
                     /locus_tag="PKN79_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNZ94432.1"
                     /translation="MMKRILGMVLASLLLLSCALEPTIQSPVDSSKREADLTLYDATY
                     TFGMSNGTPLAVTAGVLAFHEEEQEAVLEHVTFIGYEEDGKPFFQGSARHAVINTTTY
                     DATLSEKVSLQHLNEGIQVFSDRLSWSDKSQQLSSPGDIASTIIIDGDITLRGEGLMI
                     DFTTLSVTFKKVLEGRVSR"
     gene            complement(2826..3236)
                     /locus_tag="PKN79_01325"
     CDS             complement(2826..3236)
                     /locus_tag="PKN79_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNZ94433.1"
                     /translation="MLEVVTVQDIRSKDRIRVSCSSAACQGCKGGLFCNTKKRVFDVS
                     NIDGLAIEKGDRVEIHLKTGRTIGGTLLTLIAPLLLFPLGYYTAKSLGAGEGICTLIA
                     LGSIALGFVGVWIYFKVNEHSYLPTVKRVIKDAE"
     gene            complement(3239..4033)
                     /locus_tag="PKN79_01330"
     CDS             complement(3239..4033)
                     /locus_tag="PKN79_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNZ94434.1"
                     /translation="MSIRVLFLGEIVGKAGVQAVRQNLRTLREEQAIDLVIANGEGAT
                     GGFGLGKAHSMQLLKSGIDVLTGGEKIYYKLDMVEFIDKNPAILRPANFPQQNPGRGV
                     RYLNVGETKICVINILGNADFPRTHLTNAFPLAEYLVTKALEEGAIPLVQFHASPTAE
                     KQAMGFHLAGKAAAVIGTHSKVLTSDEQILDGGTAFISDNGRCGSQMSVGGFEPEAEI
                     EKQITQLPVRSKEYWEDTVLVGVIVEIDETGKATAIEPIRSALKEE"
BASE COUNT          884 a         1137 c         1181 g          979 t
ORIGIN      
        1 tcctgaatac atgtcttcgc atccgggtcc gaggcaagcg cttcaaactc gggattgatc
       61 cggatatccg gcagcgcatc ctcattcatt cgcaattgca gcgccccgtc ttcaaccctg
      121 attgaaaggt cggggatgac gtagtgctga ggaccggagg cgaacccctg gccggggaag
      181 ggggtcaggg tcttcagaaa cgcatagagc tcaccgagct cctcttcgct gatcccaagg
      241 ttcttcgcca cctgctcgag cttgttggcg cgcatcaatt ccagattgtc catcacgagc
      301 gcggtgaact gctgcagcgt ctctccttcg agcccttgat aggccgcctg aacgatcagg
      361 gactcccgcc acgagggaac ggcgatgccg accggatcga aggtgtgcag gaggctcaga
      421 accttttcat aggattcacg ctccgtctca tcaatgaaca gctccggttc atagatgaag
      481 aatccgtttg agtcgagatt ggtgatgatt ctcagaccga tctccagctc ctcttcggtt
      541 aagcgctcaa ggcgcagttg atcgatgagg tgctcagcca acgtctcctc ggcggtgagc
      601 acttgctcga gaacccggtg aacccgttcg gttgcgtcat catcaaagcc ataggtactt
      661 gtatcgccat aggaatcatc gacccggcgg agctcgcttt ccaccttcct gtcggcaacc
      721 gtttcgtagg agagttcgcg ggtggatgga atctccaagg cggggttcgt agcgatctcc
      781 tctttgatcc gctgctgaag gtccgccaga ggaagggtca tcagctcgaa ggattgaatc
      841 agctgaggag tcagcttgag ctgttgtttt tgggttacgg tcaatgaagg agtcaacgcc
      901 atcagagttc cccctcgaac tgatcgccga agtagatctt tcgggccagt tcatgggcga
      961 gcagctcgtt tttcgtaccc gagacgagga tcgaaccgtc attgatgata tgggcgttgg
     1021 tggttacggc gagagtgtcg cggacgttgt ggtcggtcag gagaatgccg attccctggc
     1081 ttgcaagctt tttgatcagg agtttgattt catagaccgc cttggggtcg atgccggcga
     1141 acggctcgtc gagaagcaaa aacgtcggac tggcggcaag agcgcgggcg atctcacagc
     1201 ggcgccgctc gcctccgctg agggtatatc ctttttggcg gaccacgtgc gtcagtccga
     1261 actcctcgac cagttggtcg acaagatggt gtttttcatg tcgagtcaaa tcgttccgac
     1321 tttgagcgac aagcaggata ttgtcccgga ccgaaagctt tcggaaaatc gagggctcct
     1381 gaggaagata ggaaagtcct ttttttgagc gctcatgcat cggcaaaccc gttacatcct
     1441 ccccattgag gatgattgaa ccctttcgga ctttgaggaa tcccacaatc atgtagaacg
     1501 tggtggtttt tcccgctccg ttgggaccga ggagaccgac gatctctccg gagcgcatcg
     1561 aaaaggagag gtcgtctacg accagccggc gaccgtatcg tttggaaagg ttggagacga
     1621 tgagggagtt acccatggat cactccccgg attgaaccct cggcggtgat ctcttcccgt
     1681 tcaaggtcga ttacaatctg ctgcgcttca tatcggtcgc ccgcccaatg aacgctggct
     1741 ttaccgctga gaagcaggct catcgccgtt cgatcgaagc tcatcacatc cgctttgcag
     1801 accatcgctc cgcttgaggt gtgtttttcc agcgttactt ccgcatgtag cgagatccgc
     1861 tgttcatcga tccggtattc aagaccgaac cctcgggcga tcagctcatt ggccggatcg
     1921 tccagctcga catacccctc aataagaatt tcctccgcct ctcgatcata aaagagacgg
     1981 ttcgctttca atttcaggtt tcgctcttca tcaacgagtg aaagacctcc ctgacaggag
     2041 acgtagcgga atccatctcc atagagggtg atcgtgtccg ctttgaggat gatcgaaccg
     2101 ctttgaatat gagcaccttg gcttaaggtg atcacctgtc gtcccgcttg cgtctgaatc
     2161 gaggtgttcc ccccgccgaa cgtgatatcg tttccgcttg ccgaagcaag gaagaacccg
     2221 atgagaagga tgaggaaaaa gagtcgtttc atcggctcac cctcccttca agaaccttct
     2281 tgaaggtgac ggaaagggtg gtgaaatcga tcatcaaccc ttcgccgcga agggtgatgt
     2341 ccccatcgat gataatcgtt gaagcgatgt cccccgggga ggacagttgc tggcttttgt
     2401 cagaccagga aagccgatcg ctgaagacct ggatcccctc attgaggtgt tgaagggaga
     2461 ccttctccga aagggtggcg tcgtaagtgg ttgtgttgat gaccgcatgc cgggcggaac
     2521 cttggaaaaa cggtttcccg tcctcttcgt aaccgatgaa ggtgacgtgt tccagcacag
     2581 cttcctgttc ttcttcatga aatgcgagca ccccggcggt gacggcaaga ggggtaccgt
     2641 tcgacatgcc gaaggtgtac gtcgcgtcat agagagtgag gtccgcttct cgtttgctcg
     2701 aatcgaccgg gctttggatc gtcggttcca gggcacagga aaggagcagg agagaggcaa
     2761 gaaccatgcc aaggattcgc ttcatcatgg tttgagtata cctttccggg tgagcttggg
     2821 caatcttatt cagcgtcctt gatgacacgt ttgacggtgg gaaggtagga gtgctcgttg
     2881 accttgaaat agatccacac ccccacgaat ccgagggcga ttgagccgag ggcgatgagc
     2941 gtgcagatcc cttcgccggc gccgaggctc ttggcggtgt agtagccaag cgggaagagc
     3001 aggagcgggg cgatgagggt aaggagcgtc ccgccgatcg tccggccggt tttcaggtga
     3061 atctccactc gatccccttt ctcgatcgcc agaccgtcga tgttcgagac atcgaagacc
     3121 cgtttcttcg tattgcaaaa aagaccgcct ttgcatcctt ggcacgccgc tgacgagcag
     3181 gagacccgga tgcgatcctt tgagcggata tcttgaacgg taaccacttc caacacggtt
     3241 actcctcctt cagggcagaa cggatcggct cgatcgccgt cgccttgccg gtctcgtcga
     3301 tctcgacgat tactccaacc agaacagtgt cttcccagta ctctttgctg cgaaccggca
     3361 gctgggtgat ctgtttttca atctcggctt ccggttcgaa tcccccgacg ctcatctggc
     3421 ttccacagcg tccgttatcg ctgatgaagg cggttccccc gtcgaggatc tgctcatcgg
     3481 aagtaagcac cttggagtgg gttccgatga ccgctgccgc cttgcccgcc agatgaaacc
     3541 ccatcgcctg cttctcggca gtgggagaag cgtgaaattg cacaagggga atcgccccct
     3601 cttcaagggc tttcgtcacg aggtattcgg ccagcgggaa cgcattggtc aaatgggttc
     3661 ggggaaaatc ggcgttgccc aggatgttga tgacgcagat tttggtttca ccgacgttca
     3721 gatagcgaac tccccgaccg gggttctgct gggggaagtt tgcaggtcga aggatcgcag
     3781 ggttcttgtc aatgaactcg accatgtcaa gcttatagta gatcttctcg cctccggtca
     3841 gcacgtcgat gccgctcttc agcagctgca tcgaatgggc tttgccgaga ccgaatcctc
     3901 cggtggctcc ttccccgttt gcgatcacca gatcgatcgc ttgttcttcc cggagcgttc
     3961 tgaggttttg gcgaacggcc tgaactcccg cctttccgac gatttctcct aaaaaaagga
     4021 cccgaatact catggttcaa aggataccga atcggatgga aaaagtacac gcctgcaaaa
     4081 aaggagactg aatgaccagt ctccctgctt tgagtccgac gcgattcgaa cgcacggcct
     4141 ttcgcttagg agatcgaaaa agcgttcgcc tttgctgttc c
//
LOCUS       DAOPUZ010000040         4153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_21630_length_4153_cov_3.475842, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000040 DAOPUZ010000000
VERSION     DAOPUZ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21630_length_4153_cov_3.475842"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PKN79_01335"
     CDS             complement(<1..162)
                     /locus_tag="PKN79_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNZ94435.1"
                     /translation="MNKPSILWYDLETFGLDSRGDRIAQFAAIRTDLDLRQIEEPTIL
                     YCKLSDDYLP"
     gene            229..840
                     /locus_tag="PKN79_01340"
     CDS             229..840
                     /locus_tag="PKN79_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94436.1"
                     /translation="MNRQTIGLVLILLLVIAPLTAAKPSERDILIAVTAISDATIANV
                     AAYLNTPALNLPGSIFEKEARATLPKALELKDADLGIYRKTYQSLNKPQSNFLLSLLQ
                     SAKGPLNDVALLFLDTHEWEEGQVSLTGRVSTVWGEGVTLASLMTSVVTGGAIDPIEA
                     IVDVTAAGTRLSTDVSISGSFLLFTDQEGYFVIEPRELKVNGE"
     gene            844..2157
                     /locus_tag="PKN79_01345"
     CDS             844..2157
                     /locus_tag="PKN79_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexokinase"
                     /protein_id="HNZ94437.1"
                     /translation="MHELIKKTEEFLNRSKIDADQVDMDQLITSFLSEMEKGLAGAPD
                     SSLKMIPTYTTTSETILADQPVIVIDAGGTNLRTCLVRFDREKNPRISEFKKTSMPGV
                     KSEVTAKEFFSIIADEVERLIDRSDRIGFCFSYAAEITSDRDGIPLVFSKEIKAPEVI
                     GMPLGKSLLAELASRGHDVRKKKVAVLNDTVATLLAGQGTPSDVPYSGYIGFILGTGT
                     NTAYVESNKKITKLSADPSKAQIINIESGNFAYCPGPFDRKFFDSTKEPNQYHLEKMI
                     SGAYLGPICGSIITEAADAGLFSETFKERYAKRIKTLSTTVMSNYLEMPFNRDYELVQ
                     CVEGNEGDARVLWLIINAVIERAAKLTAANLTSTILKSGSGTDPRKPVCINADGTTLY
                     KTEYLLEYTNFYLHHYLQRKYKRYYRFVRIDDSPTIGSAVAALGL"
     gene            complement(2158..4005)
                     /locus_tag="PKN79_01350"
     CDS             complement(2158..4005)
                     /locus_tag="PKN79_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HNZ94438.1"
                     /translation="MIIHDTLNDQAIADWRTNPLAFPLPSKESLSDAYTSMIISSSGW
                     RKVFAPGGDEEDSGRTVNPPDRILAALAAYALYRHVGKKKPTILVGLDARPTGAHLGS
                     IAVYTLLSLGAKVRYLFITAAPQIMADTNLHPKEADAFLYISASHNPIGHNGFKFGKE
                     GGVYGSKDAERLAELFRELVDDGLTHFALLQTYGENLDPQIYKRTLERVGEERKKSAE
                     RYRTLALRIATKSDDEQAHTRFIETMRIKHQQKPLGILGELNGSARSCSIDRPLLSEL
                     GITTEFMHDTPGDVVHPIVPEGKNLTLCMESLTALHQKNPAFVLGYVPDNDGDRGNIV
                     YINDRKTATILEAQSLFALIVLSELAQSRLSDPDAPLAVVVNGPTSNRVDEIARSFGA
                     KVFRAEVGEANVVELSNLKRKEGYVVPILGEGSNGGNISHPAKVRDPLNTLLSLTKLL
                     KQEELSRLWFTLSGQPQPASLTLDQLIRSLPSYITTGAYSSLAQLSVNIEHGRLKTRY
                     EEFFEEIFNRDHILFNSFGITSYKIIQTEGTEERIGSGPAYRTPPYNGGFKVGLYNDE
                     NVMTDFLWMRGSKTEPIFRLLVDCKGDNTNRHDYLLELHRLVITRALQS"
BASE COUNT          965 a         1086 c         1154 g          948 t
ORIGIN      
        1 ggggaggtaa tcatcgctga gcttgcagta gaggatggtc ggctcttcga tctgccgaag
       61 atccaaatcg gttcggatcg cggcaaattg ggcgattcgg tcccctcggc tgtcaaggcc
      121 gaatgtttca aggtcatacc aaagaattga cggtttattc attgcttcag tgtatcacac
      181 ctcttcttca ttgtcttaat cgattcttcc ctttatgata cgtacaagat gaatcgtcaa
      241 accattggat tagttctcat tcttctcctc gtcattgccc cgctcacggc agcaaagccg
      301 agtgaacggg acatcctgat cgctgtcacc gcgatcagcg acgccacgat cgccaacgtc
      361 gccgcgtatc tcaatacccc cgcgttgaac ctgcccggat caatctttga aaaagaggcg
      421 agggccacgc tgcctaaagc gctcgagctg aaggatgcgg atctcggcat atatcggaag
      481 acgtatcaat cgttgaacaa gccgcaaagc aactttctgc tctcattgct tcaaagcgcc
      541 aaaggtccgt tgaacgatgt ggcgcttctc ttcctcgata cccatgaatg ggaagaggga
      601 caggtttcgc tcaccggacg ggtgagcacc gtttggggag aaggagttac cctggcgagt
      661 ctgatgacca gcgtggttac cggaggagcg atcgatccga tcgaagcgat cgttgatgta
      721 acagccgccg gtacccggct gtccaccgat gtatcaatca gcggctcgtt cttgcttttt
      781 accgatcagg aaggatattt tgtaatcgag ccaagagaac tgaaagtaaa cggagaataa
      841 acaatgcacg agctcatcaa aaagaccgaa gagtttctca atcgctccaa gatcgatgcc
      901 gatcaggttg atatggatca gttgatcaca tcgttcttgt cggagatgga aaaaggtctg
      961 gccggtgctc ctgattcgtc actgaagatg attccgacct ataccacgac aagtgaaacc
     1021 attctggccg atcagccggt catcgtgatc gatgccggag ggaccaacct tcggacttgt
     1081 ctcgtccggt ttgatcggga gaaaaacccc cgaatctcgg agttcaaaaa gacctcgatg
     1141 ccgggggtga aaagcgaagt gactgcgaag gagttctttt caatcatcgc cgacgaggtc
     1201 gaacgactca ttgatcggag tgatcgaata gggttctgct tctcgtacgc cgctgaaatc
     1261 acctccgacc gggatggaat tcccctggtg ttctccaaag agatcaaagc tcccgaagtg
     1321 atcggtatgc cgcttggcaa gagtctgctt gccgagctgg caagccgcgg acacgatgtg
     1381 cggaagaaaa aagtcgccgt attgaacgat acggtggcga cgctgctcgc cggtcaggga
     1441 actccgagtg atgttcccta cagcggctat atcggcttca ttctgggcac ggggacgaac
     1501 accgcctatg ttgagtcaaa caaaaagatt accaagcttt ccgccgaccc ctccaaagcc
     1561 caaatcatca acatcgaatc gggaaacttc gcctactgtc ccggcccctt cgaccggaag
     1621 tttttcgatt ccaccaagga gcccaaccaa taccacctcg aaaagatgat cagcggcgcc
     1681 tatctggggc cgatctgcgg ttcgatcatc accgaagcgg ccgatgccgg gctcttttcc
     1741 gagacattta aagagcgcta tgcaaagcga atcaagacgc tttcaaccac cgtaatgagc
     1801 aactacctcg agatgccgtt taatcgcgac tatgaacttg tccagtgtgt tgagggcaat
     1861 gaaggcgatg cccgcgttct ttggctgatc atcaacgccg tcatcgaacg ggcggcgaaa
     1921 ctgaccgcgg cgaatctcac atcaacgatc ctcaagagcg gatcggggac cgacccccgg
     1981 aaaccggtgt gcatcaacgc cgacggaacg accctctata aaaccgaata ccttcttgaa
     2041 tacacgaact tctacctcca ccactacctg cagcggaaat acaagcgtta ctaccgcttc
     2101 gtccgaatcg atgattcacc gacgatcggc tcggccgtgg ctgcgttggg gctgtaacta
     2161 cgattggaga gctcgggtga tgacgaggcg gtgaagctcc aaaagataat cgtgccgatt
     2221 cgtgttgtca cccttgcagt cgacgagtag gcggaagatc ggctccgtct ttgaaccccg
     2281 catccacaga aaatcggtca tcacgttctc atcgttgtac aacccgactt tgaagccgcc
     2341 gttgtacgga ggagtgcggt atgccgggcc gcttccgatc cgctcttcgg ttccttccgt
     2401 ttgaatgatc ttgtaggagg tgattccaaa gctgttgaac aggatgtgat cacggttgaa
     2461 gatctcttcg aagaactcct cgtaccgggt cttcagccgg ccgtgttcaa tgttcacgct
     2521 cagctgcgcc aaagaggagt atgctccggt tgtgatatag gaaggaaggg aacggatcaa
     2581 ttgatcgagc gtcaacgagg cgggttgcgg ttgtcccgag agggtgaacc acaacctgct
     2641 tagttcctct tgtttcaaga gtttggtcaa agagagcagg gtgttcaacg ggtcgcggac
     2701 cttcgccggg tgggagatgt tccctccgtt cgagccttca ccgaggatcg gaacgacgta
     2761 tccctctttt cgcttgagat tagaaagctc cacgacattc gcttctccca cttcggcgcg
     2821 aaataccttc gcaccgaacg agcgggcgat ctcatcgacg cgattggagg tcggtccgtt
     2881 cacgacgacc gccagggggg catcgggatc ggaaagtcgg ctttgggcga gctcggagag
     2941 aacaatcaac gcaaagaggg attgagcctc cagaatcgtc gcggttttcc tgtcgttgat
     3001 gtagacgata tttccccggt ctccgtcatt gtcggggaca tacccgagga caaacgcggg
     3061 gttcttctga tgaagggctg taagcgattc catgcagagg gtgaggttct tcccttcggg
     3121 aacgatcggg tgaaccacat ccccgggggt gtcgtgcatg aattcggtgg tgattccgag
     3181 ctccgaaagc agggggcgat cgatcgagca gctacgggcc gagccgttca gttcgccgag
     3241 gattccaagc ggcttctgct gatgtttgat tctcatcgtt tcgatgaacc gggtatgagc
     3301 ctgctcatca tcgcttttgg tcgcgatcct caaagcaagg gtgcgatagc gctctgcgct
     3361 ttttttccgt tcttcgccga cccgctcaag ggttcgttta tagatttgag gatcaaggtt
     3421 ctctccgtat gtctgaagaa gggcgaagtg ggtcagaccg tcgtcaacca gctcacgaaa
     3481 gagctcggca aggcgttcgg catccttgga gccatataca cccccttctt ttccgaactt
     3541 gaacccattg tggccgatag ggttgtggct ggctgagatg tagagaaatg catcggcttc
     3601 tttggggtgg agattggtat cggccatgat ctggggcgct gcggtgatga agaggtagcg
     3661 aacctttgcc ccaagggaaa ggagggtata aaccgcgatg gagccaagat gggctccggt
     3721 tggacgggcg tcaaggccta cgagaatggt tggcttcttc ttgcccacgt gccggtagag
     3781 ggcatacgcc gccaacgccg ccagaattcg atccggagga ttgaccgtcc ggccggagtc
     3841 ttcttcatct ccgccgggag cgaacacctt ccgccaaccg gaggaggaga tgatcatgct
     3901 cgtatatgca tcgctcaagg attccttgga aggaagcggg aatgccaggg gatttgtccg
     3961 ccaatctgca attgcttgat cattgagagt atcgtgtata atcatactct tgagtgtagc
     4021 atatattgtc agtaaagagg atgtaggata gggtagaacc atgcaaaaaa catgtgccgc
     4081 aattcatgat ctcgcctcat atgcaaaaag ctcgctgacg gtcgtccttc ccgccctcga
     4141 agcgcttggc gtg
//
LOCUS       DAOPUZ010000041         4136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_21739_length_4136_cov_3.564568, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000041 DAOPUZ010000000
VERSION     DAOPUZ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21739_length_4136_cov_3.564568"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKN79_01355"
     CDS             <1..382
                     /locus_tag="PKN79_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional NADH-specific enoyl-ACP
                     reductase/trans-2-enoyl-CoA reductase"
                     /protein_id="HNZ94439.1"
                     /translation="VSVNKALVTRASAVIPVVPLYMALLYAVMSEQGIHEQCTEQIWR
                     LFTDRLYTGIPVPTDKEGRIRLDDWEMDPLIQAAVQGRWDMQREGKPLKGGNLALVRQ
                     EYDRIHGFGYEEIDYEADVDPRVV"
     gene            complement(349..1701)
                     /locus_tag="PKN79_01360"
     CDS             complement(349..1701)
                     /locus_tag="PKN79_01360"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNZ94440.1"
                     /translation="MNMVSTIVTKRDGWEIQRVQKVEGFTWEELRPQAPPFDSRAIGE
                     IARLYQVTIIPKNPGKFGWFVFFRLDEDLPFPLAMTQGEITFLDPNLAFASYVNGLVS
                     RGEIAINDRLVVSDPVLREYLDRLATKGLLSIAKGTAASLRFIPISDELGFASSVEGA
                     LVVNSHFFLMDSSDLDSPFCTLGSPYGLSVHQGLVGLPPLNERPALFASKAGEAWIEV
                     PKLSDLTIEVDGIPLKGTIHTRREGRSTPPSDNQEIVIVGRKVVGVATGGKTPIPMAG
                     FVVSCKEAIEPGKGIVTYKGYEDILFAIQVGPSMMNDGEIIDSFDCFFYDPKCNDTAF
                     PPTVYPLDFNTGRAARIALGTNNEGKPILLWAEGASKVRHVPGAGSSGASLLELARFC
                     EEEGYGNIVNLDGGGSAQILIDGKRQLKISDRHPSDEEAERPVPRVLVYTTRGSTSAS
                     "
     gene            complement(1698..2318)
                     /locus_tag="PKN79_01365"
     CDS             complement(1698..2318)
                     /locus_tag="PKN79_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNZ94441.1"
                     /translation="MESDVKKPVTLLALDFGGVVVEFIDDGVLEAMSKHTGFDHPTFT
                     ERYWRHRSDFDRDRYTLLEYWRLVTGEENPPIDALVSLDLAGWSRIDPAIIRWAEGLK
                     ERGLKLALLSNMATPTYEALQKDEHWPTFFDYYIISGVLKVNKPDRAIYEHLIAVSDT
                     KPSEILFVDDLMHNIQAAQAAGIHAIRYRGMDDLIRILRKEYPSLV"
     gene            complement(2308..3657)
                     /locus_tag="PKN79_01370"
     CDS             complement(2308..3657)
                     /locus_tag="PKN79_01370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNZ94442.1"
                     /translation="MIRLTKGVFYDLAIWMMGFGMLVRVVFPFFMEFLGMDPSTVYTV
                     WFFSVCIFSGLLVGAVNILLAHWVVGSRLNMLSTHMGKIEDKLHMISGSDTMVDCSAQ
                     DCHLLVDSTDAIGRSSASFNSLVDTLSSSLQLEMQIRSYTRMLTTHLEVEALCQNALS
                     SLLELFNIDGGAVLVEIKGNLKLLGSSGLANGEELGKNPQVLDTLSQLNRKRIDIPVQ
                     AEIDGVIARFRPQTVLLFPIIYKGLGMGVVILAAKHSIPRDVEKNLDLFISSLALALH
                     NAVTHDQIQRLAAVDPLTGVYNRRFGLTRLREEFIRSVKQNTTLGILMIDVDHFKDVN
                     DTYGHTVGDRVLQGIASSARTQLREGDLLIRLGGDEFMVVLLGASGNDVLEVGEAIRR
                     TVSERTTSWGEQQIKVTVSIGGASLATANVKDEQDLIDAADKALYQVKESGRNRVGI"
     gene            complement(3654..>4136)
                     /locus_tag="PKN79_01375"
     CDS             complement(3654..>4136)
                     /locus_tag="PKN79_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNZ94443.1"
                     /translation="VDLHGWLTQAQFIDLITIAEMVPGSMSINSATFIGIKIAGIPGA
                     IVAIVGNILPSFIIMLILAKLYEHFSEQRIIRNVLTSVRPIVVAAILTAALILLEFAV
                     GTFKQPNIFAIIVFLLAYTLLSSKRIPMGPVSVLGVGALVGVAKLGIVYLLEAGIVVI
                     "
BASE COUNT          907 a         1156 c         1205 g          868 t
ORIGIN      
        1 tgtctcggtg aacaaagctc tggtgacccg ggcgtcggcg gtgattcccg tcgttccgct
       61 ctatatggcg ctcctctatg cggtgatgag tgagcagggc atccacgagc agtgcaccga
      121 gcagatttgg cggcttttca ccgatcggct ctataccggc atccccgttc cgaccgacaa
      181 agaagggcgg atccgcctcg atgactggga gatggatccc ctaatccagg cggcggtgca
      241 aggacgctgg gacatgcaac gagaaggaaa acctctgaaa ggagggaatc ttgccctcgt
      301 ccggcaggag tatgaccgga tccacggctt cgggtatgag gagatcgatt acgaggcgga
      361 tgtcgatccc cgggtggtat agaccagcac ccggggaacc gggcgctccg cctcctcgtc
      421 cgagggatgg cggtcggaga tcttcagctg ccgttttcca tcgatcagga tctgggccga
      481 gccccctccg tccaggttga cgatgttccc atacccttcc tcttcacaga agcgggcaag
      541 ctcaagcagg gaagcgccgc tgctgcccgc ccccggcaca tgcctgacct tgctcgcccc
      601 ttccgcccaa agcaggatcg gcttgccttc gttgttcgtt ccaagggcga tccgggcggc
      661 ccggccggta ttgaagtcca gaggatagac ggtgggaggg aaggctgtgt cattgcactt
      721 cggatcgtag aagaagcaat caaaggaatc gatgatctca ccgtcgttca tcatcgacgg
      781 gcccacctgg atcgcgaaga ggatatcctc atatcctttg taggtgacga tccctttccc
      841 cggttcaatc gcttctttgc agctgaccac gaaccccgcc atggggatgg gggtcttccc
      901 tcccgtcgca accccgacca ccttcctgcc gacaatgacg atctcctggt tatcggaggg
      961 cggagtcgat cggccttccc ttcgggtgtg gatcgttcct ttaaggggaa tcccgtcgac
     1021 ctcgattgtc agatcggaaa gcttcggcac ttcgatccat gcttcaccgg ccttgcttgc
     1081 aaacagcgcc ggccgttcat tcagcggcgg aagccccaca agtccctgat gaaccgagag
     1141 tccataggga gaaccgaggg tgcagaacgg agaatcaagg tcggaagagt ccatcaggaa
     1201 gaagtgggag ttcaccacaa gagctccttc tactgatgaa gcgaacccca gctcgtcgga
     1261 gatcggtatg aagcgcaggg aagcggccgt tccttttgca attgaaagaa ggccttttgt
     1321 agccagacga tcgagatatt ccctcaaaac agggtcactg accaccaacc gatcgttgat
     1381 ggcgatttct cctctggaaa ccaatccatt cacatatgaa gcgaatgcaa ggttgggatc
     1441 caaaaaggtg atttcccctt gagtcatggc aagaggaaac ggaaggtctt catccaatcg
     1501 aaaaaagaca aaccaaccaa acttgccggg gttctttgga atgatggtga cctgatagag
     1561 ccgggcgatc tccccgatgg ctcggctgtc aaacggaggc gcttgaggcc gaagttcttc
     1621 ccatgtaaag ccttcaacct tctgaacgcg ttgaatctcc cagccgtccc gcttcgttac
     1681 gatcgtggat accatgttca taccaaggaa gggtattctt tccgcaggat gcggatcagg
     1741 tcgtccatcc cccggtagcg gatcgcatgg atgcccgccg cttgggcggc ttggatattg
     1801 tgcatgaggt catcgacaaa caggatctcg gaaggctttg tgtcactgac ggcaatgagg
     1861 tgttcgtaga tcgccctgtc cggtttgttg accttgagaa caccggagat gatgtaatag
     1921 tcgaagaagg tcggccaatg ctcatccttc tgcagcgctt cataggtcgg agtggccatg
     1981 tttgaaagaa gggccagctt caagccgcgt tctttgaggc cttcagccca ccggatgatc
     2041 gccgggtcga tccggctcca tccggccaag tccagcgaga caagcgcatc gattggcgga
     2101 ttctcttccc cggtcaccaa ccgccagtat tcgaggaggg tgtagcgatc gcggtcgaag
     2161 tcggagcgat gcctccaata gcgctcggtg aaggtcggat gatcgaaccc ggtgtgtttg
     2221 ctcatcgcct ccagcacccc atcatcgatg aattcgacga cgactccgcc gaaatcaagg
     2281 gcgagcaggg tgacgggctt cttcacatca gattccaact ctgtttcttc cgctctcttt
     2341 cacctgatag agcgccttgt cggcggcgtc gatcagatcc tgttcatcct tcacattggc
     2401 ggtcgccagg gaagcgcctc cgatcgagac ggtgaccttg atctgctgct cgccccagct
     2461 ggtggtccgc tcgctgaccg tccgtcgaat cgcttcgccg acctccagaa cgtcgtttcc
     2521 gctggccccc agcaggacga ccatgaattc atctccgccg agccggatca acaggtcgcc
     2581 ttcgcgaagc tgggtgcggg cgctggaggc gatcccctgc aggacccggt cgccgaccgt
     2641 gtggccgtag gtgtcgttga catccttgaa gtggtccacg tcgatcatca ggatgcccag
     2701 cgtggtgttc tgcttcaccg accggatgaa ctcctcgcgg aggcgggtca gcccgaagcg
     2761 gcggttgtac accccggtca ggggatcgac ggcggcgagc cgttggatct gatcgtgggt
     2821 aaccgcgttg tgcagggcaa gagcgaggct cgagatgaag aggtcgaggt tcttttccac
     2881 atcgcggggg atcgaatgct tggcggcaag gatgacgact cccatcccta ggcctttgta
     2941 gatgatggga aacagcagca ccgtttgagg acggaacctt gcaatgactc cgtcaatctc
     3001 ggcttgaacc gggatgtcga tccgcttgcg gtttaattgg ctcagcgtat cgagaacctg
     3061 agggttcttg ccaagctcct caccgtttgc caatccgctg gaaccgagga gtttcaggtt
     3121 ccctttgatt tcaaccaaga ctgccccgcc gtcgatgttg aacagctcaa gcaggctgga
     3181 aagggcgttc tggcagagcg cttccacttc caggtgggtc gtgagcatcc gggtgtagga
     3241 gcgaatctgc atctccaatt gaagggatga ggaaagcgta tcgaccaggc tgttgaagga
     3301 tgccgaagaa cggccgatcg catcggtgct gtccacgaga aggtggcagt cttgggcgga
     3361 acagtccacc atcgtgtcgc tgccgctgat catatgcagc ttgtcttcga tcttgcccat
     3421 atgggtggaa agcatattca gcctgctgcc gacgacccaa tgggcaagga ggatgttcac
     3481 cgccccgacg aggagcccgg aaaaaataca cacactgaaa aaccagacgg tgtacacggt
     3541 cgaggggtcc attcccagga attccatgaa aaagggaaag acgactctga caagcatccc
     3601 gaaacccatc atccagatgg ccagatcata aaacacacct tttgtcagac gaatcatatt
     3661 actactatac ctgcctctag aagatatact atacctaact tggcgactcc gaccagtgcg
     3721 ccgactccca aaacggagac agggcccatc ggaattcttt tggaactgag cagcgtgtat
     3781 gccagcagaa agacgatgat tgcgaagatg ttgggttgtt tgaacgttcc gacggcaaac
     3841 tccagaagaa tcaatgcggc ggtgagaatc gctgcgacca cgatcgggcg aacactcgtg
     3901 agcacgttgc gaatgatccg ctgctcgctg aagtgctcat acagcttggc gagaatcagc
     3961 atgatgatga aggagggcag aatgtttccg acgatggcga cgatcgcgcc gggaatcccc
     4021 gcgatcttga ttccgatgaa tgtagcggag ttgatcgaca tcgagccggg aaccatctcg
     4081 gcgatcgtga tcaggtcgat gaactgggcc tgggttagcc agccgtggag atcgac
//
LOCUS       DAOPUZ010000042         4101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_21984_length_4101_cov_3.293623, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000042 DAOPUZ010000000
VERSION     DAOPUZ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21984_length_4101_cov_3.293623"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1271
                     /gene="xseA"
                     /locus_tag="PKN79_01380"
     CDS             78..1271
                     /gene="xseA"
                     /locus_tag="PKN79_01380"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNZ94444.1"
                     /translation="MDVPTITVTELTRSIKQVLEEGFPFFSVTGEISNFRPASSGHWF
                     FTLKDEGAQIGCVIFRSNAWKVTFTPQDGDKVTITGSLDLYPPRGTYQIKCETIVKTG
                     FGNLLALLEERKTRYQQKGYFDRKRPLPKTPRKLGIITSATGAALQDVLSVLGRRAPS
                     LEILIFPSPVQGAEAAQSIAFRIEQANLLSDCDVLLITRGGGSIEDLLPFSEEAVLEA
                     IHASAIPTVSAIGHEIDWALSDFVADLRAPTPSAAAELISKGIYDSKTELVRIKQRLI
                     AAIEYRLLRAEAAVGKVSVPALRAYLLAQVDRRQFRLANVQLALSNGIDDQLARRRNK
                     LTLLSNQLSALSPLAVLERGFALVSTKEGAVLRSSKPVHDGDRLKIRWIDGERSVRVE
                     EQDEL"
     gene            1261..1479
                     /gene="xseB"
                     /locus_tag="PKN79_01385"
     CDS             1261..1479
                     /gene="xseB"
                     /locus_tag="PKN79_01385"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNZ94445.1"
                     /translation="MNFEEKIAKIETITKKLQDEHTSLEDSIALFEEGVTLAKELEQA
                     LEEAKGKVEQVVGESLASMEVVEFDDEQ"
     gene            1469..2332
                     /gene="purU"
                     /locus_tag="PKN79_01390"
     CDS             1469..2332
                     /gene="purU"
                     /locus_tag="PKN79_01390"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNZ94446.1"
                     /translation="MNNKIVFLIQSEDRKGILSATTTFFSTLGFNILHCQQHTDAREG
                     RYFMRIELDLSDLPSTRSDLEKRFARFAEEQRLTWRCHWGDYRSRVAILVSKTSHCLY
                     DLLFHVSEGDLFCDIPLIISNHPDLESVANLFRIPYYHLPVTKDTKEEQEAEILRLLE
                     RFDIDLVVLARYMQILTPSFTDRWYGRIINIHHAFLPAFQGANPYQQAYERGVKMIGA
                     TAHYASEELDRGPIIEQDVVRVSHELDPSGLRNLGKDVERRVLTKAVSAHLESRVIIY
                     KNRTIVFGPIV"
     gene            complement(2329..3432)
                     /locus_tag="PKN79_01395"
     CDS             complement(2329..3432)
                     /locus_tag="PKN79_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNZ94447.1"
                     /translation="MADQRQIRLAKLLVEYSTKVQKGEKVLINAVDVPVPMVEELIKA
                     VYEAGGFPIVNLWNQRIERAIVEGASEASLKAWADVDMYRMNRMDVFIGIRGVANVRE
                     LATIPNQNNLASLHYSKPVHMDIRVPKTRWVVLRYPTEVMALQANMGTREFEDFFYSV
                     CTDVDYAKMAKAMAEAEKYLDTVDKVRIVAPGTDLRFSVKNIPWVPCAGEMNIPDGEI
                     YTAPVKDSVNGTITYNTPSTNGGHLFRDIRFTFKDGKIIEAHADDDRLLNEILDRDEG
                     ARYVGEFALGCNPQILHPMDNTLFDEKISGSFHFTPGNAYEDADNGNRSSVHWDLIQI
                     QTPEFGGGEIYFDGVLVRKDGVFVDERLAMLNL"
     gene            complement(3439..>4101)
                     /locus_tag="PKN79_01400"
     CDS             complement(3439..>4101)
                     /locus_tag="PKN79_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNZ94448.1"
                     /translation="VDKPAGLATVPLKEDPPDKPTLLGVVASEYPETLKPFGRNTWEG
                     GVLHRLDTPTSGLVIIARTQYGFDALTAASRAELVTKEYWLGSHLRAKPPKGFPAYPY
                     EDPAFAGGKEVVIGSLFRPWGEKRREVRPVLSESPRHLLEKTSGVWYMTKVVAQGSGV
                     FRARITSGFRHQVRAHLAWSGHPIDGDDRYGGKPDEKLLLRATAVEFPFDGRIREVRV
                     KE"
BASE COUNT          911 a         1165 c         1111 g          914 t
ORIGIN      
        1 ttcccgagta aggagtattg ctttgcgatt gagacagctt atcggggctg tctctttttt
       61 ttcaaaggat tgttgacgtg gatgttccca ccattacggt taccgaattg acccgctcga
      121 ttaaacaggt tcttgaggaa ggattcccct tcttttcggt caccggagaa atctcaaact
      181 ttcgccccgc ttccagcggt cactggttct ttaccctcaa ggacgaggga gctcagatcg
      241 gttgcgtaat ctttcgctca aacgcgtgga aagtaacctt cacccctcaa gacggggaca
      301 aggtgacgat tacaggatcg ttggatctct atcctcctcg aggaacctat cagatcaagt
      361 gcgagacgat cgtaaaaacc ggcttcggaa acctgctcgc tctcctcgaa gagcggaaaa
      421 cgcgctacca gcaaaaaggg tattttgacc gcaagcgccc gttgccgaaa actccgagaa
      481 agctgggcat catcacaagc gccaccggag cggcgttgca ggacgtgctc tcggtccttg
      541 ggaggcgagc cccctccttg gagatcttga tcttcccttc cccggttcaa ggcgccgaag
      601 cggctcaatc aatcgcattt cggatcgaac aggcgaatct gctttccgac tgcgacgtgc
      661 tcctgatcac ccgcggcggc ggctcaatcg aagatctttt gccgttcagt gaagaggcgg
      721 ttctggaagc gattcatgca tccgcgattc ccaccgtcag tgcaatcggc catgagattg
      781 actgggccct cagcgacttc gtcgccgacc tgcgcgctcc gactccgtct gcagcggccg
      841 agttgatcag caagggaatc tacgattcca agaccgagct tgttcgaatc aagcaacgcc
      901 tgatcgccgc aatcgaatac cggctcctgc gggcggaagc ggcggttggg aaagtcagcg
      961 ttcctgcact gagggcatat ctgcttgctc aggtcgaccg caggcaattc cgtttggcaa
     1021 atgtccagct ggccctttcc aacggtattg acgatcagct tgcccgaaga agaaacaaac
     1081 tgacgctgct gtccaatcaa ttaagcgcct taagtccgct tgccgtcctt gagcggggat
     1141 tcgcccttgt ctctacaaaa gagggggcgg ttcttcgaag cagcaagccg gttcacgatg
     1201 gtgacaggct gaagattcgt tggatcgatg gcgaacgatc ggtccgagtg gaggaacagg
     1261 atgaactttg aagaaaagat tgcaaaaatc gaaacgatta ccaaaaaact gcaggatgag
     1321 cacacctcgc tcgaagattc aatcgctctc tttgaagaag gggtaacact ggcaaaagaa
     1381 cttgagcaag cgttggaaga agcaaaagga aaagtggaac aagtggtcgg tgaaagcttg
     1441 gcctcgatgg aagtggtgga attcgacgat gaacaataag atcgtctttc tgatccaaag
     1501 cgaggatcgg aaaggaatcc tcagcgcgac cacgaccttc ttcagcaccc tgggcttcaa
     1561 catcctccac tgccagcagc ataccgatgc ccgggaaggg cgctacttca tgcgcatcga
     1621 gctggatctc tccgatctgc cgtcgacgag gagcgatctg gaaaaacggt tcgcccgctt
     1681 tgccgaggaa caacggctca cctggaggtg ccactggggc gactaccgga gccgggtcgc
     1741 gattctcgtc agcaagacca gtcactgcct gtatgacctg ctcttccatg tcagcgaggg
     1801 ggatctcttc tgcgacatcc ctttgatcat cagcaatcac cccgatctgg aaagcgtggc
     1861 gaacctgttt cggatcccct attaccatct gcccgtgacg aaggatacca aggaggagca
     1921 ggaggcggag atccttcgac ttctggagcg cttcgacatc gatctggtcg ttctggcccg
     1981 ctacatgcag atcctcaccc cctccttcac cgatcggtgg tatggaagga tcatcaacat
     2041 ccaccatgcg ttcctccccg cattccaagg ggcgaacccc tatcagcagg cgtatgaacg
     2101 gggggtgaag atgatcgggg cgaccgccca ctatgcgagc gaggagctcg accgggggcc
     2161 gatcatcgaa caggacgtgg ttagggtcag ccacgagctc gacccttcag gcttgaggaa
     2221 cctcggcaag gatgtggaac ggcgcgtgct caccaaggcg gtgagcgcgc atctcgaaag
     2281 ccgggtcatc atctacaaga atcggacgat cgtcttcggc ccgatcgtct aaagattcaa
     2341 catcgcaagg cgctcatcga cgaaaacccc gtccttgcgg acaagaactc cgtcgaagta
     2401 gatctctcca cccccaaact cgggggtctg gatctgaatc aggtcccagt gcaccgaaga
     2461 gcggttgccg ttgtcggcat cctcgtaggc gttgcccggg gtgaagtgga acgatccgga
     2521 aatcttttca tcaaaaagcg tgttgtccat cggatggagg atctgcgggt tgcagccgag
     2581 ggcgaactcc ccgacgtagc gcgctccttc atcccggtca agaatctcat taagcaggcg
     2641 atcgtcatcg gcgtgcgctt cgatgatctt tccatccttg aaggtaaatc ggatatccct
     2701 gaagagatgt ccgccattgg tcgacggtgt gttataggtg attgttccgt tgaccgagtc
     2761 tttgaccggg gcggtgtaga tctccccgtc gggaatgttc atttcgcctg cgcacggcac
     2821 ccaaggaata ttttttacgc tgaagcgcaa gtccgtgccc ggagctacga tcctaacctt
     2881 gtcgacggta tcgaggtact tctccgcctc cgccatcgct ttcgccatct ttgcatagtc
     2941 gacgtcggtg catacgctat agaaaaaatc ctcaaactcc cgcgttccca tgttcgcctg
     3001 aagagccatg acttcggtgg gatagcggag tacgacccac cgggtcttgg gaacccggat
     3061 atccatgtgg accggtttgg agtaatggag cgaagcaaga ttgttctggt tggggatcgt
     3121 ggcaagttcg cggacattgg cgacgccgcg aattccgatg aacacgtcca tccggttcat
     3181 ccgatacata tcgacatccg cccatgcctt gagcgacgct tcgcttgcgc cttcgacgat
     3241 cgcccgttcg atcctctggt tccagaggtt gacgatcgga aacccgcccg cttcatatac
     3301 cgccttgatc agctcttcta ccatcggaac gggaacatcc accgcgttga tcaaaacctt
     3361 ctcgcctttt tgcactttgg tcgaatactc gaccaacaac ttggctaatc ggatctgtcg
     3421 ttgatctgcc atctcttctt actcctttac tcgtacttct cttattcttc catcaaaggg
     3481 gaattcaacc gcggtcgctc gaagaagaag cttctcatcc ggtttgcccc cgtatcgatc
     3541 atcaccgtcg atcggatgtc cgctccacgc aagatgtgcc cgcacctggt ggcggaagcc
     3601 gctggttatc cgggctcgaa acactcccga accttgagca acgactttcg tcatgtacca
     3661 cactccgctg gttttctcca agaggtggcg cggcgattcg ctgagaaccg ggcgcacttc
     3721 ccgtcttttt tctccccaag gacgaaacag cgaaccgatg acgacctctt ttcctccggc
     3781 aaacgccgga tcctcatagg gataggcagg aaagcctttc ggcggtttgg cccttagatg
     3841 tgaaccgagc caatattctt tcgtaaccaa ttcggctcgg gaagcggctg tgagggcgtc
     3901 aaacccatat tgggtccggg cgatgatgac cagcccgctg gtgggtgtat ccaatcgatg
     3961 gagcactcct ccttcccaag tgtttcggcc gaacggcttg agagtttcag gatactctga
     4021 ggcgacaacc ccaaggaggg tcggtttgtc gggaggatct tccttcaaag gtacggtggc
     4081 caaacccgcc ggcttgtcca c
//
LOCUS       DAOPUZ010000043         4085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_22091_length_4085_cov_3.339454, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000043 DAOPUZ010000000
VERSION     DAOPUZ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22091_length_4085_cov_3.339454"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..831
                     /locus_tag="PKN79_01405"
     CDS             52..831
                     /locus_tag="PKN79_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNZ94449.1"
                     /translation="MRYGNELREKLKNGRAIGGHVFLGDPAISEALAIYGYDFIWIDA
                     EHGAFDRPLILSHVIAANAGGAAAVVRVPSWSMEAVKPVLEMGIDGIIIPQTKGYEDA
                     KAALAECLYPPRGVRGFGPRRAIRYNDLDTGEYLRNAEDEIVRIIQIEHHKAVEEIDR
                     TLTLDGLDALVLGPNDLASSIGLIGEALHPKVLDLSQHAIAQAKKAGVPIGVSIGPNQ
                     EAIDMWRGLGVDYFSCGDDISFLHQGAKATIEAIQKGQRRE"
     gene            824..1567
                     /locus_tag="PKN79_01410"
     CDS             824..1567
                     /locus_tag="PKN79_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNZ94450.1"
                     /translation="MNEEGSVLDIEHITLSRTNLSMQIADHLEEVILTSPSKVSDKLP
                     SEMNLAKQYNVSRPVVREAIKLLQERGLVTLKTGSGAYITRPETSTVMNAINRIMQVD
                     QISSEELTEVRAILELSGIELAVGKITDEELEEIDSILTQFENKTLPFKVRVSLDEAF
                     HLALAKATGNNLLALFIGTLTSLLRDYMGKGILIEGGIEDAIARHRQIYHALRARDKE
                     AAIQAMREHLKVSSYNVRVFDEQLHKKKK"
     gene            complement(1549..2688)
                     /locus_tag="PKN79_01415"
     CDS             complement(1549..2688)
                     /locus_tag="PKN79_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNZ94451.1"
                     /translation="MELLGSLSRYISQQLDSRKVDSIKKEEFRKTLAAALLMPAFEKP
                     TVTSLWVTEHDALRIEKLMWNVGFGCSEGYLLSPSGAKEKLPGILALHDHAGEKYWGK
                     SKITDIGEKLDRSLVEHQKRYYGARAWANELAKRGYAVFVPDLFGFESRRIKAAELPQ
                     SLIQNLLTPPLEIVEGGAYWNDDLVLDDNLDSRVAYNALARGLEHVIAKVLMSGGYTW
                     AGYTVAEDLVALDILSEHELVDSSRLGCGGLSLGGLRSVLLSSLSPKIQASFVTGFMT
                     TFKDLVNAKAANHTWIAYAPRLSHLGEFGDLLLAYAPKPLLVQHGKRDSLFTFSEVER
                     SALRIREHYKDDSEQFVFTVYDEPHLFNEPMQEEAFAFFDHFFFL"
     gene            2825..3892
                     /locus_tag="PKN79_01420"
     CDS             2825..3892
                     /locus_tag="PKN79_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNZ94452.1"
                     /translation="MKLLDIFKKLVAIDSPSFEEDRVALFVEQKLLASGFSTRYDEAG
                     NLYGYLDGEGDALLLNTHMDTVDLAVGARVVVEDGIIRSDGTTALGADDKAAVAAVLY
                     ALEKVVSAGRKHPPLVVLFTTAEEVGLAGAKQVDRSLLDGVAFGYTFDASGPVGGAII
                     QASWHDRLDVVIGGKAAHAGFKPEDGVSAIKIGSDAIASMRLFRIDEETTANVGSFVA
                     PGATNVINPEATMSFEARSLDEKKIDKQIAHMIEVLEKKAESAGGTVHTHHHRLYEGY
                     RHQADTPVVKRFIEACEALKIPVELKKTQGGSDANIFNALGIPTLTCSVGYENAHSKE
                     EYIPLVELERLASLIKEMIVR"
     gene            3889..>4085
                     /locus_tag="PKN79_01425"
     CDS             3889..>4085
                     /locus_tag="PKN79_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNZ94453.1"
                     /translation="MKNVVIVGAGAVGALYGQRLNSVCSLSFLVDEKRKERYLRDGII
                     INGVLAPFTYACADEIGVPDLV"
BASE COUNT          924 a         1164 c         1152 g          845 t
ORIGIN      
        1 cagcctgatc cggcttgaag gccaattgag ggcccgtttg ggattggcgc catgagatac
       61 ggaaacgagc tgcgtgaaaa actgaagaac ggaagggcga tcggtggaca cgtcttctta
      121 ggggatcctg cgatcagcga agcgcttgcg atctacggct atgatttcat ctggatcgat
      181 gccgagcacg gagcgttcga ccgacctctc attctgagcc acgtcatcgc cgcaaacgcc
      241 ggaggagccg ccgcggtggt gcgggttccc tcttggtcga tggaagcggt taaaccggtt
      301 ctggagatgg gaatcgacgg catcatcatt ccccagacca aggggtatga agatgcaaaa
      361 gcggcgctgg ccgagtgcct ctaccctcct cggggggttc gaggcttcgg cccgcgaagg
      421 gcgatccgct acaacgacct cgacacgggt gaatacctca ggaacgcgga ggatgagatc
      481 gtccggatca tccagatcga gcatcacaag gcggtcgagg agatcgaccg gactctcacc
      541 ctcgacgggc ttgatgcgct cgttctgggt ccgaatgacc tcgcctcctc gatcggtctg
      601 atcggcgagg cgctccaccc aaaggtcctt gacctgtcac agcatgcgat cgctcaagcg
      661 aagaaagcgg gtgtccccat cggcgtctca atcggcccca atcaggaggc tatcgacatg
      721 tggagaggat tgggggtaga ctacttcagc tgcggagatg atatctcctt cctccatcag
      781 ggggcgaaag cgacgatcga agcgattcaa aaaggacaac gccgtgaatg aagaaggttc
      841 tgtactcgac attgagcaca ttaccctttc aagaacaaac ctcagcatgc agatcgccga
      901 ccaccttgag gaggtgattc tcacctcccc gtcgaaggtc agcgacaagc tcccaagcga
      961 gatgaatctc gccaagcagt acaatgtcag ccggccggtt gttcgggagg cgatcaagct
     1021 gctgcaggaa cgcggcctgg tcaccctgaa aaccggaagc ggggcgtaca tcacccgccc
     1081 tgaaacctcc acggtgatga atgcgatcaa ccgcatcatg caggtcgatc agatcagcag
     1141 cgaggaattg acagaggtcc gggcgattct tgagctttcg ggaatcgagc tcgccgtcgg
     1201 gaagatcacc gatgaggagc ttgaggagat cgattcaatc ctcacccaat tcgaaaacaa
     1261 gaccctcccc ttcaaggtcc gggtcagtct ggacgaggcg ttccacctcg ctcttgcaaa
     1321 agcgacggga aacaatctct tggccctttt tatcggaacc cttacgtccc ttcttcgcga
     1381 ttatatgggc aagggaatcc tcatcgaagg gggaatcgag gatgcgatcg cccgccaccg
     1441 gcagatctac catgccctcc gcgcccggga caaggaagcg gccattcagg cgatgcgcga
     1501 acacctgaaa gtctcctcgt acaacgtccg ggtcttcgat gaacagcttc acaagaagaa
     1561 aaaatgatcg aaaaacgcaa acgcttcttc ctgcatcggc tcattgaaga gatgcggttc
     1621 gtcatagacg gtaaagacga attgttccga atcatccttg tagtgctctc tgatccgcaa
     1681 agcgcttcgc tcgacttcac tgaaggtgaa gagcgaatcc cgttttccgt gttgaaccag
     1741 aaggggcttc ggagcgtaag ccaataacag gtccccgaac tctccgaggt gactgagccg
     1801 gggagcatag gcgatccagg tatgattcgc cgctttggcg ttcaccagat ccttgaaggt
     1861 ggtcatgaac cccgtcacga agctcgcttg gatcttcgga gagagcgaac tgagcagcac
     1921 gctcctcaac cctccgaggc tcaagccccc gcaaccgagg cgggaactgt cgacaagctc
     1981 gtgttcggag aggatatcca aagcgaccag atcttcggcg accgtgtatc ccgcccacgt
     2041 gtagccccca ctcatcagca ctttcgcaat gacatgctcc agcccccggg caagggcgtt
     2101 atacgcgacc ctgctgtcaa ggttgtcatc caagacaagg tcatcgttcc aataggctcc
     2161 tccttcgacg atctccagcg gaggagtcaa caggttttgg atgagtgatt ggggcaattc
     2221 cgcggctttg atccttcggc tttcaaatcc gaagagatcg ggaacaaaga ccgcatagcc
     2281 ccgtttggcc aattcgttgg cccaggctcg tgcgccatag taccgttttt gatgttcaac
     2341 caaagatcga tcgagcttct ctccgatatc cgtgatcttg ctctttcccc agtacttctc
     2401 cccggcgtgg tcatggaggg caaggatgcc gggcagcttt tccttggcac ccgacgggga
     2461 gagcagatac ccttcactgc agccgaatcc gacgttccac atcaattttt cgatccgaag
     2521 cgcatcatgt tccgttaccc acaggcttgt cacggtcggc ttttcaaagg cgggcatcag
     2581 caatgccgct gccagtgttt ttcgaaactc ctctttcttg atactatcaa cctttcggct
     2641 gtcgagctgt tgactgatat atcgactcaa cgatccaagc aactccatca aacaacctcc
     2701 aatcacatgc aaacaccggt tttcatctct agaatagcac acaccatcag cgtgtgacat
     2761 ggaaacctcc tccttcgccc tcgcctttct tctctgtctc cccgctttat gctaagctgg
     2821 ccccatgaaa ttgcttgata tcttcaaaaa gcttgtcgcc atcgattcgc cctccttcga
     2881 ggaggatcgg gtcgcgttgt tcgtggagca gaagcttctg gcgtcggggt tcagcacccg
     2941 ctatgacgaa gccgggaatc tctacgggta ccttgatggc gagggagacg ccctcctgct
     3001 caacacccac atggatacgg tggacttggc cgtcggggct cgcgtggtcg ttgaagacgg
     3061 catcatccga agcgatggaa ccaccgccct cggggcggat gacaaggccg ccgtggcggc
     3121 ggtgctgtat gcccttgaaa aggtcgtctc ggcgggcagg aagcacccgc cgctggtggt
     3181 gctcttcaca accgccgagg aggtggggct tgccggggcg aagcaggtcg atcgttcact
     3241 gttggacggt gttgccttcg gatatacgtt cgatgcctcc ggcccggtgg gcggggcgat
     3301 catccaagcc tcctggcacg accggctgga tgtggtcatc ggcgggaaag ccgcccatgc
     3361 cggcttcaag ccggaagatg gagtcagcgc gatcaagatc ggttcagatg cgatcgcttc
     3421 gatgcgcctc ttccgcatcg atgaggagac cacggcgaat gtgggctcct tcgtcgcacc
     3481 cggggccacc aatgtgatca acccggaagc gacgatgagc tttgaagcaa gaagcctaga
     3541 tgaaaaaaag atcgacaaac agatcgccca tatgatcgag gttctggaaa agaaggccga
     3601 atcggcgggg ggaacggttc acacccacca ccatcgcctc tatgaagggt accggcatca
     3661 agccgacact ccggtcgtca agcgcttcat tgaagcgtgt gaagcattga agattccggt
     3721 ggaactgaag aagacgcaag ggggcagcga tgccaatatc ttcaatgcct tggggattcc
     3781 cacccttacc tgctctgtcg gctatgagaa cgcccactcc aaagaggaat atattccgct
     3841 cgttgagctg gaacgcctgg catccctgat caaggagatg attgtcagat gaagaacgta
     3901 gtcattgtcg gagcgggggc ggttggcgcc ctctacggtc agcgcctgaa ctcggtgtgc
     3961 tcgctgtcct ttctggtgga tgagaagcga aaagagcgct accttcggga tggaatcatc
     4021 ataaacggcg tactcgcccc gtttacatat gcatgtgccg atgagatcgg cgtgcccgat
     4081 cttgt
//
LOCUS       DAOPUZ010000044         4084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_22099_length_4084_cov_3.409034, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000044 DAOPUZ010000000
VERSION     DAOPUZ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22099_length_4084_cov_3.409034"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKN79_01430"
     CDS             <1..856
                     /locus_tag="PKN79_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ94454.1"
                     /translation="DETVKALLQGLGHVESKVNKPLLPGYGIGSPGLIDPKSGTVFRA
                     DDMGWTKPLPLKQLVEEATGKKAYIINRYRANGVAELTFGGHDLTKNIIYLGVGTGIA
                     GSVYLEGTLLNSTKYRFGHMVIDPHGPLCGCGQYGCLQAMASEKALLTYARERMKEDL
                     TFLAAYADQPITAKLLVDLAEGGDPHAQSCLSHIASALGIAILTLANVIAPDEIVIGG
                     PIGDASTFLVDEVRKAAYAHLLDWQVSSLKISKGSQGDYGSVLGAAAFLLEQKMELLL
                     GEGCPVIS"
     gene            complement(853..1902)
                     /locus_tag="PKN79_01435"
     CDS             complement(853..1902)
                     /locus_tag="PKN79_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ94455.1"
                     /translation="MKKLGFGIIGLGAIADFHARAIKELDGCELVNCYHYREERANEF
                     AKKHGCTAYTDLDKFLADDRLDIVAIATPSGAHLDGALAAAKAKKHVIIEKPLEITKD
                     RCTQIINACKEHNVKLAGIFQSRFFDSSKILKEAIEKGRFGKIVMADAQVKWFRSQAY
                     YDSAAWRGTWALDGGGALMNQSIHAIDLLLWLVGDVSEVFAFADTLSHERIEVEDTAV
                     AVLRFNSGAMGVIEGTTSAYPGFLKRLEILGTKGSAILEEESIIAWQFEDEEPGDEEI
                     RKTFLDSTETGGGAADPLAIGHHGHTRNFASFVKSIREDSPVEVSGFEARRSVELIEA
                     IYRSAKSGKKIELPL"
     gene            2056..2958
                     /locus_tag="PKN79_01440"
     CDS             2056..2958
                     /locus_tag="PKN79_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94456.1"
                     /translation="MDLFPALLAIIISGFAGGIFSFGYKAIVQLKQRIGPFIFLYGLT
                     YTIFAAVGILLIGGVSADPIVWLFGVSHGVIMCMAILFYIRIIGKIRLSISWTIIQFS
                     IFIPLVTSLLFFEKHLSPLSVGGTVLMLVAIIIFSFGKKEHSSKPEGPPITLASWLLL
                     FLSSLFSGMALTIPMVYMKAATTSQPLYLIFASGVGTLLCSLLFIRKEDWKYMKGDWK
                     VVGVPSAMSLLQIMSWAFLMVGLLDLDGAVAYSLKGFIGLLCVYTVSFSFFRETVTRI
                     EKIGILVSLGAILLMTVSLYTVAL"
     gene            complement(2972..3802)
                     /locus_tag="PKN79_01445"
     CDS             complement(2972..3802)
                     /locus_tag="PKN79_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94457.1"
                     /translation="MKIIEQLCMGKSPDLENEDGLFINEHFAAVIDGCSSRKPLSGQT
                     RTNGVIAKNLLLACLENLAPHASKDETFHALNDSIRRWYGTRDLLEAAEEDAELRPGA
                     YIAMLSNARREIWILGDCQALADGVHYTETKAIDTLMELNRTMLIEEALIQGFSREHL
                     LRHPELIQNRLSEFMTHQASFQNRMVGTSTFGYPTLDGFFDHYESIIVVQLGSDSKEV
                     VLASDGYPFLRPTLEESEMKLKQLLRKDPLLYTEYRATKGLNPAYTSFDDRSYLRIRI
                     "
     gene            complement(3799..>4084)
                     /locus_tag="PKN79_01450"
     CDS             complement(3799..>4084)
                     /locus_tag="PKN79_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94458.1"
                     /translation="DVGHLWIAGQVYGFDYLEGIKEILATKKVITAHVHSNPLDRGRG
                     IFVDSHDSIDQHSFDFRTVLKLLADSGANIILETVNSPKRNTLLLYEALG"
BASE COUNT          906 a         1110 c         1027 g         1041 t
ORIGIN      
        1 ggatgaaacg gtcaaagcgc tccttcaggg attggggcat gttgaatcaa aagtgaacaa
       61 accgctgctg ccgggctatg gaatcgggtc gccgggcttg atcgacccca aaagcggaac
      121 cgtcttccgg gccgacgaca tgggttggac aaaaccactt ccactcaagc agctggttga
      181 agaagcgacg gggaagaagg cgtacatcat caatcgctat cgggcgaacg gggttgccga
      241 gctgaccttc ggcggacatg atttgacgaa aaacatcatt tatcttgggg tgggaaccgg
      301 cattgccggc tccgtatacc ttgaaggaac gcttctgaac tccacgaagt atcgcttcgg
      361 gcacatggtg atcgatcccc acggaccgct gtgcgggtgc ggccagtacg gctgccttca
      421 agcgatggca tcggaaaagg cgctcctcac atacgcgcgt gagaggatga aagaggattt
      481 gacattcctg gcagcgtacg ccgaccaacc aataacggca aagcttctcg tcgatctcgc
      541 cgaagggggg gatcctcacg cacaatcgtg tctatcgcac atcgcatcgg cgcttgggat
      601 cgccattctg acgcttgcca atgtaatagc ccccgatgaa atcgtcatcg gaggcccgat
      661 cggggatgcc agcaccttcc ttgtggatga ggttcgcaaa gcggcgtacg cgcacttgct
      721 tgactggcaa gtctcgtcac tgaaaatcag caagggaagc caaggtgatt atggctccgt
      781 gttgggagct gcagccttcc tcctggagca aaaaatggag ttgctcctcg gcgaaggctg
      841 cccggtgatc tcttaaagcg gaagctcgat tttctttccg ctcttggcgc tccgatagat
      901 cgcttcaatc aattcaaccg atcttcgagc ttcaaaaccg gagacttcga cgggagaatc
      961 ctctctgatc gatttcacaa aggaagcaaa attccttgta tgaccatggt gaccgatcgc
     1021 cagcggatcg gcggctcctc cgccggtttc cgttgaatca aggaacgttt tgcgaatctc
     1081 ttcatcaccc ggctcctcat cttcaaactg ccaggcgatg atcgactctt cctccagaat
     1141 cgcacttccc ttcgtgccaa ggatctccaa acgtttcaag aatccgggat aggcgctggt
     1201 tgttccttcg atgactccca ttgcaccact gttgaatcgt aacaccgcca ctgcggtatc
     1261 ttcaacttcg attcgctcgt gggaaagcgt atcggcaaag gcgaatactt ccgaaacatc
     1321 accgaccagc cacaagagca agtcgattgc gtggatcgat tggttcatca acgcaccacc
     1381 gccgtccaac gcccacgttc cccgccatgc tgcactgtca tagtacgcct ggcttcggaa
     1441 ccattttacc tgggcgtccg ccatcacgat ctttccgaaa cgccccttct cgatcgcttc
     1501 tttaaggatt tttgaagaat caaagaaccg ggactggaaa ataccggcaa gctttacatt
     1561 gtgttccttg catgcgttga tgatctgagt acagcgatcc ttcgtgatct ccagcggctt
     1621 ctcaatgatt acgtgcttct tcgctttggc tgcagccaga gccccgtcaa ggtgggctcc
     1681 cgacggagtg gcgatcgcaa cgatgtccag ccggtcatcc gcaaggaact tgtccagatc
     1741 ggtatatgcc gtgcatccat gcttcttcgc aaactcattc gcccgttctt cccgatagtg
     1801 gtaacagttt acgagttcac agccgtccaa ctcttttatc gcccgagcat gaaaatcggc
     1861 gattgcaccc aatccaatga ttccaaatcc aagttttttc atcgctacct ccgatatgtg
     1921 tacctacacg ctaccatcaa gggaatggaa agtcaaattt ttttgtaata tcgtattaca
     1981 aattattttg gacggttaca ttgttcgggt attgccgtat actgaccttg accatctctt
     2041 cgacttggag ctcacatgga tctgtttccg gcccttttgg caatcatcat ctccggcttt
     2101 gccggcggca ttttttcttt cggatacaaa gcgatcgttc agctgaaaca acgaatcggt
     2161 cctttcatct ttctctatgg gctgacctat acgatattcg cagctgtcgg cattctgttg
     2221 atcggtggag tttccgccga tcccatcgtg tggctcttcg gggtttcgca cggtgtcatc
     2281 atgtgtatgg cgatcctatt ctatatccgc atcatcggaa agattcgcct cagcatctcc
     2341 tggacgatca ttcaattctc aatcttcatt ccgctggtca ccagccttct cttctttgag
     2401 aaacatctta gtccgctctc ggtcggcgga accgtgttga tgctggtagc gatcatcata
     2461 ttcagttttg ggaaaaaaga gcactcgtcc aagcctgagg ggcctccgat cactcttgca
     2521 agctggctgc tcctcttctt gtcttcgctc ttttcgggaa tggcgcttac cattcccatg
     2581 gtgtacatga aagcggccac cacaagtcag cccctctacc tgatcttcgc cagcggcgtc
     2641 ggcaccctcc tgtgttccct gttgttcatc cgaaaagagg attggaagta catgaaaggg
     2701 gattggaaag tggtcggcgt cccttcggcg atgagccttc tgcaaataat gagctgggcg
     2761 tttctcatgg tcggcctgct ggaccttgat ggcgccgtcg cctactccct caagggtttc
     2821 atcgggctgc tctgcgtcta caccgtttca ttttcgttct tcagggaaac cgtcacccga
     2881 attgaaaaga tcggaatcct cgtctccttg ggagcgattc ttcttatgac ggtctcgctc
     2941 tacaccgtcg ctctgtagat taacgaatca atcagatacg aatccggaga tagctcctgt
     3001 catcaaacga ggtatatgcg ggattcaacc ccttggtggc tcgatattcc gtatacaaga
     3061 gaggatcctt ccggagtagt tgcttgagct tcatctcact ctcttcgagg gtcgggcgca
     3121 ggaagggata gccgtcactg gcaagaacga cttctttgga gtcgctaccc aattggacga
     3181 cgatgatcga ttcatagtgg tcgaagaatc cgtcaagagt gggatacccg aatgtcgatg
     3241 tgccgaccat ccgattctga aaggatgcct gatgagtcat gaactccgac aatcggtttt
     3301 gaatgagctc aggatgacgc aggaggtgtt cgcgtgagaa accctgaatc agcgcctctt
     3361 caatgagcat tgtccggttc agctccatca acgtgtcgat cgcttttgtt tcggtgtagt
     3421 ggactccgtc agcaagtgcc tggcagtcac cgagaatcca tatttcccga cgagcgttgc
     3481 tcagcatggc gatataggcg ccggggcgca attccgcatc ttcttcggct gcttcaagaa
     3541 gatcgcgggt tccataccac cggcgaatcg aatcattgag cgcatgaaac gtctcatcct
     3601 tcgaggcgtg cggggcaaga ttctccaaac acgccagcag aaggtttttc gctatgacgc
     3661 cattcgttcg ggtttgtccc gaaagaggtt ttcttgagga gcatccatcg atgaccgctg
     3721 caaaatgctc gttgatgaag agcccgtcct cgttttcaag atccggtgac ttgcccatgc
     3781 acagctgctc gatgattttc atcctagtgc ctcgtacagt aacagcgtgt ttcgcttcgg
     3841 agagttcacg gtttccaaga tgatgttcgc cccgctgtca gcaagcagtt tcaacacggt
     3901 acgaaagtcg aaggagtgtt ggtcgatgct gtcatgcgag tcgacgaaga tacctcgtcc
     3961 tcgatctaaa gggtttgaat ggacgtgggc ggttatgact ttcttcgttg caagaatctc
     4021 tttgattcct tccagataat caaacccata gacttgaccg gcgatccaca aatggccgac
     4081 gtca
//
LOCUS       DAOPUZ010000045         4063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_22252_length_4063_cov_3.909681, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000045 DAOPUZ010000000
VERSION     DAOPUZ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22252_length_4063_cov_3.909681"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PKN79_01455"
     CDS             <1..594
                     /locus_tag="PKN79_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNZ94459.1"
                     /translation="TADSYTLRARKEGYPARSVYKLEEIQKQFAIIKKGDTVLDVGAA
                     PGSWTLYTGNVLLKGVGKIVAVDLNPLNINPIPHTVISYVGDAFGKEIKPKLIEHGPY
                     DAIISDAAPLTTGNRGVDTARSEYLAEQVILLAAEHLKAGGNLVVKLFQGGGEQELMK
                     RLRTLFTRAKIVKPKACRDDSFEIYLVGVGRKEGEQG"
     gene            591..1010
                     /locus_tag="PKN79_01460"
     CDS             591..1010
                     /locus_tag="PKN79_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HNZ94460.1"
                     /translation="MKITVIDGQGGSLGKALVAAIKKRFPHEEVFAIGTNALAASAML
                     GSGADAAASGENPVLVACRKSEVIIGPIGLLAADSLHGEITPAMAVAIGQSSAHKILL
                     PITKCNVSIVGKQELSTKEMIEGAVAELASYLVDRSL"
     gene            1118..2149
                     /locus_tag="PKN79_01465"
     CDS             1118..2149
                     /locus_tag="PKN79_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HNZ94461.1"
                     /translation="MTRYQQPLEEGLSLAVYNGDDLIFTHQGHWLHPLFALEEFLITY
                     EGPRNLLSVHDSAAGKAAAVLQARLGVRRAHIDLISDLAVEYYRAKGIEVSWEKKIER
                     LACQTESLLSEIDDEDEIYRILKRRAKLVQGVGVEVAGISFAYPNSEPLFENLHFTLP
                     EGGRLIIQGDNGMGKTTLINLLLGRLLPTAGAIRIDGREPGELPPRTIGYIKQQQTQE
                     QFPVSAREVVAMAVDPSLPRERQAWEIDTALRRTASAHLSSRNFFSLSGGERQRVSLA
                     RTLCQKARLLLLDEPTSFLDAKSRTTLVGLLHSLTLAEMPTIIIVTHDKELEEDLGWE
                     ILRLGGDHE"
     gene            2142..2948
                     /locus_tag="PKN79_01470"
     CDS             2142..2948
                     /locus_tag="PKN79_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNZ94462.1"
                     /translation="MNDFLFALTLAPVARGLLAMAIAGLAFPATGVMVLRLNLIPMRY
                     MLMHGVILAGAISLALNVNTLAISVVLNLILVLLMLRLTKDTRFGFGLSSATAMVFTM
                     AAASLVMHIWDVPAKDTLQLLWGSPFALTWTDISALIALAIIIVLYMFMSFRTISALF
                     FDQEIASSLGMRVRFHHTFMVTLIALVIALAMKLLGALLIDALLILPVLVASKRSESL
                     KRLVLSACIIGFFVSTLGYLLAIYTDMPPSGTIALLSALIYPIPTFKKRS"
     gene            2951..3703
                     /locus_tag="PKN79_01475"
     CDS             2951..3703
                     /locus_tag="PKN79_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ94463.1"
                     /translation="MKRIVVLILAALLSFTLFAQAVPEKQESTVVASTAWTAAFADIA
                     GVDNVDFIAPATMTHPPEYEITVGDVQKINNAEYFLYAGYEVMMKSMGTTIKKDESAL
                     IHIQTNNSIENVRTQSAKLAAIFGTETENKVRFATYEEGILDGKKQVAAKGLDKKNVY
                     CHSMQVYLAKDLGLNVVGTFGPAPLTAAQLAEIAKGEIDIIIDNIHNPVAPPALEVSP
                     KSRIVTWRNLPGRGGRGTLEEMVRSNIAELLK"
     gene            complement(3747..>4063)
                     /locus_tag="PKN79_01480"
     CDS             complement(3747..>4063)
                     /locus_tag="PKN79_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNZ94464.1"
                     /translation="FGRAIKQQLQVIGPIFGSTDAAPVSVAEAMIAFAKSIHAPTKLS
                     DVEGFTEAHITRALAAAKDPQLKMKLQNMPIPMDASDVDTYMAPLLRAAAEGDLTRIK
                     AM"
BASE COUNT          878 a         1195 c         1154 g          836 t
ORIGIN      
        1 actgccgatt cctacaccct tcgggcccgc aaagaaggct atcccgcccg cagcgtgtac
       61 aagctggagg agatccagaa gcagtttgcg atcataaaaa agggcgatac ggtcttggac
      121 gtcggggcgg ccccgggctc gtggaccctc tataccggca acgtcctgct caaaggggtg
      181 ggaaagatcg tcgcggtgga tttgaatccg ttgaacatca acccgatccc ccataccgtc
      241 atctcctatg tcggtgatgc gttcggaaag gagatcaagc cgaaactcat cgaacacggg
      301 ccgtatgatg cgatcatcag cgatgccgct ccgctgacga cgggaaaccg cggcgtcgat
      361 accgcccgta gcgaatacct tgctgaacag gtgatcctcc tcgcagccga acacctcaag
      421 gcgggcggga atctggtggt caagcttttt caaggcggcg gggagcagga gctgatgaag
      481 aggctcagaa ccctcttcac ccgggccaag atcgtgaagc ccaaagcgtg ccgcgacgac
      541 tcctttgaga tctatctggt cggagtcggc aggaaagaag gagaacaagg atgaagataa
      601 cggtaatcga cggccagggc ggcagcctcg gcaaagcgtt ggtggcggcg atcaagaaac
      661 ggtttcctca tgaagaggtc tttgcgatcg gaaccaacgc cctcgccgcc agcgcgatgc
      721 tgggaagcgg agcggatgca gcggcaagcg gggagaatcc ggtgttggtg gcatgtcgaa
      781 aaagcgaggt gattatcgga ccgatcggtc ttctcgccgc cgattcgctg cacggggaga
      841 tcaccccggc gatggctgta gcgatcggac aaagctccgc ccataagatc ctgcttccca
      901 tcaccaagtg caatgtatcg atcgtcggaa agcaggagct ttccaccaaa gagatgatcg
      961 agggggcggt cgccgaactt gcctcgtact tggttgaccg ttccctttaa gaacgctaca
     1021 ctcaaatcaa ccgctccatg aaggagcccc aaaagccatg aaggtaagga acgatcactc
     1081 tttctttgcg ttcatggctt tttcgttgga ggattatgtg actcgctatc aacaacccct
     1141 tgaagaaggt ctctcattgg ccgtatacaa cggggatgat ctgatcttta cccatcaagg
     1201 ccactggctc catcccctct tcgcgctcga ggagttcctc ataacgtatg aaggcccccg
     1261 gaatcttttg tccgttcacg acagcgccgc cggaaaagcc gctgcggttc ttcaagcgcg
     1321 gcttggagtg cgaagagccc acatcgatct gataagcgat ttggccgttg agtactaccg
     1381 ggcgaagggg atcgaagttt cctgggaaaa gaagatcgaa cggcttgcat gccagaccga
     1441 gagcctgctt tccgagatcg acgatgagga tgagatctat cgaatcctta aacgccgggc
     1501 gaaactggtt caaggtgtcg gtgttgaagt agccgggata agcttcgcct atccgaacag
     1561 tgaaccgctc ttcgagaatc tgcacttcac cctccccgaa ggagggcgcc tgatcatcca
     1621 aggggacaac gggatgggta aaaccaccct gatcaacctg ctgctcggca ggctgcttcc
     1681 cacggcgggg gcgatcagga tcgacggcag ggagccgggc gagcttccgc cccggacgat
     1741 cgggtatatc aagcagcaac agacccagga gcagtttccc gtttccgcgc gggaggtcgt
     1801 ggcgatggcc gtcgatccaa gcctccccag ggaacgacag gcgtgggaga tcgacactgc
     1861 cctccgaagg accgcttccg cccatctgag ctctcgaaac ttcttcagct taagcggcgg
     1921 tgaacgccag cgcgtctcgc tggcccgaac cctctgtcaa aaagcccgtc ttctcctgct
     1981 ggatgaaccc acctcgtttc tggatgcgaa aagccggacc acgctggtcg gcctgctcca
     2041 ctccctcacc cttgcggaga tgccgacgat catcatcgtc acccatgaca aggagctgga
     2101 agaggatctg gggtgggaga ttctgcgctt gggaggtgat catgaatgac tttctctttg
     2161 ccctcaccct cgccccggtc gcccgcggcc ttttggcaat ggcgatcgcc ggcctcgcgt
     2221 ttcccgccac gggggtcatg gtccttcggc tcaacctgat accgatgcgc tatatgctga
     2281 tgcacggggt gattctcgcc ggagcgatct cattggcgtt gaacgtaaac accttggcga
     2341 tcagcgtcgt gctgaacctc attctggtgc ttctgatgct caggctcaca aaggacaccc
     2401 gcttcggatt cggtctctcc agcgcgacgg caatggtctt cacgatggcc gcggcttcac
     2461 tggtgatgca catctgggat gtgccggcaa aagacaccct ccagctcctt tgggggtctc
     2521 ctttcgctct cacgtggacc gatatctccg ccctgatcgc tcttgcaatc atcatcgtcc
     2581 tctatatgtt catgagtttt cggacgatca gtgcgctctt cttcgaccag gagatcgcct
     2641 cctcgctggg gatgcgggtc cgtttccatc ataccttcat ggtaacactc atcgccctcg
     2701 tcatcgctct ggcgatgaag ctcctcggag cgctcctgat cgatgccttg ctgattcttc
     2761 ccgttctcgt cgcttccaaa cgaagcgaat cgctgaagcg gctcgtcctt tcggcgtgca
     2821 tcatcggctt ctttgtttcc accttgggat acctgttggc gatctacacc gacatgcccc
     2881 cgagcgggac aatcgccctg ctttccgccc tcatctatcc gataccgacc ttcaaaaaaa
     2941 ggagttgaat atgaaaagaa tcgtcgttct tatccttgca gcgctgttga gtttcaccct
     3001 cttcgcccaa gccgtcccgg aaaaacagga atccacggtc gtcgcctcga ccgcatggac
     3061 cgctgcgttt gccgatattg ccggagtcga caacgtcgac ttcatcgccc cggcgacgat
     3121 gactcatccc ccggagtatg agatcaccgt cggcgatgtc cagaagatca acaacgccga
     3181 gtacttcctc tacgcggggt acgaagtgat gatgaagtcg atgggaacga cgatcaagaa
     3241 ggacgagtcg gcccttatcc atatccaaac gaacaactcg attgaaaacg tccggaccca
     3301 aagcgccaag ctggctgcga tcttcggaac cgaaacggaa aacaaggttc gcttcgcgac
     3361 ctacgaagag gggattctcg acgggaaaaa gcaggtagcc gcaaagggtc tggataagaa
     3421 gaacgtctat tgccactcga tgcaggtgta cctggcgaaa gatctcggtc tcaacgtggt
     3481 cggaacgttc ggtccggctc cgctgacggc ggctcagctc gccgagatcg ccaaggggga
     3541 gatcgacatc atcatcgaca acatccacaa ccccgtcgct cctcccgcgt tggaggtttc
     3601 ccccaagagc aggatcgtaa cgtggcgcaa ccttcccggc cgtggcggac ggggaactct
     3661 tgaagagatg gttcgctcga atatcgccga actgcttaaa tgacgcagaa tgttcaagga
     3721 ggccgaagcc ggcctccttg ttccatctac atcgccttga tgcgggtaag gtcgccctcg
     3781 gccgctgccc ggagaagcgg cgccatatag gtgtccacat cggaggcatc catcggaatc
     3841 ggcatgttct gcagcttcat cttcagctgg gggtctttag cggcggcgag cgctcgggtg
     3901 atgtgggctt cggtgaaccc ttcgacatcg ctgagcttcg tcggggcatg gatcgacttg
     3961 gcaaaggcga tcatcgcttc agccacggaa accggagcgg catccgtgga accgaagatc
     4021 ggcccgatca cctgcaactg ctgcttgatc gcccggccga aga
//
LOCUS       DAOPUZ010000046         4040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_22419_length_4040_cov_2.924718, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000046 DAOPUZ010000000
VERSION     DAOPUZ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22419_length_4040_cov_2.924718"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /gene="ftsA"
                     /locus_tag="PKN79_01485"
     CDS             complement(<1..1040)
                     /gene="ftsA"
                     /locus_tag="PKN79_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNZ94465.1"
                     /translation="MAGEKMLMGLDIGSSRTRAVIGSVNREGQLMVDSICEHPSEGVR
                     SGSIVNIEQTLKTIQTVINEAELQAGAEISDVIVGIGGGHIIGIPSTGVVGISSPEQE
                     IKRGDIFRSLEVARAFELPQDREILHTLVQDFQIDGQSGIKDPIDMLGHRLESRVLIV
                     TASSAISQNQRKCIQRSGLNVARVVLQSLADAEVVLSTEEKEMGTILINIGSGTTDMI
                     AYRNGAPIFTGGVNLGGDDVTNDIAYILNKTRATAEQIKRESGHSYVPSVSNEEMIII
                     PQVGGMPAIKMPKRELSRIIEPRMAEIFSLLLDAFSKEHEGSGFGGGIVLVGGGALLS
                     GVTELASELFRMP"
     gene            complement(1061..1807)
                     /locus_tag="PKN79_01490"
     CDS             complement(1061..1807)
                     /locus_tag="PKN79_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94466.1"
                     /translation="MIRRITIYGITALLLSVLLFLALSHLPYFTISQVQVKIDGPLKH
                     VPIEISNRLSTLVGQGIFRVSLLSTKKQIEQLPIVTSLVLTRTLPSTIKATIAFIDSP
                     VIVQSENKERSYLVDGRTLVPIESSDADLWTDHLLRIEVPLEYALLMERYGTDSVFAS
                     VMGLTRGLSGEASLITRIKYDNNSSNSFGKMVLDLPSLNARLWVREPVDPKHVQTALY
                     WIEQDRKENLSFLSSSIQRYDLYREALVRR"
     gene            complement(1804..2961)
                     /locus_tag="PKN79_01495"
     CDS             complement(1804..2961)
                     /locus_tag="PKN79_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNZ94467.1"
                     /translation="MGSGYQNFDYQGSDPMPGSIEQPKSKSTVTFLIVTLLLTIFGLV
                     QLYSASYNEALLNGVPHYYYVVRQLQFVGLAAIGSVIIVFLPSKVLKAFAWPLALAAL
                     ALLLLTVFTPYGQEKFGSRRWLQIGPLPALQPSEFAKLALILLFASYQGKPKNILLPA
                     AVALLYAALVFLQRDYSTMLLIVVIAFSMLLYSGVRLAKVVLLLLFLIPPLFAALFSQ
                     AYRIRRVASFFFPSLDPSGMNYQVNVSLKAIAKGRLFGAGLGKGTYKLGILPEVHSDF
                     IIASVAEELGFVGILFILLLFFLYALLGYNGAKRMIGRDPLLSALSYGVTTMVLSQAI
                     LNLAVITALAPPTGIPMPFFSQGGTNMFVVLIASALILNVLRHANRKEEAR"
     gene            complement(2966..3874)
                     /locus_tag="PKN79_01500"
     CDS             complement(2966..3874)
                     /locus_tag="PKN79_01500"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNZ94468.1"
                     /translation="MGRRIVVVFLSFTITFVLSKVLLSLYRGQGTTAKAYLPDFHTNK
                     EGTPIIGGVAFIIGSSIATLFERDLFSPTILYPLIALLTFGGIGFFDDLLKERHRSGD
                     GFTSLGKLGLQLLVSILLVAVMPLRTTLTFRSMTLELGWWYYPFAVLFITSFVNAVNI
                     TDGLDGLASLSAIPSLLLLLSVQGAFSGALPGALIAFVWYNYPPAKMFMGDSGSHALG
                     AYIAVGALLANREVLILFAGGLFFLELFSSLVQIISIRGFGRKIFLIAPLHHALEAAG
                     FAEGKIVQTFVTLSWVFAILSFVMWG"
BASE COUNT         1013 a         1105 c         1078 g          844 t
ORIGIN      
        1 ggcattctga acaactcgct ggcaagctcc gtcactccgc tcagcagcgc tcctccaccg
       61 accagcacga ttcccccgcc gaaaccggaa ccctcatgtt ccttgctgaa cgcatccaag
      121 aggagggaga agatctccgc catccgcggc tcgatgattc ggctcagttc tcgtttgggc
      181 atctttatcg ccggcatccc cccgacttga gggatgatga tcatctcttc gttcgatacg
      241 gaaggaacgt aggagtgacc gctttcacgc ttgatctgtt cggcggtagc tcgagtctta
      301 ttcaaaatat aggcgatatc attcgttaca tcatcaccgc cgaggttcac ccctccggtg
      361 aagatcggcg ctccgtttcg atacgcaatc atgtcggtcg tgccgctgcc gatgttgatc
      421 agaatcgtgc ccatctcctt ttcttccgtg gagagcacga cttccgcatc cgccaggctt
      481 tggaggacga cccgggcgac attgagaccg ctgcgctgaa tgcacttgcg ctggttttga
      541 ctgatcgcgc tggaagcggt tacgatgagc acccggcttt ccagccggtg tccgagcata
      601 tcgatcgggt ctttgattcc gctctgtccg tcgatctgaa aatcctgcac gagggtatgg
      661 agaatctcgc gatcctgcgg aagctcgaac gcccgggcga cttccagact cctgaagatg
      721 tccccccgct tgatctcctg ctcggggctg ctgattccca cgacgccggt tgaaggaatc
      781 ccgatgatat gtcccccgcc gatgccgaca attacatcgc tgatctccgc tccggcttga
      841 agttccgctt cattgatgac ggtctggatg gttttaaggg tctgttcaat attgacgatc
      901 gatcccgatc gaactccctc ggagggatgt tcgcaaatgg aatcgaccat gagctggcct
      961 tcccgattga ccgatccgat caccgcccgg gttcggcttg accctatatc gagtcccatc
     1021 aacatttttt cacccgccat tgtcgtctcc ttggccacac ctatcgtcgc accaacgcct
     1081 ctctatagag gtcgtaacgc tgaatgcttg aactcaaaaa ggaaagattc tcctttcggt
     1141 cctgttcgat ccaatagagc gcggtctgaa cgtgcttcgg atccaccggt tcccgtaccc
     1201 aaagccgggc gttcaacgaa ggaagatcca aaaccatttt cccaaagcta ttactgctat
     1261 tattatcgta ttttatacga gttatcaagg aggcttcacc actgaggccc cgggtaagac
     1321 ccatcaccga agcgaagacg gaatctgttc cgtatcgttc catcaacagg gcatattcga
     1381 ggggaacttc gatcctcaac aggtgatcgg tccaaagatc cgcatcgctg ctctcaatcg
     1441 gaacaagggt ccgcccatcg accagatagg agcgttcttt gttctcgctt tgaacaataa
     1501 ccggcgaatc gatgaaggcg atcgtcgctt tgatggtcga cggcagagtt cgtgtaagga
     1561 ccaatgaggt gacgatcggc agctgttcga tctgcttttt cgttgacaag agcgacactc
     1621 gaaagattcc ctgtcctaca agcgttgaca ggcggttgga aatttctatg ggaacgtgct
     1681 tgagcgggcc gtcgatcttc acctggacct gggaaatcgt gaagtaagga aggtgggaaa
     1741 gtgcaagaaa gaggagcacc gaaagaagca gagcggtgat gccgtagatc gttattcttc
     1801 gaatcatctt gcttcctcct ttcggtttgc atgcctcagc acgttgagaa tcaaggcgct
     1861 ggcgatgaga accacgaaca tgttcgttcc cccctggctg aagaacggca tcggaatccc
     1921 cgtcggcgga gcgagagcgg taatcaccgc cagattcagg atcgcttggg agagaaccat
     1981 cgtggtaacg ccatagctca gagcggaaag aagcggatcg cgcccgatca tgcgcttcgc
     2041 tccgttgtat cccaagagcg catagaggaa gaagagcaga agaatgaaga ggattccgac
     2101 aaatccgagc tcctcggcga cggaggcgat gatgaagtcg ctgtgcactt ccggcagaat
     2161 ccccagttta tacgtcccct tgcccagacc tgcgccaaag agcctgcctt tggcgatcgc
     2221 tttcaagctg acgttcacct ggtagttcat tcccgaagga tcgagagagg gaaagaagaa
     2281 cgaggccacc ctgcgaatcc ggtaggcttg gctgaagagg gcggcaaaga gcgggggaat
     2341 gagaaacagg agcaggagca ccactttggc aagccggact ccgctgtaca ggagcatcga
     2401 aaaggcgatg acaacgatca gcagcattgt cgagtagtcc cgctgcagaa aaaccagagc
     2461 cgcatacagc aacgccactg ccgccggaag gagaatattc ttcggtttcc cctgatagga
     2521 ggcaaagaga agaatcaatg caagcttggc gaactcactt ggctgcaatg caggtagcgg
     2581 gccgatctga agccaccgtc tggagccgaa cttctcctgt ccatacgggg tgaataccgt
     2641 caagagcagc aaagccaatg cagcgagggc aaggggccag gcaaacgctt tgagaacctt
     2701 tgaaggcaaa aagacgatga ttaccgagcc tatagcggcc aatccgacga attgaagttg
     2761 acgaaccaca tagtaataat gcggtacacc gttgagcagc gcttcattat aggaggcgct
     2821 gtacagctga accaatccga aaatcgtcaa aaggagggtt acgatcagaa acgtgacggt
     2881 actctttgat ttcggctgct cgatcgatcc cggcatggga tcggagccct gataatcaaa
     2941 gttttgatac ccgcttccca tccccctacc cccacatgac gaaggagagg attgcaaaca
     3001 cccatgacag ggttacaaag gtttgaacaa tcttcccctc ggcgaatccg gccgcttcca
     3061 aggcgtggtg gagcggcgcg atcaaaaaga tttttctacc aaatccccga atcgatataa
     3121 tttgaacaag tgaggaaaag agctccagaa agaacaggcc tccggcaaag aggatcagaa
     3181 cctcccggtt ggccaagaga gcaccgacgg cgatatacgc acccagggca tgactgccgc
     3241 tgtcccccat gaacattttt gccggcggat agttgtacca gacaaaggcg atgagagccc
     3301 ccggcaacgc accgctgaag gctccctgaa cggaaagcag gagaagcagg gagggaatcg
     3361 ccgagaggct cgccagcccg tcaagaccat cggtaatgtt caccgcattg acaaacgagg
     3421 tgatgaacaa gaccgcaaac gggtaatacc accatccaag ctccaacgtc atcgatcgaa
     3481 acgtcaaggt tgttcgaagg ggcatcacgg cgacaagcag gatcgaaaca agaagctgca
     3541 atccgagttt cccaagagac gtgaatccgt cgccgctcct atggcgttcc ttaagcaggt
     3601 catcgaaaaa gccgattcct ccgaaggtca agagagcgat gagcggataa agaatggtcg
     3661 gagaaaaaag atctcgttca aagagtgtgg cgatcgaaga accgatgatg aacgcgacgc
     3721 ccccgatgat gggagttcct tctttgtttg tgtggaaatc cggtagatac gcttttgctg
     3781 tcgttccctg accccggtac aaagacaaaa gaaccttcga aaggacgaag gtaatcgtaa
     3841 aggaaaggaa tacgacgacg attcttctac ccatgcaggg cgaccccccg ttcgatcgtc
     3901 ggaatcaggc gttccatttg cacgacccgc gaacctttga ggaggacgag gtcaccggcg
     3961 gtgacctcgt ctctgaccat cccttcaagc tcctcaaaat cttgcgtgaa ggagactcgc
     4021 tccttaccct tctctctctt
//
LOCUS       DAOPUZ010000047         3994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_22766_length_3994_cov_3.578319, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000047 DAOPUZ010000000
VERSION     DAOPUZ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22766_length_3994_cov_3.578319"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PKN79_01505"
     CDS             complement(<1..1180)
                     /locus_tag="PKN79_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HNZ94469.1"
                     /translation="MKQVDVKAIRIHPEDTVAVAVHPLKKGETIVIGDFAVTVLEDIG
                     GGHKVALTGTGKGEPVIKYGFPMGVATKDIAVGEHVHTHNTSTRLSEHDEYHYDLNVR
                     DAFQQEADRRAKRREDSIPTIEAFERTDGKIGIRNEIWIVPTVGCVNMTAEQLVKWAE
                     KNLSKNEHYEGTYAWTHPFGCSQLGEDHETTRTILAGLVHHPNAGGVLVLSLGCENNT
                     PESFKELLGEVDPKRVKFLTTQEVEDELEASHALIRELYDEMQNDVRTPVGMDRLIVG
                     FKCGGSDGYSGITANPLVGYFCDELTAMGGTGILTEVPEMFGAEQLLMNRAKDEAVYE
                     KIVLLIKDFKRYFVKHGQVVYENPSPGNKKGGITTLEEKSLGCIQKGGTAIITDVIPY
                     G"
     gene            complement(1167..2114)
                     /locus_tag="PKN79_01510"
     CDS             complement(1167..2114)
                     /locus_tag="PKN79_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNZ94470.1"
                     /translation="MNRLLERFMPLITIGGVVTGLFFHRVFAPLKSFVNPLFAFITFS
                     SALAISPKRFFGALTKIKPIVLFLLWANLVMPLLAFALASLFFTEANLVTGFVLLTSI
                     PTAMAGYIWSGIYHGDEPLSLVLILISTLLGPILTPLTVKLLAQKAVVIDTRAMIMSL
                     LVMIVLPSLAGILINHAVGDERTKKVTPSLKPFAKIALFAIVTINISRIAPTLIERAT
                     LAYVPIALLAAFLAFLGYPASHTLGKIARLSLKESKSLTFAASMRNISASMVLAINYF
                     PPITALPIIFGIVFQQSICAIMASVLYGPKRKEAAHETG"
     gene            2228..>2596
                     /locus_tag="PKN79_01515"
     CDS             2228..>2596
                     /locus_tag="PKN79_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94471.1"
                     /translation="MKKIALVLASIHTGSSVTLWKEIAELAEASGDQLFVFPGGRIAY
                     QENQEYLRNEIYALVNPDNVEGMLVWASALSGAVSIEEVEAYLYNEELPTVSIGVKRS
                     AWPCVAFDAYAGMQTVIHHVI"
     assembly_gap    2597..2606
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2607..>3994
                     /locus_tag="PKN79_01520"
     CDS             <2607..>3994
                     /locus_tag="PKN79_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ94472.1"
                     /translation="KIAFLRGPEHHDSAQSRYNAYIDTLQHYRIDVDPLLISDPVGWT
                     EGKRAVKQLLDERCLVPGRDFDTLVCSSDLMMFGAGKHLEQLGYITGRDYIITGFNDS
                     RESRLLKVGCTTARMPVVNLARISYSLLGQNLAGDDSLLYDITLPCPLIIRRSCGCTY
                     ALGDEEHARSILVNETRFAEWIIEHLEVTKEEEPLLDSFLAGDFGVLSQFARSYFDRE
                     GDPNHLTEMIHWYQKFFAKKSFDNERIMGDLLAIQDLVTHEHAYERDCQEKLLNNLKS
                     DLLTVRRIPALGAIMAQHLPDLGITDAYLMLDGEEGVTTLVGGYRPEGPIRTQEQFSS
                     RLLLPQHYSQALGKGVFVILPLSVDNQSIGYLLVRSTRFNAVVLEDVRTSLSSAIKGA
                     LLVESANKARDLAEKTERSHAEFFANISEALRAPLEEILEITSDVTIANHVHHLFNTI
                     DLSLSYTPEFTL"
BASE COUNT          875 a         1086 c         1134 g          889 t
ORIGIN      
        1 ctccgtaggg gatgacgtct gtgatgatcg ccgttccccc tttttgaata catccgaggc
       61 tcttctcctc aagcgtcgtg attccgcctt tcttgtttcc gggagagggg ttttcataca
      121 cgacctgacc gtgtttgaca aaataccgtt tgaagtcttt gatcaataaa acgatttttt
      181 catacactgc ttcatctttt gccctgttca tcaacagctg ctccgcaccg aacatctcgg
      241 gaacctcggt aaggattccg gttccgccca tcgctgtcag ctcatcacag aagtacccga
      301 ccagggggtt tgcggtgatg ccggagtatc cgtcggatcc tccgcatttg aacccgacga
      361 tgagccggtc cattcccacg ggcgtccgga catcgttctg catttcatca tacagctccc
      421 tgatgagggc atgggaggct tcaagctcat cctccacctc ctgagtggtg aggaacttca
      481 cccgcttggg atccacctcg ccgaggagct ccttgaagga ctcgggggtg ttgttttcac
      541 agccgaggga gaggaccagg acgccgccgg cgttcgggtg gtggaccaag cccgccaaaa
      601 tggttcgggt cgtctcatgg tcctcaccga gctgggagca tccgaagggg tgggtccacg
      661 cataggttcc ctcgtagtgt tcgtttttgg aaagattctt ctctgcccac ttgacgagct
      721 gttcggcggt catgttcacg caccctacag tgggaacgat ccagatctcg ttgcgaatgc
      781 cgatcttccc atcggtccgt tcgaaggcct cgatcgtcgg aatggagtct tcccggcgct
      841 ttgcccgccg gtccgcctcc tgttggaacg catcgcggac gttcaagtca tagtgatact
      901 catcatgttc gctgaggcgg gtcgatgtgt tatgggtgtg cacgtgttca ccgactgcga
      961 tatccttggt ggcgacaccc atcggaaaac cgtatttgat gacaggttct ccctttcctg
     1021 tcccagtcaa tgcgaccttg tgacctccac cgatatcctc gagcacggtc acggcgaaat
     1081 ccccgatgac gatcgtttct ccctttttga ggggatgaac cgctaccgcg acggtatcct
     1141 cggggtggat tctgatcgct ttcacatcaa cctgtttcat gtgcggcctc cttgcgtttc
     1201 ggcccataga gcacggacgc catgatcgca cagatcgatt gttgaaagac gattccgaag
     1261 atgattggaa gggcggttat cggagggaaa tagttgattg ccaacaccat ggaagcgctg
     1321 atgtttcgca ttgaggcggc aaacgtcagg cttttgctct ctttcaggga gagacgggcg
     1381 atttttccga gtgtatggga cgccggatag ccgaggaacg ccaagaaagc ggcgagcaga
     1441 gcgatcggaa cgtaggcaag cgtcgcccgc tcaataagcg tcggggcgat ccgggagatg
     1501 ttgattgtca cgatcgcaaa gagagcgatc tttgcaaacg gcttcaaaga aggcgtcacc
     1561 ttcttcgttc gctcatcccc tacggcatgg ttgatgagga ttcccgccaa ggagggaagg
     1621 acgatcatca ccagcaagct catgatcatc gccctcgtgt cgatcacaac ggctttttgg
     1681 gcgagcagct tcacggtcag cggagtaagg atcggtccca gcaacgtcga gatgagaatc
     1741 agcaccaggg aaaggggttc gtcgccatgg tagatccccg accagatgta tccggccatc
     1801 gccgtgggaa ttgatgtcaa aaggacgaag ccggtgacga ggttcgcttc ggtgaagaag
     1861 aggctcgcca aagcgaaagc gagtagcggc atgacgaggt tcgcccatag cagaaaaaga
     1921 acgatcggtt tgatttttgt caaggccccg aaaaaccgct tcggactgat cgccaatgcg
     1981 ctggaaaacg tgatgaaggc gaacagcgga tttacgaacg atttcagggg ggcgaacacc
     2041 cgatggaaga acagaccggt aaccacgccc ccgatcgtga tgaggggcat gaatcgttca
     2101 agcagcctat tcaaagagcc gagccgagcc tcaagggttt ggaaatgaaa cattgtttca
     2161 gagcatataa gcgctgccga atactgtcaa gatacacatt gggcatggta tagtcgggaa
     2221 tgagaagatg aaaaaaatag cccttgttct tgcctcgatt cataccggat cctccgtaac
     2281 gttgtggaaa gagatcgccg agctcgctga agcttcgggc gaccagctct tcgtcttccc
     2341 cggcggacgg atcgcctatc aggagaacca ggagtatctg cgaaatgaga tctatgctct
     2401 ggtcaatccc gacaacgtcg aaggaatgct ggtctgggca agcgccctca gcggtgcggt
     2461 atcgatcgaa gaggtggagg cgtatctcta caatgaagag ctgcccaccg tatcgatcgg
     2521 cgtgaaacgc agcgcctggc cctgcgtggc gttcgatgcg tatgcgggga tgcaaacggt
     2581 gatccaccat gtgatcnnnn nnnnnngaag atcgcctttc ttcggggtcc cgaacatcac
     2641 gattcagccc aaagccgcta caacgcatat atcgacacgc ttcaacacta tcgaatcgac
     2701 gtcgatcccc tcttgatcag cgatccggtc ggctggaccg agggaaaacg ggcggtcaag
     2761 cagctgcttg atgagcgctg tctggtcccg ggcagagatt tcgatacgct cgtctgctcc
     2821 agcgacctga tgatgttcgg cgccggaaag caccttgaac agctgggtta catcacaggg
     2881 cgcgattaca ttatcaccgg tttcaatgac agccgggaga gccgccttct caaagtcggc
     2941 tgcaccaccg cccggatgcc ggtggtgaac cttgcccgga tcagctactc cctgcttggg
     3001 cagaacctcg ccggcgacga ttcgcttctc tatgatataa cgcttccctg tccgctgatc
     3061 attcgccgct cctgcggctg cacctatgcc ctcggggacg aggaacacgc ccgctcgatt
     3121 ttggtgaacg agacccggtt cgccgagtgg atcatcgaac atctggaagt gacgaaggag
     3181 gaagaacccc ttctcgattc ctttctggca ggggattttg gcgttctttc gcaattcgcc
     3241 cgctcctatt tcgatcggga aggggatccg aaccacctga ccgagatgat ccattggtat
     3301 cagaagttct tcgccaagaa gtcgtttgac aacgaacgga tcatgggcga cttattggcg
     3361 attcaggatc tggtcaccca cgagcacgcc tatgagcgcg actgccagga gaagctgttg
     3421 aacaatctca aaagcgatct gttgaccgtt cgccggattc cggccctcgg ggcgatcatg
     3481 gctcaacacc tgccggatct gggaatcacc gacgcatact tgatgttgga tggggaagaa
     3541 ggagtgacga ccttggtcgg agggtaccga ccggagggtc cgatccgaac gcaggagcag
     3601 ttttcgagtc ggcttctcct ccctcaacac tacagtcaag cattgggcaa gggggtgttc
     3661 gtcatccttc cgctttccgt cgacaaccag agcatcgggt atctgttggt ccggtcgacc
     3721 cggttcaacg cggtggttct ggaagatgtg cgaaccagtc tcagcagcgc gatcaagggg
     3781 gcgcttctgg tcgagagcgc caataaagcc cgggatctgg cggaaaagac ggagcggagc
     3841 catgccgagt tcttcgcgaa catcagtgag gcgcttcggg ctcctttgga agagattctt
     3901 gaaattacaa gcgatgtgac gatcgccaac catgttcatc acctcttcaa tacgatcgat
     3961 ctgagccttt cctatacccc cgagttcacc ttgg
//
LOCUS       DAOPUZ010000048         3993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_22779_length_3993_cov_2.624175, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000048 DAOPUZ010000000
VERSION     DAOPUZ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22779_length_3993_cov_2.624175"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..930
                     /gene="miaA"
                     /locus_tag="PKN79_01525"
     CDS             28..930
                     /gene="miaA"
                     /locus_tag="PKN79_01525"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNZ94473.1"
                     /translation="MLFGPTAVGKTAILSRFDSSRYSVINADSIQVYKGLDIGSAKAT
                     GAEREAIEHHLIDIREMDQSFSVGDFIEAADEAVALIHSQNKIPIISGGTAFYVKHFL
                     YGLSEAPSSNEDVRACVGALIEEKGLSWGYKRLAEVDPVSADRIHPNDTYRISRALEV
                     YEQSGKPLSSFDLPSEKRISNPILIIGLERERQELLDRLKRRIKQMETDGLVEEIRSL
                     LRQGAQSTWASMAGIGYREFFELSESGEYSVQSVLRRIERNTKLYTKRQMTFFRSFAA
                     AEWFGADDVDSIIERVEAFVRGEG"
     gene            complement(890..2362)
                     /locus_tag="PKN79_01530"
     CDS             complement(890..2362)
                     /locus_tag="PKN79_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94474.1"
                     /translation="MKLYQPFNTTYNDEEITAIPFASAAVELPTFHLQGERAASHLYK
                     DGKLTTWYWQGIALIENQRSVYFPRQNLFSFTELATVRRSKALSWVRRLAKALTLTTG
                     SFLDLSSGILPLWRIYGGEDGSILILSQDLGDLFAAMANDEPKFFNISAWVHHNIHPA
                     FTLCDQLTQLLYYAITGTPPFLRKETREDRYRHLPLEYSFEEVSLQTRLFIDASLNLS
                     LTKQRDSTGNKEPQKALTAFLDATESIEWDAENRTEVPAFSSWQNTPKVQEFLASQAK
                     RAKRIVFWRMKGWLIITIAVSVILVSWFTIDRVSEALKPPYTQFMDPPAIITEYYKGL
                     NALDLSHMDAALAKKVKNPWTMEVTNLFVTRQTRKAYEGFSPTVDPNEWFAGGQKPLI
                     EGSFLYGTTDVTITRLDGRTFEAQAILYTPYPYDEEEADQEHRQSGSYMYTLVQTFTL
                     EEGKKGWYEITSISPPRITRIGFLEIESIPRLEQTPPPAR"
     gene            complement(2367..3176)
                     /gene="amrB"
                     /locus_tag="PKN79_01535"
     CDS             complement(2367..3176)
                     /gene="amrB"
                     /locus_tag="PKN79_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNZ94475.1"
                     /translation="MTTYHHTIFYPEEREPLDEATYKRSEAGTLKSLPSALLLPHAAY
                     SMIGEALHQTFAHASSLKPELVVFLCPLHQPVLDRDKPAFLFAPAADTFEQAGRTYQF
                     DEAMIGELTLGFPIRRAASYYEEEPAFELTLPFIDSYLTPRAILPLIGKVENSNQIAQ
                     LILALKTIVQRQPKVLFVLSANASELLPQREATDEANHFIEDLIHEHQSLGIRSSCNW
                     GMIEAMNRSALVKGRWKIASAFLKGTDHGRITDTKNESGLMTWHIGAYKGV"
     gene            complement(3176..>3993)
                     /locus_tag="PKN79_01540"
     CDS             complement(3176..>3993)
                     /locus_tag="PKN79_01540"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HNZ94476.1"
                     /translation="LLYEKKARSLEHTLASLNTRIDQTRDFERYKHLGDLLSSNQHLI
                     REGQTSLSVHDWESGEEVEIPLDPTRSPRDAIQLYYESYQRSKGRYENALGELKRVQE
                     ELRAHQATFERALDRSLPQEEALALLTSLVEESEKEPIVRPSVGLVFQSGAYTLLVGR
                     NAKENDELLRRHTRGNDWWVHTRDVPGGYVFIKYIKDKPPPLEVLLDAAYLAILYSKA
                     KKAGKAELYYTQVKYLRRAKGAKMGTVLPTQERNLSVTLDKARLNVLLAQGEV"
BASE COUNT          867 a         1002 c         1154 g          970 t
ORIGIN      
        1 acctctgaga ctatcaaacc gattatcatt ctctttggac cgactgcggt aggaaaaacc
       61 gccattctca gtcggttcga ctcgtcacgc tacagcgtca tcaacgccga ttccattcag
      121 gtctacaagg gcttggatat cggatcggcg aaggcgaccg gagcggagcg tgaagcgatc
      181 gagcaccacc tcatcgatat tcgggagatg gatcaatcct tttccgtcgg cgatttcatc
      241 gaagccgccg atgaagcggt agcgttgatt cacagccaaa acaagattcc gatcatcagc
      301 gggggaacgg cgttctacgt aaaacacttc ctctacggtc tgagtgaggc accctcaagc
      361 aacgaggatg tgcgtgcctg tgttggcgcc ctcattgaag aaaaaggtct gtcttggggg
      421 tacaagcgcc ttgcggaagt cgatccggtc agtgccgatc gaattcatcc gaatgacaca
      481 tatcgaattt cccgggctct tgaagtgtat gagcaaagcg gcaaacccct ctcttcgttc
      541 gatcttccaa gcgagaagag gatctccaat cccatcttga tcatcggctt ggaacgggag
      601 cggcaggagc tgctggatcg gcttaaacgg cggatcaaac agatggaaac cgatggcctc
      661 gtcgaagaga ttcgctcgct ccttaggcaa ggagcccaaa gcacatgggc ctcaatggcg
      721 gggatcggat accgggaatt ttttgaactt tccgaaagcg gcgaatattc cgttcaatcc
      781 gttcttcgcc ggatcgagcg caatacgaag ctgtacacga agcggcagat gacgtttttc
      841 agaagctttg ccgctgccga gtggtttggt gccgacgacg tcgattcaat catcgagcgg
      901 gtggaggcgt ttgttcgagg cgagggatag actcgatttc aagaaagccg atgcgagtga
      961 tccggggtgg agagatggag gtgatctcat accacccttt ctttccctcc tcaagggtga
     1021 atgtctggac gagggtgtac atatacgatc cgctttgccg atgttcctga tcggcctcct
     1081 cttcgtcata gggatacggg gtatagagaa tcgcttgtgc ttcaaacgtt cttccatcaa
     1141 ggcgggttat cgtcacatcg gtcgttccat agaggaaaga gccttcaatc aagggttttt
     1201 ggccgccggc aaaccattca ttgggatcga ccgtgggcga gaacccttca tatgctttgc
     1261 gggtctgtcg cgtgacgaac aggttggtga cctccatcgt ccaagggttt ttcaccttct
     1321 tcgccaaagc cgcatccatg tgggaaagat caagagcatt cagtccttta tagtactcgg
     1381 tgatgatcgc ggggggatcc atgaactgag tatagggagg tttcagcgct tcgctgaccc
     1441 gatcgatcgt aaaccacgaa acaaggatga cgctgacggc gatcgtgatg atgagccatc
     1501 ccttcattcg ccagaagacg atccgtttgg ctcgtttggc ttggctcgca agaaactcct
     1561 gaaccttcgg ggtgttctgc caggaggaga aggccggcac ttccgttcgg ttttccgcat
     1621 cccactcgat cgattcggtg gcatccagaa acgccgtgag ggctttttgc ggttccttgt
     1681 tccccgtgct gtcccgctgt ttcgtcaggg aaaggttcag gcttgcatcg atgaaaagcc
     1741 gggtctgaag agaaacctct tcaaagctgt attcaagcgg aagatggcga tagcggtctt
     1801 cccgggtttc tttgcgtaaa aatggagggg ttcccgtaat tgcatagtag agcagctgcg
     1861 tcagttgatc gcagagggtg aatgccgggt gaatgttatg atgaacccac gccgagatgt
     1921 tgaagaattt cggttcatcg ttcgccatgg cggcgaagag gtcgccaaga tcctggctga
     1981 ggatgaggat cgagccgtcc tcgcctccgt agatccgcca caggggaaga attccgctgg
     2041 agagatcgag aaaactgccg gtggtgaggg tgagcgcctt ggccagacgc ctgacccacg
     2101 agagcgcttt gcttcggcga acggtcgcca gttcggtaaa ggagaagagg ttttggcggg
     2161 gaaagtagac ggagcgttgg ttctcgatca gggcgattcc ctgccagtac cacgtcgtaa
     2221 gctttccgtc cttgtagaga tgggaagcgg ctcgttcccc ttgcaggtgg aacgtcggca
     2281 gctcgaccgc tgcgctcgca aaggggattg cggttatttc ctcatcatta taggtcgtat
     2341 tgaacggttg gtagagcttc atctccctaa actcctttgt acgccccgat atgccacgtc
     2401 atcagtcccg attcgttttt ggtgtccgta attctgccat ggtcggttcc tttcagaaac
     2461 gccgaagcga tcttccatct tcctttgacc agtgcgcttc gattcattgc ttcgatcatg
     2521 ccccagttgc aggagctccg aattcccaac gattgatgtt catgaatcag gtcctcaatg
     2581 aagtgattcg cttcatcggt cgcttcgcgt tggggcagca gctcgctcgc atttgccgaa
     2641 agaacgaaca acacctttgg ctggcgttgg acgattgttt tcaacgcaag aatcaattga
     2701 gcgatctgat tggaattttc aacctttccg atcaagggaa ggatcgctct gggcgtaagg
     2761 tagctgtcga tgaagggaag agtcaattca aaggcaggct cctcttcata ataggaggct
     2821 gcccggcgaa tcgggaaccc caaggtcaac tcaccaatca tcgcttcgtc aaactgatag
     2881 gttcgtcccg cttgctcaaa ggtatcagcg gcgggggcaa agaggaacgc gggcttgtcc
     2941 cggtcaagga ccggttgatg aagggggcag agaaagacga cgagctccgg tttcaagctt
     3001 gatgcatggg caaacgtctg atgaagagcc tccccgatca tcgaataggc ggcgtggggg
     3061 agcaggaggg cggaagggag gcttttgagg gttcccgcct cgcttcgctt atatgtagct
     3121 tcatccagcg gttcccgttc ttcaggataa aaaatcgtat ggtgataggt cgtcactaca
     3181 cttctccttg cgccagaagg acattcaagc gcgctttatc cagtgtaaca gaaagatttc
     3241 gctcttgggt aggcaaaacg gtgcccatct tcgctccttt cgcccggcgc aagtatttga
     3301 cttgggtgta gtagagttcc gctttccccg ctttttttgc tttcgaatag agaatggcga
     3361 gatacgccgc atcaaggagg acctccagcg gcggcggttt atccttgatg tatttgatga
     3421 atacatagcc gccgggaacg tcgcgggtgt ggacccacca atcgttgccc ctggtgtgcc
     3481 ggcgaagcag ttcatcgttt tctttagcgt ttcgaccgac gagcagcgtg tatgccccgc
     3541 tttgaaagac caggcccacg ctcgggcgaa caatcggttc tttttccgat tcttcgacca
     3601 gactcgtcaa caaagcaagc gcttcttctt ggggaagact ccgatcaagc gcccgttcaa
     3661 aggtcgcctg atgggctctg agctcctctt gtacccgttt cagctcgccg agggcgtttt
     3721 cataccgacc tttcgagcgt tggtagctct cgtagtagag ctggatggca tcgcgcggtg
     3781 atcgggtggg atcgaggggg atttcaactt cttcaccgct ctcccaatca tggacggaca
     3841 gggaggtttg accttcccga atgaggtgtt gattggaact gaggaggtct ccgaggtgct
     3901 tgtatcgttc gaagtcccgg gtttgatcga ttcgggtgtt cagggaggcg agagtgtgct
     3961 caagggagcg ggcttttttc tcgtagagaa gcg
//
LOCUS       DAOPUZ010000049         3953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_23121_length_3953_cov_3.363776, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000049 DAOPUZ010000000
VERSION     DAOPUZ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23121_length_3953_cov_3.363776"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKN79_01545"
     CDS             complement(<1..346)
                     /locus_tag="PKN79_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNZ94477.1"
                     /translation="MYNFKTLYEATSVAEALKLKADHPEALFLAGGSDILIKIREGRL
                     DGVDLINIYMLDELRGICLEDDGSILIRPLTSFTDVAMHPVIKEHLPVLGEAVNTIGG
                     PQVRNIGTIGGNI"
     gene            complement(339..2645)
                     /gene="xdhA"
                     /locus_tag="PKN79_01550"
     CDS             complement(339..2645)
                     /gene="xdhA"
                     /locus_tag="PKN79_01550"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HNZ94478.1"
                     /translation="MKIIGSSPNRVDAFDKVTGKAKYAPDLLPPHALEAKVLHSTIAN
                     GWVREFDLSEAWKVEGVVDIVTCFDVPDIQYPTAGHPWSNVPTKQDICDRKLLNTRVR
                     QYADDIAAVIAETELAAIEAVRKIKVVYEELPVILDVKESLENTEFPLHEERPDNIVN
                     KTSYEIGDFEEAIKEKGLTRISGTYETPVVSHCHIEPANSFAYEEAGKIVVVSATQIP
                     HLVRRMVGQALGVDWGKVRVIKPYIGGGFGNRQDALTEPLNAYLTTRVGGRPVRLAYS
                     REETFECTRTRHAMTFDLTTYVRKDGSFAARELTVYSNQGGYASHGHALAANAANAFR
                     MMYPVGAIRGKAITVYTNMPTAGAMRAYGIPQIAFALESHIDDIGKATGLDPLLLRTK
                     NMMPLGYVDGPTTITCHSTGLAECIAKGEEHLDYSKKRLAYANQDGPIRRGVGMAIFC
                     YKTGVYPISLETSAVRMVLNQDGSMQVQVGATEIGQGADTMWVMMTAETVGVTLDKVY
                     LVSKQDTDVTPYDTGAYGSRQTYVSGMAVKKTAESMRRKILEYAALMTNKDPMDLDIV
                     ANKIVRKNTDLVLTTVKDVAMESCYSLTNSNHIAVEETHHCSDNTYAFGVCFTEIEVD
                     IPLCQIKVLDIINVHDSGRIINQTTARGQVHGGMSMGLGYGLYEYHKFDPKTGRMLNG
                     NLLDYKLMTPLDTPTLHAAFVETDDPTGPYGNKSLGEPPTIPVAPAIRNAVLNATGVS
                     INANPLHPERLFDSFVEAGLIKQRSEHV"
     gene            complement(2881..>3953)
                     /locus_tag="PKN79_01555"
     CDS             complement(2881..>3953)
                     /locus_tag="PKN79_01555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNZ94479.1"
                     /translation="SIVVLWRNYGTDHVHYVRQKLLLTFVLLLPGLTNLLYNFNLSPV
                     RGLNLSTATLTVSGVLFYIILYRNSLLDLLPVTREQIVENLDEAVLIFNPQKRLIDFN
                     HSAAKLFNLGRNSIGTGVEEIGVKELTHRLENLQDAESKTLALMQGLVYDLSLNFVRD
                     PSSEFCQAIIVTLRDITQEQLMNQRLQILAERDSLTGLYNRHTFFFRSKELIKTNPRV
                     RIAAIMIDLDNFKAINDTYGHAYGDLILQTMGEALLALASPTIVAGRLGGDEFVVMTT
                     EQDLSDLASRLSKKVYDRLNVVVKEGLNPTLSVGVSSLNLASCTVEDPLEYLLNQADL
                     AMYQAKRGGKDKIVFTGDFIPD"
BASE COUNT          837 a         1052 c         1125 g          939 t
ORIGIN      
        1 agatgttccc cccgatcgtc ccgatgttcc gcacctgagg tccgccgatc gtgttgaccg
       61 cttcgccgag aaccggaagg tgctccttga tcaccgggtg catcgccacg tcggtgaaac
      121 tggtgagcgg gcggatgagg atcgagccgt catcctccag gcagatcccc ctcagttcat
      181 ccagcatgta gatgttgatc agatcgactc cgtcaaggcg gccttcgcgg atttttatga
      241 gaatatcact gccgccggca agaaagagcg cctcggggtg atccgctttg agcttgaggg
      301 cttcagcgac ggatgtcgct tcgtagaggg ttttgaagtt atacatgctc actcctttgc
      361 ttgatcagcc ccgcttcaac gaacgagtcg aagagccgtt cggggtggag ggggtttgcg
      421 ttgatcgaaa cccccgtcgc attgagcacg gcgtttctga tcgccggggc gacgggaatc
      481 gtcggaggtt cgcccagact cttgttaccg tagggaccgg tcgggtcatc cgtttccacg
      541 aatgccgcat ggagggtcgg agtatccaac ggcgtcatca gcttgtaatc gaggaggttg
      601 ccgttgagca tccgtcccgt cttggggtcg aacttgtggt actcatacaa cccatacccc
      661 agacccatgc tcattccgcc atgaacctga ccgcgggcgg tcgtctgatt gatgatccgt
      721 ccgctgtcat ggacgttgat gatatcgaga accttgattt gacagagggg aatatcaacc
      781 tcgatttccg tgaagcagac tccgaatgcg taggtgttgt cgctgcagtg atgggtttcc
      841 tcgacggcga tgtgattgga gttcgtcaag ctgtaacacg actccatcgc aacatccttg
      901 acggtggtca ggacgaggtc ggtgttcttc cgcacaatct tgttggcgac gatatcgaga
      961 tccatcggat ccttgttcgt catcagggcg gcgtattcaa gaatcttgcg ccgcatcgat
     1021 tcggcggttt tcttcaccgc cattcccgat acgtatgtct gtctcgatcc gtatgctccg
     1081 gtatcatagg gagtcacatc cgtatcctgt ttgctgacaa ggtacacttt atccaaggtg
     1141 acgcccaccg tttcggcggt catcatcacc cacatcgtat cggctccctg gccgatctcg
     1201 gtcgctccga cctgcacctg cattgatccg tcctgattga gaaccatccg aaccgcggag
     1261 gtctcaaggc taatcggata gacgccggtc ttataacaga agatcgccat gccgacgcct
     1321 cggcggatcg gaccatcctg attggcgtag gcgaggcgct tcttcgaata atccagatgc
     1381 tcctcgcctt tggcgatgca ctcggcaagt ccggtggagt ggcaggtgat cgttgtcggt
     1441 ccatccacat agccgagggg catcatgttc ttcgtccgaa gcaggagggg atcgagtccc
     1501 gtagctttgc cgatgtcatc aatgtgcgac tccaaggcga aggcgatttg aggaattcca
     1561 tatgctctca tcgctccggc ggtcggcatg ttggtataga cggtgatcgc tttgccgcgg
     1621 attgctccga ccggatacat catcctgaag gcgttcgccg cgttcgccgc aagggcatgg
     1681 ccgtgggagg cgtagccgcc ctggttggag tagaccgtca gttcgcgggc ggcgaaggat
     1741 ccgtccttcc gaacataggt ggtcagatcg aaggtcatcg catggcgggt tctggtgcac
     1801 tcgaacgttt cctccctgct ataggcgagc cgaaccggcc gtcctcccac ccgagtggtg
     1861 aggtaggcgt tcagcggttc ggtaagagcg tcctggcggt ttccgaatcc gcctccgata
     1921 tacggtttga ttacccgaac ctttccccaa tcgactccga gggcttgccc gaccatcctt
     1981 cggacgaggt ggggaatctg cgtcgcactg acgacgacga tttttcccgc ttcttcgtag
     2041 gcgaacgagt ttgccggctc gatgtggcag tgggagacca ccggggtctc gtaggtgccg
     2101 gaaattctgg tcaacccctt ttccttgatc gcctcttcaa aatccccgat ctcgtaggag
     2161 gtcttgttga cgatattgtc cggtcgctcc tcatggaggg gaaactcggt gttttcaagg
     2221 gattccttta catccagaat caccggaagc tcctcgtaaa cgaccttgat cttcctgacg
     2281 gcttcaatgg cggcaagctc cgtctcggcg atcaccgccg caatatcatc ggcgtactgg
     2341 cggactcggg tgttgagcag cttgcgatca cagatatcct gttttgttgg aacatttgac
     2401 caggggtggc cggcggtagg atattgaatg tccggtacat caaagcaggt aacgatgtct
     2461 acgacccctt caaccttcca cgcctcgctg agatcgaact ctctgaccca gccgttggca
     2521 atcgtggagt gaagcacttt cgcttcaagg gcatgaggtg gaaggagatc gggagcatat
     2581 ttcgctttac cggttacttt gtcaaacgca tcgactcgat taggtgaaga accaataatc
     2641 ttcatgcaac cctccctgca ctctgtcatc catcatgtga tgagcataca aagaaccgaa
     2701 gcggccggaa gacagagtgc tctttttcct cccttcataa tacaacagat tgcaacaaat
     2761 tcaagaaaat acgaaaaaaa taagcaacat tcatgaggga taaaccatcc taattatcag
     2821 gggaggatat ggcgccgaac accgtattcg aggaacccga caaaacgagc aatggaagcg
     2881 ctagtcggga atgaagtccc ctgtaaagac tatcttgtcc tttccccctc gtttggcctg
     2941 gtacatcgcc agatccgctt ggttgagcag gtattcgagg ggatcctcta cggtgcatga
     3001 tgcaagattg agggagctga ctccgactga aagagttggg ttcagtcctt ctttcaccac
     3061 gacattcagt cgatcgtaaa cctttttcga caggcgtgag gcaagatccg acagatcctg
     3121 ttccgttgtc atgacgacaa actcatcgcc gccgagccgt ccggcaacga tggttggcga
     3181 ggccagggca aggagcgctt cccccatcgt ctggagaatc aagtcaccgt aggcgtggcc
     3241 gtaggtgtcg tttatcgctt tgaagttgtc aagatcgatc atgattgcgg cgatgcgcac
     3301 tctcgggttg gtcttgatca gttccttgct ccggaagaaa aaggtatggc ggttgtataa
     3361 cccggtgagc gagtcccgtt cggcaaggat ttgaagccgt tgattcatca gttgttcctg
     3421 ggtgatgtcg cgaagcgtca caatgatcgc ctggcagaat tcagagctcg gatcccggac
     3481 gaaattgaga ctgagatcgt agaccagacc ttgcatgagg gcgagggtct tggactccgc
     3541 gtcctgaaga ttttcaagtc ggtgagtcag ctctttaact ccgatttctt cgactcctgt
     3601 cccgatcgag ttgcggccca aattgaacag cttcgccgcc gaatggttga aatcgatcag
     3661 tcgcttctgc gggttgaaga tcaagaccgc ttcgtccaga ttttcaacga tctgttcgcg
     3721 ggtgacgggc agcaggtcca acagcgagtt gcggtagagg atgatgtaga acagaacgcc
     3781 gctgaccgtc agagtggccg ttgaaaggtt caggcccctg accggactga gattgaaatt
     3841 gtacagcaga ttggtcagac ccggaagaag gaggacgaag gtgagcagga gtttctggcg
     3901 cacatagtgg acgtggtcgg ttccatagtt ccgccaaaga acgacgatcg agg
//
LOCUS       DAOPUZ010000050         3921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_23391_length_3921_cov_3.459907, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000050 DAOPUZ010000000
VERSION     DAOPUZ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23391_length_3921_cov_3.459907"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="PKN79_01560"
     CDS             <1..1638
                     /locus_tag="PKN79_01560"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNZ94480.1"
                     /translation="TEESGKVYGFNLVYSGNHRELVEVSPFGKTRVMTGINPTGFSWT
                     LTSGERFQTPEAVMTYSPHGLEGASQNFHQFINHHIIRGPWKFRERPILINNWEATYF
                     NFTEDKILKLARESAELGIELFVLDDGWFGVRNDDTTSLGDWTVNSKKLPSGLGYISS
                     QIKKMGMLFGLWVEPEMVSIQSELYKNHPDWMIAIPGRTPGVGRNQYILDLTREDVRR
                     YLFETLSKTWKLADVNYIKWDMNRVFSDLYSLNPEMANHGEFLHRYVLGLYELLTKLA
                     TEFPAVLFESCASGGNRFDLGMLCFMPQTWTSDNSDALSRLYIQEGTSYAYPLSTMGA
                     HVSSSPNHQTLRRTDLESRFNVAAFGVLGYELDVTSLNRQQKEAIKAQIAFYKAHRSI
                     LQYGTFTRIKLANSAANQVVWAVASADRSELLVLFAQKLNQANPGGDILRVHAVDLNA
                     TYEVFPRQQKIDIKVFGDLLNMVSPVHITEGGIAQETISKALSLDSEVEHYRLSGEQI
                     AYGGIKLNQQFGGTGYDSMTRVLGDFGSRIYLFKRID"
     gene            1668..2135
                     /locus_tag="PKN79_01565"
     CDS             1668..2135
                     /locus_tag="PKN79_01565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ94481.1"
                     /translation="MSVTIYYLKKGDDHPSYLLHCQAIRRVVFVEGQGVPEAIDFDGQ
                     DGSSGHLLLFDTNIPVGTTRIRRTDKGMKLERIAVLPAYRKKGYGELLVKAALSKATG
                     TIYINAQVASLGFYEHLGFVREDKTTFLEAGIPHTVMLWPYGQSEAPCPILEP"
     gene            complement(2189..3253)
                     /locus_tag="PKN79_01570"
     CDS             complement(2189..3253)
                     /locus_tag="PKN79_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ94482.1"
                     /translation="MKKRIAKISVVLAALLFLVMLPLAAGGGKEEKATDPTAMEKNLV
                     LYSSMTENDLSNLVRLFNKQYPDVKIEIVNGSAGELTSRIRAERNNPQGDLMWGGLSS
                     SDGMVHSDLFEHWLTEYEPDIMPDYRSNNGFYSLDHLSTAVFCVNVELEKELGLNIRG
                     YKDLLDPRLKGKIVMPDPNSSSSGWNNVSNMMAVFGHGTPEVWDYITKFMNNQVVFST
                     ASSVAFKSVDTGEYVVGLTYEDGASTLLKAGSNRIRMVYPEDGASASAFGCALIAGAK
                     NPNAAKAMINYLMSAEGQSELGMALETLRFTNEKAVYETPYLPASSEIKWVTRDNDWL
                     IANKKAVLEQWNKIYANVKK"
     gene            complement(3297..>3921)
                     /locus_tag="PKN79_01575"
     CDS             complement(3297..>3921)
                     /locus_tag="PKN79_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ94483.1"
                     /translation="LLFRSIKNTLVLGFGALAIIIVFSVLIAYLVVRRPSWINNVIDT
                     LSMLPYIMPGAIIGIALVVAFGQPPLVLSGTMAIMIISFVIRRIPYSIRSTNAILMQI
                     PISVEEAAISLGASKLKTFVVITVPMMVNGIISGAILSWVAIVTELSSSIILYNNRNI
                     TLTMSTYVAISRGSYGLACAFSAILTLFTTVSLLLYLVISKTEDIRL"
BASE COUNT          904 a         1162 c         1049 g          806 t
ORIGIN      
        1 acggaggaga gcggaaaggt ttatggcttt aacctcgtct acagcggcaa ccaccgggag
       61 cttgttgaag tctccccgtt cgggaagacc cgggtcatga ccggcatcaa ccccacgggc
      121 ttcagctgga cgctcacaag cggcgaacgc ttccagaccc ctgaagcggt catgacctat
      181 tccccccacg gactcgaagg ggcctcgcag aacttccacc aattcatcaa tcaccacatc
      241 atccgcggac cgtggaagtt ccgcgagcga ccgattctga tcaacaactg ggaagccacc
      301 tacttcaact tcaccgaaga caagattctg aagctcgccc gggaaagcgc cgagctgggc
      361 atcgagcttt tcgtccttga tgacggctgg ttcggggtcc gcaacgacga caccacgagc
      421 ctgggcgact ggaccgtgaa ctccaagaag ctgccctcgg gcctcggata tatttcatca
      481 caaatcaaaa agatggggat gctcttcggc ctctgggtcg agccggagat ggtctcgatc
      541 cagagcgaac tctacaagaa ccaccccgac tggatgatcg cgattccggg gcggacgccc
      601 ggcgtcggcc ggaaccagta catcctcgac ctcacccggg aggatgtccg caggtatctc
      661 ttcgagaccc tttcaaagac gtggaagctc gccgacgtga actacatcaa gtgggacatg
      721 aaccgcgtct tcagcgacct gtacagcctc aatcctgaga tggccaatca cggggagttc
      781 ctccaccgct acgtcctcgg cctctatgaa ctgctcacga agctcgccac cgagttcccg
      841 gcggtcctct tcgaatcgtg cgccagcggc ggaaaccgct tcgacctggg gatgctctgc
      901 ttcatgcccc agacgtggac cagcgacaac agcgacgccc tctcccgcct ctacatccag
      961 gaggggacca gctacgccta tcccctctcg acgatgggag cacacgtttc cagttcgccg
     1021 aaccaccaga cgctccggag gaccgatctg gaaagccgct tcaacgtagc ggcgttcgga
     1081 gtgctcggct atgaactgga cgtcacctcg ctgaaccgcc agcagaagga ggccatcaag
     1141 gcccagatcg ccttctacaa ggcccaccgt tcgatcctgc agtacggaac cttcacccgg
     1201 atcaagcttg cgaacagcgc cgccaaccag gtggtctggg cggtcgcttc agcggacagg
     1261 agcgaactcc tggtcctgtt cgcccagaag ctgaatcagg ccaaccccgg aggcgacatc
     1321 cttcgagtcc atgcggtcga tctcaacgcg acgtacgagg tgttccccag acagcagaag
     1381 atcgacatca aggtgttcgg cgacctgctc aacatggtct ccccggtcca catcaccgaa
     1441 gggggcatcg cccaagagac gatcagcaag gcgctctccc ttgacagcga ggtcgagcac
     1501 taccgcctca gcggcgagca gatcgcctac ggagggatca agctgaacca gcagttcggc
     1561 ggcaccggct atgattcgat gacaagggtt ctgggggact tcggttcgcg gatctacctc
     1621 ttcaagcgaa ttgactgaac ccgccccacc tttgtacact cccactcatg agcgtcacga
     1681 tctactacct caaaaagggt gacgaccacc cttcatacct ccttcactgc caggcgatca
     1741 gaagagtcgt gttcgtcgaa ggccagggcg ttcccgaagc gatcgacttc gatggacagg
     1801 acggctcatc cggccacctc cttctgtttg atacgaacat acccgtcgga acaaccagga
     1861 tccgcaggac cgacaaaggg atgaagctgg aacggatcgc agtcctgccc gcctaccgca
     1921 aaaaaggcta cggcgagctc ctggtgaaag cggccttgag caaagcaacc ggaacgatct
     1981 acatcaacgc ccaggtggca agcctcggct tctatgaaca cctcggcttc gtccgggaag
     2041 acaaaacgac cttccttgaa gccggcatcc cccatacggt catgctctgg ccctacggcc
     2101 aaagtgaagc accttgtcct attttggagc cataataaaa ccccgaagaa aacctccggg
     2161 gtgcgtagag agcaagaaaa cctaccgatt atttctttac attggcatag atcttgttcc
     2221 attgctccaa aacggctttc ttgttcgcga tcaaccagtc gttgtcgcgg gttacccatt
     2281 tgatttccga gcttgccggc aggtacgggg tctcgtagac ggccttttcg ttcgtgaacc
     2341 gaagggtttc aagagccatt ccgagctccg attgtccctc cgcgctcatc aggtaattga
     2401 tcatcgcttt cgcagcgttg gggtttttgg ctcctgcgat caaggcgcaa ccgaatgccg
     2461 aggcggaggc accgtcttcg ggatatacca tcctgatgcg attggaaccc gccttcaaga
     2521 gggtcgaagc gccatcctca taggtcaatc cgacaacata ctcgccggta tcaacgctct
     2581 tgaacgcaac ggagcttgcg gtggagaaca ccacttggtt gttcatgaac ttggtgatgt
     2641 aatcccaaac ctcgggcgtg ccgtggccga agaccgccat catgttgctt acgttgttcc
     2701 aacctgaaga ggatgagttc ggatcgggca tgacgatctt gcccttcagt cgaggatcga
     2761 gcagatcttt atatccgcgg atattgagac caagctcttt ttccagctcc acgttgacgc
     2821 agaataccgc ggtggagagg tggtcaaggc tgtagaaacc gttgttggag cggtagtccg
     2881 gcatgatatc cggctcatat tccgtcagcc aatgctcgaa gagatcgctg tgaaccattc
     2941 cgtcgctgct ggagagtcca ccccacatca agtctccttg agggttgttg cgctctgccc
     3001 ggattctgct ggtgagctcg ccggccgaac cgttgacgat ttcgatcttc acatccggat
     3061 attgtttgtt gaacaagcga accagattgg aaagatcgtt ttccgtcata ctggaataga
     3121 gaacaaggtt cttctccatt gcagtgggat ccgttgcttt ttcttctttt ccgccacctg
     3181 cggcaagcgg caacatcact aaaaagagaa gtgctgccag cacgacgctt attttcgcaa
     3241 tacgtttctt catcgtattc cctccttaaa attatcagcc ttacagaccg atcaggttac
     3301 agtcgaatat cctcggtttt gctgatcact aaatacagga gcagcgaaac cgtcgtaaag
     3361 agcgtcagga tcgcgctgaa agcacaggca agaccatagc ttcccctgga gatcgcgaca
     3421 tacgtggaca tcgtgagcgt gatgttcctg ttgttgtaca ggatgatcga gcttgaaagc
     3481 tcggtgacga tcgccaccca gctgagaatg gcgccgctga tgattccatt gaccatcatc
     3541 ggcacggtta tcaccacgaa ggtcttgagc ttggacgcac cgaggctgat cgcggcctct
     3601 tcgacgctta tcggaatctg catgagaatc gcgttcgtcg atcgtatcga atacggtatg
     3661 cgccggatca cgaacgagat gatcatgatc gccatcgtgc ccgagagaac caacggcggt
     3721 tggccgaatg cgacgaccag agcgatgccg atgatcgctc cgggcatgat gtacggcagc
     3781 atcgacagcg tgtcgatgac gttgtttatc cagctgggcc tgcgcacgac aaggtaagcg
     3841 atgagcactg aaaagacgat gatgatcgcc agagccccga accccaggac aagggtgttc
     3901 ttgatggaac ggaacagaag c
//
LOCUS       DAOPUZ010000051         3914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_23448_length_3914_cov_4.407100, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000051 DAOPUZ010000000
VERSION     DAOPUZ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23448_length_3914_cov_4.407100"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PKN79_01580"
     CDS             complement(<1..754)
                     /locus_tag="PKN79_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNZ94484.1"
                     /translation="MHPKKQLLDDAAKLLLAELEGKELTARERNQIPLQEMATQDPSE
                     RILNMNEVALGYTEAQVRIEAMRCLQCKTKPCVKGCPVGIDIPAFIHEAEKGNYAQAV
                     SIIKESSLLPSVCGRVCPQELQCQMYCTVGKAKKDVEQSVSIGRIERFVADYTREHGL
                     DEIPHVGDNSGKSVAIVGSGPASISAAADLRREGHRVVLFEALHKAGGVLVYGIPEFR
                     LPKEIVEYELEILRKMGVEIRLNYLVGKTRTVD"
     gene            complement(757..1605)
                     /locus_tag="PKN79_01585"
     CDS             complement(757..1605)
                     /locus_tag="PKN79_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNZ94485.1"
                     /translation="MNKIIAKKQLSATVFQMEIEAPEIAEARKAGQFIILQLGGEFDE
                     RIPLTIADADPVKGTITIIFQAVGESTHHIALLKEGDYIENLLGPLGNPTDVRHYGHV
                     VCTGGGIGAAPLFPIVEAMKAAGNRVTVILGARTKELVILEEKMRAVADEVIIVTDDG
                     SYGEKALVTEPLKRICETTQVDCVVAIGPPVMMKFVSLTTKPFGIYTIVSLNTIMIDG
                     TGMCGGCRVTVDGRTRFVCVNGPEFDGHLVDWDNLLLRMGTFKAQEEEAHHRCLIGLD
                     AKEGEA"
     gene            1724..2068
                     /locus_tag="PKN79_01590"
     CDS             1724..2068
                     /locus_tag="PKN79_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNZ94486.1"
                     /translation="MIKRKDEMNTEVRSKMRGGSGEARFTYLMNANEVPHARLFSTIT
                     LDPGSSIGEHTHTNEIEYYWILQGEGVVTEQDGEKVVREGDLVITGGGASHAIRNEGT
                     TPLRFLALIILE"
     gene            complement(2129..2551)
                     /locus_tag="PKN79_01595"
     CDS             complement(2129..2551)
                     /locus_tag="PKN79_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNZ94487.1"
                     /translation="MKYYVTYNRNNPVSSFKSLFNDLWGDWESGSARIPPVDIYETEK
                     SYVIEAELAGYKQDEVKVNVDKHILTISSDKESLKDVDGKKSLVRERIYRKFERSFSL
                     PEGIDENKIEGEFTDGVLTLTLPKKKEVLPKTIEVKIK"
     gene            complement(2796..3914)
                     /locus_tag="PKN79_01600"
     CDS             complement(2796..3914)
                     /locus_tag="PKN79_01600"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HNZ94488.1"
                     /translation="MMEGMNLKYGCNPNQKQAALLMESGTMPIQTLNGEAGYINLLDA
                     LNGWQLVKELKEATGFPSATSFKHVSPAGAAIGLPLNEEEAKVFDAPEELSPIAGAYV
                     RARNADPLASFGDFVAISDECDLSLANVLKREVSDGIIAPSYAPEALELLKTKKRGRY
                     VILQVKAEYEPPLMEKRTLFGMTLMQERNTVQLDSSLLKTIVTKKKELPPEVVRDLLI
                     GLVTLKYTQSNSVCYTKGGQTIGVGAGQQSRIACTRLAGEKADLFHLRFHPKLQDLKP
                     DTSATRQGRHLFIEEGIRNGTLLSKAEQRSCLKGISGVSLTSDAFFPFRDNIDRAAES
                     GVAYIAQSGGSTRDEEVIRAADEHNMVMCMTGIRLFHH"
BASE COUNT          814 a         1117 c         1033 g          950 t
ORIGIN      
        1 catccaccgt gcgggtcttt ccgacaaggt agttcaatcg gatctccacc cccatcttcc
       61 gaagaatctc cagctcgtat tcgacgatct ccttcgggag gcggaactcg ggaatcccgt
      121 agaccagaac gcctcccgct ttgtgcagcg cttcaaacag cacaacccgg tgcccctccc
      181 ggcgtaaatc cgctgcggcg gatatgctgg ccggaccgct tccgacgatc gccaccgact
      241 tgccgctgtt atccccgacg tgagggatct catcaagacc gtgctcccgg gtgtaatcgg
      301 cgacgaaacg ctcgatccgc ccgatcgaaa cggattgctc cacatctttt tttgccttcc
      361 ccacagtgca gtacatctgg cactggagct cctggggaca gacccgtccg cagacggagg
      421 gaagcaggct tgattccttg atgattgaaa ccgcctgtgc atagtttccc ttctccgctt
      481 catggatgaa ggcgggaata tcgatgccga cggggcatcc cttgacgcag ggcttggttt
      541 tacactggag gcagcgcatc gcttcgatgc ggacttgggc ttcggtatac ccgagggcga
      601 cctcgttcat attgaggatt cgttcgctcg ggtcctgagt ggccatctcc tgcaggggaa
      661 tctgattgcg ctctctggcc gtgagctctt ttccctcaag ctcggcgagc agcagtttcg
      721 cggcgtcatc aagtagttgt ttcttcgggt gcatctctac gcctctcctt ctttggcatc
      781 caagccgatg agacagcggt ggtgcgcctc ttcttcctgg gccttgaagg tgcccatccg
      841 aagcagaagg ttgtcccaat cgacaaggtg tccgtcaaac tcaggaccgt tcacgcacac
      901 gaagcgggtg cgcccgtcga ccgttacgcg gcatcctccg cacattcccg tgccgtcgat
      961 catgatcgtg ttcaaactga cgatcgtata gattccaaag ggcttggtgg tcagcgagac
     1021 gaacttcatc atcaccggag gtccgattgc cacgacgcaa tcgacttggg tcgtttcgca
     1081 gattcgcttc agcggctcgg ttaccagcgc cttctctccg tatgaaccgt catcggttac
     1141 gatgatcact tcatcggcga cggcgcgcat cttctcttcc agaatcacca actctttcgt
     1201 ccgggctccg agaatcaccg ttaccctgtt gcccgccgct ttcatcgcct cgacgatggg
     1261 aaagagcgga gccgctccaa ttcctccgcc ggtgcacacc acatggccgt agtggcggac
     1321 atcggtgggg ttgccgagcg gtccgaggag gttttcgatg taatcgccct cctttaggag
     1381 cgcgatatgg tgggtcgatt cgccgaccgc ctgaaagatg atggtgattg tccccttgac
     1441 gggatcggca tcggcgatcg taagagggat ccgttcatca aactctccgc cgagctggag
     1501 gatgataaac tggccggcct ttcgcgcttc agcgatttca ggagcttcaa tctccatctg
     1561 aaacactgta gcagacaact gttttttcgc aatgattttg ttcacacccg cttcctccga
     1621 tacctcattc gtcccatgaa aacgatacaa tagtattgaa agtggcagca ttgttgtcaa
     1681 gtcatccctt ggcaaacccc cgttgcggtg gcacactgtt catatgatta aacgaaaaga
     1741 tgaaatgaac accgaagttc ggtccaagat gagaggggga agcggcgaag cccgcttcac
     1801 ctacctgatg aatgcaaacg aggtcccaca cgcccggttg ttttcgacga tcacgctgga
     1861 tcccggctcc tcaatcggcg agcacaccca cacgaacgag atcgagtact actggattct
     1921 ccaaggcgaa ggtgtcgtca ccgagcagga cggagaaaag gtcgtcaggg aaggtgatct
     1981 ggtgattacc ggcggtggag cgagtcatgc gatccgcaat gaaggaacaa cgccgctgcg
     2041 cttcctcgcc ttgatcatcc tcgagtaggg agaaatggtt tcaggcagcc tcccggctgc
     2101 ctgaatgtac aaagctgatt cgtttttctt acttgatctt cacttcgatc gtcttgggaa
     2161 ggacttcctt cttcttggga agggtgagcg tcaagacacc gtcggtgaac tcgccttcga
     2221 tcttgttctc atcgattccc tcgggcaggc tgaaggaacg ctcgaacttc cgatagatcc
     2281 gctcccggac aaggctcttc tttccatcga cgtccttgag gctctccttg tcgctggaga
     2341 ttgtcagaat gtgcttgtca acgttgactt tgacttcatc ctgcttgtag ccggcaagct
     2401 ccgcttcgat gacataactc ttctcggtct catagatatc caccggagga atccgggcac
     2461 tgccggactc ccagtcgccc caaaggtcgt tgaacaggga cttgaagctc gataccgggt
     2521 tgtttcgatt ataggttacg tagtatttca taaggcacct ccactgtgca ttgttgttct
     2581 actcaacatc atgcaaatgg cgtgccaacc tcggcgaata aatcgttatg ttatatttaa
     2641 actattacta tataatgatt tacattgttt tttcgaaaaa agagatatga aacaaacagg
     2701 aaaccatcct cgaagaagaa agaaatacgg catatttgaa tcaaaatgac acacttgggc
     2761 tataaagacc cttttttggt tcttcatgac ccaactcaat gatgaaacag ccgaatcccc
     2821 gtcatgcaca tcaccatgtt gtgctcatcc gccgcccgga tgacctcctc gtcccgggtg
     2881 gaaccgccac tctgggcgat gtacgcaaca ccgctttcgg ccgctcggtc gatgttgtct
     2941 ctgaagggaa agaacgcgtc gctggtcagg cttaccccgg aaatgccctt cagacaggat
     3001 cgctgctccg cttttgaaag cagcgtcccg tttcgaattc cctcttcgat gaagagatgc
     3061 cggccttgcc tggtggcgga cgtgtccggc ttgagatcct gcagcttggg gtgaaagcgg
     3121 agatggaaga gatccgcttt ttcccctgca agacgggtgc aggcaattcg cgattgctgt
     3181 cccgctccca ctccgatcgt ctggccccct ttggtgtagc acaccgagtt ggattgggta
     3241 tatttgagag taaccagacc gatgagaaga tcccggacca cttcgggggg aagctctttc
     3301 ttcttcgtga cgatcgtctt caggagcgag gagtcgagtt ggacggtatt ccgctcctgc
     3361 atcagggtca ttccgaacaa ggttcgtttt tccatcaggg gcggttcata ctcggccttc
     3421 acctgaagga tgacgtagcg cccccgtttc tttgttttca aaagttcgag cgcttccggg
     3481 gcataggaag gagcgatgat tccgtcgctc acctcccgtt tgagcacatt ggccagggag
     3541 aggtcacact cgtcgctgat cgcaacgaaa tcgccgaagg aagcaagcgg atcggcgttt
     3601 cgcgctcgga catacgcgcc ggctatcggg gaaagctcct ccggtgcatc gaagactttc
     3661 gcctcctctt cattcagggg aagcccgatc gccgctcccg ccggcgagac gtgtttgaac
     3721 gaggtcgcgc tcggaaaccc tgtcgcctct ttgagctctt tgaccagctg ccacccgttg
     3781 agcgcatcaa ggaggttgat gtatcccgct tcgccgttga gcgtttgaat cggcatcgtc
     3841 ccgctttcca tcaagagggc cgcttgcttc tgattggggt tgcagccata tttcagattc
     3901 attccttcca tcat
//
LOCUS       DAOPUZ010000052         3901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_23560_length_3901_cov_2.762871, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000052 DAOPUZ010000000
VERSION     DAOPUZ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23560_length_3901_cov_2.762871"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..680
                     /locus_tag="PKN79_01605"
     CDS             66..680
                     /locus_tag="PKN79_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNZ94489.1"
                     /translation="MNNNSIVSKNTKVFLFDMGNVVVKGIGISEKIAERLDVPHDQFE
                     LDYHHWIYPLMEGEVSAEQYWRHAEKVFGKRIEEDLMATCFTPYLNSEVVETITRLRN
                     LGYRVVCASNTYASHWEILRKMDALALFDALYPSHEVGLSKPAHAYFRHILEAEGVKA
                     SEAYFVDDSIEHIESARTMGIPSLHYSGKAASRELKRVFEPLLG"
     gene            complement(669..1439)
                     /locus_tag="PKN79_01610"
     CDS             complement(669..1439)
                     /locus_tag="PKN79_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ94490.1"
                     /translation="MAYTVVFVEDEELVRREIVSSIRWEALGLKLVGVAADGAEGERL
                     IKELEPDIVITDIRLPAQDGLSMLASCPVDHAIILTGHTDFTYMKQAIRLGVFDYLLK
                     PVSDEELEEALVSLVQKIQEEDRDVEQINRRHREHDHSITLPKHFKNHVIDSAVAFIV
                     DNYSQSVGLQEAANHLGVSESHLSRLFKEVTGLNFLQYLNAYRINKSVEAMSDPKKNI
                     AEIATSCGFPTPGYYAKIFKRFFGRTPTQFRDILGSPQ"
     gene            complement(1443..3188)
                     /locus_tag="PKN79_01615"
     CDS             complement(1443..3188)
                     /locus_tag="PKN79_01615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNZ94491.1"
                     /translation="MADRSRTLFVRLLIYFLIVLLLPLLVFSGYYLFLGDRSQSKYLS
                     EQSLGVIRRDALIVGNVLEEYRHKAYLLSTDELIVEVMKHDSPEFLNRVIHEVYPLLF
                     SVMKGDTYLASANLVSNTGRIRLSTHAFPKMFDLRYHTSTSELNSVIAQNLNLSQTAS
                     LITTRGHRTAENGRQVVASILRRIYDGEGTNLGYLVIDLFGEALSTGINSERLFSDLY
                     LIDQKEFHATSLIHTDMHGSFDKFPPLLSLKGDYTPRSVQVGSTVVAVAPVANTTNLY
                     LAGTLSTVFFSRNISRWYLVFTGTLIAGFALAMMFSFLFSRSIAHPIASLARRMDRVD
                     RMNLETRPVKSTITEFAQLENSYNMMIDQIRTLLEQTREEQKKLSEAERKALESQMNP
                     HFLFNTLNTIKALARINNQEEIYTITVKLGKLLRSTIDSHESEATLAHSIALIDSYLT
                     IQKLRYGEKLVTSIHLDPACAHIKTPKLIIQPLVENAIIHGLEPKVGNWFLAVRVEAV
                     GRHIQITVEDNGVGFDRATLPQDLNDLAHSTHVGLYNVYRRLSLAYQNELEFSIDSTV
                     GKGTIVRISLPIGRR"
     gene            3547..>3901
                     /gene="ilvN"
                     /locus_tag="PKN79_01620"
     CDS             3547..>3901
                     /gene="ilvN"
                     /locus_tag="PKN79_01620"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNZ94492.1"
                     /translation="MSKREKRYTLAILVNNNPGVLMRVVSLFSRRGYNIDSLSVGETE
                     DERFSRITIVVPGDRAVVEQIKKQVAKLYDVIQVYEMTRTASLQRELIMIKVNASVAS
                     RTEVVELAQIFKAKII"
BASE COUNT          903 a          975 c         1119 g          904 t
ORIGIN      
        1 catgagcctc acgaaatact tcatcggcgg atcggcggtc tacgaatcac gcaagggtta
       61 agcgagtgaa caacaattcc atcgtcagca aaaacacgaa ggtgtttctc ttcgacatgg
      121 gcaacgtcgt cgtcaaaggg ataggaatca gtgaaaagat cgccgaacgc ctggacgttc
      181 cccacgatca gttcgagttg gattaccacc attggatcta tcccttgatg gaaggggagg
      241 tcagtgccga gcagtattgg cggcacgctg aaaaggtgtt cgggaaacgg atcgaagagg
      301 atttgatggc tacctgcttc accccgtacc tgaacagcga ggtggtcgaa acgatcacaa
      361 ggctgcggaa tctgggatac cgggtggtgt gcgcctccaa tacctacgcg tctcattggg
      421 agatccttcg aaagatggat gcgctcgccc tctttgacgc gctctatccc tcccacgaag
      481 tagggctttc caaaccggct cacgcatact tccggcatat tctcgaagct gaaggagtca
      541 aggcgagtga agcatatttc gtcgacgatt cgatcgagca catcgaaagc gcccggacta
      601 tggggattcc ttctctgcat tacagcggca aagccgcttc aagagagttg aagcgagtgt
      661 ttgaacctct attggggtga tccaaggatg tcgcggaact gggtcggagt cctgccgaaa
      721 aagcgtttga agatcttggc atagtacccg ggggtgggaa agccgcaact ggttgcaatt
      781 tcggcgatgt tctttttggg atcgctcatc gcttcgacgc tcttgttgat ccgatacgcg
      841 ttgagatact ggaggaagtt gagccccgta acctctttga agagtcggga gaggtggctc
      901 tcactgactc ccagatgatt ggccgcttcc tgcaacccga ccgattggct gtagttgtcg
      961 acgatgaagg cgaccgccga atcgatcaca tggttcttga agtgcttggg aagggtaatg
     1021 gaatgatcgt gctcccggtg gcgccggttg atctgctcca catcccggtc ttcctcctga
     1081 atcttctgaa ccagggaaac aagtgcctct tcaagctcct catcgcttac cggtttcaaa
     1141 agatagtcaa acacaccgag gcggatcgcc tgtttcatgt aggtgaagtc ggtgtgaccg
     1201 gttaagatga tcgcatggtc caccgggcac gaggcgagca tcgaaagtcc gtcctgagcg
     1261 gggaggcgga tatcggtaat cacgatatcc ggctccaatt ccttgatgag tcgttctcct
     1321 tccgccccgt cggcggcgac tcccaccaat ttcaaaccca aggcttccca ccggatcgac
     1381 gagacgatct cccggcgaac aagctcttcg tcctctacga acacgacggt gtatgccacg
     1441 acttatctcc ttcctatggg caggctgatg cgaacgatcg ttcctttccc taccgttgaa
     1501 tcgatcgaaa actcaagctc gttttggtat gcgagtgaaa gtcttcgata tacattgtat
     1561 agcccaacgt gggttgaatg tgcaagatcg ttgagatcct gcggcaacgt cgcacggtcg
     1621 aacccgactc cgttgtcctc gaccgtgatc tgaatgtgcc gtccgaccgc ttcgacccga
     1681 accgccagaa accaattgcc tactttaggc tccagaccgt ggatgattgc attttcaacc
     1741 agcggttgga tgatgagctt tggggttttg atgtgggcgc atgccgggtc gaggtggatc
     1801 gaggtgacga gcttttcccc gtaacggagc ttttgaatcg tcaggtagga atcgatcagg
     1861 gcgatcgaat gggcgagggt cgcttcgctt tcatggctgt cgatcgtcga gcggagcagt
     1921 ttccctagtt tcacggtgat cgtgtagatc tcttcttggt tgttgatccg ggccagagcc
     1981 ttgatcgtat taagagtgtt gaagaggaag tggggattca tctggctttc cagcgcttta
     2041 cgctccgctt cgctgagctt cttctgctcc tcccgggtct gttccagaag cgttctgatc
     2101 tgatcgatca tcatgttgta ggagttttca agctgagcga attcggtgat cgtcgatttg
     2161 accggtctgg tttccaggtt cattcggtcg acccgatcca tccgtcgggc cagggaagcg
     2221 atcggatggg cgattgaacg ggagaagagg aagctgaaca tcatcgccaa ggcgaaaccg
     2281 gcgatgagcg tcccggtgaa gaccagatac caccttgaga tgttcctgct gaaaaacacc
     2341 gtgctgagcg ttccggcaag atagaggttg gtggtattgg ccaccggagc cacggcgacc
     2401 accgtcgaac cgacctggac ggatcggggg gtgtaatctc ctttcaggga aagcaacggg
     2461 gggaacttgt cgaaagaacc gtgcatatcg gtatggatca ggctggtggc gtggaactcc
     2521 ttctgatcga tcagatacag atcgctgaac agccgttcgc tgttgattcc ggtgctgagc
     2581 gcctcgccga agaggtcgat caccagatac ccgagattgg ttccctcccc gtcatagatt
     2641 cttctcagga ttgaagcaac cacctgccgg ccgttttcag cggtccgatg cccccgggta
     2701 gtgatcaagc tggcggtttg cgagaggttg aggttttggg cgatgacgct gttcaattca
     2761 ctggtggagg tgtggtaccg caaatcgaac atcttgggaa aggcgtgggt cgaaagccgg
     2821 attcgccccg tattggaaac gagattcgcg ctggcgaggt aggtgtctcc cttcatcacg
     2881 ctgaagagaa ggggatagac ctcatggatg acccggttga ggaactccgg cgagtcgtgt
     2941 ttcattacct cgacgatcag ttcatcggtg gagagaagat atgctttatg gcggtattcc
     3001 tccagaacat tgccgacgat gagggcgtcg cggcggatca cgccgaggct ttgctcggaa
     3061 aggtacttgc tttgacttct gtccccaagg aagagatagt atccgctgaa aaccaataac
     3121 ggaagcaaca atacgatgag aaagtagatt aataagcgta caaagagggt acgactccga
     3181 tccgccatag acaaagtgta ccgacaaact tctcctcgaa caataggaag gaatgttcgt
     3241 atattgcgtg tataaagatg ctatttttga ataatgggtt gacagagaag gcggaagaga
     3301 ggtacaaaca ctcaatacct gcaggtgcag gtgcaaggag tggagtgtga acggcacctt
     3361 gaagatccag gttacccgaa cgaacgagct tgccatgacg agctcgaacc ttttcatctc
     3421 cgccctcatt attctagtac ttattattct ctgaccacgg ctgcagaata tctgcatcgt
     3481 ccgtggaagc ccccctgcca agggaggtct tttttgtatc caacgtgttg aggaggaggg
     3541 aacacgatga gtaaacgaga aaaacgctac accctggcga tcttggtgaa caacaatccc
     3601 ggagtgctga tgcgggtcgt ctcgctcttc agtcgaaggg gatacaacat cgacagcctc
     3661 tccgtcggcg agacggagga tgaacgcttc agccggatca cgatcgtcgt ccccggtgat
     3721 cgagcggttg tcgagcagat caagaagcag gtcgccaagc tgtatgatgt gattcaagtc
     3781 tatgagatga cccgcaccgc cagtctgcag cgcgagctga tcatgatcaa agtcaatgct
     3841 tcggtggcga gccggaccga agtcgtcgaa ctggcccaga tcttcaaagc gaagatcatc
     3901 g
//
LOCUS       DAOPUZ010000053         3870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_23805_length_3870_cov_2.310354, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000053 DAOPUZ010000000
VERSION     DAOPUZ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23805_length_3870_cov_2.310354"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKN79_01625"
     CDS             <1..360
                     /locus_tag="PKN79_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ94493.1"
                     /translation="RISGGELQRTFLARALAQDGKILLLDEPVNHLDIAHQRSVMHLL
                     QGLVAKGYTVISVLHDLLLAQAYSSWIIFLKEGAVAADGPTDEVVNEEILKSVYNVKS
                     HRIYDPHLDKELLIPAW"
     gene            443..1411
                     /locus_tag="PKN79_01630"
     CDS             443..1411
                     /locus_tag="PKN79_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ94494.1"
                     /translation="MNLAILGAGNIGNKMATTVKQMEGVTLHAVASRSLAKAKEFAKT
                     YSIPKAYGSYEEMLKDRDIELVYVCTPHSHHYEHMKLALEHGKHVLCEKAFTVNAKQA
                     KEILDLGKQKGLLVAEAIWTRYLPMRTVLDQILARRVIGEVQTLTANLAYPVSRIERM
                     WNPELAAGTLLDLSVYTINFAMMVFGSRIETIDGVCTRTNTGVDESNSITLTWAGGKM
                     ATLHASMLGIGDRRGALFGTKGYIEFENINNCEGIQVYDKEYNLIETYKPFKQITGFE
                     FQVEACKRAIEAGKTECEQMPHSEILAVMNVMDELRRQWNLVYPME"
     gene            1411..1908
                     /locus_tag="PKN79_01635"
     CDS             1411..1908
                     /locus_tag="PKN79_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNZ94495.1"
                     /translation="MMELQELIALVDRQEELLRLKTFNSSIAWKLGNLFHEKIVKEDL
                     PIAVSIRSINGKTLFHYSSDKANYGSQGWLDRKFNTVRHFETSTLRYALFLRSRGATL
                     LERGLDPSKFVSGGGGFPIRTIDSTLVGAVLVSGMKDTEDHAIIIEVLSQYLDVINVP
                     IYPIS"
     gene            complement(1893..2441)
                     /gene="cyaB"
                     /locus_tag="PKN79_01640"
     CDS             complement(1893..2441)
                     /gene="cyaB"
                     /locus_tag="PKN79_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNZ94496.1"
                     /translation="MAFEVELKAWVRKAEALKRSLDTKEGLLYVGFEEKEDTYYALEE
                     LPLLRIRKEVVNEERSLVVTHKKRSTIDGIEENLEYEFSVDPRQEQAVHAFFTALGYR
                     VAKRKFKRGWRYRQVHTTTTPGLTVELVHLEGLGWFIEVEAMVETQDERRQAREELMA
                     FLASLGIDSSAIESKPYLQLIG"
     gene            complement(2443..3162)
                     /locus_tag="PKN79_01645"
     CDS             complement(2443..3162)
                     /locus_tag="PKN79_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94497.1"
                     /translation="MVVHNLGYLLLLFAFFGVLSLVSTSTLLFFIVIILGTGALAFYT
                     AGASSASWAYARYQRPGWSDFKRGIRISWRHALLFWFLLLLVLTLVFLVIPFYLSFEN
                     IVGIILGVIIFWVTLLLIFALMYFFPLFFAMQGDRPMKTLKKSFLLVLDNPFFTLFLA
                     LYQVINTLFSLLLAFLAPGFTGISLAGSVATKLLLFKYDYLEEHPETGRKAIPWDDLL
                     YEEREAVGPRSLKGMIFPWKD"
     gene            complement(3219..>3870)
                     /locus_tag="PKN79_01650"
     CDS             complement(3219..>3870)
                     /locus_tag="PKN79_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ94498.1"
                     /translation="DGPTAIFVTTKLWNGVRGYKETLQAAKESMEKLQVEYLDLYLIH
                     WPNPAAHRHWWKEANAESWKAMEELYENGAIKAIGISNFEERHIEALLASAQVIPHTN
                     QIRLFAGEQQRALVAYCRNKGMITEAYSPLGVGGILSNEVIGRIAKETKRTPAQVALR
                     YNLQKDHVVLPKSVHRDRMVENLNLFDFELSREQMISLDALPNTISATRNPDEVPF"
BASE COUNT          957 a         1029 c         1031 g          853 t
ORIGIN      
        1 cgaatctccg gcggggagct gcagcggacc tttctggccc gggcgctcgc tcaggacgga
       61 aagattcttc ttctcgatga gccggtaaac cacctggata tcgcccacca acgaagtgtg
      121 atgcacctgc ttcaaggcct cgtagcaaaa ggatataccg tcatctccgt tcttcatgac
      181 ctgcttctgg ctcaagccta cagctcctgg atcatcttcc tcaaggaagg ggcggtcgcc
      241 gcggacggtc cgaccgatga agtggtgaac gaagaaatcc tgaaatccgt ctacaacgtt
      301 aaaagtcacc gcatctacga tcctcatctg gacaaggagc ttttgatacc cgcctggtag
      361 atttttagtg aaacaaggtg ttcgcatctc cttttcttca attcttgcga tatactcata
      421 ggtatcacaa ggaggatacg acatgaacct ggcgattctc ggtgccggca acatcggcaa
      481 taagatggca acgacggtga agcagatgga aggagtgacg ctccatgcgg tcgcttcccg
      541 atcattggcc aaagccaagg agtttgccaa aacatactca atccccaagg cgtacgggtc
      601 ctatgaagag atgctcaagg atcgtgatat cgagctggta tatgtgtgca ctccccactc
      661 ccatcactac gaacacatga aactggcttt ggaacatggc aaacatgtgc tttgtgagaa
      721 agccttcacc gtaaacgcaa aacaagccaa agagatcctt gatcttggaa aacaaaaagg
      781 tctgctcgtc gccgaagcga tttggaccag gtacctgccg atgcggacgg ttttggatca
      841 gatcctcgcc cgacgggtga tcggggaggt ccaaaccctt accgccaacc tcgcttatcc
      901 ggtcagtcgg atcgaacgga tgtggaaccc cgagcttgcc gccggaaccc tgctcgatct
      961 gtcggtctat acgatcaact ttgcgatgat ggtcttcgga agcaggatcg agacgatcga
     1021 cggagtgtgc acgcggacga acaccggtgt ggatgaatca aattccatca ccctgacctg
     1081 ggcgggcggc aagatggcca cccttcacgc ctcgatgctt ggcatcgggg atcgcagagg
     1141 agcgctgttc ggcacaaaag gatacatcga attcgaaaac atcaacaact gtgaaggaat
     1201 ccaagtctac gacaaagagt ataatctgat tgaaacctac aaacccttca agcagatcac
     1261 cggcttcgag ttccaagtcg aagcgtgcaa acgggcgatc gaagcgggaa aaaccgaatg
     1321 cgagcagatg ccgcacagtg agatccttgc cgtgatgaac gtgatggatg agctcagacg
     1381 ccaatggaac ttggtgtatc cgatggagtg atgatggaac tgcaagaatt aattgctctt
     1441 gtcgatcgac aggaagagct gctccggttg aaaacgttca acagttcgat tgcgtggaaa
     1501 ctcgggaatt tgtttcatga gaagatcgtg aaagaagatc ttccaatcgc cgtgtcgatt
     1561 cgctcgatca acggaaaaac ccttttccac tacagcagtg ataaggcgaa ttatggctcc
     1621 cagggatggt tggaccgaaa gttcaatacg gtccggcact tcgaaacatc caccctgcgg
     1681 tacgcccttt ttctccgaag ccgaggcgcc accctgctcg aacggggatt ggatccgtca
     1741 aagttcgttt ccggtggcgg cggatttccg attcgtacca ttgattcgac gttggtcggg
     1801 gccgttctgg tctccggtat gaaggacacg gaggatcatg cgatcatcat tgaggtcctc
     1861 agtcagtatc tggatgtcat aaacgtccct atctacccga tcagctgaag gtagggcttg
     1921 gactcgatcg ccgaggaatc gattcccagt gaggcaagaa acgccatcag ctcctctctg
     1981 gcctgccggc gttcgtcctg tgtctccacc atcgcttcca cctcgataaa ccacccgagc
     2041 ccttcgaggt ggacgagttc caccgtcaat ccgggggtgg tggtcgtgtg gacttgccgg
     2101 tagcgccatc cccgtttgaa ttttctcttc gctacgcgat agccgagcgc ggtaaagaag
     2161 gcgtgaaccg cctgttcctg ccggggatcg acggaaaatt catattcaag attctcttcg
     2221 attccatcga tggtggagcg cttcttgtga gtgaccacca aagaccgctc ctcattgacc
     2281 acctcttttc gaatccgaag gagcgggagc tcttccaagg cataatatgt atcctctttc
     2341 tcttcaaagc cgacatagag aaggccctct ttcgtatcga ggctcctctt caacgcttcg
     2401 gccttgcgaa cccacgcctt gagctccact tcaaaagcca tctcaatcct tccacgggaa
     2461 gatcattccc ttcagcgatc tgggaccgac cgcttcccgt tcctcataga gcagatcatc
     2521 ccagggaatc gctttgcgac cggtttcggg gtgctcttcc aaataatcgt atttgaagag
     2581 gagcagcttc gtggcaacgc tgccggcaag gctgatgccg gtgaacccgg gagccaaaaa
     2641 agccaacaga agcgaaaaga gggtgtttat cacttggtac agtgctagga agagggtaaa
     2701 gaaggggttg tccaagacaa gcaggaagga ttttttgagc gtcttcatcg gacggtcgcc
     2761 ctgcatcgca aagaacaagg ggaagaagta catcagagcg aaaatgagca gcagcgtcac
     2821 ccaaaagatg atgaccccca ggatgattcc cacaatgttc tcgaagctca ggtaaaacgg
     2881 gatcaccaga aagaccaagg tgagaacaag cagcagcaga aaccaaaaga gcagagcgtg
     2941 acgccatgag atccgaatcc cccgcttaaa atcgctccac cccggtcgtt ggtagcgggc
     3001 gtacgcccag gaagcggaag aagctccggc ggtatagaac gcaagcgctc ccgtgccgag
     3061 gatgatgacg ataaagaaca gcagagtgga tgtcgaaacg agtgaaagca cgccaaagaa
     3121 cgcgaagaga agcagaaggt aacccaagtt atggactacc atcgaaatca aattgtccca
     3181 tccgtcgaag aacgcctttt ttatgaaaaa cccaaccatc agaagggaac ctcgtccgga
     3241 ttgcgcgttg cgctgatcgt atttggaagg gcatccagac tgatcatctg ctcccggctc
     3301 agctcgaaat cgaagaggtt gaggttttca accatccggt cgcggtgtac gctctttgga
     3361 agcacgacgt gatccttttg aagattatag cgcagcgcca cttgagcggg cgtgcgcttc
     3421 gtctccttcg ctattcgtcc gatcacctcg ttggagagga ttccacctac cccgaggggg
     3481 ctgtatgctt cggtgatcat ccctttattt cggcagtagg cgaccaatgc gcgctgctgt
     3541 tctccggcaa agagccggat ctggttcgta tggggaataa cctgagcgct tgcaagaagc
     3601 gcttcgatat ggcgctcctc gaagttgctg atcccgatcg ctttgatcgc tccgttttca
     3661 tagagctcct ccatcgcctt ccagctttcg gcgttggcct ctttccacca gtgtcggtgg
     3721 gcggcgggat tgggccagtg gatgaggtac agatcgaggt attcaacctg gagtttttcc
     3781 atcgactcct ttgccgcttg aagcgtttct ttataccctc gaacaccatt ccacagcttg
     3841 gtggtaacaa agatggcggt cgggccgtca
//
LOCUS       DAOPUZ010000054         3847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_24001_length_3847_cov_3.264504, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000054 DAOPUZ010000000
VERSION     DAOPUZ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24001_length_3847_cov_3.264504"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PKN79_01655"
     CDS             <1..732
                     /locus_tag="PKN79_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HNZ94499.1"
                     /translation="LTLFGMAGSIIVSLAGSRLFLPKDLDTTIAVARRKEGSLLRYVW
                     DLLKEPLFLISIVILGVIFGMKSTFFDWFKSSYVSYLLYVMLFFVGMNLIQRSVSFKT
                     VLRDPSVFLLPVYTIAGTLLGAFGASLATSFSFREAAGMLSGFGWYSLSGILISDLGF
                     PLLGSISFLSNLMRESFSFFLIPLFGRLGKRYYYPAVSCGGATTMDVLLVLLSKHFGS
                     ATTIGSIYHGAATSLCAPVLIPLFF"
     gene            complement(713..1138)
                     /locus_tag="PKN79_01660"
     CDS             complement(713..1138)
                     /locus_tag="PKN79_01660"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94500.1"
                     /translation="MSMMNLSLRQSLAYQKLPSEGSSAEDAYRALISFLDQAPAGSEG
                     ILLLSFEMNVLFLGMSDPPNEETLEKIARAEKLDPAEGDHVLEPGHYRFIQIPLPASI
                     EELPLENLALDEGDLLYLRILKEGSFALVAQLWIRRRAE"
     gene            1157..1786
                     /locus_tag="PKN79_01665"
     CDS             1157..1786
                     /locus_tag="PKN79_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNZ94501.1"
                     /translation="MKSTGKVGTIRYTLLISRANRRITIRVGSDGILAIRAPRRVSRR
                     VIEELITEHKEHLLNRIDEEVRDAHTYEDGDRFPYAGGMVALQLRTGNDYRAYIEDDL
                     LVVSARNIQDKGRILHAIKTLYRAEARRRIGHLVEQWSRTLGVSAPSFTIRDSKKRWG
                     SCSKEGRLNFSLRSQALGEKELSYLVLHEVAHLVHFNHSKEFKALLQPT"
     gene            1905..3119
                     /locus_tag="PKN79_01670"
     CDS             1905..3119
                     /locus_tag="PKN79_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNZ94502.1"
                     /translation="MKPDLLTDHVYRVAAKIGNRDLFEGIWPIPDGVMLNSYVVKGSK
                     ATALIDVVKDWDGALDAINRQLDELSITTNTLDFLIINHMEPDHTGALREVVKRYPDV
                     QILCSPKAVPLIKAFYKIEENVQAVADGQTLDLGGKTLVFHLTPNIHWPETMMTYLQE
                     DEILFSCDAFGSFGRYDFCFDDELDEATMAHLHSETERYYANIVSSFSPFVLRGIEKL
                     KGLKIAMVAPSHGVVWRSHPEAIINHYIKLASYLNGPREKEVALVWSSMYGNTQALLE
                     SIIAGVESEGLPIHVLQVPQTHESFVLEKVWRSEGLIVGMPTYEYKMFPPAYHVLDIL
                     ARSHVKGRKTLRFGSYGWSGGAQKQYDEFVTAMKLECVAEAIEFQGSPTQEDKDKAFA
                     RAKELARIIKAM"
     gene            complement(3123..>3847)
                     /locus_tag="PKN79_01675"
     CDS             complement(3123..>3847)
                     /locus_tag="PKN79_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNZ94503.1"
                     /translation="LDEYRVFKATGGSKRNVIPSTATIGFSIPKEDEDRLYQVVETVL
                     KEVKGEFEVADPHIAIDVTKVETPKRSVVANTLFDALYAAPHGVDAMSMSIKGIVETS
                     TNLAILALDEKGFYVSASHRSSILSARDDVARRFATIFKLAGATTKHSGAYPSWTPNI
                     HSPLAALVARAWKEYTNEDAKVTAIHAGLECGIINSRISGMDSVSFGPNMEEVHSTKE
                     RVSISSTGRISEFTRYLLETIR"
BASE COUNT          857 a         1046 c         1053 g          891 t
ORIGIN      
        1 ctgaccctct tcggtatggc cggctcgatc atcgtttccc ttgccgggtc ccgcctgttt
       61 ctgcccaagg atcttgatac aacgatcgcc gtcgcccggc gaaaggaagg ctctctgctt
      121 cgctatgtgt gggacctttt gaaagaaccg ctgtttctga tttcaatcgt tatcctcgga
      181 gtgatttttg gaatgaaaag caccttcttt gattggttca aatcctccta tgtgtcgtat
      241 ctgctctatg tgatgctctt tttcgtgggg atgaacctga ttcagcgttc cgtctccttc
      301 aaaacggtgc tccgcgaccc gagcgtcttt ctcttgccgg tctacacgat cgcaggaacc
      361 cttctggggg cgttcggagc gagccttgcg acctccttct cctttcggga agccgccgga
      421 atgctcagcg ggttcggatg gtattcgctc tcgggaatcc tcatcagtga tctgggattt
      481 cctcttttgg gctcaatatc ttttctctcc aacctgatgc gcgaaagctt ctcgttcttc
      541 ctcatccccc tcttcggacg attgggaaaa cggtactact atccggcggt ctcctgcgga
      601 ggggccacga cgatggatgt gctgctcgtg ctgctttcca agcatttcgg ttcggcgacc
      661 acgattggat cgatctatca tggggcggct acgagcctct gcgccccggt tctcattccg
      721 ctcttcttct gatccacagc tgagcgacca aggcaaagct tccttccttc aggattcgaa
      781 gatagagcag atcaccctcg tccaaagcga ggttctccaa ggggagctct tcgatcgaag
      841 caggtagggg gatctgaatg aagcggtagt gtcccggttc aagaacatga tcgccctccg
      901 ccggatcaag cttctccgct ctggcgatct tttccagcgt ttcctcattc gggggatcgc
      961 tcattccaag gaagaggaca ttcatctcaa aggagaggag aaggatccct tcgcttcctg
     1021 cgggtgcctg atcaaggaac gagatcaatg cccgatatgc atcctcggcg gaacttccct
     1081 cagaggggag tttttggtac gctaggcttt ggcggaggga gaggttcatc atgctcattg
     1141 aggctactct aacaaaatga aaagcaccgg taaagtagga acgatccggt acacgctgct
     1201 catctcccgg gcgaaccgcc ggatcacgat ccgggtcggc tcggatggaa tcctggcaat
     1261 ccgggctccg agacgggtaa gcaggcgggt tattgaagag ctgatcaccg aacacaaaga
     1321 gcatctcctc aatcgaatcg atgaagaagt gcgggacgct catacctatg aggacgggga
     1381 tcggttcccc tatgcgggag gaatggtcgc tctccaactt cgaacaggaa atgattatcg
     1441 ggcatacata gaagatgatc ttctcgtcgt ttccgcccga aacatccaag acaaagggcg
     1501 gattcttcac gcaatcaaaa cactttatcg agcggaggca agaagacgga tcgggcacct
     1561 tgtggagcag tggagccgga ccctcggagt atcagctcct tcgttcacga tccgtgattc
     1621 aaaaaagcgc tggggaagct gctccaaaga ggggcggttg aatttctctc tccgttctca
     1681 ggcgctcggc gagaaggagc tttcctatct tgttcttcat gaagtggcac acttggtcca
     1741 cttcaaccac tccaaagagt tcaaagcact ccttcaaccc acatgagtga ttataagatc
     1801 cggcaaaaac gaatgtacgc catgcaacgg gcgagtcagc tgattcactg atatacatcc
     1861 accaactctt taaggtatag tcccatagca ggaggattgt tgagatgaaa cccgatctgt
     1921 taactgatca tgtatatcgc gtcgcagcga aaatcggaaa ccgtgattta tttgaaggca
     1981 tttggccgat ccccgacggt gtgatgctca actcgtatgt cgtgaagggc agcaaagcca
     2041 ctgcgctcat cgacgtggtc aaggattggg acggggcgct tgatgccatc aacaggcagc
     2101 ttgacgagct ttccataacg accaacacgc tggacttttt gattatcaac catatggaac
     2161 cggatcatac cggcgccctg agggaggtgg tgaagcgcta tcccgatgtc caaatccttt
     2221 gttcgccgaa ggcggttccc ctcatcaaag cgttctacaa gatcgaagag aacgttcagg
     2281 cggttgccga cggacagacc ctcgacctcg gaggaaagac gcttgtcttc caccttaccc
     2341 cgaacatcca ctggccggag acgatgatga cctaccttca ggaggatgaa atccttttct
     2401 cgtgcgatgc attcggatcg ttcggtcgat atgatttctg cttcgacgac gagcttgatg
     2461 aagcgacgat ggctcatctg cacagcgaaa cggagcgcta ctatgcaaat atcgtctctt
     2521 ccttctcccc cttcgtgttg cgggggatcg aaaaactgaa gggtctgaag atagcgatgg
     2581 tcgctccgag ccacggagtc gtctggcgtt cccatccgga agcgatcatc aaccactaca
     2641 taaaactcgc ttcctacctc aacgggccgc gggaaaaaga ggtcgcgctg gtgtggtcga
     2701 gcatgtatgg gaacacccag gcgctgctgg aatcgatcat cgccggagtc gagagcgagg
     2761 ggctgccgat ccacgtcctc caggttcccc agacccatga atccttcgtg ctggagaagg
     2821 tctggcgaag cgaagggctc atcgtcggaa tgcccacgta tgaatacaag atgttcccgc
     2881 cggcgtatca tgtgctggac atccttgccc gcagccacgt caagggaaga aagacgctac
     2941 gcttcggctc ctatgggtgg tccggcgggg cccagaagca atacgacgag ttcgtcaccg
     3001 cgatgaagct tgaatgcgtc gccgaagcaa tcgagttcca aggttcgccg acgcaggagg
     3061 acaaagacaa agcgtttgcc cgggcgaaag agctggccag gatcatcaag gcgatgtaat
     3121 ccttaccgaa tcgtctccaa tagatatcgg gtaaactcgc tgattcgtcc ggtcgaggag
     3181 atcgagaccc gctccttggt ggagtggact tcctccatgt tcggtccgaa ggaaaccgaa
     3241 tccattcccg aaatccggct gttgatgatt ccgcactcca ggccggcgtg gatcgccgtc
     3301 accttggcat cctcattcgt atactccttc cacgcccggg ccaccaaggc ggcaagcgga
     3361 gagtggatgt tgggggtcca ggaggggtag gcgccgctgt gtttcgtcgt cgctccggcc
     3421 agcttgaaga tcgtggcgaa gcgacgggcg acatcatcgc gggcggagag aattgaagag
     3481 cgatgactgg cgctcacata aaaccccttt tcgtccaagg cgaggatggc gaggttcgtt
     3541 gaagtctcaa cgatgccctt aatcgacatg ctcattgcat ctacaccgtg aggagcggca
     3601 tagagggcat caaacagggt atttgcgacc acgcttcttt taggcgtctc tacctttgta
     3661 acatcaatgg cgatatgggg gtccgccact tcgaattcac ctttgacttc tttgagaacc
     3721 gtctccacta cttggtagag ccgatcttca tcctctttgg gaattgaaaa accgatcgtg
     3781 gcggtggagg ggatcacgtt gcgtttcgag ccgccggtcg ccttgaagac ccggtactca
     3841 tccagcc
//
LOCUS       DAOPUZ010000055         3809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_24316_length_3809_cov_3.566329, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000055 DAOPUZ010000000
VERSION     DAOPUZ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24316_length_3809_cov_3.566329"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PKN79_01680"
     CDS             <1..360
                     /locus_tag="PKN79_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ94504.1"
                     /translation="LAATIRGLVVNPQRESIKKAKARGILIGAGSDTLGSVLNELLIF
                     EECGFTRKECLDTATCNAAKILHLEDKLGYVREGYIADLVLVDGNPLEDLRNLSNVRK
                     VFKGGNEVTLEWLMNLQ"
     gene            377..1369
                     /locus_tag="PKN79_01685"
     CDS             377..1369
                     /locus_tag="PKN79_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HNZ94505.1"
                     /translation="MDDKLVTIIGSEPLSLSLAAQYAERGERVCYVDLAKNRLLKETK
                     TLHVQGIWNTRVELNDVLFSLEEMNPSETVVIAVPPSKFSQVFPVVLKNVREHDTVIF
                     FPASFGAISFMEQLEEKGLANDVTVAEAVSYPFVCAQKDSNTIISQSHKSELRVAVSP
                     ESRTDSFISELNEVFDIFSVARNFLETSLDNINMTIHPLPVLLNIGAIERDASFRHYI
                     DGVTPTVGKLLDKIDAERMAIGEKLQVKLTSALDQLKKYYGDSNAANLFEYVSNENGP
                     YTKVGRFGLDSRYVMEDIPNLLVPTTKIADTVGVEVPVMKLCITLAETVLGVTF"
     gene            1385..2362
                     /locus_tag="PKN79_01690"
     CDS             1385..2362
                     /locus_tag="PKN79_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNZ94506.1"
                     /translation="MLVPVVLFIVGLVLLIKGGDWFVDGAVGIAHRYHIPEILIGATV
                     VSIGTTIPEVLVSSIAAGLGQSQTAYGNAIGSVICNTALIAAITIAVKPADTVDTGTF
                     RIPVFFFFGAAALYSAVAYTTGQFTRVVGSILLSVFVVYMMINTKKAFSGELVLDVEE
                     DDEKEAKESSLGKDLLLLILGAAVIAVGADLLVKNGKIIAEGLGVPESVIALTFVALG
                     TSLPELVTAITSLAKGHGSLSLGNIVGANLFNLVLVSGLAVTIRPFGIPTEKMIAGHN
                     ASLLVDLPVMFAVMVLMTLPALLKKRLYRWQGIMLLCIYAAYVSFQFFF"
     gene            complement(2365..>3809)
                     /locus_tag="PKN79_01695"
     CDS             complement(2365..>3809)
                     /locus_tag="PKN79_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94507.1"
                     /translation="EEALALARTMQNPAMVAMMGPVYGLDAISQAIVMSQETLLWYLI
                     AISVMNIFLVTRHTRTDEELGRVELLLAQPIKPKTPTIAPILFALIVNTVIGILSIIG
                     LLIVDLEGTTFSGAFTFGVAIGNTGFFFAALTLLLAQLFATSTGVLTASLSFLGVSYI
                     IRAFGDLQESPLTLFSPFGLALKVRAFYLNDLWPIPILLLQALVLIALALLFNHVRDH
                     GAGIIPQRASRIHAPKSLLTPLGFAWRLGRSVTIAWGSALFILGLSYGSVIGELETFV
                     QSNELMRTIILQGGPDTLTDGYVALIFALMSMVAGIPVMLSALRIRKEELRGRSELIF
                     ARPIDRIGVYLPFLFIAFIEGILMTLLLALGLVLGSNNALALGEMVCHGLTYLPAIWL
                     LGGIATFLVGMLPSLTSTVWILFASFFTISYVKQITPIPFWLSALNPFELIPRVPIEP
                     FKAKPLIVLTIISAFLVAGGLYRFEKRDLS"
BASE COUNT          937 a          879 c         1115 g          878 t
ORIGIN      
        1 cttgcagcca cgataagagg gcttgtcgtg aaccctcagc gcgagagcat caagaaagca
       61 aaggcccgcg gaattctgat cggagcggga agcgatacct tgggatccgt tctcaacgag
      121 ctgctgatct ttgaggaatg cggctttact cgcaaggagt gtcttgatac ggcgacatgc
      181 aatgctgcaa agattcttca tctggaggac aagctcggat atgtaagaga aggctatatc
      241 gcggacttgg tgcttgtcga tggaaatcct ctggaggatc ttcgtaatct ttccaatgtg
      301 agaaaggttt tcaagggcgg gaatgaggtt actcttgagt ggcttatgaa tcttcagtga
      361 gacagttcat agaagaatgg atgataaact cgttaccata atcggatcgg aacctctttc
      421 tctttctttg gcagcacagt atgcggaaag aggagaaagg gtctgttatg tagatcttgc
      481 aaagaaccgg cttctgaaag aaacgaaaac ccttcatgtc cagggaatct ggaatactcg
      541 agtcgaactc aatgacgtgc ttttctcttt agaggaaatg aacccttccg agactgtcgt
      601 gattgcggtt cccccatcga agttttcgca ggttttcccg gtcgttttga aaaacgtgag
      661 ggaacatgat acggtcattt tctttcccgc aagttttggg gcgatcagtt ttatggaaca
      721 attagaggaa aaagggttgg caaatgacgt tactgtggct gaagcagtat catatccatt
      781 tgtttgtgct caaaaagatt cgaatacaat catttctcaa agccataaga gtgagttgag
      841 agttgcagta tcccctgaaa gcagaacaga ttcttttatt tctgaactta atgaagtttt
      901 tgatatcttc tcggttgcaa gaaattttct ggaaaccagt ttggataaca taaatatgac
      961 aatacatcca cttccggtgc ttcttaacat cggagcaatt gaaagagacg cttcattcag
     1021 acattacatt gatggagtga ctcctaccgt tggaaagctt cttgataaaa ttgatgcgga
     1081 aagaatggca atcggagaaa agctccaagt aaaactgact tccgcactcg atcagcttaa
     1141 gaagtattat ggtgattcga atgctgcaaa tcttttcgaa tacgtaagta atgaaaacgg
     1201 tccttacaca aaagtcggtc gctttggact tgacagcaga tatgtaatgg aggatattcc
     1261 aaacttgctg gttccgacta ccaagatcgc agatacggta ggggttgagg ttcccgtgat
     1321 gaaactctgc ataactcttg ccgaaactgt tttaggggtt acattctaag atttaggaga
     1381 tgccatgctg gttcccgtag tattgttcat cgtgggtctc gtcctcctga tcaagggggg
     1441 agactggttc gtggatgggg cggtgggaat cgcccacaga tatcatatcc ccgagatcct
     1501 gatcggggcc acggtcgtgt cgatcggaac gacgattccg gaagttctcg tctcatcaat
     1561 tgctgcaggg cttggccaga gccagacggc gtacggaaat gcgatcggat cggtgatctg
     1621 caacactgcc ctgatcgccg cgatcacgat cgcggtgaaa ccggcggata ccgtggatac
     1681 ggggaccttc aggattccgg tgttcttctt ctttggcgct gcggcactct attcggcggt
     1741 cgcctatacg accggtcagt ttacaagagt ggtcggatcg attcttcttt ccgtgttcgt
     1801 cgtgtacatg atgattaaca caaagaaagc attcagcggg gaactggtcc ttgatgtaga
     1861 ggaggatgat gaaaaggaag cgaaagagag ttctctcggc aaggatcttc tgttgctcat
     1921 cctcggcgcg gcggtgatcg cggtgggagc tgacctcctg gtcaagaacg gaaagatcat
     1981 cgccgaggga cttggagttc ctgaatcggt gatcgccctg accttcgtgg cgctgggaac
     2041 ctctttaccg gaactcgtga cggcgataac gtcgcttgcc aaaggccatg gaagcctctc
     2101 gcttggaaat atcgtcggag cgaacctctt caacctcgtg ctcgtaagcg gcctggcggt
     2161 cacaatcagg cctttcggca ttccgaccga aaagatgatc gcaggtcaca acgcatcgct
     2221 tctggtcgat cttccggtga tgtttgcggt tatggttctg atgacgcttc cggcgctgct
     2281 gaagaagcgg ctgtaccgat ggcaggggat catgctcctc tgcatctatg ccgcctacgt
     2341 ctctttccag ttcttcttct gagtctacga aagatcccgt ttctcgaacc ggtagagccc
     2401 tcctgcaacc aagaaggccg atatgatcgt gaggacgatc agcggcttgg ccttgaaggg
     2461 ctcgatgggg acgcggggga tgagctcgaa ggggttgaga gcggaaagcc agaagggaat
     2521 cggggtgatc tgcttgacgt aggagatggt gaagaaggag gcgaagagaa tccaaactgt
     2581 ggacgtcagc gaaggtagca tcccgacgag gaaggtcgcg attccaccca ggagccagat
     2641 cgccggaagg taggtcagtc catggcagac catctctcca agggcgaggg cgttgtttga
     2701 accaaggacg agaccgaggg ccaggagaag cgtcatgagg atgccctcga tgaaggcgat
     2761 gaacaggaag ggaagataga ctccgattcg atcgattggc cgggcaaaga tcagttcgct
     2821 tcggccccga agctcttcct ttcgaatccg cagggcggag agcatcaccg gaatgccggc
     2881 caccatgctc atgagtgcga agatcagggc tacatagcca tccgtcaggg tgtcgggacc
     2941 gccttggagg atgatcgtcc tcatcagctc gttggattgg acgaacgtct ccagttctcc
     3001 gatgacgctt ccgtaggaga gaccgaggat gaagagggcg cttccccaag cgatcgtgac
     3061 actccggccc aaccgccagg cgaaccccag aggggtaagg agcgatttcg gggcgtggat
     3121 tcgacttgct cgctggggaa tgatccctgc gccatggtcg cgaacgtggt tgaagagaag
     3181 ggcgagagcg atcagaacca aggcttgaag aagaaggatc ggaatcggcc aaagatcgtt
     3241 taagtagaac gcccggacct tcagcgcaag accgaagggc gagaacaggg tgagggggct
     3301 ttcctgaaga tcgccgaagg ctctgatgat ataggaaact cccagaaagg acaggcttgc
     3361 ggtgaggacg ccggtcgagg tggcaaacag ttgcgcaagc agcagcgtga gggcggcgaa
     3421 gaagaatccc gtattcccga tcgccacgcc gaaggtgaat gcgccactga acgtggtccc
     3481 ttcaagatcg acgattagca gtccgataat gctgaggatg ccgatgacgg tgttgacgat
     3541 gagggcgaag aggatcggag cgattgtcgg ggtttttggt ttgatgggtt gggccagtag
     3601 caactctacc cgtccaagct cctcgtcggt ccgggtatgg cgagtgacga ggaagatgtt
     3661 catcaccgag atggcgatca gataccacag gagggtctcc tggctcatca cgatcgcctg
     3721 gctgatcgca tcgagtccgt agaccggtcc catcatcgcc accatcgcgg gattctgcat
     3781 cgtccgggcg agggccaaag cctcttcat
//
LOCUS       DAOPUZ010000056         3779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_24578_length_3779_cov_2.416488, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000056 DAOPUZ010000000
VERSION     DAOPUZ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24578_length_3779_cov_2.416488"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2896
                     /locus_tag="PKN79_01700"
     CDS             <1..2896
                     /locus_tag="PKN79_01700"
                     /EC_number="3.1.4.4"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF023335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004630 - phospholipase D activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ94508.1"
                     /translation="DRRRSSSEAMRPKLVLNNRLTQNRVADVLNTHLHTCSSFSFSVA
                     FLSKGGLATLLQALLECKRRGGTGRMLTSDYLNFTDPRAVGFIFRELGPEIEVRVYTA
                     EAFHTKGYLFEHEERPTTLIIGSSNITQDALLRNHEWNLQVAGTNQGQLIQDTKEEFE
                     RLWTNATTVSEAWLEHYTKVWDTFNTARVFVPRQVDSRSIQETSVAKRGFELNSMQKD
                     ALARLKEIRSEGMSKALLVSATGTGKTILAASDVNQVKPERFLFIIHREQIAHEAMRS
                     FMQHLDEPVECALLTGSDRNLNAPYLFATIQTLTKDEVLYSFDPDWFDYIVVDEAHHA
                     PAASYRRVLEYFRPAFLLGMTATPVRPDGRDVHGIFGNTVAAEITLSQALEEDLLAPF
                     HYFGITGLSIDGTEGQKVEEFSNLEREEQIKHLSVILRRYSLGLSRRRGLIFTSTNDD
                     AAFVARELRGLGVRALQLSGASSQKEREEAFDRLEAEEEDPHHLEYLVSVDIFNEGVD
                     IPALNQIIMLRPTQSVIVFTQQLGRGLRKYPGKEFLTVVDFVGNHQNNYMIPIALFGE
                     NSYDKDTLRRVVFGGAAIPGVSTVTFDRIAKERILDSINRASFSNRRMLTEAYQSVKN
                     RLGNRIPSLVDFIRFDGINPELFLRYRRTWAQCKAVIDPAFTLAMDDIHLKSLRFFSL
                     YLPRGIRILEAGILSALLEGPKTEEALFARFKTNYGFVPTVASFASALRILNNEFVQE
                     KYKESYGNISYVEEADGRYRLSREFSELLRNDQYRSELNELIQLGTLNHQLRFAGQCD
                     DFGLVRGRKYTRSEVCRVLNWESDEGNTIYGYKVHGETNTCPIFVTYHKHEGIDASID
                     YDDLFIDHQHFAWQSRTRVRLGSTEMQGIRDPEMKKLLFVKKRDDEGAGHYYLGELGF
                     LSNEQSSRLNNEGVELDVVNVRFRLFHPVEEKLYDYITDREQEEMVALDA"
     gene            complement(2893..>3779)
                     /locus_tag="PKN79_01705"
     CDS             complement(2893..>3779)
                     /locus_tag="PKN79_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94509.1"
                     /translation="LVEIRRMIEKQSVLQDRYEKQADQVRRYASTNHIRQTDLPAQAT
                     SFTTSALANYLGVIIAAHLGEQSTLDYSFNQIHHAFETQTKLYPFPIPESVEPLKSEG
                     RDSVHLIAFTGRFPVKEERMDFISISPTNVTRIAYPVLRPVPSAITSIRVRVDGKVIT
                     TLEKIESFSLVAEDAFQSKSGLLYAKATMRAISKELGIGIWDAISKDKDGKRSAFAEV
                     LGFAFRVGKELSESADLRSTHYLPAEAWVGTLDLEPGSYTIEVEYLNRIGQVLHTERF
                     METIDPTTINLLESYSPL"
BASE COUNT          884 a          983 c         1071 g          841 t
ORIGIN      
        1 cgatcgccga agatccagtt cggaagcgat gcgccccaag cttgtactca ataaccgact
       61 gactcaaaac cgagttgccg atgtgctgaa cacccatttg catacgtgtt cctccttttc
      121 cttcagcgtc gcatttttaa gcaaaggagg gctcgcaact ctcctgcagg cgcttctcga
      181 atgcaaacgg cgggggggga cgggacggat gctcaccagc gactatctga acttcaccga
      241 tccccgagcc gtcggcttca tttttcgcga gctcggaccg gaaatcgaag tacgggtgta
      301 taccgccgaa gcgttccaca ccaaagggta cctctttgaa catgaagaga ggccgacgac
      361 cctgatcatc ggatcatcca atatcactca agatgccctc ctcagaaacc atgagtggaa
      421 tcttcaggta gccggaacca atcaaggcca attgattcag gatacgaagg aggagtttga
      481 gcgtctttgg accaatgcaa ccactgtctc ggaggcgtgg ctcgagcact acaccaaggt
      541 gtgggatacg ttcaataccg ctcgagtatt tgtacctcgg caggttgaca gccgttccat
      601 tcaggagact tccgtcgcca aacgcggttt cgagctcaat tcgatgcaga aagatgctct
      661 tgcgcgtctt aaagaaattc ggtcggaagg aatgagcaaa gccctccttg tttcggcaac
      721 gggcacggga aaaacaatcc tggcggcaag tgatgtgaat caggtaaaac ctgagcgatt
      781 tctgttcatc atccaccgcg aacagattgc ccatgaagcg atgcggagtt tcatgcagca
      841 tctcgacgag ccggtggaat gtgccctcct gaccggctcg gatcggaacc tgaacgcccc
      901 ctatctcttt gcaacgatcc aaacgctcac aaaggatgag gtgctgtact cgttcgaccc
      961 cgactggttc gactacatcg tcgtcgatga ggcccatcac gctccggcgg caagctacag
     1021 gagagtcctt gagtattttc gcccagcatt tctcctgggg atgaccgcca cacccgtccg
     1081 accggacggg agggatgtcc atggaatctt tggcaatacg gttgcggcgg aaatcaccct
     1141 ttctcaggcg cttgaagaag accttctggc tcccttccac tacttcggca tcaccggtct
     1201 cagtatcgat ggcactgaag ggcaaaaagt cgaagagttc agcaatcttg aaagggaaga
     1261 gcagataaaa catctttccg tcatcttgag gcgatactcc ttgggcttga gtcgaagaag
     1321 aggactcatc ttcacctcga cgaatgacga tgccgcgttt gtcgcccggg aattgcgggg
     1381 ccttggcgtt cgggcgcttc aactcagtgg agccagcagc cagaaagagc gggaagaagc
     1441 gtttgatcgt cttgaagcgg aagaggaaga tccgcaccac cttgaatatc tggtgagcgt
     1501 cgatatcttc aacgaggggg tggatattcc cgcgctcaac cagatcatca tgctccggcc
     1561 gacgcaatcg gtcatcgtgt ttactcagca actcggaagg ggactcagaa aataccccgg
     1621 aaaagaattc cttaccgtcg tcgatttcgt gggaaaccac cagaacaact acatgattcc
     1681 catcgccctc ttcggggaga acagttatga taaggatacg ctgaggcggg ttgtcttcgg
     1741 aggagcggcg atccccggcg tttcgacggt cacgttcgac cggatcgcga aggaacggat
     1801 cctcgattcg atcaacaggg cgtcgttctc caacaggagg atgctgaccg aagcgtatca
     1861 aagcgtaaaa aaccgcttgg ggaatcggat accgagcctc gtggatttca tccgcttcga
     1921 tggcatcaac ccggagctct tcctccgcta ccggagaaca tgggctcagt gcaaggctgt
     1981 gatcgatccc gctttcaccc ttgccatgga tgatatccat ctcaagagcc tgaggttttt
     2041 cagcctttat ctcccacgcg gtatcagaat ccttgaagcg ggtatcttgt cagcacttct
     2101 ggaaggtccg aaaacggaag aagccctgtt tgcccggttc aaaacgaact atgggtttgt
     2161 accaaccgtt gcttcgtttg catcagctct tcggatcctg aacaatgaat tcgtccagga
     2221 gaagtacaag gaatcctatg gaaacatcag ctacgtcgag gaggcggatg ggcgctatcg
     2281 cctttcaagg gagttctcgg agcttttgcg gaacgaccag tatcgaagcg aactgaatga
     2341 acttatccag ctgggcacgc tgaaccatca gttgcggttt gccggacagt gcgatgattt
     2401 cggtcttgtg cgtggacgga agtatacccg cagcgaggtg tgccgagtgc ttaattggga
     2461 aagtgatgaa ggcaacacga tctatgggta caaagttcat ggggaaacga atacgtgtcc
     2521 gatcttcgtg acgtaccaca aacatgaagg tatcgatgct tcgatcgatt atgacgatct
     2581 cttcatcgac catcaacact ttgcatggca gagtcggacc cgggttcggc tcggatcgac
     2641 agagatgcag gggatccgag atccggagat gaaaaaactc ctgttcgtca aaaagcgtga
     2701 cgatgagggg gcgggacact actacctcgg cgagctcggc tttctttcga atgagcagag
     2761 ttctcgccta aataatgagg gtgtcgagct ggatgtggtg aatgtccggt ttcgcctttt
     2821 tcacccggtg gaggagaagc tctacgacta tatcaccgac cgggaacagg aagagatggt
     2881 cgccctcgat gcctagaggg gactgtacga ttcaagcagg ttgatcgtcg tgggatcgat
     2941 cgtctccatg aagcgctcgg tgtgaagcac ctgtccgatt cgattgaggt attccacctc
     3001 gatcgtgtag gaacccggtt cgagatcgag cgttcccacc caggcttctg ccggaaggta
     3061 gtgcgtcgaa cgaagatccg ccgattcgct cagctctttg cccactcgga atgcaaagcc
     3121 gaggacctcg gcaaaggcgc tccgcttgcc gtccttgtcc ttcgagatgg cgtcccagat
     3181 gccgatgccc agctctttgg agatcgcccg catggtcgct ttggcgtaga gcagaccgct
     3241 ttttgattga aacgcatcct cggcgacgag ggagaatgat tctatttttt ccaaggtggt
     3301 aatgaccttt ccgtcaaccc ggacccggat tgaagtgatc gccgaaggaa ccggccggag
     3361 aaccgggtag gcgattcggg tgacgttggt gggggagata ctgataaaat ccatccgctc
     3421 ctccttgacg ggaaagcgtc cggtgaaggc gatcaggtgg acggaatccc gaccttcgct
     3481 cttgagcggc tcgacacttt cgggaatggg gaaggggtag agcttcgttt gggtttcgaa
     3541 cgcatggtga atctgattga aggagtaatc gagggtggac tgttcaccga gatgggcggc
     3601 gatgatcacc ccgaggtagt tggcgagcgc actggtggtg aacgaggtgg cctgagcggg
     3661 cagatcggtt tggcggatat ggttggttga cgcataacgg cgcacctgat ccgcctgctt
     3721 ttcgtagcga tcctgcagca cgctctgctt ttcgatcatc cgccgaatct cgaccaaac
//
LOCUS       DAOPUZ010000057         3752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_24828_length_3752_cov_2.961861, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000057 DAOPUZ010000000
VERSION     DAOPUZ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24828_length_3752_cov_2.961861"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PKN79_01710"
     CDS             <1..705
                     /locus_tag="PKN79_01710"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNZ94510.1"
                     /translation="KAIALGRALRAAHTLKIRQPLSKLFLVDRNEHERKVLASMQESI
                     AEELNVKSVSIEADETALVTYSAKANFKLLGGKLGKDMKEVAALIQELDSKTIARILD
                     GGVYPLHYSNGTIDLGVEEIIVQRSEIEGVKVINDGTLTVGFDTTMTEELLDEGLMRD
                     LIRAIQNLRKESGFDVTDRIMIAYEGDDKIHDVFTKFESIISDETLADEITSKPLDGE
                     ALQLQEYQVRLELKQV"
     gene            705..1568
                     /locus_tag="PKN79_01715"
     CDS             705..1568
                     /locus_tag="PKN79_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94511.1"
                     /translation="MKKAKIVLLALFAVMLLGSCTTTTVRKRPAFTIEELPMHRTILV
                     TTNDPGIIQTVLPDVADRSERISVVIEPEDDAYPLNLEASRIYGVMETTYSRVAMNTA
                     LMWSSKTKKDLHEDPSYFQMKESDLELYSPKNGKLLFTLDDYPAVYEHLSKEDGKRLS
                     AEIAERMKESAIALYAEYPRTFFDLNLGVSEEVVRQSESILLLFDRSEEGGYVLNAFI
                     TMGDEKKAKTLSQMVRSGYIANLKKANQKYVIAELMKMFLLDGQLVTIKGIEITEDEL
                     FKILAGLDGLI"
     gene            1581..1751
                     /locus_tag="PKN79_01720"
     CDS             1581..1751
                     /locus_tag="PKN79_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ94512.1"
                     /translation="MPSYEYRCRLCKATIELVQSVGKHQVPEVCPSCNMKHTMVRVNI
                     PVSTKEKEGEKR"
     gene            1748..2737
                     /gene="trpS"
                     /locus_tag="PKN79_01725"
     CDS             1748..2737
                     /gene="trpS"
                     /locus_tag="PKN79_01725"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNZ94513.1"
                     /translation="MRSRRRILTGDRTTGKLHLGHYVGSLKSRVELQDEYETFILLAD
                     IQALTTHFNEPELINSSILDVTMDNLSVGLDPEKVTFVQQSQITSIAELTVFYSMIVS
                     VNQLRHNPTIKTEAANYGYEDLSYGFLGYPVSQTADISFINADLVPVGEDQVPHIELS
                     RKIIRKFNKLYGTNITVPRAKVSEVGRLSGLDGNAKMGKSMGNAIYLSDSPEVVWQHV
                     RKAVTDTSRVHAHMEGHPEVCNVYKYHQVFNPEEADEICKGCTSAALSCFACKQRLNE
                     VLNNLLEPMRERRAYYENNIDIVKELIHEGSKKANSIGNENLERIKEKMHILI"
     gene            2739..3608
                     /locus_tag="PKN79_01730"
     CDS             2739..3608
                     /locus_tag="PKN79_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ94514.1"
                     /translation="MKKDSQYLKGILITFVGVLVLSSDALLIRMSSVPGFRAAFWRGL
                     FTFLSVSIIFLITNKKEAATIVKTQYKRMLFPALLWGSSGVAFAIGVHNSGASIALVM
                     ISLGPFFASFQSFLFYREKPHPLTLLAALGAVGGIVYMFGSQLGTLEAKDFFYNIWSP
                     LLYGTNLAYLRNNPTLNRQGISILGGFTAMILALFISRFHIVIPFKNLLPLIVLGAIV
                     IPFAQLSIGHGTRFIPAAESALISSLETIIGIIYVWIFLNEAPSLHTILGGAIVFSCI
                     VFNTVVQAYRVKR"
BASE COUNT          937 a          959 c          981 g          875 t
ORIGIN      
        1 aaggcgatcg ccctcggccg tgccctgagg gccgctcaca ccctgaagat ccggcagccg
       61 ctctccaagc tcttcctcgt cgatcgcaat gaacatgagc gcaaagtgct cgcttcaatg
      121 caggagagca tcgccgaaga gctgaacgtg aagagcgttt caatcgaagc ggatgaaact
      181 gcgcttgtca cctacagcgc gaaggccaac ttcaagctgc ttggcggaaa gctcggtaaa
      241 gacatgaagg aagtggcggc tttgatccag gaactggatt caaagacgat tgcccgcatt
      301 cttgacggag gggtctaccc gcttcattac tccaatggta cgattgatct gggtgtcgag
      361 gagatcatcg tccagcgaag cgagatcgaa ggagtgaagg tgatcaacga cggaacgctc
      421 accgtcggct tcgatactac gatgaccgaa gagctgctgg atgaaggatt gatgcgcgat
      481 cttattcgag cgattcaaaa tcttcgaaaa gagagcgggt ttgacgtcac cgaccggatc
      541 atgatcgctt atgagggtga tgataagatc cacgacgttt ttacgaagtt tgaatcgatc
      601 atcagcgatg aaacgcttgc ggatgaaatt acctcaaagc cgcttgacgg ggaagccctt
      661 caactgcagg aatatcaagt tcgtttggag ctcaagcagg tatgatgaag aaagcaaaga
      721 ttgtactgtt ggctctgttc gcggtgatgt tgcttggcag ctgcaccacg acaacggtgc
      781 gcaagcggcc ggcattcacg attgaagagc tgccgatgca tcggacgatt ctggttacga
      841 ccaacgatcc cggaatcatt caaacggttc ttcccgatgt tgcggatcga agcgaacgga
      901 tcagtgtcgt catcgagccc gaggatgatg cgtatccgtt gaacctcgag gcaagccgaa
      961 tctatggggt gatggagacg acgtactcaa gagttgccat gaacaccgca ctcatgtggt
     1021 cttcaaaaac gaaaaaagat ctccatgaag acccttccta cttccaaatg aaggaaagcg
     1081 atcttgagct gtattcgcca aaaaacggca agctcctgtt caccctcgat gactacccgg
     1141 ctgtgtatga gcacctttcg aaagaagacg ggaagcggct ttccgccgag atcgccgaaa
     1201 ggatgaaaga aagcgccata gccctctacg ccgagtaccc aaggacattc ttcgatctca
     1261 atctgggagt cagtgaagag gtggttcgcc aaagcgagtc gattcttctc ctctttgatc
     1321 gaagtgaaga aggcgggtat gtcctgaatg cgttcatcac gatgggcgat gagaagaagg
     1381 cgaagactct ttcgcagatg gttcgcagcg gctacatcgc gaacttgaaa aaagcgaatc
     1441 agaaatacgt gattgctgaa ttaatgaaga tgttcctcct tgacggtcag cttgtgacaa
     1501 tcaaaggaat agagataacg gaagatgagc tgttcaagat ccttgcgggc ttggatggct
     1561 tgatatagag gagtccgctc atgccgtcat atgagtacag atgccgctta tgcaaggcaa
     1621 caattgagtt ggtgcaatca gtcggtaagc accaggtacc cgaggtgtgc ccttcctgca
     1681 atatgaagca cacgatggtc cgggtgaata tccctgtctc aaccaaggag aaagaggggg
     1741 agaagagatg agaagccgaa gacgaattct caccggggat cgaaccacgg gaaagctgca
     1801 cctcggtcac tatgtcggtt ctttgaagag ccgggtggaa ctgcaggatg agtacgagac
     1861 gttcatcctc cttgccgata ttcaggcgct caccacccac tttaacgaac ctgaattgat
     1921 taattcatca attcttgatg tcacgatgga caacctctca gtagggctcg atccggaaaa
     1981 ggtaacattt gttcaacagt cccaaatcac ctcgatcgcc gaactgaccg tcttctactc
     2041 gatgatcgtc tccgtcaatc agctgcggca caatcctacg atcaagaccg aagcggccaa
     2101 ctacggctac gaggatctct cctatgggtt cttgggatat ccggtcagcc agaccgccga
     2161 tatctccttc atcaacgcgg atctcgtgcc cgtcggtgag gaccaagtcc cccatatcga
     2221 gctttcccga aagatcatcc ggaagttcaa caagctctac ggtacgaata tcaccgttcc
     2281 ccgggcgaag gtcagtgaag tcgggcggct ttcgggtctt gacggcaatg cgaagatggg
     2341 caagtcgatg ggcaatgcga tctacctctc cgattccccc gaggtcgtct ggcagcacgt
     2401 ccgcaaggcg gtaaccgaca cctcccgggt tcatgcccat atggaagggc atcccgaggt
     2461 gtgcaacgtc tacaagtacc atcaggtctt caaccccgag gaggcggatg agatctgcaa
     2521 agggtgcacc agcgcagcgc tttcctgctt tgcctgcaag cagcggctca acgaggtgtt
     2581 gaacaatctg cttgagccga tgcgcgaacg ccgtgcctac tatgagaaca acatcgatat
     2641 cgtcaaagaa cttatccatg aaggatcaaa gaaggccaat tcgatcggca atgagaacct
     2701 tgagcggatc aaggaaaaga tgcacattct gatctgacat gaagaaggat tctcagtacc
     2761 tcaaaggaat cctcatcacg tttgtggggg ttcttgtttt aagcagtgat gccttgctta
     2821 ttcgaatgag cagtgtgccc gggtttcgcg ccgctttttg gaggggactg tttaccttct
     2881 tgtccgtgtc aatcatcttt ctcatcacca acaagaaaga ggccgcaact attgtaaaaa
     2941 ctcagtacaa gcgaatgctc tttcccgcct tgctgtgggg ctccagcggt gttgcatttg
     3001 cgatcggagt acacaacagc ggtgcttcta tcgctttggt gatgatcagc ctcggtccgt
     3061 tcttcgcttc gtttcagtcc tttctgttct accgggaaaa accccacccg ttgactctgc
     3121 ttgccgccct cggagcggta gggggaatcg tctatatgtt cggctcgcag ctcggcaccc
     3181 ttgaagcgaa agatttcttc tacaacatct ggtcaccgct cctgtatgga accaatcttg
     3241 cctatctacg caataatccg acgctcaacc gccaggggat cagtattctg gggggattca
     3301 ccgcgatgat attggcactc ttcatctccc gattccacat cgtcatcccc ttcaagaatc
     3361 tcctcccgct catcgttctc ggggcgatcg tgattccgtt cgcccagctc tcgatcggac
     3421 acggaacccg gttcatcccg gctgcggaga gtgccctgat cagcagtctg gaaacgatca
     3481 tcgggattat ctatgtctgg atcttcctaa acgaagcccc gtcccttcac acgatcttag
     3541 ggggagcgat cgtcttctcc tgcatcgtct tcaatacggt cgtccaagcg tatcgggtaa
     3601 agcgttaaaa agaaagatga acctttggaa catatttgtt catctcttat tgcggcaaac
     3661 atgcatctat gggataatgc ggtttatacg tttttgctca agcggtgtgg attttgataa
     3721 ttgccgaaca tgttgatctc gtaacgtctt ct
//
LOCUS       DAOPUZ010000058         3748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_24861_length_3748_cov_3.158137, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000058 DAOPUZ010000000
VERSION     DAOPUZ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24861_length_3748_cov_3.158137"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..867
                     /locus_tag="PKN79_01735"
     CDS             91..867
                     /locus_tag="PKN79_01735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ94515.1"
                     /translation="MTTYRTAAWEEFYELKDLWNVVFNEEPAFLEKFFSTRFDPNHVF
                     VAEDEGRIVSALHSLPSSYTKEGITNPCSFIVGAATLQSHRNRGIMSRLLERVQASYP
                     HPITLFPALRPYYEKHGYFTTSTARAYAIPPTLALNERQKRFATPDDLEPIYRSATYR
                     CGSLDRDELAWGFLIDGYETLYVEDGYAFILEDKAVEAFALNREAARLLLGILARRGI
                     STIHALSASPFESFLENGYPVPMGMATDKALEGVYIAEQY"
     gene            complement(904..1578)
                     /locus_tag="PKN79_01740"
     CDS             complement(904..1578)
                     /locus_tag="PKN79_01740"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNZ94516.1"
                     /translation="MEEIVKTTGLPLIGDPAPAFRAVTTQGEINFPEDYKGKWVILFS
                     HPADFTPVCTTEFMTFASMHDEFKAMNTELVGLSIDSLYAHIAWLRKIQELEWKGMKN
                     IEVKFPVIEDIKMDVSRKYGMVQSQSSTQAVRAVFIIDPKGIVRTILFYPAAIGRNFD
                     EIKRAVQALQKADKDGVATPADWRPGQDVIIPTPGSCGTAKERMESQDEKMYCLDWFL
                     CFRKES"
     gene            1735..>3748
                     /locus_tag="PKN79_01745"
     CDS             1735..>3748
                     /locus_tag="PKN79_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNZ94517.1"
                     /translation="MRKGGIIIKKQPRFVQLFVIGIVIVLLALAVMMHINFMVNHRVS
                     DLNKRIMDANHLITELYTLEHIFQSIRLAQRGHLLAGTDDYLEAFETQNQAYVSTLSK
                     IECVVKNDPEQHRLLQEIRNTYNATLIYHITPLFERRSVFEDDPSLIFKDDIIQDLLA
                     HSSNASFTLQTLIGTLQEHERMSLEKAYDDFSFWQYVNWVSIFLIPSVGIPLLFVGSF
                     LFLRELHRYRTGQEKHLESLRLERDRYEEAIRCSNLFSYSWDLENDVITPEDRFIRLL
                     GYDPERIQGMTAIQYSQFFHPDDIGKLAIDYQTHLMGKSEHLTTMVRMIHKDGHPITF
                     MLRGQISRRSSDGEPLEIVGTYNDITEQVTLALAPKQDMKDLKAMFDTMDQGLAYMRL
                     IHNEAGEVVDFEIVRANRAHSEFIGVPNEEILHRPASTLSPPLSKELVELNLNVGITG
                     KSVIFETKDLLFGRYFRISSYQPEPGFVAMLMDDITNERDMEHKVLYERMLFETTLLS
                     VAEGVISTDEVGIVQFINEAAEHLIGWEAEEAIGRPLSEIFHLVPTDRRKRTPDLAQL
                     VLSKRESLSLGDQATLVARDGVERYISDHASPIIGKDGELYGMVIVFRDATEDRTKAQ
                     EMRTLSISDPLTSLYNRRHFDRIKEEINNEPYQPLTLVLADVNGLKL"
BASE COUNT          855 a         1057 c          964 g          872 t
ORIGIN      
        1 ccaggaggat gccggcatcg atggtctcag gaaagcgaag ctctcctacc agccggacct
       61 gatggtcagg aaatatcgga taacgaagcc atgaccacct atcgaacagc ggcttgggag
      121 gagttttatg agctcaaaga tctgtggaac gtcgtcttca acgaggagcc ggcttttctt
      181 gagaagttct tttcgacccg cttcgatccg aaccatgtct tcgtagcgga agatgaggga
      241 aggatcgttt ccgccctcca ctcccttcca agctcctata cgaaagaggg aattacgaac
      301 ccctgctcct tcatcgtcgg agcggcgacg cttcaatccc atcggaaccg gggaatcatg
      361 agcaggctgc tggagcgtgt tcaagcttcc tatccccatc cgatcaccct ctttcccgcc
      421 ctccggccct actatgagaa gcatgggtat ttcactacgt cgaccgcccg ggcgtatgcg
      481 atcccgccca ccttggcgtt gaacgagcgc caaaaaaggt tcgccacccc ggatgacttg
      541 gaaccaatct atagaagcgc gacgtaccgc tgcggctcgc tcgaccggga tgagctggcg
      601 tgggggtttc tcatcgacgg gtatgagacg ctctatgttg aagacggcta tgcgttcatc
      661 ctcgaggaca aggcggtcga agcattcgcg ttgaacaggg aagcggcccg cctgctctta
      721 ggcatccttg cccgacgggg aatctcaacg attcatgccc tgtccgcttc gccgttcgaa
      781 tcatttctgg agaacggcta cccggttccg atgggcatgg ccaccgataa agcccttgag
      841 ggcgtctaca tcgctgagca atattagagg gagccggtga aagctccccc ctttccgtga
      901 tccttacgat tccttcctga agcagaggaa ccagtccaaa cagtacatct tctcgtcctg
      961 actttccatc cgctccttcg ccgttccgca ggagccggga gtcggaatga tgacatcctg
     1021 accgggtctc cagtccgccg gggttgctac gccatccttg tccgccttct ggagcgcctg
     1081 aaccgcccgc ttgatctcgt cgaagttccg gccgatcgcg gcgggataga agaggatcgt
     1141 ccggacgatg cccttcgggt cgatgatgaa gaccgctcga accgcctgag tgctgctctg
     1201 gctctgaacc atcccgtact tgcgggagac atccatcttg atgtcctcga tcaccgggaa
     1261 cttcacttcg atgttcttca ttcctttcca ttcgagctct tggatctttc gcagccacgc
     1321 gatgtgagcg tacagcgaat cgatcgaaag cccgacaagc tcggtgttca tcgccttgaa
     1381 ctcatcgtgc atcgaggcga aggtcatgaa ctcggtggtg cacacgggag tgaagtccgc
     1441 ggggtgactg aacaggatga cccactttcc cttgtaatcc tccggaaagt tgatctctcc
     1501 ctgggtggta accgcccgga acgccggagc ggggtcgccg atcaagggca aaccggttgt
     1561 tttaacaatt tcttccatgg ttttactcct catagtaata ctgatacctt aatgatacta
     1621 attatcatta ataaaggcaa acactttctc tctccagatt gatactcctt ttgattttcg
     1681 gtatcgcatg ggggtatata tttttttctt gttttagcga cgtctcttgc tataatgagg
     1741 aaagggggaa tcatcattaa aaagcaaccg cgctttgttc agctttttgt cattggcatc
     1801 gttatagtcc ttcttgcact ggcggtcatg atgcacatca acttcatggt gaatcatcga
     1861 gtcagcgacc tcaacaagcg gattatggat gccaatcatc tgatcacgga gctgtataca
     1921 ttggaacata tctttcaatc gatccggctt gcccagcgcg gacaccttct tgcgggaacg
     1981 gatgattacc tcgaagcgtt tgaaacccag aatcaggcat acgtctccac actttcgaaa
     2041 attgaatgtg tggtcaagaa tgatccggag caacaccgcc tgcttcagga aatccgaaac
     2101 acctacaatg cgacgcttat ttaccatatc actccgctgt tcgagcgccg gtcggtcttt
     2161 gaagacgacc cttcgcttat cttcaaagat gacattattc aagatttgct tgctcattca
     2221 agcaacgctt catttacgct gcaaactctt atcgggacgc ttcaggaaca cgagcggatg
     2281 agccttgaaa aggcatatga cgatttttca ttttggcagt atgtaaactg ggtaagcatc
     2341 ttccttattc cctccgtagg catcccgctg ctgtttgtcg gaagttttct tttcctcagg
     2401 gaactgcacc gctatcgtac cggtcaggaa aaacatctgg agtcactccg ccttgagcgc
     2461 gaccgctatg aagaagcgat tcgatgctcc aacctctttt cctacagttg ggatcttgaa
     2521 aacgacgtca tcacaccgga agatcgattc atcaggctgc tcggctatga ccccgagcgg
     2581 attcagggca tgacggcaat acagtattcc cagttcttcc accccgatga catcggaaag
     2641 ttggctatcg attatcagac ccacctcatg ggcaagagcg agcacctgac caccatggtg
     2701 cgaatgatcc acaaggacgg ccatccgatc acgttcatgc tccggggaca gatctcgcgc
     2761 cgctcaagcg atggcgagcc ccttgagatc gtcggtacct acaacgacat caccgaacag
     2821 gtcaccctcg ctctggctcc caaacaggat atgaaagacc tcaaggccat gttcgacacg
     2881 atggatcagg ggttggccta catgcggctg atccataacg aggcgggaga ggtggtcgat
     2941 tttgagatcg ttcgggcgaa cagggcgcac tcggagttca tcggtgtgcc gaacgaggag
     3001 attctgcatc gaccggcgtc gacgttgtca ccgccgcttt ccaaggaact ggtcgagctc
     3061 aacctcaatg tcgggattac cggcaagagt gtcatcttcg agacgaagga ccttttgttc
     3121 ggccgatact tcagaatctc ttcctatcag cccgaaccgg ggttcgtggc gatgctgatg
     3181 gatgacatca ccaacgaacg cgacatggag cacaaggtgc tgtatgaacg gatgctcttt
     3241 gaaacgaccc tcttaagcgt cgccgaggga gtgatctcga ccgatgaggt gggaattgtc
     3301 caattcatca atgaggcggc ggaacacctg atcggctggg aagcggaaga ggcgatcggg
     3361 cgtccccttt cggaaatctt ccatctggtt ccgaccgatc ggagaaaacg caccccggat
     3421 cttgcccagc tcgtcctctc caaacgggag tccctgagcc ttggtgatca ggcgaccctt
     3481 gtggcgcgag acggagtgga gcgctacatt tccgaccacg cctcaccgat catcggcaag
     3541 gacggcgagc tgtatggaat ggtgatcgtc ttccgcgatg ccaccgaaga tcgaaccaaa
     3601 gcgcaggaga tgaggactct ctcgatctcc gaccccctta ccagcctcta caaccgccgc
     3661 cacttcgacc ggatcaaaga agagatcaac aacgaaccat accaacccct taccttggtt
     3721 ctcgccgatg tgaacggtct gaagctca
//
LOCUS       DAOPUZ010000059         3746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_24879_length_3746_cov_4.145218, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000059 DAOPUZ010000000
VERSION     DAOPUZ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24879_length_3746_cov_4.145218"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PKN79_01750"
     CDS             <1..817
                     /locus_tag="PKN79_01750"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNZ94518.1"
                     /translation="RRFTTPAPLRYTEATLLSAMENAGRLVDDPLLKKQMGGGLGTPA
                     TRADIIEKLLQNAYIERDGKELVPTPKGRELIRLVPPSLKSAALTGEWELRLSNIAEG
                     IEEVGPFLSDIKESARLLVEEVKQSGEVFAPYFSESKTCPHCSSVMMKVTDPIGQAHF
                     ICQRLSCSYEEKEIKKRVFIEPEKKDPPAPRKVVVVKAAANPEAVKKRVVVKKKAPEE
                     LPYRWETTIEVVRPSKLHRRAVPQQHVFKERESSGSSFADLIRASEERKKRRR"
     gene            902..1651
                     /gene="dapB"
                     /locus_tag="PKN79_01755"
     CDS             902..1651
                     /gene="dapB"
                     /locus_tag="PKN79_01755"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNZ94519.1"
                     /translation="MKLALVGYGKMGKIIKTHALAQNHEIASIIDPFEEGAKKSLTVE
                     NLQGCDVAIDFTHPDVALEHIRFYIENGVPAVIGTTGWYEHLGEVASLVREGNGTILY
                     SGNFSLGVALFLRSVRYAAALYGKTGRYDPAIIEVHHNKKADSPSGTAGMLASRVLEG
                     FPEKTRIEYETQHQRRGDEVLHVSSLRVGSVPGTHTILFDSPEDTIELIHTARTRDGF
                     AFGAVAAAAWLNERESGLYTLEEMIDAVFLR"
     gene            1662..2573
                     /gene="dapA"
                     /locus_tag="PKN79_01760"
     CDS             1662..2573
                     /gene="dapA"
                     /locus_tag="PKN79_01760"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNZ94520.1"
                     /translation="MKTIDFRGVHTALVTPFTAKDNLDEDALQAIIEHQIESGISGLV
                     PCGTTGESPTLSHDEHDRVIALTVKFANGRVPVIAGTGSNATDEAVRLSRHAQQVGSD
                     AVLLVSPYYNKPTQKGLFEHFKAIADSVDIPCILYNIQGRTAVNIETATVVALQEACP
                     NIVGVKEASGKLQQMQEVIDSSRPEFFVLSGDDNMCLPLIEIGGHGVISVASNLLPGY
                     VSTMVEKALNGDMAEAKKMEAVISNFFRTCFLETNPIPIKTAMARYGWCNESFRLPLC
                     TFESEAKRAELYRELNHLESLKIIKRR"
     gene            2576..>3746
                     /locus_tag="PKN79_01765"
     CDS             2576..>3746
                     /locus_tag="PKN79_01765"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNZ94521.1"
                     /translation="MATINSAYRNLASGYLFPEINRRVRAWQERNPDKQVLRLGIGNT
                     TEALPPAICKAMIEKVEELGTRETYTGYGDEQGDGYLRKALVEYYQQGGVSLEMDEVF
                     VSDGAKSDAANIQTLFSIDSVVAVADPAYPVYVDSNVASGRSAGWDESRGMYRNLVYL
                     SCTEENGFIPPLPSEHVDLIYLCNPNNPTGAVMNHEQVKRFVDYARKHKSVIIYDSAY
                     SEYITEEGYPRSIYEIKGAKECAIEISSFSKFSGFTGVRLGWTIVPKNLVCEDSKPLE
                     LNRMWNRRQTTFFNGASNIAQRGGHAALSGEGYEQSRALVKYYLENARLIREGLKRVG
                     LTVYGGINSPYIWAKTPDGIDSWAFFDQLLDQCHVVVTPGGGFGPAGNRFVRVSAY"
BASE COUNT          875 a         1052 c         1128 g          691 t
ORIGIN      
        1 caggcggttc accacgcctg cgcccctccg ctacacggaa gccaccctgc tttcggcgat
       61 ggagaacgcc ggacgcctcg tggatgaccc gctgctgaag aaacagatgg gcggagggct
      121 cggcaccccc gcaacacggg cggacatcat tgaaaagctc cttcagaacg cctacattga
      181 acgggacggc aaggagctgg ttcccacccc gaaagggcgg gagcttatcc ggctggttcc
      241 ccccagcctg aagagcgccg ccctcaccgg cgaatgggaa ttacgcctct caaatatcgc
      301 cgaagggatc gaagaggtcg gaccgtttct ctccgacatc aaggagagcg cccgcttgct
      361 tgtcgaagaa gtgaagcaaa gcggggaggt gttcgctccc tatttttccg aatcgaagac
      421 ctgcccccat tgctcgagcg tgatgatgaa ggtcaccgac ccgatcggcc aggcgcactt
      481 catctgccag cggctctcct gttcctatga ggagaaagag atcaaaaagc gggtgttcat
      541 cgagccggag aagaaggatc ctcccgcccc caggaaggta gtggtcgtca aagcggccgc
      601 caatcccgaa gcggtgaaga agcgggtggt ggtgaagaag aaggcaccgg aagagcttcc
      661 ctaccgatgg gagacgacga tcgaggtcgt ccgcccctcc aaactgcacc gccgagcggt
      721 accccaacag catgtattca aggagcggga atcctccgga tcctcctttg ccgacctgat
      781 cagggcaagc gaggagcgga agaagcgaag acggtaatac tgttattata aaaacattct
      841 gttacgctgt agacactctt gtagctttct gctaggctac tctccatgcg ggaggaagcc
      901 tatgaagctt gcgcttgtcg ggtacggaaa gatgggaaag atcatcaaaa cccacgccct
      961 tgctcaaaac catgagatcg cttcgatcat cgaccccttt gaagaggggg cgaagaaatc
     1021 attgaccgtc gaaaacctgc aaggctgtga cgtggcgatc gacttcaccc atcccgatgt
     1081 ggcgttggag catatccgtt tttatatcga aaacggggtt cccgccgtga tcgggaccac
     1141 cgggtggtat gagcaccttg gtgaagtcgc ttcattggtc agggagggga acgggacgat
     1201 cctctacagc gggaattttt ctttgggggt cgcgcttttt ttacggagcg tacgatatgc
     1261 cgcggctctg tatggcaaga cgggacgcta cgatccggcg atcatcgaag tgcatcacaa
     1321 caaaaaagcg gacagccctt cgggaacggc gggcatgctc gcctcccggg tgctggaagg
     1381 ctttcctgaa aagacccgga tcgaatatga aacccagcac caaagacggg gggacgaggt
     1441 gctgcacgta tcaagccttc gggtcggttc ggtgccggga acccatacca tcctcttcga
     1501 cagcccggag gacacgatcg agctgatcca caccgcccga acccgggacg gcttcgcctt
     1561 cggcgcggtg gccgccgccg cgtggctgaa cgaacgtgaa agcgggctgt atacattgga
     1621 agagatgatt gacgcagtat ttctgcgata ggagacaatg aatgaagaca atcgatttca
     1681 ggggagtgca caccgcgctg gtcaccccgt tcaccgcaaa ggataatctg gatgaggacg
     1741 ccctgcaggc gatcatcgag catcagatcg aaagcggaat cagcggcctc gttccctgcg
     1801 ggacgaccgg cgagtcgccg accctcagtc acgacgagca cgaccgggtc atcgccctga
     1861 cggtgaagtt tgcaaacgga agggtgccgg tcatcgccgg aaccggttcg aacgcgaccg
     1921 atgaggcggt ccggctctcc cgccacgccc agcaggtcgg ttccgatgcg gtattgctgg
     1981 tcagccccta ttacaacaaa ccgacccaga aaggtctctt cgagcacttc aaggcgatcg
     2041 ccgacagcgt cgacattccc tgcatcctct acaacattca gggccgaacc gcggtgaaca
     2101 tcgagacggc gacggtggtg gcccttcagg aagcgtgccc caatatcgtc ggagtgaagg
     2161 aagcaagcgg aaagcttcag cagatgcagg aggtgatcga ctcctcgagg ccggagttct
     2221 tcgtcctcag cggggatgac aatatgtgtc tccccctgat cgagatcggc gggcacgggg
     2281 tgatcagcgt cgcttcgaat ctccttcccg gatatgtctc gacgatggtt gaaaaagcgc
     2341 tcaatggaga tatggccgaa gcgaaaaaga tggaagcggt catttcgaac ttcttccgca
     2401 cctgtttcct ggagaccaat ccgattccga tcaagacggc gatggcccga tacgggtggt
     2461 gcaacgaatc gttccgcctg ccgctctgca cctttgaaag cgaagccaag cgggcggagc
     2521 tctatcggga attgaaccac ttggaatcct tgaagatcat caagaggagg tagagatggc
     2581 gacgatcaac agcgcataca ggaacctggc aagcggatat ctctttcccg aaatcaatcg
     2641 gagagtccgc gcgtggcagg agcgaaatcc cgacaaacag gtgcttcggc tggggatcgg
     2701 aaacaccacc gaggcgcttc ctccggcaat ctgcaaagcg atgatcgaga aggtggaaga
     2761 gctggggacg cgggagacct acaccggcta cggagacgag cagggtgacg gatatctgcg
     2821 aaaggccttg gtcgaatact accaacaggg cggcgtttct ttggaaatgg atgaagtgtt
     2881 cgtcagcgac ggagcgaaaa gcgatgcggc gaatatccag accctctttt ccatcgattc
     2941 ggtcgttgct gtagccgatc ctgcgtatcc ggtctacgtg gattccaacg tggcaagcgg
     3001 ccgctcggcg gggtgggatg agagccgcgg aatgtacagg aaccttgtct acctctcctg
     3061 cacggaagaa aacggattca ttccgccgct tccctccgag catgtcgatc tgatctacct
     3121 gtgcaacccg aacaacccca ccggggcggt gatgaaccat gaacaggtga aacggttcgt
     3181 cgactatgcc cgaaaacaca agagcgtcat catctacgac agcgcctaca gcgaatacat
     3241 caccgaagag ggatatcccc gctcgatcta cgagatcaag ggggcgaagg agtgcgcgat
     3301 cgaaatctcc tcgttttcca agttctccgg attcaccggg gttcggctcg ggtggacgat
     3361 cgtccccaag aaccttgtct gcgaggacag caagcctctg gagctcaaca ggatgtggaa
     3421 ccgccgccag accacctttt tcaacggtgc gagcaatatc gcccagaggg gcggccatgc
     3481 ggcgctttcc ggggaagggt acgagcagag ccgggctctt gtgaagtact atctggaaaa
     3541 cgcccgcctg attcgggaag gtcttaagcg agtcggcctt accgtctacg gcggaatcaa
     3601 cagtccctac atctgggcga agaccccgga cggaatcgac agctgggcgt ttttcgatca
     3661 gctgctcgat cagtgccacg tcgtggtgac cccaggcggc ggcttcgggc cggcgggaaa
     3721 caggttcgtc cgggtatcgg cgtaca
//
LOCUS       DAOPUZ010000060         3724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_25090_length_3724_cov_2.792859, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000060 DAOPUZ010000000
VERSION     DAOPUZ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25090_length_3724_cov_2.792859"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKN79_01770"
     CDS             complement(<1..509)
                     /locus_tag="PKN79_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNZ94522.1"
                     /translation="MDTLKGRSYLTLRDYTEEEILYLLSLAKDLRSKKRGLAYPSHLK
                     GKLLDGKNIVLLFDKASTRTRCSFEVAAFDEGANVTYLTNSQMGKKESIEDTAKVLGR
                     LYDAIEYRGFGQSIVEDLARHSGVPVYNGLTDDDHPTQVLADVLTAVDHTGKQPHELV
                     FTYVGDGRN"
     gene            complement(644..1453)
                     /locus_tag="PKN79_01775"
     CDS             complement(644..1453)
                     /locus_tag="PKN79_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNZ94523.1"
                     /translation="MKKYLVAIFLIVLGASLLFAAGAKEVQDTKTILVGATPVPHAAF
                     LNLIVDDLAASGYTLKVREFTDYVTPNEALESGELDANFFQHIPYLETFNKDRGFHLV
                     NAGGIHVEPFALYSDRYASLEALPDGATIAIPNDPTNEGRALLLLESAGLLTLDSAAG
                     LEATPLDIVANPKNLRFREIEAASLPRVLKDVDGAIINGNYAIPAGLVATRDGLLVEG
                     ADSPYVNVVAVKAGRENEAGIKALVAALRSEKIKAYVAEHYKNGEVVLVTE"
     gene            complement(1508..2173)
                     /locus_tag="PKN79_01780"
     CDS             complement(1508..2173)
                     /locus_tag="PKN79_01780"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HNZ94524.1"
                     /translation="MSAKIWLLIWNSTLQTLSMVFFSTLFSLILGLPLGVLLFSTGEE
                     EQGGIIPMPLLNSVLSRIVNVLRSFPFIILMILLFPLSRLLIGTSIGTAATIVPLSIA
                     AAPFVARVIETALKEVDPGVVQAARAMGSTNFQIVRKVLIPEAMPSLVSGVALTIISL
                     IGYSAMAGAIGGGGLGDLAIRYGYQRFRGDIMLVAVIIILVLVEIVQVVGNRLSARLL
                     ARR"
     gene            complement(2170..3183)
                     /locus_tag="PKN79_01785"
     CDS             complement(2170..3183)
                     /locus_tag="PKN79_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ94525.1"
                     /translation="MQIRLKNLKRSYGSLHAVNGISLSIPSNLIYGIIGKSGAGKSTL
                     VRLISLLEKPDDGEVYFDELRVDNLAKEELIRRRRRVGMIFQNFNLFSSRNAAQNVAY
                     PLEIIGTPKGEIKARVAELLELVGLGDRGKAPISTLSGGQKQRVAIARALACNPDILF
                     CDEATSALDPQTTHSILALLKEIQRKMGLTIVMITHQMEVVRDVCDRVAVLDDGRVVE
                     EGPVTDIFAKPKSDVTKEFLSHLVGTDAGSEEQMVQWSSKRGAYILRFRGVTTDQPIL
                     SQITRETGIDFNIRAGGVQKVGGEVEIGTLIVDIQGSEDEQESVVARLRELGVHVEKE
                     ERS"
     gene            3319..>3724
                     /locus_tag="PKN79_01790"
     CDS             3319..>3724
                     /locus_tag="PKN79_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNZ94526.1"
                     /translation="MSGYRYETHLHTNQASACGLSTGRAYIRPYLDAGYSGIIVTDHF
                     FNGNTNIPHRLPWKERVRRLCAGYEEAKEYGDHLGLSVFFGFEYSYGQDEFLVYGLSP
                     EYLAEHPEMMGWDHRTLYETVDNEGGLVVQAHP"
BASE COUNT          830 a         1047 c         1013 g          834 t
ORIGIN      
        1 ttgttccggc catcaccgac ataggtgaag accagttcat gaggctgctt gcccgtgtgg
       61 tcgaccgccg tcagcacgtc ggcgagcacc tgggtcggat gatcatcatc cgttaacccg
      121 ttgtacacgg gaacaccgct gtggcgggca agatcttcga cgatcgattg tccgaagccg
      181 cggtactcga tcgcgtcgta gagccggccg agaaccttcg ccgtgtcctc gattgattcc
      241 ttctttccca tctggctgtt tgtcagatac gtcacgttcg ccccctcgtc aaaggcggcg
      301 acctcgaagg agcagcgggt ccgggtcgag gctttatcaa agagcagaac gatatttttg
      361 ccatccagaa gtttcccctt cagatgggag ggatatgcca gtcccctctt ctttgaacgg
      421 agatcctttg ccagcgagag gaggtagaga atctcctctt cagtgtaatc gcggagggtg
      481 aggtaactgc ggcctttgag agtatccata gcgcttctcc ttccggagag tattgcatat
      541 tcatgcaaat atcgtcaata aatatacaag tatttccatg catgcagatg cataaagaag
      601 acctccggca acacgccgga ggccagatct ttactacgac cggttattcg gttacaagaa
      661 cgacttcacc gtttttgtag tgttcggcga cgtatgcctt gatcttttcg ctgcggagcg
      721 ctgcgaccag agccttgatc ccggcttcgt tttcccggcc cgccttcacc gcgacgacgt
      781 tgacgtaggg actgtcggcg ccttcgacga gcaacccgtc acgagtggcc acgagacccg
      841 cgggaatcgc atagtttccg ttgatgatcg ccccgtcgac atccttcagc acgcggggaa
      901 ggctcgccgc ctcgatttca cggaagcgca ggttcttcgg attcgccacg atatcaagag
      961 gggtcgcttc caatcccgcc gcgctgtcga gcgtcaagag tccggcgctt tcaagaagca
     1021 ggagcgctcg gccttcgttg gtcgggtcat tgggaatagc gatcgtcgca ccgtcgggaa
     1081 gagcttccaa ggaagcatag cgatcgctgt agagggcgaa cggctcaacg tggatgccgc
     1141 cggcgttcac aaggtggaag ccgcgatcct tgttaaaggt ctccagatac gggatgtgct
     1201 ggaagaagtt cgcatcaagc tcgccgcttt caagcgcttc attgggggtc acgtaatcgg
     1261 tgaactcccg gactttgagc gtgtatccgc ttgccgccaa atcgtcgacg atcaggttca
     1321 ggaacgcggc gtggggcacg ggcgtcgcac cgacgagaat cgtcttggtg tcctgtacct
     1381 cttttgcgcc cgctgcgaag agaagcgatg cacccaaaac aatcagaaaa atcgctacca
     1441 aatacttttt catactatac tcctatttcc catcggggat aattcatgtt cttgataata
     1501 ttgtttctca gcgtcgggcc aagagccggg cgctgagccg gttgcccact acctgaacga
     1561 tctcaacgag aacgaggata atgattaccg caaccagcat gatgtcgccg cggaagcgct
     1621 gatacccgta acggatcgcc aaatccccca accctccgcc gccgatcgct ccggccatcg
     1681 cagagtaacc gatcaggctg atgatcgtga gggcgactcc gctgaccaga gagggcatcg
     1741 cctcgggaat caacaccttc cggacgatct ggaagttcgt cgatcccatt gcacgggcgg
     1801 cttgaacgac gcccggatcc acctccttga gtgcggtctc gatgacgcgc gcgacgaacg
     1861 gagctgccgc gatcgacagc ggaacgatcg tagcggcggt tccgatactc gttccgatca
     1921 acaacctcga aagagggaac aacagaatca tgagaatgat gaaggggaag gagcgcagca
     1981 cgttcacgat tcggctcagc accgaattca acaaaggcat gggtatgatg ccgccctgct
     2041 cttcttcacc ggtgctgaag agcagcactc ccaaggggag ccccaatatc agggagaaca
     2101 gggtggagaa aaacaccatg ctgagggtct ggagggttga gttccaaatc aagagccaga
     2161 tcttcgcact catgagcgtt cctccttttc gacatggact ccaagctcgc gcagccttgc
     2221 aaccacgctt tcctgctcat cctcgcttcc ctggatatcg acgatcaacg ttccgatttc
     2281 aacctctccg ccgaccttct gaactccgcc tgcccggatg ttgaaatcga ttcccgtctc
     2341 gcgggtaatc tgactgagta tcggttgatc ggtggtaact ccgcggaagc gcaggatgta
     2401 ggctccccgc ttgctggacc attggaccat ctgctcttca ctgcccgcat ccgttcccac
     2461 caagtgggag aggaactctt tggtgacatc gcttttaggc ttggcgaaga tgtccgttac
     2521 cggcccttct tcgacgaccc taccgtcatc gagaaccgcc acgcggtcgc acacatcccg
     2581 caccacttcc atctggtggg tgatcatcac gatcgttaag cccatcttgc gttgaatctc
     2641 tttcaacaac gcgaggatcg aatgggtggt ttggggatcc agggcgctgg tcgcttcatc
     2701 gcagaagagg atgtcgggat tgcacgccag agcccgggcg atagccactc gttgcttctg
     2761 cccgccgctg agggtggaga tcggagcttt tccgcgatcg ccaagaccga caagttcaag
     2821 cagctcggcg acccgagcct tgatctcccc tttcggggtc ccgatgattt caaggggata
     2881 ggctacgttc tgggcggcgt ttcgactgga aaagaggttg aagttctgga aaatcatccc
     2941 cactcgcctg cggcgccgga tgagctcctc cttggccagg ttatcgaccc tcagttcatc
     3001 gaaatacact tcaccgtcat ccggtttttc cagaagactg atcaggcgca ccaaagtgga
     3061 tttgcccgct ccgcttttac cgatgattcc gtagatcaga ttggaaggga tggacagact
     3121 gatcccgttg accgcatgaa gcgatccata actccttttc aagtttttca gacgaatctg
     3181 catatatata ccttccatat tcataaaacg gattgagacc atcatagagg aaaggtggtt
     3241 cttgaacaag tagagcaagg atatccatct tggagaaggt tttggaaaag gatatagttg
     3301 gatgaaaaag gaatacttat gagtggatac cgctacgaga cccatcttca taccaatcag
     3361 gccagcgcat gcggtctctc caccggaaga gcatatatcc ggccatacct tgatgccgga
     3421 tacagcggca tcatcgtaac cgaccacttc ttcaacggaa acacaaatat tccccatagg
     3481 ctaccttgga aggagcgggt tcgcaggctg tgcgcgggat atgaagaagc caaagagtac
     3541 ggcgatcatc tgggtctttc cgtcttcttc ggttttgaat actcatacgg gcaggacgag
     3601 tttctcgtct atggcctttc gccggagtat ctggccgagc atcccgagat gatgggttgg
     3661 gatcatcgca ccctctatga gacggtcgac aacgaagggg ggctcgtcgt tcaagcccac
     3721 ccgt
//
LOCUS       DAOPUZ010000061         3700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_25325_length_3700_cov_3.072977, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000061 DAOPUZ010000000
VERSION     DAOPUZ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25325_length_3700_cov_3.072977"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKN79_01795"
     CDS             <1..389
                     /locus_tag="PKN79_01795"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNZ94527.1"
                     /translation="APYVQRLIEMAEHLSPEDFIATATAFTAWSIADSYKRFVMPRHG
                     LDRVVVSGGGARNKTLLSYLESYLDREIVTGEAYGVPDQGREAMAFALLAHESLNRRP
                     SNFPLATGARERTILGTITLQEPVQI"
     gene            complement(390..1544)
                     /locus_tag="PKN79_01800"
     CDS             complement(390..1544)
                     /locus_tag="PKN79_01800"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNZ94528.1"
                     /translation="MKKRLLVVHGGAPTAVINASLYGVIKEAEGRYGKIYGAKGGSGG
                     ILKEAFVDLLSLPKEAIERLPHTPGSMIGTSRDHIDAASYRRIVDILIANEIDTVLFN
                     GGNGSMDTAGKIAREIQKRNRPIRIIGIPKTIDNDIAVTDHAPGFGSAARYLAASVQE
                     LAADVAALPIHVSVIESMGRNTGWLTAAALIARREGIYGPHLLYAPELAFDEERFLDE
                     VSSLYKKHRHVVVVASEGLRTANGDPIVEPIFKTGRSVYYGDVSAHLAMLVIKRLGVK
                     ARSEKPGILGRSSIAFQSSVDRDEAILVGRKAVQAALDGNSEVMVGFKRISNEPYRVE
                     PVLIPLEEVMLHEKTLPMEYLPSFGTVDESYLSWCKPLIGGPLADFSPVL"
     gene            complement(1541..2692)
                     /gene="nagA"
                     /locus_tag="PKN79_01805"
     CDS             complement(1541..2692)
                     /gene="nagA"
                     /locus_tag="PKN79_01805"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNZ94529.1"
                     /translation="MNESLLIRGGKVYRNGRCEELDLFIEDGAFAGFDRDRPAGEVFE
                     AETMHVIPGMIDIHTHGAVGVDFNLFADEREIEKVVGFFASKGVSGFLPTVLTDEPEV
                     MKTQLSKLTRPALLKRFPQILGIHLEGPFLNASYKGAMKEELLKECDLSCFDELQDAA
                     RGMIRVITLSPEVRGCGILIRELTGRGVRVSLGHSGAGYEETIGAIDAGASSTTHIMN
                     AMKLLHMHDPAILTAVLERDIYAEMIVDGFHLHPPIVRLLLKIKGWDRMIAVTDSIMA
                     AGYPDGEYLLGPNRVIVKDGDAKLVENGVRAGSTLTMDKALRNLLDFTRAPLQRIVPL
                     LSTNAARMLGLSDLGEIALGKRADATILDSSMDVVATITSGKIAYRREE"
     gene            complement(2685..>3700)
                     /locus_tag="PKN79_01810"
     CDS             complement(2685..>3700)
                     /locus_tag="PKN79_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ94530.1"
                     /translation="DIAKETGLSISTVSRILSNDPNRSFSDEAIAKVQEAAGRLGYFE
                     KYRMRSKALTTMQLSIACILTSDHETYLSPFFAALLEGVQEEVFRQSSRNALTFHTLY
                     VKDPSFPAFLETTNLDGAIILGRTTIDTIQRLKRSIPTLLYAGVNRLGNGVDEVICDA
                     ESATKEAVEHLLALGHRAIGFIGHIPQKDETINEHRFNGYLGALRKAGIEYDDSLVVN
                     TILTATGGYQATKTLISRGKIPTAIFCSNDTVAMGVLKALDEAGIHVPNDLSLIGFDN
                     IEATAFTKPALTTIDIPKRDLGRLAVKTLLDRIESKRTFPISVRLPFTLIRRESCKEV
                     ADE"
BASE COUNT          792 a         1091 c          995 g          822 t
ORIGIN      
        1 gcgctcccta cgttcagcgc ctgatcgaaa tggcggaaca cctctcgccc gaagacttca
       61 tcgctacggc caccgcattt accgcctggt cgatcgccga cagctacaag cgcttcgtga
      121 tgcctcggca cggccttgac cgggtggttg tcagtggcgg cggagcccgg aacaagacgc
      181 ttctttccta ccttgaatcc tatctcgacc gtgaaatagt gaccggcgaa gcgtatggtg
      241 ttcccgacca gggacgggag gcgatggcgt tcgcccttct tgcccatgag tcattgaaca
      301 ggcgaccgtc caacttcccg ctcgccaccg gtgcccggga aaggacgatc ctcgggacga
      361 tcaccctgca ggagccggtt caaatctgat cacagcaccg gactgaagtc ggcgagcggg
      421 cctccgatga gcggcttgca ccaggaaaga tatgattcat cgacggttcc gaaggaaggc
      481 agatattcca tcggaagggt cttttcgtgg agcatcacct cttcaagcgg gataagaaca
      541 ggctcgacgc gatagggttc attactgatt cgcttaaagc cgaccatcac ttcgctgttg
      601 ccgtccaagg ccgcctgaac ggctttcctg ccgacgagaa tcgcttcgtc gcgatcgacg
      661 gagctttgaa aggcgatcga ggaacgcccg agaattcccg gtttttcact tcgggctttg
      721 actccaaggc gtttgatcac cagcatcgca aggtgggcgc tcacatcgcc gtagtacact
      781 gaacggccgg tcttgaagat cggctcgacg atcggatcgc cattcgcagt gcgcagtccc
      841 tcgctggcca cgacgacgac gtgccgatgt tttttataga gggaggagac ttcatccagg
      901 aagcgctctt cgtcaaaagc gagttcgggg gcatacagga gatgggggcc gtagatgcct
      961 tcgcgccttg cgataagcgc ggcggcggtc agccaacccg tattccggcc catcgattcg
     1021 atcaccgaga catgaatcgg cagagccgcc acatcggcgg caagctcctg aacggaagca
     1081 gcgagatagc gagcggcgct tccaaagccg ggagcatggt cggtgacggc gatatcgttg
     1141 tcgatcgttt tgggaatgcc gattatcctg atcggacgat ttcgcttttg gatctcccga
     1201 gcgatttttc ccgcggtatc catcgacccg ttccctccat tgaagagcac ggtatctatt
     1261 tcattggcga tgagaatgtc gacgatcctc cgataggaag cggcatcgat gtgatcgcgg
     1321 ctggttccga tcatcgagcc cggagtatga gggagacgtt caatcgcttc cttcggcagg
     1381 gaaagaaggt cgacgaatgc ttccttcagg attccccccg aaccgccttt ggctccgtag
     1441 atcttcccgt accgaccttc agcttcttta atgacgccgt acagcgaagc attgatgacc
     1501 gcagtggggg ctccgccatg aacaaccaaa agacgtttct tcattcttct ctccgataag
     1561 ctattttgcc tgaagtgatg gtggcgacca catccattga tgaatccaag atcgtcgcat
     1621 cagcccgttt tccaagggcg atctccccca agtccgacaa tccgagcatt cgggcggcgt
     1681 tggtggaaag aagcgggacg atccgctgaa gcggggcccg ggtaaaatca agaagatttc
     1741 tcagcgcttt gtccatcgtc agcgtcgaac cggcccggac gccgttctcg acaagtttcg
     1801 catccccgtc cttgacgatg acccggttgg ggccaaggag atactcgccg tcgggatacc
     1861 ccgccgccat gatcgaatcc gtaacggcga tcatccgatc ccaccctttt atcttgagca
     1921 acagtcggac gatcggtgga tggaggtgga agccgtcaac gatcatctcc gcatagatgt
     1981 cacgctccag aaccgcggtg aggatcgccg gatcgtgcat gtggagcagc ttcatcgcat
     2041 tcatgatgtg ggtagtggaa ctcgctccgg cgtcgatcgc tccaatcgtc tcctcgtagc
     2101 cggctccgct gtgcccgagg ctgactcgga ctccccgtcc ggtcaactcc ctgatcaaga
     2161 tcccgcaacc ccgtacctca ggggaaaggg tgatcactct gatcatcccg cgagctgcat
     2221 cctgcagctc gtcaaaacag gaaaggtcgc actccttcag gagctcttcc ttcatcgctc
     2281 ccttatagga ggcgttgaga aacggtcctt ccagatgaat cccgagaatc tggggaaagc
     2341 gtttgagcaa tgccggtcgg gtaagcttgg aaagctgggt tttcatcacc tcgggctcat
     2401 cggtgagaac cgtcggcaga aacccgctca cccctttgga agcaaagaaa ccgacaacct
     2461 tctctatctc ccgttcatcg gcaaaaaggt tgaaatccac ccccaccgcc ccgtgggtat
     2521 ggatatcgat cattccgggg atcacatgca tagtttccgc ttcaaacacc tctccggcgg
     2581 gacgatcccg atcgaagccg gcgaatgctc catcctcaat gaacaaatcc agctcttcac
     2641 atcttccatt ccgatagacc tttccgcctc tgatcagcaa cgactcattc atcggccacc
     2701 tctttgcagc tctcccgccg aatcagcgtg aatgggagcc ggacgctgat cgggaatgtc
     2761 cgcttgcttt caatccgatc caaaagagtc ttgacggcaa gccttccgag atcccgcttg
     2821 ggaatatcga tcgtcgtcaa cgcgggcttg gtgaacgccg tcgcttcgat gttgtcgaaa
     2881 ccgatgagcg acagatcgtt cggaacgtgg atacccgctt catcaagcgc tttcagcacc
     2941 cccatcgcca cggtgtcatt gctacagaag atcgcggtgg gaatctttcc ccggctgatg
     3001 agcgttttcg tcgcttgata gcctcctgta gcggtcaaaa tcgtattgac gaccagtgaa
     3061 tcgtcatatt cgatccccgc cttccgcaaa gctcccagat atccattaaa ccggtgctcg
     3121 ttgatcgtct catctttttg cgggatgtgc ccgatgaacc cgatcgcccg gtggccgagt
     3181 gcaagcagat gctccaccgc ttcttttgtg gcgctttccg catcgcagat cacttcatcc
     3241 actccatttc ccaaacggtt cactcccgca tagagcaagg tgggaatgct ccgtttcagc
     3301 ctttgaatcg tatcgatcgt cgttcgtccg agaataatcg ccccgtcgag gttggtcgtt
     3361 tccaaaaaag cgggaaagct cgggtcctta acgtacaggg tatggaacgt cagtgcgttg
     3421 cgggaactct ggcggaacac ctcttcctga acaccctcca gaagcgccgc aaagaacggg
     3481 gaaagatagg tttcatgatc gctggtgagg atgcaggcga tggacagctg catcgtcgtc
     3541 aaggctttcg aacgcatccg atatttctca aaatacccta atcgacccgc tgcttcctgg
     3601 actttggcga tcgcctcgtc actgaatgat cgattggggt catttgagag aatccgggaa
     3661 accgtcgaga tcgacagccc tgtctctttc gcgatatctt
//
LOCUS       DAOPUZ010000062         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_25348_length_3697_cov_3.184514, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000062 DAOPUZ010000000
VERSION     DAOPUZ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25348_length_3697_cov_3.184514"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /gene="rpsD"
                     /locus_tag="PKN79_01815"
     CDS             complement(<1..516)
                     /gene="rpsD"
                     /locus_tag="PKN79_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNZ94531.1"
                     /translation="MARYTGPKCRYCRAEGTKLFLKGNRCLSGKCPLSSSGKRTGLPG
                     KDPRARSKKPTNYGLQLREKQKLKRTYAMLEKQFKLTFNEAARIPGKTGENLIQLLEQ
                     RLDNVVFRLHFASSRYQAAQFVNHGHIFVNGKRVDIPSYRVKVGDEISISPQAQKMLI
                     IKENLKEYTKSG"
     gene            complement(534..911)
                     /gene="rpsK"
                     /locus_tag="PKN79_01820"
     CDS             complement(534..911)
                     /gene="rpsK"
                     /locus_tag="PKN79_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNZ94532.1"
                     /translation="MATVKRKTKKTVFEGNVYIQATFNNTIVTVTDLQGNAVSWASAG
                     GLGFRGAKKSTPYAAQTTAEKAAKTAMDSGLQEVNVFVKGPGVGRESAIRTLGVLGLK
                     VRSIRDITPIPHNGCRPRKSRRV"
     gene            complement(926..1291)
                     /gene="rpsM"
                     /locus_tag="PKN79_01825"
     CDS             complement(926..1291)
                     /gene="rpsM"
                     /locus_tag="PKN79_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNZ94533.1"
                     /translation="MARIAGVDLPNKATKIALTYIYGIGRYSAIEICEKTNIDPDINI
                     NTLSNDDLAVLRKVIEEEYKVEGRLRTEVALNIKRLMDIGCYRGLRHRRGLPVNGQRT
                     KTNARTRKGRKKTVAGKKK"
     gene            complement(1330..1443)
                     /gene="rpmJ"
                     /locus_tag="PKN79_01830"
     CDS             complement(1330..1443)
                     /gene="rpmJ"
                     /locus_tag="PKN79_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNZ94534.1"
                     /translation="MKVRSSVKPICDKCKVVRRSGVVRIICENPKHKQKQN"
     gene            complement(1461..2780)
                     /gene="secY"
                     /locus_tag="PKN79_01835"
     CDS             complement(1461..2780)
                     /gene="secY"
                     /locus_tag="PKN79_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNZ94535.1"
                     /translation="MANTLVEMYRIKSLRKKIFITLGLLVVSRIGAVIPIPGIDPQVL
                     KLFFLAQSSSSTIGLTEYLNFFSGGAFSNFSLFMLGVMPYISTQIILQLLMLVIPSLK
                     KLASDPSGHKKLQQYTRYGTVVVCLLQSYVVTIYAQSIPGVLTMGLGSFTLIAMLTVT
                     TGSMLLIWIGNKITQWGIGNGISLLIFAGIVARFPTAVNALFEAIKGGVLNPIVVLIV
                     AVMFLIVVALVVYEEQGIRKIPVNYAKRVVGRKMYGAQSTYIPIKVNPSGVIPVIFAS
                     ALLSFPLQIATTLGPEVRWLAAFANWLNPQGTPYLIIYALLIVAFAFFYTQVSMNPVE
                     MAKQIRENGGSVPGVRAEKLEEYLTKVLNRIVLPGSLFLAFIALIPTLVQKFFNFPST
                     VAMLFGGTSLLILVGVELDTIRQIEGIMKMHHYDGFKIGQKKKTKHF"
     gene            complement(2792..3223)
                     /gene="rplO"
                     /locus_tag="PKN79_01840"
     CDS             complement(2792..3223)
                     /gene="rplO"
                     /locus_tag="PKN79_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNZ94536.1"
                     /translation="MAKIIAPKGANTKKTILGRGASSKGRTSGRGHDGQNSRSGGGTR
                     PGFEGGQMPLYRRVARRGFSNEPFKVVYEVVSLDDISRVFNDGDVVTLAALKELGVVK
                     GVKTQAKILNNGELTKKVIIEGLKVSASAVEAIKAAGGQIR"
     gene            complement(3225..3422)
                     /gene="rpmD"
                     /locus_tag="PKN79_01845"
     CDS             complement(3225..3422)
                     /gene="rpmD"
                     /locus_tag="PKN79_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNZ94537.1"
                     /translation="MAKAKKQKQIKITLVRGLAGTLPKQRRTVKALGLGRISSSVVHD
                     ANPVILGMVNVVSHLVKVEEM"
     gene            complement(3426..>3697)
                     /locus_tag="PKN79_01850"
     CDS             complement(3426..>3697)
                     /locus_tag="PKN79_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNZ94538.1"
                     /translation="NSTIPHEIIGKYKGASVLLKPAVPGTGVIAGGTVRAICDAAGIH
                     DILSKSLGSKNHINTVKATFVALENLFNVREIAKNRGKSLSEMWG"
BASE COUNT          782 a         1047 c          941 g          927 t
ORIGIN      
        1 tccgcttttg gtgtactcct tgaggttctc cttgatgatg agcatcttct gagcctgcgg
       61 gctgatcgag atctcatcac ccaccttgac gcggtaggaa ggaatatcga cgcgcttgcc
      121 gtttacgaag atgtgtccgt ggttcacgaa ctgagcagcc tgataacgac ttgaagcgaa
      181 gtgaaggcgg aaaacgacgt tgtccaagcg ttgctccaag agttggatca agttctcacc
      241 tgtctttccg ggaattcttg ccgcctcgtt gaaggtgagc ttgaactgct tctcaagcat
      301 cgcatacgtt ctcttgagct tctgcttctc acgaagctga aggccgtagt tggtcggctt
      361 cttcgatcgg gctcggggat ccttgccggg aaggccggtg cgctttccac tgctgctcag
      421 cgggcacttg ccggaaagac agcgattgcc cttgaggaaa agcttggtgc cttccgccct
      481 gcagtatcta catttaggtc cggtatatct tgccatctgg ttctatctcc tcatcacact
      541 cttctactct tgcgcggtcg gcacccgttg tggggaatcg gggtgatgtc gcgaatcgat
      601 cgcactttca acccgagtac accaagggtt cgaatggcgc tttcacgacc gaccccggga
      661 cctttgacaa atacgttcac ttcctgaagt ccactgtcca tggcggtctt tgccgctttc
      721 tcagcagtgg tctgcgcagc atacggagtg gatttctttg ctccgcggaa cccgagtccg
      781 ccggcactcg cccacgagac ggcgttgcct tgcagatcgg ttacggtgac gatcgtgttg
      841 ttgaaggtag cctgaatata gacgtttcct tcgaataccg tcttttttgt cttacgtttt
      901 acagttgcca tctgtgatat gctccttact tcttcttacc ggcgacggtc ttcttcctgc
      961 ccttgcgggt tcgggcgttc gtcttcgtcc gctggccgtt gaccggcaag ccccttcggt
     1021 gtctgaggcc gcgatagcag ccgatatcca taaggcgctt gatattcagg gctacctcgg
     1081 ttcgaaggcg tccctctact ttgtactctt cttcgatcac ctttcgcagt actgcaaggt
     1141 catcgttgga aagggtgttg atattgatat ccggatcgat atttgtcttt tcacaaatct
     1201 cgatcgccga atacctaccg attccgtaga tataggtcag ggcaattttc gttgccttgt
     1261 tcggcaaatc tacacctgca attctcgcca tccatggcct cctgaaattc tcacagggtt
     1321 gctaccctac taattttgtt tctgcttgtg cttggggttc tcgcagatga tccgaaccac
     1381 cccactccgt cttactacct tgcatttatc acaaataggt ttgacactcg atcttacttt
     1441 catcttccag ctcctcgtgc ttagaaatgc ttcgttttct tcttctgccc gattttaaat
     1501 ccatcgtagt ggtgcatctt catgatgccc tcgatctggc ggatcgtatc aagctcaact
     1561 ccgaccagga tcagcagcga agtacctccg aagagcatcg cgaccgtcga ggggaagttg
     1621 aagaacttct gtacgagcgt cggaatcaag gcgatgaacg ccaggaacaa cgaaccgggc
     1681 agaacaatcc ggttaagaac cttggttaag tactcctcaa gcttctccgc ccgaaccccg
     1741 ggaacggatc caccgttctc ccggatctgc ttggccattt cgaccgggtt catcgacacc
     1801 tgggtgtaga agaacgcgaa cgctacgatc aacagggcgt agatgatgag atagggggtg
     1861 ccctgaggat ttaaccaatt ggcgaatgcg gcaagccagc ggacttccgg ccccaaggtc
     1921 gttgcaatct gcaaggggaa ggagagcagc gcactggcga aaatgaccgg aatgacgccc
     1981 gaggggttca ccttgatggg gatgtacgtt gattgcgcac cgtacatctt tcggcccacc
     2041 acgcgcttgg cgtagtttac cggaatcttt cggattccct gctcctcgta cacgacgagg
     2101 gcgactacga tgaggaacat caccgcaacg atcagcacga cgatcgggtt cagcaccccg
     2161 ccctttatcg cttcgaacaa agcgttcacg gcggtcggga agcgggcgac gataccggca
     2221 aaaatcaaga ggctgatacc gtttccgatt ccccactggg tgatcttgtt accgatccaa
     2281 atcagcagca tcgaaccggt ggttacggta agcatggcaa tcagggtaaa gcttcccaag
     2341 cccatcgtca gcacgccggg gatcgactgt gcgtaaatcg tcactacata tgattgaagc
     2401 agacatacca ctacggttcc atagcgggtg tactgctgca acttcttgtg ccccgaagga
     2461 tcggaggcga gcttcttcaa cgagggaatc accagcatca aaagttgcag aatgatctgc
     2521 gtactgatgt acggcataac cccgagcatg aagagcgaga agttggaaaa cgctccgccg
     2581 ctgaagaagt tcaaatactc ggtcaggccg atcgtcgaac tggaactctg ggcaaggaag
     2641 aacagcttca acacttgcgg atcgattccg ggaatcggaa tcacggcccc gattctgctg
     2701 acaaccagca atccgagggt tatgaaaatc ttcttgcgaa gactcttgat tctatacatc
     2761 tcaactaagg tgtttgccat gacgcccttc tttatctgat ctggccgccc gcagccttga
     2821 tcgcttcgac cgcgcttgcg gaaaccttca atccttcaat gataaccttc ttggtaagct
     2881 ccccgttgtt gaggatcttc gcctgagtct tcacgccctt caccactcca agctccttca
     2941 gggctgcgag ggtgaccaca tctccatcat tgaacactcg ggagatatcg tctaaggaaa
     3001 cgacctcata caccaccttg aacggctcgt tggagaaacc tcgacgagcg acgcggcgat
     3061 agagcggcat ctgtccgccc tcgaagccgg ggcgcgtgcc tccgccggag cgggagttct
     3121 gcccgtcgtg tccccttccg ctggttcgac ccttggaaga agctccccga ccgagaatcg
     3181 ttttcttggt attggcaccc ttcggtgcga taatctttgc catcttacat ctcctcgact
     3241 tttacgaggt gcgaaacgac gttcaccatt cccaggataa ccgggtttgc atcatgcacc
     3301 accgagctgg aaatacgccc caagccgagg gccttcacgg tccgtcgttg cttgggaagc
     3361 gttcccgcaa gccctctgac gagggtaatc ttaatctgct tctgcttttt tgccttggcc
     3421 atctcttacc cccacatctc actcagggat ttgccccggt tcttggcaat ctcacggaca
     3481 ttgaaaagat tctccaacgc aacgaacgtt gccttcacgg tattgatatg gttcttggag
     3541 ccaagcgact tgctgaggat atcgtgaatt cctgccgcat cgcagatcgc tcgaaccgta
     3601 ccgccggcga tgactccggt accgggaacc gccggcttca gcagaacgct ggcgcctttg
     3661 tactttccga tgatctcatg cggaatcgtg gagttct
//
LOCUS       DAOPUZ010000063         3687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_25438_length_3687_cov_2.595540, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000063 DAOPUZ010000000
VERSION     DAOPUZ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25438_length_3687_cov_2.595540"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PKN79_01855"
     CDS             <1..600
                     /locus_tag="PKN79_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ94539.1"
                     /translation="EMIPKEDWPTSLEDLTHPRFRKNIILIDPRTSNVGLGLLLWTIH
                     IFGEEGWLDWWKAMKDQVLTITDGWSGAYGLFIEGEAPIVLSYTTSPVYHYTYESSTR
                     YQALIFDEGHQATIEGVGMLRSSKRKEEAKLFIDFLLSEAQLPIALANSMYPTNMNTA
                     LTEGFAYAPKPGVSLRMESEVIEKNLDRWLHEWTEVMSR"
     gene            597..2264
                     /locus_tag="PKN79_01860"
     CDS             597..2264
                     /locus_tag="PKN79_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNZ94540.1"
                     /translation="MSDYRRFYRQKSIGPLVILFSLFALPLALTIQSGVWEEGSFTLE
                     RIVSAILDPYTQRILLFTLKQAFYSTGAALLIGLPGAYILATYSFRGKKLIKALATIP
                     FVLPSILVVLGFVMFFGNSGTLNTVLMKVFDLDDPPLKLLYSLGAIILAHTFYNFPII
                     MSIVATAWEGLDTKLEAAAATLGSSPRKVFRTITLPRLVPSIISAAALVFLFCFNSFA
                     VILVLGGGPQFTTLEVEIYRQARMMGDPSSASALAIVSMLINGILFLGYAFSQRTLTV
                     TETVAERAFEERKPSPMRTYFILLYSLFTIIFLLGPIISVLYRSFSKGASRASETTFS
                     TTWYRQLLGFEQSSSHMGSALTALINSTLIGVAVSILTVTLALLLSVSIARRKKNSSF
                     LELFGMLPMMTSSVIVGLGYVLIARLLRPSLFGTYLLITLAHTVIAFPFALRTILPSV
                     RSLSETYLHASYTLGFSPQKTFRTVEIPLLRRTLLTAAVFAFALSLGEFNATLILSSS
                     RIVTLPVVMYRLIGSYHFQGACALGSILIITSGLAFFLGEVAKGDSV"
     gene            2261..3295
                     /locus_tag="PKN79_01865"
     CDS             2261..3295
                     /locus_tag="PKN79_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ94541.1"
                     /translation="MILQCSLQATYPDFTLDLAFTVQEGDLVCIIGPSGCGKSTTLSM
                     IAGLIHAETGSIILGGEDISQKEVHKRKIAMVFQDYSLFPHMNVEQNIAYPLKNLKLK
                     KQQRVQAVKELLELVNLQGYEKRKSTRLSGGERQRVALARALASEPKILLLDEPLSAL
                     DAKLRTRLREEIRRIHDETGITTIYVTHDQDEALAIADQVIVMDQGRIIQQGTPEEIY
                     RTPRTKSVATFTGEGTILPASAFARTLRNPYHLEIPAKHRDPKNTSLFFRPHASHIVE
                     DPGEVRLQYLPFLAFDEVRVIRSEFQGTHWRNSCLWNEHELLCHSERKPKKEIVSLHV
                     DITDLSYFEE"
     gene            3356..>3687
                     /locus_tag="PKN79_01870"
     CDS             3356..>3687
                     /locus_tag="PKN79_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94542.1"
                     /translation="MKRPLVLSLLLLFALPIAGGNFVTTVAYVPSAYLSIPHFEDELP
                     FRTSMSNTFIFEPFGYEADTWSVNASLKGAFVTPSLPWGSYRARGFTSLGLGARFSYS
                     LTDRFALFG"
BASE COUNT          783 a         1110 c          910 g          884 t
ORIGIN      
        1 gagatgattc ccaaagaaga ttggccgaca tcattggaag atttgactca tcctcgtttc
       61 aggaagaaca tcatcctgat cgatccgagg acctccaatg tcggtctcgg gttgctcctg
      121 tggacgattc acatcttcgg ggaagaaggc tggctcgatt ggtggaaagc gatgaaggac
      181 caagtgctta ccatcaccga cgggtggtcc ggcgcctatg gactcttcat tgaaggagag
      241 gctcccatcg tcttaagcta taccacgagt ccggtctatc actacaccta tgaaagctcc
      301 acccgctatc aagccctcat ctttgatgaa ggacaccagg caacgattga gggggtgggc
      361 atgctccgct cctcaaaacg aaaggaggaa gcgaagctgt tcatcgattt tctcttaagc
      421 gaagcgcagc ttccgattgc gttggcgaac tcaatgtatc cgaccaatat gaacacagct
      481 ctcaccgaag ggttcgccta tgctcccaag ccgggggttt cgctccgaat ggagagtgaa
      541 gtgattgaaa aaaacctcga tcgatggttg catgaatgga cggaggtcat gagtcgatga
      601 gtgattatcg gcgattctat cgccagaaga gcatcggacc gctagtcatc ctcttctcgc
      661 tgttcgccct tcccctcgcc ctgacgattc agtcaggggt gtgggaagaa ggctccttca
      721 ccttggagcg gatcgtttcg gcgatcttgg atccctacac gcagcgaatc cttctcttca
      781 ccctgaagca ggcgttctat tcgaccggcg ctgctctgct catcgggttg ccgggagcgt
      841 atattctggc gacctactcc tttcgaggca agaagctgat caaggcgttg gcgacgattc
      901 ccttcgttct tccatcaatt ctcgtagtct tgggatttgt catgttcttt ggaaacagcg
      961 gaacgttgaa tacggttctg atgaaggtct ttgacctcga cgatcctccg ctgaagcttc
     1021 tgtactccct tggagcgatt attcttgctc acacgtttta caacttcccg atcatcatga
     1081 gcatcgtcgc aaccgcctgg gaaggccttg acaccaaact tgaagctgca gcggcgacct
     1141 tgggatcatc tccccgtaaa gtgttccgaa cgatcaccct gcctcggctc gtcccatcga
     1201 tcatcagcgc tgcggccttg gtctttctct tctgtttcaa ctcgtttgca gtcatcctcg
     1261 tcctcggagg cggacctcaa ttcaccacac tggaagttga aatctaccgc caagcccgca
     1321 tgatgggcga cccttcaagc gccagcgcct tggcgattgt ctcgatgctg atcaacggaa
     1381 tcctcttctt gggatacgct ttcagccagc ggactcttac ggttaccgaa acggttgccg
     1441 agcgagcgtt tgaggagcga aaaccttcgc cgatgagaac ctatttcatc ctcctctact
     1501 ccctcttcac gatcatcttc ttactcggtc cgattatctc ggttctctat cgatccttct
     1561 caaaaggagc ttcacgcgca agcgagacga ccttcagcac tacgtggtat cgacagctcc
     1621 ttggctttga gcaatcctcc agtcacatgg gcagtgcctt gaccgccttg atcaattcga
     1681 ccctcatcgg agtagcggtg agtatcctca ccgtgacgtt ggctctcctg ttgagtgttt
     1741 cgatcgcccg tcggaaaaag aactcatcct ttttggagct cttcggaatg cttccgatga
     1801 tgaccagcag tgtcatcgtc gggctggggt acgtgttgat cgcccgcctt cttcgaccgt
     1861 cgcttttcgg aacgtatctg ctcatcaccc ttgcccacac cgtcatcgcc tttccgttcg
     1921 ccctcagaac cattctcccg agcgtccgtt ccctctcgga aacatatctg cacgcttcct
     1981 ataccttggg cttctcacct caaaaaacgt tccgtaccgt cgaaatcccg cttttgagaa
     2041 gaacgctgct cacggcggcg gtcttcgcct tcgccctgtc actcggtgaa ttcaatgcga
     2101 ccctcatcct ctcaagcagc cggatcgtga ccctgccggt ggtcatgtac cgcctcatcg
     2161 gctcgtatca tttccaaggc gcctgcgcct taggatcgat cctcatcatc accagcggcc
     2221 ttgccttctt ccttggtgaa gttgccaaag gagattccgt atgatcctcc aatgttccct
     2281 tcaagccacc tatccggatt tcacccttga tctcgccttc accgttcagg aaggtgatct
     2341 ggtgtgcatc atcggtcctt caggatgcgg caaatccacc accctgagca tgatcgccgg
     2401 cctgatccac gccgagaccg gatcgatcat tctcggtggc gaggatatca gccaaaaaga
     2461 ggtgcacaag cgaaagatcg ctatggtctt tcaggattac tcccttttcc cccatatgaa
     2521 tgtggaacaa aacatcgcct atcccctgaa aaacctgaag ctgaagaagc agcaacgagt
     2581 tcaggcggtc aaggaactcc tagagctggt gaatcttcag ggatatgaga aacggaaaag
     2641 cacccgcttg agcggcggcg aacgtcagcg cgtcgccttg gcacgggcgt tggcctcgga
     2701 accgaaaata ctgcttctcg atgaaccgct ttccgccctt gatgcaaagc tgcgaacccg
     2761 cttgagggaa gaaatccgcc ggatccacga tgagacgggc attaccacga tctacgtcac
     2821 acacgaccag gatgaagcgc ttgcgatcgc cgatcaggtg atcgtgatgg atcaggggcg
     2881 aatcatccag caggggaccc ccgaggagat ctatcgaacc cccaggacga agtctgtcgc
     2941 cacgttcacc ggggaaggaa cgatccttcc cgccagtgcg ttcgcccgga cgctgaggaa
     3001 cccctaccac cttgagatcc cggccaagca ccgggatccg aagaacacct cgctcttctt
     3061 tcgaccccat gcaagccaca ttgtggagga tccgggagaa gtccgccttc agtacctgcc
     3121 cttcctcgcc ttcgacgagg tccgcgtgat tcggagtgaa ttccaaggga cccactggcg
     3181 gaacagttgt ctctggaatg agcatgagct gctctgccac agcgagcgaa aacccaaaaa
     3241 ggagatcgtt tccctgcatg tcgacatcac cgatctttca tactttgaag agtagattct
     3301 tcatatcccc caagaggggc atggccattc aaagggaaaa gggtatacta cttccatgaa
     3361 gaggccgttg gttctttccc tccttctgct attcgccctc ccgatcgccg gcgggaactt
     3421 cgtcacgacg gtcgcctacg ttccgagcgc gtatctttcc attccccatt tcgaggatga
     3481 actgcccttc cgcacctcga tgagcaacac cttcatcttc gaaccgttcg gctatgaggc
     3541 ggatacctgg tcggtcaacg catcattgaa aggggcgttc gtcaccccct ccctcccttg
     3601 gggatcctat cgcgcccgag ggttcacctc cctcgggctc ggtgcccgct tctcctacag
     3661 tctcactgac cgcttcgcac tctttgg
//
LOCUS       DAOPUZ010000064         3677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_25537_length_3677_cov_2.833793, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000064 DAOPUZ010000000
VERSION     DAOPUZ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25537_length_3677_cov_2.833793"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..960
                     /locus_tag="PKN79_01875"
     CDS             79..960
                     /locus_tag="PKN79_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNZ94543.1"
                     /translation="MEATRSPMIHQPRTLNELSLLFQRIENPLLWAGGTHLMSKSDFY
                     ASPPNRQIIDLAEIEELKRISRTDRYVDIGSMVTTSSLLSAGKLVLPSILKSALGSIV
                     NHITRRQITIGGAVCEPESRMAFSTALAVLDAQAEIRRWEEGRFASAWIPVNRLYDKN
                     GSLLLRDAPFVLTKIRLGLEWGDFQRFVLIDNPIRKPDTAILVAFQATRNQTALTKVR
                     FSASFPRKAFYISPELENQLSLLNLPIHPDRVNTISYEMVHDLMNRHESITEFQAERT
                     RRVFESFLHELNIQTLS"
     gene            976..>3677
                     /gene="dnaE"
                     /locus_tag="PKN79_01880"
     CDS             976..>3677
                     /gene="dnaE"
                     /locus_tag="PKN79_01880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNZ94544.1"
                     /translation="MMTRSFSGGFVHLHNHTDFSLLDGAAKISAYMKKAQALGMDSIA
                     ITDHGNLFGALHFYYAAKKTGIKPIIGCEFYVNPSDRTERPEPGSGKKSQQYHLVLLA
                     MNQIGYQNLMILSSLSYTEGFYFRPRIDDDLLERYNEGLICLSACLGGEILQHLLNNN
                     YEEAKRRALWFNSVFDDGRYYLELQDHGLEEQKRTNPLLKRLSDETGIPLVATNDIHY
                     IEQSDANAHDLLLCVGTNAKKHDPNRMRFPNDQFYLKSREEMASLFSWVPEAIENSVR
                     IAQRCNLEIQFPGPQLPVFEVPAGYESPAEYLRSLSREGLANRFEVVTEELTERLEYE
                     LEVIISMGFEGYFLIVMDYIRWAKQQRIPVGPGRGSGAGSLVAYALDITNVDPIKYNL
                     LFERFLNPERVSMPDFDIDFCFERRGEVINYVTEKYGTDRVAQIATFGTLKAKAVVKD
                     VARVLDISFEESNRIAKMIPDDLGMTLSRAFAQSPDLVELEKKGGVYAELFDAARRLE
                     GMARHISTHAAGVVIGREDLTRYVPLYRDARTGAISTQYSMELLEPCGLVKMDFLGLK
                     TLTLIKHAQELIRVKNPAFSIEEIDEEDETTFAMLGRGESSAIFQFESPGMQNVLKEA
                     KPGTIEDLVALNALYRPGPMSFIPQYIACKHGRQPISYAVFCLKKKKKNTYGVIVYQE
                     QVMKVAQIIAGYSLGAADILRRIMSKKKVQELEAEKKKFVTGALALGRSKEHAIEIFE
                     MLEPFANYGFNKSHALAYSVIAYQTAYLKANHPAEFWAANLTNEMSNPDKFSEYLQTV
                     RAAEIEILPPSVNYSLRSFVVVGGKIVYGLAGIKHVGEGVVDAIVTERDANGPYTDLI
                     DFLTRLDSRVTNAKVMESLIKAGAFDELGTPSPWRLSLWSRGGMV"
BASE COUNT          866 a         1079 c         1002 g          730 t
ORIGIN      
        1 tgaacacctg cccgtgcatg gacgtgacgg agctgctgga gattacgatt caggcattcg
       61 agtacaggag gagaaggcgt ggaagcaact agatccccga tgattcatca gccgcgaacc
      121 ctgaatgagc tctcccttct tttccaacgg atcgagaacc cccttctttg ggcgggcggc
      181 actcacctga tgagcaagag cgacttctac gcttccccac ccaatcgcca gatcatcgat
      241 ctcgccgaga ttgaagaact gaagcgaatc agccgaaccg accgatatgt cgatatcggc
      301 tcgatggtaa ctaccagcag tctgctctcc gccggaaaac tggtccttcc ctcaattctg
      361 aaaagcgcgc tcggcagcat cgtcaatcac atcacccggc gccaaatcac gatcggcggc
      421 gcagtctgtg aaccggagag ccggatggcg ttttccaccg cacttgccgt cctcgatgcc
      481 caagcggaga tccgcaggtg ggaggagggg cggttcgcct ccgcatggat tccggtcaac
      541 cgcctctacg acaagaacgg atcgcttctg ctccgtgatg cgcccttcgt cctgacaaaa
      601 atccgtctcg ggctggagtg gggggatttc cagcgcttcg tgctgatcga caacccgatt
      661 cgaaaacccg acacggcgat tcttgtggcg tttcaagcca ccagaaacca gacggcctta
      721 acgaaagttc gtttcagcgc atcctttccc aggaaggcgt tctacatctc ccccgaattg
      781 gagaatcagc tttcactgtt gaacctgccg atccaccccg accgggtgaa caccatctca
      841 tacgagatgg ttcatgatct gatgaatcgc catgagtcga ttaccgaatt tcaagcggag
      901 cggaccagga gggtctttga gtccttcctg catgaactca acatccaaac gctctcatag
      961 gaatatattg gaggaatgat gacccggagt ttcagcggtg gcttcgtcca cctgcacaac
     1021 catacagact tttcgctgct tgacggagcg gcaaagattt ccgcctacat gaaaaaagcc
     1081 caagccctcg ggatggacag catcgccatc accgatcatg ggaacctgtt cggtgccctc
     1141 catttctact atgctgcaaa aaaaaccggg atcaagccga tcatcggctg tgaattctac
     1201 gtcaatccaa gcgaccggac cgaacgaccc gaaccgggca gcggaaagaa gagccagcag
     1261 taccacctgg ttctccttgc gatgaaccag atcggatatc agaacctgat gattctctcc
     1321 tccctttcat acactgaagg gttctacttc cgacctcgaa tcgatgatga tctcctggaa
     1381 cgatataatg aagggctcat ctgcctttcg gcgtgcctcg gcggcgagat tctccaacat
     1441 ctcttgaaca acaactatga ggaagcgaaa cgccgggcgc tctggttcaa ctccgtcttt
     1501 gacgacgggc gctactatct tgagctccag gaccacggct tggaggagca gaaacgaacc
     1561 aatccccttt tgaagcggct ctccgatgaa acgggcattc ccctcgtcgc caccaacgac
     1621 atccactata tcgagcaatc ggatgcgaac gcccatgatc tgctcctctg cgtgggaacc
     1681 aatgcgaaga agcacgatcc gaaccggatg cgctttccca acgaccagtt ctacctcaaa
     1741 agccgggagg agatggcgag tctcttttcg tgggttcccg aggcgatcga gaactccgtc
     1801 agaatcgccc aacggtgcaa tctggagatc caattccccg gtccccagct gccggtcttt
     1861 gaggtgcctg cggggtacga atcccctgcc gaatacctca ggagcctttc ccgcgaagga
     1921 ctcgccaacc gctttgaagt ggtcaccgaa gagttgaccg agcgcctcga gtacgaactg
     1981 gaggtcatca tctcgatggg cttcgaagga tatttcctca tcgtgatgga ttacatccgc
     2041 tgggcgaagc aacagaggat ccccgtcgga ccggggcgcg gttcgggggc gggttctctg
     2101 gtagcgtacg ctcttgatat caccaacgtc gatccgatca aatacaacct gctcttcgag
     2161 cgcttcctca accccgagcg ggtgagcatg ccggacttcg acatcgactt ctgcttcgaa
     2221 cgaagaggag aggtgatcaa ctacgtcacg gaaaaatatg gaaccgaccg cgtcgcccag
     2281 atcgccacct tcggaaccct caaggccaaa gcggtggtga aggatgtcgc ccgggttctc
     2341 gacatcagct tcgaggagtc caacaggatc gcgaagatga tccccgacga ccttgggatg
     2401 acgctgagca gagcgttcgc acagtcgccg gatctggtgg agctggaaaa gaaggggggc
     2461 gtgtacgccg agctctttga tgcggcccgg cgacttgaag ggatggccag gcacatctcg
     2521 acccacgccg ccggagtggt gatcggaagg gaggatctca cccggtacgt tcccctgtac
     2581 cgcgatgccc ggaccggcgc gatctccacg caatactcga tggagctgct tgaaccgtgc
     2641 ggtctggtga agatggactt tttggggctg aagaccctca cattgatcaa gcatgcccag
     2701 gaactgatcc gggtcaaaaa ccccgccttc tcgatcgaag agatcgatga agaggatgag
     2761 acgactttcg cgatgctcgg ccgcggagag tccagcgcta tcttccagtt tgaaagcccc
     2821 ggaatgcaga acgtcctcaa agaggcgaag cccggcacga tcgaagatct cgtcgccctc
     2881 aacgccctct atcgccccgg accgatgagt ttcattcccc agtacatcgc ctgcaagcac
     2941 ggccgtcagc cgatctcgta tgccgtcttc tgcttgaaaa aaaaaaagaa aaacacctac
     3001 ggggtgatcg tctatcagga gcaggtgatg aaagtcgccc agatcatcgc aggctactcc
     3061 ctgggagccg cggatatcct gaggcggatc atgagtaaaa agaaagtcca ggaactggaa
     3121 gcggaaaaaa agaagttcgt cacaggagcg ctcgccttgg gcaggagcaa ggagcatgcg
     3181 atcgaaatct tcgagatgct cgagccgttc gccaactatg gcttcaacaa gagccatgcc
     3241 ctcgcctact ccgtgatcgc ctatcagacc gcatacctga aggcgaacca tcccgccgag
     3301 ttctgggcgg cgaacctcac caacgagatg agcaaccccg acaaattcag cgagtacctc
     3361 caaacggtcc gggcggcgga gatcgagatt ctccctccca gcgtgaacta ttcgcttcgc
     3421 tcgttcgtcg tcgtaggcgg aaagattgtc tatggccttg cgggcatcaa gcacgtcggc
     3481 gagggggttg tggacgcgat cgtcaccgaa cgcgacgcca acggtccgta cacagacttg
     3541 attgatttcc tgacccgcct cgactccaga gtgacaaacg ccaaggtgat ggagtccctg
     3601 atcaaagcgg gggcgtttga tgagctgggg actccatcac cttggcgttt gtcactctgg
     3661 agtcgaggcg ggatggt
//
LOCUS       DAOPUZ010000065         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_25583_length_3672_cov_2.681504, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000065 DAOPUZ010000000
VERSION     DAOPUZ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25583_length_3672_cov_2.681504"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /locus_tag="PKN79_01885"
     CDS             <1..1654
                     /locus_tag="PKN79_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNZ94545.1"
                     /translation="HLERALRLFSIPYTVQIARSLFLEAIINDFHAMLQLLIHPSDRL
                     SYAVVLRSPFCFIDDSALVELLQSEELFTVVDTLSEVDKKKMVRMKHFFEDLKEAATV
                     QRLERLIFMLWHESGYAHHYIQNPDYHSDIEHYELLYRLAQTFQEKGRTLSEFLDYLR
                     NRLGRNERPDEMDLIKETKEGVQIMSVHKAKGLEFPIVIVANAGVTILGGGNSPPSNY
                     PYPLPDFFDQMILMGEKGEEKQIGNIDELFNDKTSGSQELAELKRLLYVALTRAETHL
                     IISGYFHRNNRNFKGKLSSFLQHLCKALGIENPEDPSLEHGKVRLELIEDVPSDVFYR
                     DLEADVPIEELKPWYESVDAPYQAEALRYAVTALAPEVERTFVKELPAIASDVFLGEK
                     ECSALFGTFVHRLIEAHLKKEDLSDPLFYMEDSFKELLTTRETEAVLSDALLLSDNFI
                     HSRLCVDEVLDHPFKSEMSFFSRLQHEGRTVVAEGVIDLLVDRGDEILVIDFKSDRFV
                     DEEVHRFQIETYIDAARRLYKKKTSGLIVYLRDIARSIRYEG"
     gene            complement(1707..3532)
                     /locus_tag="PKN79_01890"
                     /pseudo
     CDS             complement(1707..3532)
                     /locus_tag="PKN79_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607906.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
BASE COUNT          758 a         1056 c         1012 g          846 t
ORIGIN      
        1 tcaccttgag cgggctctga ggctgttttc gattccctat acggtgcaga tagcccgttc
       61 cctctttctg gaagcgatca tcaacgactt ccacgccatg ctgcagctcc tgattcaccc
      121 gtcggaccgg ctctcctacg cggtggtgct tcgctcgccg ttctgtttca tcgatgacag
      181 cgccttggtc gagctccttc aaagcgagga actcttcacc gtcgtcgata ccctttccga
      241 ggtcgataaa aagaaaatgg tacgcatgaa gcacttcttc gaggatctga aagaagctgc
      301 caccgtccag cgcctggagc gcctcatatt catgctgtgg catgaaagcg gctatgccca
      361 ccactacatc cagaaccccg attaccactc ggacatcgaa cactatgagc tgctctatcg
      421 actcgcccag acgtttcagg agaagggcag gacgctcagc gagttcctcg attatcttcg
      481 gaatcggctc ggacggaatg aacgcccaga tgagatggat ttgattaagg aaacgaaaga
      541 gggtgtccag atcatgtcgg tgcacaaggc aaagggcctt gagttcccga tcgttatcgt
      601 cgccaatgcc ggagttacaa tcctcggtgg cgggaacagc cccccatcca attatccgta
      661 cccccttccg gacttttttg atcaaatgat tctgatgggt gaaaaaggag aggagaagca
      721 aatcgggaac atcgacgagc tcttcaatga caagacgtcc ggcagtcagg agctcgccga
      781 gctgaaacgt ctcctctatg tcgctctcac ccgggcggaa acccacctga tcatcagcgg
      841 atacttccat cgcaacaatc gaaacttcaa gggaaagttg tcatcgtttc tccagcatct
      901 ttgcaaagct cttggaattg aaaaccctga agatccgagt ctggaacacg ggaaggttcg
      961 cttggagctg atcgaagatg ttccttccga tgtgttctat cgagatcttg aagcagacgt
     1021 gccgatcgag gagctgaagc cctggtatga gagcgttgac gctccctatc aggcggaggc
     1081 gctccggtat gcggtgacgg ctctcgctcc cgaggttgaa cgtacgttcg tcaaggagct
     1141 gccggcgatc gcaagcgacg tcttccttgg cgaaaaggaa tgctccgctt tgtttggaac
     1201 gttcgttcat cgtctgatcg aagcccatct taaaaaggaa gacctttccg atccgctctt
     1261 ctatatggag gattccttca aagagctgct gaccacgaga gagacggagg cggttctctc
     1321 cgatgcgctt ctcctcagtg acaacttcat ccacagccgg ctgtgtgtgg atgaggttct
     1381 cgatcatccc ttcaagtcgg agatgagctt cttcagccgc ctgcagcacg aaggccggac
     1441 cgtcgtagcg gagggggtga tcgacctctt ggtcgaccgg ggtgatgaaa tactggtcat
     1501 cgacttcaag agcgatcgct tcgtcgatga agaggttcac cgcttccaga tcgagaccta
     1561 tatcgacgca gcacgtcggc tctacaagaa gaagaccagc ggcctcatcg tctatctccg
     1621 ggacatcgcc cggagcatcc gctacgaggg ataataaaga agaggaggcc ggaattccag
     1681 cctcctcaac cgaatgaatt gcgtttttag tatttgccgt ataacggacc gaatgtcgcg
     1741 caggcacgga agtccgagcc ttccttgtcc atcccaagca tcgaccaggc actggggcgg
     1801 aagatatcct tttccgccac gttgtgcatg ttgaccggaa tccggagcat cgaagcgagg
     1861 gtgatcaagt cggcgccgac gtggccatag gcgatcgccc cgtggtttgc tccccagttg
     1921 gccatcacgg agtagacatc cttgaaggga ccttcatgct ccacaaggcg gggaacgaac
     1981 caggtggtgg gccaggtctg gtcggttcgc ttgttgatga tctcaaagac ttcctcagga
     2041 acatcggctg tccatccctc ggcgatctga agaaccgggc cgacgccctt gaccatgttc
     2101 agccgaagca tcgtaacggg cattccgcct tcacttaaga aggtggagga gaaccctccg
     2161 ccccggaaat atccgaggtt tccgacgctg aagcgggtgt tgtccaacgt cttctggaca
     2221 tcctcggcgg tgatctccca atagggcttc atcgccggct cacctttttc gttggtcatc
     2281 tgtccggcgg cgtcgagggt cgtcgctccg ctgttgatca ggtggatgaa tccgtctttc
     2341 gccaacccgg tgggcttcca gccggttacc cgttcgacgc tcttgggact ccagtatgtc
     2401 cgcacgtcgc tgaagatcac cgccctgttg gtcaagagct tcgcaaagag catgctgatc
     2461 ccgttgaggt ggtcgttctc ggtggccatg atgtacggct cccgaatgcc gttccagtcg
     2521 aagctggaag tgagcatcgt ctccatgaag tcgccgttcg gccagtggtc ggtccattgg
     2581 cgctggccct ggaagcccgc ggcgatcgca ttgcggcata ggactcctct ttgaatccga
     2641 gggtgttgag cttggggttt ccgatcatca ggtcccggcc gatcatcacc atcttggtga
     2701 cgtactccca atccttgtcc agctcttcct ggctcttgcg gagggcggga tcgttgacgg
     2761 tgtcctccgc ctggatgaca tgcttgcgga cccattcaat cgctttcttg aactcgacgg
     2821 gatcatagat ctcttcagct atccgccgct cgatttcact catgtccacg acttcggttc
     2881 gcatgccaag gtacgcctga aggaaatcct ggtcgacaat cgagccggcg attcccatcg
     2941 acatccctcc gatcgagagg tagctctttc ccctcatctc ggcgaccgcc agaccggctt
     3001 tggcgaagcg gaggatcttc tcctggacat cctcgggaat cgaggtgtcc gtcgagtcct
     3061 gaacatcccg gccgtagatg ccgaaggcgg ggaggccttt ctgggtgtag gcggcaagaa
     3121 ccgccgcaag gtacaccgcg ccggggcgct cggtgccgtt gaacccccag accgccttga
     3181 ccgtcagcgg gtccatgtcg aaggtctccg ttccgtagca ccaacacggg gtgacggtca
     3241 gggttacagc gaccccttcc tttttgaact tctcggcgca atcggcgctc tggcgcactc
     3301 cgccgatcgt ggagtcggcg atgacgcact ccacctgctc accgttcgtg tggcgaagat
     3361 tgtcgctgat gagcttcgct gcggccttgg ccatattcat cgtctgctct tccaacgatt
     3421 cgcggacacc ccgttggcgt ccgtcgatga ccggacggat tccgatcttc ggaagctctg
     3481 aagcgtaacg cctctccggt cctacctgtt taatatcaag aatgtttgcc atctcgttct
     3541 ccttggtgta tgatggtttt gtaatcgttt acatactctc agtatcgcac agcatccaag
     3601 cgattgcaac gaaaaaaagt gagcttcaca ccatcacgtc cggtatggag cattaggtgg
     3661 tgagagtgaa gt
//
LOCUS       DAOPUZ010000066         3651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_25782_length_3651_cov_3.298109, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000066 DAOPUZ010000000
VERSION     DAOPUZ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25782_length_3651_cov_3.298109"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /gene="mutS"
                     /locus_tag="PKN79_01895"
     CDS             complement(<1..1794)
                     /gene="mutS"
                     /locus_tag="PKN79_01895"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNZ94546.1"
                     /translation="MSKTQRTDTPMMAQYRSIKEQHPDSVLFFRLGDFYEMFEDDAIE
                     VSRLLNITLTARGGVPMAGVPYHAAKNYIKRLLDAGKKVAICEQTLIGEKLAQREVVQ
                     VVTPATVIEDDFLVASDANFLMAVTIQKGQIVTGYADLGSGRFFVRQVEHDPGYIHLL
                     ALLEEVRPRELLVNEDEYFLLGEFKEAIDQTSAMVTKLPPYQFSAKKAYESLLEHFEV
                     HSLKAFGIDDHAPYLSSAGALLSYLQQTARSSLKQIETLTLVHSAEFLQIDEDSRKSL
                     ELFANLSDGSPRYTLFDSIDSTVTSMGTRKLKQWVASPLARKETIFSRHRWVDLLYHD
                     REELERVRHELKQVLDLERLTSRVVMGRHLPKDITGIAQALRGFFLLFDERYRDLLVT
                     TQQEHEKAFALATHIDRALNPTHQGPFEEGRVILDGYDSELDELRKIKGGGKELLAEY
                     LEEIKVQTGIPTVKLGNNRILGHYLEVTKIHSDKMPATFYRKQTLVNAERFTSDELIA
                     LERKIQHAVLDAEQYERALYEHLVEETLAAQKTLLAIAAFLSDLDCYQSLAYRAHISS
                     FVKPEFVDEDCLIVEGGRHPVVEANLPPGEFV"
     gene            complement(1796..2920)
                     /locus_tag="PKN79_01900"
                     /pseudo
     CDS             complement(1796..2920)
                     /locus_tag="PKN79_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
     gene            complement(2939..>3651)
                     /locus_tag="PKN79_01905"
     CDS             complement(2939..>3651)
                     /locus_tag="PKN79_01905"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNZ94547.1"
                     /translation="KKAIIQYYQNRGYIDVEVGSAYVEDISSDDDKYTRLRLVYPIAE
                     GSQWFFGGIEVSGNEVFSDEQFQNLISIKTGSVLDLSRIQAQIAALTDLYWNNGYIFN
                     LISSDEVRNEENKTITYRLHVQENRQAIIEDVRIEGITRSKPYVFERELAFKKGDIFS
                     KELLIKSAQNIYNTLIVTDVQFDILNGTEEGTVIPVFTVTEGNQMDLQFGATFGGNVD
                     GFPVSGFLQWSNKNLGGT"
BASE COUNT          792 a          959 c         1010 g          890 t
ORIGIN      
        1 gacaaactcg ccggggggaa ggttggcttc gaccacggga tggcgtcctc cttcgacgat
       61 gaggcaatcc tcgtcaacaa actccggttt cacgaatgag gagatatggg cccgataggc
      121 caggctttga tagcagtcaa gatcgcttaa aaacgcggcg attgccaaga gggtcttttg
      181 ggcggcgagg gtttcttcaa ccagatgctc atacagggct cgctcatact gctccgcatc
      241 cagcaccgcg tgctggatct ttcgctccaa ggcgatcagt tcatcgctgg tgaagcgctc
      301 ggcgttcacc agcgtctgtt tgcgataaaa cgttgcgggc atcttgtcac tatggatttt
      361 ggtcacttca agatagtggc cgagaattcg gttgttgccc agcttgaccg tcggaatgcc
      421 ggtttggacc ttgatctctt caagatactc cgccagcagc tcttttcctc cgcctttgat
      481 ctttctgagc tcatccagct cggaatcgta tccgtcaaga atcaccctcc cctcctcaaa
      541 gggaccttgg tgggtcggat tcagcgcccg atcgatatgg gtcgccaagg caaaggcctt
      601 ttcatgttcc tgttgcgtgg tgacaagaag atcccggtaa cgttcatcga agagaaggaa
      661 aaagcccctc agtgcctgag cgattccggt tatgtccttg ggcaggtgac gacccatcac
      721 caccctgctg gtcaaccgtt ccaggtcgag gacctgtttg agctcgtgtc gaacccgttc
      781 cagctcctcg cggtcgtgat atagaagatc aacccagcgg tggcgggaaa aaatcgtctc
      841 ttttctcgcc aaaggggaag cgacccattg tttgagtttt cgagttccca tcgaggtgac
      901 cgtcgagtcg atcgaatcaa agagcgtgta tcgcggcgat ccgtcactga ggttggcaaa
      961 aagctcgagc gattttctgg aatcctcgtc gatctgcaag aattcggcgg aatggaccaa
     1021 ggtgagagtt tcaatctgtt tgagggagga gcgggcggtc tgctggaggt aggataagag
     1081 tgcaccggcg ctcgaaagat agggagcatg gtcatcgatg ccgaacgctt tgaggctatg
     1141 gacctcaaag tgttccagca aactctcata cgccttcttt gccgagaatt gatagggagg
     1201 gagctttgta accatcgccg atgtttgatc gatcgcctcc ttgaactccc ccagcagaaa
     1261 gtactcatct tcattgacca gcagttcacg gggacgaacc tcttctagaa gggcaagcag
     1321 gtggatgtac ccgggatcgt gctccacttg gcggacgaag aaccttcccg aaccaagatc
     1381 ggcgtatccg gtgacgatct gacccttctg gatcgttacc gccatcagga agttcgcatc
     1441 gctcgctaca agaaaatcat cctcgatgac cgtcgccggg gttacaacct gaacgacttc
     1501 ccgttgggca agtttttctc cgataagggt ttgctcgcag atggcgactt ttttcccggc
     1561 atccaggagc cgtttgatgt agtttttcgc cgcatggtag ggaaccccgg ccatggggac
     1621 tcctccccgg gcggtcaagg tgatgttcaa gaggcgcgag acttcgatcg catcgtcttc
     1681 gaacatctca taaaaatcac cgaggcgaaa aaagagaacc gagtcgggat gctgctcttt
     1741 aatcgaacga tattgagcca tcatcggagt gtcggttcgt tgtgttttac tcatatcaat
     1801 acaacgtcgt cgtgatcgca agaatcagtt tcaagccgct tgtgtcgttc tgtcccctga
     1861 agaggaagtg gttcgtatcc catgcgaagg tgttgtcgat ataggtcgca ttcttcacca
     1921 ggtaaagccc gagcgggaac ccgggaacct tgagcttgat tccgccgccg gcggagaagt
     1981 accaggcaag ctgatccatc ttcgtttcct tgaggctttg gcttaccccg cttgccgaga
     2041 tgtagagttc ggcggaaagc acgttgtgca cgagcggcca ggagacggag agctgggtat
     2101 cccaaaggaa tgcctggtca aagacgaccg ggaagcctcg ggcagtattc atcccgtcga
     2161 ggtagagcat ttcataccgg gtcgctccca tcttcgcgtc ataccaggcc cacccctgct
     2221 cgccgttctg ggttacggga ttatggaact gcggcagcat cgccccgacc gtggtcgtca
     2281 tgccaagcac cacgttcgcc ggtttctcct caagctggaa gctgaagagg gtgagatacc
     2341 cgctgaggct ggtcgtggtt cgcatatagt tggaaagacc gaagaggatc cctccggcat
     2401 aggtgaaggt ctcggagata taatacccgc ggctcgtgtt ttcgatccgg tcccgtccgt
     2461 cccaagcgaa ggtgagcgag aggcggtttg agaattgcca cttttctcca tactgcttga
     2521 tcagccactc atagggatcg tagatggcct tatcgtactc cgccttgttc agtccgatcg
     2581 acacccccac cccgatcgaa agcgcaccgg gacggaacat gaacgtatat cccgtgttat
     2641 atccgaggga gaagcggaat tgcacatatt ccatcagctt gtcagcggtg gggtatgcct
     2701 ggcccgccgc ctgatatgcc tgatagcttt catatccgtt cggataggtg ttcgcgctgg
     2761 tcgaggtggt gaagtaacca ctgccctgac ctcgggtgag tgtattttct ttcacacttc
     2821 gttcaaagga gaaggtgaac ccgtttgacc atcggtagga tctgaaccaa tcatcctgga
     2881 aggcgatcat caccgtttgg gtatcggggc tgaggttggt tgaaacggag aggacccgtc
     2941 aggtgccacc caagttcttg tttgaccact gcaaaaaccc gctgacgggg aatccgtcga
     3001 cgttgccgcc gaatgtcgct ccgaactgaa gatccatctg gttcccttcc gtcaccgtga
     3061 agacgggaat gaccgttcct tcctccgttc cattgaggat gtcgaattgg acatcggtga
     3121 cgatcagggt gttgtaaatg ttttgagcgg atttgatgag cagctctttg gagaagatat
     3181 cccctttctt gaatgcaagc tcccgttcga agacgtatgg tttggagcgg gtgattccct
     3241 caatccgcac atcttcgatg attgcctgcc ggttctcctg aacatggagc cgatacgtga
     3301 tcgtcttgtt ttcctcattc cgaacctcgt cgctgctgat caggttgaag atgtatccgt
     3361 tgttccaata cagatcggtg agcgccgcga tttgagcctg aatccgggag agatccagaa
     3421 ccgagccggt ttttatcgag atgaggttct gaaactgttc atcgctgaac acctcatttc
     3481 cgctgacctc gattccgccg aagaaccact gactgccttc ggcaatcggg tagaccagcc
     3541 tgagacgggt atatttatca tcgtcggaag agatgtcttc aacataggcg gagcccactt
     3601 cgacatcgat atacccgcgg ttctgatagt attggatgat cgcttttttg t
//
LOCUS       DAOPUZ010000067         3649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_25807_length_3649_cov_2.926822, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000067 DAOPUZ010000000
VERSION     DAOPUZ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25807_length_3649_cov_2.926822"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PKN79_01910"
     CDS             complement(<1..502)
                     /locus_tag="PKN79_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNZ94548.1"
                     /translation="MEKRITFDSEELVRRVLGINDRNIPYMETLLGCELFVRSNDVIC
                     LSDDPLIQNRFLLLFGRLSRLAEHQLFLNETDIFMEFQSIKNDVSQDRDVVNGDRETR
                     KTIKIHNRRAYAKGANQERYVELMEENKIVFAIGPAGTGKTFLAVAYALSQLFSGRKR
                     KIILTRP"
     gene            complement(518..3394)
                     /locus_tag="PKN79_01915"
     CDS             complement(518..3394)
                     /locus_tag="PKN79_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94549.1"
                     /translation="MKKARVASSFCFLVFLLALCVNGSLFGRESGQEYILNIINADGF
                     ESLEGGRYLLKGNVHLMIEEEGSEEKIRLYSDRLLVDVSDKSLTAMGSVRTEGAKEIS
                     GEIITLMYERGDIFASCADLVTISEELGDQPVTLHTLGTRITIQSASSMITYTDARIG
                     TRAIDPLSSIRAKRISVLNDGDIIAHGATLSIGRVPLFWTPFLFIPGSRMIANPAIGF
                     ISNRGMFVNTTWEIFGRYPHFRKDEQRSLATLLSAKEETGAIRAWPIYTLEKAPSSFE
                     QWARKSSSYLAIFGDAYEDTGVSLGYDSRIKLLKNTLTINSLSTVALDPSGIDQRVAW
                     SDVNKVRMWSMNTLFYRVGSSSLTATLPYYSDPSVERLYSSRLHNLRLDALFGSVQEF
                     PPLGSERSSYTMGLTGNLTIPTTIFKEYLSSLTIDNIALQGTYRWQKKDGIHAYHLTE
                     VILPRFIIRASGTLLNLAGQTTVKSPPVSNTPDVNPLLPNLYRPQRQQPASLFSRQAE
                     RSLKLSYTISEEFARTYNKTAGDDPNVYSMTRGTMILSAVPDSRFFTSSFELLPQLSV
                     SDDPQKTTNHTQVFQLHANTTLHLPFIGVSYTLRQRLYRQEETQSGDKPKNVVIDRFR
                     FDEKSVSTHQLRFDRTYRLWAGTINPSITAVLWPLTRTLAPKVIVRYQGASLTTSLRF
                     KDASGTGFLEKELFSLGVGYSDSLLVTSTTFSYNYLKPLEPSRLDHQLTLSLFDKELV
                     LSERLEYFPMKNGVEHVLSRGVLGLKTSFLTLNYHAGGTLGNIQSERLTAMVSLAERQ
                     WRFYKKRIALTLGVNAAFDYDVDDPFSSVFTFSLKSRFQIAEFLDLSIALASSNTGFH
                     HYQDDAGSFAWLLLWKDLLRSFDLFGGGIHHTQFNLSSVDIQLIHYLEDWRLNCKYSG
                     SVVLSNNQYRWVPTFSIYLAWNTIPDLDIEEKWSRTGGVWDRSL"
     gene            complement(3381..>3649)
                     /locus_tag="PKN79_01920"
     CDS             complement(3381..>3649)
                     /locus_tag="PKN79_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94550.1"
                     /translation="GGRCFPGRPADSEPQAASSHRLVDQGNTVLLIEHNLDVIAQADH
                     LIDLGPEGGDGGGMVVASGTPEQLALCKDSYTGYYLAQMFHEKG"
BASE COUNT          847 a          910 c         1020 g          872 t
ORIGIN      
        1 cgggccgggt gaggatgatc ttgcgcttcc ggccgctgaa aagctggctg agggcgtagg
       61 cgaccgcaag gaaggtcttc cccgtccctg ccggcccgat agcgaagacg atcttgttct
      121 cctccatcag ctcgacataa cgctcctggt ttgctccttt ggcgtaggcg cggcgattgt
      181 gaatcttgat tgtttttcgg gtctcccgat ccccgttgac cacatccctg tcctggctca
      241 catcattctt gattgattgg aactccatga agatatccgt ttcattcagg aagagctgat
      301 gttcggcgag tcggctgagc cggccgaaga gcaggaggaa gcggttttga atgagcggat
      361 cgtcactgag acagatgaca tcgttcgagc ggacgaacaa ctcgcacccc aataacgttt
      421 ccatgtatgg gatatttcga tcgttgatgc cgaggactcg gcgaactaat tcttcactgt
      481 caaaggtgat tcgtttttcc atacccatcc tatcgcatta cagggaacga tcccaaacac
      541 caccggttcg cgaccatttc tcttcaatat caagatcggg aatcgtattc catgccaggt
      601 agatgctgaa ggtcggcacc caccgatatt gattgttcga tagtacaaca cttccactgt
      661 atttacaatt cagtctccaa tcctccagat agtggatcag ctggatatcc accgaggaga
      721 ggttgaattg ggtatgatgg attccccctc cgaagagatc gaaactgcgc agaagatcct
      781 tccaaagaag aagccaggca aaggagccgg catcgtcctg atagtgatgg aagccggtgt
      841 tggaggaggc aagagcgatc gacagatcga ggaattcggc gatttggaag cgactcttca
      901 aggagaacgt gaagaccgag gagaagggat catcaacatc gtaatcaaat gctgcgttca
      961 ctccaagggt cagagcgatc cgtttcttat agaagcgcca ctgcctttca gcgagactca
     1021 ccatagcggt gagccgctcg ctctgtatgt tgccaagggt tccacccgca tggtagttca
     1081 atgtgagaaa ggaggttttc agccccagca ccccacggga aagaacgtgt tcgactccgt
     1141 ttttcattgg gaagtactca agtcgttcac tgaggaccag ttccttgtca aagagcgaaa
     1201 gggtgagctg atgatccaga cgggagggtt ccaacggttt gaggtagtta taggagaagg
     1261 tggtgctggt cacgaggagg gaatcgctat atccgactcc aaggctgaag agctccttct
     1321 ccagaaatcc ggttccgctt gcatctttga atcgcagcga tgtcgtcaaa gatgctcctt
     1381 gatagcgcac aatcactttg ggagcaaggg tccgggtgag cggccacaga actgcggtga
     1441 tcgaaggatt gatcgtaccc gcccataagc gataggtccg atcgaaccgc agttgatgag
     1501 tgctcaccga tttctcatca aacctgaagc gatcgatgac cacgttcttc ggtttgtcgc
     1561 cgctttgagt ctcttcctgg cgatacagcc gttggcgaag agtataggat acaccgatga
     1621 acggcaggtg aagcgttgtg tttgcatgca gttggaagac ttgggtgtgg tttgtggttt
     1681 tttgcgggtc atcgctgact gagagctgtg gcagcagctc aaaggaggag gtgaagaagc
     1741 ggctgtcggg aaccgctgaa agaatcatgg ttcctctcgt catcgaatac acgttagggt
     1801 catcccccgc cgtcttgttg tacgttcgag cgaactcctc gctgatcgta taggagagct
     1861 tcaatgagcg ctcggcttga cgtgaaaaga gcgaggcggg ttgctgccgt tgggggcgat
     1921 agagattcgg cagcaaagga ttgacatccg gagtatttga aaccggaggg ctttttaccg
     1981 tggtctgccc ggcaaggttc aaaagtgttc cgcttgctcg gatgatgaac cgcggcagga
     2041 tcacttcagt gagatgatat gcatggattc catctttttt ttgccaacga tatgttccct
     2101 gaagagcgat attgtcgatc gtgagcgaag aaagatactc tttgaagatc gttgtgggga
     2161 tcgtgaggtt tccggtcaaa cccatcgtgt aggaggagcg ctccgaaccg agggggggaa
     2221 attcctgtac cgaaccgaag agcgcatcca accgaagatt gtgcaagcgg cttgaataga
     2281 gccgctcgac gctcggatcg ctgtagtatg gcagggttgc cgtaagagag gaggacccca
     2341 ccctgtagaa gagcgtgttc atgctccaca tgcgcacttt gttgacatcc gaccaggcaa
     2401 cgcgttgatc gatcccggat ggatcaaggg cgacggtgga aagcgaattg atcgtgagag
     2461 tgttcttcag cagcttgatc cgggaatcat atccgaggct caccccggta tcttcgtatg
     2521 catcaccgaa aatcgccaga tatgaggagg atttccttgc ccattgctcg aatgaagatg
     2581 gagctttctc cagtgtgtat atcggccatg cacggatggc tcctgtttcc tctttcgctg
     2641 acaacagggt tgccaacgac cgttgttcat cctttcggaa atggggatat cttccgaaga
     2701 tttcccatgt ggtatttacg aacatccctc gattgctgat gaaccctatc gccggattgg
     2761 cgatcatccg tgaaccggga atgaagagaa atggtgtcca aaaaagcgga acccggccga
     2821 ttgaaagggt cgccccatgg gcgataatat ccccgtcgtt cagtacgctg atccgctttg
     2881 cccgaatcga tgaaagcgga tcgatcgctc gggtgccgat ccgggcatcg gtgtaggtga
     2941 tcatcgaact cgcgctttga atcgtgatcc tcgttccaag ggtgtggagg gtgacaggct
     3001 gatcaccgag ctcttcgctg attgtaacca gatcggcgca ggaggcgaag atatctcccc
     3061 gttcgtacat cagggtgatg atctctccac tgatttcctt cgcgccctcg gtacggaccg
     3121 aacccatcgc tgtcagactt ttatcggata catcaacaag gagtcggtcg ctgtagagac
     3181 ggattttctc ctcgctgccc tcttcttcga tcatcagatg aacgtttcct ttgaggagat
     3241 atcgtccccc ttccaaactc tcaaatccgt cggcgttgat gatgttgagg atgtattcct
     3301 gcccactctc acgaccgaac aatgaaccgt ttacgcacag tgcgagcagg aacacgagga
     3361 aacagaaaga tgatgcgacc ctagcctttt tcatggaaca tctgagcaag ataatacccc
     3421 gtgtaagagt ccttgcatag ggcgagctgc tccggcgttc ccgaggctac gaccattcct
     3481 cctccatccc cgccttcggg gccaagatcg atcagatgat ccgcttgagc gatcacatcc
     3541 aggttgtgtt cgatcaggag gacggtgttt ccctggtcga ccagccgatg tgaagaagct
     3601 gcttgaggtt ctgaatcggc tggtcgacca gggaaacacc gtcctcctg
//
LOCUS       DAOPUZ010000068         3640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_25896_length_3640_cov_3.776290, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000068 DAOPUZ010000000
VERSION     DAOPUZ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25896_length_3640_cov_3.776290"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKN79_01925"
     CDS             <1..246
                     /locus_tag="PKN79_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNZ94551.1"
                     /translation="PELEVLGILFTMYSKRTRLSRDVVDDIVSYFDDLVFDTMIPRNI
                     RLAEAPSHGLPINQYDGSCNGAKAYRALAKEVIKRGK"
     gene            236..1165
                     /locus_tag="PKN79_01930"
     CDS             236..1165
                     /locus_tag="PKN79_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNZ94552.1"
                     /translation="MESKRKIGLGKGIGSLMQDFSYDSVLDNALSSAKKEEKGDRVLE
                     VELDAVRPNPNQPRKEFDNATLEELAQSIKREGVLQPILVEEIGPNRYSIVAGERRYR
                     ASKMADLKTIPVLVKDVTQMQRLEISLIENIQRESLNPIEEAQAYAYLIQEAGITQEE
                     LARRLGKNRSTISNSIRLLQLNPVMQDELLKGKFTAGHARAILSVVNPADREILYKTL
                     LERDLSVRATEEMATQFNQGRRVAHQKKKRSKKEAEKSLEILAAEESFLMAFGTQVEI
                     KGTIKKGRIEIPFSSTEELERIYQILVPNEHLF"
     gene            1177..2118
                     /locus_tag="PKN79_01935"
     CDS             1177..2118
                     /locus_tag="PKN79_01935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ94553.1"
                     /translation="MDTKTLKDGLYAVMHTSKGDILLALEYKKTPMTVANFVGLAEGS
                     LNKDKPFYDGLKFHRVIANFMIQGGCPLGTGTGGPGYKFGDEFDPSLKHDRAGILSMA
                     NSGPGTNGSQFFITHVETPWLDNRHSVFGHVVEGQKVVDSIAQGDTIKKVEILRVGSD
                     AEAFKVTKQIFTSYIVESENKRKKALEEARANVEAELKNRYPEAIRTKTGLRYVVKKK
                     GDGERFPKMGTRVKVHYQGSLLDGRIFDSSIQRGEPTEFRIGEVIEGWNEALVTMSAG
                     EERTLIIPPELGYGEMGYPGIIPPNSYLIFEVHLLSF"
     gene            2131..3486
                     /gene="radA"
                     /locus_tag="PKN79_01940"
     CDS             2131..3486
                     /gene="radA"
                     /locus_tag="PKN79_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNZ94554.1"
                     /translation="MAKRSGDLQFVCSTCGRTEAKWLGRCPDCGSWNSFEEELIVAPS
                     KGAKPLSTDEEPVRLEDVVVDPQFRYASGISELDRVLGGGVMKGSSVLIGGEPGIGKS
                     TLMLQLLQSTRNTSKVLYVSGEESPSQVKMRAQRLGLHLSKINIFCDTRLEVLEKVLP
                     SSKASLVVIDSLQTLSSDTLPSPAGSVNQIRHCSNALVSLAKRLGISLFFVAHVTKEG
                     TIAGPKIIDHLVDTVLYFDQLPSGIRLIRAAKNRFGSVDEIGLFSMDEKGLHPVANPA
                     SFFLSDRAEGPLPPGVAYTAVAEGTRSFLVEVQALTVPAKSGFSRIFSDRIDSARVTR
                     IAAVLERHAGLRLSDQDIYVNVGGGMKVGEVAVELALALALYSALTRTSLPKDLVSFG
                     ELSLSGEIRPVGFIQRREKTAKELGFTRSLVSKASKQSSLTQYGARTIRDALDAVREM
                     H"
BASE COUNT          939 a          879 c         1023 g          799 t
ORIGIN      
        1 ccggagctgg aagtactggg aattctcttt acgatgtaca gcaagcgaac ccgtctctca
       61 cgggatgtcg tcgacgatat cgtctcctat tttgatgatc tggtgtttga tacgatgatt
      121 cctcggaata tccgtctggc cgaagccccc agccatggtt taccgattaa tcagtatgac
      181 ggctcctgca acggagcgaa ggcgtatcga gctttggcga aggaggtcat taaacgtgga
      241 aagtaaacga aagattggat taggcaaagg catcggttca ttgatgcagg atttctccta
      301 tgattcagtg ctggacaacg ccttgagctc agccaaaaag gaagagaagg gagaccgggt
      361 tttagaagtg gagcttgatg cggttcggcc caacccaaac cagcctcgga aagagtttga
      421 caacgcaacg ttggaggaac ttgcccagtc gataaaacgt gaaggggtct tgcagccgat
      481 cctggtcgaa gagatcggtc cgaaccggta ttcgattgta gccggggaac ggcgataccg
      541 ggcatccaag atggcggatt tgaaaaccat tcccgtactg gtgaaagacg taacgcagat
      601 gcagcgcctt gaaatctccc tgatcgagaa tatccaacgg gaatccctca atccgatcga
      661 ggaagcgcag gcgtacgcct acctgatcca ggaagccgga atcacccagg aagagcttgc
      721 ccgacgactg gggaagaatc gatcgacgat tagcaacagc atccgactgc ttcagctcaa
      781 ccccgtgatg caggatgagc ttcttaaggg caagttcacc gccggacatg ccagggcgat
      841 tctctcggtt gtaaacccgg ccgatcggga aattctgtat aaaacattac ttgaaagaga
      901 tttatctgtt cgggcaaccg aggagatggc cactcagttc aaccagggta ggcgagtggc
      961 gcatcaaaag aaaaagcgat ccaagaaaga agcggaaaag agtctggaga ttctagccgc
     1021 cgaggaaagc tttctgatgg cgttcggcac ccaagtcgaa atcaagggta cgatcaagaa
     1081 aggcaggatt gaaatcccct tttccagcac ggaagaactg gaacgaatat atcagatcct
     1141 cgttcccaac gagcatttat tttgaggaag gaattcatgg atacgaaaac tctgaaggac
     1201 ggcctctatg ccgttatgca tacgagcaag ggggatatac tgctcgccct tgaatataag
     1261 aaaacaccga tgaccgttgc aaacttcgtc ggccttgccg agggaagttt gaataaggat
     1321 aaacccttct atgatgggtt gaagtttcac cgggtgatcg cgaacttcat gattcagggc
     1381 ggatgtcctc tggggacggg aaccggtgga ccggggtaca agttcggtga tgaattcgat
     1441 ccttccctga aacacgatcg agcgggaatt ctttcgatgg cgaacagcgg ccccggaacg
     1501 aatgggagcc aattcttcat cactcatgta gaaacccctt ggcttgacaa cagacattcg
     1561 gtcttcgggc acgtcgttga aggtcagaag gtcgtcgatt caattgctca aggcgatacg
     1621 atcaaaaagg ttgaaattct tcgagtcggc agtgatgccg aagccttcaa ggttacgaag
     1681 caaatattta catcatatat agttgaatcg gaaaacaagc gaaaaaaagc tctcgaagaa
     1741 gcacgggcga acgtggaggc ggaactgaag aatcgatatc ctgaagcaat tcgtacaaag
     1801 accggcctta ggtatgtagt aaaaaagaag ggggatgggg agcgttttcc gaagatggga
     1861 acccgagtaa aagtccatta ccaaggctcc ctgctcgacg gccgaatctt tgactcgtcg
     1921 attcaacgcg gcgagccgac ggagtttcgg ataggagaag tgatcgaggg atggaacgag
     1981 gcgcttgtga cgatgagcgc aggagaagaa cggactctca tcatccctcc ggagcttggc
     2041 tacggcgaga tgggatatcc gggaatcatt cctccgaact cctacctgat cttcgaggtt
     2101 cacctccttt ccttctaaag gcggtttgat gtggcgaaaa gaagcggcga tctgcaattc
     2161 gtatgttcaa cctgcggcag gaccgaagcg aagtggctcg gtcgctgccc cgattgcggc
     2221 tcatggaaca gttttgaaga agaactcatc gtcgctccct cgaagggagc aaagccgctc
     2281 agcacggatg aagagccggt aaggttggag gatgttgtcg tagatcctca atttcgctat
     2341 gccagcggca tcagcgaatt ggaccgggtc cttggcggag gagtgatgaa aggctcctcg
     2401 gtgctcatcg gaggcgagcc ggggatcggg aagtccacgc tgatgctcca gcttcttcaa
     2461 agcacccgga atacctccaa ggtgctctat gtgagcggag aagaatcacc ttcgcaggtg
     2521 aaaatgcggg cccagcgcct cggcttgcac ctaagcaaga tcaatatctt ctgtgatacc
     2581 aggctggaag tgcttgaaaa ggttctgcca agttcaaaag cttcactggt ggtgatagat
     2641 tccctccaaa cgctcagcag tgatacgctg ccttcgccgg caggatcggt gaaccagatc
     2701 agacactgct ccaacgcgct ggtcagcttg gcgaagcgat tggggatcag tttattcttt
     2761 gtcgcccacg ttaccaagga agggacgatt gccggaccca agatcattga tcaccttgtt
     2821 gatacggttc tgtacttcga tcaactgcct tccggaatcc gccttattcg ggcggcgaaa
     2881 aaccgctttg gaagcgtcga tgagatcggc ctgttctcga tggatgaaaa aggtcttcat
     2941 ccggtagcga atcccgcctc attttttctt tccgaccgag cggaggggcc cctgcccccg
     3001 ggagtagcct atactgcagt ggcggaagga acccgttcct ttctggtgga ggtccaagct
     3061 cttaccgttc ccgccaaaag cggcttcagc agaatcttct ccgaccggat cgacagcgcc
     3121 cgggttaccc gaatcgccgc cgtgctggag cggcacgccg gccttagact cagcgatcag
     3181 gacatctacg tgaatgtcgg cggagggatg aaggtggggg aggtcgccgt cgaactggct
     3241 cttgctctcg ccctgtattc ggcgcttacc cggacgagtc tgccaaagga tttggtgagc
     3301 ttcggcgagc tgagcctctc gggagagatt cgtccggtcg ggttcattca gcgccgggag
     3361 aagacggcca aagagctggg ctttacccga tccttagtta gtaaggcctc caaacaatct
     3421 tctttgactc aatatggagc ccgaacgatt cgggatgccc tcgatgccgt ccgagagatg
     3481 cattgataca ataattagcg agcctaacaa ttataaatag ttatacctaa acatattaaa
     3541 aactgagata tatggttgaa catatcgaag atcctattag ttattacccc taactatagt
     3601 gtttgttctt agagctgggg gagtgtcgga acgatgttcg
//
LOCUS       DAOPUZ010000069         3601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_26277_length_3601_cov_2.682177, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000069 DAOPUZ010000000
VERSION     DAOPUZ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26277_length_3601_cov_2.682177"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /gene="ligA"
                     /locus_tag="PKN79_01945"
     CDS             complement(<1..1149)
                     /gene="ligA"
                     /locus_tag="PKN79_01945"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNZ94555.1"
                     /translation="MDTIEREITKLRSELEAYQRAYYVDSRPLVDDSEYDRKFDRLLE
                     LERRYPEFASSTSPTVRVGSDISADFPEVEHTIAVLSLDKAYTEEELLSWIGKLEKNA
                     SDELGIIVEEKIDGVSIVLYYEDGILDRAVTRGNGVVGNDVSANVKTIPTIPLSLPEA
                     VTVAVRGEIYLPKTEFETLNKTLETPYANPRNLASGTLRRIKSGEVAKIPLRIFAYEG
                     FWSVDQPTTHLEVLAKLKGYGFTINEHFAYFTKDAIDAGSALAAAGLSATSGSWEEIG
                     SFIHERTERRNSLSYEIDGLVAKVDSIAYRETLGSTGHHPRWALAYKFESPQAVTTLL
                     GIDVQVGRTGRVTPVARVKPAQVAGSTISNITLHNQEYINGLELGIGDI"
     gene            1234..3183
                     /locus_tag="PKN79_01950"
     CDS             1234..3183
                     /locus_tag="PKN79_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNZ94556.1"
                     /translation="MAEKRPTMGRLTPLQGVLITSRIARGRFDRIVVPEADPSFVIVA
                     PRDIPGSNRLRVLDAQIPLLSSGAITYRDQPLLALFGPNRESALVKAKGISVTYVEDE
                     PVDSLTVTPYSYTFGNLEEALKEENLNILERSYAFHSKTKKRLTYNRVSVTFDGETLH
                     IEAPTQWPSHVRESVAEVTGFSKKNVIIHRQPFYAPHDEMLILPAQLAALCSVAAIKS
                     GRDTEILYEMEAAPPRYEITRKTYYQSSGKPQAEEIRVVVDQGCASFFREELVNHLYT
                     GLIPLYPVTALTIHVEVRTTNNRPAHFYGGLGYSEALASSEAHYSELAKLTGYNPLSW
                     RMKFASENPSSGAMIRTDKYTKLKELASTVSKRADFTRKNAAYEMQRQMRIRHSTFFK
                     YGRGNGLALAPGIGGFSREYKGTPTQSVQVTLMPNDHVVINTSFYSIGNSAEIWKQIV
                     SDELGVSKKDISFVEDGNELLDSGPSVLNANSGRMPRHILRACQLIKEKRFVEPLPIS
                     ETVTFFRHPSAKDSLFFSNSWVAIILELEISPLALVPIIRSIWVSITTPKVYERAAYI
                     SKVRHTIVTTLLEAGAQLSQSRGFTIDIQLEEEGEGMPSSMTAAVKGAVTAALLTALE
                     QAVGSSVERIPVDGPTILNLLRSKH"
     gene            3180..>3601
                     /locus_tag="PKN79_01955"
     CDS             3180..>3601
                     /locus_tag="PKN79_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNZ94557.1"
                     /translation="MKLECTIDSKSLTLHVNSTKPLSLMLIEDVGVKSMLSHCQGNAC
                     GNCIVLVNDVAVLSCIVPAFRLKDAKIRTFETFHRTRLCRDIERAYRTSGNKPCPDCY
                     ASKTLLIESILIELMSSTRSREEGMVDEKAIMQELSLNT"
BASE COUNT          803 a         1085 c          978 g          735 t
ORIGIN      
        1 gatgtcaccg attccgagct caaggccgtt gatgtactcc tgattatgaa gggtgatgtt
       61 gctgatcgtc gaacctgcca cctgagccgg cttgaccctt gccacggggg tcacccgccc
      121 ggtgcggccc acctggacat cgatgcccag aagagtggta accgcctggg gcgattcgaa
      181 cttgtaggcg agcgcccacc tcgggtggtg gccggtggag ccgagcgtct cccgataggc
      241 gatcgaatcg actttcgcga caaggccgtc gatctcgtag gaaagcgagt ttcggcgctc
      301 ggtccgttcg tggataaagg agccgatctc ttcccatgaa ccggaggttg cgctcagacc
      361 ggctgctgca agagcgcttc cggcatcgat cgcgtccttg gtgaaatatg caaaatgctc
      421 gttgatcgtg aagccgtatc ctttgagttt tgccaacact tccaaatggg tggtcggctg
      481 atcgacgctc cagaaccctt cgtaggcgaa gatccgcagc gggatctttg caacttcgcc
      541 ggacttgatc cttctcagcg ttccgctggc gaggtttcgg gggttggcat acggggtttc
      601 aagggtcttg ttgagggttt caaactccgt cttgggaaga tagatctcac cccgcaccgc
      661 caccgtcacc gcctcgggga gggagagcgg aatcgtcgga atcgttttca cattcgcact
      721 cacatcgttg cccaccactc cgtttccccg ggtcaccgcc cgatcgagaa tcccgtcttc
      781 gtaatacagg acgatcgaga ccccgtcgat cttctcctcg acgatgatgc ccagttcatc
      841 ggaggcgttc ttttcaagct tgccgatcca tgaaaggagt tcctcttcgg tgtacgcttt
      901 atcgaggctg agaacggcga tcgtatgttc cacttcggga aaatcagcgg atatgtcgct
      961 gccgacccgg acggtcggag acgtggagga agcgaactcg gggtatcgcc gctccaattc
     1021 gagcaggcgg tcgaacttcc gatcgtactc cgaatcatcc accagcggcc ggctgtcgac
     1081 gtagtacgcc cgttgatatg cctcaagctc cgaccggagc tttgtaatct cacgctctat
     1141 ggtatccatg cctccgattg tatacgccgc gttcaagaga ggcaattgcc ccaatgcttc
     1201 aggcgtggta tcgtttggaa tcagaggaag attatggcgg aaaagcgacc aacaatgggc
     1261 aggctcacac cccttcaagg agttctgatc accagtcgaa tcgctcgggg gcggttcgac
     1321 aggatcgtcg ttcccgaagc ggatccctcc tttgtcatcg tcgccccccg ggacatcccc
     1381 ggctccaatc gcctgcgcgt cctcgatgcc cagattcctt tgctcagcag cggagcgatc
     1441 acctaccgcg atcaaccgct gctcgccctc ttcggaccca atcgggaaag cgcccttgtc
     1501 aaagccaagg gaatctccgt cacctacgtc gaagatgaac cggtggactc cttgaccgtg
     1561 acgccatact cctacacgtt cgggaatctg gaggaggcgc tgaaagagga aaacctcaac
     1621 attcttgaac gttcctacgc cttccactcc aaaaccaaaa aacgccttac gtacaaccgc
     1681 gtatccgtaa cgtttgacgg agaaaccctc cacatcgagg cgcccaccca gtggccctcc
     1741 cacgtccgtg aaagcgtggc ggaggttacc ggcttttcca aaaagaacgt catcatccac
     1801 cgccaaccct tttatgcgcc ccatgacgag atgctgattc tccccgccca gctcgcagcg
     1861 ctgtgcagcg ttgcggcgat aaaaagcgga cgggacacgg aaatcctcta tgagatggag
     1921 gccgctccgc cgcgctacga aatcactcga aagacctact accaaagcag cgggaaaccc
     1981 caggcggagg agatccgggt cgtcgtcgac cagggatgcg cttccttctt ccgggaggag
     2041 ctggtaaacc atctctatac cggcctgatc cccctctacc cggtcaccgc gctgacgatt
     2101 catgtggagg ttcgtaccac gaacaaccgg ccggcccact tctacggggg gcttggctac
     2161 agcgaggcgc tggccagcag cgaagcccac tacagtgaac tggcgaagct gaccggatac
     2221 aatccgctct catggaggat gaaattcgct tcggagaacc cttcgtccgg cgcaatgatt
     2281 cgaacggaca aatatacaaa actgaaagag ctcgcctcca cggtgagcaa gcgagccgac
     2341 ttcacccgaa aaaacgccgc ctatgagatg cagcggcaga tgcgaatccg ccactccacg
     2401 tttttcaagt acggtcgggg aaacggtctt gcacttgctc cgggaatcgg cgggttcagc
     2461 cgggagtaca aggggacgcc gacgcaaagc gttcaagtga ccctcatgcc gaacgaccat
     2521 gtcgtcatca acacctcatt ctacagcatc ggcaattccg ccgagatctg gaagcagatc
     2581 gtcagcgatg agctgggtgt atcaaagaaa gacatctcct tcgtcgaaga cggcaacgaa
     2641 ttgctcgaca gcggtccttc ggtcctcaac gcaaacagcg gaagaatgcc tcggcacatc
     2701 ctgcgagcgt gccagctcat caaggaaaaa cggttcgtcg agccgcttcc gatcagcgag
     2761 accgttacct tcttccgcca tccttcggca aaggattccc tcttcttctc gaactcgtgg
     2821 gtcgccatca ttcttgagct tgaaatctca cccctcgctc tggtgccgat catccgctcc
     2881 atctgggtct ccatcaccac cccgaaggtg tacgaaagag ccgcctatat cagcaaggtt
     2941 cgccacacga tcgtaacgac gctgcttgaa gcgggagctc aactctccca gtcgaggggg
     3001 tttacgatcg atatccaact tgaggaggaa ggagaaggca tgccctcctc gatgaccgcc
     3061 gccgtcaaag gggcggtgac cgccgccctg ctcaccgcct tggagcaagc ggtcggatca
     3121 agcgtcgaac ggattccggt ggacggaccg acgattttga accttctaag gagtaaacat
     3181 tgaaactgga atgcaccatc gactccaagt cattgacatt gcacgtcaat tccaccaagc
     3241 cgctttcgtt gatgctcatc gaagatgtgg gggtcaaatc gatgctcagc cactgtcaag
     3301 gcaacgcgtg cggtaactgc atcgttctgg tgaacgatgt ggcggtctta agctgcatcg
     3361 ttcccgcgtt ccgcctgaag gatgcgaaga ttcgaacctt cgagacattt catcgcaccc
     3421 gtctctgccg ggacattgaa cgggcctacc ggacaagcgg aaacaagccc tgtcccgatt
     3481 gctatgcatc aaagacattg ctcatcgaat cgatcctcat cgaattgatg agcagcaccc
     3541 gcagccggga ggaggggatg gtggatgaga aagcgatcat gcaggagctc agcctgaaca
     3601 c
//
LOCUS       DAOPUZ010000070         3598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_26319_length_3598_cov_3.069715, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000070 DAOPUZ010000000
VERSION     DAOPUZ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26319_length_3598_cov_3.069715"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PKN79_01960"
     CDS             complement(<1..356)
                     /locus_tag="PKN79_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94558.1"
                     /translation="MKTWQPYALVILGILLIFSGSLFAAIRYDSTEISLGATHTFVEG
                     DPAYIKSIGIAVQPSFASKAHPNDLFFASLRYFYNFDRYSKLFDPTRTDFLHTLALSL
                     GIEQKRGMTSIALGLGG"
     gene            624..1997
                     /locus_tag="PKN79_01965"
     CDS             624..1997
                     /locus_tag="PKN79_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNZ94559.1"
                     /translation="MADEKKKTPVKKEAEVKKEPEVKKEAPVKKEEAPVKKGVLSWYF
                     NFNLLYRILIGLVLGALLGIILGEKMLWIAPLGGLFVRLLKMIMMPIIFSTLVVGAAS
                     ISPAKLGKVGLRVVLFYLVTSAFAVFVGLVMGSIFRPSAEMGALVAGAAGKAAAKTPV
                     STVLLNIIPTSVAQAVVNETVLQVIFFAIIFGIGISYLNISKDKRLKTAGETLIVVFD
                     GVAEVMMRMVTGIMQYAPIGVLALVAEVFAKQGSKVAGSLAVVTIACFLGYAIHVVLV
                     YGGMLMINKLSIKRFFNDAKDPFITAFVTRSSNGTLPVTIEAAKRLGIPRDVYSFSLP
                     LGATINMDGTAIYQGVCAIFISLTVTGANFTLGQMGMIIVTATLASIGTAGVPGAGAI
                     MLLMVLESVGLPVVEGSAVAGAYAMILGIDAILDMGRTALNVTGDLVCTTVVSKNMGL
                     LDQKAWE"
     gene            complement(2045..2428)
                     /locus_tag="PKN79_01970"
     CDS             complement(2045..2428)
                     /locus_tag="PKN79_01970"
                     /inference="COORDINATES: protein motif:HMM:NF025825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNZ94560.1"
                     /translation="MASIGSIRERMQELYVYDLYGFRSENKALAGVLYPHEKINSFAV
                     GLHEGLKKMLVVTTHRLIIIRSSFGKTPDVQAVKRELIEEPSYTKRFFNSSLSFKLHG
                     ETFTFTMVSRRVLELFVWAVEQPLE"
     gene            2504..3259
                     /locus_tag="PKN79_01975"
     CDS             2504..3259
                     /locus_tag="PKN79_01975"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HNZ94561.1"
                     /translation="MNAIGIIGGVGPMAGADAAQKIFENTNAATDQEHIDLYLTSTPR
                     SIGDRTKFLLEGGSNPAEGLYRNALQLIGLGASTLIVPCNTAHAPPIFDPLRKKLRDS
                     HPDITLLHMIEETAGHIAKRFPEPTTIGLLATKGTHALKTYPNALLAYPHITLIEPDR
                     ESRERVHDAIYNQRYGIKARAPVSREALTILIEEAYKLHKQGAEALILGCTELPLALT
                     RETISLPLIDPTVVLARSAILHTEPAKLKDEVE"
     gene            3256..>3598
                     /locus_tag="PKN79_01980"
     CDS             3256..>3598
                     /locus_tag="PKN79_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94562.1"
                     /translation="MITYICKQCGKTYPLSTKRYACECGSLFHLSYPKTAPAFGSLGG
                     DRSLWRYRSVLPPFEEKTIKRVTMGEGGTPVIRLEEHLYGKADYLMPTLSFKDRGAVM
                     LAALMVEIGVKR"
BASE COUNT          873 a          933 c          979 g          813 t
ORIGIN      
        1 ccgccgagac ccaaggcaat cgaggtcatt cctctcttct gctctatccc cagactcaac
       61 gcaagggtgt gaagaaaatc cgtcctggtc gggtcaaaca gcttggagta gcgatcgaag
      121 ttgtaaaagt atcggaggga ggcaaaaaac agatcattcg gatgggcttt ggaagcaaag
      181 gagggctgca cggcaatgcc gatgctctta atataggcgg gatctccttc cacgaatgtg
      241 tgcgtagcgc cgagggaaat ctccgtcgag tcatagcgga tcgcagcaaa aagggaaccg
      301 ctgaatatca gcaggattcc cagtatcaca agggcatatg gttgccatgt cttcatagaa
      361 ttcctcacat cgtaaagtat agctgacgtt cttttatggc acaagggaag cggaagggtg
      421 gagaacgtct tcagtccacg atagcataca tattcaaaat atgagcaaaa atcacaacat
      481 aacggctaaa tgtcctctaa aagagcaaat gaatcggtat aatcaaagat ttttgtttca
      541 ttctaaccgt tggtagtgta atgttatgat tagagaagct caattgttga gcgacaaact
      601 ggcatgttcc tcaggaggtt agtatggcag acgaaaagaa gaaaactcca gtcaaaaaag
      661 aagcggaagt gaaaaaagaa ccagaggtca aaaaggaggc tccggtcaag aaggaggagg
      721 ccccggtcaa aaagggtgtg ttaagttggt atttcaactt caacctgctc tatcggatcc
      781 tgattggtct cgtacttggt gcgttgcttg gaatcattct tggtgagaag atgctctgga
      841 tcgctcccct tggtggtctc tttgtccgac tgttgaagat gatcatgatg ccgatcatct
      901 tctccacact ggtcgtcggc gccgcatcca tcagcccggc gaaactgggt aaggtaggtc
      961 tcagagtcgt cctcttctac ctcgttactt cagcatttgc ggtctttgtc ggcctcgtca
     1021 tgggttccat cttccggccg agtgcggaaa tgggcgccct tgtagccggt gccgcaggaa
     1081 aagccgcggc caagactccc gtttcaacgg tgctcctcaa tatcattcct acgagtgttg
     1141 ctcaagcggt cgtgaacgaa acggtgcttc aggtgatctt ctttgcgatc atcttcggca
     1201 tcggcatctc ctatctgaac atcagcaagg ataagcgctt gaagaccgca ggtgaaacgc
     1261 tgatcgtcgt ctttgacggt gtagcggaag tcatgatgag gatggtaacg ggaatcatgc
     1321 agtatgctcc gatcggagta ttggctcttg ttgccgaagt atttgcgaag cagggctcca
     1381 aagtggccgg atcattggcg gtcgtgacga tcgcatgttt ccttggatat gcgattcacg
     1441 tcgtgctcgt ctacggcgga atgttgatga tcaacaagct ttcgatcaaa cgatttttca
     1501 acgatgcaaa agatcccttc atcaccgcat tcgttacccg ttcttccaac ggaacactgc
     1561 cggtaacgat tgaagcggca aagagacttg gaattcccag agacgtatat tcattctccc
     1621 ttcccctcgg ggcgacgatc aacatggacg gaacggcgat ctatcaaggt gtctgcgcga
     1681 tcttcatcag cttgacggta accggcgcca acttcacctt gggtcagatg ggtatgatca
     1741 tcgtaacggc gaccctcgca tcgatcggaa cggccggtgt ccccggagcc ggtgcgatca
     1801 tgcttctgat ggttctcgaa tccgtcggac ttccggtcgt cgaaggatcc gcagtcgccg
     1861 gcgcatatgc gatgattctc ggaatcgatg cgatcctcga catgggtcga accgccctga
     1921 acgtaaccgg agacttggtc tgtacgacgg tcgtttccaa aaacatgggt ctgttggacc
     1981 agaaagcttg ggaataaacc gttatccggt gtaaaccaat gtggggcctt cgggccccct
     2041 ttgtttattc caagggttgt tcaaccgccc atacaaagag ctccaggact cggcgcgaga
     2101 ccatggtaaa ggtgaaggtt tcaccatgaa gcttgaaaga gagcgaagaa ttgaagaacc
     2161 gcttggtgta ggacggttct tcgatcagtt cgcgcttgac cgcctggaca tcgggtgttt
     2221 tcccaaacga ggatcggatg atgatgaggc ggtgggtggt aaccacgagc atcttcttca
     2281 gcccttcatg aaggccgacg gcgaagctgt tgatcttttc atggggatag agaacaccgg
     2341 ccaacgcctt gttttcgctt cgaaacccat agaggtcata gacatagagt tcctgcatcc
     2401 gttcccgaat cgatccgatt gaagccattc ttccttcctc caaaatccac tatagcatac
     2461 aacacttctt gtagcggagg aaatggtgcg gtactctgta tgcatgaacg caatcggaat
     2521 catcggggga gtaggcccca tggcgggcgc ggatgcagcg caaaagatct ttgagaatac
     2581 caatgccgca acggaccagg agcacatcga cctgtacctc acctctacgc cgaggtcgat
     2641 cggagaccgg accaagtttc ttctcgaagg gggaagcaat cccgctgaag ggttgtaccg
     2701 aaacgccctt caattgatcg gtctgggcgc ttcaacccta atcgttccct gcaacaccgc
     2761 ccatgctcct ccgatcttcg atccgcttag gaagaaactc agggattccc atcccgatat
     2821 caccttgctg cacatgatcg aggagacggc cgggcacatc gccaagcggt ttcccgagcc
     2881 cacaacgatc ggcttgcttg cgacaaaggg cacccatgcc ttaaaaacct atccaaacgc
     2941 cctccttgcg tatccgcaca tcaccctgat cgaacctgac agggagagca gggagcgggt
     3001 acacgatgcg atttacaatc aacggtatgg aatcaaggcc cgggcgccgg tcagccggga
     3061 agcgctgacg atcctgatcg aagaggcgta caagctgcac aagcagggag ccgaagccct
     3121 gattctcggg tgcacggaac tgccgctggc gctcacaagg gagacgattt cgctgccgct
     3181 tatcgacccc accgtggtgc tcgcccgctc ggcgatcctg cataccgagc ccgccaagct
     3241 gaaggatgaa gtcgaatgat cacgtatatc tgcaagcagt gcggcaaaac ctatccgctt
     3301 tcaacaaaga gatatgcctg cgaatgcggt tcgctgttcc acctctcgta tcccaagacc
     3361 gctccggcct tcggctcctt gggaggcgat cgctctctgt ggcgctatcg gtcggtcttg
     3421 ccgccgtttg aagaaaagac gatcaagcgt gtcacgatgg gcgagggcgg gactccggtc
     3481 atccgattgg aggaacacct ctacggcaaa gcggactatc tgatgccaac cctctcgttc
     3541 aaggatcggg gggcggtgat gctcgccgcg ctcatggtgg aaatcggggt gaagcgct
//
LOCUS       DAOPUZ010000071         3587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_26427_length_3587_cov_3.359853, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000071 DAOPUZ010000000
VERSION     DAOPUZ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26427_length_3587_cov_3.359853"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2527)
                     /locus_tag="PKN79_01985"
     CDS             complement(<1..2527)
                     /locus_tag="PKN79_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3744 domain-containing protein"
                     /protein_id="HNZ94563.1"
                     /translation="MCLHHAVQVFAMAEVLISFKEFSFTYKAQSEPTLKHINLSIHRG
                     EKILIAGPSGSGKSTLGYCINALIPHAFKGKIEGNATICGLNLADGDMYAVNKRVGTV
                     MQDTDAQFVGITVAEDIAFSLENQCVEQREMHSTVAEIAHVVKMGSFLSHSPQELSGG
                     QKQRVSLAGVLVDDVDILLFDEPLANLDPATGALAIEMIDELATLHNKTVIIIEHRLE
                     EVLHRKVDRILVMQEGKLVSDTRPKELLASQTLRALGLREPLYITALRMAGCDLKRAG
                     DLSSYEGVKLDSVKEAIHDWYAVSQKDSAARERDVLLRLENLSYSYDGLVMAVEGISA
                     SIHSGEMISVLGNNGAGKSTLAQLIMGVLRQDSGSISFEGIDLAGYSAGARSDLVGFV
                     MQNPNHMISNDLVFDEVAFALRQRGYEEALIRERVLDVLGLCGLREYHHWPISALSYG
                     QKKRVTIASILVTDPKILLLDEPTSAQDYLHYTALMQFLAELNEKTGLTILFITHDMH
                     LALEYTTRSLVLSGGKLLADLPTSEVFSNPDLLSRANLKETSLYHLAKDLGIADIPLF
                     IEHFVQEERKERPPSVEVIRLPKRKLALTERKKEIKVGKKVVKEGRKFGFALSWEDTD
                     SYLHSFNGVTKIAFFGLWIGFCLLTFDLRFLLPALGFSFYLLWATKIPLRKFLPYIIG
                     ILGVIVLNAIFIFLFSPKQGVVYLGSETLLLGNEMMRYALSRETLFYLFVVCVKYLTL
                     FPVALVFVSITHPSEFASSLNRLGIGYTVSYAVSLALRYLPEVTKTYVNILRSQMARG
                     VDISKNAPLRKRLSSMARIIAPFVLFSLDRIEVITNAMVLRGFA"
     gene            complement(2537..3103)
                     /locus_tag="PKN79_01990"
     CDS             complement(2537..3103)
                     /locus_tag="PKN79_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HNZ94564.1"
                     /translation="MAKAKGLDPVRFVVIVAIGAALYGLGGMIGVPIFANTTLKPAMA
                     ILGLFAALYGPIVGFLVGFLGHWITDLFMGWGVWFTWVLGSGLVGVMIGLFKYWSKDC
                     VHEGNLPGMQILLFIVISFVANYLGYLVSALLDALLYTEPLDKVFTQQILAATTNTLM
                     IATLGTLLLYLVARRNASSRDLKKDEKA"
     gene            complement(3116..>3587)
                     /locus_tag="PKN79_01995"
     CDS             complement(3116..>3587)
                     /locus_tag="PKN79_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNZ94565.1"
                     /translation="QKSHTFFGRDVYAYNGARLASGELAFRDCGPVLEEFIRLEVEEQ
                     RREGETIIGAIDILDTRYGSLWTNINGDLLEEIGVTWGDYLHVRITKDNRIVYGYPLR
                     LCKAFTDVPKGEALMYINSLLNIAVALNQGNFASTYSIGTGEGWKITFSKYDTP"
BASE COUNT          847 a         1019 c          945 g          776 t
ORIGIN      
        1 gggcaaaccc gcggaggacc atcgcattgg taatcacttc gatccgatcc aaactgaaga
       61 gaacgaacgg ggcgatgatc cgggccattg aagaaagacg ttttctcaac ggcgcatttt
      121 tggagatatc caccccccga gccatttgag agcgaaggat gttcacatac gtcttggtta
      181 cctcgggcag atatcgaagc gccagcgaaa cggcgtagct caccgtatat ccgatcccca
      241 accggttcag ggaggaggcg aactccgaag gatgggtgat cgagacgaag acgagcgcca
      301 cgggaaagag cgtaagatat ttgacgcata cgacgaacag gtagaagagg gtttccctgc
      361 tgagggcgta gcgcatcatc tcgtttccca gaagaagggt ctccgaaccg aggtacacca
      421 ccccctgctt cggagagaag aggaagatga agatcgcgtt gaggacgatg acgccgagga
      481 ttccgatgat gtacggaagg aactttcgca aaggaatctt ggtcgcccac agcaggtaga
      541 atgaaaagcc aagggcaggc agaagaaacc gaagatcgaa cgtcagcagg cagaacccga
      601 tccaaagtcc gaaaaaggcg atcttcgtta ctccgttgaa ggaatgaagg taggaatcgg
      661 tgtcctccca gctgagagcg aaaccgaact tccgcccctc cttgacgacc ttcttgccga
      721 cctttatctc ttttttccgc tcggtgaggg caagcttccg cttcggcagc cggatgacct
      781 cgacgctcgg gggacgttct ttccgttcct cctgcacgaa atgttcgatg aaaagcggaa
      841 tatcggcgat tcccagatct ttggccaagt gatagagact tgtttctttg agattcgccc
      901 gggaaagaag atccggattg ctgaacacct cgctggtggg cagatcggca aggagctttc
      961 ccccgctgag cacgagcgag cgggtcgtat actccagcgc caaatgcata tcgtgggtga
     1021 tgaagagaat cgtaagcccg gtcttctcat tcagctcggc aaggaactgc atcaaagcgg
     1081 tgtagtgaag gtaatcctga gcgctggtcg gttcatcgag cagcaggatt ttcggatcgg
     1141 tgaccagaat cgaggcgatc gtaacccgct tcttctgtcc atagctgagc gcactgatcg
     1201 gccagtggtg atactcacgc aatccacaga gccccagcac atcaaggacc cgttccctga
     1261 tcagggcctc ctcatagcct cgttgccgga gggcgaacgc cacttcatcg aacaccagat
     1321 cgttgctgat catgtggttg gggttctgca tcacaaaccc gaccagatcg gagcgagctc
     1381 cggccgagta tccggcgagg tcaatccctt caaacgatat cgatccgctg tcttggcgta
     1441 acacgcccat gatcagctga gccaaggtgg acttgcccgc cccgttgttc cccagaaccg
     1501 aaatcatctc gcctgaatgg atcgatgcgc tgattccctc cacagccatg accagaccgt
     1561 cgtatgagta gctcaagttt tccagcctga gaagcacatc ccgctctcga gccgctgagt
     1621 ccttctgtga aaccgcatac caatcatgaa tcgcctcctt gacggaatcg agcttcactc
     1681 cttcatagga gctcaggtcg cccgccctct tcagatcgca cccggccatc ctcagcgccg
     1741 tgatgtagag cggctcgcgg aggccgaggg cgcgcagcgt ctgagatgcg aggagctcct
     1801 tgggtctcgt atcgctgacc agctttcctt cctgcatcac cagaatccga tccaccttcc
     1861 ggtgcagaac ctcctccaat cgatgttcga tgatgatgac ggtcttgttg tggagggtgg
     1921 ccaactcgtc gatcatctcg atcgcgaggg cccccgtcgc gggatccaag ttcgctagcg
     1981 gctcgtcgaa gaggaggatg tccacatcat cgaccaacac ccccgccagg ctcacccgct
     2041 gtttctgtcc gccggaaagc tcttggggcg aatgggaaag gaacgatccc atcttcacca
     2101 cgtgggcgat ttcagcaacc gtggagtgca tctcccgttg ttcgacgcat tggttttcca
     2161 aagaaaacgc aatgtcttca gcgacggtta ttcccacgaa ctgcgcatcg gtatcctgca
     2221 tcaccgttcc gacgcgtttg ttcaccgcgt acatatcccc atctgcaaga ttcaacccgc
     2281 atatcgtggc gtttccttca atttttccct tgaacgcgtg gggaatgagg gcgttgatgc
     2341 agtagccgag ggtggatttg ccgcttccgg aaggtccggc gataaggatt ttttctcccc
     2401 ggtgaatcga gagattgata tgcttgagcg tcggctcgct ttgagcctta taggtaaagg
     2461 aaaactcctt gaaggagatg agaacttcgg ccatggcgaa aacctgcact gcatggtgca
     2521 ggcacatgtg agcttcttac gctttttcgt cttttttcag atcccgactg ctggcgtttc
     2581 ggcgggcaac gaggtagagc agaagggttc caagtgtcgc gatcatcaac gtgttggtgg
     2641 tcgccgcaag aatttgttga gtaaagacct tatccaacgg ctcggtatac agcaacgcat
     2701 caagcaaggc actgaccaaa taaccgaggt agtttgcaac gaaggaaatc acaatgaaga
     2761 gaagaatctg cattccggga agattccctt catgcacgca atccttgctc caatacttga
     2821 aaagcccgat catgactccg acaagaccac ttccgaggac ccacgtgaac cacacgcccc
     2881 accccatgaa gaggtcggtg atccagtgac cgaggaatcc taccaaaaat ccaacaatcg
     2941 gtccgtagag tgcggcaaaa agtcccaaaa tcgccatcgc cggctttaag gtggtgttgg
     3001 cgaagatcgg caccccaatc atcccaccca gaccgtagag ggccgcccca atcgccacga
     3061 ttacgacaaa tcgtaccgga tccaaacctt ttgcttttgc catcttgatt cctccttatg
     3121 gcgtatcgta tttcgaaaac gttatcttcc acccctcacc cgttccgatc gaataggtag
     3181 aggcgaaatt tccctgattc aacgcaactg caatattcaa gaggctgttg atgtacatca
     3241 acgcttcgcc cttgggtacg tcggtgaagg ccttgcacag cctgagcgga tacccataga
     3301 cgattcggtt gtccttggtg atccgcacgt ggagataatc gccccatgtg acgccgatct
     3361 cctccaacaa atctccgttg atattggtcc aaagtgatcc ataccgcgta tcgagaatat
     3421 cgatcgctcc gatgatcgtc tccccctcgc ggcgctgctc ttccacttcg agccggatga
     3481 actcttcaag aaccggcccg cagtccctga aggcgagctc gcccgaggcg agccgggctc
     3541 cgttgtacgc atacacatcc cgtccgaaga acgtatggct cttttgg
//
LOCUS       DAOPUZ010000072         3558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_26725_length_3558_cov_2.881245, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000072 DAOPUZ010000000
VERSION     DAOPUZ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26725_length_3558_cov_2.881245"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKN79_02000"
     CDS             <1..442
                     /locus_tag="PKN79_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ94566.1"
                     /translation="GVLAPFYLAKLFKPHFADGASIINISSSRDRMSQMQTESYTAAK
                     GGIAALTHALAISLAGKVRVNSISPGWIDTLGATHEGPDAVQHPAGRVGNPMDIANMV
                     LFLCSDKSGFITGENICIDGGMTKQMIYHGDHGWTLDERKRLFV"
     gene            599..2284
                     /locus_tag="PKN79_02005"
     CDS             599..2284
                     /locus_tag="PKN79_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNZ94567.1"
                     /translation="MKKITVSIGSEHHSVAIGTTVEEVLAIANLISDCRTQTYKDNPY
                     IGALLNGELHSCTRSLLADCMLEPVHLFSDMGKRVYRHSISYLLCAAVSMLYPHRRLI
                     IGHALGDGYYFRFDDKLTLGIDDISTIEKTMRMLVKQDLPIEEVTMTYRAALAYFEQN
                     NFNHTVSLLSTRNDPKITLYRLQGYLDISYEPLLSRTGQMEVWELIPYQDRGMLLRYP
                     RTHNVMGLDPFVDNPKLFSVFREYKRWGSILNVQSLGQLNKLGNAHKIESFIRLAEAL
                     QQKKIVNTADMIAERSGVKAILIAGPSSSGKTTFAHKLAIQLQVLGKKTIQISMDNYY
                     LSPDRVPKDEFGKPDLEAFEALDIASFQEDLYALMNGEEVHLPRYDFKTQIRSFEKEP
                     CRLDNRTVLIIEGIHGMNPGLTNSIDSESLFKIYISALTQINLDDHNRISTTDNRILR
                     RIVRDHRTRGTDAEMTLNMWPSVHRGEDRYIFPYQSNADVMINSALDYELGVLVTYAQ
                     PLLRMVKPSAGPAYETARRLLRFLEHANPIPDTLVPSDSLLREFIGGSEFGVI"
     gene            2294..>3558
                     /locus_tag="PKN79_02010"
     CDS             2294..>3558
                     /locus_tag="PKN79_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HNZ94568.1"
                     /translation="MEVYGVKKIRKSDIERALGTAVTLYKESVTSLGECTLALVRNGK
                     KKYLIAKGSGPMFDELEGKVTDDLKICPANHANRLVLNTYLPYTKPTTNKDGRPSIGL
                     GDRLGEATPGHIKALGNKNIFPYFAQQSIRELNLTGRTFDGVIDDAAYAVFQCGYTAG
                     WGADGDHLKKEEEIKTALRSGATMITLDSSEMIDNTIAGLPEKELLVRYGNVDEKTRT
                     FYENLYKERTFTFGTLSLTLDTVSLMKDILIYGKALDYIQKIWETFPEFKANEAFLEV
                     SIDETATPTDPKSHLFIALELKRRGVLLKTLAPRFAGEFQKGIDYIGDLAQFERELII
                     HETIALAHDYRLSVHSGSDKFSIFPLLAKHIDRPFHVKTAGTNWLEAMHVVALTDPSL
                     YRRMHTHALARFKDATAFYVVTTDLSKIKP"
BASE COUNT          864 a         1015 c          899 g          780 t
ORIGIN      
        1 gggggtgctc gctccctttt atcttgcaaa gctgttcaag ccgcattttg cggacggtgc
       61 aagcatcatc aacatctctt catcccggga ccgcatgagc cagatgcaaa ccgaaagcta
      121 tacggcggcc aagggtggaa tagccgccct gacccacgcc ttggcgatca gcctggcggg
      181 aaaggtcagg gtgaattcga tctctcccgg ctggatcgac acgctgggtg cgacgcatga
      241 aggcccggat gccgtccagc acccggcagg cagagtcgga aaccccatgg atatcgcgaa
      301 catggtgctc tttctctgct ccgacaaatc cggtttcatc accggcgaga acatctgcat
      361 cgacggcggg atgaccaaac agatgattta tcacggcgat catgggtgga cgctggacga
      421 gagaaagagg ctgtttgtat aggatgcgct ataggtaaat gtcatctacc gcataaacca
      481 cggatcattt gcacatcatg acggcaacga atgtctttat cctcgatctt acctcaagat
      541 tcggacatcc caacccgccc ctcttgttgg atatgatgcg aatgattatc atgaccatat
      601 gaaaaaaatt accgtatcaa ttggttccga acatcacagc gtagcgatcg ggacaacggt
      661 cgaagaggtt ctggccatcg cgaacctgat cagcgattgc aggacccaga cgtataagga
      721 caacccctat atcggggcgt tgctgaacgg cgagctccac tcctgcaccc gcagcctcct
      781 tgccgattgt atgttggagc cggttcatct cttcagcgac atgggcaagc gggtctatcg
      841 ccactcgatc tcttacttgc tctgtgccgc cgtatcgatg ctctaccctc atcgaagatt
      901 gatcatcggc cacgccttgg gcgacggcta ctacttccgt tttgatgata aactgacctt
      961 gggtatcgat gatatttcca ccattgaaaa gacgatgcga atgctcgtta aacaggatct
     1021 cccgattgaa gaggtcacga tgacctatcg ggccgctctt gcgtatttcg aacagaacaa
     1081 cttcaatcac accgtctctc tcctgagcac ccgcaatgat cccaagatca ctctctaccg
     1141 tcttcaaggc tacttggaca tctcctacga gccgcttctt tcccgaaccg gccagatgga
     1201 ggtgtgggag ctcattccgt atcaagaccg aggaatgctt cttcgctatc cccgcaccca
     1261 caacgtcatg ggtctggatc ctttcgtgga caaccccaag ctcttttccg tctttaggga
     1321 gtacaagcgg tggggttcca ttttgaacgt gcaatccctt ggccagttga acaaactggg
     1381 caacgctcat aagatcgaat ccttcatccg cctggccgaa gcccttcagc agaagaagat
     1441 cgtgaacacc gccgacatga tcgccgagcg cagcggcgtc aaagcgatac ttatcgccgg
     1501 tccctcctcc tccgggaaaa cgaccttcgc ccataagttg gcgattcagc tccaagtctt
     1561 gggaaagaaa acgattcaga tctcgatgga caactactat ctcagtcccg accgagttcc
     1621 gaaggatgag tttgggaagc cggacttgga agcgtttgaa gcgttggaca tcgcaagttt
     1681 ccaagaggat ttatatgccc tcatgaatgg tgaggaggtg catctgcccc ggtacgattt
     1741 caagacccag atccggagct ttgaaaagga gccgtgccgg ttggacaatc ggacggtact
     1801 catcatcgaa ggaatccacg ggatgaatcc ggggctgacc aactccatcg acagcgaaag
     1861 cctgtttaaa atctatatct ccgccctcac ccagatcaac ctcgatgacc acaaccgaat
     1921 ttcgacgacg gacaaccgga tcctcagacg cattgtccgc gaccaccgga ctcgcgggac
     1981 ggacgctgag atgaccttga acatgtggcc ttcggtccac cggggagaag accggtatat
     2041 cttcccctat caatcgaacg ccgatgtgat gatcaacagt gcccttgatt atgaactcgg
     2101 tgttcttgtt acgtacgccc aaccgctctt gcgaatggtg aaaccaagcg ctggcccggc
     2161 atatgagacc gcccgacggc ttctgcgctt cctcgagcac gcaaatccaa tcccggatac
     2221 actggttccc tccgactcgc ttcttcgtga gtttatcggg ggcagtgaat tcggagtcat
     2281 ataggagtat tgtatggaag tttatggtgt caagaagatc cgaaaatcgg atatcgaacg
     2341 cgctttgggt acagcggtca ccctctataa ggaatcggtt accagcttgg gtgagtgcac
     2401 ccttgccttg gtgagaaacg gcaagaagaa gtatctgatc gcaaagggtt ccggtccgat
     2461 gtttgatgaa ctggaaggca aggtgacgga cgacctcaag atctgtcccg ccaatcacgc
     2521 caaccgcttg gtcctgaaca cctatctgcc ctacaccaaa cccaccacca acaaggacgg
     2581 ccgcccctcg atcggtttgg gcgatcgctt gggtgaagcg acccccgggc atatcaaagc
     2641 gttggggaac aagaacatct tcccctactt cgctcagcag tcgatcagag agctcaatct
     2701 gaccggcaga acctttgacg gcgtgatcga cgatgccgcc tatgcggtat tccagtgcgg
     2761 gtacaccgcc ggttggggtg ccgacggcga ccacctgaag aaggaggagg agatcaagac
     2821 cgcccttcgg agcggagcga cgatgatcac ccttgactct tcggagatga tcgataatac
     2881 catcgccggc cttcctgaaa aagaactgct tgtccgatac gggaacgtcg atgaaaaaac
     2941 gcggacgttc tatgaaaacc tctacaagga gcgcaccttc accttcggaa cactcagcct
     3001 caccctcgat accgtttcgt tgatgaagga tatcctcatc tacggtaaag cgttggatta
     3061 cattcaaaag atctgggaaa ccttccccga attcaaagcg aatgaagcgt tcctggaagt
     3121 atcgatcgac gagaccgcta cccccaccga ccccaaaagc cacctcttca tcgcccttga
     3181 gttgaagcgg cgcggggttc ttctgaaaac tctcgctccg cgctttgcgg gagaattcca
     3241 gaagggaatc gattacatcg gcgaccttgc ccaattcgaa cgggagctca tcatccacga
     3301 aacgatcgcc ttggcgcatg attatcggct ctccgtccac tccggcagtg acaagttctc
     3361 aatctttccg cttcttgcaa aacacatcga tcggcctttc cacgtaaaaa ccgccggaac
     3421 gaactggttg gaagcgatgc atgtggttgc attgaccgat ccttccctct atcgacggat
     3481 gcatacgcac gcccttgccc gcttcaagga tgcaacggcg ttctacgtgg tgacgaccga
     3541 tttgagcaag atcaagcc
//
LOCUS       DAOPUZ010000073         3549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_26814_length_3549_cov_3.268746, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000073 DAOPUZ010000000
VERSION     DAOPUZ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26814_length_3549_cov_3.268746"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1355)
                     /gene="guaA"
                     /locus_tag="PKN79_02015"
     CDS             complement(153..1355)
                     /gene="guaA"
                     /locus_tag="PKN79_02015"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNZ94569.1"
                     /translation="MYDRVAVIDYGKGDAVGLARSIRSRGIYSRIFPYTVSKERLAEC
                     KPKLIIHVTDKEFEYINLAHEISYPSTDLNDVLLSHVQANWTMERYAEDLILQIRETV
                     GRKNVLLGLSGGVDSSVCALLIHKAVGDRLKCVFVDHGLLRKDEGATVKALFKETYRI
                     NLTYVNAEEVFLSALKGVTDPEEKRKIIGRLFVDTFYEAGRKLGDISFLAQGTIYPDI
                     VESVQPGRTLAVKSHHNVGGLPEDLSWELLEPLSDLFKDEVRALGRVLGLPEVLLGRH
                     PFPGPGLGVRCIGEVTKERLDTLREVDAIFIDEIRKANLYDEIWQALATLLPVKSVGV
                     KDGKRVYEEVCTLRAVNSVDAMSASIYPFTGEFMNRTVLRILNEVPSVSRVLWDVSPK
                     PPGTVEWE"
     gene            complement(1368..1880)
                     /locus_tag="PKN79_02020"
     CDS             complement(1368..1880)
                     /locus_tag="PKN79_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNZ94570.1"
                     /translation="MNKEKHALKVAVVAVLTAVVVVFTLVVRIPTTKGYLNLCDVAIC
                     FIAFTFGPVSAFLAAGLGTAIADLISGYAQWAPISFIVHGIEGLLIALIVRKQPLSKM
                     RILLAAIGCVLTVSLGYFVLSALFISTVAVAAAEIPPNMIQAGVGVVFGLALSKAIKR
                     AYPPVSALSW"
     gene            complement(1894..2661)
                     /locus_tag="PKN79_02025"
     CDS             complement(1894..2661)
                     /locus_tag="PKN79_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNZ94571.1"
                     /translation="MTVNTYLSNTGWLYRFDPRAKIFFLVLLLGWVFLPVTLAGLGCM
                     LFLILFTALSNPGPRFVRSTFTSILPMIIFMVLFAPFNVRSGKGLITIRSFVLLTEEG
                     ALQALRLAIRFISITYLATLLFATTPMPEILLALQWYRLPYKASLIITLAFTYIPFIA
                     DGFAQIEESHRLREAGAHLERKRIGRIRSLIPTLTSSLVLALRSIPTLALSLELRGYG
                     RANKRTRYHDLSSYRRTFTDFLISGMIPVISFLVFKA"
     gene            complement(2658..>3549)
                     /locus_tag="PKN79_02030"
     CDS             complement(2658..>3549)
                     /locus_tag="PKN79_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ94572.1"
                     /translation="LDLRWREHLARFIQTTDKTILLFSSRYLAQFDRLFDHVFLLENK
                     QVRKVDPKQAATVFASLAGDTKPNPLVERTKEYAHQHTLSVSNLVVERRRFSASLTET
                     FHLQVPAFSLQSGEIVSLIGSNGSGKSSLCRVLCGLDEPLEGARLIDDETLDGSMLNR
                     SVGYLFQNPDLQIFLPTVKEELSYSLRRVKGLSDEEISKRVAECADLFELKLSHTPST
                     MSYPKRKALQAAVYYLLDRPFYILDELDTALTYHSALTMIRHLQRNGAGILIITHDWQ
                     FITRVGGRIYQIEGGRVSER"
BASE COUNT          889 a          970 c          932 g          758 t
ORIGIN      
        1 ggctttcctt cattacttga actccatttt ttaaagtaat actcttctta aattaataag
       61 cgttttactg ctaccgttgt tccattccgg cagcaacaaa atctgcgaag gctcaaaatc
      121 cttgaaaaat caaggttttt aactttcgaa atttattccc actctaccgt ccctggcggc
      181 tttggagaga catcccagag gactcggctg actgagggta cttcattcag gattcggagc
      241 actgttctgt tcatgaattc gccggtgaac gggtagattg aagcgctcat cgcatcgacg
      301 gagtttaccg cccggagggt gcagacctct tcatagaccc gtttcccatc cttcactccc
      361 accgacttca ccggaagaag cgtggcgagc gcttgccaga tttcatcgta gagattcgcc
      421 ttcctgatct catcgatgaa gatcgcatcg acctctctaa gcgtatccag ccgctctttc
      481 gtaacctccc cgatgcaccg gactcccagc cccggccccg ggaagggatg gcggccaaga
      541 aggacttcgg gcaagcccaa tacccggccc aatgcccgca cctcatcctt gaaaaggtcg
      601 ctgagcggct cgagcagctc ccagctgaga tcctcgggaa ggcctcccac gttgtggtgg
      661 ctcttcactg cgagcgttct gcccggttgg acgctttcga cgatatcggg atagatcgtg
      721 ccttgagcga gaaacgagat gtcccccagc tttctcccgg cttcgtagaa cgtatcgacg
      781 aaaagtctgc cgatgatttt ccgtttctct tcgggatcgg ttaccccttt cagagcggaa
      841 agaaacacct cttcggcatt cacatacgtc aagttgatgc gatacgtctc tttgaagagg
      901 gctttcaccg tcgccccctc atcctttctg agcaacccgt gatcgacgaa gacgcattta
      961 agccgatcac cgaccgcttt atggataagc aaggcgcaca cggaggaatc cactcccccg
     1021 ctcagaccga gaagcacgtt cttcctgccc accgtctctc tgatctgcag aatcagatcc
     1081 tcggcatacc gttccatcgt ccagttcgct tgtacgtggg aaagaagaac atcgttcagg
     1141 tcggtcgatg gatatgagat ttcatgagcc agatttatgt attcaaactc cttgtcggtt
     1201 acatggatga taagcttcgg tttgcattca gccaagcgtt cttttgaaac ggtgtaagga
     1261 aagatcctgc tgtagatccc gcgagatcgg atcgaacggg cgagaccgac ggcatcacct
     1321 tttccataat cgatgacggc tacacgatca tacatacagc tgctccttta ccatgagagg
     1381 gcggagaccg gcggatacgc ccgcttgatc gccttcgaaa gcgccagacc gaagaccacc
     1441 cctacccccg cctgaatcat gttgggagga atttccgccg cagcgaccgc cacggtggaa
     1501 ataaaaagag ctgaaagaac aaaatacccc agactgacag tgagaacaca cccgatcgcc
     1561 gcaaggagta tgcgcatctt cgacaacggc tgcttcctga cgatcagggc gatgagaagt
     1621 ccttcgatgc catgaacgat gaaagagatc ggagcccact gggcatatcc gctgataaga
     1681 tcggcgatcg ccgtgccgag accggcggcc aggaaagcgg agaccggacc gaaggtgaag
     1741 gcgatgaaac agatcgccac gtcacagagg ttcaggtacc ccttcgtggt cggaatcctg
     1801 accaccagcg taaacaccac caccacggcg gtcaacaccg caaccaccgc aaccttgagc
     1861 gcgtgttttt ctttgttcac catgcgcctc cgatcaggcc ttgaagacaa ggaacgagat
     1921 cacgggaatc ataccggaaa tcaaaaaatc ggtaaaggtt cggcgatagg aactcaaatc
     1981 gtggtagcgg gttcgtttgt tcgcccttcc atatcccctg agctccagac tgagggcgag
     2041 cgtgggaatt gaacgcaggg cgaggacgag actgctggtg agggtcggta tgagggagcg
     2101 aatcctcccg atccgctttc gctcgagatg ggctcccgct tcccgaagcc gatgggactc
     2161 ctcgatctgc gcaaacccgt cggcgatgaa gggaatgtac gtgaacgcca aggtgatgat
     2221 cagggaagcc ttatagggaa gccgatacca ctgaagcgcc agaaggatct cgggcatcgg
     2281 ggtggtggcg aacagcaagg tggcgaggta ggtgatggaa atgaagcgga tggcaagccg
     2341 caaagcctga agagccccct cttcggtgag caaaacgaag gaacggatcg taatcaaccc
     2401 ttttccgctg cggacattga acggcgcaaa gagcaccatg aagataatca tcggaaggat
     2461 cgaggtgaac gtcgaacgaa cgaagcgggg accggggtta ctcaacgcgg tgaacaggat
     2521 caaaaacagc atgcagccca gaccggcaag cgtaacgggc aggaagaccc acccaagcaa
     2581 cagaacgagg aagaagatct ttgcccgcgg atcgaaccga tacaaccagc cggtgttcga
     2641 aaggtatgtg ttcacggtca tcgctcgctc accctccctc cttcgatctg atatatcctt
     2701 ccgcctacgc gggttatgaa ctgccaatcg tgggtaataa tcaaaattcc tgcaccattt
     2761 cgctgcaggt ggcgaatcat tgtcaaagcg ctgtggtagg tcagcgcggt atccaattcg
     2821 tcaagaatat agaaaggccg gtcaagcaga taatataccg ccgcctgaag ggctttccgc
     2881 ttcggatagc tcatcgtcga cggggtgtgg gaaagcttca attcaaaaag atcggcacat
     2941 tcagccactc tcttggaaat ctcttcatca ctgagaccct tgacccgcct caatgaatag
     3001 gaaagctcct ctttgacggt gggaagaaaa atctggaggt cggggttctg aaaaagatac
     3061 ccgacgcttc gattcagcat cgaaccgtcc aacgtctcat cgtcgataag ccttgctcct
     3121 tccaacggct cgtcaagacc gcacaggact cggcagagcg agcttttccc gcttccgttc
     3181 gaaccgatca agcttacgat ctctccgctc tgtaaggaga aggccggcac ctgcagatga
     3241 aacgtttctg ttaatgaagc ggaaaagcgc cggcgctcca cgacgagatt cgaaacgctc
     3301 aacgtatgtt gatgagcata ttctttggtt cgttctacaa gggggtttgg cttcgtgtct
     3361 ccggcaagag acgcgaacac cgttgcagct tgtttcggat ctaccttgcg aacctgttta
     3421 ttttcaagca gaaacacatg atcaaacaat cgatcgaact gggcaagata gcgggaagag
     3481 aagaggagaa tcgttttatc ggtggtttga atgaaccgtg ccaggtgttc ccgccatcgc
     3541 aggtcgaga
//
LOCUS       DAOPUZ010000074         3536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_26963_length_3536_cov_2.884803, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000074 DAOPUZ010000000
VERSION     DAOPUZ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26963_length_3536_cov_2.884803"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..530
                     /locus_tag="PKN79_02035"
                     /pseudo
     CDS             3..530
                     /locus_tag="PKN79_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
     gene            653..1900
                     /locus_tag="PKN79_02040"
     CDS             653..1900
                     /locus_tag="PKN79_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNZ94573.1"
                     /translation="MKTWISYLAATAFGLAATLVFGESSFFQQATYTVTAVLVQLGGF
                     ILIPLVFFSFSAGIASLRKDGKGCTFARTTVFWSVFSTLLLVLSAAAVFRFLPLPFPA
                     SSSAGTDPGTFSSVGLQSFSTFLGHLTIVNPFYTLVKADDFLLPVIIIAFFLGWFLKP
                     NVEIIRPAYVVMNSFSETFFRLARAWAQCGAIFLFFISSHWLISVAKEGTVFVMLPFL
                     MILGLGVLLALFVILPLLLAIFSLFSVNPYRILYRMLAPALAGMFTGSIFFTTPATMS
                     LARQNLSVQKRVSGTAIPIYTLIGRGGSALVSTLATLTMIYAATGSVPEDMILLTIAL
                     ASALFSFVSPLYVGFEVFFITTLTLNFLKIDLYGAHMSMVALMPILNGVGILIDTYVA
                     SFGAAYTCSRMGVLVKTPHRDIV"
     gene            1897..2658
                     /locus_tag="PKN79_02045"
     CDS             1897..2658
                     /locus_tag="PKN79_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94574.1"
                     /translation="MSKGVRFTFNLIQGLVTMALSFVVIVTLIASQDGHLVLEARAHL
                     WPALIHIWITFLVSLLLATRYRANLGSEAELLPVLFLTIVLANIKILPLHQAITGTFL
                     LDGGVLTFIYHGSLLYTSFLFLIGSLLFLRLSPSRIGFITFVGAAGSALLALLVPSSP
                     NDPSFLLEAGVSDSLFLSICITLTVVAALSYLVIALGEHTKRDTRFKTVAFLLLTVGN
                     AFVSISQRTFYNIIGLIIYGAGATILVLASRSYHI"
     gene            complement(2655..>3536)
                     /locus_tag="PKN79_02050"
     CDS             complement(2655..>3536)
                     /locus_tag="PKN79_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNZ94575.1"
                     /translation="MEKELDEAMRSRIWEAKEAVEAFIEETKQLPFQYFPIQALKVAE
                     EHPVTIFDHLFIDEAQDATVMTLTALKRFVSKSIILASDGEQAIYQPGFSFTQAGIDV
                     SDNRVILQENYRSTLQIHRLAQRFERRSESEQSRALRDGPNPELHRGRDGSGLQKLVV
                     ERAMLFIERLEYEEENICIMCPSSSCYPELEQALTKAGISSVRVNDERFTFGEEYHGH
                     LRLSTLHSAKGLDIPVVLLYLPFLPRVNESYDESVNNELIRKLLYVTMTRAIDHLNVF
                     TLVSPKEEIIRELIAAF"
BASE COUNT          672 a         1066 c          889 g          909 t
ORIGIN      
        1 aagtgtacaa ccgggaagga catccccttc cttcaggatg ggcggtcgat ccgaggggag
       61 tcgtaacatc cgatgccgct caactccttg atgacatgct cttccaacga ggcggcgggc
      121 ttctaccgct tggcggagcc ggggaggagc tcggaggaca caaaggctat ggcttggccg
      181 tccttgtcga tatgatgacg gcgatcctaa gcggcggcac gttcggaaaa tccgtgtaag
      241 acagcgaagc gacgagcgcc cgggtctgcc atttcttcgg tgcgatccgc ctcgacctgt
      301 tccgcgatcc ggatgagctc aaaaacgatg tcgatgcgct cttatccgaa ctcaatgaaa
      361 cacccgtcgc cgaaggacac aagcgggtgt tgtgggcggg gcagaaggag caggaagcgg
      421 aggcccgctc tgatgtggag ggtgttccct tgtcagcgaa cgtcgtttca caattgaaga
      481 gcatcggaaa agaattgggc atcgtgttcc ccctccctct ttccaaatga catcccttcc
      541 tgtttaggtg tttcttttga tggaattcat cagctgattt gatgattcct tccgacaaac
      601 acttcctttc ctttgattga cttgctacct ttgatgatgt accctaattt gtatgaaaac
      661 gtggattagt tacttagcgg caacagcgtt cgggctagcg gcaaccttgg tcttcggtga
      721 gtcctctttc ttccagcaag caacgtatac cgtcaccgcc gtgctggtcc aacttggcgg
      781 gttcatcctc attcctttgg tttttttcag cttctccgcc ggcatcgctt ccttgcggaa
      841 agacggcaaa ggctgcacgt ttgctcgaac caccgtgttc tggagtgttt tttccaccct
      901 gctccttgtc ttgagcgcag cggcggtctt ccgcttcctg cccttgccgt tccccgcctc
      961 ctcttcggcg ggcacggacc ccggcacctt ttcaagtgtc ggattgcaat cgttctccac
     1021 ctttctggga caccttacga tcgtcaaccc cttctacacg ctggtgaaag cggatgattt
     1081 tctcctgccg gtgatcatca tcgccttctt cctcgggtgg tttttaaagc cgaacgtcga
     1141 gatcatccgt cccgcctatg tcgtcatgaa ctccttcagc gagaccttct tccggctcgc
     1201 cagagcgtgg gcccaatgtg gggcgatctt tctcttcttc atctcttccc attggctgat
     1261 ctccgtcgcc aaggaaggca ccgtcttcgt gatgttgccg ttcttgatga tcttgggtct
     1321 tggggtgctt ctcgccctct tcgtcattct gccgcttctt cttgcgatct tctccctctt
     1381 ttcggtgaac ccttatcgga ttctgtaccg gatgctcgct cccgcccttg ccgggatgtt
     1441 caccggctcg atcttcttca ccaccccggc cacgatgagc ctggcaagac aaaacctcag
     1501 tgtccagaag cgggtcagcg gaacggcgat tccgatctat accttgatcg gcaggggcgg
     1561 ctccgcgctc gtctcgaccc tcgcgacgct gacgatgatc tatgcggcaa cgggaagcgt
     1621 gcccgaagac atgattcttc tcaccatcgc cctcgcttcg gcgcttttct ccttcgtcag
     1681 tcctttgtac gtggggtttg aggtcttctt catcaccacc ctcaccctca acttcctcaa
     1741 gatcgatctc tacggagctc acatgtcgat ggttgcattg atgccgattc tcaacggtgt
     1801 cggcattctc atcgatacat acgtcgcctc cttcggagcg gcgtacacct gcagtcgaat
     1861 gggtgttctg gtaaagactc ctcatcgtga catcgtatga gcaaaggggt ccggttcacg
     1921 ttcaacctca ttcagggcct ggttaccatg gccctgagtt ttgtcgtaat cgtcaccctc
     1981 atcgcttcac aggatggaca ccttgtcctt gaggctcgcg cccacctctg gccggcgttg
     2041 attcatatat ggatcacctt ccttgtctcc ctgcttctgg ctacccgata tcgggcgaac
     2101 ctcggcagcg aagcggagct cctgccggtg ctctttctca cgatcgttct tgcgaatatt
     2161 aaaatccttc cccttcatca ggcgattacc ggaacgtttc ttctggacgg aggagtcctg
     2221 accttcatct accatggaag cctgctgtat acctcgttcc tgtttttgat cgggtcgctg
     2281 ctgtttctac ggttaagccc ctcgcgcatc ggtttcataa ccttcgtggg ggcggcaggc
     2341 tccgcgcttc tggccctgct ggttcccagc agccccaacg acccctcctt cctcctggag
     2401 gcaggagtaa gcgattcgct cttcctgtcg atctgcatca ccctcaccgt cgtagctgcc
     2461 ctatcctatc tcgtgatcgc gctgggcgaa cacaccaagc gggatacccg cttcaagacc
     2521 gtcgccttcc tactccttac cgtaggcaac gccttcgtct cgatcagcca acggaccttc
     2581 tacaacatca tcggcctcat tatctatgga gcgggggcga caatcctcgt gctggcatcg
     2641 cgttcatacc atatctaaaa cgcggcgatc aactccctga tgatctcctc tttcggactg
     2701 acaagcgtaa agacgttgag atgatcgatc gcccgggtca tcgttacata caggagcttt
     2761 cgaatcaatt cattgttgac cgattcatca taggattcat tcactcgggg gagaaacggc
     2821 agatagagca gcacgacggg aatatcgaga cctttggccg aatgcagggt ggagaggcga
     2881 agatgcccgt ggtactcctc accaaacgtg aagcgctcat cattgactcg gacgcttgaa
     2941 atccccgcct tcgtcaaagc ctgctcaagc tccggataac aggagctcga ggggcacatg
     3001 atacaaatgt tttcttcctc gtactcaagc cgttcgataa acagcattgc ccgctcgacg
     3061 accaacttct gcaaccccga tccatcacga ccccggtgaa gttcggggtt cggaccatcg
     3121 cgcagcgctc tcgattgctc gctttcgctt cgccgctcaa accgttgggc aagccggtgg
     3181 atctgcaacg tggagcgata attctcttgg aggattaccc gattgtcgga tacatcgatc
     3241 cccgcttgag tgaaggaaaa gcccggctga tagatcgcct gctccccgtc gctggcaaga
     3301 atgatcgatt tggaaacgaa ccgcttcagg gcggtgagcg tcatgacggt ggcatcttgc
     3361 gcctcatcga taaacaagtg atcgaagatc gtaacgggat gctcctcggc gaccttcaac
     3421 gcttggatcg ggaaatattg aaaaggaagc tgtttcgtct cctcgatgaa cgcttcgaca
     3481 gcctccttcg cctcccagat ccggcttcgc atcgcttcat cgagctcttt ctccat
//
LOCUS       DAOPUZ010000075         3529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_27025_length_3529_cov_4.094128, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000075 DAOPUZ010000000
VERSION     DAOPUZ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27025_length_3529_cov_4.094128"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKN79_02055"
     CDS             <1..352
                     /locus_tag="PKN79_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HNZ94576.1"
                     /translation="QVHKRGLTLLSGPGNDIVSTSALTAAGAHIILFTTGRGTPLGSP
                     VPTVKIASNTALAEKKPHWIDFDAERLLREEQDVVVDGFIDMMRETAGGKKLAKNEEH
                     GYREISIFKDGVVL"
     gene            376..1778
                     /gene="uxaC"
                     /locus_tag="PKN79_02060"
                     /pseudo
     CDS             376..1778
                     /gene="uxaC"
                     /locus_tag="PKN79_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            1781..2545
                     /locus_tag="PKN79_02065"
     CDS             1781..2545
                     /locus_tag="PKN79_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNZ94577.1"
                     /translation="MSEQQGTSGVIRTITILETLSRHQSINLESLAKETKLPKATLLR
                     FLSTLVNLGYVYRDPHDLYSLTLKMFSVGSRGLEHLDLITLARPIAQHLSEELGETVH
                     MGVLEEDNAVYVLKRESSYTIRMYSRVGKTIPVYCTAIGKVLLADMEDEEIASYLERV
                     RLKAFTPNTLTSKEALLSELEEVRTLGRAMDNEEHEMGTLCIGSPIRDHSGKVIAAMS
                     VSWPLFRFDSEQAERMSGAIQEATARLSRLMGYTGG"
     gene            2691..>3529
                     /locus_tag="PKN79_02070"
     CDS             2691..>3529
                     /locus_tag="PKN79_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HNZ94578.1"
                     /translation="MNEINMYENKEIRSVWDNEKEERYFSVIDVVAVLTDSKNPRDYW
                     YRVKKRMTEDEKSELSTFCRQLKLESSDGKKYNTDVADMQGIFRIIQSIPSPKAEPFK
                     MWLAEVGKERIDEIVDPERTIDRALQTYLRKGYSREWINQRLQAIQVRKELTDTWGDH
                     GVREGVEYAILTDEISKAWSGMTTRQYKDFKNLKKENLRDNMSTLELVLTMLAEATTT
                     ELTQTLNPHGLEENKEIAKEGGTVAGNARRDIEARTGRSVLTQKNALDFSKLIEDVAD
                     KTE"
BASE COUNT          916 a          942 c          958 g          713 t
ORIGIN      
        1 tcaggtacat aaacggggac tgacccttct ttcaggaccg ggtaacgata tcgtctccac
       61 ctccgccctg acggccgccg gagcacacat catcctcttc accaccggtc gaggaacccc
      121 tctcggctcc cccgttccaa cggtgaagat cgcctccaac acggctttgg cggaaaagaa
      181 gccccattgg atcgattttg acgccgaacg gctgctccgg gaagaacagg atgttgtagt
      241 tgacggtttt attgatatga tgagagagac cgccggcggc aagaagcttg cgaagaatga
      301 agagcacggg tatcgtgaga tttcgatctt taaagacggg gtggttctgt aaccccatac
      361 aaaggagttc ctacgatgaa acagtttatg ggagatgatt tcctcttgaa aaacaagagt
      421 tccgagcggc tctaccacga gttcgcgaag gatcagccga tctacgatta tcactgtcac
      481 ttgataccgg gtgaactggc gacgaacaaa cgcttcacca cgattaccga tatgtggctg
      541 ggcggggacc actacaagtg gcgagcgatg cgggccaacg gaattcccga ggcgatcatc
      601 acgggaaagg atgccgaccc gtatgacagg ttcctcgcct gggcgaaaac ggttgaaaag
      661 ctgatgggca acccgctcta ccactggacc catctggagc tgaagcgcta cttccacatt
      721 gatgagccgt tcaacgaaaa gaatgcgaaa acggtattcg acgagacgaa ccgccagctt
      781 cagaacaacc cgaatctttc ggtcaagggg atcctcaagc agttcaaggt ctacgcaatc
      841 ggaacgacgg acgatccggc ggatgacctt gcctgccaca aggagatcgc cggccagaag
      901 gatttccccg cccgcgtcat gccttccttc cgccccgaca aggcgctcgc catcgatgct
      961 ccggccttcc gggagtatct gggcacgctc gcttcttcag cgagcatgac gatcacgagc
     1021 cttgacgatc tctcaccgcc ctgatcaagc ggctcgactt cttcgtcgag atggggtgcc
     1081 gcgcaagcga ccacgccctg gtcaccactc cggcgctctt ctggagcgag gagaaggtca
     1141 agaaggtctt cgccaaacgc ctgaacgacg aaagcctctc caaggaggag gtcgaagcgt
     1201 acaagaccta tgtgctcacc caccttgcga aggcgtacaa gaagcgggat atcgcgatgc
     1261 agctgcactt cgcggcgatc cgcaacaaca acagcatgat gttcgaaaag ctcggaccgg
     1321 acaccggcta cgacgcttcc cacgacaagg agctcgccga ggggctttcg gcgttcctca
     1381 acaacctttc caagagcggc gaggttccca agaccatcct ctacacgctc aaccccaagg
     1441 attactactc cctcggtacg ctgatgggct gctaccagga cggtcggccc ggaaagatgc
     1501 agctcggttc ggcgtggtgg ttcgccgacc acaaggacgg gatggaggag cagatgaagc
     1561 ttttgggaaa cgtcggtctt ctctcccgct tcgtcggaat gctgaccgac agccgctcct
     1621 tcctctccta ctcccggcac gagtacttcc gaaggattct ttccaatatc ttcggaacct
     1681 ggatggaaga cggtgaaatt cccaacgata tcgatctaat tggcaagatc gtcagtgata
     1741 tctcgttcaa caatgccaaa gcctattttg agggatgatg atgagtgagc aacagggaac
     1801 cagcggagta attcgaacga tcacgattct ggagacgctt tcgcgccacc agtcgatcaa
     1861 tctcgagagc cttgccaagg agacgaagct cccgaaggcg actcttctgc gttttctttc
     1921 aacgttggtg aaccttggct atgtctatcg cgacccgcac gacctctaca gtctgacgct
     1981 gaagatgttc agcgtgggtt cgcgcggctt ggagcacctg gatctgatca ccctcgcccg
     2041 cccgatcgcc cagcacctca gtgaagagct gggcgagacg gttcacatgg gagtcctcga
     2101 agaggacaat gcggtgtatg tcctcaagag ggaatcttcc tatacgatcc ggatgtacag
     2161 ccgggtggga aagaccattc ccgtctactg cacagcgatc gggaaggtgc tcctcgccga
     2221 catggaggat gaggagatcg cctcctacct cgaacgggtc agactcaaag cgttcactcc
     2281 caacaccctc accagcaagg aagccctgct ttccgaactt gaagaggtcc ggactctcgg
     2341 ccgggcgatg gacaacgaag agcacgagat ggggacgctg tgcatcggtt ctccgatccg
     2401 cgaccacagc ggaaaggtga tcgcggcgat gagcgtttcc tggccgctct tccgcttcga
     2461 ttccgaacag gcggaacgaa tgagcggggc gattcaggaa gcgactgccc gcctctcgag
     2521 actcatgggg tatacgggcg gatgatatcc tccacccccc ctctcttctc cctctgcatg
     2581 agtcggatat gctttcctta acatcggtgg gtcgctttca agcaaacaag ggattgtcac
     2641 cacatattca ggtaagatga tggcagcgtt cttgcaccgg ggaggaatga atgaacgaga
     2701 taaacatgta tgaaaacaag gaaatacgat ctgtttggga taatgaaaaa gaagaacggt
     2761 acttctctgt tatcgatgtg gttgctgttc tgactgatag caaaaaccca agggattatt
     2821 ggtatcgagt gaagaagagg atgacggaag atgaaaaatc cgagttgtcg acattttgtc
     2881 gacaactgaa attggagtct tcggatggaa agaaatacaa tactgatgtc gccgatatgc
     2941 agggaatttt ccggatcatc caatccattc cctcaccgaa agccgaaccg tttaaaatgt
     3001 ggctggcgga ggtaggaaaa gaaagaatcg atgagattgt cgatcctgaa cgtactattg
     3061 acagagctct gcagacatat cttcgaaaag gctattccag agaatggatc aatcagagac
     3121 ttcaagcgat ccaggtcaga aaagagctga cggatacgtg gggcgaccac ggcgtacggg
     3181 aaggtgtgga atatgccatc ctcaccgatg aaatttccaa agcatggtcc gggatgacga
     3241 ctcgccaata caaggatttc aaaaacctga agaaagaaaa tcttagagac aacatgtcca
     3301 cgttggagct ggttcttacc atgcttgcgg aagcgacaac cactgaattg actcaaacat
     3361 tgaaccccca tggattggaa gagaacaaag agatcgccaa agagggagga accgttgccg
     3421 gaaatgcccg aagagatatc gaagcaagga caggcaggtc cgtcctcacc caaaagaatg
     3481 cccttgattt ttccaagctt atcgaagatg ttgcggataa aacggaaga
//
LOCUS       DAOPUZ010000076         3529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_27030_length_3529_cov_2.449626, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000076 DAOPUZ010000000
VERSION     DAOPUZ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27030_length_3529_cov_2.449626"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKN79_02075"
     CDS             complement(<1..298)
                     /locus_tag="PKN79_02075"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNZ94579.1"
                     /translation="MTGYGYAEKAHSVFSLSVEIKSYNNRYLEIICNLPPFLSGYELE
                     ISNMVSEKAARGRVEVFVKVKTLKSDLQVVVDKEVVERLSEAFARIGAISGKALK"
     gene            complement(304..1671)
                     /locus_tag="PKN79_02080"
     CDS             complement(304..1671)
                     /locus_tag="PKN79_02080"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNZ94580.1"
                     /translation="MNLPFRRIYLVGIKGSGMARLALLLANGGCRIGGCDTQEDFFTG
                     RSLTEAGIAADLGFDRYNLNGTWDAVIHSAAYPPSTDILIHAKERGIPLYSYPEFLAL
                     LSTLRPTYLVIGTHGKTTTCAMADHLLGEEGGYWAIYGSTLLKRSNSSGKKSRFFLFE
                     GCEYQDHFLLYRTSGAVLLSVEYDHPDYFGDLADVHDSFRRFVDGIGQGGFFIYNNDQ
                     TGSRAIGEYAKARRKDLLVLSFGFAPESDYPIVEEEKQHYRIGGISTRLTVGTPELVI
                     DHLGALLLASMITSKNVDPIRMKELAMRLATFGGVVGRLEYMGERAGILFFDDYAHHP
                     SEIVVSLREMRTRFPSRKILVLFMPHTATRTHALLEEFAAALRGADQLILQPTYASAR
                     KDEETDPLALYALLKTDTDCTYAADDEEAVAQALMRLKEGWLCITMGAGNNRALNSRF
                     LRSVP"
     gene            complement(1741..1917)
                     /locus_tag="PKN79_02085"
     CDS             complement(1741..1917)
                     /locus_tag="PKN79_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94581.1"
                     /translation="MSKAHRGSGIRDQYKNGRGTCPKCNRTGVKVLYEATLDGAKTKV
                     CKPCNANLKKKASA"
     gene            complement(2000..3025)
                     /locus_tag="PKN79_02090"
     CDS             complement(2000..3025)
                     /locus_tag="PKN79_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94582.1"
                     /translation="MKRLVTITVIFLLVSLSLTAKSTTQTMWAFGGARFALGSDSDSF
                     LGNPALLASSDRTTRSFILSSGFEDSAESWDFEEPSVNLGVSFIAGRMAFSIMNVSSV
                     TRIKEYTYTGKRTGLFQFDWAFGCQPFYIGFTAQIEALSERSEMTFRPELIWSDYFVA
                     TNFSRYEPVDQIGSISFGVSFLLDYSWISMAVTSTKFASSETSETLNISFDSLLKSLG
                     WGMAVETPKYNERNELHLFTVRAAVDIANMGSSEERELRMGTSVTLNLLPTYDISLIL
                     GYHEQKGEATDWLGLKFAQGFQNQTLAITLNTVKILIGYEFPTAWYVNKPSPRPAKAL
                     VGLRLFL"
     gene            3100..>3529
                     /locus_tag="PKN79_02095"
     CDS             3100..>3529
                     /locus_tag="PKN79_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNZ94583.1"
                     /translation="MKALGELLRSAHIPVASTQQSFIVTSLCTHSNACKEGSIFFGFR
                     GQNSDGSLYAKEALQNGAILAIIDEEASVEDHQFIIRVKNPHQTFALMNAAFHDNPQA
                     ELKLIAVSGTDGKSSTADFLHQLLSFEGIPAGLFSTTSIDD"
BASE COUNT          791 a          979 c          950 g          809 t
ORIGIN      
        1 gcttgagggc cttgccgctg attgcgccga tccgggcaaa tgcctcgctg agccgctcga
       61 cgacctcctt gtcgacgacc acttggagat cgcttttgag ggttttgacc ttcacgaaga
      121 cctcgacgcg cccccgggcg gctttttctg aaaccatgtt ggaaatctcc aactcatagc
      181 cgcttaaaaa cggaggaagg ttgcagatga tttcaaggta tcgattgttg tacgatttga
      241 tttcaacgct cagtgaaaag actgaatgcg ctttttccgc atatccatag cctgtcatcg
      301 atttcacgga acactcctta ggaatcggct gttgagggcg cgattgttgc cagcacccat
      361 cgtaatacac aaccaccctt ctttcaaccg catcagggct tgggcgaccg cctcctcgtc
      421 gtccgccgca tatgtgcaat cggtatctgt cttgagcagg gcgtataacg ccaagggatc
      481 ggtttcctca tcttttctgg ctgaagcgta ggtcggttgg aggatgagct gatcggcgcc
      541 cctcagtgcg gcagcaaact cctcaagaag ggcgtgggtt cgggttgcgg tatggggcat
      601 gaagagcacg aggatcttcc tgctggggaa gcgggtgcgc atctccctca gggaaacgac
      661 gatttcgctc ggatgatggg cataatcgtc gaaaaagagg attccggcgc gctcacccat
      721 gtattcgagc cgcccgacga ccccgccgaa ggtcgcaagg cgcatagcga gctccttcat
      781 ccggatggga tcgacattct tgcttgtgat catcgaagca agcagaaggg ccccgagatg
      841 atcgattacc agttccggcg ttccaacggt gaggcgggtg gaaatccctc cgattcgata
      901 gtgttgtttc tcctcttcga cgatcggata atcgctttcc ggagcgaagc cgaagctgag
      961 caccagcaga tcttttcgcc tcgctttggc atactccccg atcgcccttg agcctgtttg
     1021 gtcgttattg tagataaaga agcccccctg cccgatccca tccacgaagc gcctgaagga
     1081 atcatgcacg tccgcaaggt cgccaaagta atcgggatga tcgtattcca ccgagaggag
     1141 taccgccccg cttgttcggt agagcagaaa gtggtcctga tactcacacc cttcgaagag
     1201 gaagaaacga gacttcttcc ctgaagaatt gcttcttttg agcaatgttg aaccgtagat
     1261 cgcccaatat cctccctctt cgcccagcag atggtcagcc attgcacacg tcgtcgtttt
     1321 tccgtgggtt ccgatcacca aataggtcgg ccgaagcgtg gacagcagcg ctaaaaattc
     1381 ggggtatgaa tagaggggaa tgcctcgttc ctttgcgtgg atgaggatgt ccgtcgaagg
     1441 gggataggcg gccgagtgaa tgaccgcgtc ccacgttccg ttgagattgt agcgatcgaa
     1501 gccgagatcg gcagcgatcc ccgcttccgt caggcttcgg ccggtgaaaa aatcctcttg
     1561 ggtgtcacag ccgccgatcc ggcagcctcc gtttgccaac aacagggcaa ggcgagccat
     1621 tccgctgcct ttgattccga caaggtagat ccttcgaaac ggaagattca tgaatgcaga
     1681 ataccaaaaa aaagaccgcc cgataagggg acggtccttt cgctcaagat gatctcttcc
     1741 ctaagcggaa gctttcttct tgaggttggc gttgcagggc ttgcagactt tcgtcttcgc
     1801 cccgtccaag gttgcttcat agagaacctt tacgccggtt cgattacatt tcgggcaggt
     1861 gccgcggcca ttcttgtatt gatcacggat tccgctgcct ctgtgtgctt ttgacatatt
     1921 cccttctcct atccggttct aacgtgctaa cgcttgacta tatccaccaa agcccgggag
     1981 tgtcaaggtt gacgaacccc tagaggaaca ggcgcaaacc cacgagggcc ttggcgggcc
     2041 ggggggatgg cttgttcacg taccatgcgg tcgggaactc atagccgatg agaatcttca
     2101 cggtgttcag cgtgatcgcc agcgtttgat tctggaaacc ctgtgcgaac ttcaacccga
     2161 gccaatcggt cgcttccccc ttttgttcat gatagccgag aatcagggag atgtcgtagg
     2221 tcggcagcag attgagggtt accgaggtgc ccatcctgag ctcccgttct tcgctggacc
     2281 ccatattcgc tatatccacc gccgctcgta ccgtaaatag gtggagctca ttgcgctcat
     2341 tgtatttagg agtttcaacc gccattcccc acccgaggga cttcaataac gaatcaaagg
     2401 agatgttcag cgtttcgctg gtttcggaag aggcgaattt cgtcgatgtg accgccatgc
     2461 tgatccagga gtaatcaagc aggaaggaga cgccgaagga gatgcttccg atctggtcga
     2521 ccggctcata gcggctgaag ttggtcgcaa cgaagtagtc gctccagatg agctcgggtc
     2581 ggaaggtcat ctcgctgcgt tcggacagcg cctcgatctg ggcggtgaac ccgatgtaga
     2641 acggctggca gccgaaggcc caatcgaatt ggaagaggcc cgtccgcttt cccgtgtagg
     2701 tatactcctt gatccgagtc accgaagaga cgttcatgat tgagaacgcc atccgtccgg
     2761 cgataaatga aacgcccaga ttgacgctcg gctcttcaaa atcccatgat tcggcggaat
     2821 cttcaaaacc tgatgaaagg atgaaactcc gagtcgttcg atcgctgctt gccaacaacg
     2881 cgggattccc caagaaggaa tcgctgtcgc tgccaagggc gaatcgcgca ccgccgaacg
     2941 cccacattgt ctgcgtcgtg ctcttggcgg tgagggaaag agaaacgaga agaaatatga
     3001 ctgtgatcgt caccaagcgc ttcacgaaaa gcctccttcg tcagcatatc aaaaagtttt
     3061 gcgctatgct atcgcctacc aagctggtat gatggccata tgaaagccct tggagaactc
     3121 ctccgctcag cccatattcc ggtggcatcc acacagcagt cttttatcgt cacctccttg
     3181 tgcacccatt cgaacgcgtg caaagagggt tcgatcttct tcggtttccg aggtcaaaac
     3241 agtgacgggt cattgtacgc caaggaggct ttacagaacg gtgccatcct ggcgatcatc
     3301 gatgaagaag cttcggtcga agaccatcaa ttcatcatcc gggtcaaaaa tccccaccag
     3361 accttcgcat tgatgaacgc cgccttccat gacaaccctc aggcggagtt gaagctcatc
     3421 gcggtctcgg gaacggacgg caaatcctcc accgccgatt tcctccacca gctgctctct
     3481 ttcgaaggaa taccggccgg gctcttttca acgacgtcga tcgatgacg
//
LOCUS       DAOPUZ010000077         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_27131_length_3520_cov_2.889755, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000077 DAOPUZ010000000
VERSION     DAOPUZ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27131_length_3520_cov_2.889755"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PKN79_02100"
     CDS             <1..977
                     /locus_tag="PKN79_02100"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNZ94584.1"
                     /translation="DPWVYEQGVRVVNHDCLDDDGLTYLGKTERGSEVWISSLYLQSD
                     LKILTGLVESHFMAGVSGGRKSICPGLISEMGTFLFHGAPMMGHPDSRDLLLEGNPVH
                     EESLAFAKMAGSDFIVNVTLDHAFKITGIYAGDMEAAHLAAFEMIRGYAKIPIESEAD
                     IVVTHAGFVGINHYQSAKAAFAAIGAMKENGYLISIANYTDKKDIVGSVNYKTVLAIL
                     ANHGSKALVKLLHSPDWPFTPDQWQVQKWAAAFDKIPLDHYYHYAPQIEAGREAGLPG
                     INAAKLTDKRDYTSVVKAAIEDIYRREGTRDLTILYLSDGPYAIPYVK"
     gene            complement(1068..2033)
                     /gene="pdxA"
                     /locus_tag="PKN79_02105"
     CDS             complement(1068..2033)
                     /gene="pdxA"
                     /locus_tag="PKN79_02105"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNZ94585.1"
                     /translation="MKIFGITLGDPCGIGIEVTIKALLKEPSYQREALLFGPLSVLEY
                     YNKRLKANFTFNVIETIEEFKMGMINVVDPHPVKLEDVLIGKVSALGGEVAFEAVRTA
                     IEFARRKEISSVITAPLNKEALHLAGHRFAGHTEIFAHYAKGDSYAMLLSSPKLTVIH
                     VSTHQSLRSACDAVTKKRVLEVIRLGAETMKKFGYPHPRIAVAGLNPHAGENGLFGDE
                     EIRQIIPAIEAAKKEGHDVTGPIAPDTVFLRAIKGDWDIVVVMYHDQGHIPLKLLAFD
                     DGVNITVGLDVVRTSVDHGTAFDIADKLIASEQSMLKAIELGKKL"
     gene            complement(2030..3244)
                     /locus_tag="PKN79_02110"
     CDS             complement(2030..3244)
                     /locus_tag="PKN79_02110"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HNZ94586.1"
                     /translation="MNTYLIIADDFTGSNDSALELAHRGLSTIIGFSSPKGTVDSYVL
                     DTESRNREGDEAYRMTKALIKDLDIDSYDIVLKKIDSTLRGNILEEIRAIDEKMNSDL
                     ILVAPAFSQMGRTCVGGTVFVNSVKLLDTEFAHDPLKPVTEDNLITLFASEYSTIHLS
                     VDEIRSKAFPLIKDAQVIVCDAEHEADLFETVKWARGLDKRILYVGSAGLAGALIQYD
                     RPKQPALAILASLSKASRAQLDYAKSRGVAILAVDVLSILKGGDFTPIIDQGVQILQG
                     GEDLILTLSSALDRSVFQQSIEVGKALGLKDAVVGTMIKDCLSKVSLEIIKASGVKNL
                     FLSGGETAFGLMQMMGINEVKIIGEVDIGIPITLVLDGPYQGMKIVTKAGGFGKEDAL
                     TYSLRRLEERQL"
     gene            3310..>3520
                     /locus_tag="PKN79_02115"
     CDS             3310..>3520
                     /locus_tag="PKN79_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HNZ94587.1"
                     /translation="MSLTYTRFLTDSSFTHAQTFRDPLWKDISIPTYMAEVVQSPSFI
                     KLGRIKQLGVAFHLYPGAVHTRLNHS"
BASE COUNT          787 a          967 c          891 g          875 t
ORIGIN      
        1 tcgacccatg ggtctatgaa caaggggtcc gagtggtcaa ccatgactgt ttggacgatg
       61 atggattgac ctaccttgga aagaccgagc gcggaagtga ggtgtggatc agctccctgt
      121 atcttcaaag cgacttgaag attctcaccg gcctggtgga aagccacttc atggcgggag
      181 tgagcggagg acgaaaatcc atttgtcccg gtttgatcag tgaaatgggt acgttcctgt
      241 tccatggagc cccgatgatg ggtcatcccg attcccgtga tcttcttctg gaaggaaacc
      301 cggtccacga agaatccctt gcctttgcaa aaatggcggg atcggatttc atcgtgaatg
      361 ttacgttgga tcatgcgttc aagatcacgg gaatctatgc aggagacatg gaagccgctc
      421 acctggccgc ttttgaaatg attcgggggt atgcaaaaat tcccatcgaa tccgaagccg
      481 atatcgtcgt aacccatgcg gggttcgtcg gcatcaacca ttatcagagc gccaaagctg
      541 cgttcgccgc gattggggca atgaaggaaa acgggtatct catcagtatc gccaattata
      601 ccgacaagaa agatattgtc ggatcggtaa actacaaaac ggtcctggcg attctggcga
      661 accatggatc gaaggcgctg gtgaagcttc ttcactcccc ggattggcct ttcacccccg
      721 accagtggca ggtgcagaag tgggctgccg cctttgacaa gattccactg gaccactact
      781 accactatgc tcctcaaatc gaggcgggca gggaagcggg tcttccgggc atcaatgccg
      841 ccaaacttac cgacaagcgg gattacacca gtgtcgtcaa agcggcaatc gaggatatct
      901 acaggcgcga aggaacccgc gatctgacga ttctttatct cagcgatggt ccatacgcca
      961 taccgtatgt gaagtgatcg tttacaaccg cagggggccg aacggccccc tgattctttt
     1021 ccttcccgct tccttttatc ttcttgtttc tctttttttc tttgtctcta gagcttcttt
     1081 cccaactcaa tcgccttgag catgctctgt tcactggcga tcagcttgtc ggcgatgtcg
     1141 aacgccgtgc catgatcgac actggtccgc accacgtcaa gaccgacggt gatgttcacc
     1201 ccgtcatcaa acgccagaag tttgaggggg atgtgccctt ggtcatggta catcaccacc
     1261 acgatatccc aatccccttt gatcgcccgg agaaacacgg tatcgggagc gatcgggccg
     1321 gttacatcat ggccttcttt ttttgccgcc tcgatcgccg gaatgatctg tcgaatctcc
     1381 tcatcgccga agagcccgtt ctcccctgca tggggattga ggcccgccac ggcgatcctc
     1441 ggatgagggt atccgaactt cttcatcgtc tccgcaccaa gccggatgac ctccagaacg
     1501 cgcttcttcg tcacagcgtc gcacgcgctt ctcaggcttt gatgggttga aacgtggatg
     1561 accgtcagct tgggactgga gaggagcatt gcgtaggaat cccctttggc gtagtgggcg
     1621 aagatttccg tgtggccggc aaaccggtgt cccgccagat gaagcgcttc cttgttcaag
     1681 ggagcggtga ttacgctgct gatctccttt ctccgggcaa actcgatcgc cgttcgcacc
     1741 gcttcaaagg cgacttcccc gcccaacgcg ctcacctttc cgataaggac gtcctcaagc
     1801 ttgaccggat ggggatccac gacattgatc atccccattt tgaactcttc gattgtttcg
     1861 atcacgttga aggtgaagtt cgctttcaat cgtttgttgt aatactccag cactgaaagg
     1921 ggaccgaaga ggagcgcttc cctttgatag gaaggttcct tcagcaacgc ttttatggtg
     1981 acttcgatgc cgatcccaca gggatcgccc aaggtaatgc caaagatttt cataattgcc
     2041 gttcctccaa acgtctcagg ctataggtca gtgcatcttc cttcccgaag cccccggcct
     2101 ttgtaacgat tttcatcccc tgatagggtc cgtcaagcac gagagtgatg ggaattccga
     2161 tatccacctc tccgatgatc ttcacttcat tgattcccat catctgcatc agcccgaatg
     2221 cggtctcacc gccactgaga aagaggtttt tcaccccgct tgctttgatg atttccaagg
     2281 acaccttcga gaggcaatcc tttatcatcg ttccgacaac agcgtccttc aagcccaacg
     2341 cttttcccac ctcgatcgat tgttggaata cggaccgatc cagggctgaa gagagggtga
     2401 ggatcagatc ctctccccct tggaggattt gtactccttg atcgatgatt ggagtaaagt
     2461 ctcccccttt gaggatcgaa aggacatcca cggcaaggat ggcgacccct cgactcttgg
     2521 cgtagtcaag ctgcgcacgg cttgctttgc tcaagcttgc gagaattgca agggccggct
     2581 gtttcggacg gtcgtattgg atcagcgctc ctgccaagcc ggcactgccg acatacaaaa
     2641 ttcgtttatc aagacctctc gcccatttga ccgtttcaaa gagatcggct tcatgctccg
     2701 cgtcgcagac gatgacctgt gcatccttga tgaggggaaa tgctttgctt cggatctcat
     2761 ccaccgagag gtgaatggtc gaatactcgg aagcaaagag ggtgataaga ttatcctccg
     2821 ttaccggttt gagcggatcg tgggcaaact ccgtatccaa gagctttacc gagttcacga
     2881 atacggttcc tccgacgcag gttcgtccca tctggctgaa cgccggggcg acgagaataa
     2941 gatcactgtt cattttttcg tcaatcgccc ggatctcttc cagaatattc cctcgaaggg
     3001 tcgaatcaat ttttttcagg acgatgtcgt atgagtcgat atcaaggtct ttgatgagag
     3061 ccttggtcat cctgtacgcc tcatcacctt ctctgtttcg ggactccgta tccaggacat
     3121 acgagtctac cgtccctttc ggcgaagaga agccgatgat cgtggagagc ccccgatgag
     3181 ccagttcaag agcactgtca ttggaacccg taaaatcgtc tgcgataatc aggtaggtat
     3241 tcatttgata cagtatactc cgtgacagag gtttacacaa actcatttca tcaagtacag
     3301 tggcggtata tgagtcttac gtacactcgg tttctcaccg attcgagctt cacccacgct
     3361 cagaccttcc gcgatccgct gtggaaggat atttcgattc caacctatat ggctgaggtg
     3421 gttcaaagcc cgagcttcat caaactgggg agaatcaaac agctcggcgt tgcgtttcac
     3481 ctgtaccccg gagcggtgca tacccggctc aaccacagtc
//
LOCUS       DAOPUZ010000078         3495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_27405_length_3495_cov_3.406395, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000078 DAOPUZ010000000
VERSION     DAOPUZ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27405_length_3495_cov_3.406395"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="PKN79_02120"
     CDS             <1..1306
                     /locus_tag="PKN79_02120"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNZ94588.1"
                     /translation="ENILLNREPLKHTLASELFGERLNTLNRESMEKIAQGAIDKLGV
                     PLDGKMLVSEMPVGHKQFTEIARELSKEATTEMEEGLRLLVLDEPTAVLSEQEAESLL
                     KAMRTLSSEGIAIIFISHRLQEIIDVCDTIMVMRDGRIIKTVPSEGVNVTDIAHWMVG
                     REVNSAKRNTRTFNYKDSKVVLSIRNLWVDMPGETVRNVNLDVYDGEILGIGGMAGQG
                     KLGIPNGVMGLFPAGGQIVFQGEELPLNQPRSLLNAGIAFVSEDRRGVGLLLDESLEW
                     NITFTAMQIQDKFLKRYLNGLVSWRDQKAIEEETMKYVKQLEIRCVSTRQNARELSGG
                     NQQKVCLAKAFAVTPKLLFVSEPTRGIDIGAKSLVLDALRRYNQESGTTIVMISSELE
                     ELRSVCDRIAIVNDGKVFGILPATSDSAKFGLMMVGQEVQEV"
     gene            1303..2364
                     /locus_tag="PKN79_02125"
     CDS             1303..2364
                     /locus_tag="PKN79_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94589.1"
                     /translation="MNAALMLTKIRQMVRSFGLPRIIIALFLFSLFILAPFFNVNVAT
                     SLSDTLNRFGMNAVLVLAMVPMIHSGCGLNFGLPLGIISGLLGATISLQVDVAGPASF
                     WLAILFATPFALILGWGYGQLLNRVKGGEMMIATYVGFSSVAFMCIMWLLLPFNNPKM
                     VWGFAGSGLRTTISTEGYYLHVLNDFLKIRIGNHFIFPTGMILFFTVLAFAMWAFLHT
                     KTGTAMTAVGSNPVFARASGIDVDRIRTLSVVLSTWLGAIGILVYQQSFGFIQLYMGP
                     FYMALPAVSAILIGGASVNKASILNVIIGTFLFQGILTMTPSVMNAMIQTDMSEVIRI
                     VASNGMILYALTRKTEAAR"
     gene            2361..3473
                     /locus_tag="PKN79_02130"
     CDS             2361..3473
                     /locus_tag="PKN79_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94590.1"
                     /translation="MRELSTKQKILRFMANNTVPLLFVVIIAFGVPLSGYSPTYLAGE
                     VISRISRNTFLIVSLLIPILAGMGLNFGMTLGAMAGQIGLIFTTDWGIVGIPGILLAM
                     IVSTPLSILLGWWCGKILNMAKGREMVTSYIIGFFMNGIYQLVVLYGMGPLIKIISPN
                     LLLPRGYGIRNTVNLIGIRKTLDNFLMIRIGGVPIPVGTFLVIALLSVFILWFKKTKL
                     GQDMRAVGQDMEVARAAGIRVEHTRIMSIIISTVLAGYGMIIYLHNIGTLNTYNSHMQ
                     IGMFSVAALLVGGATVASAKIRNVFLGVVLFHLMFIVSPMAGKRLIGQAQLGEYFRVF
                     VSYGVITTSLVLYEVRKKRERGMSDLTLAQDQEVTS"
BASE COUNT          762 a          924 c          995 g          814 t
ORIGIN      
        1 ggagaatatt ttgctcaatc gggagccgtt gaaacataca ttagcaagtg aactcttcgg
       61 cgagcggttg aacacgctta accgggagtc aatggagaag atcgctcaag gagcgatcga
      121 caagctgggt gttcctttgg acgggaagat gctcgtttcg gagatgcccg tcggccacaa
      181 acagtttacc gagatcgccc gggagctcag caaggaagcg acgaccgaaa tggaggaggg
      241 gcttcgactt ctggttctcg atgaaccgac cgcggtactc agcgaacagg aagcggaaag
      301 ccttctgaag gcgatgcgca ccctcagttc cgaagggatt gcgatcatct tcatcagcca
      361 ccgtctgcaa gagatcatcg atgtctgcga cacgatcatg gtgatgcgcg acggccggat
      421 tatcaagacg gttccttccg aaggggtcaa cgttacggat atcgcccact ggatggtagg
      481 tcgagaggtc aactctgcaa aacgaaatac ccgaaccttc aactataaag actcgaaggt
      541 tgtcctgagc atcaggaacc tctgggtcga catgcccgga gagacggttc gaaacgtcaa
      601 tcttgacgta tatgacggtg agattctcgg catcggagga atggcgggac aagggaagct
      661 cggcattccc aacggagtga tgggtctctt tcccgccggg gggcagatcg tttttcaagg
      721 agaggaactt cccctcaatc agccgcggtc gcttttgaac gccggcatcg cgttcgtcag
      781 cgaggatcgc cggggggtgg gattgctctt ggacgagtcc ctcgaatgga atatcacctt
      841 cacagcgatg cagattcaag acaagttcct caagcgctac ctcaacggac ttgtttcctg
      901 gcgcgatcag aaagcgatcg aagaggaaac gatgaagtac gtaaagcagc ttgagatccg
      961 atgcgtctcc acccggcaga acgcccgtga gctcagcggt ggaaaccagc agaaagtatg
     1021 tctggcgaaa gcgttcgccg taacgccgaa gcttctgttt gtcagcgagc cgactcgggg
     1081 aatcgatatc ggggcgaaga gtctcgtcct ggatgccctc aggcggtaca accaggaaag
     1141 cgggacgacg atcgtgatga tcagcagcga gcttgaagag ctgcgctccg tctgcgaccg
     1201 gatcgcgatc gtcaacgacg ggaaggtgtt cgggatcctc ccggcgacga gtgacagcgc
     1261 caagttcggt ttgatgatgg ttggtcagga ggtgcaagaa gtatgaatgc cgctctcatg
     1321 ctgacaaaaa tccggcagat ggttcgttcg tttggattgc caagaatcat catcgccctc
     1381 tttttgtttt cattgtttat tttggctccg ttcttcaatg taaacgtagc aacaagtctt
     1441 tccgatacct tgaaccgctt cggcatgaat gcggtgctgg tattggcgat ggttccgatg
     1501 attcatagcg gctgcggcct caacttcggt cttccgctgg ggatcatctc cggtctctta
     1561 ggtgcaacga tcagtcttca agtggatgtc gccggtcccg cctcgttttg gctggcgatc
     1621 ctttttgcca cgcccttcgc ccttattctt gggtgggggt acgggcaatt gctcaaccga
     1681 gtgaaaggcg gagagatgat gatcgcgacc tacgtcggat tctcctcggt cgcattcatg
     1741 tgcatcatgt ggcttctttt gccgttcaac aacccgaaga tggtctgggg ttttgccggc
     1801 tcgggattgc ggaccacgat ttcaaccgag gggtactacc tccacgtgct gaatgatttt
     1861 ctcaagattc gaatcggtaa tcacttcatc ttccccaccg gaatgatctt gttctttacc
     1921 gttttggcgt ttgccatgtg ggcgtttctg cacaccaaga ccggcacggc gatgaccgcc
     1981 gtcggatcca acccggtttt cgcccgggct tcaggaatcg atgtcgatcg gattcgaacc
     2041 ctcagcgtgg tgctttcaac gtggttgggg gcgatcggaa ttctggttta ccagcagagc
     2101 ttcgggttca tccagctcta catgggaccg ttctacatgg ctcttcctgc ggtatcggcg
     2161 attctcatcg gaggagcatc ggtgaacaag gcttcgattc tcaacgtcat catcggaaca
     2221 ttcctcttcc aagggattct cacgatgact cccagcgtga tgaacgcgat gattcagacc
     2281 gatatgagcg aagtgatcag gatcgtggcc tctaacggta tgatcctcta cgctctgacg
     2341 agaaaaacgg aggcggcccg atgagagaat tgagtacgaa acagaaaatc ctccgcttca
     2401 tggcaaacaa taccgtaccg cttcttttcg tggtcatcat cgccttcggg gttcccttga
     2461 gcggatattc accgacttat ctggccggag aggtgatcag tcgaatcagc cggaacacct
     2521 ttttgatcgt ttcacttctc atcccgatcc tcgccggaat ggggttgaac ttcgggatga
     2581 ccctcggagc gatggcgggt cagatcggcc tgatcttcac caccgactgg gggatcgtcg
     2641 gaattccggg aatccttttg gcgatgatcg tctccacccc gctttccatt ctgctcgggt
     2701 ggtggtgcgg aaagattctg aacatggcca aaggccggga gatggtgacc agctatatca
     2761 tcggtttctt catgaacggc atctatcagc tggtcgtcct ctatggaatg ggtccgttga
     2821 tcaagatcat ttcaccgaac ctgttgctgc cccgcgggta cggaatccgg aataccgtga
     2881 acctgatcgg catccgcaag acgctggaca actttttgat gatccggatc ggcggtgtgc
     2941 cgattccggt gggaaccttc ctcgtcattg cgctgctcag cgtcttcatc ctttggttca
     3001 agaaaacgaa gctgggacag gacatgcgag cggtcggcca ggatatggaa gtcgcccggg
     3061 cggcgggaat ccgggttgag cacacccgaa tcatgagcat catcatctcc acggttctcg
     3121 ccggatatgg aatgatcatc tacctgcaca acatcggaac gctcaacacc tacaacagcc
     3181 acatgcagat cgggatgttc agcgtcgccg ccctgctggt cggcggagcc accgtcgcca
     3241 gtgcgaagat tcgaaacgtc tttttgggcg tcgtgctctt ccacctgatg ttcatcgtca
     3301 gcccgatggc gggaaaacgc ctcatcggcc aggcccagct cggcgaatac ttccgggttt
     3361 tcgtttccta cggggtcatc accaccagtc tggttctcta tgaagtccgg aagaaacggg
     3421 agcgagggat gagcgatctt accttggccc aggaccagga ggtgacgtca tgacgaaccg
     3481 ccaacgaacc ctttt
//
LOCUS       DAOPUZ010000079         3478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_27584_length_3478_cov_3.242185, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000079 DAOPUZ010000000
VERSION     DAOPUZ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27584_length_3478_cov_3.242185"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1442
                     /locus_tag="PKN79_02135"
     CDS             141..1442
                     /locus_tag="PKN79_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ94591.1"
                     /translation="MLTRRVKFGFGIGDLGGNLFFTIIGFYLLYYFTDIVGLNPALSG
                     TAVMIGKVWDAITDPITGYYSDRTKSRFGRRRPYIFIGAIFSFFFMIQLFTPVSMKNE
                     MSLFLYYTLVYSLLNTAYTLVNIPYTALLPELTDDYHERTILTGYRMSFAVLGTFAGA
                     ALVMPILNATSWPVMGGVMGGIMLASAMITVFAIREPKEHSKPGEDGFLSTFAEVLKE
                     KVFLQALLPWTFFITGTMMIQGGLVYYFTYIYGNKDLFQLALVGLLSFSLLCIPFWVS
                     VSKKIGKRKVYMIGMSIVSIGVLLFTTLGAVFGPIFGIVVMSLAGIGLSTHYVMPHSI
                     LPDVVEYDAHTNKKGRREGVFSSIWTFSSKIGQAFALALNGWILAIFGYQGGSFLPQT
                     KPGIILIVGPLPILWYILGLLILRHYPIDQAYYNEMIKRGE"
     gene            1432..2685
                     /locus_tag="PKN79_02140"
     CDS             1432..2685
                     /locus_tag="PKN79_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94592.1"
                     /translation="MANKDRFPAPAYSPLARMIVPLFGKLYLRLALGVSAIVLLEGEI
                     LRNELRRFEERRSSLILAFRHTAVEDAPTLLVGVKHPHLRFLYGRDVVNWAGWPTKIL
                     FPALGFVAVENRGNNREGAQYLRKEVQNPRFPIALAPEGQVTYHAYTCASIEMGAANI
                     ASWALEGPREEVVILPIGIGYRYAKDNDRFLLDLITRWEKEANVTVDRSLPPNEQIRA
                     IGYETLKLVNTFWNLNLTLKGSFIKQRDGLCDALLRYSESLGGLEDSSGSIIDRLFRV
                     RYKAVEILNDTDRSLLSEEERRLHDEEVTKQKISDHASQVVDVLEYIDLNYLEGKHAV
                     QRSIEVMLSLLDVLNRLQGGMINSRFSPKGKKAFILGGTPIEVRKSFGHITGRKERLN
                     AINQALEEGLNNVSLTLEEIMFQST"
     gene            complement(2651..3331)
                     /locus_tag="PKN79_02145"
     CDS             complement(2651..3331)
                     /locus_tag="PKN79_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ94593.1"
                     /translation="MSFAKRFVNSVLRTYFRLFFKIDRSELVKVPLEGPLMMIVNHTS
                     VFEGPMLYAYLQPRAMHALAKKELWKNPVTRYLMNLWESIPVDRGNVDRQAMDAIFAV
                     LDKGEIFAIAPEGTRSRDGALQRGKSGIAFIAYKNRTPMVPIAVTGFENFRRNLLRLR
                     RTRITIKVGEVFRPIAQGGRLDAEGRQVLADEMMVRIASLMDERHQGYYKTFDPTFTL
                     TETLSLQG"
BASE COUNT          754 a          995 c          929 g          800 t
ORIGIN      
        1 tcacgatccc cctttcgttt gccctgatgg gataaggaaa gagttccctc tggagacaaa
       61 tgtccaaaca ccacccataa tcggcttgtt tacccccggt gtgtaagcta cactgggtgg
      121 taacgtgcag aggagggact atgcttacac ggcgagtaaa gtttggtttt ggcatcggtg
      181 acttgggggg caaccttttc ttcacgatta tcggtttcta tcttctctac tatttcaccg
      241 acatcgtcgg ccttaatccc gccttgagcg gaaccgcagt gatgatcgga aaagtgtggg
      301 atgcgatcac cgacccgatc accggctact actccgatcg aaccaagagc cgcttcggcc
      361 gaagaagacc gtatatcttc atcggggcga tcttctcctt tttcttcatg atccagctct
      421 tcaccccggt ctccatgaag aacgagatga gcctcttcct ctactacacc ttggtctact
      481 ccctgttgaa caccgcctac acgctggtaa acatccccta cacggcgctg ctgccggagc
      541 tcaccgacga ctatcacgag cgcaccatcc tcaccgggta tcggatgagc tttgccgtct
      601 tgggcacctt cgccggagcg gcgttggtga tgccgatcct gaacgccacc tcctggccgg
      661 tgatgggcgg ggtgatgggg ggaatcatgc tggcgtcggc gatgattacc gtctttgcga
      721 tccgggagcc gaaggagcat tccaagcccg gcgaggacgg atttctttcc accttcgccg
      781 aagtgctcaa ggaaaaggtg tttcttcaag cccttctgcc gtggacgttc ttcatcaccg
      841 gtacgatgat gatccaggga ggcctcgtct actacttcac ctatatctac ggcaacaagg
      901 atctgttcca gcttgccctc gtcggcctgc tctccttcag cctgctctgc atcccctttt
      961 gggtatccgt ctccaaaaaa atcggcaaga gaaaagtgta tatgatcggc atgtcgatcg
     1021 tgagcatcgg agtcctgctc ttcaccacgc ttggagccgt gttcggaccg atcttcggca
     1081 tcgtggtgat gagcctggcg gggatcggtc tttcgaccca ctacgtgatg ccccattcga
     1141 tcctgccgga tgtcgtcgag tatgatgccc ataccaataa aaaaggtcgc cgggaaggag
     1201 tcttttcaag tatctggacg ttcagcagca agatcggcca agcgttcgcc cttgccctca
     1261 acggctggat tttagcgatc ttcggctatc agggaggatc gttcctgcct caaacaaaac
     1321 ccggcatcat cctcattgtc ggtccgcttc cgattctttg gtacatcctc ggcctcctga
     1381 ttctaaggca ctacccgatc gaccaggcct actacaatga gatgatcaag cgtggcgaat
     1441 aaggatcggt ttccggcacc cgcatactcg ccgctcgccc ggatgatcgt accgctgttc
     1501 gggaagctgt atctcaggct cgccttgggg gtatcggcga tcgtgcttct cgagggggaa
     1561 attcttcgca atgagctgcg gcggttcgaa gaaagaagat catccctcat ccttgccttc
     1621 cgccatacag cggtcgagga cgctcccacg cttctcgtcg gcgtgaaaca ccctcatctc
     1681 cggttcctct atgggcggga tgtcgtcaac tgggccggtt ggccaaccaa gatcctcttt
     1741 cccgccctcg gcttcgtcgc cgtggagaac cgggggaaca accgggaagg cgcccaatat
     1801 ctccgcaaag aggtgcaaaa cccccgtttc ccgatcgccc tggctccgga aggtcaggta
     1861 acctatcacg catacacatg cgcctcgatc gagatgggag cggcgaacat cgcctcgtgg
     1921 gctcttgagg gtccgcggga ggaggtcgtc attcttccga tcggcatcgg ataccgatat
     1981 gcgaaagaca acgaccgttt cctccttgat ctcatcaccc gctgggagaa ggaagcgaac
     2041 gtcaccgtcg atcggagtct tccaccgaat gagcaaatcc gtgccatcgg ctatgagacc
     2101 ctgaagctgg tcaacacctt ctggaacctg aatctgacct tgaaaggctc cttcatcaag
     2161 cagcgggacg ggttgtgtga tgccctgctc cgttactcgg agtcgctggg cggtctggaa
     2221 gactcaagcg gctcgatcat cgaccggctc ttcagagttc ggtacaaggc ggtggaaatc
     2281 ctgaacgaca ccgaccggtc cttgttgagc gaggaagaac ggcgtcttca tgatgaagag
     2341 gtcacaaagc agaagatctc cgaccatgcc tcccaggtgg ttgatgtgct tgaatatatc
     2401 gatttgaact atttggaagg gaaacatgcc gttcagcgtt cgattgaagt gatgctctcc
     2461 cttcttgacg tgctgaatcg gctccaaggt ggaatgatca attcgcgctt cagccccaag
     2521 ggcaaaaagg cgttcatcct tggcggcacc ccgattgaag ttcgaaaaag cttcggacac
     2581 atcaccggga gaaaagagcg gctgaatgcg atcaatcagg cccttgagga gggcttgaac
     2641 aacgtgagcc tcacccttga agagataatg tttcagtcaa cgtaaaggtt ggatcgaagg
     2701 ttttgtaata tccctgatgg cgctcatcca tcaacgaggc gatgcggacc atcatctcat
     2761 cggcaagaac ctgccgtcct tcggcatcca ggcgtccccc ttgggcaatc ggccggaaca
     2821 cctcacccac cttgatcgtg atccttgtcc gccgcaatcg cagaaggttc cgtctgaagt
     2881 tttcaaagcc ggttacagcg atcggaacca tcggagtccg gttcttatag gcgatgaagg
     2941 cgattccgct ctttccccgc tggagggcgc cgtccctgct tcgggttcct tcgggagcga
     3001 tcgcgaagat ctcgcccttg tcgagcaccg caaagatcgc atccatcgct tggcggtcga
     3061 cattgccccg atcgaccgga atcgattccc aaaggttcat cagataccgg gtgacgggat
     3121 tcttccaaag ctccttcttg gcaagggcgt gcatcgctcg gggttgaagg tacgcataga
     3181 gcatcggccc ttcgaagacc gaggtatggt tgacgatcat catcaaggga ccttcgagag
     3241 gaaccttcac caattcactg cgatctatct tgaaaaagag tctgaaatag gttctcaaca
     3301 cgctgttgac gaaacgcttg gcaaaactca tggctcatcc tacaaaagag gatgaaaaga
     3361 aacaagcttt aatgagcgct tttcagcccc gccccggtaa ggggaatcag ggatgtttta
     3421 tcagccagca ttggatcttc cttcagggcg acccggtagc cggcgtagtt ggccgctc
//
LOCUS       DAOPUZ010000080         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_27869_length_3451_cov_3.466726, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000080 DAOPUZ010000000
VERSION     DAOPUZ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27869_length_3451_cov_3.466726"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PKN79_02150"
     CDS             <1..956
                     /locus_tag="PKN79_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HNZ94594.1"
                     /translation="GWAERGHVEQEFLAYLDENVAYPWSMIDKITPRPDAKIVEILKK
                     DGFKSGGLIVTKNNAHIAFFVNAEEAEYLVIEDTFKNGRPPLDKVGVYFTDRSTVNKV
                     ERMKVTTCLNPLHTALAIFGSILGFTLISEEMKDPQLRALVEGIGYTEGMPVVVDPGI
                     IDPRQFIDEVLNKRFPNPFMPDSPQRIATDTSQKLAIRFGETIKSYLEHPELDIKNLK
                     LIPLVYAGWLRYLMGIDDEGKPFTPSSDPRLEEAQEYVKGIKLGDKGPFKQLDGLLRD
                     KTIWGVDLIEVGLSTLVLSYFEKLIKGPGAVRQTLIDVVGP"
     gene            958..1902
                     /locus_tag="PKN79_02155"
     CDS             958..1902
                     /locus_tag="PKN79_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNZ94595.1"
                     /translation="MPTNVTKRLVVEIFYFLFGTALAGFAIALLITPAKIVGGGASGI
                     GTILYHLFGFDTGLVMIIVNIPIFFTGIIVFGKAYALRSFLGAIVLSVWVSIFGQLTS
                     YQGILPYADRVDTLLSAIFGGVILGAGIGVVMKSGANTGGTDILAQIISHFTPIPLGN
                     ALFLADAIVIGIGAVAFGIERALFAIITLYLSGQMVNYVVMNMGTKYAKTAYIVSEHY
                     QKIGERIITELQRGGTLIRGSGIYTSKERTMLMAVVHNQQITQLTKIVNEEDPKAFMI
                     VHETYQALGEGFVPMTSITSGPEHTSRSRRTPKRGKAD"
     gene            complement(2026..>3451)
                     /locus_tag="PKN79_02160"
     CDS             complement(2026..>3451)
                     /locus_tag="PKN79_02160"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ94596.1"
                     /translation="IMLGAGHPGFPLKGLDEDALDFIRKDLLNKCNLIEPRYIPVMEP
                     VLIDGRHILVLWVPGGDDRPYKCPVNVYTEKGAKRSEKAYYIRKGSNTIRANALEERE
                     LIGLARNIPFDDRINNHAKIADMRSSLLSEYLHNVGSGLYEGSLSRTVEDVATDMRLV
                     GGPSEMRRPINVGLMFFNERPDHFFPYSRIEVVDKPDPTGIGMKERVFTGPLDRQLRD
                     ALSYIGNYVIAEYVTKLPDRAEAVRVFNWPYRAVEEALSNAIYHRSYQIHEPITVTVT
                     PDRMEILSLPGPDRSITDDDLEKRVLVSSRYRNRRIGDFLKELKMVEGRNTGIPLIVK
                     EMKQNGSEPPIFKTDEDRSYFRVIFPIHRAFLRQSNHDVEEPKAEKSSKRRNREELRM
                     LVLEALEKTGGLSTSELARELGYKSLHDTLRAVVKELAASKEVAYLHPDKPNSRNQKI
                     FITKELSVSDHSHGTDERVLFTTC"
BASE COUNT          799 a          985 c          877 g          790 t
ORIGIN      
        1 agggctgggc ggagcgcggc catgtggagc aggagttcct cgcctacctg gatgagaacg
       61 tcgcctatcc ctggtcgatg atcgacaaga tcaccccccg cccggatgcg aaaatcgttg
      121 agatcctcaa gaaggacggc tttaaaagcg gcggcttgat cgtgaccaag aacaacgccc
      181 acatcgcatt ctttgtcaat gcggaagagg ccgaatatct ggtcatcgaa gataccttca
      241 agaacggcag gcctcctttg gacaaggtgg gcgtctactt caccgaccgt tcgacggtga
      301 acaaggttga acggatgaaa gttacgacct gtctgaaccc cctgcacacc gcccttgcaa
      361 tcttcggttc gatcctgggc ttcaccctca tcagcgagga gatgaaagat ccccagctca
      421 gggcgctggt ggaaggaatc ggatatacgg aagggatgcc ggtcgtggtt gatcccggga
      481 tcatcgatcc cagacagttc atcgacgaag tgttgaacaa gcgcttcccc aaccccttca
      541 tgccggacag cccccaacga atcgccaccg acacgagcca gaagctcgcg atccgcttcg
      601 gcgagacgat caagagctac cttgaacatc ccgaactgga catcaagaac ctcaaactga
      661 ttccgctggt ctatgccggc tggcttcgat acttgatggg cattgatgat gagggaaaac
      721 cgttcacccc cagctccgat ccccgtctgg aagaggctca ggagtatgtg aagggaatca
      781 agctcggcga caagggcccc ttcaagcaac ttgacggact gctgagagac aagacgatct
      841 ggggcgtcga tctgatcgaa gtcgggttaa gcaccttggt gctctcctac tttgaaaagc
      901 tcatcaaagg ccccggcgcc gtccggcaga ccttgatcga tgtcgtcgga ccgtaagatg
      961 ccaaccaacg ttacaaagcg gctcgtcgtg gaaatcttct atttcctctt cgggaccgcc
     1021 ttggccgggt tcgcaatcgc cttgctcatc accccggcga aaatcgtcgg tggcggtgca
     1081 agcggcatcg ggacgattct gtatcacctc ttcggatttg ataccgggct ggtgatgatc
     1141 atcgtcaaca tcccgatctt tttcacggga atcatcgttt ttggaaaagc gtatgcgctg
     1201 aggtcgttcc tcggcgcaat cgttttaagc gtatgggtga gcatcttcgg ccagcttact
     1261 tcctatcagg gaatcctccc ctacgcggac cgggtggaca cgctcctttc ggcgatcttc
     1321 ggcggagtca tcctgggggc ggggatcggc gtcgtgatga aaagcggggc gaataccggc
     1381 ggaacggaca tcctcgccca gatcatcagc cacttcaccc cgatcccgct cgggaacgcc
     1441 ctcttcctcg ccgacgcgat cgtcatcggg ataggagcgg tcgccttcgg gatcgagcgc
     1501 gccctctttg cgatcatcac cctctacctc tccggccaga tggtgaacta tgtggtgatg
     1561 aatatgggaa cgaagtatgc gaaaaccgcc tatatcgtca gcgaacacta ccaaaaaatc
     1621 ggcgaacgga tcatcaccga actccagcgc gggggaacgc tcatccgggg gtcgggaatc
     1681 tatacctcca aggagcggac gatgctgatg gcggtcgtgc ataatcagca gatcacccaa
     1741 ctgacgaaga tcgtcaatga agaggatccg aaagcgttca tgatcgttca tgagacctat
     1801 caggccctcg gcgaaggctt cgtcccgatg accagcatca cctccggccc ggaacacaca
     1861 agcaggagcc gacgaactcc aaagcgcgga aaagctgatt gaaaaaacct gccggtacaa
     1921 gaaaacgctc cttcaaactg tcccattaac actcccatcc atggtaatgg gacagtcaaa
     1981 gggcaagtcg gaacacaaac gcgagaatca gaagcgtgga aaatgtcaac aggtggtaaa
     2041 aagcaccctt tcatctgttc catggctatg atctgaaacc gatagttctt tcgttataaa
     2101 gatcttctgg tttctgctgt tcggcttgtc cggatggaga tatgcaacct ctttggatgc
     2161 cgccagctct ttgacaacgg ctcgaagcgt gtcatgcaaa ctcttatagc caagttcccg
     2221 agccagctcg cttgttgaaa gaccacccgt tttttccaac gcctcaagca caagcatcct
     2281 gagttcttcc ctgttcctcc gcttggagct tttctccgcc ttcggttctt ccacatcatg
     2341 gttgctttgc ctgagaaacg cccgatgaat cgggaatatc actcgaaaat agcttctgtc
     2401 ctcgtccgtc ttgaagatcg gcggctcgga accgttctgt ttcatctctt tcacgatcag
     2461 cggaataccg gtatttcttc cctcgaccat cttcaattct ttcaggaaat ccccgatgcg
     2521 ccgatttcga tatcggctgg agacaagcac cctcttttcc agatcatcat ccgtgatcga
     2581 tcggtcggga cccggaagac tcaggatttc catccgatcg ggagtaaccg ttacggtgat
     2641 cggctcatga atctgatatg agcgatggta gatggcattg ctcagagctt cctccaccgc
     2701 tctatacggc caattgaaca cccgaacagc ctccgcccga tcgggcagtt tcgtcacata
     2761 ttcggcgatg acgtaatttc cgatgtacga cagtgcatcc cgcagctgcc gatccaaggg
     2821 tcctgtaaaa acccgttctt tcattccaat ccctgtcggg tcgggcttgt ccactacttc
     2881 aatccggcta tacggaaaga aatgatccgg tctttcgttg aaaaacatca agcccacatt
     2941 gatgggtctt ctcatttccg aaggcccccc gacaagtctc atgtccgttg ccacgtcctc
     3001 aaccgtgcgg gaaagcgagc cttcatacaa tccgctgcca acgttatgaa gatactctga
     3061 aagcaggctt gaacgcatgt ccgcgatctt tgcatgattg ttgattctgt catcaaaagg
     3121 aatgtttctt gccagtccga tcagttcccg ctcttccaaa gcatttgccc ggatggtatt
     3181 ggaacccttg cggatgtaat atgctttttc actccttttg gctcctttct ccgtgtatac
     3241 gttgaccggg catttgtatg gtctgtcatc tcccccggga acccacaaaa caagaatgtg
     3301 ccgtccgtcg atcaacaccg gttccatgac cggtatgtat cgcggctcga tgaggttgca
     3361 tttgttgagg agatccttcc ggatgaagtc caaagcatct tcatcaagcc ccttgagggg
     3421 gaatccggga tgcccggcgc ccaacatgat g
//
LOCUS       DAOPUZ010000081         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_27873_length_3451_cov_2.617491, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000081 DAOPUZ010000000
VERSION     DAOPUZ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27873_length_3451_cov_2.617491"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /gene="tkt"
                     /locus_tag="PKN79_02165"
     CDS             complement(<1..1797)
                     /gene="tkt"
                     /locus_tag="PKN79_02165"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNZ94597.1"
                     /translation="MDVKQLANAIRILSMDGVQKANSGHPGAPMGMAEMAEVLFRRVM
                     KHNPKNPRWANRDRFVLSNGHASMLLYSLLHLSGYEVSLDDLKDFRQLHSKTAGHPEY
                     GDLPGIETTTGPLGQGFANAVGMALAEKSLAARFNREGHKVIDHYTYVFLGDGCLMEG
                     ISHEVASLAGVWKLGKLIVLYDDNGISIDGEVKDWFKDDTAKRFEAYGWHVIRDVDGH
                     DIDSVSKAIVGAQSIKDKPSLLMCRTTIGFGSPNKANTAGSHGSPLGSEEVALTRKNL
                     GWKHDEPFFIPEEIYKAWDQSARGAKDEEAWNAVFASYERAYPAEAAELKRRLANELP
                     EGWEKSFDDAIRKWQSEEVKVASRKASQMSLDILGNLLGELIGGSADLSPSNLTAWKG
                     VKDFDPKTGEGTYLRFGVREFGMMAILNGISLHGGFIPYGGTFLMFMEYARNAVRMAA
                     LMKIRSIGVFTHDSIGLGEDGPTHQPVEQMANLRMTPNMVTWRPCDLVETGVAWKMAI
                     KRSDGPTALLLSRQNLAPQKRTEKQLSLIEKGGYILHETGSSPRVILIGTGSEVELAV
                     KAAKVLEEEKISVRVVSMPRRRSSKRRTPSTRK"
     gene            complement(1911..2768)
                     /locus_tag="PKN79_02170"
     CDS             complement(1911..2768)
                     /locus_tag="PKN79_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94598.1"
                     /translation="MKRTIFVVLLIVGVSIGLVAQSGSVTAKLTGALGPGSVDLKAEG
                     AYQVKIPMLQGEGPLFEGNNLKVKAALGVSPIAAQVIVDAVLTPIALAEVNLGVAVGS
                     GWAFPLLKMNGLIKAPAEGAAMVDETLKGVYVKPKAGVALQFDTGAIFASKWASVVLR
                     AYQELNFTYYSNAEKDEPWEFELSGELYNSFNYKGEYIVGYKLPLEWVNLVGVQVEHH
                     VYDAFNRKHGGLVDASILVNSPFPIVDGLSLLMAIQFTNFKAQPDVRNRVVDRLRFKR
                     VALIATYSI"
     gene            complement(2841..>3451)
                     /locus_tag="PKN79_02175"
     CDS             complement(2841..>3451)
                     /locus_tag="PKN79_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94599.1"
                     /translation="PTPNAAEDEVVLSFIEEYKTTGLALDRNLSTEDGSLYQWILDTV
                     QKRFRTHGAIIIGRLIKEGELKRGVVPSSDFFSIFWPYRARTAGAERDMLATQIIDVL
                     SGDHPRSVRSLLSSGDWVSPFVVVTVTQDEIEAIKAFNERNVIAGDHLHMSFEERLPK
                     KPKIALDLQTYRLLLLQGVLSPKTGYTLERGELFEVLLDSMK"
BASE COUNT          738 a         1029 c          885 g          799 t
ORIGIN      
        1 cttccttgta cttggagtcc tgcgcttcga agacctccgg cggggcatcg agacaacccg
       61 gacggagatc ttctcttctt ccagcacctt ggccgccttg acggccaact ccacctcgct
      121 gccggttccg atgaggatga cccgcgggct gctgccggtt tcatgaagga tatacccgcc
      181 cttttcgatc agggaaagct gcttttccgt ccgcttctga ggggccagat tctgcctcga
      241 aagaagcaag gccgtcggtc cgtccgagcg cttgatcgcc atcttccagg cgacccccgt
      301 ttcgacgagg tcgcagggcc gccaggtgac catgttgggc gtcatcctga ggttcgccat
      361 ctgttccacc ggttggtggg tcggcccgtc ttccccgagg ccgattgaat cgtgggtgaa
      421 cacgccgatt gaacgaatct tcatcagcgc cgccatccgg accgcgttgc gggcgtactc
      481 catgaacatg aggaacgttc cgccataggg gatgaagccg ccatggaggg agattccgtt
      541 cagaatcgcc atcatgccga actcgcggac accaaagcga aggtacgttc cttccccggt
      601 cttcggatcg aagtccttga ctcccttcca agcggtaagg ttggaggggg aaagatccgc
      661 cgatccgccg atcagctcac cgaggagatt tcccaagata tccagactca tctggctcgc
      721 cttgcggctg gcgaccttca cctcttcgct ctgccacttc ctgatcgcat catcaaagct
      781 cttctcccaa ccctccggca gctcattggc caagcgccgc ttcaactccg ccgcttcagc
      841 gggatacgcc ctctcatagg aagcgaacac ggcgttccat gcttcttcat ctttcgctcc
      901 ccgggcgctc tgatcccatg ctttgtagat ttcttcgggg atgaagaacg gttcatcatg
      961 cttccaaccg aggttcttgc gggtcagagc tacttcttcg ctccccagcg gagagccatg
     1021 gctgcctgcc gtgttggctt tgttcggaga accgaagccg atcgtggtcc gacacatcaa
     1081 cagcgacggc ttatccttga tcgactgggc tccgacgatc gccttggaga cggagtcgat
     1141 atcatgaccg tcgacgtctc ggattacgtg ccatccatac gcctcgaagc gcttggcggt
     1201 atcgtctttg aaccagtcct tgacttcgcc gtcgatcgag attccattgt catcatagag
     1261 cacgatgagt tttccgagtt tccacactcc ggcaagggag gcgacctcat ggctgattcc
     1321 ctccatcaga catccgtcgc ccaaaaagac ataggtgtaa tgatcgatta ccttatgccc
     1381 ttccctgttg aatcgggcag ccagactctt ctcggcaagc gccattccga ccgcattggc
     1441 gaacccttgg ccgagcggcc cggtggtcgt ctcgattccg ggaagatcgc catactcggg
     1501 gtggccggcg gtcttgctgt gcaactgtct gaaatccttc aaatcatcaa gagaaacctc
     1561 ataaccactc aaatggagca acgaatagag cagcatcgaa gcatgtccgt tggaaaggac
     1621 gaagcggtcg cggttcgccc accgggggtt cttcggatta tgcttcatca ctcgtcggaa
     1681 gaggacctcc gccatctccg ccattcccat gggagctccc gggtgaccgg agttcgcctt
     1741 ttgtactcca tccatactta agattcgaat tgcattggca agctgtttta cgtccatcat
     1801 atcctccacg tatagttcaa caataaaaag ataccctact tactcaaggg tgtcaaaaga
     1861 caggcctgct tccggctcaa gaggcagact ccatccatag gttacataat tcagatcgaa
     1921 tacgtcgcga tcagggccac acgcttgaag cgcagccggt cgacgacccg atttcggaca
     1981 tcgggctgcg ccttgaagtt ggtgaattgg atcgccataa ggagtgaaag gccatcgacg
     2041 atcggaaacg gactgtttac cagaatcgaa gcgtcgacca gtcccccgtg ctttcgattg
     2101 aatgcgtcat agacgtggtg ctcaacttgg acgccaacca ggttcaccca ctcgagcggc
     2161 agcttgtagc cgacgatata ctccccctta tagttgaagg agttgtacag ctcaccgctg
     2221 agctcgaact cccacggttc atccttctcc gcgttgctgt agtaggtgaa gttcagctct
     2281 tggtacgccc gcagaaccac gctcgcccac ttcgaagcaa agattgctcc tgtatcgaac
     2341 tgaagggcga ctcctgcttt tggttttaca tacacccctt taagggtttc gtcgaccatc
     2401 gcagcccctt cagcgggggc ttttatgagt ccgttcatct ttaacagcgg gaaagcccat
     2461 ccgcttccga cggcgacgcc cagattgacc tcggcaaggg cgatcggagt caaaaccgca
     2521 tcgacgatga cttgggcggc gatcggcgag actccgagag cggccttcac tttcagattg
     2581 ttgccctcaa aaagaggccc ttctccctga agcatcggga tctttacctg ataggcgcct
     2641 tccgctttga gatccacgct tcccgggcca agcgctccgg taagctttgc cgttacactg
     2701 ccgctttggg cgacgagacc gatgctcacg ccaacgatga gcaagaccac gaatattgtt
     2761 cgcttcatag gaactcctac acattataaa attgtgttca tcataacaca cgataaaggc
     2821 aatgccatat actcaccata ctatttcatc gaatcgagca gcacttcaaa gagttccccc
     2881 ctttcaaggg tgtatcccgt cttcggacta agaacccctt ggagaagcag tagtcgatac
     2941 gtttgaagat caagggcgat tttgggcttt ttcggcagcc gttcctcaaa actcatatgt
     3001 agatgatcgc cggcgatgac attccgctca ttgaacgcct tgatcgcctc gatctcatct
     3061 tgcgtcaccg tgaccaccac gaagggggaa acccagtccc cgcttgaaag caagctcctc
     3121 acgcttctgg gatgatctcc ggagaggaca tcgatgatct gggttgcgag catatcccgc
     3181 tccgccccgg cggtccgggc gcggtacggc cagaagatcg agaagaaatc gcttgaaggc
     3241 acgactcccc ttttcagctc tccttccttg atcaggcgac cgatgataat cgctccatgg
     3301 gttctgaagc gtttttgaac ggtatccaag atccactgat acaagctgcc gtcttcggtg
     3361 gaaagattgc gatcgagagc gagaccggtt gtcttgtatt cttcgatgaa agaaagcacc
     3421 acttcgtcct ctgcggcatt cggagtggga a
//
LOCUS       DAOPUZ010000082         3437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_28020_length_3437_cov_3.290065, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000082 DAOPUZ010000000
VERSION     DAOPUZ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28020_length_3437_cov_3.290065"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PKN79_02180"
     CDS             complement(<1..617)
                     /locus_tag="PKN79_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNZ94600.1"
                     /translation="MKRVVIMLLLILIPALIIAAPSAALIMDKVIETQKSDTAAMDLK
                     LTLIEANGTERERRIQTLSQTVDDKTSIITVFLSPESVKNTRFLSVEGNDATEQWIYL
                     PALKRVRKISATEEGGSFMGSDFSYSDMASTTYDADEATHRLIEETATTYVVESIPKT
                     VKSYGKNIATVDKETFLPLKVEFYAPDEKTLLKTLTTLETGTVGTR"
     gene            complement(614..2992)
                     /locus_tag="PKN79_02185"
     CDS             complement(614..2992)
                     /locus_tag="PKN79_02185"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNZ94601.1"
                     /translation="MEKRRTLDWIIISLSIVLTALGLIGIGWLHVDSSTDAFIPTNAD
                     VVQINDRIKERFGSLDPVVVSIYAEDTILQPKYMEILKTITEEIEKLDGVSTANSIAN
                     VQHLEPSFDGVEAVSLYDGDFEALKNRIAEWPSFYEGTFLSAEHTVASVLIQTTLDYD
                     SKQLLHDLREVVAPYQGVVETSILGLPAVTEAISESLLSDLGILVPIVAVLIILILYY
                     FLRSFKATLLSLVPLIFSSSVALGIMAIANITFTMATMLVPVLLLIVGSAYTIHIFSH
                     FFQNQDSGTIEEVLRVVVKRNTYPIIAAAATTAFGFLAQLSSPLGPFRIFGLLSFIGV
                     VICAFSSLVLLPALIRVVYKQPNGKERKKQQNKDMRLMTAAMVVANHHGKVLLWVSMI
                     ILVIFLPLSYYFVESGTNMLDFFRPSSSLVKDSKRFNERMQGSFSMTVTIEPSEDVSI
                     LDPSTLKTIEGAIRRLEAEPVVGGVQSIIPFVKRMNQLLGPGEDEIVHTVDEEEVVFD
                     FFAFDDDFFPGFEEEPVEAQEVVASAGGQGEYEIPGDPARYGLADESELANLIAQYLL
                     LYSGNLDMFINDPLEPDVTALTLVLKSSDTNRLRTITTLIPTLFPEDWTIEVGGGEAV
                     ALALADLVTRSQIVSLFSSLIAVWFLVWFTLKSPKLATLALIPCLFALLAVFVSMLAF
                     SIKLDIVTSLLAALCIGIGVDYAIHLLSAIVRNDMPMDRIMQTTGKAILANAASVALG
                     FIGLLFSRFMPIANLGVLFCIAMATAAASALLLLPAIAVHYPKLMFTRRTKS"
     gene            3194..>3437
                     /locus_tag="PKN79_02190"
     CDS             3194..>3437
                     /locus_tag="PKN79_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNZ94602.1"
                     /translation="MNYTKIVATLGPSSDEYQIIKAILQAGCRVIRLNFSHGTHEDQQ
                     RRYDMARKAAVELGIEITIFMDLQGPKIRLGALAEPS"
BASE COUNT          854 a          853 c          971 g          759 t
ORIGIN      
        1 cgggttccga ccgtgccggt ctccaacgtc gtgagggttt tcagcaaggt tttttcatcc
       61 ggcgcataga actcgacctt caggggaagg aacgtctcct tgtccacggt cgcaatattc
      121 tttccatagc tcttgacggt cttgggaatc gactcgacga catacgttgt tgcagtttct
      181 tcgatcaggc ggtgggtcgc ctcatcggca tcatacgttg tcgatgccat gtcggagtag
      241 gagaaatcgc tgcccatgaa cgatcctccc tcttccgtgg cgctgatctt ccgtactcgc
      301 ttgagcgccg gaagatagat ccactgttcg gtagcgtcgt ttccttcaac ggagagaaag
      361 cgggtgtttt taacgctttc gggagagagg aagacggtga tgatcgaggt cttgtcatcg
      421 accgtctggc tgagggtctg gatccgccgc tcccgttcgg tgccgttggc ctcgatgaga
      481 gtcagcttca gatccatcgc cgcggtgtcg gacttctggg tttcaatgac cttgtccatg
      541 atcagggcgg cggacggagc agcgatgatc aaggcgggaa taagaataag aagaagcatg
      601 ataaccacac gtttcatgat ttggtcctcc ttgtgaacat gagcttggga taatgaacgg
      661 ctatcgcagg aagcaacagc agcgcgctgg ctgcagctgt tgccattgcg atgcaaaaca
      721 gcactcctaa atttgcaatc ggcatgaagc ggctgaacaa caggccgatg aaaccgaggg
      781 cgacggatgc ggcattggca agaatcgcct tgccggtcgt ctgcatgatc cgatccatcg
      841 gcatatcgtt gcggacgatc gcactgagga ggtggatcgc atagtcgact ccgattccga
      901 tgcacagcgc cgcaagcaag ctggtgacga tatccagctt gatcgagaag gcaagcatcg
      961 aaacgaagac cgccaagagg gcgaagaggc aggggatcag ggcaagggta gcgagtttcg
     1021 gagatttgag ggtgaaccac accaagaacc acacggcgat cagggaactg aacagactga
     1081 cgatctggct tcgggtgacc aaatccgcaa gagcaagggc caccgcttca ccgccaccga
     1141 cctcaattgt ccaatcttcg gggaagagag ttggaatcag agtggtgatg gttcgaagcc
     1201 ggttggtgtc cgaactcttc aggacaaggg tcaatgcggt gacatcgggt tccaagggat
     1261 cattgatgaa catatcgagg ttgcccgagt agagcagcag gtattgggcg atgaggttcg
     1321 ccagctccga ttcatcggca aggccgtaac gcgccggatc gccgggaatc tcatattcac
     1381 cttgccctcc ggcgcttgca accacctctt gtgcttcgac cggctcttcc tcgaaacccg
     1441 gaaagaagtc atcgtcaaag gcgaagaagt caaagaccac ctcttcttca tcaacggtgt
     1501 gaacgatttc atcctcaccg ggaccgagaa gctggttcat ccgcttcacg aacggaatga
     1561 tcgattgcac tcctcccacc accggttcag cttcaagacg ccggatcgcc ccctcgatcg
     1621 tcttgagggt ggaaggatcg agaatcgaga cgtcctcgct cggctcgatc gtcacggtca
     1681 tcgagaagct cccctgcatt cgttcattga agcgcttgga gtctttgacc agggatgaag
     1741 aggggcggaa gaagtcgagc atgttcgttc cgctctccac aaagtagtag gagagcggaa
     1801 gaaagatcac cagaatgatc atggagaccc acagaagcac tttgccgtga tggtttgcca
     1861 ccaccatcgc tgcagtcatc aaccgcatat ccttgttttg ttgcttcttc cgctcttttc
     1921 cgttcggttg tttgtagaca acccgaataa gagcaggcag aaggacaagg gaactgaagg
     1981 cgcagatgac gacaccgatg aagctgagaa gtccgaagat tctaaagggt cccaagggag
     2041 aggagagctg ggccaaaaag ccgaaggcgg ttgtagccgc agcggcgatg atcgggtagg
     2101 tgtttcgctt cacgacgact cggagcacct cttcgatcgt tccggaatcc tgattttgga
     2161 aaaagtgact gaagatgtgg attgtatagg cgctgcccac gatgaggagc aataccggaa
     2221 cgagcatcgt cgccatcgtg aaggtgatgt tggctattgc catgattccg agcgcgacgg
     2281 aggaggagaa gatgaggggg acgagcgaaa gcagcgtcgc tttgaaggag cgaaggaagt
     2341 agtacaggat gaggatgatg aggacggcca cgatcgggac taaaatcccc aagtcactga
     2401 gtaaactctc actaatcgcc tcagtgactg cggggagacc taagatcgag gtttcaacaa
     2461 ccccttggta aggagcgacg acttccctga ggtcatgaag aagctgtttt gaatcgtagt
     2521 ccaaggttgt ttgaataagg acggaagcaa cagtatgttc ggccgacagg aatgtcccct
     2581 cgtaaaagga gggccattcg gcgatccgat tcttcaacgc ctcaaagtcg ccgtcgtaca
     2641 gcgataccgc ctcaacccca tcaaaggatg gttccaggtg ttgtacattt gcaatcgagt
     2701 tggcggtgga gactccgtcc aatttctcga tctcctcggt gatcgtcttc aagatctcca
     2761 tgtacttcgg ttgaaggatc gtatcctctg cgtagatgct cacgaccaca ggatccaacg
     2821 agccaaagcg ttctttaatt cgatcattga tctgcacgac atccgcattt gtcggaatga
     2881 aggcatcggt agaactgtcc acatgaagcc agcctatgcc gataagaccc aaggccgtca
     2941 ggacaattga aagtgaaata atgatccaat cgagggtgcg tcgtttttcc atgatcatag
     3001 actccctctt tagggcaaat caagcaatcg ccttcagacg gtattttcat cacccgtcgg
     3061 ggtgatttgg atatcacctc atggaaatcg aggaaaaaag gcttgaagag gtttacatgt
     3121 ccgatcttgg gtaatgatcg gtatcgctgt ctttccgctt tacaaaacgg attcaatcac
     3181 agatgattga cctatgaact ataccaaaat tgtagccaca ttagggccca gtagtgatga
     3241 gtatcagatc atcaaagcga tccttcaagc gggctgtcgg gtcattcgat tgaatttcag
     3301 ccacggcacg catgaagacc agcaaagaag gtacgacatg gcccgaaaag cggccgtcga
     3361 attgggaatc gagattacga tcttcatgga cctgcagggt ccgaagattc gcctgggagc
     3421 gttggctgag ccttcct
//
LOCUS       DAOPUZ010000083         3427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_28130_length_3427_cov_3.552788, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000083 DAOPUZ010000000
VERSION     DAOPUZ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28130_length_3427_cov_3.552788"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PKN79_02195"
     CDS             complement(<1..495)
                     /locus_tag="PKN79_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNZ94603.1"
                     /translation="MIYTRIENKQGLTMEAAKAAFMEGLAKLGKRERVLLIPPDITRI
                     HGLGGMLAVWATEFYGSAVKQILPALGTHVPMTPKEIEVMYPGVDPSLFTVHDWRNDI
                     VTIGTVPKEFIEEVSEGKLSYEWKAQVNKALVHGGFDLILSLGQVVPHEVIGMANYNK
                     NVFVG"
     gene            complement(579..1517)
                     /locus_tag="PKN79_02200"
     CDS             complement(579..1517)
                     /locus_tag="PKN79_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HNZ94604.1"
                     /translation="MSTYRFPPLTDFLIDQIAFAMEDQNMLSSLDLKEGEVITDDERS
                     FLDEEEREDPHRFIELPEWTSADGFRLMEEYAEGVRNDAIRKRLFFALNRGKGVFRAF
                     KDVLAEEPALERKWYLFKEQELRKQVILWYQREAGAAELEALPPEDEELFEEILLEDF
                     TFEACDLFDTEEIRQFIHTLLLEIGTFSGAGLLIERALQNTGRKLTHLARTATGELAG
                     LIVYTLANDSTAEVLLYGINVRYRGLGLFRLLFDGFSRQLGRLRVNRIIFGVPEQNPK
                     LKELFIRHGATPIATAYELTTHQWNTNHANSEQAFL"
     gene            1630..2925
                     /gene="eno"
                     /locus_tag="PKN79_02205"
     CDS             1630..2925
                     /gene="eno"
                     /locus_tag="PKN79_02205"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNZ94605.1"
                     /translation="MSIIEFIEAREILDSRGNPTVEVDVILEDGSMGRAAVPSGASTG
                     VHEAVELRDGDKSRYLGKGVLKAVDNVNTIIAPELEGLDALDQVGIDRAMIALDGTSN
                     KSKLGANAILGVSMAVARAAADHLSLPLFKYLGAFHANVLPVPMANILNGGAHSDNKV
                     DFQEFMVMPIGAPSFREGLRMTAEVFHALKAVLSSRGLNTNVGDEGGFAPDLQSNEEA
                     LEVIMEAIKKAGYTAGRDGDFMIALDPAVSELYDSKTKTYTLKWTTGEKLTNAQMVDM
                     WEDWCNRYPIISIEDGMDEDDWEGWKLLTDRIGDRVQLVGDDLFVTNSERLKMGLEKG
                     VANSILIKVNQIGTLTETFEAIDLAKRNGYTAVVSHRSGETEDNFIADLVVALETGQI
                     KTGSMSRSDRLAKYNQLLRIEDYLDETAQYAGRGAFRVL"
     gene            3052..>3427
                     /locus_tag="PKN79_02210"
     CDS             3052..>3427
                     /locus_tag="PKN79_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNZ94606.1"
                     /translation="MLDVSTLWSLQGQLLLLIAAGALVRRLKLIDEEGQAALTNVVLY
                     VTLPCSILLSFMMEVDHSLLSHLLVVFLLSAGVQVFSFTLSKLHYRNVEESKRNVLRY
                     GLLVSNAGFMGLPIAGLLFGQLG"
BASE COUNT          755 a          946 c          954 g          772 t
ORIGIN      
        1 gccgacgaag acgttcttgt tgtagttggc cataccgatc acctcgtgcg gcactacctg
       61 gcccaacgag agaatcagat caaagcctcc atggacgagc gccttgttca cttgggcttt
      121 ccattcatag gagagcttgc cctcactgac ctcttcgatg aactccttcg gtaccgttcc
      181 aatggtgacg atgtcgtttc tccagtcatg gaccgtgaag agggacggat cgacaccggg
      241 atacatcacc tcaatctctt taggggtcat cggtacatgg gttcccaagg cgggaagaat
      301 ctgttttacg gcgctgccgt agaactcggt cgcccatacg gcgagcatcc cgcccaagcc
      361 gtggatccgg gtgatgtccg gggggatgag caagacccgt tcccgcttgc ccagcttggc
      421 aagcccttcc atgaacgcgg ctttggccgc ctccatggtc aacccctgtt tattctctat
      481 ccgtgtgtat atcatcgata cctccagtat atgcgataca gcgcccacat cgtatcatga
      541 aggcgataat tccaacaagg tgaaagaatc tctcgtctct agagaaacgc ctgttcactg
      601 tttgcgtggt ttgtgttcca ctgatgagta gtcagttcat aggccgttgc aatcggggtc
      661 gctccgtggc ggatgaacag ctctttcagc ttcgggttct gctccgggac gccgaagatg
      721 atccggttga cccgaaggcg gcccaactgg cgactgaagc cgtcaaacag gaggcggaac
      781 aagccgagcc cccggtagcg cacattgatg ccatacagca gaacctcagc ggttgaatcg
      841 tttgcaaggg tatagacaat gagaccggca agttcgccgg tggcggtgcg ggcgaggtgg
      901 gtgagctttc gccccgtatt ctgaagcgcc cgctcaatga gaagacccgc tccgctaaag
      961 gttccgatct ccagcagaag cgtatgaatg aactgtcgga tctcttccgt atcgaagagg
     1021 tcgcacgcct cgaaagtgaa gtcctcgaga agaatctcct caaacagttc ttcgtcttcc
     1081 gggggaaggg cctccagctc ggcggcacct gcttcccgtt ggtaccagag aatgacttgc
     1141 ttccgaagct cctgctcttt gaagaggtac cacttccgct ccaaggccgg ttcttcggca
     1201 aggacgtcct tgaacgcccg gaacacccct ttccctcggt tgagggcaaa aaagagccgc
     1261 tttcggattg catcgttgcg aaccccttcg gcgtactctt ccatcaggcg gaagccgtcg
     1321 gcgctggtcc actcgggcag ctcgatgaag cgatggggat cttcccgctc ctcttcatca
     1381 agaaaagagc gctcatcatc ggtgatgact tccccctctt tcaggtcgag ggagctcagc
     1441 atattctgat cttccatcgc aaaggcgatc tgatcgatca ggaagtcggt aaggggtgga
     1501 aatcggtagg tactcatggt aaaagtatac tcttacgctt gttttccgac aaggttggca
     1561 ttcttgacac atacgtcaaa agaacggaaa ctaatagggt aaacgaatta caaacagagg
     1621 agaagtttga tgagtattat tgaatttatc gaagccagag agattctgga ctcccgtgga
     1681 aaccccactg ttgaagtaga tgtcatcctt gaggatggat ccatgggtcg ggctgcggtg
     1741 ccctccggag catcaactgg tgtccacgaa gctgtcgagc ttcgcgacgg cgacaagagc
     1801 cgctacctcg gtaagggtgt tttgaaagct gttgacaatg tcaataccat cattgctccc
     1861 gagctggaag gccttgatgc cctggatcag gttggaatcg atcgggcgat gatcgctctc
     1921 gatggaacct cgaacaaatc aaagctgggt gcaaacgcta ttttgggcgt ttcgatggca
     1981 gtcgcccgtg cggcagccga ccatttgagc cttcctttgt tcaaatatct cggcgcgttc
     2041 cacgccaacg tgctgcctgt tccaatggcg aacatcctca atggcggagc gcacagcgac
     2101 aacaaggtcg atttccagga gtttatggtc atgccgatcg gcgctccctc cttccgcgaa
     2161 ggcctcagaa tgaccgccga agtgttccac gccctgaagg cggttctctc ttccagaggg
     2221 ttgaacacca acgtcggcga cgagggcgga ttcgcccccg atctccaatc caatgaagag
     2281 gctcttgaag tgatcatgga agcgatcaaa aaagccggat ataccgccgg ccgcgacgga
     2341 gacttcatga tcgccctcga tccggcagtc agtgaactct acgactcgaa gaccaagacc
     2401 tataccctga agtggaccac cggcgagaag ctcaccaatg cccagatggt cgatatgtgg
     2461 gaagactggt gcaaccgcta tccgatcatc tccatcgagg acggaatgga cgaggatgac
     2521 tgggaagggt ggaagcttct gaccgaccgc atcggcgacc gtgtccagct tgtcggcgac
     2581 gacctctttg ttaccaacag cgagcggctc aagatgggtc ttgagaaggg tgtcgccaac
     2641 tcgattttga tcaaggtgaa ccagatcggt acgcttaccg agacgttcga agcgattgat
     2701 cttgccaagc ggaacggtta taccgcagtc gtctctcacc gctcgggtga aacggaagac
     2761 aacttcatcg ccgacttggt cgtcgccctc gagaccgggc agatcaagac cggttcgatg
     2821 agccgcagcg accgccttgc caagtacaac cagctccttc gcatcgagga ttaccttgac
     2881 gaaaccgctc agtatgccgg tcggggcgca ttccgcgtgc tgtaagacgg caatactatc
     2941 agacattcag ggccaccgcg aggtggtcct ttctatgaag agtggttgaa tcggtgctat
     3001 gctttacgca tacaggcagg aatgggaaag cgatgagacg gtggtgagaa gatgttggat
     3061 gtatcgacgc tctggagtct gcaaggtcag ctgcttcttc tgattgccgc aggagccctt
     3121 gtccgaaggt tgaagctcat tgacgaggag ggacaagccg cgttgaccaa cgtggttctc
     3181 tacgtgacgc tgccgtgttc catcctcctc tccttcatga tggaggtcga tcattccctc
     3241 ctttcccatc tgcttgtcgt gttcctgctc tcagccgggg tgcaggtctt cagcttcacc
     3301 ctctccaagc tgcactatcg aaatgttgag gagtccaagc ggaacgtgct ccgctacgga
     3361 ctgctcgttt ccaacgccgg cttcatgggg ctgccgatcg ccggattgct cttcgggcag
     3421 ctcgggt
//
LOCUS       DAOPUZ010000084         3426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_28146_length_3426_cov_2.436369, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000084 DAOPUZ010000000
VERSION     DAOPUZ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28146_length_3426_cov_2.436369"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PKN79_02215"
     CDS             <1..722
                     /locus_tag="PKN79_02215"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNZ94607.1"
                     /translation="GSIRTQKSRKQRSWNDRRNARRHYRFSSLAQWVQNHWLPDVEVT
                     FFMFTILSLALCVMELSKGREDAYAASILRLANGILILFYPGLLITFIQRITVFQAPG
                     ALLVLFFLIIFGNDIFAYVFGVWLGKNNRGWASVSPNKSIAGLVGGILSAVVLGGLFV
                     FFVPALSAALSLPKAIILSVLTAVVANIGDLIESAFKRSAKMKDSGTLIPGRGGLLDS
                     IDSMLVAAPVFYFLLAGFGVQ"
     gene            719..1822
                     /gene="dxr"
                     /locus_tag="PKN79_02220"
     CDS             719..1822
                     /gene="dxr"
                     /locus_tag="PKN79_02220"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNZ94608.1"
                     /translation="MKRIAILGATGSIGTTTLSVLRDRTDLPLKVVSLSAHNNRERLL
                     ALANEFSSEALCFTGSKEEGFFHGYEGLKAMLEEYPVNIVLNGIAGFDGLKASVAVLE
                     AGYDLALANKESVVCGGELLFELARKHNAKIIPVDSEHSAIYELLKGREREDVSSLIL
                     TASGGPFRNRDLSTFDSISVGDALAHPTWQMGKKISIDSATMANKALEVIEASYLFGF
                     PDDAIEVVIHPQSIVHSMIRTRDGAVYAQLGNPDMSLPIINALTSMSSNLVTPLDFNH
                     LMLTFEAIDEKRFPLLRCGYDALSGQGGYPLAFNAANEVAVEAFLSERITYPRLIHTV
                     QRTLDHDWTFTPKSVEEIIALDSEVRSKAKELM"
     gene            1819..3198
                     /gene="rseP"
                     /locus_tag="PKN79_02225"
     CDS             1819..3198
                     /gene="rseP"
                     /locus_tag="PKN79_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNZ94609.1"
                     /translation="MSYFLAQVIKYLIGIVGITVVVLIHELGHFVAARICGIDVEVFS
                     FGFGPRLIGKHIGDLDLRLSLFPVGGYCRLKGSDDVRQSLRNEQSLFLEEGSIFAASP
                     IRRIITYLAGPVASFLLALLIYTILSLMPYEVLSTEAIVTTVNDYPELFGPEATSPAY
                     DAGIRSGDRVLALNGETIDDWEALETILPVSPEHLFMVERNGQSFHYWVEGEKQADGT
                     YRYGLAVLQEAIVGNVRSLSTEKEAGLKKGDRIIASQGTVVHNHLDLLSTLRGELSRV
                     EFTVLRDGEEVRLSFHPRRGDDGKPIFDFSLLAATKHVKPTSKALTSGVGQTSKILHQ
                     TLDSIRSLFTGTQKDLRSNVTGVTRSALLIGDIAALGFEQETKQGLHALLYLLGVVSI
                     SLAVVNLLPLPAFDGIQIMVAFLALITGHTIKMRTYYVLQVAGVLFIIVVFIALAGSD
                     LRYLLSIRR"
     gene            complement(3188..>3426)
                     /locus_tag="PKN79_02230"
     CDS             complement(3188..>3426)
                     /locus_tag="PKN79_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ94610.1"
                     /translation="SIEQDADVVILLHRDTTKYRTDDEEQRERNNVQETKVIMAKNRN
                     GETGSFSIAFVRNIVRFEDLVTTRPYTAAANPNA"
BASE COUNT          698 a          966 c          909 g          853 t
ORIGIN      
        1 gggggagcat acgaactcag aaatctcgca aacagcgctc atggaacgac cggcggaatg
       61 cccgtcgtca ttaccgcttt tcttcccttg cccagtgggt tcagaaccac tggcttcccg
      121 atgttgaagt gacgtttttc atgttcacca tcctatcctt ggcgctctgc gtcatggagc
      181 tgtccaaagg cagggaagat gcgtatgccg cttcgatcct ccggctggcg aacgggatat
      241 tgattctctt ctacccgggt ctgctcatca ccttcatcca gcggatcacg gtctttcagg
      301 ccccgggagc gttgttggtg ctcttcttcc tgatcatctt cggcaatgat atctttgcgt
      361 atgttttcgg ggtatggttg ggtaaaaaca atcgcggttg ggcgagcgta agtcccaaca
      421 aatcgattgc aggccttgtg ggtggaattc tttcggcggt ggttctcgga ggactctttg
      481 tgttctttgt accggcgctc tccgctgcgc tctccctccc caaggcgatc attctcagtg
      541 tgctcactgc ggttgtcgcc aatatcggtg atctgatcga atcggcgttt aaacgatccg
      601 caaagatgaa ggacagcggc accttgattc cgggccgagg aggcttgctc gattcaatcg
      661 attcgatgct cgttgctgcc cctgtctttt attttctcct cgccggcttt ggcgtgcaat
      721 gaaacggata gcgatcctcg gagccaccgg ctcgatcgga acgaccaccc tttcggtgtt
      781 gcgcgaccga accgatcttc ccttaaaggt ggtctcgctc tccgcccaca acaacaggga
      841 acgcctcctc gctctggcca atgaattcag ctctgaagcg ctctgcttta ccggcagcaa
      901 ggaagagggg tttttccacg gctatgaagg actgaaggcg atgcttgagg aatatccggt
      961 gaatatcgtc ctcaacggga tcgccggctt cgacggcttg aaagcttccg tggccgttct
     1021 cgaagcaggc tatgatcttg ccctcgcaaa caaggaatcg gtggtgtgcg gcggcgagct
     1081 tctctttgag ctggcaagga agcacaacgc aaagatcatt ccggtggaca gcgaacattc
     1141 ggcaatctat gagctcttga aaggaagaga acgagaggat gtctcctccc tgattcttac
     1201 cgccagcggc ggaccgttta gaaaccgcga tctttccact tttgattcga tcagcgttgg
     1261 cgatgcgttg gcccacccca cttggcagat ggggaaaaag atctcgatcg attcagccac
     1321 catggcgaac aaagcgttgg aagtgattga agcttcctat ctcttcggct tcccagatga
     1381 tgcgatcgaa gtcgtcatcc acccgcaatc gatcgtccat tcgatgattc gtacccggga
     1441 tggagcggtc tatgcccaat tggggaatcc cgacatgagc cttccgatca tcaatgcact
     1501 cacgtcgatg agctccaatc tggtaactcc gctggatttc aaccacctga tgctgacgtt
     1561 tgaagcaatc gatgagaagc gctttcccct ccttcgctgc ggctatgatg ccctatccgg
     1621 ccaaggggga tatcccttgg cgttcaatgc ggcaaatgaa gtcgccgtcg aggcgttttt
     1681 atcggaacga atcacctatc cccggctgat tcatacagtc caacgaacct tggaccacga
     1741 ttggacgttc actccaaaat ccgtggagga gatcatcgcc ctcgacagcg aggtccgctc
     1801 aaaagccaag gagctcatgt gagctacttt ctggctcagg tgatcaaata cctcatcggc
     1861 atcgttggaa tcaccgttgt cgtgttgatt catgagctcg ggcactttgt tgccgcccga
     1921 atctgcggca tcgacgttga agtcttctcg tttggcttcg gcccgcgtct catcggcaaa
     1981 catatcggcg atcttgattt gaggctctcg ctctttcccg tcggtggata ctgtcggctc
     2041 aaaggaagcg atgatgtccg gcaatcgctg aggaatgaac aatctctttt tctggaagag
     2101 ggttcgattt tcgccgcatc accaatcagg cggatcatca cctacctcgc aggtccggtc
     2161 gcttcgtttc ttcttgccct gcttatctac acaatcctct cgctcatgcc gtacgaggtc
     2221 ctttccaccg aggcgatcgt tacgacggtg aacgactatc ccgagctctt cggcccggaa
     2281 gcgacgagtc ccgcctatga tgcgggaatt cggagcggtg accgcgtact cgccctcaac
     2341 ggggaaacaa ttgatgactg ggaagcattg gagacgatcc tgcccgtttc acctgagcac
     2401 ttgttcatgg tcgaacggaa cgggcaatcc ttccattatt gggtggaagg ggaaaagcag
     2461 gcggatggaa cgtatcggta cgggcttgcc gtccttcagg aagcgatcgt cggcaacgtt
     2521 cgttccctct cgacggaaaa ggaagccgga ctgaaaaagg gagacagaat catcgcctct
     2581 cagggaacgg tcgttcataa tcacctggat ctgctttcaa ccctccgggg ggaactgagc
     2641 cgggttgagt tcacggttct gcgggacggt gaagaggtgc ggctctcctt ccatccccgc
     2701 cgcggcgacg atggaaagcc gatcttcgat ttctccctgt tggctgcgac aaaacatgtc
     2761 aaacccacca gcaaggcact gacctccggc gtcgggcaaa cctccaaaat tctccatcaa
     2821 acccttgaca gcatccgctc cctcttcacg ggaactcaga aagacctgcg ctccaatgtt
     2881 accggagtta cccgctcggc gctcctgatc ggtgatatcg cagcgctcgg cttcgagcag
     2941 gaaacgaaac aaggccttca cgccctgctc tatctgctcg gggtggtttc gatcagtctt
     3001 gcggtggtga acctgcttcc cctgccggct ttcgacggaa ttcagatcat ggtcgcattt
     3061 cttgcactca ttaccggtca cacgatcaag atgcggacct attacgtgct tcaggtggcg
     3121 ggggttctct tcatcattgt ggtgttcatc gcccttgcgg gctccgatct tcgatacctt
     3181 ctttcgatca ggcgttagga tttgccgctg cggtatacgg cctggtcgta accaagtctt
     3241 caaaacgaac gatattgcgg acgaatgcga tggaaaagct tcctgtctct ccgtttcggt
     3301 tcttcgccat gatcaccttc gtttcctgga cgttgtttct ttcccgctgt tcttcatcgt
     3361 cggtccgata tttcgtggta tcccgatgaa gaaggatcac gacgtcggca tcctgctcga
     3421 tcgagc
//
LOCUS       DAOPUZ010000085         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_28152_length_3425_cov_3.251929, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000085 DAOPUZ010000000
VERSION     DAOPUZ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28152_length_3425_cov_3.251929"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1185
                     /locus_tag="PKN79_02235"
     CDS             115..1185
                     /locus_tag="PKN79_02235"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HNZ94611.1"
                     /translation="MKPLMTVTLAGGAQTEVLLAEDIKDLSAHLGDYGRSVLWVFDSN
                     TNKLFRQLPPARIILDSGEQSKNLTSLERIISLALDEGLGRDGRIIGFGGGVVGDISS
                     LAASLYMRGCRLTLVPTTLLAMVDASIGGKTAVDFQGMKNMIGTFYPANEVLISIDVL
                     KSLSEREYKSGLAEVIKHALLSEDQELFKLLLSRRSEILNREEETLMKLVELSLLVKK
                     SYIERDPTETKGIRKALNLGHTFGHALESSTRFAAFSHGAAVAWGTVRALEAGVALGI
                     TDKKYASSASKLFGSFGYDLDHRIGRGEWIEYKNHLSKDKKKLGGEVQFVLLGGNGKP
                     ELHPLPWNLIQALVMEKAIGRG"
     gene            complement(1169..>3425)
                     /locus_tag="PKN79_02240"
     CDS             complement(1169..>3425)
                     /locus_tag="PKN79_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNZ94612.1"
                     /translation="AYKMRFEDTTDYTTRIKVMTDGILLMELKNDPMLMQYSVILVDE
                     AHERSLNIDFILGLLKRIVAQRPEFKVIISSATINTRVFSRFFDNCPIVSIHSRVYPV
                     HVIYSPAHRQTFDDQLEMIISIVKRESKKQSGDILIFLSGEFEIVTAVNALYAADPKE
                     ELEIYPLYGRLGKEEQERVFTPTSKGKTKVVVSTNIAETSVTIDGITVVIDLGVAKLN
                     FYNQKDFTSSLVPLPTSRSSCEQRSGRAGRTQAGTCYRLFTKEDYQSRPAYQIEEILR
                     TDLSEVVLRMSDLGIYDYEQFSFITRPKAQALKSAEETLRYIGAIDRNRMLTSIGSLM
                     VRFPLLPRHSRVVVEAMMTYPDVLDEVLIAIAFLSTKTPFLFPAGEEDASRAAHRSFN
                     TSAYGDFVMYLNLFRQYANLAKQKQRQDFCTKYYLDHQGMQEIVHVHEQLGEICSEIG
                     FPLSSGGSVREYLSCIAKGLSQYICIRARGTMYKSLSVDQIYIHPGSAWFKTLPRFII
                     AGEIVQTSRLYARSVSPLEQEWLEDIQPGLSKKLMAFDSKQKGKEEEQEEKSFRKRDQ
                     QRGTAGFDLYGKRYPTIKARTKKQQEVVIIPLDDLPSIVKANDRSPQRPKNFRAALAY
                     RGFYVHYNDKFFSLLELNGKLKPELGILDNPPSGVFTIDNARLLIDNLRWLLGFTKSK
                     KNREILGFITLEASGSGNYRFSNNPDFFDALDTSLFHLLHLEDELRDSDRKKEAKEVS
                     SIYTKLLTLAQ"
BASE COUNT          677 a          952 c         1052 g          744 t
ORIGIN      
        1 tgatcgaaat aaccggtggt ctgcatggtt ctcattgtgc tgaaacgatg gtgattgttc
       61 aagggaagga ttgacggttg taagcgaacg ggctttaagg gtataaatag gggtatgaaa
      121 ccactgatga ccgtcacgct cgccggaggc gcccaaaccg aagttcttct tgccgaggat
      181 atcaaagacc tgtcggcgca tctgggcgac tatggccgtt cggtcctgtg ggtcttcgac
      241 tccaacacca acaaactctt tcgccagctt cctcccgcaa gaatcatcct ggacagcggg
      301 gagcaatcga agaaccttac gagccttgag cggatcattt ccctcgccct cgatgaaggc
      361 ctcggccggg acggccgcat catcggcttc ggcgggggag tcgtcgggga catctcctcg
      421 ctcgccgcat ccctctatat gcgcggatgc aggctgaccc tcgttcccac cacgctgctt
      481 gcgatggtcg acgcctcgat cgggggcaag accgctgtgg acttccaggg gatgaagaac
      541 atgatcggga cgttctaccc ggcgaacgaa gtcctcatct cgatcgacgt gctgaagtcc
      601 ttgagcgagc gggagtacaa gagcgggctg gcggaagtca tcaagcatgc cctgctcagc
      661 gaagaccagg agctcttcaa gcttctgctc tcgcgaaggt ccgagatcct caaccgtgaa
      721 gaggagacgc tgatgaagct cgtcgagctt tcgcttctgg tcaagaaatc ctacatcgaa
      781 cgggatccca ccgaaacgaa agggatccga aaagcattga acctcgggca taccttcggc
      841 cacgcgcttg agtcctcaac ccggttcgcc gccttcagcc acggagccgc ggtcgcctgg
      901 ggcaccgtcc gggcccttga agcgggtgtc gccctcggca tcaccgacaa aaagtacgct
      961 tcctccgcct ccaagctgtt cggctccttc gggtacgacc tcgatcatcg gatcggacgg
     1021 ggcgagtgga tcgagtataa gaatcacctc tccaaagaca agaagaagct tggcggagag
     1081 gtccaattcg ttctcttggg cgggaacggc aagcccgagc tgcatccgct tccgtggaat
     1141 ctgatccaag cgctggtgat ggaaaaggct attgggcgag ggtaagcagc ttcgtgtaga
     1201 tcgaagagac ttccttcgcc tccttcttcc ggtcgctatc cctcagttca tcctccagat
     1261 ggagcagatg gaagagcgag gtgtccaacg catcgaagaa gtcggggttg ttcgaaaagc
     1321 gatagtttcc gcttccgctg gcttcaaggg tgatgaagcc gaggatctcc cggttcttct
     1381 tgcttttggt gaagcccaga agccacctga gattgtcgat gaggaggcgg gcgttgtcga
     1441 tcgtgaagac accggaaggc ggattgtcca gaatcccgag ttcgggcttg agctttccgt
     1501 tcagctccaa gagcgagaag aacttgtcgt tgtagtggac gtagaatccc cggtaggcga
     1561 gagccgcccg gaagttcttc ggcctttggg gcgagcggtc gttcgccttc acgatcgaag
     1621 gaaggtcatc caaggggatg atgaccacct cctgctgctt cttggtccgg gccttgatcg
     1681 tgggataccg cttgccgtag aggtcgaagc cggcggtgcc gcgctgctgg tcccgtttcc
     1741 tgaaggattt ctcctcctgt tcttcctctt tccctttctg tttggagtcg aacgccatca
     1801 gctttttgga aaggcccggc tggatatcct ccagccactc ctgttccaac gggctgacgc
     1861 tccgggcgta gaggcgggag gtctggacga tctcgcctgc gatgatgaac cggggcaggg
     1921 tcttgaacca cgccgagccg ggatggatgt agatctggtc gacggaaaga ctcttgtaca
     1981 tcgtgccgcg ggcccggatg cagatgtact gcgacaagcc tttggcgatg caggaaagat
     2041 attccctgac cgacccgccg cttgaaaggg ggaagccgat ctccgagcag atctccccca
     2101 gctgctcgtg gacgtggacg atctcctgca ttccctgatg atcgaggtag tatttcgtgc
     2161 agaagtcctg tcgctgcttt tgttttgcga ggttcgcata ctgcctgaag agattgaggt
     2221 acatcacgaa atcgccgtag gcggaggtgt tgaaggagcg gtgggccgca cggctggcgt
     2281 cctcctcgcc ggcggggaag aggaacggcg tcttcgtcga gaggaaggcg atcgcgatca
     2341 ggacttcgtc cagaacgtcg ggataggtca tcatcgcttc gaccaccacc cggctgtgcc
     2401 ggggcaggag cgggaagcgg accatcagcg atccgatgct cgtgagcatc cggttcctgt
     2461 cgatcgctcc gatatacctc agcgtctctt cggcgctctt cagagcctgg gctttcggtc
     2521 gggtgatgaa ggagaactgt tcataatcat agattcccag atcgctcatc cgcaaaacga
     2581 cttcgcttaa gtcggttcga aggatctcct cgatctgata ggccggccgg ctctggtaat
     2641 cctctttggt gaagaggcgg tagcacgtcc ccgcttgggt ccggcccgcc cggccgcttc
     2701 gctgctcgca gctggaacga ctggtgggca gcggcaccag ggaagaggtg aagtccttct
     2761 ggttgtagaa gttgagcttc gccaccccga ggtcgatgac gacggtgatc ccgtcgatcg
     2821 tcacgctcgt ctcggcgatg ttggtggaaa cgacgacctt cgtcttccct ttggaggtgg
     2881 gggtgaagac ccgctcctgc tcctcttttc caagccgccc gtacagcgga tagatctcca
     2941 gctcctcctt gggatcggcg gcatagagcg cgttgaccgc cgtaacgatc tcaaactcgc
     3001 cgcttaggaa gatgaggata tcccccgatt gcttctttga ttcccgcttg acgatcgaga
     3061 tgatcatctc cagctggtcg tcgaaggtct ggcggtgagc gggggagtag atgacatgga
     3121 ccggatagac ccggctgtgg atcgagacga tcgggcagtt gtcgaagaac cgggagaaga
     3181 cccgggtgtt gatcgtcgcc gacgagatga tgaccttgaa ctccggcctt tgggcgacga
     3241 tccgcttgag caggccgagg atgaagtcga tgttcaggct tcgctcatgg gcctcgtcga
     3301 cgaggatcac cgagtactgc atcagcatcg gatcgttctt cagctccatc aggaggatcc
     3361 cgtcggtcat caccttgatc cgggtggtgt aatcggtggt atcctcgaag cgcatcttgt
     3421 aggcg
//
LOCUS       DAOPUZ010000086         3425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_28154_length_3425_cov_2.996142, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000086 DAOPUZ010000000
VERSION     DAOPUZ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28154_length_3425_cov_2.996142"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKN79_02245"
     CDS             <1..215
                     /locus_tag="PKN79_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ94613.1"
                     /translation="LDFLGGQDMFEAFVPAGAFATGKNLTQYDETINSTWRAQVREYT
                     AGNKTRAQAIADFKRMVFETLDIPSN"
     gene            complement(212..643)
                     /locus_tag="PKN79_02250"
     CDS             complement(212..643)
                     /locus_tag="PKN79_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94614.1"
                     /translation="MALHLEVAVGEPGGKEQSHADLRNKVRCPQNDGVFQACCEGFIV
                     ENRSEVPKQVEISFGSSKKMEFFHTQSPKVGKPDHEGIDDRIEGKHKEGNDKRYGKYI
                     SIFCITHRFFTHDVLPPDKSAVAKKMKLRSGRVLPLLTFND"
     gene            587..1282
                     /locus_tag="PKN79_02255"
     CDS             587..1282
                     /locus_tag="PKN79_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ94615.1"
                     /translation="MALLLTAWFTNRNFKVKGQGAYKILIYMPNIITAATIAILFNSL
                     FGYPKGPVNDLLMKLHIIDGPRNFSLQRWTARGIVSFIQFWMWYGNTMIVLIAGVLGI
                     NPSLFEAAEIDGATPAQIFFRVTLPLLRTILLYVLVTSMIGGLQMFDIPKLFLNGGPD
                     QATVTSSMFIHDQAFSGSYLYNRASAASIIVFGIIIVLSIILFQVLRDKDEVARRKYR
                     RAQNRAARKGVNA"
     gene            1279..2133
                     /locus_tag="PKN79_02260"
     CDS             1279..2133
                     /locus_tag="PKN79_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ94616.1"
                     /translation="MSTHGSIRSKRMLYKTIVYLVCTFLAILSIFPFWIMFMNATRST
                     YEIQQSSIGLVPSKYFMSNWKILTGKAFSPMTGFINSMTVSVGVTVLAVYFSSLTAYA
                     LMAYSWKLRQAFFSFIMAIMMIPAQLSSIGFYQFIYRIGMTNKFTPLIVPAIASPAMV
                     FFMRQYLMAALPLEIVDSARIDGSREFATFNRIILPIMKPALATQAIFSFVGSWNNLF
                     IPLILLTNKKKYTMPIMVSLLRGDIYKTEYGSVYLGLSLTVLPLFVIYFMLAKYIIAG
                     VALGALKE"
     gene            2226..>3425
                     /locus_tag="PKN79_02265"
     CDS             2226..>3425
                     /locus_tag="PKN79_02265"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HNZ94617.1"
                     /translation="MSIHREKAATLLKKMHLEEKVAQLVSVWFEIHDEQTITVREAIP
                     TYNHLPNPMEAALKFGVGQLTRPYGTIANDPQKQVKVINKIQRHLVENTRLGIPAMIH
                     EECLSGVMSVGATAFPCSLNYGSAWDTELMKKIGMAIGDELRSMGVHQGLSPVLDVSR
                     DARWGRLEETFGEDPYLIGEMAIAYINGLQGKNRQPLATLKHFIGHSGSEGGRNHAPL
                     HSGINELLNTYGLPFEKVLKHTAVGGVMPAYHDIDGIPCTSNVELVVDLLKKEWGFDG
                     LIVADYEAIAQLEKDHHVAKDPAEAAALALKAGMDIELPGFTVFKKGLIAAFERKLIT
                     MEEIDRAVMTVLTEKYRQGIFDNPYIDEEGLELSSDEHHALAVEIAQKSLVLLKNEEL
                     LPLQRGKK"
BASE COUNT          821 a          947 c          837 g          820 t
ORIGIN      
        1 cccttgactt cctcggcgga caggacatgt ttgaagcatt cgtccccgca ggcgcattcg
       61 caaccggaaa gaacctcacc cagtatgatg agacgatcaa cagcacgtgg agagcacagg
      121 ttcgcgagta taccgcagga aacaagaccc gcgcccaagc gattgccgac ttcaagcgga
      181 tggtgtttga aacccttgat attcccagca actaatcgtt gaacgtgaga agtggcagaa
      241 ccctgccact tctcaatttc atttttttcg ccactgcact tttatccgga ggaagaacat
      301 cgtgcgtaaa aaatctgtga gttatgcaaa atatggatat atattttcca tatcttttgt
      361 cgttaccttc cttgtgtttt ccctctatcc tatcatctat accctcgtga tcgggtttac
      421 caaccttcgg ggattgggtg tgaaagaatt ccattttctt cgaactgccg aaggaaatct
      481 cgacctgttt gggaacttca gaacggttct caacaataaa accttcacaa caagcttgaa
      541 aaacaccatc attttgtgga cagcgaactt tattccgcaa atcagcatgg ctctgctcct
      601 taccgcctgg ttcaccaacc gcaacttcaa ggtgaagggc caaggcgcct ataaaatctt
      661 gatctatatg cccaacatca ttaccgcagc aacgatcgcc atcttgttca actcgttgtt
      721 cggttatcca aaaggcccgg tcaatgactt gttaatgaaa ttgcatatta tcgacggccc
      781 gaggaacttc agcctgcaac gctggacggc ccggggcatc gtatccttca tccaattctg
      841 gatgtggtat ggaaacacga tgatcgtgtt gatcgccgga gtgttaggaa tcaatccctc
      901 tctctttgaa gcggccgaga tcgacggagc aaccccggcc cagatcttct tccgtgtcac
      961 cttgcccctc ctcaggacca tcctgctcta tgtccttgtc acctcgatga tcggtgggct
     1021 tcagatgttc gatattccga agctcttcct taacggcgga cccgaccaag ccacggttac
     1081 ttccagcatg ttcatccacg accaggcgtt cagcggcagc tacctgtata accgagcctc
     1141 cgcagcgtcc atcatcgtct tcggaatcat cattgtgctc tcgatcatcc tcttccaagt
     1201 tctgagggac aaggatgagg tggcccgacg caagtatcgg agagctcaaa acagagcggc
     1261 gcgaaagggt gtgaacgcat gagcacgcat ggatcgatcc gttcaaagcg gatgctgtac
     1321 aagactatcg tctacctcgt ctgtaccttc ctggccatcc tttcgatctt cccgttctgg
     1381 atcatgttca tgaacgccac ccgttcaacg tatgagatcc agcagtcctc aatcggcttg
     1441 gtgccatcga aatatttcat gagcaactgg aagattctga ccggcaaggc gttcagtccg
     1501 atgaccgggt tcatcaactc gatgacggtc agtgtcggag taacggtttt ggcggtgtac
     1561 ttctcctcgc tcaccgcata tgccctgatg gcatactcct ggaagcttcg ccaggcgttc
     1621 ttctccttca tcatggcgat catgatgatt cccgcccagc tttccagcat cgggttctac
     1681 cagttcatct atcggatcgg aatgacgaac aagttcactc ccctgatcgt tccggcgatc
     1741 gccagtccgg cgatggtgtt cttcatgcgc caatacctga tggccgctct cccgcttgag
     1801 atcgttgact ccgctcggat cgacggatcg cgggagtttg cgacgttcaa tcgaatcatt
     1861 ctcccgatca tgaagcccgc cctagccacc caggcgatct tctcgttcgt cggttcctgg
     1921 aacaacctct ttatcccgtt gattctcctc accaacaaga aaaagtatac gatgccgatc
     1981 atggtcagct tgctgcgggg agacatctat aaaacggagt acggttcagt gtaccttggt
     2041 ctttccctga ccgttctccc cctctttgtt atttacttca tgcttgccaa atacatcatc
     2101 gccggtgtag cgctgggagc gttgaaggaa taaattggct gctgtgtgtc ccgcaacaac
     2161 ccctccgagg gacacacagc aaacctttta tgaagtatga atgtgttgaa gaaggagttg
     2221 tacatatgag catccaccgc gaaaaggcgg caacattact gaaaaaaatg catctcgagg
     2281 aaaaagtcgc ccagttggta tcggtctggt ttgagattca tgacgagcag accatcaccg
     2341 tccgcgaagc gattcctacg tataatcacc tgccgaatcc catggaagcc gctttgaagt
     2401 tcggcgtcgg ccagctcacc cgtccctatg gaacaatcgc caatgatccg caaaagcagg
     2461 tgaaggtgat caacaagatt caacgccacc ttgttgaaaa cacccgtctc ggcattcccg
     2521 cgatgatcca tgaggagtgt ctgagcggag taatgagtgt cggtgcgacg gcatttccct
     2581 gttcgctcaa ctatggctcg gcatgggata ccgagttgat gaaaaagatc ggaatggcga
     2641 tcggggacga gctgcgctca atgggcgttc accaaggctt gtcgccggtt ctcgatgtct
     2701 cccgggatgc ccgttggggg cgtctggagg agacgttcgg cgaagacccc tatctcatcg
     2761 gcgaaatggc gatcgcctac atcaacggtc tccaagggaa gaaccgtcag ccacttgcca
     2821 cgctgaagca cttcatcggc cacagcggca gtgaaggcgg aagaaaccat gctcccctgc
     2881 actcgggaat aaatgaactg cttaatacct acggccttcc cttcgagaag gtgctgaaac
     2941 acaccgccgt cgggggtgtg atgcccgcct atcacgacat cgacggaatt ccgtgcacct
     3001 ccaacgtcga gcttgtcgtc gaccttctta aaaaagagtg gggctttgac ggactcatcg
     3061 tcgccgacta tgaagcgatc gctcaactgg aaaaggacca ccatgtagcg aaagaccccg
     3121 ctgaagcggc ggccctcgcc ttgaaggcgg gaatggacat cgagcttccc ggcttcaccg
     3181 tcttcaagaa aggattgatt gccgcctttg aacgcaagct catcacgatg gaagagatcg
     3241 atcgggcggt tatgacggtt cttaccgaga agtatcgtca aggaatcttc gacaacccct
     3301 acattgatga agaaggtctt gagctaagca gcgacgaaca tcatgcactg gccgtggaga
     3361 tcgctcaaaa gtcgctcgtc ctgcttaaga atgaagaact tctccctttg cagagaggta
     3421 agaag
//
LOCUS       DAOPUZ010000087         3423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_28173_length_3423_cov_3.072447, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000087 DAOPUZ010000000
VERSION     DAOPUZ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28173_length_3423_cov_3.072447"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="PKN79_02270"
     CDS             complement(<1..1324)
                     /locus_tag="PKN79_02270"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ94618.1"
                     /translation="MAYTLYPWQDECIETWLKNRGRGIVRVVTGAGKTILALHAALRL
                     QKTLNENLHIVVVVPKTFLLNQWKNAILSELPISREEIGLVGGGFQNRHAYKVMLYVV
                     NSARSRLSLPLYRHLMADKPVFLIADECHHYTSTENRKIFAFLDSLPTEKKHNYYCLG
                     LSATPKVEPLKNYMGPLIYSYSFSEAIAHKVINRVAIFNVGLRLDPDQAQEYEDLSLK
                     IFKAMRIFLRQNPTFTRLGKAEFYSTLQALANRDDPLAKRLASLLLRRRHLINTAYQR
                     IDAAFDLIALLPGTSRILVFTERIDQSELLYERLKEEYPSQTALYHSELTSDENTLAL
                     RRYQSGEARILVSCKALDEGLDVPSTDVGIVLSTTGEQRQRIQRLGRILRKSRHKAVA
                     SMYYLLVEHTVEDPTLLEEAIETIAEFHLSYDRGTFHHPFYDEVAMPYL"
     gene            1409..2173
                     /locus_tag="PKN79_02275"
     CDS             1409..2173
                     /locus_tag="PKN79_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94619.1"
                     /translation="MDYEQLYEDYLVYEKALKDTVKNVSKLQKSIAKLMDGGDLFRAR
                     TDAIALEESSGEIARLFSSLNARLADFDARAYVEGGDFARQLVDECRRLGVDVIGEFP
                     VYEMFPYKVRIDVENQEVWIDRKKVPGLRPTELARQIRTGQEKLYKVNFKADRFVEEL
                     YSAYELFLLKNKKADGSDVFLDKLYPLLVPMARARKEYDKQAYAFDIARLFEEKELVL
                     KNGKRFQFGPSRNINKALRILDSTGREHFLATIRFF"
     gene            2185..>3423
                     /locus_tag="PKN79_02280"
     CDS             2185..>3423
                     /locus_tag="PKN79_02280"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HNZ94620.1"
                     /translation="MTDEARINATLTQLARGEVPQSEQILGQISVGIEFLTDFWKEQY
                     LKQFIAKGGSKIKFITGRAGSGKSHALSLFTSLAKQEGYLALRLSAKDVWLNDMRDLY
                     LSILKEADIEVLLREAADRIIEQMGHDPKWIEENHTFFDHLAERGLADALTRQELRNQ
                     LRRMFLTNPRMDNNFAIACSLLTGSILGHPILEEENQSLLYRWLAGDRSVRVTMLRPL
                     GLAPSKVTKYNARNLLGSLVELVHVAGYTGLVVAIDDVEVMADASGMNPMRYTKMRRE
                     DAYESIRQLIDDIDTFNHFMVVFGFDRIMLDDEAKGFKSYQALWMRIQNEIKSERINK
                     FADIADLDAIASEVYSPKMVVEMSRRLAEEVRRINIETRVVDEEGARALIERAKSGAV
                     SLPRLVNQATLGSLKEEDHGM"
BASE COUNT          750 a          856 c         1069 g          748 t
ORIGIN      
        1 cgaggtacgg catggccacc tcatcataga agggatgatg aaatgttccc cggtcatagg
       61 agaggtggaa ctcggcgatc gtttcaatcg cttcctccag aagagtggga tcctcgacgg
      121 tatgctcgac taggaggtaa tacatgctag cgaccgcttt gtgcctgctt ttccggagaa
      181 ttctcccaag tcgttgaatt cgctgacgtt gttcgccggt cgtcgaaagc acgatgccga
      241 catcggtgga cggcacatcc aatccttcgt cgagggcttt gcacgagacc agaattcgtg
      301 cttctccgct ctgatagcgc ctgagggcca atgtgttctc atcggaagtc agttcgctgt
      361 ggtagagcgc cgtctggctc gggtactcct ccttcaggcg ttcatacagg agttcgctct
      421 ggtcgatccg ctcggtgaac accaggatcc tgcttgtccc gggcagcagg gcgatgaggt
      481 cgaacgccgc atcaatccgc tggtaggcgg tgttgatcag gtgcctgcgg cgcagcagaa
      541 ggctcgccaa ccgttttgcg agcggatcgt cccggtttgc cagagcctga agcgtggaat
      601 agaattcggc tttgcccagc cgggtgaagg tggggttttg tcgaagaaag atccgcatgg
      661 ccttgaagat cttcagactc aggtcttcat actcttgtgc ctgatcagga tccagacgaa
      721 ggccgacgtt gaagatcgcc acccggttga tcaccttgtg ggcgatcgcc tcggagaagg
      781 agtaggagta gatcaaggga cccatgtagt tcttaagcgg ttccaccttc ggggtggccg
      841 atagcccgag acagtagtag ttgtgtttct tctcggtcgg aagggaatcg agaaaggcga
      901 agatcttgcg gttttcggtg ctggtgtagt ggtggcactc gtcggcgatg aggaagaccg
      961 gtttgtccgc catcagatgg cggtagaggg gaagggaaag acggctgcga gccgagttca
     1021 cgacatagag catcaccttg tatgcatgcc ggttttgaaa gccgccgccg accaacccga
     1081 tctcttcgcg actgatggga agctcggaga gaatggcgtt tttccactgg ttgaggagga
     1141 acgttttggg aacgaccacg acgatgtgaa gattctcatt caatgttttt tgcaatctga
     1201 gtgcggcatg gagggcaagg atcgtctttc ccgcaccggt gacgacccgg acgatccccc
     1261 tgcctcggtt tttcagccag gtctcgatgc attcgtcttg ccatggataa agggtgtatg
     1321 ccatgagcct accgtaccac ggaagaagat cggtgtacca gttgtcagaa gagggattgt
     1381 tcagtaggat tttccccaag gagattccat ggattacgaa caactgtatg aagattatct
     1441 ggtgtatgaa aaagcgttga aagacaccgt gaagaacgtg tcgaagctgc aaaaatcgat
     1501 cgccaagctg atggacggcg gagatctgtt tcgtgcccga accgacgcga tcgcgttgga
     1561 ggaaagcagc ggtgagatcg cccggctctt ttcctcattg aacgcccgac ttgccgattt
     1621 tgatgccagg gcgtatgtcg aaggcggtga tttcgcccgg cagctggtcg atgaatgcag
     1681 acgcctcggg gtggacgtga tcggtgaatt cccggtgtat gagatgtttc cctataaagt
     1741 ccgcatcgat gtggagaatc aggaggtctg gattgaccgg aagaaggtgc cgggtctgcg
     1801 gccgaccgag cttgcccgtc agatcaggac cggtcaggag aagctgtaca aagtgaattt
     1861 taaggccgat cgcttcgtcg aggagctgta ttccgcctat gagctgtttc ttctgaagaa
     1921 caagaaggcc gacggcagtg atgtcttcct ggacaagctg tatcccctcc tcgttccgat
     1981 ggcccgagcc cggaaggagt acgacaagca ggcgtatgcc ttcgatatcg cccggttgtt
     2041 tgaagagaag gagctcgtgc tgaagaacgg caaacgcttt cagttcggcc cctcccggaa
     2101 tatcaacaaa gcgctcagaa ttctcgacag caccggacgg gagcattttc tggcgacgat
     2161 tcgtttcttt tgatggagta ggtcatgacc gatgaggcgc gcatcaatgc aacactcacc
     2221 cagcttgccc ggggggaggt tccccagagc gagcagatcc tgggccagat ttccgtgggg
     2281 atcgagttcc ttacggattt ctggaaggaa cagtacctaa agcaattcat tgccaagggc
     2341 ggaagcaaga tcaagttcat aacgggaagg gcgggcagcg gaaaaagcca cgccctttcc
     2401 ctgtttacat cccttgcgaa gcaggaaggg tatctcgcct tgcgcctgag cgccaaggat
     2461 gtttggctga atgacatgcg cgatctctac ctttcgatcc tgaaggaggc cgatatcgag
     2521 gttctcctcc gggaagcggc cgatcggatt atcgagcaga tgggccatga tcccaagtgg
     2581 atcgaggaga accatacgtt cttcgaccat cttgccgaac gggggctcgc cgatgccctc
     2641 acccggcagg agcttcggaa ccagctgagg cggatgttcc tgacgaatcc ccggatggac
     2701 aacaacttcg cgatcgcgtg ctccctgctg accggttcga tcctcggtca tccgattctg
     2761 gaggaggaaa atcaaagtct tctctaccgg tggctcgcag gagatcggtc ggttcgggtg
     2821 acgatgctcc gtccgctggg gctggcaccg agcaaggtga cgaaatacaa tgccagaaac
     2881 ctgctgggct ctttggtcga attggttcat gttgccgggt acacgggtct ggtcgtcgcg
     2941 atcgacgatg tggaagtgat ggcggacgcc tccggaatga atccgatgcg ctacaccaag
     3001 atgcggcgtg aggatgcata cgagtcgatc cggcagctga tcgacgacat cgacacgttc
     3061 aaccacttca tggtggtctt cgggttcgac cgcatcatgc tggatgatga ggcgaagggg
     3121 ttcaagtcat accaggcgct gtggatgcgg atccagaacg agatcaagag cgaacggatc
     3181 aacaagttcg ccgatatcgc cgacctggat gcgatcgctt ccgaagtgta ttcgccgaag
     3241 atggtcgtcg agatgagccg ccggctcgcc gaagaggtcc ggcgaatcaa catcgagacg
     3301 agggtcgtcg atgaagaggg ggcgagggcc ctgatcgagc gggcgaagag cggggcggtg
     3361 tcattgccga ggcttgtcaa tcaggcgacg ctggggtcgc ttaaggagga agatcatggc
     3421 atg
//
LOCUS       DAOPUZ010000088         3405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_28351_length_3405_cov_3.382388, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000088 DAOPUZ010000000
VERSION     DAOPUZ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28351_length_3405_cov_3.382388"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..1375
                     /locus_tag="PKN79_02285"
     CDS             380..1375
                     /locus_tag="PKN79_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ94621.1"
                     /translation="MRKVLLILGVLLASATMLFSAGQADKAVETGPSKVSMYANITAI
                     EPILERYKAESGVEVEYTRLSTARYVSTILTEVEAKKLVADVVQGPLPVLEILREYGV
                     LSNYEAKAMEGYPDWAKKDGIHLFGIEYVGLIYNKDLVSAAEAPKSYMDLTDPKWKGH
                     IVMPDPGAHATTISWLVGLRENVFKSDAEWYRFLEGLAKNEPMLVASFSPTANPLASG
                     EKKIGVSMPKYIVTKAPAPLDYAKISPMLGSPRAIAITKDAPHRKDAEAFLEFWLGKS
                     AASQLANDVGEYVLAPGVYPPIPGIAKTEVIPIRELTDEEIQKWGTEFGKIFALK"
     gene            1403..2485
                     /locus_tag="PKN79_02290"
     CDS             1403..2485
                     /locus_tag="PKN79_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ94622.1"
                     /translation="MEINVRGLKKYYQSEGKVIKALDGVDLTIEANKIFTLLGPSGCG
                     KTTLLRCIVGLETPDEGEIRIGETIVWSKEKGLYVPPEKRELGMVFQTYAIWPHMNVF
                     NNVAYPLQNMKLPKDEIADRVAKTLKFVQLEGFENRPSTRLSGGQQQRVAIARALVAE
                     PKVILFDEPLSNLDAKLREETRKELRVFLTELQITAVYVTHDRVEALALSDMIGVMNK
                     GKIVELGTPRKIYFDADHRFVADFIGRSNLIPAKLLRQEKLHTIVETPIGEIACHKRT
                     FEQDAELVLCLRPEFINTEKPSDEHTNRVTGIIRQMTFVGEVYEADVRIGDETVLVKL
                     DPETTLVEGDELTLSMKPDHCLLVSR"
     gene            2495..>3405
                     /locus_tag="PKN79_02295"
     CDS             2495..>3405
                     /locus_tag="PKN79_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ94623.1"
                     /translation="MEKRNARLTYSLIVIVSLLTVAPVIMLVIGSFSNNLNSFGNFTL
                     KKYAAVYTDPMLIDILGDTAAFVLGSALFATVLAIFLAYLNNRTDIPFKFLFKIVSVM
                     PMMIPHVLFAVSWALLLNPTNGILNMWLTRLFALPKAPFNIYTIPGMIVVEGLLDLPV
                     AYLIISPAMGSFDATLEESSRVFKAGPLKTLFRITLPVLRPAIMASFILCVVRALASF
                     AVPSVLGMPGRIYVLSTFIYRVVSMSLIADYGKAAAIGMSILVISISLIYVYRYYTRA
                     SDKFVTVSSRGFKPTVVELRKGRIPLF"
BASE COUNT          820 a          865 c          917 g          803 t
ORIGIN      
        1 ggattgtttg aatcgcacgg agaccgccat ctctcaggaa cacattttca gggcgatcga
       61 actggccatc gaggctcaac ggaaagcgat caatattaca agagcctgat gatatgaggg
      121 aaatcccgat ttgtcgagaa tcggatacag atcggtgata tcgaactcaa aagcttgggt
      181 tcgtagcatg agactgtacc tttcaacgtt taggtattcc taaccgaccg gaggcaatcg
      241 gttctgttcg cctgaattcc ttccggagct ccttctttta gggtataatt cttttccttc
      301 agggtatact tcttttcatt tgaccgaagt atgggggcat ggtacgcttt ttttacatac
      361 taatggagga tatatgaata tgagaaaagt attactgatt cttggcgtgc ttcttgcatc
      421 cgcaacgatg ttgttcagcg cgggacaagc ggataaagcc gttgaaaccg gtccttcgaa
      481 agtttcgatg tatgcgaaca tcacggcaat tgaaccgatt cttgagcgct acaaggcgga
      541 aagcggagtg gaagtggagt atacccgact ttccaccgcc cgttatgttt cgacgatcct
      601 gaccgaagtc gaggcaaaaa agctggtagc ggacgttgtg cagggaccgc ttccggttct
      661 cgaaatcctg cgtgaatacg gcgtgctttc aaactacgaa gcgaaagcga tggaagggta
      721 ccctgattgg gcgaagaaag atggaatcca tctttttgga atcgaatatg tcgggctcat
      781 ctacaacaag gatcttgttt ccgccgccga agctcccaag agctacatgg atctgaccga
      841 tcccaagtgg aaagggcaca tcgtgatgcc tgatcctgga gcccatgcca cgacgatcag
      901 ctggctcgtc ggattgcggg aaaacgtctt caagagcgat gcggagtggt atcggttcct
      961 tgaagggttg gccaaaaacg agccgatgct cgtcgcttcg ttcagcccga ccgccaatcc
     1021 gcttgccagc ggcgagaaga agatcggcgt ttccatgccg aaatacatcg tgacgaaagc
     1081 tccggcaccg cttgactatg caaaaatctc tccgatgctg ggttcgcccc gagccatagc
     1141 gatcacaaag gatgctccgc acaggaagga cgctgaagcg ttcctggagt tctggctggg
     1201 caagagcgcg gcaagccagc ttgcaaacga cgtgggtgaa tatgttctcg ctccgggagt
     1261 atatccgccg atcccgggaa tcgccaagac ggaagtgatt ccgattcgcg agcttacgga
     1321 cgaggaaatc caaaaatggg gcaccgagtt cggcaagatc ttcgcattga agtaagaaac
     1381 acatcgaggg cggaaacacg atatggaaat taatgtcagg ggattgaaga agtactatca
     1441 aagcgagggt aaagtgatca aagcgctcga tggcgttgac ctcaccatcg aggcgaataa
     1501 gatctttacg cttcttggtc cgagcgggtg tgggaaaacc accttgcttc ggtgcatcgt
     1561 cggactggaa actcccgatg aaggagagat ccggatcggg gagacgatcg tctggtcaaa
     1621 agaaaaaggc ctttacgtgc cccctgaaaa gcgcgagctg ggcatggtct tccagaccta
     1681 tgcgatctgg ccgcatatga acgtcttcaa caatgtcgcc tatccgcttc agaacatgaa
     1741 gcttccaaag gatgaaattg ccgatcgtgt cgctaaaacc ctcaagttcg ttcagttgga
     1801 ggggttcgaa aaccgcccct ccacccggct ctccggcggg cagcagcagc gtgttgctat
     1861 cgcccgggcc cttgtcgccg aacccaaggt gattctgttt gatgaaccgc tgtcgaacct
     1921 tgatgcgaag ctccgggaag aaacccgtaa agaactgaga gtattcctga ccgagcttca
     1981 gatcaccgca gtctatgtta ctcacgaccg agtcgaagct ctcgcccttt ccgatatgat
     2041 cggggtgatg aacaaaggaa aaatcgttga gcttggtaca ccgaggaaga tctactttga
     2101 cgctgatcac cggtttgttg ccgacttcat cggcaggtca aacctgattc cggccaagct
     2161 cctccgccag gagaagctgc acacgatcgt ggagactccg atcggtgaaa tcgcatgcca
     2221 caaacggacc tttgaacagg atgccgagct ggttctttgt ctgagaccgg agttcatcaa
     2281 caccgaaaaa ccgagcgacg aacatacgaa ccgggtaacg ggaatcatcc gacagatgac
     2341 gttcgtcggc gaagtctatg aagccgatgt ccgaatcgga gatgaaaccg ttctggtgaa
     2401 gctggatccg gaaaccacac ttgttgaggg agatgaattg acgctgagca tgaaaccgga
     2461 tcattgtctt ctcgtttcac ggtaggaggg cagtatggaa aagagaaacg cccgtcttac
     2521 ctattccctc atcgtcatcg tcagcttgct caccgtggcc ccggtgatca tgctggtcat
     2581 cggaagcttc tcgaacaacc tgaattcgtt cggaaacttt accctcaaga aatatgcagc
     2641 cgtttatacc gacccgatgc tcatcgacat tctgggggac actgcggcgt tcgtgttggg
     2701 ttcggcactg ttcgccaccg tgttggcgat ctttctggcg tatctgaaca accgcaccga
     2761 tattcctttc aagtttctct tcaagatcgt atcggtgatg ccgatgatga ttccgcacgt
     2821 gttgtttgct gtcagctggg cgctgttgct caaccctaca aatggaatcc tcaatatgtg
     2881 gctgacgaga ctctttgcgc ttccaaaggc tccattcaac atctacacca ttccggggat
     2941 gattgtcgtc gaggggctgt tggacctgcc tgttgcctat ctgatcatct caccggcgat
     3001 gggatcgttt gatgccacgc ttgaggagtc gtcgcgggtt tttaaagccg gaccgcttaa
     3061 aaccctcttc aggatcacgc tgccggttct tcgcccggcg atcatggcgt ctttcattct
     3121 ctgtgtcgtt cgagcgctgg catcgttcgc cgttccttcc gtcttgggaa tgcccggtcg
     3181 gatctacgtg ctgtcaacgt tcatctatcg ggtcgtttcg atgagcctga tcgccgatta
     3241 cggaaaagcc gcggcgatcg gaatgagcat cctcgtaatc agcataagcc tgatctacgt
     3301 ctatcgctac tacaccagag caagcgacaa gttcgttacc gtcagcagtc gcggtttcaa
     3361 gcccaccgtg gtggagctga gaaagggaag aattccgctt ttcat
//
LOCUS       DAOPUZ010000089         3401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_28397_length_3401_cov_2.375672, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000089 DAOPUZ010000000
VERSION     DAOPUZ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28397_length_3401_cov_2.375672"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..636
                     /locus_tag="PKN79_02300"
     CDS             382..636
                     /locus_tag="PKN79_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ94624.1"
                     /translation="MTLGQVIKHLRYKKNMTQEQLIEKAKLTRSQLYYIEADRRGIHL
                     TTLFAICNGLGVSLVEFVSYLDDPFTPSISSIEAPGATIG"
     gene            complement(570..2248)
                     /gene="ilvB"
                     /locus_tag="PKN79_02305"
                     /pseudo
     CDS             complement(570..2248)
                     /gene="ilvB"
                     /locus_tag="PKN79_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
     gene            complement(2252..>3401)
                     /locus_tag="PKN79_02310"
     CDS             complement(2252..>3401)
                     /locus_tag="PKN79_02310"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNZ94625.1"
                     /translation="DDAELLDWENNSCPTCGSCSGMYTANSMNCLTEAIGMGLPGNGT
                     IPAVYAERDRLAKEAGYRIMELLKEDVRPLDIMTEKAFENALAVDMAIGCSTNTMLHL
                     PAIASEAGIDLDIFIANEISAKVPNLCHLAPSGNDHLEDLYHAGGVLAVMHELEPLGV
                     LHGDLPTVSGKTIGRLWAEHPVRNHEVIRPLDNPFDKTGGIAVLKGNLAVDGAVVKRS
                     AVDPSMLVHSGPARVFDSEEEANEAIFSGGINKGDVIVIRYEGPKGGPGMREMLSPTS
                     AIAGMGLDKDVALITDGRFSGATRGSSIGHVSPEAASGGLIALVEEGDEILIDIPGGE
                     LTLKVDEAILNKRRASFKEKEVVRYGGYLDRYRKQVTSANTGAIFAQE"
BASE COUNT          693 a         1025 c          912 g          771 t
ORIGIN      
        1 gagcccgatc tcctgaagtg tcgcctgtcg tccgggcttt tccgttttct ctgaaaaata
       61 cgttgcaaga cgaccaaaca aggcggcttc ctcacttcga agctgctttt cacacgcctt
      121 ctgcagattc gtgcacatca ccgtcagctc accatgattg agcgtaccca tatacgcacc
      181 tcctccgata gatacttcag tataccaaga gaagattgat tgttgggaaa aatctaccgc
      241 tatctgacgt attttaggaa tcatcagtca attatttagc tgtttatgca acaattttga
      301 tgaccgaagc tcatactcct ggtttatgac ataaacctgt caggttcatt ttttctttga
      361 catactatcg gaatggaaaa gatgacactt ggacaagtta tcaagcacct gcgttataag
      421 aaaaacatga cgcaggagca gttgattgaa aaagcaaagc tgacgcgcag tcagctttac
      481 tatatcgaag cggacaggcg gggcattcac ctgaccaccc tctttgcgat ctgtaacggg
      541 ttgggagtat ctctcgtgga attcgtctcc tatttggatg accccttcac cccaagcatc
      601 tcttcgatcg aagctcccgg ggcgaccatc ggataggctt tatcatcggt aggaatttcg
      661 caatcgatta caaccgcctc attcagcgca aacgcttcct tgagaatcgg aagggaatcc
      721 tgctccctgg cgatccgcat tcccttgaga ccgtacgcat tcgccagcat cacccagtcg
      781 atcggggtgt cgagtgtcgt ttcgctgtag tgtgaattga agaagagggt ttgccactgg
      841 cggaccatcc cgagggtccg gttgttcatg atcagaatga ccaccggcaa acgataccgg
      901 gcgatcgtgg caagctcgtt gcagttcatc ttgaaggaac cgtcgccggc tacgctgacc
      961 actcgagctt cggggttgcc cacctgagcg ccgatcgcag agcccgttcc gtagcccatc
     1021 gttcccaagc ccccgctggt gaggaagtga ccgcactgcg tatgcttgag gaactgggca
     1081 gcccagatct ggtgctgacc gacctcggtg accacgaaag cgccttcagg cagaaccttc
     1141 tgaagcgcct tgaggacctc cttgctccgc tggttctcgc tttcgatccg gatcggaaaa
     1201 cgctctttgt aggaatatac ctggtccatc cacccattgg aggaaatctg cttgtcgatt
     1261 cttcggttga gctccttcag aacaatccgg acatccccga cgattccgac ggtggcggtg
     1321 atgttcttgt cgatctccgc cggatcgaca tcgatatgga tgatgtttgc tccccgggcg
     1381 aacgctgatg ctttgctgac gacccggtcg ctgaagcgcg agccgatcac aatcagcagg
     1441 tcgcacatcg agacgatcat gttggagacc ttcgtcccat gcattccgac cattcccgta
     1501 tagcgtgtgc tggtcgtcgg aatcgctccg atccccatca tcgaagcgca caccggagca
     1561 tcgatctttt cgatgaactc ggtaagctct ttgctcgccc gagcccgaac gactcccccg
     1621 ccgacaaaac acaccggccg ctcagcgcta tgaataagct caagcgcggc gctgagcgcc
     1681 tgttcactga tccggtccga ccggggtttc atcggctcaa tgacgtggcg ctcatactcg
     1741 gcgtactcga ccgttacatc cttcggcaca tcgatcagga cggggcccgg ccggcctcct
     1801 gggcgatttg gaacgccaga cgaatcgtcc tggcaagatc gttcacatcc ttgacgatga
     1861 agttgtgctt cgtcaccggc attgtgattc cggtgatatc gacttcctgg aaggaatcct
     1921 tcccgagaag gggaaccgtc acgttcccgg tgatcgccac gaccggcacg ctgtccatat
     1981 aggcggtggc gattcccgtc accaggttgg tcgcccccgg tccgctggtt gcgaacacca
     2041 ccccgacctt gccggttgag cggctgtagc cgtcggcggc atgggcagcg ccttgttcat
     2101 ggctggtgag aatatggttg atacgatcgc gataggaata gagggcatca tagaggggaa
     2161 gaaccgcccc gccgggaaac ccgaagaccg tatcgacgct ctgttcaagc agacattcca
     2221 aaatgatttc ggcaccacgt aatttcatcc gtcactcctg ggcaaaaatt gctccggtgt
     2281 tcgcactggt cacctgcttt cgatagcgat ccaggtaacc gccgtagcga acgacttctt
     2341 tctccttgaa ggaggcacgg cgtttgttca atattgcctc atccaccttc aacgtcaact
     2401 ctccgcccgg gatgtcgatg aggatttcgt ccccctcctc gacgagggcg atcaggcccc
     2461 cgcttgccgc ctcggggctg acgtggccga tcgaggaacc cctcgtcgct ccgctgaagc
     2521 gcccgtcggt gatcagggcc acatccttgt ccagacccat gccggcgatc gcgctggtgg
     2581 gactgagcat ctcccgcatc cccggacctc ctttggggcc ttcatagcgg atgacgatca
     2641 catcgccctt gttgattccg ccggagaaga tcgcctcgtt cgcctcctct tcgctgtcga
     2701 agacccgggc cggcccgctg tggaccagca tcgagggatc gacagcgctc cgtttgacca
     2761 ccgccccgtc gacggcgagg ttccccttca gcacggcgat accgccggtc ttgtcgaagg
     2821 ggttgtccag cggtcgaatg acctcatggt ttcgcaccgg atgctccgcc cacaaccggc
     2881 cgatggtttt accgcttacc gtcggcagat ccccgtgaag gacgccgaga ggttccagct
     2941 catgcatcac cgcaaggacg ccgccggcgt gataaagatc ttccagatgg tcattcccgc
     3001 tcggagccaa atggcagaga ttgggcacct tggccgagat ctcgttggcg atgaagatat
     3061 ccagatcgat tcccgcttcg ctggcgatcg ccgggaggtg cagcatcgtg ttggtcgagc
     3121 aaccgatcgc catatcgacg gcgagggcgt tttcaaacgc cttctccgtc atgatgtcca
     3181 gcggcctgac atcctctttg agcagctcca tgatccgata tcccgcttct ttggcaagcc
     3241 gatcccgctc ggcatagacg gcgggaatcg tgccgttccc cggaaggccc attccgatcg
     3301 cctcggtgag gcagttcatg ctgttggcgg tgtacatccc cgaacaggag ccgcaggtgg
     3361 gacatgagtt gttttcccag tcaagcagct cggcatcgtc g
//
LOCUS       DAOPUZ010000090         3381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_28627_length_3381_cov_2.758870, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000090 DAOPUZ010000000
VERSION     DAOPUZ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28627_length_3381_cov_2.758870"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKN79_02315"
     CDS             complement(<1..405)
                     /locus_tag="PKN79_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ94626.1"
                     /translation="MRFVGRESELKEFHEIFTSSRFEFVVVYGRRRIGKTTLISKACE
                     GHRTIFHLAAKTTGTKTLSDLSTAAAAGLDEGFGIGFPTLEVFLEYIAEKAKEEPLIL
                     VIDEFPFFCASIPEAMGVFQRCIDLRFPKTRLK"
     gene            841..2208
                     /locus_tag="PKN79_02320"
     CDS             841..2208
                     /locus_tag="PKN79_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNZ94627.1"
                     /translation="MKVLIFGLGLHGGGYAAASYFLDHGHEVRITDLRAEEDLKNQVA
                     SLVNRGATYSLGNHRKDDFLWADVVVKNPAIPPSHPFLKDARVVINDFSWLFSSPWIQ
                     EVKIIAITGTKGKTTTSAAVAHVLAHLGVEAMQCGNMGISGFTVLKEFEDRRKQGRDL
                     PVYLVCEFSSWQIRDTIVALAGAMPHLEICALTSFYPDHLNTYESIERYMEDKLLLFG
                     PFVKKAVIPQIIARRVRQQTGLDRKRIRPSDTTVPKELIEHPNLHSAYAILLSLGFKY
                     REVLEALGSFTGVPHRIEAIGSLRNILFINDSAATIPEAVHFTYGNFKLLAVHLITGG
                     TDKRLDPNSMVEELRQALSVSLLGGSFTTDKLIPLLERENIPYFGPFDSMGAALEASL
                     DSALKKERELPDLQQVILLSPGSASFELFDNEFDRGDQFRTLVNLLILAEETARTDTD
                     GAPEP"
     gene            complement(2153..3088)
                     /locus_tag="PKN79_02325"
     CDS             complement(2153..3088)
                     /locus_tag="PKN79_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNZ94628.1"
                     /translation="MKIKPIRAEELTGEDSPLQSLFWARAKSILGWKPYAFEINEEQR
                     LLLLVRRIAPLFFFAYVPFGPSKPVDLSMLSEGLKQAIDEPVFAIRYDLPFTDTAFET
                     GGLLSLSYSIQPERTVRIDLQKPLVYRKRALRALKKCENRYTVRPYRGEPILFEQWYE
                     TYRQTALRDRFHPRSKEYFFHLLSIDEDKMVKPILLLAFEQEKVVGGVLLIEGKKETI
                     YLAGSSTRPDVGYLLQDGAIRRTKERGGELYDLFGISNQSGYLASLDLFKTAFGGEIV
                     KREPSLDYPIRALRYRLFTVLEHHRYRFSRSLQQG"
     gene            complement(3095..>3381)
                     /locus_tag="PKN79_02330"
     CDS             complement(3095..>3381)
                     /locus_tag="PKN79_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNZ94629.1"
                     /translation="RGVKSRGMLLAANDPEAEEHTTCEVIFVDDFETGTILEVEGFDP
                     PQADIPYVKPDLFFSFELYAEDGVVKVDGRPIGKAGRHLRTVTYLNGPVN"
BASE COUNT          808 a          919 c          852 g          802 t
ORIGIN      
        1 cttgagtcgg gtcttgggaa agcgtaaatc gatacatcgc tgaaacaccc ccatcgcttc
       61 ggggattgat gcacaaaaaa acggaaattc atcgatgacg aggatgagcg gctcttcttt
      121 ggccttctcg gctatatact ccagaaagac ttccaatgta ggaaagccaa taccgaatcc
      181 ttcatcgagc cccgctgcgg cagccgttga aagatcggaa agggttttcg tcccggttgt
      241 ttttgccgcc agatgaaaga tcgttctgtg cccttcacat gctttggaga tgagggtcgt
      301 ttttcctatc cgacggcgac catagaccac tacaaactca aaacgggacg aggtgaagat
      361 ctcatggaac tctttcagct cggattctct tccgacaaat ctcatcgctt atccttccct
      421 ccgttcggtg tatccccaga gtagcagaag aaatggaagc aatcaagtca ttcaaatacg
      481 ttttaataaa atcgatttga ataaaactct cctatcaagg ttgaaactca gcagtttctt
      541 gtgtatgggt accgcgcaaa atgggtgtct gaaaagtgga accttttaac ccgttgctga
      601 caaattgata ttcaatgata gaaatggcat ggatatccgt acacttctac agcctgatcg
      661 aacatcaatc acagagctta tacgggaact gtcagcctcc tgccggaaca gggcgcatgg
      721 tttcccacat agcaaaccat gtacgataaa ggacggagca aatatctgtt ttagcgctcc
      781 attcgatgat tttacttgca ctctccccct cttcttccta cactgtcctc agggggtagg
      841 atgaaggtat tgatcttcgg tttggggctt catggcggtg gatatgctgc ggcatcctat
      901 tttcttgatc atgggcacga agtacgaatc acggatctgc gggcggaaga ggatttaaag
      961 aaccaagtag catccctcgt gaatcgaggg gcgacgtaca gtttgggcaa tcatcgaaaa
     1021 gacgattttc tctgggccga cgtggtggtg aagaatccgg cgattcctcc ctcccatcct
     1081 tttctgaaag acgcccgggt cgtgatcaac gatttttcct ggctgttttc atccccatgg
     1141 atccaggaag tgaaaatcat cgccatcacg ggtaccaagg ggaaaaccac gaccagcgcc
     1201 gccgttgctc acgttctcgc ccacttgggg gtggaggcga tgcagtgcgg gaacatggga
     1261 atcagcggat ttaccgtcct caaagagttt gaagaccgaa ggaagcaggg aagagaccta
     1321 cccgtctact tagtctgcga gttctcttcc tggcagatca gagacacgat cgtcgctctt
     1381 gccggggcga tgcctcatct ggagatctgc gctctgacct ccttctaccc cgatcatctc
     1441 aacacgtatg aatcgatcga acgctatatg gaagacaaac tgctcctctt cggtcctttc
     1501 gtgaagaaag cggtcattcc tcagatcatc gctcgacggg taagacaaca aaccggtctg
     1561 gaccgcaagc gcatccgacc gagcgacacc accgtaccca aagagctcat tgaacatccc
     1621 aacctccaca gtgcgtatgc gattctcctc tcgctgggct tcaagtaccg ggaagtcctc
     1681 gaagcgctcg ggtcgttcac cggggttcct caccggatcg aagcgatcgg aagcctcagg
     1741 aacatcctct tcatcaacga cagcgccgca acgatccccg aagcggttca cttcacctat
     1801 ggaaacttca aacttctggc ggttcatctg atcaccgggg gaaccgacaa gcgcctcgat
     1861 cccaattcca tggttgaaga gcttcgtcaa gccttgagcg tcagtctcct ggggggaagc
     1921 ttcaccaccg acaagttgat tcctttgctc gaaagagaga acattccata cttcggcccc
     1981 ttcgattcaa tgggagcggc gcttgaggcg agcttggaca gcgccctgaa aaaggaacgg
     2041 gagcttcccg atttgcagca ggtgattctg ctctcgccgg gaagcgcttc ctttgagctg
     2101 ttcgacaatg agtttgaccg cggcgaccag ttcaggacac tggtgaatct cctcatcctt
     2161 gctgaagaga ccgcgagaac cgataccgat ggtgctccag aaccgtgaaa agccggtacc
     2221 gtagagctct gatcgggtag tcaagagacg gctcgcgttt gacgatctct ccgccgaatg
     2281 cggtcttgaa cagatccagg gaagcgagat acccgctttg gttggagatt ccgaagagat
     2341 cgtagagttc accgccccgt tcctttgttc gccgaatcgc tccatcctgc agcagatacc
     2401 ctacatcggg cctggtcgat gatcctgcga ggtagatcgt ttctttcttc ccttcgatga
     2461 gcagcactcc cccgaccact ttttcctgct cgaaagccag aagaaggatc ggtttcacca
     2521 tcttgtcttc atcgatcgaa aggaggtgga aaaagtattc tttgctccgc gggtggaacc
     2581 ggtcgcgaag agcggtctgc ctataggttt cataccactg ctcaaagaga atcggttcac
     2641 cccggtaggg tcggaccgtg tacctgttct cgcacttctt aagcgccctc agagcgcgtt
     2701 tgcgatagac aagcggcttt tgaagatcaa tccgaacggt ccgctccggc tggatcgaat
     2761 aggataatga taaaagacct cccgtttcaa atgcggtgtc cgtgaacgga aggtcgtagc
     2821 ggatcgcaaa aaccggttca tcgattgcct gtttcaaacc ttcggataac atcgataagt
     2881 cgaccggctt gctcgggccg aagggaacat atgcgaaaaa gaagagcgga gcgattctgc
     2941 gaacaaggag cagcagccgc tgctcctcat tgatttcaaa agcgtagggt ttccacccga
     3001 ggatcgactt tgcccgagcc caaaagagcg attgaagcgg agaatcttct ccggtgagtt
     3061 cttctgctct gatcggcttg atcttcatac acctttagtt taccggaccg ttgaggtatg
     3121 tgacggttct caggtgcctg cccgcttttc cgatcggtcg tccgtcaacc tttacgaccc
     3181 cgtcttccgc atacagctca aaggaaaaga agaggtccgg cttcacatag ggaatatccg
     3241 cctgtggcgg atcgaatcct tcgacttcca ggatcgtccc cgtctcaaaa tcatcgacaa
     3301 agatcacttc gcacgtggtg tgctcttccg cttcgggatc gttcgccgcc aacagcattc
     3361 ccctgctctt caccccccgg a
//
LOCUS       DAOPUZ010000091         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_28874_length_3360_cov_2.942209, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000091 DAOPUZ010000000
VERSION     DAOPUZ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28874_length_3360_cov_2.942209"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..613)
                     /locus_tag="PKN79_02335"
     CDS             complement(86..613)
                     /locus_tag="PKN79_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HNZ94630.1"
                     /translation="MFKFFSITQHVKEQEYLRSMHQLGWKLVKTTGIGMYHFEACEPE
                     DVIYQLDYNAEGLANKDEYVRMFEDCGWEYIQEFVGYSYFRKPAAKGTAKDEEIFCDD
                     ESRAQMMERVFKGRLIPLLVIFFVVFIPQFFMNVTTRKSLGIAITLGAMLLVYMSIFV
                     TFTVQYVKLKNRTKR"
     gene            complement(606..941)
                     /locus_tag="PKN79_02340"
     CDS             complement(606..941)
                     /locus_tag="PKN79_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014654251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNZ94631.1"
                     /translation="MNEYIKKIYVPMTESAFFILLCLQKENHGYGIVKRVEELTDGEV
                     ILAPGTMYGSLSKMEKDGIIRFVRNEDKRKIYIITDLGLKVLKTELKRIERLYHISRE
                     VPENDEEGV"
     gene            1263..2960
                     /locus_tag="PKN79_02345"
     CDS             1263..2960
                     /locus_tag="PKN79_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNZ94632.1"
                     /translation="MVKLPKMRRRKNRSFAYRGRRLLHIDLEKETSHLIELSEEESNQ
                     LIGGRLLALSLWERFVSFDKLEPSFFEAHNPLVLAPALAADLEPESCGSMTLVTKDPQ
                     RGKLAVSTVQTSFSTALLNCGYSAVVFTGRCRRLTGVTLDHDQVEFFDAESFHDLPTG
                     ETKHRAKIEHILCIGPAAERQIPHCSICINNQNIPFSSALGLVMGLKNVKLLGLVPHV
                     SGREGYDPGRLGIFKKRLEQSLRFSALAQRVHEEGDVRLLEKANTFGWAAIGTHRYRY
                     DLRLVHLYERFDGRADTYSLIEALALGANLEIFEPSRIAQLARRCKENGLDPASVGVL
                     LGWARVARKEGTLSFLPDLQSRLVANYLKVLDAISYGKGSGHQLGKRLDQLVAEYGGK
                     NGCYCIGSQPLGPFDYRALPTQALLVSMGDHRIVLGELIRGGHYRRGKAKRSAAWALY
                     LQRLWGAQESVGLSPYVDLPFFGRWYPHIKGRRSRFAMSALIASISEGKTFTAQHLAS
                     VGKRAWSLEREINRRLGWQDPPFAEQQLLDGRSNFPKSQVVPLALLRAAYHELLKRW"
     gene            complement(2936..>3360)
                     /locus_tag="PKN79_02350"
     CDS             complement(2936..>3360)
                     /locus_tag="PKN79_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ94633.1"
                     /translation="QVLIAGDLCPREEPVFASLLSRLPGLVLVRGNSDVSYQFAQARI
                     HLPPLVRTLSYQGLPITLTHGHLDVPYTVGGIVISGHTHIPHLKKDADGTLWLNPGSP
                     SRPRSSSGATYALIDTEGVGIHRLKTHSPFLYHRFKSS"
BASE COUNT          739 a          905 c          870 g          846 t
ORIGIN      
        1 ttgtcatcgt tctgcgccaa ctgataattg atgtccgaaa accggatggt cttttcactt
       61 tgcatggcac gctgtcatac gatattcatc ttttcgtcct gttcttcagt ttcacgtatt
      121 gcacggtgaa cgtcacaaaa atcgacatat atacaagaag cattgcacca agggtaatgg
      181 caattccaag gcttttgcga gtcgtcacgt tcatgaagaa ctgaggtatg aagaccacga
      241 aaaagatgac aagcaatggg atcagcctcc ccttgaaaac acgttccatc atctgagccc
      301 tcgattcgtc atcacagaag atctcttcat ccttcgctgt gccttttgcc gcaggttttc
      361 tgaaatagct gtatccgacg aattcctgga tatattccca gccgcaatct tcgaacatcc
      421 ggacatactc gtctttgttg gccaatccct ccgcattgta atcgagctgg tagatcacgt
      481 cttccggttc acatgcttca aaatggtaca tgcctatccc ggtggttttg accagcttcc
      541 agcctaactg atgcatgctc ctcagatatt cctgttcctt tacatgctgg gtgatcgaaa
      601 aaaacttaaa caccttcttc gtcattctct ggtacctccc gggagatgtg atataagcgc
      661 tcaatccgtt tgagttccgt cttcaggact ttgagcccca aatcggtgat gatatagatc
      721 ttgcgcttgt cttcgtttcg aacgaaccgg atgattccgt ccttttccat ttttgagaga
      781 cttccgtaca tcgttccggg cgcaagaatg acttcgccgt cagtcaattc ttcaaccctt
      841 ttgacgattc catagccatg attttctttt tgcaggcaca gcaggatgaa aaatgcgctc
      901 tcggtcatcg gcacatagat cttttttatg tattcattca tagctcctcc cacgctgtct
      961 tgaagtacga tgtattgtgt tacgatatat cgtacttcga tatataatat atcgaggttc
     1021 gatatatgtg tcaagaggtc tttgatgatt atttgatcga gtattcgtat agatgcgatt
     1081 tgaggtgtaa cccgcaggtt gcatggttcc ccaacggtga tttcagggct ctcttttgcc
     1141 cgccttcata ccgacatatt tgccaaccct ttcttcccct gcagcataat gatgtcatcc
     1201 actccaatcg ctctcgtcta agaactttca cttgtggtat actccctgca gagaggatgt
     1261 tcatggtgaa gttgccgaag atgaggagac ggaagaatcg atcctttgcg tatcgcggac
     1321 gcagactcct tcacatcgat ctggagaaag aaacatcgca cctgatcgaa ctctccgaag
     1381 aagaatcgaa tcagttgatt ggcggcagac tgctcgctct ttcgctttgg gagcggtttg
     1441 tttcctttga caagcttgag ccgagttttt tcgaagcgca taaccccctt gttttagccc
     1501 ctgccttggc agcggatctg gaaccggaga gctgcggatc tatgaccctc gtcaccaagg
     1561 atccgcagcg cggcaagctt gccgtctcga cggttcaaac atccttttcg accgccttgc
     1621 tgaactgcgg ttattcggcc gtggtgttca ccggtcgttg ccgccggttg accggcgtta
     1681 cacttgatca tgatcaagtg gagttcttcg atgccgagag cttccacgat cttcccaccg
     1741 gagagacgaa gcaccgggcg aagatcgagc atatcctctg catcggaccc gctgccgaac
     1801 ggcagattcc ccactgttcc atctgcatca acaatcagaa cattcccttt tcctccgcct
     1861 tggggctcgt gatggggctg aagaatgtca aactgctcgg ccttgttccc catgtctcgg
     1921 gacgggaggg gtatgacccc ggtcggctcg gcatctttaa aaaacggctt gaacaaagcc
     1981 tccgattctc cgctctcgcc caacgggttc atgaggaagg ggatgtccgg cttctggaga
     2041 aagccaacac cttcggatgg gccgcgatcg gaacccaccg ctatcgctat gatctgcgcc
     2101 tcgtccatct gtatgaacgt ttcgacggac gggcggatac ctattccctc atcgaagcgc
     2161 ttgcgttggg ggcgaatctc gaaatctttg aaccttcgcg gattgcccag cttgccagac
     2221 ggtgcaagga gaacggactt gatcccgcat ccgtcggggt tctgttggga tgggcgcggg
     2281 tcgcccggaa agaggggacc ctttcctttc ttcccgatct tcaaagtcgc ctggtcgcca
     2341 actacctcaa agtgctcgat gcgatcagct acggaaaggg gagcggtcat cagcttggaa
     2401 agcgactcga tcaactggtt gcagagtatg gcggaaagaa cgggtgctac tgcatcggca
     2461 gccaacccct cggaccgttt gactatcggg cgcttcccac ccaagcattg ctcgtcagca
     2521 tgggcgatca tcgaatcgtt ctcggagaac tcatccgagg cggacactac cggcgcggca
     2581 aagcgaagcg aagcgctgca tgggctctgt accttcaacg gctctggggc gcccaggagt
     2641 cggtcggcct gtctccgtat gtcgatctgc ccttcttcgg tcgatggtat ccacacatca
     2701 aaggaaggcg ttcgcgcttt gcgatgtccg ctcttattgc ttcgatcagc gaaggaaaaa
     2761 cctttaccgc ccagcacctc gcaagcgtcg gcaagcgggc gtggagcttg gaacgggaaa
     2821 taaaccgtcg attgggctgg caggatcccc ccttcgccga acaacagctt cttgacggcc
     2881 gaagcaactt tcccaagagc caggtcgttc cgcttgccct tcttcgggca gcgtatcacg
     2941 agctcttgaa gcggtggtaa agaaacgggg aatgggtttt caaacgatgg attccaacac
     3001 cctctgtatc aatcagggcg taggtcgcgc cgcttgatga acgcggccgg ctcggggagc
     3061 cggggttgag ccaaagggtt ccatcagcat ctttcttcag atgaggaatg tgggtgtgcc
     3121 cgctgatgac gatccctccg acggtgtacg gaacgtccaa atgaccatgg gtgagggtga
     3181 tcggaagacc ttgatagctc aaggtgcgca caaggggtgg cagatggatt cgagcctgag
     3241 cgaactggta ggagacgtca ctgttgcctc ggacaaggac gaggccgggt aatcttgaaa
     3301 gaagggaggc gaagaccggc tcctcccggg ggcagaggtc gccggcaatc agcacctgct
//
LOCUS       DAOPUZ010000092         3350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_28994_length_3350_cov_3.851897, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000092 DAOPUZ010000000
VERSION     DAOPUZ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28994_length_3350_cov_3.851897"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKN79_02355"
     CDS             <1..591
                     /locus_tag="PKN79_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNZ94634.1"
                     /translation="TRPAIGGNIMATIITPNHRPQMATVRPRSTKILEPDYGRKAVIQ
                     YVEYPDKASVSTISVTGIRPFEGQQSSIDEAEIIVSGGKGLKKKDNVGLIEELAAVLG
                     GEVGATRDAVDRGWISYPHQVGLSGKTVSPKLYLAVGISGAIQHLAGIKTSECIVAIN
                     NDEQANILGLADFAIIGDLFDVVPELTRQLEKRKNA"
     gene            588..2006
                     /locus_tag="PKN79_02360"
     CDS             588..2006
                     /locus_tag="PKN79_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNZ94635.1"
                     /translation="MKHEYGTVTTAIVKQLRKIVGEPNVLVDRERMEGYSHDETSVEQ
                     YAAMPEVVVTPVTTSQVAQIMKLADTHRIPVTPRGAGSGLSGGAIPIYGGIVLSVEKM
                     NSIVEIDEANLTVTAQAGIVTNELNNRLKELGLFFAGYPMSLETCMLGGNIAENAGGG
                     KAIKYGVTGRYILGMEVVTPQGEILNLGGKNTKDVSGYDLKQLYIGSEGTLGVITEAT
                     IKLLGLPKTSSDLLVTFTTPQQAISVVPVLMKQGIIPTSIEFMDYLSTRISCDYLNES
                     LPIEGVGALLLIEIDGIDETVVEQQLIQVGDLCMEQGAIEVYIAEDANNRERVWSVRR
                     AIAEAIKVYSPIQSLEDIVVPIGSIPKVIPFLDKLSKEHDVLIPCYGHAGDGNLHATV
                     IKNPEFTMEHWLEVEPKILGELYEFITSELGGKISGEHGIGLKRREFFKEFTSPIEYR
                     VMEQIKRALDPNGIMNPGKILQ"
     gene            2016..2621
                     /locus_tag="PKN79_02365"
     CDS             2016..2621
                     /locus_tag="PKN79_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ94636.1"
                     /translation="MAEHTQRMTKSDRTKAKIFEIAWNLFKERGFDNVSLQDIADHSG
                     TAKGSFYTYFSTKSDIIVEGFWIIDGYYQTVEKEVLRHQTTEEKLLKFTELQLTYVRD
                     EIGVEMLKVLYANQVLQQDSAKVITNPTRFWHTFIRSIIEKGQINGECRTGLSAEQYA
                     LFFNRSMRGLFLDWNIGSASFDLVEEGLLYCKKFVLPTILL"
     gene            complement(2616..>3350)
                     /locus_tag="PKN79_02370"
     CDS             complement(2616..>3350)
                     /locus_tag="PKN79_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ94637.1"
                     /translation="RHFAPLPPGFVYAHFNDLDSVKALVDERTCAIIVEPIQGEGGIV
                     PATKEFLHGLRDLCDRHELLLIYDEVQCGMGRSGKPFAHQLYGVKPDILTTAKALAGG
                     VPAGAMLTQGEASDVFVPGDHASTFGGNALAARAIIYMCDTLQEKAFLSHVEAMGRYL
                     RMKLEELKVQFPNLITAVRGVGLINGIALTIPPKEVVAKCFEKKLLLASAGSNVVRFV
                     PPLIITEKEIDAAIKILQESLSEVRS"
BASE COUNT          807 a          934 c          956 g          653 t
ORIGIN      
        1 acccgtcccg ccatcggcgg caacatcatg gcgacgatca tcaccccgaa ccaccgcccg
       61 cagatggcga cggtccgccc ccgctccacg aagatcttgg agccggacta tggtcgaaaa
      121 gcggtaatac agtatgttga gtatcccgac aaggcttcag taagcacaat atcagttacc
      181 ggtataagac cctttgaagg gcagcagagc agcattgatg aagcggagat catcgtttcc
      241 ggaggaaagg gcctcaagaa gaaagacaat gtcggattga ttgaagagct tgctgccgtt
      301 ctgggcggcg aggtcggtgc cacccgcgat gccgtcgatc gagggtggat ctcctacccg
      361 caccaagtgg ggctttccgg gaagaccgtc tcgccgaagc tgtacctcgc cgtgggaatc
      421 tccggggcga tccagcacct ggccggtatc aagacctcgg agtgcatcgt cgcgatcaac
      481 aatgatgaac aggcaaatat tctgggactc gccgattttg cgatcatcgg agacctgttc
      541 gacgtggtgc ccgagctcac ccgacagctt gagaaaagga agaacgcatg aagcatgagt
      601 atggaaccgt aacaacagcc atcgtgaagc agctgcggaa gatcgttggc gaacccaacg
      661 tgttggtcga tcgggagcgg atggaaggct acagccacga cgaaacctcc gttgagcaat
      721 atgcggcgat gccggaagta gtcgtgaccc cggtgacgac ctctcaggtg gcgcagatca
      781 tgaagctggc cgacacccat agaatcccgg taacgccccg aggcgccggt tcggggctct
      841 ccggcggggc gatcccgatc tacgggggca tcgttctctc cgtggagaag atgaactcga
      901 tcgtggagat cgacgaagcg aacctcacgg tgaccgccca ggcgggcatc gtcacgaacg
      961 agctcaacaa ccgccttaaa gagctgggac tgttcttcgc cggctatccg atgagcctcg
     1021 agacgtgcat gctcggcgga aacatcgccg agaacgccgg cggggggaaa gcgatcaaat
     1081 acggggttac cggaaggtac atcctcggca tggaagtggt gacgccgcag ggagagatcc
     1141 tgaacctggg cggaaagaac accaaggacg tgagcggcta cgatctgaag cagctgtaca
     1201 tcggctcgga gggaacgctg ggggtcatca ccgaggcgac gatcaagctt ttgggcttgc
     1261 cgaaaacctc ctcggatctg cttgtcacct tcactacccc gcagcaggcg atctccgtcg
     1321 tccccgtatt gatgaagcag ggaatcatcc ccacctccat cgaattcatg gactacctct
     1381 ccaccagaat ctcctgtgac tacctcaatg aaagccttcc gatcgaggga gtcggagcgt
     1441 tgctcctcat cgagatcgac ggcatcgatg aaacggtcgt cgaacaacag ctcatccaag
     1501 tgggcgatct gtgcatggag cagggagcga tcgaggtata tatcgccgaa gatgcgaaca
     1561 accgggaacg ggtctggtcg gtccggcggg cgatcgccga ggcgatcaag gtatacagcc
     1621 cgattcaaag ccttgaagac atcgtcgttc cgatcggctc aatccccaag gtgataccct
     1681 ttttggataa gctctccaaa gaacatgacg tcctgatccc ctgttacggg catgccggag
     1741 acgggaacct ccacgccacg gtgattaaaa accccgaatt cacgatggag cattggcttg
     1801 aggtcgaacc taaaatcctt ggcgaactgt atgagttcat caccagtgaa ctcggtggga
     1861 agatcagcgg cgagcacggg atcggcctca agcgaaggga gttcttcaag gagttcactt
     1921 ctccgatcga gtatcgggtg atggagcaga ttaaacgcgc ccttgacccg aacgggatca
     1981 tgaacccggg aaaaatactg cagtaggagc atggtttggc tgaacataca caacgaatga
     2041 ccaagtccga ccgcacaaag gcgaaaatct tcgaaattgc gtggaacctc ttcaaggaac
     2101 ggggattcga caacgtatcc cttcaggata tcgccgatca ttccggcacg gccaagggaa
     2161 gcttctacac ctacttttcg acaaagagcg acatcatcgt cgaagggttt tggataatcg
     2221 acgggtacta tcaaacggtg gaaaaagagg tcctgcgcca tcaaacaacc gaagagaagc
     2281 ttttgaagtt caccgaactc cagctaacct atgtccgcga tgaaatcggg gtcgaaatgc
     2341 tgaaggttct gtatgccaat caagtgctcc agcaagactc ggcgaaggtc atcaccaatc
     2401 cgacccggtt ctggcatacg ttcatccgct cgatcattga aaagggccaa atcaacggag
     2461 agtgtcgaac cggcctctcg gctgaacaat atgccctgtt cttcaaccgc tcgatgcgcg
     2521 gcctttttct cgattggaac atcggctcgg cctccttcga tctcgtcgaa gaaggccttc
     2581 tctattgcaa aaagttcgtc ctgccgacga tcctgctatg aacgtacctc actcaagctt
     2641 tcttgaagaa tcttgatggc ggcatcaatc tccttctcgg tgatgatgag cggcggaacg
     2701 aagcgaacga cgttcgaacc ggcgcttgca agaaggagct tcttctcaaa gcacttcgcc
     2761 accacttctt tcggagggat cgtgagcgcg atgccgttga tcaggccaac gccgcggacg
     2821 gcggtgatca ggttgggaaa ttgaactttg agctcctcga gcttcattcg aaggtagcgg
     2881 cccatcgctt caacgtggga aagaaatgct ttctcttgaa gagtatcgca catgtagatg
     2941 atcgcccgcg ccgccaaggc gtttccgccg aatgtggaag cgtggtcccc gggaacgaag
     3001 acatcgcttg cttccccttg ggtcagcatc gcccccgccg gaacccctcc ggcaagcgct
     3061 ttggcggtgg tgagaatatc cggcttcacc ccgtagagct gatgggcgaa gggctttccg
     3121 cttcgcccca ttccgcattg cacttcatcg tagatcaaga gcagttcgtg ccggtcgcac
     3181 agatcgcgca ggccgtgaag aaactccttt gtcgccggga cgattccgcc ctcaccctgg
     3241 atcggctcga cgatgatcgc gcacgtccgt tcatcgacga gcgcctttac cgaatcaagg
     3301 tcgttgaaat gcgcatagac gaatccgggg ggaagcggag cgaagtgccg
//
LOCUS       DAOPUZ010000093         3341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_29110_length_3341_cov_3.075167, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000093 DAOPUZ010000000
VERSION     DAOPUZ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29110_length_3341_cov_3.075167"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKN79_02375"
     CDS             complement(<1..874)
                     /locus_tag="PKN79_02375"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNZ94638.1"
                     /translation="MDRYLSVLQREMRVAMGCTEPAAAALCAARASELLGENPVRLHV
                     STSGEMLKNAMGVGIPNTALKGLKAAVALGAAIADIQAGLNILSTIDEAVISKAEGFP
                     VSLTIVKDVPSLYIQVEAVGLNHFARATISGEHERFSELVKDEEVLLSLPLDGCSATL
                     EEVDEVILSKSTLADILSWIEEAPPEAHALVLNALETNLSIARHSLESEYGLTVGKTI
                     FTDCEKELQSLDQAFKRGSALAAAASDARMAGCPLPVVINSGSGNQGITLTVPLAPVA
                     EHLRSSDEELARALL"
     gene            complement(915..2114)
                     /gene="manA"
                     /locus_tag="PKN79_02380"
     CDS             complement(915..2114)
                     /gene="manA"
                     /locus_tag="PKN79_02380"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HNZ94639.1"
                     /translation="MNIVEISGVIKDHTWGNTSSIPSLLGVEEDGKPKAELWFGTHPD
                     GMATVVETGETLQNLLASDTRHWLGEAHLATFGEELPLLLKVLAIGEPLSIQVHPNKK
                     QAEKGWAKEEHLRSRLHKELWNYKDPNRKAEVGYALEPVTALSGFRPLKEIRAWLKRL
                     IPSALQTFFSYLDEEGDEDGHIRRFFEALYTLEKKDLKTVLDELFLSLEDLPLESADR
                     SFLTEKGIVEYCRTFYPLDPGLLAPFFLNVVHLDIGEGLFIPPGVLHAYMKGVVLEVM
                     SNSDNVLRGGLTPKKVDLHELFSIVEVIGKPAELTRQLIGRSGRVHLLTPTEEFHLMV
                     LHSGTYEIDERLSIELLFQTEGETVLKGAGEERRLKKGSCHVVAAGLGSYTLHVEGTL
                     FIADVPR"
     gene            complement(2173..>3341)
                     /locus_tag="PKN79_02385"
     CDS             complement(2173..>3341)
                     /locus_tag="PKN79_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNZ94640.1"
                     /translation="STAAPVCILVPISSWVITVMSIVKSSEGFGVLGVSEFSFFLRSV
                     PYNIYALLTLIMVITTILSGRNYGPMTRSIAYAKETGKLYNEDYGAAPGEIEDVGTDK
                     ADKAKSLDMLFPLIVLILSSVILFPVTTYLGAIGSDGIETYGQAVRSMSLGDAFNNTD
                     ASMALFYAIIFTLSITSVYYLARKLFTLREAGDALTEGIKSMVPALIILTMAWTIGTV
                     ITSSPEDGGLGLASYLSDVVVGGGFPIALVPMIAFVLSALIAFSTGTSWGTFAIMIPI
                     VMPIAVGLAQAKGLDGSALLNAAMISVSAVLGGSVFGDHASPISDTTILSSTGAGCPH
                     LEHVATQMPYALTIAVITAIAFIVGGIFLSVLVAWIVALLLFAGAMYLMPKYFK"
BASE COUNT          793 a          934 c          901 g          713 t
ORIGIN      
        1 ccagcaaagc ccgggcaagc tcttcatcac ttgaacgcaa atgctccgcc actggcgcaa
       61 ggggaacggt caaggtgatc ccctggttgc cgctgccgct gttgatcacc accggaaggg
      121 gacagccggc cattcgagcg tcgcttgccg cggcagccaa cgcggatccc cgcttgaacg
      181 cttgatccaa gctttgcagt tctttctcac agtccgtgaa aatcgttttg cccaccgtca
      241 aaccgtactc gctttcaagc gaatgccggg caatcgagag gttcgtctca agagcgttaa
      301 gcaccagcgc atgagcttca ggcggcgcct cttcaatcca ggagagaata tccgccaagg
      361 tggatttcga aagaatcacc tcgtccacct cttccaaggt agcggagcat ccgtccaaag
      421 gtaatgacag gagcacttcc tcatccttca ccagctcgct gaagcgttcg tgttcgccac
      481 tgattgttgc tcgtgcgaag tggtttaagc caaccgcttc gacttggata tacaaggaag
      541 gcacgtcttt gacgatggtc agggagacgg gaaatccctc cgccttcgaa atgacggcct
      601 catcgatcgt ggagaggatg ttcaacccgg cctggatatc ggcgatcgcc gctccgagcg
      661 ccaccgccgc tttgagacct ttcaacgcgg tgttgggaat ccccaccccc attgcatttt
      721 tcagcatttc tccacttgtt gaaacgtgca gacgtacagg attctcaccg aggagctcgg
      781 aagctctggc agcacagagc gctgcggcgg cgggttcggt acaccccatc gcaacgcgca
      841 tctctctctg caacactgat aaatatctat ccataaaacc gatagtatca cctgacgggc
      901 accgagccaa caccctagcg gggaacatcg gcgataaaca gggttccttc aacatgtaat
      961 gtatagctgc ccaatcccgc tgcgacgacg tggcaactgc ccttcttcaa tctccgctct
     1021 tcgcccgctc ctttgagcac tgtttcgcct tccgtctgga aaagcagttc gatggagagc
     1081 cgttcatcga tctcataggt tcccgagtgg agaaccatca gatgaaactc ttcggtggga
     1141 gtgagaagat ggacccgtcc gctgcgtccg atgagttgcc gagtcaattc agcgggcttt
     1201 ccaatcactt caacgatcga aaacagttca tgaagatcca cttttttggg cgtcaatcct
     1261 cctcgaagaa cgttgtccga attgctcatc acctcaagga cgactccttt catataagcg
     1321 tgcagaactc caggcggaat gaacaatcct tcaccgatat ccaaatgaac cacgttcagg
     1381 aagaacggag ccaacaggcc gggatcgagc ggataaaatg ttcgacagta ttcaacgatt
     1441 cctttctccg tcaggaatga tcgatcagcg ctctcaagcg gaagatcttc caaggaaaga
     1501 aacaactcat ccaagacggt tttcaggtcc tttttttcca aggtgtagag cgcttcaaag
     1561 aagcgacgga tatgtccatc ttcatcacct tcttcatcaa gatacgagaa aaaagtctga
     1621 agagcggaag ggatcaatcg tttgagccat gctcgaatct ccttaagcgg acgaaacccg
     1681 cttaaagcgg taaccggttc aagagcgtag ccgacctctg ctttccggtt gggatctttg
     1741 tagttccaca gttctttatg aaggcgactg cgcaggtgtt cttcctttgc ccatcccttc
     1801 tccgcctgct tcttgttggg gtgaacctga atcgacaggg gctccccgat cgccaagact
     1861 ttcaaaagca gtggaagctc ctcgccgaag gtagccaggt gggcttctcc cagccaatgc
     1921 cgggtgtcgg aagcaagaag gttctgaaga gtttctcccg tttcaactac tgtcgccatt
     1981 ccgtccgggt gggttccaaa ccacaattca gctttgggtt tgccatcctc ctcaactccg
     2041 agcagggaag gaatcgatga ggtattcccc catgtatggt cttttatcac accgctgatc
     2101 tcaacaatat tcatggctcc tccacactgt gagtatagag gagccatgag caaagagaaa
     2161 gctttgagag gattatttga aatatttcgg catgagatac atcgccccgg caaagagcag
     2221 gagcgcgacg atccaagcga caagaacgct taggaagatt ccgccgacga tgaaggcgat
     2281 cgcggttatg acggcgatcg tcaacgcata gggcatctgg gtggccacgt gctcaagatg
     2341 cggacatccc gctccggtgg aggaaaggat cgtcgtgtca ctgatcgggc tggcgtggtc
     2401 accgaagacg gaacctccaa gaaccgcgct gacgctgatc atcgccgcgt tcagcaaggc
     2461 cgaaccgtcg agtcccttcg cctgggcgag accgacggcg atcggcatca cgatcggaat
     2521 catgatcgcg aacgttcccc agctggtccc ggtcgagaaa gcgatcagag cggagaggac
     2581 gaaggcgatc atcgggacaa gagcgatcgg gaagccgccg cctacgacca catcgctgag
     2641 gtaggaggcc aacccgagtc cgccatcctc cggcgaggag gtgatgaccg tcccgatcgt
     2701 ccatgccatc gtcaggatga tgagggcggg aaccatcgat ttgattcctt cggtcagggc
     2761 gtctcccgct tcgcggagcg tgaagagctt tcgggcgagg tagtagaccg atgtgatcga
     2821 caacgtgaag atgattgcat agaagagggc catcgatgcg tcggtgttgt tgaacgcatc
     2881 accgaggctc atcgatcgaa ccgcctggcc gtacgtctcg attccgtcgc ttccgatcgc
     2941 cccgaggtag gtcgtgacag gaaagaggat gacgctggag aggatcagca cgatgagcgg
     3001 aaagagcata tcaagactct tcgccttatc cgccttgtcg gtgccgacat cttcaatctc
     3061 accgggggcc gctccatagt cctcgttgta cagctttccg gtttccttcg cataggcgat
     3121 cgagcgcgtc atcggtccgt agttccgacc gctgagaatc gtggtgatca ccatgatcag
     3181 cgtaaggagc gcatagatat tgtagggaac ggagcgaagg aagaaggaga actcactgac
     3241 ccccaatact ccaaagcctt cggaggattt cacgatcgac atgacggtga tgacccatga
     3301 ggagatcgga accagaatgc acaccggagc ggcggtcgag t
//
LOCUS       DAOPUZ010000094         3335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_29181_length_3335_cov_3.784146, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000094 DAOPUZ010000000
VERSION     DAOPUZ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29181_length_3335_cov_3.784146"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKN79_02390"
     CDS             <1..453
                     /locus_tag="PKN79_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94641.1"
                     /translation="LKGTIDSKELTISVRGEGMDEELNEYDVLMFDFPITDHDKRSWV
                     KEVPLHFTYSSNKNLPSKAHLEFVVGEFVGDSGFKLKPELKIARPSNKIDQDSDPRRI
                     LLETTFESGWQEDTPIVTVIVVFTKAEEELFPVGNYTGVFSINYLGIN"
     gene            complement(454..1872)
                     /locus_tag="PKN79_02395"
     CDS             complement(454..1872)
                     /locus_tag="PKN79_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94642.1"
                     /translation="MYLNKLFERSTGRTLLLFQETKRVKGKNRTRTVERIGYLDELEK
                     IYDDPIAHFKEEAKRRSVRQKLNVEVSLDEHHQFDAESREVKAPGLSLGVLPLSQIYH
                     ELEIGSFWDNRRRYTKARYNHNAIFQALVFGKILFPESQLSTWRNRLGLLSNMDFTDE
                     DVRHALPFFEAHKTALQKHLHKKVGTLYGRDPSVLYRARVPNGRAGEEHHIQMGLLTD
                     GNGLPIAYGLFGNDGSTLARMMKEVDEQLEIQKVVHVSDKGMIDAKDKEEILANKGGY
                     IFGTPLSKTDADAKAYIPDETGYKHPNASERMTVNERQIVFREEDGYHVIHTNVIGSE
                     DESSSLFRLERPATALDIIDMYRGLWRIKERFLGSNQEKESIEAHLLSSFVSLLILKI
                     LELKTGKTIEFEKMVDDLRRLQVVEETDDVYLLLEPTNIIKAIGDAMDLDLVKVRYTA
                     RELKNLVAKTRKQGMVPDSARG"
     gene            complement(2055..3083)
                     /locus_tag="PKN79_02400"
     CDS             complement(2055..3083)
                     /locus_tag="PKN79_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNZ94643.1"
                     /translation="MARTINRLRVLNLIAREGAISRAEIARVLDLSKPSTSEIVALLL
                     EENLVMESGKAETASGRKPTNLKLKSDGALVLGIDMDVRTTTYTVANIHGAILRMEKF
                     PIQEHPDPKTAGAEVIKRVLKLTRESKVPFKAIVLTTGGTFSEDRRTLVAHNYWSWEQ
                     IPLAEVIEKNTSIPTIFAHTTRSMSFAERWFHKETPSSYLYINWGEHLESALYINNVD
                     RLSRFEHTPVGQTGLCSCGQIGCLETIAGLSRIGKEDLPHAAMAIGKALIAADRATAV
                     DAIIIAGPQAREYLPIIQENYQGSTPIIPSILKERSTPLASVAVALDWGIFERSVLEQ
                     AKTATRNG"
BASE COUNT          722 a          872 c          864 g          877 t
ORIGIN      
        1 ctgaaaggga caatcgactc taaggagttg acgatctcgg tgagagggga aggaatggat
       61 gaggagctca atgaatacga tgtcttgatg tttgactttc ccatcaccga tcatgataaa
      121 agatcgtggg tgaaggaagt tcctctgcac tttacctact catccaataa gaatctgccg
      181 agtaaagcac atttggaatt cgtcgttggc gagtttgtcg gagacagcgg cttcaaactg
      241 aaacccgagc tgaaaatcgc cagaccaagc aacaagatcg atcaggattc cgatcccaga
      301 cggattctac ttgaaacgac cttcgagtcc ggttggcagg aagatacacc gattgtgacc
      361 gtgatcgtcg tctttaccaa ggctgaagag gaattattcc cggtcggaaa ctataccggc
      421 gtgttttcca tcaattatct gggaataaac tgattatcct cttgcgctgt ccgggaccat
      481 tccctgtttt cgtgtttttg ccaccagatt cttcagttcc cgggctgtat atcgtacttt
      541 cactaagtct aaatccatcg catcaccaat ggccttgatg atgttcgtcg gttccagaag
      601 caggtagaca tcatccgtct cctccaccac ctgcaatctc cgtagatcat cgaccatctt
      661 ttcgaactcg atggtcttgc ctgtcttcag ctccagaatt ttcagaatga gcaagctcac
      721 gaaactgctt agcaggtggg cttcgatgga ttctttctct tggttgcttc cgagaaagcg
      781 ttccttgatt cgccataggc ccctatacat atcaatgatg tccaaggctg tcgcaggcct
      841 ctcgagccgg aagagagaag aggactcatc ttccgagccg atgacattgg tgtggatcac
      901 atggtatccg tcctcctccc ggaagacgat ctgacgctca ttgaccgtca tccgctccga
      961 agcgtttgga tgtttgtatc ccgtctcatc cggaatatac gccttggcat cggcatcggt
     1021 cttcgatagg ggggtgccga agatgtaccc tcccttgttg gccaatatct cctctttatc
     1081 cttggcgtcg atcatccctt tgtctgaaac atggacaacc ttttggattt ccaattgttc
     1141 atcgacctct ttcatcatcc gtgcgagggt cgatccatca ttaccgaaaa gtccataggc
     1201 gatcggcaac ccattgccgt ccgtcaacag acccatttgg atgtgatgct cttcgcctgc
     1261 tcttccgttc ggcaccctcg ctctatacag caccgatgga tctcgtccgt acagcgtgcc
     1321 gaccttctta tgcagatgct tctgcaatgc cgtcttatgg gcttcgaaaa agggaagggc
     1381 gtgccgtaca tcctcatcgg tgaaatccat gttcgagagc agtcctagac ggtttcgcca
     1441 ggtgcttaat tgcgattccg gaaagaggat ttttccaaag accagagctt ggaagattgc
     1501 attgtggttg taccgggctt tcgtataccg ccttcggtta tcccagaaag acccgatctc
     1561 caattcatga tagatctgcg agaggggaag cacccccagt gaaagaccgg gggctttcac
     1621 ctctctgctt tccgcatcga attgatggtg ttcatccagg gacacttcaa cgttgagctt
     1681 ttggcgaaca cttcttcgtt tcgcctcttc tttgaaatga gcaatcggat catcatagat
     1741 cttttccagt tcatccagat agccgattcg ctcgactgtt ctggtccggt tctttccttt
     1801 gacccgcttg gtttcctgaa acagtaataa tgttcgtccg gtgctgcgct cgaaaagctt
     1861 attcaggtac atggcaccag tgtatcacac ctgatatccg gggaaatacg gataatcggc
     1921 aatatttgtt ttacagctta cagaatccat gcaataataa aggtgtcgtt gctcggacct
     1981 ggtcgatttt tccagcggca ggtctgatac ctcaagcttg gaggagtatt gaggttctct
     2041 caaggaggtt accgctatcc gtttcgggta gcggtttttg cttgttcaag caccgaccgt
     2101 tcaaagattc cccagtccag cgccacggcg acggaagcaa gcggcgtgct ccgttctttg
     2161 aggatgcttg ggatgatcgg ggtcgagccc tgataattct cttgaatgat cggaaggtat
     2221 tctcttgcct gagggccggc gatgatgatc gcatccaccg ccgtcgcccg atccgccgcg
     2281 atgagggcct ttccgatcgc catggcggca tggggcagat cttcctttcc gatcctcgag
     2341 agaccggcga tcgtttccag acacccgatc tgtccgcagg agcagagccc cgtctgaccg
     2401 accggggtgt gttcgaagcg ggagaggcgg tcgacgttgt tgatgtacag agcgctctcc
     2461 aagtgttcgc cccaattgat gtagagatag gaagagggag tctccttgtg gaaccaacgc
     2521 tcggcgaagc tcatcgaacg ggtcgtatgg gcgaagatcg tcggaatcga ggtgttcttt
     2581 tcgatcacct cggcaagggg gatctgctcc catgaccagt agttgtgggc aacgagagtc
     2641 cttcgatcct cgctgaacgt tccgccggtg gtgaggacga tcgccttgaa gggaactttg
     2701 ctttcgcggg tgagcttgag gacccgcttg atgacctccg ctccggccgt cttcggatcg
     2761 gggtgttcct gaatcgggaa cttttccatt ctgaggatcg caccgtggat gttggcgacc
     2821 gtgtatgtgg tcgtcctgac atccatgtcg atgccgagaa ccagcgctcc gtcactcttg
     2881 agctttagat tggtcggctt ccggccggaa gcggtctcgg ctttgccgct ctccatgacg
     2941 aggttctctt cgagaagaag cgcgacgatc tcgcttgtcg agggcttgct cagatccaac
     3001 actcgggcga tctccgcccg gctgatcgct ccttcccggg cgataaggtt gagcactcgg
     3061 aggcggttga tggttctggc cacggaaggg tgggataggt ccatgcccca gcatacccca
     3121 ccgacgaccg ttttgttaag gtttattgcc gattggaaca aatctcagta tcttttgata
     3181 tattcccttg gaggtatgaa catataatgg caaagaaaaa gttcaagacg gaagtgaccg
     3241 agctgatgca gctcattatt cactccctgt attcgcacaa agagattttc ctgcgggaac
     3301 tgatctccaa tgcctcggac gccatcgaca aaatc
//
LOCUS       DAOPUZ010000095         3323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_29335_length_3323_cov_3.437576, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000095 DAOPUZ010000000
VERSION     DAOPUZ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29335_length_3323_cov_3.437576"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2035
                     /locus_tag="PKN79_02405"
     CDS             <1..2035
                     /locus_tag="PKN79_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNZ94644.1"
                     /translation="VLDALSESPYLASRFSLFDFVTLEKRGTRLMSVPFASSSWTDEE
                     AVLLKERVQSDPMVKNYLVSEDLHGLFINFESVDLTPQMETELREILQPLVTQGMSVS
                     INGAAILTNVLMRYLSRDLVLILTLCLLAILVVYYLSFRAKRSVLLPFSMSAMGIIWT
                     FGTMRLLGYSLTIVNIITPAMVLNLGSSYAIHVISEYFAGHEIGRGVIASTRKILRTI
                     LFACLTTVIGFLSLLTSKTGALKEFGISVAIGITSCAILAATYLPAVLSLVRKPKVHH
                     IKTYRRGYLASIVNGINVLVGRYRMVFVVLWLVIIAGFGLTRDKISVNTNYMSYLPKR
                     DPFTISSRHFARTMSGNTPYYIIIDAPSGTTNFFLEPENLARVYAFEEKVKKENNDIR
                     QSLSFASYVAFANEVYSNERSIPSSKGLLNLLSRMVLLLSKQGKQDLESVISSDGNTL
                     TIILQNYDAVEEEFGTIGSAERIEDTILDNLQLLPGGTIVTLDGEPHQALHFSNTLLH
                     DQQMSIYFSFIFVFLVVLIAFRSFSLACYALIPVVTGVMANYIIMFLLNIPFDLITVS
                     FGAVSVGVGVDYAIHFLLRYRNKIDPTQNRDVALLLKETISETGRPIILTTLSIVAGM
                     LMFLFASYAPVRIFGILMSTALLNCMLATLFIMPSVIWAVSVLKERGLRRRLS"
     gene            2032..3150
                     /locus_tag="PKN79_02410"
     CDS             2032..3150
                     /locus_tag="PKN79_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ94645.1"
                     /translation="MTISLLYVDAGKGHYVPAKAMGDVLAARGYDAPVEDLFVALDST
                     FWHWFCKAEWRFFLKHPILERIVHGVSDNRFSNVILRALSKAKPIRTKFIAWYKERKP
                     DVILSTNFLGGVIVGELAAYYKLDVKVYVYAADAFNNPVAGFHINVDKIFIPSFVGEE
                     NLERQGYSQDQIIRSPFPLQQSIEKMERVSKQEARSILGLEDRFTILYNLGGEGIGSD
                     KLIRHLVKEEADYQIVIVGKTDELTKKHFLEITKDSNLRVHVPGFVTNIGMYMLACDI
                     QAGKAGANALMESIALRRPFLLSQVLYAARDTTRFFKLRGVGWVEGSSKKQAEIIKTY
                     ARSEEVRAEMDSHFDKLPLTFSAEAFVDLLLKSVNQSR"
     gene            complement(3125..>3323)
                     /locus_tag="PKN79_02415"
     CDS             complement(3125..>3323)
                     /locus_tag="PKN79_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94646.1"
                     /translation="GTGQTGYRVVSTIGLDGWVILNRYPAYPIRASLGLNLEDLRRAV
                     ADELSWRDVEWELFIGFDLHF"
BASE COUNT          762 a          933 c          807 g          821 t
ORIGIN      
        1 agtgctcgac gctctctccg agtcccccta tctcgcctcc cggttcagcc tctttgactt
       61 cgtcaccctt gagaagcggg gaacccgcct gatgagcgtc ccttttgcat cctcgtcatg
      121 gaccgatgaa gaagcagtcc tgctcaagga acgggtgcaa agcgatccga tggtgaagaa
      181 ctacctcgtc agcgaggatt tgcacggctt gttcataaac tttgaatcgg ttgacctcac
      241 cccgcagatg gagacggagc tcagggagat cctgcaaccg cttgtcacgc agggaatgag
      301 cgtttcgatc aacggagcag cgatcctgac caatgttctg atgcgctacc tctcccgaga
      361 tttggttctc atcctcaccc tgtgtctctt ggcgattctt gtcgtgtact acctctcatt
      421 ccgagccaaa cgcagtgtcc tcctgccctt ttcaatgtcg gcgatgggga tcatttggac
      481 ctttggaacg atgaggctgc tcggctacag tctgacgatc gtcaatatca tcactccggc
      541 gatggtcctc aaccttggat cctcctacgc aattcacgta atcagcgagt attttgccgg
      601 acatgagatc ggtcggggag tgattgcatc gacccggaag atccttcgaa cgattctctt
      661 tgcctgcctg accaccgtca tagggttttt aagcctgctt acctccaaaa cgggagcgtt
      721 gaaggagttt ggaatctctg tcgcgatcgg aatcacctcc tgcgcgatcc tcgcagcaac
      781 ctatcttccg gccgtcctga gccttgtccg caaacccaag gtccaccata tcaaaacgta
      841 ccgaagaggc tacctcgctt cgatcgtcaa cggcataaac gttttggtgg gacgctaccg
      901 gatggttttt gtggtgctgt ggcttgtgat catcgccgga tttggtctca cccgtgataa
      961 aatctcggtg aacaccaact atatgtccta tctgcccaaa cgggatccct tcacgatcag
     1021 ctcccgtcac ttcgcccgca cgatgagtgg aaacaccccc tactatatca tcattgatgc
     1081 gccgagcggg acgaccaact ttttcctgga acctgagaat cttgcgcggg tctatgcgtt
     1141 tgaagaaaag gtcaagaaag aaaacaacga tatccgtcaa agtctttcct tcgcttccta
     1201 cgtcgcgttc gccaatgaag tgtactccaa cgaacgttca attccctctt cgaaaggatt
     1261 gctgaacctt ctttcacgta tggtgctcct gttgagcaaa caaggcaaac aggatcttga
     1321 atccgtgatc agcagcgacg ggaacaccct cacgatcatt cttcaaaact atgacgccgt
     1381 cgaagaagag ttcggaacga tcggcagcgc cgagcgaatt gaagatacga ttctcgacaa
     1441 cctccaactg ctccccggtg gcacgatcgt aaccttggac ggcgaacccc accaggcgct
     1501 ccatttctcc aacaccctcc ttcatgatca gcagatgtcg atctatttca gcttcatctt
     1561 tgtcttcctc gtcgttctca tcgctttcag gtccttctcc cttgcctgtt acgcccttat
     1621 tcccgttgtc accggagtga tggcgaacta catcatcatg tttctgctca acattccctt
     1681 cgatctgatc accgtgagct tcggagcggt ttccgtcgga gtcggagtcg attatgcgat
     1741 tcatttcctc ttgcgctatc gaaacaagat cgacccgacc caaaaccgag atgtcgccct
     1801 cctgctgaag gagacgatca gtgaaaccgg aaggcccatc attctcacga ccctttcaat
     1861 cgttgccggg atgctgatgt ttctctttgc atcctatgct ccggttcgaa tttttggtat
     1921 ccttatgtcg actgcattgt tgaactgtat gctcgccacc ctcttcatca tgccctccgt
     1981 gatttgggcg gtcagcgtcc taaaagaaag gggacttcgt aggagacttt catgacgatc
     2041 tcactcttgt atgtcgatgc aggcaaggga cattatgttc cggccaaagc gatgggcgat
     2101 gtacttgccg ctcgaggcta cgatgccccg gttgaagacc tttttgtcgc tcttgattcg
     2161 acattctggc attggttttg taaagcggag tggcgcttct ttctgaagca tcccattctg
     2221 gaacggatcg ttcacggcgt atcggacaat cgattctcca acgtcattct tcgggcgctc
     2281 tccaaagcaa aaccgatccg aaccaaattc atcgcttggt acaaggaacg caaacccgac
     2341 gtgatcctgt cgacgaactt cctcggcggg gtgatagtcg gggagctggc cgcctactac
     2401 aagcttgacg tgaaggtata tgtctacgca gcggatgcgt tcaacaaccc tgttgccggc
     2461 tttcacatca atgtggacaa gatctttatc ccttctttcg tcggagaaga aaatttggag
     2521 agacagggat actcccaaga tcagatcatt cgttcgcctt tccccctgca gcaatccatc
     2581 gagaagatgg aacgggtatc caaacaagag gcgcgatcga ttcttggctt ggaagaccgg
     2641 ttcaccattc tgtacaacct cggcggagaa ggaataggat cggacaaatt aatcagacac
     2701 ctcgtaaaag aggaagcgga ttaccagatc gtcatcgtcg gcaaaacgga tgagctcacg
     2761 aagaaacact ttcttgaaat cacgaaagac tccaaccttc gagtgcatgt tccgggtttt
     2821 gtcaccaaca tcgggatgta tatgctcgcc tgcgacattc aggcgggaaa agcgggagcg
     2881 aacgcgctga tggaatcgat cgccctcaga agaccctttc ttctctctca ggttctctat
     2941 gcagcgcgcg ataccacccg gttcttcaag ctcagaggtg ttggttgggt cgagggctct
     3001 tcgaagaaac aagctgaaat aatcaagacg tacgcacgat cggaagaggt ccgagcggag
     3061 atggattccc attttgacaa gcttccgcta accttcagcg ccgaggcgtt cgtcgatctt
     3121 ctgcttaaaa gtgtaaatca aagccgatga agagctccca ctccacgtct ctccaggaca
     3181 gctcgtcggc gaccgcccgc cgcagatcct caaggttcaa gcccaatgaa gcacggatcg
     3241 gataggcggg atagcggttg agaatgaccc agccgtcaag accgatcgtg cttacaacgc
     3301 gatacccggt ttgtcctgtg ccg
//
LOCUS       DAOPUZ010000096         3294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_29669_length_3294_cov_2.895029, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000096 DAOPUZ010000000
VERSION     DAOPUZ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29669_length_3294_cov_2.895029"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="PKN79_02420"
     CDS             complement(<1..1569)
                     /locus_tag="PKN79_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNZ94647.1"
                     /translation="MTRSKGRAHSVTPGWILKVFIPIASIFLLTMIIMALLFSGMFTN
                     VAQSLIVDDFLASLKMISTYYRQMRFNTVPILDDLSDAQEIRDYLLQNLPKDKAMLGA
                     YLRLENTVARNSYIHSIYLYNDEYGFFSTISGAEIPGELSDSTLLEFLATRPKNKNLY
                     QRRSVFTDKTIPLSLPKKPNTPTNLYTICNLSYGDDGEVQSGIIMNLSETLARSLLAD
                     DEGTSKNFYMIDEGSYIISHPDPDQFGRKATSLPLLSQIASFDGPKGGQTLKDDEGKQ
                     FLACWYDIGEMNWRLIFILPMSHIREPLNHLRKNLVFVFIGLALIASLVLVWESKRIN
                     NQLTRENRFIDYLTGGLSAEALPLYEGQTFFIAVFLRQTKKPESELLQKNRFISFASQ
                     YLRFNGKNSFLLTMDQGQHVYLALHKPEKVISRFAKLLIDLGDLFDEQLSIFYSTERV
                     PLEDLPRRYEQMRRRLLSSSLTEVGFISEIEQEDTKEVNLFLTETADMEKALSQRSAE
                     AYAKSVDTLIINLKR"
     gene            1751..3052
                     /locus_tag="PKN79_02425"
     CDS             1751..3052
                     /locus_tag="PKN79_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ94648.1"
                     /translation="MRTKKILLVLTLIACLASSVFAAGASETKAAPAADGPVVISYYT
                     WDDSSHKALIDTFNATHKDIQVKAQILPAADYETKLTTLLSGRADIDAFMEKRQVDMF
                     GQYDNGYLEPLDSYIAATGKPNPAVEAFKSSSVINGDTVVIPWRGGAYYTYYNKKVFE
                     KAGVPTPDYYVKRGEWTWKKFEEVAKAITAADPSLVGSTIYIWGGQGFFQAGQAGDSI
                     VTPDGKIDNIDNVVKQLEMRKRLEDVGAMWSLVDMKVTKLHYSKQFYDGNVGMLLIGE
                     WFPGQMVTGRNDGLLAYDFEDWGITRLPCDQSEYVTVGLPTGNCITSYSKKKQAAFEF
                     INWMSGPEGAAIAASYGVLPAAPGEAAKAEIAKNLPKGNSLDYFLEEKGNNTANFSPY
                     GTRVETEFAKLQEDFLLGNINIEQFKTNFTKLLNDIIKTTY"
BASE COUNT          804 a          869 c          898 g          723 t
ORIGIN      
        1 ccttttgagg ttgatgatca aggtgtcaac cgacttggcg tacgcttccg ccgagcgctg
       61 ggacaacgcc ttctccatgt ccgccgtctc ggtgaggaac aggtttacct ccttggtgtc
      121 ctcctgttcg atttcgctga tgaagccgac ttccgtcagg gagctggaca ggagccggcg
      181 cctcatctgc tcataccgcc ggggaagatc ttcaagcggc acccgttccg ttgaatagaa
      241 gatggaaagc tgttcatcga acagatctcc aaggtcgatg agcagttttg cgaaccggga
      301 gatcactttc tccggtttat gcaatgccag atacacatgc tggccctgat ccatcgtcag
      361 caaaaacgag ttcttcccgt tgaatcgaag atattgggaa gcgaaggaaa taaaccggtt
      421 cttctgaaga agctccgatt ccggcttctt tgtctgtcgg aggaacaccg cgatgaagaa
      481 cgtttggccc tcatagagcg gcaaggcctc ggcgctgagt cctccggtga ggtagtcgat
      541 gaatcgattc tcccgcgtca gctgattgtt gatcctcttg gattcccaca ccagcaccag
      601 tgaggcgatg agcgccaggc cgatgaagac aaacacgagg ttttttctta aatggttcag
      661 cggctcccga atatgggaca tgggaaggat gaagatcagc cgccagttca tctcgccgat
      721 gtcataccag catgcaagga attgcttgcc ctcgtcatcc ttgagcgttt ggcctccttt
      781 cggcccgtcg aacgatgcga tctgggagag cagcggcaac gaagtcgctt tacgaccgaa
      841 ctgatcggga tcgggatggc tgatgatgta agagccttca tcgatcatgt agaagttctt
      901 tgaggtgccc tcgtcatcgg cgaggaggct ccgggcaagc gtctcggaaa ggttcatgat
      961 gatccccgac tgcacttccc cgtcatctcc gtagctgagg ttgcagatcg tgtagagatt
     1021 ggtgggggtg ttcggctttt tgggaaggga caggggaatt gtcttgtcag tgaacaccga
     1081 ccgccgctgg tagaggtttt tgttcttcgg acgggtggcc agaaactcga gcagcgtgga
     1141 atcgctcaat tccccgggaa tctccgcccc gctgatcgtg gagaaaaagc cgtattcatc
     1201 gttgtacaga tagatcgaat ggatatagga gttccgcgcc acggtgtttt ccaaccggag
     1261 atacgcaccg agcatcgcct tgtccttggg aagattctgt aataaatagt cacggatctc
     1321 ctgagcatcg ctcaggtcgt ccagaatggg aaccgtgttg aaccgcatct gccggtaata
     1381 ggtgctgatc atcttcagcg aagcgaggaa gtcgtcgacg atcaacgatt gagccacgtt
     1441 ggtgaacatc ccgctgaaca gcaacgccat aatgatcatg gtaagcagga agatggaagc
     1501 gatcggaatg aaaaccttca gaatccagcc gggagtcact gaatgtgccc gaccttttga
     1561 tctggtcatg gtactcctcc tcatggatac caatacacca tcaagttgca caaatcaata
     1621 cattattggt ttttcaaaac cagaatcttc gcttttcaaa ataaatcggc tctttcaata
     1681 ttgcaatcaa aactcaccca tgcaaggctg aagtatcaac tcacatgttc agagtgaaag
     1741 gaggaacatt atgagaacaa agaaaatact gcttgtctta acgctcattg cgtgcttggc
     1801 aagttcggta ttcgccgcag gggcgtccga aacgaaggcg gcacccgctg ctgacggccc
     1861 tgtcgttatc tcctattata cgtgggatga cagcagccac aaggcgctta tcgacacctt
     1921 caacgccacg cacaaggata ttcaggtaaa agcgcagatt cttcccgcag cggactacga
     1981 aacgaaattg accacgctgc tcagcggccg cgccgatatc gatgcgttta tggaaaagcg
     2041 ccaggtagac atgttcgggc agtacgacaa cggatatctg gaaccccttg attcctacat
     2101 cgccgctacc ggcaagccca acccggccgt cgaagcgttc aagtcgagct ccgtcatcaa
     2161 cggtgacacg gtcgtgattc catggagagg cggtgcgtac tacacctact acaacaagaa
     2221 ggtcttcgag aaggccggag tccccacgcc ggattactat gtgaagcgcg gcgaatggac
     2281 ctggaaaaag ttcgaggaag tcgccaaggc gatcaccgcc gccgacccga gcctggtcgg
     2341 ttccacgatc tacatctggg gcggacaggg cttcttccag gccggacagg ccggggactc
     2401 gatcgtcacg ccggacggca agatcgacaa catcgacaac gtcgtcaagc agctggagat
     2461 gagaaagcgg ctggaagatg tcggggcgat gtggagcctc gtggacatga aagtcacgaa
     2521 actgcactat tccaagcagt tctatgatgg aaacgtcgga atgctcctga tcggcgagtg
     2581 gttccccggg cagatggtaa ccggacggaa cgacggattg ctcgcctatg actttgagga
     2641 ctggggcatc accagactcc cgtgcgacca gagcgaatat gtaacggtcg gacttcccac
     2701 cggaaactgc atcacttcat actcgaagaa gaagcaggcc gcgttcgagt tcatcaactg
     2761 gatgagcgga cccgagggcg cggcgatcgc ggcgtcgtac ggagttcttc ccgcagcgcc
     2821 gggtgaagcc gccaaggccg aaatcgccaa gaatcttccg aaggggaatt ccctcgacta
     2881 cttccttgag gagaagggga acaataccgc caacttcagt ccctatggaa ccagagtcga
     2941 aacggagttt gcgaagcttc aggaggactt cctcttgggc aacatcaaca tcgaacagtt
     3001 caagacgaac tttacgaagc tcttgaacga catcatcaag acgacctact gatcgtcgcg
     3061 acgagaccta tgacgctctc ttccatggaa cctccgtgga agagagccct cttggaggaa
     3121 ctgcgtgaac atgtcacatt ctttgaagag aaccggaaca attctttcgt tcctgcttcc
     3181 cagcttcatt ggattcatca tcttcgtcct gctgccgatg atcatggcgt tcggactttc
     3241 gttcaccaac tattcaggcg gcccgaactt ccggttcatc gggatgaaga acta
//
LOCUS       DAOPUZ010000097         3287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_29757_length_3287_cov_3.157178, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000097 DAOPUZ010000000
VERSION     DAOPUZ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29757_length_3287_cov_3.157178"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PKN79_02430"
     CDS             complement(<1..927)
                     /locus_tag="PKN79_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94649.1"
                     /translation="MSERRIRTMLQGVGINPSSLQRDDQSQAFYREVVASRLNIFTSY
                     GSGDEEYLQTLDEQIRVFDEIYVDTAPIIQKDWFLHFVADSKDILKRRKKRLIILEKT
                     MEELYGLKSNPEKDQDVRIRAIIRPDLIRSLAKQRIVRLGDTGSGGIADDHLVELFRR
                     IGANLNLMLITQDRGLSERIVHLATELGEECEEVKPLTFLEKLIGKKEREVITHRMIA
                     CKLIEGGKLKRLYICPECSQSYYDDLYATEGIVLCGRCYIRLREEEERKELANRKRRE
                     AQLKAEEERQKKLEAEEERLKRERNRLTVEKLI"
     gene            complement(994..>3287)
                     /gene="topA"
                     /locus_tag="PKN79_02435"
     CDS             complement(994..>3287)
                     /gene="topA"
                     /locus_tag="PKN79_02435"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNZ94650.1"
                     /translation="DRLLLATDEDREGESISWHLLEVLKPKIPYRRMVFHEITKKAIT
                     NALETGRDLDENLVHAQEGRRLVDRLYGYTLSPTLWKKLSNKKLSAGRVQSVGLRLTV
                     DRERMRIAFEQNTYWDASAELTNAKGELFEAKLVSYQDRAIASSRDFDATTGAYKGKK
                     GTLHLGEEEARAIVKELKEEQFVVTAIERKPFITRPSIPFTTSTLQQDATRKLRISAS
                     EVMRIAQRLYESGYITYMRTDSPSLSREGTMAARTQVEELYGTDYLSPKARYFAAKSA
                     QAQEAHEAIRPAGDAFVHPKDTRLSGRELALYDLIWKRTLACQMADAQKATTTATIEA
                     GAGIFTATGTEIVFPGFIRAYVEEVEDNEIDKDKILPPLSEGEVCPLKSLTAVEHQTR
                     LPARYTEASLVQELEKRGIGRPSTYATIIKTLLDRKYVVKEGSALVPTFIGFGVCQFL
                     ETNFKQFIDYDFTVRMEEDLDKIARGELKKETFLAEFFLGEHGLMEQNRQQLTLDNKG
                     ESKTLNLCHIDEANPIMIGPYGPYVVLKDGQLVSLPYGWTPGSITDADVKALIANGKQ
                     QPEAKTLGPGPNTGEPIQLLSGRFGPYWQEGEGKDAKRASVPKWVQDGDLQEDGAIAT
                     RYLSLPRILGKDKEGMEVVATKGKYGPYLASGDKTRKLNRLDKDKQLFSITLEEALEL
                     LGKEEVKGSKKRGVRTASAPLKELGEFEGRKITLHNGRYGFYLKHGTSNVTLPNEYKK
                     DEEKALSLRPEEAQRIVEEKQST"
BASE COUNT          603 a          957 c          869 g          858 t
ORIGIN      
        1 gatgagcttt tcaacggtaa ggcggtttcg ttcccgttta agccgctctt cctccgcttc
       61 cagcttcttc tggcgttcct cctccgcttt gagctgagct tcccgccgct tccggtttgc
      121 cagttccttg cgctcctctt cctcgcgaag ccgtatgtag caccgcccgc agaggacgat
      181 cccctcggtg gcgtagagat catcgtaata gctttggctg cactccggac agatgtacag
      241 ccgcttcagc ttcccccctt cgatcagctt gcatgcgatc atccgatggg tgattacctc
      301 gcgttctttt ttaccgatca gcttttcgag gaaggtaagc ggtttcactt cttcacattc
      361 ctccccaagc tcggtggcca gatgaacgat ccgctcgctc aagccccgat cctgggtgat
      421 caacatgaga ttgagattgg cgccgattct tcgaaagagt tccaccagat ggtcgtcggc
      481 gattccaccg ctgccggtgt cgccgagccg aacgattcgc tgcttcgcca aggaacggat
      541 caggtccggg cggattatcg cccggatgcg gacatcctga tccttttcgg ggttgctctt
      601 cagtccatag agctcttcca tcgtcttttc aaggatgata agccgttttt tgcggcgttt
      661 gagaatatcc ttgctgtctg cgacgaagtg aaggaaccaa tccttctgaa tgatcggggc
      721 ggtatcgacg tagatctcat caaacacccg gatctgctcg tcgagggttt gaagatactc
      781 ctcatcaccg cttccgtagg aagtgaatat attgagtctg ctcgcgacca cctcgcgata
      841 gaacgcttgg ctttggtcgt cgcgctgcag agaggaagga ttgattccca ctccttgcaa
      901 catcgtgcga attcgtcgct cgctcaccct gaattcgaga gccaaagccg atatcgctga
      961 tggttttttg gttcgttggt gcgtttgcat cgtttaggtg gattgctttt cctccacaat
     1021 ccgctgcgct tcctcaggac ggagactcag cgctttctcc tcatcttttt tatattcatt
     1081 gggcagagtg acattgctcg taccgtgctt caggtagaag ccatatcgtc cattgtggag
     1141 ggtgatcttc cgaccttcga actcaccgag ctctttcaaa ggcgcggaag cggtgcgaac
     1201 tcctcgtttc ttcgacccct ttacctcctc ttttccaaga agctcaaggg cttcctccaa
     1261 ggtgatgctg aagagctgct tgtctttgtc caaacggttg agcttacgtg tcttgtcgcc
     1321 actggcgaga tacggaccgt acttgccctt cgttgcaacc acttccatcc cctctttgtc
     1381 tttgccgagg attctcggca agctcaaata gcgggttgcg atggcgccgt cctcttgcaa
     1441 gtcgccatct tgaacccact tgggcaccga tgcccgtttg gcatccttgc cttccccttc
     1501 ctgccagtag gggccgaagc gaccgctcag cagttgaatc ggctcaccgg tattcggacc
     1561 gggaccgagg gtctttgctt caggttgttg tttgccgttg gcgatcaggg ctttcacgtc
     1621 ggcatcggtg atcgagccgg gggtccaccc atacggcaag ctgacaagct gtccgtcttt
     1681 aagaaccaca tacggaccgt agggaccgat cataatgggg ttcgcctcat cgatgtggca
     1741 gagattcaac gtttttgact cgcccttgtt gtccaaagtc agctgctgtc tgttctgctc
     1801 catcaatccg tgttcaccga gaaaaaactc cgccaggaat gtttctttct tcagttcgcc
     1861 ccgggctatt ttatcaaggt cctcctccat ccggactgtg aagtcgtagt cgatgaactg
     1921 tttgaaattc gtttcaagga attggcacac cccgaagccg atgaacgtcg gaacgagtgc
     1981 acttccctct ttgaccacgt acttgcgatc gagcaaggtc ttaatgatcg tcgcatacgt
     2041 tgaaggccgg ccgatccctc gcttttcaag ctcctggacg agcgatgctt ccgtataccg
     2101 ggccggcagg cgggtttggt gttccaccgc agtcaagctc ttcagcggac agacctcgcc
     2161 ttcactgagc ggagggagaa tcttgtcctt gtcgatttcg ttgtcttcca cttcttcgac
     2221 ataggcgcgg ataaagcccg gaaagacgat ctccgttccg gttgcggtaa agattcccgc
     2281 gcccgcttca atcgtcgccg tggttgtcgc cttttgagcg tccgccatct gacatgccag
     2341 cgtccgcttc cagatcaggt catagagggc cagctcccgc ccgcttaaac gagtatcctt
     2401 cggatgaaca aaggcatcac cggcgggacg gatcgcctca tgggcttcct gcgcctgggc
     2461 gctttttgcg gcaaagtatc gagccttggg gctgagatag tccgttccgt agagctcctc
     2521 cacctgagtt cgggccgcca tcgtcccctc ccggctgagc gaggggctgt cggttcgcat
     2581 ataggtgata tacccacttt catagagccg ctgggcgatt cgcattacct ccgaagcgct
     2641 gatcctgagt tttctcgtcg catcctgttg aagggtgctg gtggtgaagg gaatggaggg
     2701 acgggtgatg aacggcttgc gctcgatcgc ggtgacgaca aactgctctt ctttcaactc
     2761 tttgacgatc gcccgggctt cctcttctcc gaggtggagc gtccccttct tccctttata
     2821 ggctccggtc gttgcatcga aatctctgga ggaagcaata gcccggtcct gataggagac
     2881 gagtttcgcc tcaaagagct cgcctttggc attggtcagc tcggcgcttg catcccagta
     2941 ggtgttttgt tcgaaggcga ttctcatgcg ctcccgatcc acggtaaggc gcaaacccac
     3001 cgactgcacc cggccggcgg aaagcttctt gttggagagt ttcttccaca gcgtggggga
     3061 aagggtatac ccgtaaagcc gatcgacgag gcgccttcct tcctgggcgt gcaccagatt
     3121 ctcgtccaag tcccgccccg tttccaaggc gttggtgatc gccttcttcg tgatctcatg
     3181 gaacaccatc cgccggtagg gaatcttcgg tttcaacact tcaagaaggt gccaggagat
     3241 cgattcaccc tcgcggtctt catccgtcgc cagaagaagc cgatcgg
//
LOCUS       DAOPUZ010000098         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_29945_length_3273_cov_3.107831, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000098 DAOPUZ010000000
VERSION     DAOPUZ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29945_length_3273_cov_3.107831"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PKN79_02440"
     CDS             complement(<1..215)
                     /locus_tag="PKN79_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94651.1"
                     /translation="MKKQDAWIQSDGAVSVLVVLLGFLVGTILVALVGKNPLNMYKAI
                     LQALSGYNIDNGKMNVRYIGETLNYSVP"
     gene            complement(212..1741)
                     /locus_tag="PKN79_02445"
     CDS             complement(212..1741)
                     /locus_tag="PKN79_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ94652.1"
                     /translation="MHEYAIEMRGITKTFPGVIANDKVNLSVRNQEIHALLGENGAGK
                     STLMSILFGAYSADSGTILINGEEVEIKNPNVATALGIGMVHQHFKLVQNYTVTENIV
                     LGMEPRRGQMLDLKKARSEVAALSARYGLAVDPDAKIEDISVSQQQRVEILKTLYRSA
                     QIIIFDEPTAVLTPQEIDELIEIIVKLKDEGKTIVLITHKLREIKAVADRCTVLRRGK
                     YIGTVEVADVSEGDLAEMMVGRAVRFEIDKPEQESGDVVLKLENVSVKDHLGLEKVSN
                     LTLDVHSGEVVGIAGVDGNGQSELLGAITGLLPLSGGEIYINDRPIGGLTIRERIEAG
                     LGYTPEDRQRYGLVADFTIAENAVLKNYYHPRYTGRFGILNDQAMHDEAKTLIEQFDI
                     RAGEGSHTKAGSLSGGNQQKVIVAREISLKPKVLIVAQPTRGLDVGAIEYIRKRIIEE
                     RSRGQAILLVSFELDEIMNLCDRIATISKGTIVAVNRSDEVTERDIGMMMAGSRMKES
                     V"
     gene            complement(1832..2935)
                     /locus_tag="PKN79_02450"
     CDS             complement(1832..2935)
                     /locus_tag="PKN79_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ94653.1"
                     /translation="MKKVTILLVVLIVGAFLFAAGTKEVAKSDGRPTIRLITDATGID
                     DKSFNAAAWRGITEFYGESVGNTPSRGKLYDVVTSQTQDAYIPNLRDAAEDGSFDLIM
                     VTGFTFADALGEIAPQYPDQKFTIVDVDWVGHPNVMEFIYSEEEGSYLVGLAAALQAK
                     EDGIANPRFGFIGGVPGSTITKFEMGYVQGIRSVFPNAQIIDYYANDWGKPELAKAQA
                     KNWFDSGVYAIFSAAGGTGNGTIAQAKEYRAQGKNVWAIGVDSDQYEEGIYSGTKSAV
                     LTSMLKRVENSSLHVLNAVADGTFKGGVIQMGMVDDGVGYSTANPELSKNVIAAVEKA
                     KADVISGKVKLYKTYKDALAAGAAPAGLAALDD"
BASE COUNT          629 a          956 c          881 g          807 t
ORIGIN      
        1 ggaaccgaat agttcaacgt ctcgccgata tagcggacgt tcatttttcc attgtcgata
       61 ttgtatccgc tgagcgcctg aagaatcgct ttatacatat tcagcgggtt ctttcccact
      121 agggcgacga gaatcgtccc gaccagaaac cccaagagga cgacgagcac gctcaccgct
      181 ccgtcacttt gaatccaagc atcctgtttc ttcatacgga ctccttcatc cgggagccgg
      241 ccatcatcat gccgatatcg cgttcggtca cttcatcgct cctgttcacc gccacgatgg
      301 ttcccttgct gatcgtggcg atccgatcgc agaggttcat gatctcgtcg agctcgaagg
      361 agacgaggag aattgcctgg cctctgcttc gctcttcaat gatccgcttt cgaatgtact
      421 cgatagcccc gacatccaag cctcgggtcg gctgagcgac gatcaggact ttgggcttca
      481 gggagatctc ccgggccacg atgactttct gctggtttcc tccgctgagg ctgcccgctt
      541 tggtgtggga accctccccc gcccgaatgt caaactgctc gatcagggtc ttggcctcat
      601 cgtgcatcgc ctggtcattg aggattccga accttcccgt gtatcggggg tgatagtagt
      661 ttttcagcac cgcgttctcg gcgatcgtga agtcggcgac gaggccgtag cgctggcggt
      721 cttccggcgt atagccgagc cccgcctcga tccgctcccg gatcgtgagc cctccgatcg
      781 gccggtcgtt gatgtagatc tcgccgccgg aaagaggaag caggccggtg atcgccccga
      841 ggagttcgct ttgcccgttg ccgtcgactc cggcgattcc gacgacctcg ccgctgtgga
      901 catccaacgt aaggttggag actttctcca gtccgagatg gtctttcaca ctgacgtttt
      961 ccaacttcag gacaacgtcg ccggattcct gctcgggctt gtcgatctca aagcggaccg
     1021 cccgcccgac catcatttca gccaaatcac cttcactgac gtcggcaact tcaaccgtac
     1081 cgatatattt gccgcggcgc agcacggtgc accggtcggc gaccgccttg atctcccgca
     1141 gcttgtgggt aatcaggacg atcgtctttc cctcgtcttt gagcttgacg atgatctcga
     1201 tcagctcatc gatctcctga ggagtgagca ctgcggtcgg ctcgtcgaag atgatgatct
     1261 gtgcacttcg atagagggtt ttcaggattt cgacccgctg ctgctggctg acggagatgt
     1321 cttcgatctt cgcatcggga tcgaccgcca gaccgtaccg ggccgaaagg gcggcgacct
     1381 cgctccgcgc cttcttcaag tcgagcattt gcccccgcct cggttccatg cccaagacga
     1441 tgttctcggt gacggtgtag ttttgaacca gtttgaagtg ctggtgaacc atcccgatcc
     1501 ccaacgccgt ggcgacattg gggtttttga tctccacctc ttcaccgttg atgagaatcg
     1561 ttccgctgtc ggcgctgtag gccccgaaaa ggatgctcat cagggtcgat ttaccggcgc
     1621 cgttttcccc caaaagcgca tgaatctcct gattcctgac gctgaggttc actttgtcgt
     1681 ttgcgatgac tcccgggaag gtcttcgtta tcccccgcat ctcgatagcg tattcgtgca
     1741 cccgtctctc cttgattccg tcttcctcta cccgccatcg ccgatggcgg gcagaaggag
     1801 atgccggcag aaatccgccg gcaacagagc atcagtcgtc caatgcggcg aggccagcgg
     1861 gagcggcgcc ggcggcaaga gcgtccttgt aggtcttgta cagcttgacc ttgccgctga
     1921 tgacgtccgc cttggctttc tccacggcgg cgatgacgtt cttggagagc tcgggattcg
     1981 ccgtcgagta cccgacaccg tcgtcgacca ttcccatctg gatcactccg cccttgaagg
     2041 tgccgtcggc aacggcgttc aggacgtgga gggaggaatt ctcgacccgc ttgagcatcg
     2101 aggtcagaac ggcgctcttg gttcccgaat agattccttc ttcatattgg tcggagtcga
     2161 caccgatcgc ccagacgttt ttcccctgag cgcgatattc cttcgcttgg gcgatcgttc
     2221 cgtttccggt tccgcccgct gcgctgaaga tcgcatacac accgctgtca aaccagttct
     2281 tggcttgagc ttttgcaagc tccggcttgc cccaatcgtt ggcgtaatag tcgatgatct
     2341 gggcgttggg gaagaccgag cggattccct gaacatagcc catctcgaac ttcgtgatcg
     2401 tcgagccggg gactccgccg atgaacccga agcgcgggtt ggcgattcca tcctctttcg
     2461 cctgcagagc agcagcgaga ccgacaagat aggagccttc ttcttccgag tagatgaact
     2521 ccatgacgtt cggatggcct acccagtcga cgtcgacgat cgtgaacttc tgatcggggt
     2581 actggggagc gatttcaccg agggcatcgg caaaagtgaa gccggtaacc atgatcagat
     2641 cgaagcttcc gtcttcagcg gcgtctcgaa ggttggggat atatgcgtcc tgcgtctggc
     2701 tggtgactac atcatacagt tttccgcgcg agggagtgtt tccgacgctc tcgccgtaga
     2761 actccgtaat tccccgccag gcggcggcgt taaaggattt atcatcgatt cccgtcgcat
     2821 cggtgatcaa tcggattgta ggtcttccat cgcttttggc aacctctttg gtaccggcgg
     2881 cgaacaggaa cgctccgaca ataaggacaa cgagtaaaat cgttactttc ttcatggata
     2941 accctcctta tggtttgtcc gttatagaat gttagcattg taacacactt gggcattctt
     3001 tacataaaga aagaggcgtt tttttaaacg gtattacccc tttatctcat gaacatggat
     3061 caatccggat aagaacaccc ctcctgtttg ttttcacatg cgaagatatc gagttctttc
     3121 cgatgaatga acagcaaaag aatgcaggaa actcgctttg ttggctgagg atatccgtca
     3181 cagatcgatc gcctgatcgt ctatgtacac ggtatgttct ctggcatcat agcggatcga
     3241 gggaaccggt ttgtcggcgt cgtcgtttct cca
//
LOCUS       DAOPUZ010000099         3272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_29959_length_3272_cov_2.790799, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000099 DAOPUZ010000000
VERSION     DAOPUZ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29959_length_3272_cov_2.790799"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PKN79_02455"
     CDS             <1..472
                     /locus_tag="PKN79_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNZ94654.1"
                     /translation="GDLAMWTHSGEFAIVGRAKDTIVLAGGENLEPVPIETKLAESEY
                     IEQAVVLGQDRKYLGALIVLNKARVAEYLKEKNIPYLEETMTHLGEVKELIGRTINDV
                     ISTKSGFKPFEHIARFKLLSKSFEVGKELSAKQELKRFEIDRMYEEEINELFAG"
     gene            521..862
                     /locus_tag="PKN79_02460"
     CDS             521..862
                     /locus_tag="PKN79_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNZ94655.1"
                     /translation="MIQIIGTKQCNETKKAIRFCREARIAHHFVDLNERSLSPGEIAS
                     ILTTYRAEELIDTASKVYRKEGYAYRVYDPLEELREKPALHKTPIIRFRGKTHLGYDE
                     AILRAWGASDA"
     gene            855..1628
                     /locus_tag="PKN79_02465"
     CDS             855..1628
                     /locus_tag="PKN79_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ94656.1"
                     /translation="MLKRAVLGSGSSGNAYYMSDGETSILLDQGFSLVELNRRLERFG
                     SSASELDAVFVTHLHPDHAKGVGILARNRDIPIHLHTKAIARNTGEFAFLGIPPKQVV
                     EVEVGEMIRLGAMRIFCFTTSHDSPGSVGWYIDHPEERVMLLTDLGKTTEEQKELAQR
                     ASVLFLEANYDEKMLEEGPYPRILKHRIRSVEGHLSNDDALTFVQESGFAGNELYFVH
                     LSSTNNDHRLLEGQAKGRVSTPFVVCEKNRWYGTIGAAQ"
     gene            1625..>3272
                     /locus_tag="PKN79_02470"
     CDS             1625..>3272
                     /locus_tag="PKN79_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ94657.1"
                     /translation="MKKKKKEPVISKKKIKRMKRSDGLKMKLVDKYTMERVVAGSASR
                     YPKRIALRTYAEEGSELTYSELKRKSDAVSLYFLEQGFVKGDRIALIGESCPNWMLAY
                     FGLTGIGLTAVPVLPEFSPKEILHILQDGGVKAVVVNARHVEKILPFVRKKPEFLIRL
                     EDLFHIPMPISGELKDRNQFLNAPGRDITRRKVDAKARQLLSEHRPKEDDPASLIYTS
                     GTTGMSKAVMLTHRNLVWNADISSDVYFKVKPGYKALSILPVSHVYEFTIGQVLALLC
                     GCEIVYLGRPPAPSSLLPALKEVRPHIIMTVPLLIEKIYRSSVLPTLKANPKLQRYLR
                     FGITRRFIFRIIGRKLIVTMGGRVRFFGIGGAPLDREVEAFLAEAKFPYALGYGLTET
                     SPLISGGKPKNHTVGVIGKIVEHLEVRLEEKDPATGIGEIAVKGPSVMQGYWNNPALN
                     EEAFTPDGFFKTGDLGFIDRKGRLAIKGRTKTMILGSGGENIYPELIEAVINNQDFVT
                     ESLVIPEGGALVALIKLDIEAFAEKMALSVNEAKTEAARYI"
BASE COUNT          787 a          882 c          945 g          658 t
ORIGIN      
        1 cggagacctg gcgatgtgga cccacagcgg cgagtttgca attgtcggcc gggcgaaaga
       61 cacaatcgtt ctcgccggag gagaaaacct ggagcctgtt cccatcgaga cgaagcttgc
      121 ggaaagcgag tacatcgagc aggcggtggt gctggggcag gatcgaaagt atctcggtgc
      181 gctgatcgtg ttgaacaagg cccgggtcgc cgaatacctg aaagagaaaa acatcccgta
      241 tcttgaagag acgatgaccc acctcggcga agtaaaagag ctgatcggtc ggacgatcaa
      301 tgacgtcatc agcacaaaaa gcgggttcaa gccttttgag cacatcgccc gcttcaagct
      361 gctttccaaa agctttgaag tggggaagga gctttcagcg aagcaggagc tgaagcggtt
      421 cgagatcgat aggatgtacg aagaggagat caacgaactc ttcgccggtt gaattgcaat
      481 taaaccctgc ctccgataca ctttcagcga ggcagtgggt atgatccaaa ttatcgggac
      541 caaacagtgc aatgagacaa aaaaagcaat tcgcttctgc agggaagccc ggattgccca
      601 ccacttcgtc gatttaaatg agcgttcgct ttctcccgga gagattgcct cgattcttac
      661 cacctaccga gctgaagaac tcatcgacac cgcttcgaag gtgtacagga aagaagggta
      721 tgcgtatcgg gtctacgatc cgcttgaaga actgagggaa aagccggcgc tgcataagac
      781 cccaatcatc cgctttcgcg ggaagaccca cctcgggtac gatgaggcga tccttcgcgc
      841 atggggggct tcagatgctt aagcgggcgg ttctcggcag cggctcgagt ggtaacgcct
      901 actacatgag cgatggagaa acctcgattc ttctcgatca ggggttctcc cttgtcgagc
      961 tgaatcgacg ccttgaaaga ttcggctctt cggcaagcga gcttgatgcg gtctttgtca
     1021 cccatctcca ccccgatcat gcaaaagggg tgggaattct ggcccggaac cgggatatcc
     1081 cgatccacct ccatacgaaa gcgatcgccc gcaataccgg cgagtttgcc ttcctcggca
     1141 ttcctccaaa gcaagtggtg gaagtcgaag tcggggagat gatccggttg ggggcgatga
     1201 ggatcttttg ttttacgaca agccacgaca gccccggttc ggtcggatgg tatatcgacc
     1261 atcccgaaga gcgggtgatg ctcttgacgg accttggcaa aacgaccgaa gagcagaaag
     1321 aactcgccca aagggcaagc gtcctctttt tggaagcgaa ttacgatgaa aagatgctgg
     1381 aagagggacc gtatccccgt attctgaaac atcggatccg ttcggtggag ggacatcttt
     1441 ccaacgacga tgccctcaca tttgtccagg aaagcgggtt tgccggcaat gagctctact
     1501 ttgtccatct ctcgagtaca aacaatgatc atagactctt ggaagggcag gccaaaggtc
     1561 gtgtctcaac tccctttgtc gtgtgtgaaa agaaccgctg gtacggaacg attggagctg
     1621 cacagtgaaa aagaagaaga aagaaccggt tatcagtaag aaaaagatca aacgaatgaa
     1681 gcgatccgac gggctgaaga tgaagctcgt ggacaagtat acgatggagc gggtcgtcgc
     1741 cggcagcgcc agccgctatc ccaagcgcat cgccctccga acctacgccg aagagggaag
     1801 cgagctcacc tacagtgaat tgaagcgcaa aagcgatgcg gtcagcttgt attttcttga
     1861 gcagggattc gtcaaagggg accgaatcgc cctcatcggc gaatcatgtc ccaattggat
     1921 gcttgcatac ttcggcctga cgggcatcgg ccttaccgcc gttcctgttc ttcccgagtt
     1981 ttcacccaaa gagatccttc atatccttca ggacggcggg gtgaaggcgg tcgtggtcaa
     2041 cgcccgccat gtcgagaaga ttcttccctt tgtccggaag aaaccggagt ttctgatccg
     2101 cttggaggac ctgttccaca ttccgatgcc gatcagcggg gaactgaaag acaggaatca
     2161 atttctcaat gctcccgggc gggatatcac tcgccggaag gtggatgcca aagcccggca
     2221 actcctttcc gagcatcgcc ccaaggagga tgatccggct tcgctgatct ataccagcgg
     2281 aacgacgggg atgagcaagg cggtgatgct gacccacagg aacctggtgt ggaatgccga
     2341 catctcatct gatgtgtatt tcaaggtcaa gccggggtac aaggcgcttt ccattctgcc
     2401 ggtcagccat gtgtatgaat tcaccatcgg ccaagttctg gcgctgctct gcggctgcga
     2461 gatcgtctac ctcggccgtc ctcccgctcc ctccagcctg ctccccgcgc tcaaggaagt
     2521 ccggccccat atcatcatga ccgtcccgct gctgatcgag aagatctacc gctccagcgt
     2581 cctgccgacg ttgaaggcga acccgaagct tcaacgctac ctgcgcttcg gaatcacccg
     2641 ccggttcatc ttccggatca tcgggcggaa gctcatcgtt acaatgggcg gccgagtccg
     2701 cttcttcggg atcgggggcg ctccgcttga ccgggaggtc gaggcgtttc tggcggaagc
     2761 gaagtttccc tacgccctcg gctacggctt gacggaaacc tcccccctaa tctccggggg
     2821 aaagccgaag aaccataccg tcggtgtgat cggaaagatc gtggagcacc ttgaagtccg
     2881 gctggaggaa aaggatcccg ctaccgggat cggcgagatc gccgtcaaag gaccatcggt
     2941 gatgcaggga tattggaaca accccgcctt gaatgaagag gcgttcaccc ccgacgggtt
     3001 cttcaagacc ggagacctcg ggttcatcga caggaaaggc cggctcgcga tcaaggggcg
     3061 gaccaagacg atgattctgg gcagcggcgg cgagaacatc tatcctgagc tgattgaagc
     3121 ggtgatcaac aaccaggact tcgttaccga atcgctggtg atccccgaag gcggagcgct
     3181 tgtcgccctc atcaagctgg atatcgaggc gtttgccgag aagatggcct tgagcgtgaa
     3241 tgaagcgaag accgaagcgg cgcgctatat ca
//
LOCUS       DAOPUZ010000100         3225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_30555_length_3225_cov_3.939432, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000100 DAOPUZ010000000
VERSION     DAOPUZ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30555_length_3225_cov_3.939432"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..362
                     /gene="yajC"
                     /locus_tag="PKN79_02475"
     CDS             3..362
                     /gene="yajC"
                     /locus_tag="PKN79_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNZ94658.1"
                     /translation="MTVGLIIAIFYFLIIRPQKKREKETKEMLASIKKGDKVVSIGGI
                     HGTVMIVKETTVVIKVDDNTRIEFSRNAISSIVDRKSDAPPTARKKAKKTEEAAVVEA
                     KEVDAVEAEEVEEKSEK"
     gene            381..2108
                     /gene="secD"
                     /locus_tag="PKN79_02480"
     CDS             381..2108
                     /gene="secD"
                     /locus_tag="PKN79_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNZ94659.1"
                     /translation="MKKRNRLAIVLVVVALSAYFLFPTIKWYGFVPQEVKELATGSNV
                     QIKEYARGQATRELRALKDLVAKNADAPLPSEYSFLKSAAKENYKAMRQPVPKKWTVR
                     ALFAGFYNEQNMFDAIESYHRTRLLELKALGSKALQLGLDLRGGMSILLEADVDSYVA
                     KKGAPASASEISQAVRQDIEILEKRIDQFGVSEPMIRLQGSDQILIEIPGAADPERVN
                     SFLRGKGSLLFKMVDSELSAQLEQYYLANPGEVYTETGALKQPEFLPAGKMAAGYYVS
                     DEYGIDELARFVVIEEEIGLDGIHLESATTATDPITGRPVVNFKLDNLGGDIFYKLTS
                     TNVGKTLAVVMDGKVKAMATINEGIRSSVQISGFTAEEAADLAIVLRTAALPIELTVS
                     SQQAVGATLGEDAISVGLKAIALGFLLVVILMLAYYGLSGLVADLALLLNMFIMVSFL
                     SAFNFTVTLTSIAGLILTLGMAVDTNVIIFERIKEERRMGKSDSAAIAAGFNKAFWTV
                     MDANITTIIAALVLSQLGSSAVKGFANTLAIGIVSSVFTALFVSHLVFDAAVGDREGK
                     KLSISWRKK"
     gene            2108..>3225
                     /gene="secF"
                     /locus_tag="PKN79_02485"
     CDS             2108..>3225
                     /gene="secF"
                     /locus_tag="PKN79_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNZ94660.1"
                     /translation="MLKTDTVPVIKLRWFTLSLSIILLAAGVLSIVLFGGFNLGVDFE
                     SGLSQRIQIAPTGLEVAYDGDLDTVLSISANALTVEQRGEGGVATKLLRFSEYPTSGD
                     IATALGGIEGISVRVIDGSLKSGNLLSGYGFPATLGNEAIGLNFRTSGTTSIEEFRTA
                     LSDLGNVRVQTVGDSDSRTFQIRLGIKEGSNQASLEAEVADALGKTFGKDGFVILQSD
                     YIGPKFSATLISSSILAISVALALILVYVWIRFRFAFAVSAIVALVHDVLMMLTFISL
                     LRLEFSSTTIAALLTIIGYSLNNAIVIFDRIRENTGLNANMGLRANIDRSVRQSLSRT
                     LMSSLTTLLAILPLAIFASGDIQLFAIKMSIGLVSGVYS"
BASE COUNT          731 a          893 c          884 g          717 t
ORIGIN      
        1 tcattacggt cggactcatt attgcgattt tctacttcct gatcatccgc ccgcagaaaa
       61 agcgggagaa ggaaacgaag gagatgctcg cctcaatcaa gaaaggagac aaagtcgtct
      121 cgatcggtgg aatccacggg acggtgatga tcgtcaagga gaccacggtg gtgatcaaag
      181 tggatgacaa cacccgaatc gagttctccc gcaacgcgat cagctcaatc gtcgatcgaa
      241 agagcgatgc accgccgact gctcgaaaaa aggcgaagaa aacggaagaa gctgccgttg
      301 tcgaagcaaa agaagtcgat gcggtagaag ctgaagaagt tgaagagaaa tcggaaaagt
      361 aaccgctcct ggagataatc atgaaaaaac ggaatcgttt ggcgatcgtc ctcgtggtcg
      421 tcgccttgtc ggcatacttt ttgttcccga cgattaaatg gtacggcttt gttccgcaag
      481 aagtcaaaga acttgcgacc ggctcgaatg tgcagatcaa ggaatacgcc cgcggtcagg
      541 ccacccgaga gcttcgtgcg ctgaaggatc ttgtggcaaa gaatgcggat gcacccctgc
      601 cgagtgaata ctctttcttg aagagcgcgg caaaggaaaa ctataaagcg atgcgccaac
      661 ccgttccgaa aaaatggacg gttcgggcgc tgtttgccgg cttctacaat gaacagaaca
      721 tgttcgacgc gatcgaatcg tatcatcgca cccggcttct tgagctcaaa gccctaggaa
      781 gcaaggctct ccagctcggt ttggacctgc gcggcggaat gagcatcctc ctggaagcgg
      841 acgtggattc ctacgttgcc aagaaaggag ctcccgcctc tgcaagcgag atttcccaag
      901 cggtccgcca ggatatcgag atcctggaga aacggatcga ccagttcggc gtgagcgagc
      961 cgatgatccg gcttcaaggc agtgaccaga tcctcatcga gattcccggc gccgccgatc
     1021 ccgagcgggt gaactccttt ctccgcggaa agggatccct cctcttcaag atggtcgaca
     1081 gcgagctctc cgctcagttg gagcaatact atctggccaa ccctggtgaa gtgtatacgg
     1141 aaaccggagc gttgaagcag ccggagttcc ttcccgccgg caaaatggcc gccgggtact
     1201 acgtcagcga tgagtacggc atcgatgagc ttgcccgttt cgtcgtcatc gaagaggaga
     1261 tcgggcttga cggaatccac cttgaaagcg cgacgaccgc caccgacccg atcaccgggc
     1321 gcccggtcgt gaatttcaaa ctggacaacc tcggtggaga catcttctac aagctcacct
     1381 ccaccaacgt cggcaaaacc cttgcagtcg tcatggatgg aaaggtcaag gcgatggcga
     1441 cgatcaatga agggatccgc tccagcgtcc agatctccgg cttcaccgcc gaagaagcgg
     1501 ctgatcttgc gatcgtcctt cgaaccgccg cccttcccat cgagctgacc gtctccagcc
     1561 aacaggcggt cggagcgacg ctcggtgaag acgcgatctc cgtcggcttg aaagcgatcg
     1621 ctctcggctt ccttcttgtg gtcattctaa tgttagccta ctacggcctc agcggcttgg
     1681 tggcggatct ggcgcttctg ttgaatatgt tcatcatggt aagcttcctt tcggcattca
     1741 atttcaccgt aacgctgacc agcatcgccg gcttgatcct caccctcggc atggcggtcg
     1801 atacgaacgt catcatcttt gagcggatca aagaagagcg ccgcatgggc aagagcgaca
     1861 gcgctgcaat cgccgccggg ttcaataaag cgttctggac cgtcatggac gcgaacatca
     1921 cgacgatcat cgctgcgttg gttctctctc aactcggctc ttccgcagtc aagggatttg
     1981 ccaacacctt ggcgatcggt atcgtcagct cggtgttcac cgcgctcttc gtatctcacc
     2041 ttgtttttga tgcagcggtg ggtgatcgcg agggtaagaa actctccatc agttggagga
     2101 agaaataatg ttaaagaccg atactgttcc tgtcatcaaa cttcgctggt tcaccttgag
     2161 cctgtcgatc atcctccttg cagcgggagt tctctccatt gttcttttcg gcggcttcaa
     2221 tctgggagtc gactttgaaa gcggattgag ccaacgaatc cagattgcgc cgaccgggct
     2281 tgaggttgcc tatgacggtg atctcgatac ggtgctttca atttcggcaa atgcccttac
     2341 cgttgaacaa cggggcgagg gcggggtagc gacaaaactg cttcgcttca gcgaataccc
     2401 gaccagcggc gatatagcga ccgctttggg cggcattgaa gggatttccg tccgggtaat
     2461 cgacggatcg ttgaaatccg gcaatctgct ctccggttac ggctttcccg caaccttggg
     2521 caatgaagcg atcgggctca acttccgcac ctccggtacc acttcgattg aagaattccg
     2581 caccgccctc tccgatcttg gaaacgttcg ggtccaaacc gtcggcgaca gtgacagccg
     2641 gacattccaa atccgccttg gcatcaagga agggtcaaac caggcttcat tggaggcgga
     2701 ggttgcagat gctctcggaa aaacctttgg taaagatgga tttgtcatcc tgcaaagcga
     2761 ctacatcgga ccgaagttca gcgcgacgtt gatctcctcc tcgattttgg cgatttccgt
     2821 ggcgctggcg ctcattctgg tgtacgtgtg gattcgtttc cgcttcgcct tcgcagtctc
     2881 cgcgatcgtg gcgcttgttc acgacgtgtt gatgatgctg accttcatca gcctgctcag
     2941 actggagttc tcctccacca cgattgcggc gctgttgaca atcatcggat actccctgaa
     3001 caacgcgatc gttattttcg accgaatccg ggaaaacacg ggcttgaacg cgaatatggg
     3061 tcttcgggcc aatatcgacc gctcggtgcg gcagtcactc agccgaacgc tgatgagctc
     3121 cttgacgacg ctgctggcga ttctccctct ggcgatcttc gccagcggag atatccagct
     3181 ttttgcaatc aaaatgagca tcggtctggt ttccggggtg tactc
//
LOCUS       DAOPUZ010000101         3213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_30724_length_3213_cov_2.318239, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000101 DAOPUZ010000000
VERSION     DAOPUZ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30724_length_3213_cov_2.318239"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PKN79_02490"
     CDS             <1..809
                     /locus_tag="PKN79_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNZ94661.1"
                     /translation="ANLPSGEFVANPLMIEKEHRRFCLITGPNMAGKSTYLRQNALIV
                     LLAQIGSYVPAAKATLSLVDRLYCRVGASDNLARGESTFLVEMLEAAHILRTATSASL
                     VIIDELGRGTSTQDGMSIAYAVMRELIHIGSKTLFATHYHELARTEVQNLQLLTLEVL
                     EQGKEIRFLRRVIEGVANSSYGLNVAKMAGIHPGILKNARSFQRQHFADYDLSTLQMN
                     LFSEREDTPSSYNEVEEQVLVDLESFAIEHATPVDALLFLKELQERLKAK"
     gene            832..1419
                     /locus_tag="PKN79_02495"
     CDS             832..1419
                     /locus_tag="PKN79_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94662.1"
                     /translation="MYCIGCDRISDELLCPSCDRQVQTGSYAYLYDRRCPDCHLPIFD
                     SAYPCSWCTDGRIGWGGYEGLLARLLTLHKSRKDRRLVHYLAKLLVPMMSMIAPDLFI
                     PVPASEGGCRQRGYDQMVLFSKELSRQMGIPSRPVLIQKKGAIVLLTRNMRIENKRIV
                     LLDDIYTAGKTSSDCRKILKETWGIDPFVVTIAES"
     gene            1538..2038
                     /locus_tag="PKN79_02500"
     CDS             1538..2038
                     /locus_tag="PKN79_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HNZ94663.1"
                     /translation="MLSKGIQETPLFVTFDPLIKAVNLRLVDLSVFESRGHVGVNLVI
                     MAENRNTTVEDCATVHKIVAPRLNVLYDTREVGIEVSTPGLQRNLKDVYEFTLYGGKR
                     VRIYSTILKGWISGRIESVQENSLTLNDVFVEDTKEQLERKSVEFDEIQKAKLDYRWE
                     DRPHGN"
     gene            2028..>3213
                     /gene="nusA"
                     /locus_tag="PKN79_02505"
     CDS             2028..>3213
                     /gene="nusA"
                     /locus_tag="PKN79_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNZ94664.1"
                     /translation="MATDIGDAVRAMVAEKNISEELVISTIEDILRAAFKRKFGTDEN
                     AVIQFSDDFSTVELASRRLVVDDDNWYNEVTEIPYSEAVGLHEGATIGDELLIPVDLK
                     DFDRISVQSAKQRAHQSFRDIQKDTLYSEYKAKEGMVIIGYFRRQLPNGDIYVDIGNT
                     EGLLPKRNQSSRESYQSGDKIKCYVEKVEKAERGLRVILSRSSPELLRRLFEVEVPEI
                     AQNQVDIIKIVREAGYRSKVAVASRNDEIDPVGACVGLKGNRIQTIMSEIEGEKIDIL
                     RYDPNPVNYIRNALSPAQVRDVVIIDKNIYHAVAIVDESQLSLAIGKQGLNVRLANKL
                     VDWMIDVKTQEQFEEMDIAKEARYRAESIFIDEDGQQYYSDEQPVHFEEPMFAEEERM
                     DLG"
BASE COUNT          818 a          809 c          880 g          706 t
ORIGIN      
        1 aagctaacct gccttccggc gagttcgtcg caaatcccct gatgattgaa aaagaacacc
       61 ggaggttttg tttgatcacc ggccccaaca tggctggaaa atcgacgtat cttcggcaga
      121 acgccctcat cgttcttctc gcgcaaatcg gttcctacgt tcccgctgcc aaggcgacgc
      181 tttccctggt cgatcgcctc tattgccggg taggagcaag cgataacctt gcccgcggcg
      241 agtcaacgtt ccttgtcgag atgctggagg cggcgcacat cctgagaacc gccacatcgg
      301 catcgcttgt catcatcgac gagcttggac ggggaacatc gacgcaggac ggcatgtcga
      361 tcgcctatgc ggtgatgaga gagctcatcc acatcgggtc caaaacgctc ttcgccaccc
      421 attatcatga gcttgcccgg accgaagtgc aaaaccttca gctgctcacc ttggaggtgt
      481 tggaacaggg aaaggagatt cgcttcctca ggcgggtcat cgaaggggtt gcaaactcct
      541 cctatggctt gaacgtcgcc aaaatggcag gcattcatcc cggcatcctt aaaaacgccc
      601 ggagtttcca acgacagcac ttcgccgact acgatctttc cacccttcag atgaatctct
      661 tcagtgaacg ggaggatacg ccttcttcct acaatgaggt cgaagagcag gttctggtgg
      721 atctggaaag cttcgcgatc gaacatgcga cgccggttga cgcgctgctc ttcctgaagg
      781 aactgcagga acggttgaaa gcgaagtagg accttccgcc ccataggagg catgtactgt
      841 atcggatgtg atcggatcag cgatgaactc ctttgcccct cgtgtgacag gcaggtccaa
      901 acgggatcgt acgcatatct ctatgaccgg cgctgccctg attgccacct gccgatcttc
      961 gattccgcct atccgtgctc ttggtgcacc gacgggcgga tcggctgggg agggtatgaa
     1021 gggctgctcg cccgactgct tacccttcat aaaagtcgaa aggaccggag actggttcac
     1081 tatctcgcca agcttcttgt tccgatgatg agcatgatag cacccgatct cttcattccg
     1141 gttcccgcct cagagggagg ctgtcgtcaa cggggatatg atcagatggt gctgttctcc
     1201 aaggaattaa gccggcaaat gggaatccca agccgaccgg tcttgattca aaagaagggg
     1261 gcgatcgttc ttctcacgag gaacatgaga atcgagaaca agcgaatcgt tcttctggat
     1321 gacatctata cggcgggaaa gacgagctcc gattgcagga agatcctcaa agagacctgg
     1381 gggattgatc cattcgtggt gacgatcgcc gaatcatgaa tatacgaagg ttctgttgac
     1441 acgttttggc tatcttatta tgatacagac aggatttatt gattattggg ggatcgccga
     1501 aaggtgcatc ccctttttat ggagcaacaa aacgcgcatg ctaagcaaag ggattcagga
     1561 aacgccgctg tttgtcacct ttgatccgtt gatcaaagcg gtcaatctcc gcttggtcga
     1621 cctttcggtc tttgaaagtc gggggcacgt cggggtgaat ctggtgatta tggctgaaaa
     1681 ccgcaatacc acggtggagg actgtgcaac ggttcacaag atcgtcgcac cgagactgaa
     1741 cgttctctac gacaccagag aggtgggtat cgaggtttcc acccccggtt tgcagcgcaa
     1801 tctgaaggat gtgtacgagt ttacgctcta tggcggaaaa cgggtgcgga tctattcaac
     1861 gatcctcaag ggatggataa gcgggcggat tgagtcggtc caagagaact ccctgaccct
     1921 caacgatgta ttcgttgaag atacaaaaga acaattggaa cgcaaatcgg tcgagtttga
     1981 tgagattcaa aaagcgaaac tcgattaccg atgggaggat agaccacatg gcaactgata
     2041 ttggtgatgc agtacgagcg atggtcgcag aaaaaaacat cagtgaagag ctggtgattt
     2101 ccacgattga agatattctt cgtgctgcat tcaagcgaaa attcggtacc gatgagaatg
     2161 cggtgattca attcagcgat gatttttcca ccgttgagct ggccagccgc cgcctcgtcg
     2221 tcgatgatga caattggtat aacgaagtta cggaaatccc atattccgag gcggttggtc
     2281 tgcacgaagg agcgacaatc ggcgacgagc tcctgattcc ggtcgatttg aaagactttg
     2341 accggatcag cgtccagtcg gcgaagcagc gcgctcatca aagctttcgc gatatccaaa
     2401 aggacaccct gtacagcgaa tacaaagcga aagagggaat ggtgatcatc ggatacttcc
     2461 gccgccaact ccccaatgga gacatctacg tcgatatcgg caatacggaa ggtctccttc
     2521 ccaagcggaa ccaatcgagt cgggaaagct atcaaagcgg cgataagatc aagtgctacg
     2581 tggaaaaagt tgaaaaggcg gaacggggct tgagggtcat cctctcccga tccagtccag
     2641 agctgctgcg ccggctcttt gaagtggaag ttcccgaaat cgcccagaat caggtcgaca
     2701 tcataaaaat cgttcgggaa gccggttatc ggtcaaaagt cgcagtggcc tcccgaaacg
     2761 atgagatcga tcccgtcggt gcgtgcgtcg ggttgaaggg aaatcggatt cagactatca
     2821 tgagcgagat agaaggggaa aagatcgaca tcctgcggta tgatcccaat ccggtcaatt
     2881 acatccgcaa tgcgctctcc ccggcccaag tccgtgatgt cgtgatcatt gacaaaaaca
     2941 tctatcacgc tgtggccatc gtcgatgaga gccagctctc tctggctatc ggcaaacaag
     3001 ggttgaatgt ccgcctcgcc aacaagctgg tagactggat gatcgacgtg aagacccaag
     3061 agcagttcga agagatggat atcgccaaag aagctcgcta tcgagcggaa tcgatcttca
     3121 tcgacgaaga cggccaacag tattacagcg acgagcagcc tgttcatttc gaagaaccga
     3181 tgtttgccga agaggagagg atggatcttg gaa
//
LOCUS       DAOPUZ010000102         3200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_30916_length_3200_cov_3.589507, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000102 DAOPUZ010000000
VERSION     DAOPUZ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30916_length_3200_cov_3.589507"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKN79_02510"
     CDS             <1..653
                     /locus_tag="PKN79_02510"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HNZ94665.1"
                     /translation="RRITGEKAQFNKPFAKTKNALDYYHFLSHLFLRLGYAGWVILFD
                     ESELIGRYGKKARQKTYLAMDHFLDPSESLKGVFSLFAFSSSFTADVIEQKGEYETAT
                     ESFPDDAEVIHKTLDAIVQAKQLEPLSTKEIHRILEGIITFHAKAYDWSPMIDPKLLI
                     RKIEESGGYLLRSKIRSAIEYLDQLYLYHSEGTTTLTALQEETYEIPTYEELFEGE"
     gene            753..1274
                     /locus_tag="PKN79_02515"
     CDS             753..1274
                     /locus_tag="PKN79_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HNZ94666.1"
                     /translation="MNCYIIACRSGVEDKVKKHLDRFFADHEETDVVVTFPVRRMIDR
                     RRGKPLMNDLPLLPGYLLLSSENELDSYALDVKALYGTYGFLKNLDGSILLKGRDHAY
                     VDWVLRNQGIIKPSKVIYKQGEPIRIVQGPMKDFMGKIISVDYRRSRVKVEFEFAADI
                     RKVDLPVEFISHE"
     gene            1417..>3200
                     /locus_tag="PKN79_02520"
     CDS             1417..>3200
                     /locus_tag="PKN79_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNZ94667.1"
                     /translation="MGKILKEPFAKRLILMTVDGVLLVCANVIASWVVFRTRSALLNP
                     IVHINSVGILILLMLVQFYRIRISESSLDVIIRSLGSFIPALIIGVFTVWWKDGGHSP
                     WIPFLFVADVFGIGAILFVRVSYRIYLGYSTRHQSHQYPRTIIYGAGDLGTTLVRQSR
                     KGKLPFKIVGFVDDNPDYDRSSILGIRVYGTIKELVQILKEAQAKVLIIAITHIPQEK
                     MLEAVDAAKEADCEVKVIPSLFEMQDKPLEEVDVRSLDYADLLGRPLIKIDREPIKQM
                     VQGKTVLVTGAGGSIGQEICRQLLSYGPVKLLLFDIDETELHDLSLRLHDYQREWSDL
                     IFPIVGDIRNKQKVNTVFEIYQPQLVFHAAAYKHVPLQELYPEEAVQTNILGSYNVLK
                     AAKDHKAEKVVVISTDKAVNPTNVMGATKRVVELLARMLTSDETEMVSVRFGNVLGSR
                     GSMLPLFVEQIKAGVPVTVTHKDIIRYFMAIPEAVGLVFKAASMAKGGEVMVLDMGQP
                     VKIYDFAKKLVQYYGDENSKIIITGLRPGEKLYEELLADKDTTIPTDDPLVFKAKVNN
                     NIFSEEKFLKEHYDVFASGEKEELLRHL"
BASE COUNT          765 a          801 c          876 g          758 t
ORIGIN      
        1 accggaggat caccggtgaa aaggcgcagt tcaacaagcc gttcgccaag acgaaaaacg
       61 cgttggatta ctaccacttt ctttcccatc tctttttgag gctcgggtat gcggggtggg
      121 tgatcctctt tgacgaatcg gagctcatcg gccgctatgg caagaaggcg cggcagaaga
      181 cctatctggc gatggatcat tttcttgatc cctccgagag tctgaaaggc gtcttctccc
      241 tgtttgcgtt ctcttcttcc ttcactgcgg acgtcatcga gcagaagggc gagtatgaga
      301 ccgccactga gtccttcccc gacgatgccg aggtgatcca taaaacgctc gatgcgatcg
      361 tgcaggccaa gcagctcgag ccgctttcga cgaaggagat ccatcggatc ctggagggga
      421 tcatcacctt ccatgccaag gcgtatgact ggagcccgat gatcgatccg aagctgctca
      481 tccgaaagat cgaggagagc gggggatacc tgctgcgatc caagatccga agcgccatcg
      541 aatacctcga ccagctctac ctctaccaca gcgagggaac caccaccctc accgcccttc
      601 aggaggagac ctatgagatt ccaacctatg aggagttgtt cgagggggag taggggttgg
      661 ttcgaaacac cattgatgaa acgtgtcatg catgcccgtt catcaaggca ccccgtgaca
      721 accataccct tccggtgtaa gatgtctcca cgatgaactg ctatattatc gcctgccgca
      781 gtggcgttga agacaaggtg aagaaacacc tcgatcggtt ctttgcggat catgaagaaa
      841 cggatgtggt cgtgacgttt cccgttcgga ggatgatcga ccgcagaagg ggcaaaccgt
      901 tgatgaacga tttgccgctt cttcccggat acttgctgct ttccagtgaa aacgagcttg
      961 attcctatgc tcttgacgtc aaggcgctct atggcaccta tgggtttctg aagaatctcg
     1021 atggatccat tctgctcaag gggcgggatc atgcctatgt cgattgggtt ctgagaaatc
     1081 aaggaatcat caagccctcg aaggtcatct acaagcaggg ggagccgatc aggatcgtcc
     1141 aggggccgat gaaggatttc atgggaaaga tcatctccgt cgattaccgg cggtcccggg
     1201 tgaaggtcga gttcgagttc gccgccgaca tccggaaggt cgatctgccg gtcgagttca
     1261 tttcgcatga ataacgacac atgtattaca aggctcagtt ttgtaactat aatgctctca
     1321 agggtgatat tggtactata gagcaagatt acaggtgcaa atatttggtt tctatgatag
     1381 ggtatctaac acgaaggtat tcatctaggg ggaagggtgg gtaaaatcct caaagagcct
     1441 tttgccaaac ggctcatttt gatgacagtc gatggagtct tgcttgtctg cgccaacgta
     1501 attgcgtcgt gggtggtgtt tcgtacccgt tcggctctgc tcaatcccat tgtccatatc
     1561 aatagcgtcg gaattctcat tctcctgatg ctcgtgcagt tctatcggat tcgaatcagc
     1621 gagtcctcat tggatgtcat tattcgttct ttaggcagct tcatccccgc cctgatcatc
     1681 ggggttttca ctgtgtggtg gaaggatggt ggtcattctc cctggattcc gtttttgttc
     1741 gtggcggatg tgttcggaat cggagcgatt cttttcgtcc gggtgagcta tcgaatctac
     1801 ttgggctatt cgactcgaca ccaaagccac caatacccga gaaccatcat ctacggtgcg
     1861 ggtgaccttg gaacgacatt ggttcggcaa agcagaaaag ggaagcttcc cttcaagatc
     1921 gtcggctttg tcgatgacaa cccggattat gaccgttctt caattcttgg cattcgggtc
     1981 tatggaacga ttaaagaatt ggtgcagatc cttaaagaag cccaagcaaa agtcctgatc
     2041 attgccatca cccatatccc gcaagagaag atgcttgagg cggtcgatgc ggcgaaagag
     2101 gccgactgtg aggtgaaggt cattccttca ctcttcgaga tgcaggacaa gcccctggag
     2161 gaagtggatg tccgctccct cgattacgcc gaccttctcg gaagaccatt gatcaaaatc
     2221 gatcgggagc cgatcaagca gatggttcag ggcaagacgg ttctggtcac cggagccgga
     2281 ggctcgatcg gccaggagat ctgccggcag ctgctctcct acggtccggt caagcttctt
     2341 cttttcgata tcgatgaaac agagctacat gacttgtccc ttcgattgca cgactaccag
     2401 cgggagtggt cggatctgat cttcccgatc gtcggggaca tccgaaacaa acaaaaagtc
     2461 aatacggtct ttgaaatcta tcagccgcag cttgtgttcc atgccgcggc gtacaagcat
     2521 gtgccgcttc aggagctgta ccctgaagaa gcggttcaaa ccaatatcct cggttcctac
     2581 aatgtcctga aagccgccaa agaccataag gctgaaaaag tggtagtcat ctccaccgac
     2641 aaggcggtga atcccactaa cgtgatgggg gcgaccaagc gggtcgtcga actacttgcc
     2701 cggatgctga caagcgatga aacggagatg gtgtccgtga ggttcggaaa tgtcctgggt
     2761 tcccgcggtt cgatgctgcc gctctttgtc gagcagatca aggcgggggt tccggttacg
     2821 gttacccata aagacatcat ccggtacttc atggcgattc ccgaggcggt gggcctcgtg
     2881 ttcaaggcgg cttcgatggc aaaaggcggg gaggtgatgg tgttggatat gggccagccg
     2941 gtaaagatct atgactttgc caagaagctc gtccaatact atggagacga aaacagcaag
     3001 atcatcatca ccggacttcg tcccggtgag aagctctatg aggaactgct tgcagacaag
     3061 gacacgacca ttccaacgga tgatccgctg gtcttcaaag cgaaagtcaa caacaatatc
     3121 ttcagcgagg agaagttcct caaagagcac tatgatgtct ttgcatccgg agagaaggag
     3181 gagctgttga ggcacctcca
//
LOCUS       DAOPUZ010000103         3161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_31454_length_3161_cov_3.121378, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000103 DAOPUZ010000000
VERSION     DAOPUZ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31454_length_3161_cov_3.121378"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /gene="gpmI"
                     /locus_tag="PKN79_02525"
     CDS             complement(<1..1336)
                     /gene="gpmI"
                     /locus_tag="PKN79_02525"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNZ94668.1"
                     /translation="MVKPLQKLGKIKRKGPVVLLIMDGVGFGKYKEGDAVAAAVTQTL
                     DRLYRENPWTKLKAHGTAVGLPSDDDMGNSEVGHNAMGCGRVFSQGAKLVANSIETKA
                     MYEGDTWKKLIRNVKEHDSTLHFIGLLSDGNVHSHIDNLKAMVAQAKVEGVSRVRVHA
                     LLDGRDVGELSALEYFQPFEAFLEELNDEDFDARIASGGGRMVITMDRYNANWKMVEK
                     GWHTHVLGEGRLFASPTEAIETLRKETGAIDQDLPPFVIAEGGKPVGTIEDHDSVILF
                     NFRGDRALEITKAFEAEELVEFDRKRRPNVEYAGMMEYDGDLHVPAQYLVSPPAIDRT
                     LAEYLTKSGVRQFSISETQKFGHVTYFFNGNRSGKFSETLEEYVEIPSDTVPFEERPW
                     MKCAEIADRVIEEIESGTWDLIKLNFPNGDMVGHTGVYQAVVCSMEGMDLQIG"
     gene            complement(1338..3116)
                     /locus_tag="PKN79_02530"
     CDS             complement(1338..3116)
                     /locus_tag="PKN79_02530"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNZ94669.1"
                     /translation="MNIINARVESLRRAMADAGLDGWIINGTDPHQNEYVPERYRSRA
                     FISGFTGSAGLALITKEKALLWVDSRYFLQAADQIAGTVFEMMKIDTPSHPDPYTYLK
                     MNLPKGSVIGIDEATIPVSERRRIEGDFQNTLVLKPMKDILDAVWTDRPVLPEHEVVE
                     VPVNYAGSSMQEKLKQVRSLMIEAGATHLLLSTLDDIAWLTNLRGSDIPYNPLFMSYM
                     LISTERACLYTGEKRFTEQLRARLEEDLAIRPYTQLQDDLPNLWKHAVLYYNPQKTSV
                     MVEKSFGPYEKIEGREFTTDLKAQKNEVELEGMRRAHLLDGVALVNFLGRLDRENGSY
                     TELELAKRLLDERSRNEHFLGESFNAISGFGAHGAIVHYAADEQSNAKVEGDGLLVLD
                     TGGTYAFGTTDVTRTILFGTPTEEQIRDYTLVLKGHLALASARFPEGTNGYQLDVLAR
                     QHLWREGLSYLHGTGHGVGFRLNVHEGPQSISPKPIVAPLKEGMVVSDEPGIYRSGSH
                     GIRIENLLAVQEDEKTEFGTFYSFEVLTLCPLERDLIDPEMLTEEEIEMVDAYHAWVY
                     DELYELIDDEAIEYLEYATQSFLGEE"
BASE COUNT          584 a          952 c          857 g          768 t
ORIGIN      
        1 ttccgatctg caggtccatc ccctccatcg aacagacaac cgcctgatag actcccgtat
       61 gtccaaccat gtcgccattg ggaaagttga gcttgatcaa gtcccacgtg ccgctttcga
      121 tctcctcgat cacccggtcg gcgatctcgg cgcacttcat ccatgggcgc tcttcaaagg
      181 gaaccgtgtc ggaggggatc tccacgtact cctccagggt ttcgctgaac ttgccgctgc
      241 ggtttccatt gaagaagtag gttacgtggc cgaacttttg cgtttcggag atcgagaact
      301 gccgcactcc gctcttcgtg agatactcgg ccaacgtccg atcgatcgcc gggggagaga
      361 cgaggtattg ggccgggacg tgcaggtccc cgtcatactc catcattccc gcatactcga
      421 cgttcggcct ccgctttcgg tcaaactcaa caagctcctc cgcctcgaac gccttggtga
      481 tctcaagagc ccggtcgccg cggaagttga agaggatcac gctgtcatgg tcctcgatcg
      541 ttccgaccgg cttgccccct tcggcgatga cgaagggcgg aaggtcctga tcgatcgctc
      601 cggtctcctt cctcagcgtc tcgatcgctt ccgtcgggga ggcgaagagc cgcccctcgc
      661 cgaggacatg ggtatgccac cccttctcga ccatcttcca gttggcgttg tagcgatcca
      721 tcgtgattac cattcggccg ccgccgcttg caattcttgc atcgaagtct tcgtcgttca
      781 actcttccag aaacgcttca aagggctgga agtactccaa tgccgagagc tccccgacat
      841 cccgcccgtc cagaagggcg tggacccgaa ctctcgatac cccttcgacc ttggcttggg
      901 cgaccatcgc cttgaggttg tcgatgtggg agtggacgtt tccgtcgctt aagagcccga
      961 tgaagtggag cgtcgaatcg tgctccttga cattccggat gagcttcttc cacgtatccc
     1021 cttcatacat cgctttggtt tcgatcgagt tggcgaccag cttcgccccc tggctgaaga
     1081 ctcttccgca ccccatcgca ttgtgaccga cctcgctgtt gcccatgtca tcgtcgctgg
     1141 ggagccccac cgccgttcca tgagccttca gcttcgtcca gggattttcc cggtagaggc
     1201 ggtcgagggt ctgcgtcacg gcggcggcga ccgcatcccc ttccttgtat ttaccaaagc
     1261 ccaccccgtc catgatcaac aggacaacgg gtcctttgcg cttgattttt cccagttttt
     1321 gtaacggttt taccatctta ttcttctcct aagaagctct gtgtcgcata ttcaagatat
     1381 tcgatcgcct cgtcgtctat caattcatac agttcgtcat atacccatgc gtggtacgca
     1441 tcaaccatct cgatctcttc ttcagtaagc atctccgggt cgatcaggtc ccgctcaagg
     1501 gggcagaggg tgaggacctc gaagctgtag aaggttccaa actccgtctt ttcatcctct
     1561 tgaaccgcca gcaggttttc aatccggatt ccatgcgatc cgcttcgata gattccgggc
     1621 tcgtcgctga cgaccattcc ctccttgagc ggtgcgacga tcggtttggg gctgatcgac
     1681 tgcggtcctt catggacgtt gagcctgaag ccgacaccgt gaccggttcc gtggaggtag
     1741 gaaagccctt cccgccagag atgctgacgg gcaagcacat ccaactgata cccgttggtg
     1801 ccctcgggga agcgggcgga agcaagggcg agatgaccct tgagcacaag ggtgtaatcg
     1861 cggatctgct cctcggtcgg agtcccgaac agaatcgtcc gggtgacatc cgtcgtgccg
     1921 aacgcatacg tccctccggt gtcgaggacc agcaggccgt ctccttccac cttggcgttc
     1981 gattgttcat ccgccgcata gtgaacgatc gccccgtggg ctccgaagcc gctgatcgcg
     2041 ttgaaggatt cccccaggaa gtgctcgttt ctggatcgtt catcaagcag ccgctttgcc
     2101 agctccaact ccgtatagga gccgttttcc ctatccagcc gaccgaggaa gttgaccagt
     2161 gccacgccgt ccagaaggtg ggctcttctc attccttcca gttccacctc attcttttga
     2221 gctttcaggt cggtggtgaa ctcccgtcct tcgatcttct cgtagggacc gaagctcttt
     2281 tcgaccatca cgctggtctt ttgggggttg tagtagagca cggcgtgttt ccacaggttg
     2341 ggaagatcgt cctgaagttg ggtatacggc cgaattgcaa gatcttcttc caatcgtgcc
     2401 ctgagctgtt ccgtaaagcg tttttcgccg gtatacaggc acgcccgttc ggtgctgatc
     2461 agcatatagc tcatgaagag cggattgtag ggaatgtcgc tcccgcgaag gttcgtcagc
     2521 catgcgatgt catcaagtgt ggagagcagc aggtgggtcg ctcccgcctc gatcatcaga
     2581 gagcgaacct gcttaagctt ctcctgcatg cttgagccgg catagttgac gggaacctcg
     2641 acgacctcat gctccggcag caccgggcga tcggtccaga cggcatcaag gatatccttc
     2701 atcggtttga ggacgagggt gttctgaaaa tctccttcga tccgcctccg ttcgctgacg
     2761 gggatcgtcg cctcgtcgat cccgatgacc gatcccttgg ggaggttcat cttcagatag
     2821 gtgtacggat cgggatggga gggagtgtcg atcttcatca tctcgaagac cgtgccggcg
     2881 atctgatccg ccgcttgaag aaagtatctc gaatccaccc aaagaagcgc tttttccttg
     2941 gtgatcaggg ccaatccggc actgccggtg aacccgctga tgaacgccct gcttcgatat
     3001 cgttcgggga cgtattcgtt ttgatgggga tcggttccgt tgatgatcca gccatcaagt
     3061 ccggcatcgg ccatcgccct ccgaaggctt tcgacccgtg cattgatgat attcactgtt
     3121 tggtgctcct atagtgggtg atgaggcgga tcgccactcc g
//
LOCUS       DAOPUZ010000104         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_32157_length_3113_cov_3.490517, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000104 DAOPUZ010000000
VERSION     DAOPUZ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32157_length_3113_cov_3.490517"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /gene="queA"
                     /locus_tag="PKN79_02535"
     CDS             <1..922
                     /gene="queA"
                     /locus_tag="PKN79_02535"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNZ94670.1"
                     /translation="NDTFFDDRISSFPDLLEEGSILVLNNSKVRKARLYAESESGGIV
                     EFLFLSENPDRTWNAFVTKSKRQRVGKRYRFQEHEATIVAEQSDGSKVVAFASPIDEA
                     FFSRLGHVPLPPYIKRTDDPDDETRYQTIYAQTEGSVAAPTAGLHFTEDHLKRINERG
                     IEIASVTLHVGAGTFLPVRAERLEDHHMHTESYEINEAVACKVMRAKREGRKVVAVGT
                     TSVRTLESAYDEQSGMIRSGFGKTDLFIRPGFHFHVVDQLLTNFHTPESTLLVLVSTF
                     ASKGLMDEAYRHAVSERYRFFSYGDAMFIR"
     gene            complement(912..1523)
                     /gene="tmk"
                     /locus_tag="PKN79_02540"
     CDS             complement(912..1523)
                     /gene="tmk"
                     /locus_tag="PKN79_02540"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNZ94671.1"
                     /translation="MSTILKNFIVFEGLDGSGTTTQMQLLAETCDRNSRLCKATFEPT
                     DKPIGRLVRSILQKQVVTTPLALALLYAADREDHLFNPIYGIASEAEAGRLVISDRYL
                     YSSLAYQSVECGYETVAALNQFPHPEYLFFLDTPVDECLRRIAHRKTETELFEHQEFL
                     IQVKANYERIFEDLDPRVKLTRIDGLLSIEEISEIIKNTIFNG"
     gene            1622..>3113
                     /locus_tag="PKN79_02545"
     CDS             1622..>3113
                     /locus_tag="PKN79_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94672.1"
                     /translation="MRFHTRAKDIITATSILLLITAIVGWVLIFDEPTLSPASLLGDY
                     LLSRLSTEGASNLSFTSIERNLFANLTINNLSYRRGKDLSITIDKTNLEGGLFNLFLS
                     LFSSKPLLSLSFQEPKIYLGPNHKPGEEANAVPRLLRSWVRRLQLEALGTKGSFEAEI
                     PFGEIEVDDIDVALHWKLSDELPSFTIQARRLAAITPSFTLQSSHLLASLGQSGPFTF
                     KGLDTAMDVNDQRVTIANLSVEGEVLSLKDRSLNAQATLRQGLYQTGDVTVETPELSF
                     SGRYLDDRLPTVNLSVPSVFVNTPDLRAEGSHVDLHIYDLKTLLLRSERIDLTALETA
                     LTLLGIEVHLDAQENDLYYVTSTLNEGRITALGHTLAVHAFETLGQGMYQAGTIERVE
                     LQSSALGEVPNLFGIRFPLETSFLYDRFHEHIVGRAFSQALASDLFTEAFTFSLSYER
                     DRNDHSLDVLIEQKADLRLLAHAERMSDHLLDFSLTGRFNHFSLGVI"
BASE COUNT          725 a          871 c          779 g          738 t
ORIGIN      
        1 aaacgatacc ttttttgatg accgcatctc aagctttccc gatctcctgg aggaaggttc
       61 gatcctcgtc ctcaacaact cgaaagtccg gaaagcccgc ctctatgcgg aaagtgaaag
      121 cggcggaatc gtggaatttc tttttttatc cgaaaatccc gacaggacgt ggaatgcgtt
      181 cgtaacgaag agcaaacgtc agcgggtggg gaagcgctac cgcttccaag agcacgaggc
      241 gacgatcgtg gcggaacaaa gcgacggctc caaggtggtg gcgttcgctt ctccgatcga
      301 tgaagcgttt ttcagccgat tgggccacgt accgcttccg ccgtacatca aacgcaccga
      361 cgatcctgac gatgaaaccc ggtaccagac gatctatgcc caaactgaag gttcggtcgc
      421 ggccccgacc gccggtctgc actttaccga agaccactta aaaagaatca atgaacgagg
      481 catcgagatc gcatccgtca cgcttcatgt aggggcaggg acgttcctgc cggttcgagc
      541 cgaacgcctg gaagatcatc atatgcacac cgaatcgtat gagatcaacg aagcggtcgc
      601 ctgtaaagtg atgagggcga agcgggaggg gcgcaaagtc gtggcggtgg ggacgacgag
      661 cgtccgaact ctggagtccg catatgatga acaatccggc atgattcgaa gcgggttcgg
      721 taaaacggat ctctttatcc gccccggttt ccacttccat gtcgtcgacc agctgctcac
      781 gaacttccat acccccgaat caacattgct ggtgctggtt tccaccttcg cctcaaaggg
      841 gctgatggac gaggcatatc gccacgccgt ttccgagcgc tatcgattct tcagctacgg
      901 ggatgcgatg ttcatccgtt gaagatcgtg ttttttatga tttccgagat ctcttcgatc
      961 gagaggagcc cgtcgattcg agtcaatttc actcttggat ccaaatcttc gaagattcgt
     1021 tcatagttgg ctttgacttg aatcaggaac tcctggtgtt caaacagttc ggtctccgtt
     1081 tttcgatgag cgattcttct gagacactca tccaccgggg tatcgagaaa gaagaggtat
     1141 tccggatgag gaaattgatt cagggctgcg accgtttcat agccacactc aacgctttga
     1201 tacgccaatg aggagtagag gtaccggtca cttatcacca gccgtcccgc ctccgcttcg
     1261 gaagcgattc cgtagatcgg gttgaacaga tgatcttccc gatcggcggc atagagaagg
     1321 gcgagggcaa ggggtgtcgt aaccacctgc ttttgcagga ttgaccgcac caagcggccg
     1381 atcggcttgt cggtcggctc aaaagtcgct ttgcacaagc gggaattgcg atcgcacgtt
     1441 tccgccagca actgcatctg ggtcgtggtg ccggaaccgt caagtccttc aaatacgata
     1501 aaatttttca aaatcgtgct catacatcta tcctacgcta aaaatgcggc ggggtaaatc
     1561 cacattcaca gggtgtcaaa gacaagcaga tacgctatac tcatccttga ggtcaagact
     1621 tgtgagattt catacccggg caaaggatat cattaccgct acgagcatcc tcttactcat
     1681 cacagccatc gtcggctggg tattgatatt tgatgagccg accctttcac cggcaagtct
     1741 cctcggcgac tatctccttt cccgcctttc cactgaaggc gcatcgaatc tctccttcac
     1801 atcgatcgag cgaaaccttt ttgccaatct gacgatcaac aatctttcct accgacgtgg
     1861 aaaggaccta tcaatcacga tcgacaaaac gaatctggag ggcgggctct tcaacctgtt
     1921 tctttccctc ttctcctcga agccgctcct ctcgctttca tttcaggaac cgaagatata
     1981 cctcggtccc aatcacaagc ccggcgaaga ggcgaacgca gttccccgcc ttcttcgttc
     2041 ctgggtacgc cgccttcaac ttgaagcgct cggaaccaaa ggttcttttg aagcggagat
     2101 tccgttcgga gagattgagg tcgatgatat cgatgtagcg cttcactgga agctcagtga
     2161 tgagcttcca tccttcacga tccaagcccg gcgccttgca gcgatcaccc cttccttcac
     2221 gcttcaatcc tcgcatctgc tggcaagcct tgggcaaagc ggaccgttca ccttcaaagg
     2281 ccttgatacg gcgatggatg tgaacgatca gcgcgttacg attgcgaacc tttcggtaga
     2341 aggggaggtc ctaagcctga aagaccgatc tctgaacgcc caagcgaccc ttcgccaagg
     2401 cctgtatcaa acgggtgacg tgacggttga aacccccgag ctctctttca gcggcagata
     2461 tcttgacgat cgattgccga cggtcaacct cagcgtccct tccgtgtttg tcaacacgcc
     2521 cgatctccgg gcggaaggct cacatgtcga tcttcacatc tacgacctga aaacgcttct
     2581 actcagaagc gaacgaatcg atctcaccgc acttgaaacg gctcttacgc tcttgggcat
     2641 tgaagtacat cttgatgctc aagagaacga tctctattac gtcacctcca cgctgaacga
     2701 aggacgaatc acggcactcg ggcacacact cgccgttcac gcgtttgaaa ctctcggaca
     2761 agggatgtat caagcgggaa cgattgaacg ggtggagctt caaagctcgg cgcttggtga
     2821 agtccccaat ctgtttggaa tccgatttcc cttggagaca tccttcctct acgaccgttt
     2881 ccatgaacac attgtcggaa gagctttctc gcaagctctt gcttcggatt tgtttacgga
     2941 agcgttcacg ttctcacttt cctatgaacg cgaccgcaat gaccactcgc tggatgtcct
     3001 catcgaacag aaggcggatc ttcggcttct tgcccatgcg gaacggatga gtgatcatct
     3061 gcttgatttc agcctcaccg gccgcttcaa ccatttttcg ttgggtgtca tcg
//
LOCUS       DAOPUZ010000105         3087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_32527_length_3087_cov_2.976913, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000105 DAOPUZ010000000
VERSION     DAOPUZ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32527_length_3087_cov_2.976913"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKN79_02550"
     CDS             complement(<1..477)
                     /locus_tag="PKN79_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNZ94673.1"
                     /translation="MLILKKEATVEIEVRKSRFIAHAIPITSLDQIKSLVQKAREEHP
                     QAVHVVHAAVVGPKGDLYSFSDDKEPRNTAGRPAFEVLKGSGISNILVLIIRYFGGIL
                     LGTGDLVKAYGQSVRELLPLLETEELIEKTAFRVTVPYNLYELLKLQLVSLEAEIES"
     gene            complement(501..1400)
                     /locus_tag="PKN79_02555"
     CDS             complement(501..1400)
                     /locus_tag="PKN79_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HNZ94674.1"
                     /translation="MILETIERFYSWALIGILLLNLTQRKIPNSHKKRTATLYIASVL
                     LLFQLGIITILTKGWSHYLAIPLALLCVLIIFLLRKRAWPFKLRCVECSAKLDFNHVI
                     GGDENLCKTCWEIAHPQQAEQENPIEVTIDAQDVESVEQIDWEIWEPSEICVITYLFD
                     DDRVLLIDKKRGLGRGLVNAPGGHIEEDETAMEAAIREFREETRLDISDLEMVGVLNF
                     QFRDGLAEKAYVFFTRTYTGELAETDEARPFWVPISEIPYDRMWEDDLYWLPPALEGK
                     HFEGFFIFDGQTMVDKRITFKTP"
     gene            1506..2165
                     /gene="deoC"
                     /locus_tag="PKN79_02560"
     CDS             1506..2165
                     /gene="deoC"
                     /locus_tag="PKN79_02560"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNZ94675.1"
                     /translation="MKQEDIAPYIDHTVLAADATKQRIEQLCKEAIKYNFASVCVNGC
                     WVKTCAKLLEKSSVKVCTVVGFPLGAMSSEAKAYEAKKAISAGASEIDMVINIGFLKD
                     RQDDKVQEDISAVKAVCGRAAILKVIIETSLLNDEEKARACRLAKASGADFVKTSTGF
                     STGGATVKDVALMRKTVGPDTGVKASGGVRTYEDFLAMVGAGANRIGTSNGVAIINGA
                     H"
     gene            2152..>3087
                     /locus_tag="PKN79_02565"
     CDS             2152..>3087
                     /locus_tag="PKN79_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNZ94676.1"
                     /translation="MAHIEQSPQARDFLKSLGFPTLAFHETFSHFDFTVPGRRVLLIG
                     PMGSGKTEFAARVWRDAAIAQKKGKVVQKQTSTGEVDRRNVFFVRAQIDASRFPDAPP
                     DAMVYRNGYIRCGENIALIRDSFDLERVLADNPKVGTFIIDEASFFDERLAYVVRNHS
                     YERGVMFIFPSLILNFRRDLFNMTARLMLDIATDVIPLTAYCEHPDCLRDAFYTYRYY
                     QVGEKECPALYFDPLIIVGGDEHKDSSVEPNYAARCDEHHYLPGKEYTYFILKPLGQK
                     AAAGDEKPLINELTTLKEEIKASQLWKNFTERYEKL"
BASE COUNT          697 a          841 c          876 g          673 t
ORIGIN      
        1 gctttcgatc tccgcttcca aagaaacaag ctgcaacttt aggagttcat agaggttgta
       61 gggcaccgtc acccggaatg cggtcttctc gatgagctct tcggtctcca acaggggaag
      121 cagttcgcgg acgctctgac catacgcctt aaccagatct ccggttccca gcaggattcc
      181 cccgaagtaa cggatgatga gaaccaggat gtttgagatt ccgctgccct tgagaacctc
      241 gaaggcgggt cggccggcgg tgttcctcgg ttccttgtca tcgctaaagc tgtacagatc
      301 gcccttcggc ccaaccaccg cggcgtgaac cacgtgcacc gcctggggat gctcctcccg
      361 ggctttctga acgagacttt taatctgatc caacgacgtg atcggaatcg catgggcgat
      421 gaagcgggac ttccgcacct cgatctcaac ggtggcttct tttttaagga tgagcatatc
      481 ttctgtagga tactccatcc tcacggagtt ttgaaggtta tccgcttgtc gaccatcgtc
      541 tgtccgtcaa agatgaagaa cccttcgaag tgcttcccct ccagagcggg cggcagccag
      601 tacaggtcat cctcccacat ccggtcatag gggatctcgg agatcgggac ccagaacggc
      661 cgggcttcat cggtttcggc gagttcgccc gtataggtcc gagtgaagaa cacgtacgct
      721 ttttccgcaa ggccatcgcg gaactggaag ttcaggactc cgaccatctc cagatcgctg
      781 atgtcgaggc gggtctcttc cctgaactcc cggattgccg cctccatcgc cgtctcatcc
      841 tcttcgatgt gtccgcccgg agcgttgacc agtccccggc cgaggccgcg cttcttgtcg
      901 atgaggagaa cccgatcgtc atcgaagaga taggtgatca cacagatttc actcggttcc
      961 cagatctccc agtcgatctg ttcgacggat tcgacatcct gggcgtcgat cgtcacctcg
     1021 atcggattct cttgttcggc ctgctgcgga tgggcgattt cccagcatgt cttacagagg
     1081 ttctcatccc cgccgatgac gtggttgaaa tcaagcttcg cggagcattc gacgcagcgc
     1141 agcttgaacg gccaagcgcg cttgcgcagc agaaagatga taaggacgca caatagcgca
     1201 agcggaatgg cgaggtaatg gctccatccc tttgtcagga tcgtgatgat cccgagctga
     1261 aagaggagaa gaaccgaggc gatataaagc gttgcggttc gtttcttatg gctgttggga
     1321 atttttcgct gagtcaggtt cagcaaaaga attccgatga gagcccatga gtaaaagcgt
     1381 tcaatcgttt caagaatcat agaggtaggg tattggcagc ggggaccctc gtcaagatat
     1441 tctccaattg cccgctcatc ggatttggag taagctgtca ctacgttact tgatggagga
     1501 tctggatgaa acaagaggat atcgccccat atatcgatca cacggttctc gccgcggatg
     1561 cgacgaagca gcgtatcgaa cagctgtgca aagaggccat caaatataac ttcgcctcgg
     1621 tgtgtgtcaa tgggtgctgg gtcaaaacgt gcgccaagct gttggaaaag agctcggtga
     1681 aggtttgtac cgtggtcgga ttccccttgg gggcaatgag cagtgaagcg aaggcgtatg
     1741 aggcgaaaaa agcgatcagc gccggggcga gtgaaatcga catggtcatc aacatcggat
     1801 ttctgaaaga ccgccaggat gacaaggtgc aggaggacat cagcgcggtg aaagccgtct
     1861 gcggccgggc ggcgatcctg aaagtaatca ttgaaaccag tctgttgaac gatgaagaaa
     1921 aggctcgcgc ctgtcgcctt gccaaggcaa gcggagcgga cttcgtaaag acctcgaccg
     1981 gattttccac cggtggagcc accgtgaaag acgttgcgct gatgcgcaag accgtcggtc
     2041 ccgatacggg agtgaaagct tccggcgggg ttcgaaccta cgaagatttc cttgcgatgg
     2101 tcggagccgg agcgaatcga atcgggactt ccaacggagt ggcgatcatc aatggcgcac
     2161 attgaacaat cgcctcaagc ccgggatttt ctcaagagcc tgggctttcc gacccttgcg
     2221 ttccatgaaa cgttctccca cttcgatttc accgtgcccg gacggcgtgt tctcctgatc
     2281 gggccgatgg gctcggggaa aaccgagttc gccgcccggg tctggcggga tgcggcgatc
     2341 gctcaaaaga aggggaaggt ggttcagaaa cagacatcga ccggcgaggt cgatcgacga
     2401 aacgtctttt ttgtccgcgc ccagattgat gcttcccgct ttccggatgc accgccggac
     2461 gcgatggtct atcgaaacgg ctatatccgc tgcggcgaga atatcgccct gattcgagac
     2521 tccttcgatc ttgaacgggt attggcggac aatccgaagg tcggaacatt catcatcgac
     2581 gaagcgtctt tcttcgatga acggttggcg tacgtggtgc ggaaccacag ctatgaacgg
     2641 ggagtgatgt tcatcttccc ttccctgatt ctgaactttc gccgcgacct cttcaacatg
     2701 acggcgcgcc tgatgctgga tatcgcaacc gacgtgattc cccttaccgc ctactgcgaa
     2761 caccccgatt gtctccgcga tgcgttttac acctaccgtt actatcaggt gggtgaaaag
     2821 gagtgcccgg ccctgtactt cgatcccttg atcatcgtcg gaggggacga gcacaaggac
     2881 agctcggtgg aacccaacta tgccgcccgc tgtgacgagc atcactatct gcccgggaag
     2941 gagtacacgt actttatcct caagccgctg ggccaaaaag cggccgccgg ggacgagaaa
     3001 ccgctcatta atgaactcac cacgctgaaa gaggagatca aagcttccca attgtggaag
     3061 aacttcaccg aacggtatga gaagctg
//
LOCUS       DAOPUZ010000106         3084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_32570_length_3084_cov_3.182569, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000106 DAOPUZ010000000
VERSION     DAOPUZ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32570_length_3084_cov_3.182569"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKN79_02570"
     CDS             <1..208
                     /locus_tag="PKN79_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HNZ94677.1"
                     /translation="VHQLLIAATLEIRDNKGNTVLLEGQDALGSLDGMNLLARKTVTK
                     TIPWPNGLSSGSGVTYQATIKYTK"
     gene            376..>3084
                     /locus_tag="PKN79_02575"
     CDS             376..>3084
                     /locus_tag="PKN79_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94678.1"
                     /translation="MIVLPVEEVRVEPSPIAAEEPAVIEPETAAAPPVVFTEELPSEP
                     ISVAIGEDVGAEPPAVIEKEPASVLIVEETVPEPLSEEPMIPEEEPSGYPFGIVPIIK
                     SDDGSTSFDLVIVHTNDVKGNIYTENGGLGLARLSTLMKMGRGYSDNLLFLNTGDVGS
                     IAPEHEEIAALVVDEMGYDAYLPFEIQGTKNARSLAINALDEEGYFVVQPYQLYIYNG
                     FVVGVVGVVGPKDVPNVTFDSDLVLENARHAVDLAHTFVDYLIILTDLGDGPFSSVDL
                     AESIDGIDLIIDGNGGETVLDVNNTLIVRGKEQLRSIGGLVLTIVDREVMLTQLLTFG
                     AEDLLNPSASEILQAYGEMAEHYGTTLPEEIDEDPEIVALIGPSPEEPMIPEEEPSGY
                     PFGIVPIIKSDDGSTSFDLVIVHTNDVKGNIYTENGGLGLARLSTLMKMGRGYSDNLL
                     FLNTGDVGSIAPEHEEIAALVVDEMGYDAYLPFEIQGTKNARSLAINALDEEGYFVVQ
                     PYQLYIYNGFVVGVVGVVGPKDVPNVTFDSDLVLENARHAVDLAHTFVDYLIILTDLG
                     DGPFSSVDLAESIDGIDLIIDGNGGETVLDVNNTLIVRGKEQLRSIGGLVLTIVDREV
                     MLTQLLTFGAEDLLNPSASEILQAYGEMAEHYGTTLPEEIDEDPEIVALIGTLVEEPP
                     VEEFVGPPPPPYVFDPLVFTEEEYDFFYPTAPEPLPADDDFWADFFVVGEDSAVAYDD
                     GTYYFRLFVNEDLVGTIEIVFEGEERLVNVEDLQFYVAEHLTMAARIRIFSDLGSHVT
                     PEELERRGVETVYDEQAFVLYLTFSIQDMPERILSLSSATVNRREQYAISGAIPLKPR
                     WFSLAANLNLFGQLEYPDDFSKFNRTLLNLSVSNRASLFGVALNYNF"
BASE COUNT          694 a          784 c          862 g          744 t
ORIGIN      
        1 ggtccaccag ctcttgatag ccgcaacatt ggagattcgc gacaataagg gaaacacggt
       61 tcttcttgaa ggccaagatg ctcttggaag cttggatgga atgaacctgc tggctcgcaa
      121 aactgtaaca aagacaatcc cgtggccgaa cggattatcc agcggatcgg gtgtaacgta
      181 tcaagcaacc attaagtata cgaaatagcg caccaccttc cgaagcagaa gggactcgcg
      241 atgggcagaa aaactagtaa aagcgcagtt ttcctcggat ttcttctgag tatcggggta
      301 ttgattattg cgtttttcct ctatcacatg gtaacgagac ctcctgaaag acctgttcct
      361 cacattgagg aacaggtgat cgttttacct gtggaagagg tcagggtcga accaagcccc
      421 atcgcagccg aagagccggc ggtaattgag ccggaaacgg cagcagcgcc tcccgttgtt
      481 tttacggaag aacttccctc tgaaccaatc agcgttgcca ttggagagga tgttggcgca
      541 gagcctcctg ccgtcattga aaaagaacct gcttccgttc tcatcgtcga agagacggtg
      601 cctgagcctc tttccgaaga gccgatgatt cctgaagaag aaccttccgg gtatccgttt
      661 ggaatcgttc cgattatcaa gagtgatgat ggcagcacct cgtttgatct ggtgattgtg
      721 cacaccaacg atgtgaaggg caacatctat acggagaacg gcggtctcgg tcttgcccgg
      781 ctatcgacgc tgatgaagat gggaaggggt tactccgaca acctgttgtt cctcaacacc
      841 ggtgatgtcg gatcgatcgc ccccgagcat gaggagattg cggcgttggt agtcgatgag
      901 atgggctacg acgcatactt gccgtttgag atccaaggaa cgaagaacgc ccggtcgctt
      961 gcgatcaacg ccctcgatga agagggatac ttcgtcgttc agccgtatca gctctatatc
     1021 tacaacggct tcgtcgtcgg cgttgtcgga gttgtggggc cgaaagatgt cccgaacgtc
     1081 acctttgatt cggatctcgt tcttgaaaac gcccggcatg ccgtggatct tgctcatacg
     1141 ttcgtcgatt acctgatcat cctgaccgac ttgggcgacg gaccgttttc ttcggtggat
     1201 cttgcggagt cgatcgatgg aatcgacttg atcatcgacg gcaacggagg ggagacggtt
     1261 ctggatgtca acaataccct catcgtccgc ggtaaagagc agctgcgctc cataggggga
     1321 ttggtcttaa cgatcgtcga tcgtgaggtg atgttgactc agctgctgac cttcggagcc
     1381 gaagacctcc tcaatccgtc ggcgagcgag attcttcagg cgtacggaga gatggccgaa
     1441 cactatggaa caacgcttcc cgaagagatc gatgaggatc ccgagatcgt cgccttgatc
     1501 ggcccctcac ccgaagagcc gatgattcct gaagaagaac cttccgggta tccgtttgga
     1561 atcgttccga ttatcaagag tgatgatggc agcacctcgt ttgatctggt gattgtgcac
     1621 accaacgatg tgaagggcaa catctatacg gagaacggcg gtctcggtct tgcccggcta
     1681 tcgacgctga tgaagatggg aaggggttac tccgacaacc tgttgttcct caacaccggt
     1741 gatgtcggat cgatcgcccc cgagcatgag gagattgcgg cgttggtagt cgatgagatg
     1801 ggctacgacg catacttgcc gtttgagatc caaggaacga agaacgcccg gtcgcttgcg
     1861 atcaacgccc tcgatgaaga gggatacttc gtcgttcagc cgtatcagct ctatatctac
     1921 aacggcttcg tcgtcggcgt tgtcggagtt gtggggccga aagatgtccc gaacgtcacc
     1981 tttgattcgg atctcgttct tgaaaacgcc cggcatgccg tggatcttgc tcatacgttc
     2041 gtcgattacc tgatcatcct gaccgacttg ggcgacggac cgttttcttc ggtggatctt
     2101 gcggagtcga tcgatggaat cgacttgatc atcgacggca acggagggga gacggttctg
     2161 gatgtcaaca ataccctcat cgtccgcggt aaagagcagc tgcgctccat agggggattg
     2221 gtcttaacga tcgtcgatcg tgaggtgatg ttgactcagc tgctgacctt cggagccgaa
     2281 gacctcctca atccgtcggc gagcgagatt cttcaggcgt acggagagat ggccgaacac
     2341 tatggaacaa cacttcccga agagatcgat gaagatcccg agatcgtcgc cttgatcgga
     2401 accctggtcg aagaacctcc tgttgaggag ttcgtcggtc cgccacctcc tccatatgtg
     2461 ttcgatccgt tggtattcac cgaagaagag tatgacttct tctatccgac ggcgcctgaa
     2521 ccgctgcccg ccgatgatga tttttgggcg gacttctttg tcgtcggcga ggatagtgcc
     2581 gttgcgtatg atgatggcac gtattacttc cgcctctttg tcaacgagga tcttgtagga
     2641 acaatcgaaa ttgttttcga aggggaggaa cgcctggtca acgtggagga tctgcagttc
     2701 tatgtcgccg aacatcttac aatggcggcc cgtattcgaa ttttctccga tcttggctcc
     2761 catgtgactc ctgaggagtt ggaaagacgt ggggttgaga cggtgtatga tgaacaggct
     2821 ttcgttctgt atctgacgtt ctctatccaa gacatgcccg aacgaattct cagcctctca
     2881 tccgccacgg tcaaccgccg cgaacagtac gcgatcagtg gagcaattcc cttgaagccg
     2941 cggtggttca gcttggcggc gaatttgaac ctgttcggtc agttggaata tccggatgat
     3001 ttcagtaaat tcaaccggac gctgctgaac ctttccgttt ccaatcgcgc ttccctcttc
     3061 ggggtggcgc tgaactacaa cttc
//
LOCUS       DAOPUZ010000107         3074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_32719_length_3074_cov_4.616098, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000107 DAOPUZ010000000
VERSION     DAOPUZ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32719_length_3074_cov_4.616098"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="PKN79_02580"
     CDS             complement(<1..529)
                     /locus_tag="PKN79_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNZ94679.1"
                     /translation="MKTLLAKDKAARDLLSLGALVIRLDPGTTPFEYASSFDLHVSGG
                     EFNVAANLSRAFGQRTAIATAMVDYPLGLKIESEVRRMGVHPFYKRFEHDGVRGPNMA
                     HVYSDRGQGMRAPVVFYNRGDEAAARLDADSFDYDKLFEGGVRWVHSGGIFAALSKTT
                     PELIKEFFRRAKAQGA"
     gene            complement(540..1988)
                     /gene="gnd"
                     /locus_tag="PKN79_02585"
     CDS             complement(540..1988)
                     /gene="gnd"
                     /locus_tag="PKN79_02585"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNZ94680.1"
                     /translation="MKQAQIGLIGLAVMGENLVLNLESKGFSVAVYNRNTERVDNFLA
                     SRAKGKAIIGAHSVEELVSKVQSPRKIMLMIKAGDPVDQMIESLIPLLEAGDTIIDGG
                     NSNWEDTERRVAYVESKGLLYIGTGVSGGEEGALLGPSIMPGGSKKAWPALKPMLQGI
                     SAHVDGIPCCDWVGSGGAGHFVKMVHNGIEYGDMQLICEVYDIQRRLLGLSNKEMHKI
                     FAEWNEGELDSYLIEITKDILAYQEDDGTFLVDKILDAAGQKGTGKWTGISALHEGVP
                     LTLIVESVFARSVSALKEQRVAASKVFDLEIKAPAFDKKQVITQLQEALFAAKIISYA
                     QGFDLIKTAGITNKWDLDLGGIAQLWRGGCIIRSRFLGKITEAYRKNPDLPNLILDAH
                     FAEILKKNHQSLRAIVALAAQSGIPTPSLSAALSWFDSYRTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGRGGSTASTIYSI"
     gene            complement(2007..2861)
                     /locus_tag="PKN79_02590"
     CDS             complement(2007..2861)
                     /locus_tag="PKN79_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNZ94681.1"
                     /translation="MANGIHDISALFTIKERYDDLSAKEKQIADFILEHPRESVNPSI
                     EELAERIGISESTMVRFARKLGYSGYQRFRIALARETVPVTSQLFEREVIDASDGFDA
                     VFNSAIASLLETQKSLDRGLVLGVATLMANARNVYLMGLGGSNIVAQDALHKLVRTGI
                     NCQWAADFHLQLMMASQGTKEDVALLISHTGSGYDSIALAEELKANQVKLVVLTSNPR
                     SPLARLADYILTVKTGASGAVSESFSARIVALAIIDLLYIHVMELLDERGIEQLNRMR
                     ASIAKRRV"
BASE COUNT          631 a          849 c          893 g          701 t
ORIGIN      
        1 atgctccctg cgctttcgcc cggcggaaga actccttgat cagctcgggg gtcgtcttgg
       61 agagagcggc aaagattcca ccgctgtgaa cccagcggac tccgccttca aacagcttgt
      121 catagtcgaa gctgtcggca tccagacggg cggccgcttc gtcgccccgg ttgtagaaga
      181 ccaccggtgc gcgcattccc tggccccggt cgctgtagac gtgggccatg ttgggtcccc
      241 ggacgccgtc gtgctcgaag cgcttgtaga acgggtggac gcccatcctg cggacttccg
      301 attcgatctt cagaccgagg gggtagtcga ccatcgcggt tgcgatcgcc gtccgctggc
      361 cgaacgcccg gctgaggttc gccgcgacgt tgaactctcc tccggagacg tgcaggtcga
      421 aggacgaggc gtattcaaag ggagtcgttc ccgggtcgag tcgaataacc agtgcaccga
      481 gggaaaggag atcacgggct gctttgtctt ttgcgagtaa tgttttcata agtatcctcc
      541 tagattgagt aaatggttga agcggtcgaa ccaccgcggc cggtccagtt ggtgtggaag
      601 aactcacctc gcggcttgtc ggtccgctcg taggtgtggg ctccgaagta gtcgcgctgc
      661 gcctgcaaga ggtttgccgg gagccgctcg gtgcggtagc tgtcaaacca ggagagtgca
      721 gcactcagcg acggggtcgg aattccactt tgggcggcca gggcgacgat tgcccgcagg
      781 ctttggtggt tcttcttgag aatctcggcg aagtgggcgt cgagaatcag gttgggaaga
      841 tcggggttct ttcgatacgc ctcggtgatc ttgccgagga agcgggatcg gatgatgcac
      901 ccgccgcgcc acagctgggc gattccgcca aggtcgaggt cccacttgtt ggtgatgccg
      961 gcggtcttga tcaggtcgaa gccctgggcg taggagatga tcttcgcggc gaagagcgcc
     1021 tcctgaagct gggtgatgac ctgctttttg tcaaaggcgg gagccttgat ctcaagatcg
     1081 aacaccttgc tcgccgccac tcgctgctcc ttcaacgcgg agacgctgcg ggcgaacacc
     1141 gattcgacga tcagggtcag gggaacgcct tcatggaggg cgctgatccc cgtccacttt
     1201 ccggttccct tctggccggc ggcatcgagg atcttgtcga cgaggaaggt accgtcatct
     1261 tcctgatagg caaggatatc cttggtgatc tcgatcagat aggaatcgag ctcgccttcg
     1321 ttccactcgg cgaagatctt gtgcatctct ttgttggaaa ggcccaagag ccggcgctgg
     1381 atgtcataca cctcgcagat cagctgcatg tcaccatatt cgatgccgtt gtgaaccatc
     1441 ttcacgaagt gaccggctcc gccgctgccg acccagtcgc agcagggaat cccgtcgaca
     1501 tgggcgctga tcccttgaag catcggcttg agggcgggcc aggctttctt cgaaccgccg
     1561 ggcatgatcg aggggccgag cagggcgccc tcctcaccgc cggaaacgcc cgttccgatg
     1621 tagagcaggc ctttggactc cacataggcg actcgtctct cggtgtcctc ccagttggag
     1681 ttccctccgt cgatgatcgt atcgcccgcc tcgagcaggg gaatcaacga ttcgatcatc
     1741 tgatcgaccg gatcgcccgc cttgatcatc agcatgattt ttcggggcga ttgtactttg
     1801 ctcacaagct cttcaacgct gtgagcaccg atgattgcct ttcctttggc tcgggaagcc
     1861 aaaaaattgt caacccgttc ggtgtttcgg ttgtagaccg cgacactgaa gcctttggat
     1921 tccaagttca ggacgaggtt ctcgcccatg accgccaatc caatcaagcc aatctgtgct
     1981 tgtttcattg tacatttctc cttatgttat acgcggcgct tcgctatgga ggcgcgcatt
     2041 ctgttcaatt gttcgatccc ccgttcatcc aggagctcca tgacgtggat gtacaagagg
     2101 tcgatgatcg ccaaagcaac gatccgggcg gaaaaggact ccgagaccgc cccggaagca
     2161 cctgttttga ccgtcaggat gtaatcggcg agccgggcga gcggggagcg ggggttgctt
     2221 gtcagcacca cgagcttcac ctgattcgct ttgagctctt cagcgagggc gatcgagtca
     2281 tagccgcttc cggtatgact gatcaacagg gcgacatctt ccttcgttcc ttggctggcc
     2341 atcatcagct gaaggtggaa atccgccgcc cactggcagt tgattccggt ccgaaccagt
     2401 ttgtgcaggg cgtcttgggc gacgatgttc gatccgccga gccccatcag atagacgttt
     2461 cgggcgttcg ccatcaacgt ggcgactcca aggaccaaac cccgatcaag ggatttctga
     2521 gtttcaagca ggcttgcaat cgcgctgttg aagacggcgt caaacccgtc cgaggcatcg
     2581 atcacctccc gctcaaagag ctgactcgtc acgggaaccg tttcccgggc gagggcgatg
     2641 cggaagcgtt gatagccgct gtacccgagc ttccgggcga agcggaccat cgtcgattcg
     2701 ctgattccga tccgttcggc aagttcttca atggaaggat tcacggactc acggggatgc
     2761 tcgaggatga aatcagcgat ttgcttctct ttcgcgctca aatcatcgta tcgctccttg
     2821 atcgtgaaga gggcggagat gtcgtgaatg ccatttgcca taggtttagt atagtatgaa
     2881 ataaaaattc atacaatggc aaaaatattc atatttaatg attccttcct gaaaaaccgg
     2941 gaaaactcgg tctaattcag aaaaataaat tcaaaaatgg aaaaataaac acagatatga
     3001 aaaatacttt cagtttttcg ctccatgctt ccgcagtaac taaccaaaac taggtgagcg
     3061 tagttttttg ttta
//
LOCUS       DAOPUZ010000108         3072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_32751_length_3072_cov_3.389791, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000108 DAOPUZ010000000
VERSION     DAOPUZ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32751_length_3072_cov_3.389791"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1998)
                     /gene="rpoD"
                     /locus_tag="PKN79_02595"
     CDS             complement(103..1998)
                     /gene="rpoD"
                     /locus_tag="PKN79_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNZ94682.1"
                     /translation="MTDNDIELVEPLETNEINGFDGEPDDDEIDEVGDVFSLDLEDDD
                     IDDEIDVDVEPISVEDFHVPEEEDDEPPKKTKDDDDDDDLDEEDEDDEVDGLKSWSGI
                     STDDDSAGGAHYVDISKLDDQDGESDSSRHPTILGTDKGEGGGDDPIRLYLREIGREN
                     LLTAEEEVELSMEMEKGAAIIIEVIQESGILITRFKDILTVLSTKRDENDDEYSQKDS
                     KEFEISLKRYNNCYRESLKDIQAVLKTYIAKKDQMILSGEDILGDAALDKSRQALLKK
                     LSTIELQPEEILAFTNDFIEAEYKIRSFNEKKQQLENKLNIRTARDLRQLGKDLATQS
                     KAVLIEEELHLSSDTIKDLIRELQITHKVLKQIEFEFEEKSEVILELADRIKFGQKKM
                     KDAKDRLIRANLRLVVSIAKKYTNRGLHFFDLVQEGNIGLIKAVEKFEYRKGFKFSTY
                     ATWWIRQAITRSISDQARTIRVPVHMIEQINKVVRESRMLMQSLGREPTDEEVAEKLG
                     WTEQRVKAVKNVAREPISLETPVGEEEDSLLSDFIEDKDVENPAQQTAYSLLQEQLKE
                     VLSTLPAREQEVLKMRFGLEDSYSLTLEEVGLYFEVTRERIRQIEAKALRRLRHPQRS
                     RKLKDFI"
     gene            complement(2375..>3072)
                     /locus_tag="PKN79_02600"
     CDS             complement(2375..>3072)
                     /locus_tag="PKN79_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNZ94683.1"
                     /translation="STAKGKARAFHQLKPYLRATHSEIERQELIKTLSDLLDIDEASM
                     HEEYAKEQTPKNVVPKREAQPVVPLNPAAISLDYYLMLTVANNPELYLKTRASIGFES
                     LNDPKAHELYEALEQAAREDALAGSEYLLQLLESDQVKSDLSSSFGLAEFRNEPQKIL
                     KESLLRIRLRSWEKKRQSNKRLLDITQLEGNDSRTIEELLEERVEIEEEIARIKQELK
                     EETGRMASALLLL"
BASE COUNT          643 a          853 c          671 g          905 t
ORIGIN      
        1 ctggcgatct cctcttccag agcaaatcga atgctcaaat cattctgtaa ttgattaagc
       61 cgtgaaaata ccgctgcttc attcatcatc attcttcctc tattaaatga aatccttcaa
      121 ttttctgctt cgttgcgggt ggcggagcct ccgcaaagct ttcgcttcaa tctgtcgaat
      181 ccgctcgcgt gtaacctcaa aatacaatcc tacttcctcg agtgtcaacg agtagctgtc
      241 ctctaagccg aaccgcatct tcaacacttc ctgctcgcgg gccggaagcg tgctgagcac
      301 ctctttcagt tgctcctgaa gtaatgaata ggcggtttgc tgagcgggat tctcaacatc
      361 cttgtcctca atgaaatcac tgaggaggga atcctcttcc tcgccgaccg gtgtttcaag
      421 actgatcggt tcgcgggcga cgtttttgac ggcctttacc cgctgctcgg tccaaccaag
      481 cttttcagcg acttcctcat cggtcggctc ccgcccgagg ctctgcatca gcatcctcga
      541 ctcgcgaacg actttattga tctgttcgat catgtggacc gggacgcgga tcgttcgggc
      601 ctggtcactg atcgaacggg tgatcgcctg acgaatccac cacgtggcgt aggtggaaaa
      661 tttgaagccc ttgcggtact cgaacttctc aaccgctttg atcaagccga tattcccttc
      721 ctgaacaagg tcaaagaaat gaaggccgcg attggtatac ttcttggcga tcgagacgac
      781 cagccgcagg ttggcccgaa tcaggcgatc ctttgcatct ttcatcttct tttggccgaa
      841 tttgattcga tcggccaact cgagaatgac ttcacttttt tcctcaaatt caaactcgat
      901 ttgcttgagc actttgtgag tgatctgaag ctcccgaatc agatccttga tcgtatcgct
      961 ggaaaggtgc agctcttctt cgatcaggac ggcttttgac tgggtcgcca aatccttgcc
     1021 gagttgacgg agatcccgtg ctgttcgaat attcagcttg ttttcaagct gttgcttctt
     1081 ttcattgaag gaacgaatct tatactccgc ctcgatgaag tcgttagtga acgcaagaat
     1141 ctcttccggc tgcaattcga tcgtcgagag tttcttgagc aatgcctgtc gactcttatc
     1201 caaagcggca tcgccgagaa tatcctctcc cgagagaatc atctggtctt tctttgcgat
     1261 ataggtcttt aaaacagctt gaatgtcctt caacgattcc cggtagcaat tgttatatcg
     1321 cttcaggctt atttcaaatt ctttggaatc cttttgcgaa tattcatcgt cattttcatc
     1381 ccgcttcgta gaaagcaccg tcaggatgtc cttgaatcgg gtaataagaa ttccgctttc
     1441 ctggattacc tcgatgatga tcgccgcccc cttttccatc tccatggaaa gttcgacctc
     1501 ttcttcggcg gtaagaagat tctccctccc gatctcgcgc aaataaagtc gaatcggatc
     1561 gtccccgcca ccctcgcctt tatcggttcc aaggattgtc ggatgacggg aagagtcgct
     1621 ttccccatcc tgatcgtcga gtttggaaat atccacgtaa tgggctccac cggccgagtc
     1681 atcgtcggtg ctgatgccgc tccatgattt caacccatcg acctcgtcgt cctcatcctc
     1741 ttcatcgaga tcgtcatcgt cgtcatcgtc tttcgttttt ttggggggtt cgtcatcttc
     1801 ctcttcaggc acatgaaaat cttctacgct gatcggttca acatcgacgt cgatctcatc
     1861 gtcaatatcg tcatcctcca aatcaaggct gaagacatca ccaacctcat cgatctcatc
     1921 gtcatcaggc tccccgtcaa acccgttgat ctcattggtt tcaagcggct cgacaagctc
     1981 gatatcgttg tcggtcaaga gttcctcaat ttctactcga tattcatcgc ccaaattttc
     2041 gagcttcttc ataaggaggt ctttgctgac gcttttcgtg tcccccgctt ctttaactaa
     2101 atcgttaaaa atgttattga caatagtgtt atccagcatc tgtttccttt accctgtttc
     2161 tctatccaca ctccgaatcc gtgttggagt ttgtgcgcgt taaaaagcct tggtttgaat
     2221 caaccaagtg aacccaaagg tgtattacat cagtatgttc tctcccttcg tatactcgtc
     2281 atctttcagc acttcgtcaa tcttgggtca tgcgggccca agtcggtgac ctttttcacg
     2341 gacgtcgtta catcatacag atttatccgt gcctttacaa gagaagcaaa gctgatgcca
     2401 tcctccctgt ttcctccttc agctcctgct ttattcgggc gatttcctcc tcaatttcca
     2461 ctcgttcttc caatagctct tcaattgtcc ggctgtcgtt cccctcaagc tgcgtgatat
     2521 ccaacaagcg tttgttcgat tggcgctttt tctcccatga tcgaaggcgg attctcaaga
     2581 gactctcttt caggatcttc tgcggttcgt ttcgaaactc cgccaatcca aatgatgagg
     2641 aaaggtcgct ttttacctgg tcgctctcga gcaattgcaa gagatactca ctgcctgcaa
     2701 gggcatcctc ccgggcggcc tgctccaacg cttcatacag ttcgtgggct ttcggatcat
     2761 tcaggctttc aaatccgatc gaagcgcgag ttttcaagta cagctcgggg ttgttcgcca
     2821 cggtcagcat caggtaatag tccaaagaga tcgccgccgg attcagcgga acaaccggtt
     2881 gtgcctcccg cttgggcacg acgttcttcg gggtttgctc cttggcgtat tcctcatgca
     2941 tcgaggcttc atcgatatcc aggagatcgc tgagggtctt gatcaattcc tgccgctcga
     3001 tctcgctgtg cgtcgccctc agatagggtt tgagctgatg aaatgcacgg gccttccctt
     3061 ttgccgtgga cg
//
LOCUS       DAOPUZ010000109         3066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_32838_length_3066_cov_3.214547, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000109 DAOPUZ010000000
VERSION     DAOPUZ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32838_length_3066_cov_3.214547"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKN79_02605"
     CDS             <1..352
                     /locus_tag="PKN79_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HNZ94684.1"
                     /translation="EGIFSNAGKLSGGNQQKLIVGREMVASPQLLIAVQPTRGLDVGA
                     IEYIHSQLVAHRDKGHAVLLVSFELDEIFNLSDRIAVMHSGRIMDVVLPSETDEATVG
                     LMMTGIEAKGAGHE"
     gene            345..1433
                     /locus_tag="PKN79_02610"
     CDS             345..1433
                     /locus_tag="PKN79_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94685.1"
                     /translation="MSKIKRKESLGSNAFLMAVSAVILGLVAGAIMMAAISSNPFSGF
                     YYLFRGGLMNIERIGNTMATATTLMLVGLSVAFAFKTGLFNIGAAGQMLVGGLCATIV
                     AHKVFLPRPLFLVVLIASGLLGGAIWGVIPGLLKARFNVHEVVATIMMNWIAYWSIYY
                     FVPAYLKGPSLETESASIALTQSLRTPWLTKLFNGSYMNLGFFIAIICVILVKFILDK
                     TTLGFGLKTVGFNRHCAEYAGIKVNSNVVISMMIAGGLAGLAGLTFYTGYSRNMQIGV
                     LPAQGFDGIAVALLGASNPIGVFFSSLFFGILQSGKGFMNAMTSVPPEIADTIIATII
                     YFTATSVLFGRIWESIFKRRRKSAKEEQ"
     gene            1435..2367
                     /locus_tag="PKN79_02615"
     CDS             1435..2367
                     /locus_tag="PKN79_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94686.1"
                     /translation="MWSTLSSIFPYAIGYTMPMLMAALGGLYSERSGVTNLGLEGLMV
                     IGYFFSALAVKLTEATLGANALGLGLLVGTVAGALFSLLHAFASINLKADQVISGTAI
                     NMIAGALTVYLARTITGSGNVRIMMGIIRKNIPVLSEIPIVGPLFFTQTYWTTWICLL
                     VWLVSYFLLSKTSFGLRLRACGEHPSAVASAGVNVHLMRYIGVIISGALSGFGGSVIL
                     ITYSGEFNGTVAGLGFLSIAALIFGQWKVLGILGATFFFGIATTIANVSQVIPSLMIV
                     PPVYFKIFPYVATLLALVAFSKRSAAPLASGQPY"
     gene            2381..>3066
                     /locus_tag="PKN79_02620"
     CDS             2381..>3066
                     /locus_tag="PKN79_02620"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNZ94687.1"
                     /translation="MDNLMKKMQEAAMFITSRIGQIPVDIGMVLGSGLGDLADDLANS
                     IAISYKDIPHFPVSTVFGHKGRLIAGDLEGKRVLCMQGRFHYYEGYGMDQVVFPIQVM
                     QALGIKHLLVTNAAGGVNTSFSVGDLMLITDHIKLIADSPLRGPNPDQLGERFFDMTE
                     AYDKGLIGMAKTQARELGIAVRQGVYMFFSGPAYETPAEIRAARVLGADAVGMSTVPE
                     ALVASHMSMRT"
BASE COUNT          630 a          837 c          874 g          725 t
ORIGIN      
        1 tgaaggaatc ttctccaatg ccggcaagct cagcggggga aaccagcaaa aactcattgt
       61 cggacgggag atggtcgcaa gcccccagct gctcattgca gtgcaaccga cccggggttt
      121 ggatgtcggg gcgattgaat atattcactc ccaactggtt gcccaccgtg acaaaggcca
      181 cgccgtgctc cttgtttctt ttgaactgga tgagatcttc aatctttccg atcgaatcgc
      241 ggtgatgcac tccggcagga tcatggatgt ggtcttaccg agcgaaaccg atgaagcgac
      301 agtcggcttg atgatgacgg gaattgaagc gaaaggagcg gggcatgagt aagataaaaa
      361 gaaaagaaag tcttggcagc aatgcgtttc tcatggcggt cagcgcggta atcctcggct
      421 tggtcgcagg agcgatcatg atggcggcca tcagctccaa cccgttcagc ggattctact
      481 atctcttccg aggcgggttg atgaacatcg agcggatcgg caatacgatg gcgaccgcga
      541 cgaccctgat gctggtcggt ctttcggtgg cgtttgcgtt taaaacaggt ctgttcaata
      601 tcggagcagc cggtcagatg ctcgtcggcg gactctgcgc cacgatcgta gcgcataagg
      661 tgttccttcc ccgtcccctc tttctcgtcg tcctgatcgc aagcggcctt ctgggcggag
      721 cgatctgggg ggtgattccg ggtctcttga aagcccggtt caacgtgcac gaggtcgtcg
      781 cgacgatcat gatgaactgg atcgcctact ggtcgatcta ctatttcgtt cccgcgtatc
      841 tcaaaggacc ctctttggag actgagagcg cttcgatcgc gctcacgcaa tctcttcgca
      901 ccccatggct caccaaactc ttcaacggat catatatgaa cctcgggttc ttcatcgcga
      961 tcatctgtgt gatcctggtc aagttcatcc tcgataaaac aactctgggc ttcggcctca
     1021 agacagtcgg attcaaccgt cactgcgccg aatatgccgg aatcaaagtc aacagcaacg
     1081 tggtcatctc gatgatgatc gccggaggtc ttgccggcct tgccggtctt accttctata
     1141 ccggttattc gcgcaatatg cagatcggcg tcctgccggc tcaaggtttt gacggaatcg
     1201 ccgtggcact tctgggggca agcaatccga tcggggtatt tttcagttcc ctcttcttcg
     1261 ggatcttgca atcggggaaa gggtttatga atgcgatgac ctccgttccc cccgagatcg
     1321 ccgacacgat catcgcgacg atcatctact tcaccgctac cagcgtcctc ttcggtcgga
     1381 tctgggagtc gattttcaaa cgccgaagaa aaagtgcaaa ggaggaacag tagtatgtgg
     1441 agtaccttat cgagcatctt cccctatgca atcggctata cgatgcccat gctgatggcc
     1501 gctttgggcg gactgtacag cgagcgaagc ggtgtgacca acctgggtct ggaagggttg
     1561 atggtcatcg gatatttctt cagcgccctt gcggtcaagc ttaccgaagc gacattggga
     1621 gcgaacgccc tcggactcgg ccttttggtc gggacggtcg ccggagcgct tttcagtttg
     1681 ctccacgcat ttgcctcgat caacctcaaa gcggaccagg tgatcagcgg aactgcgatc
     1741 aacatgatcg ccggtgcgct taccgtgtat ctagcccgaa cgatcaccgg cagcggaaac
     1801 gttcggatca tgatgggaat catccgaaag aatattccgg ttctctccga aattccgatt
     1861 gtcgggccgc tgttcttcac tcagacgtac tggacgacgt ggatctgtct tctggtctgg
     1921 ctcgtttcct atttccttct ttccaagacg agtttcggcc ttaggctccg agcgtgcggc
     1981 gaacacccct cggcggtcgc ctcggcgggg gtgaacgtcc atctgatgcg ctatatcggc
     2041 gtcatcatca gcggagcgct ttccggcttc ggcggatcgg tgatcctcat cacctacagc
     2101 ggcgagttca acggaacggt ggcgggtctc ggattccttt ccatcgccgc gttgatcttc
     2161 ggacagtgga aagtgttggg aatcctggga gcgacgttct tcttcggaat cgccaccacg
     2221 atcgccaacg tcagtcaggt cattccttcc ttgatgattg ttccgccggt atacttcaag
     2281 atcttcccct atgtagcgac gctcctcgcg ttggtagcgt tctcgaagcg atcggcagca
     2341 ccgctggcaa gcggtcaacc atattgagtc ggaggaatga atggataatt tgatgaagaa
     2401 gatgcaggag gcggcgatgt tcatcaccag ccgcatcggc cagattcccg tcgatatcgg
     2461 aatggttctg ggcagcggtc tgggtgattt ggcggatgat cttgcaaaca gcatcgctat
     2521 ttcctataag gatattccgc attttcccgt ctccacggtc ttcgggcaca aaggccgtct
     2581 gatcgccggc gatctggagg gaaaacgggt gctctgcatg caggggcgct tccactacta
     2641 cgagggatat gggatggatc aggtggtctt tccgatccaa gtgatgcaag ccctcggaat
     2701 caagcatctc ttggtaacca atgcggccgg aggggtgaac acctcctttt ccgtcggaga
     2761 tctgatgctc atcaccgatc atatcaagct gatcgcggac agtccgctgc gtggaccgaa
     2821 ccccgatcag ctcggggagc gcttctttga tatgaccgaa gcgtatgata aagggttgat
     2881 cggcatggcg aaaactcagg cacgggagct tggcatcgcc gtccgacaag gggtgtacat
     2941 gttcttctcc ggtccggcgt atgagactcc tgcggagatt cgggccgccc gggtcctcgg
     3001 agcggatgcg gtcgggatgt ccaccgtgcc ggaagctctc gtcgccagcc acatgagcat
     3061 gcgcac
//
LOCUS       DAOPUZ010000110         3065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_32850_length_3065_cov_3.146512, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000110 DAOPUZ010000000
VERSION     DAOPUZ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32850_length_3065_cov_3.146512"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PKN79_02625"
     CDS             complement(<1..1022)
                     /locus_tag="PKN79_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNZ94688.1"
                     /translation="MAKIFVSGHKNPDMDSICSAYAYAALKNKVDPSNTYIPIRCGNL
                     NEATRAQFNRFGLIPPPFKRDVRTRVSKVIIESDVEVCPTDPVYRLITLYKNTARHSV
                     FPIMEGTRFVGLVTIDEISDFILKENLGSRPVYNFLVKNFEVVIPGCFIKKGNEKSIL
                     APLAVGANKFATYEKVMKGFKGPMPILVVGDRERHINKAIELNVPAIILTGIRGKTIS
                     KIDWDSYEGSVFVSETDTAETLRLLRLCVPVSELLVKKLPSLQADMLFDEARAFLANN
                     DYRELPVFSGKEYRGYVSRRSFLEKPTTKLIMVDHNENHQAITGVEEAEVVEIVDHHR
                     LGAAKTR"
     gene            1188..1634
                     /gene="rpiB"
                     /locus_tag="PKN79_02630"
     CDS             1188..1634
                     /gene="rpiB"
                     /locus_tag="PKN79_02630"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNZ94689.1"
                     /translation="MPKVVIANDHGAVELAVTLTEYLKKMGYEVNHLGTTTEDSVDYP
                     DMALAATTEFKKGGYEFGILLCGTGIGVSISANKVDGIRCALVHNTYTAQMAKRHNDA
                     NFIAFGGRVVYREEVEDILDAFIDATFEGERHQRRVDKIMALENRN"
     gene            complement(1631..2794)
                     /locus_tag="PKN79_02635"
     CDS             complement(1631..2794)
                     /locus_tag="PKN79_02635"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNZ94690.1"
                     /translation="MIEAILFDMDGVLIDSEPIILKAAMRLFADKGVTVKKEDFTPFI
                     GTGDKRYLLGVGEKYGLDLDFEVEKLVLFNHYARIAKESGPLGGVIRFITNAKRAGMK
                     IALVTSAVRMKMLLNLEAVGVEESIFDKVVTGDMVKRNKPHADIYQIASLLLGIDSKK
                     CLVVEDALNGTVAGKAAGCTVLGLTTTFGREELFESGADAVMGTLDEFDDFSTSNEFN
                     ELLRGYVGVQGGTPFGANLIADPVKEMDEGVLREAIAAALKARLNAYAPYSNYQVGSA
                     IVSHRTKGVYPGANVENSSYGATICAERSALLRAIADEGVVGIECLVVVSSDSPPAPP
                     CAQCLQVLAEFSRSDTAVHLLDVDAAEGRDGVHLVRRFDELLPHPFIFPSRRF"
     gene            complement(2794..>3065)
                     /locus_tag="PKN79_02640"
     CDS             complement(2794..>3065)
                     /locus_tag="PKN79_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HNZ94691.1"
                     /translation="LYPRKGALRIGSDADFVLFDPDHQWKIGDASIHSASGYSAYAGM
                     TVVGKALMTYLGGRLIMGDGVYLGLAGKGEFIPQHSVGRGKTVMH"
BASE COUNT          708 a          855 c          802 g          700 t
ORIGIN      
        1 cgggtcttcg ccgccccgag gcggtgatga tcgacgatct cgacgacctc ggcctcctcc
       61 accccggtga tcgcctgatg gttttcattg tggtcgacca tgatcagttt ggttgtcggc
      121 ttttccaaaa aggaacgccg cgagacatag ccgcggtatt ccttgccgct gaagaccggc
      181 agctccctgt aatcgttgtt ggcgagaaat gcccgcgctt cgtcaaagag catatccgcc
      241 tgaagcgagg gaagtttttt tacgagcagt tcactgaccg gaacgcacag gcggaggagt
      301 cgaagcgttt cggcggtgtc cgtttcgcta acgaacacgc tcccctcata ggaatcccaa
      361 tctatcttgg agattgtttt cccgcggatt ccggtgagga taatcgccgg aacgttcaat
      421 tcgatcgctt tgttgatgtg ccgctcccga tccccgacga cgagaatcgg catcggtccc
      481 ttgaagccct tcatcacctt ttcataggtc gcgaacttgt tggctccaac cgccaaaggc
      541 gcaaggatcg atttctcatt cccttttttg atgaagcatc cgggaataac gacttcaaag
      601 tttttgacga gaaagttgta gaccggccgg cttcccagat tctccttgag aataaaatca
      661 ctgatctcat cgatcgtaac caacccgaca aaacgggttc cttccatgat cgggaagacg
      721 gagtggcgtg cagtattctt atagagggtg atcaggcggt agacgggatc ggtgggacac
      781 acttcgacat ccgattcaat gatgactttg gagactcggg ttcgcacatc tcgtttaaac
      841 ggcggaggta tcaacccaaa tcgattgaat tgagcccggg ttgcctcgtt gaggtttcca
      901 caacgaatcg gaatataggt attgctcgga tcgaccttgt ttttcagcgc agcatatgca
      961 taggcgctgc agatcgaatc catatcgggg ttcttgtgtc cgctgacgaa aatctttgcc
     1021 actatccact cctgttgccc ctatcgtagc gtaatttcga ggggcgtaca actgaggcag
     1081 aatgaaaaac cccggtggaa aagcttgttg tttcaccgac caaaccctcc tcttctcatt
     1141 ggaaatactt acttgttggg gtagaatcga agcgtggagg aatcgtaatg cctaaagtag
     1201 ttattgctaa tgatcatggt gcagttgagt tggcagttac tctcacagag tatctgaaga
     1261 agatgggtta cgaagtgaac cacctcggta ccacaactga ggattcggtc gattacccgg
     1321 atatggcgct cgcagccaca acggagttca aaaaaggcgg atacgagttt ggaattcttc
     1381 tttgcggaac gggaatcggg gtatcgatca gcgcaaacaa agtcgatgga atccgctgtg
     1441 ccctcgtcca caacacctac accgcccaaa tggccaaacg gcataacgat gcgaacttca
     1501 tcgcattcgg ggggcgggtc gtctatcgcg aagaggtcga agacattctc gacgcgttca
     1561 ttgacgccac ctttgaaggt gagcgccacc aacgacgggt cgacaagatc atggcgcttg
     1621 aaaaccggaa ttaaaaccgt cttgaaggaa agatgaaggg gtgcggaagg agctcgtcaa
     1681 agcgacggac cagatgcacc ccgtctcttc cttcagccgc atcgacatcc agaagatgaa
     1741 cggcggtatc actgcggctg aattcagcga gaacctgtaa acattgggcg cacggcggtg
     1801 ccggaggaga atcggagctg accaccacca gacactcgat tccgacgaca ccttcatcgg
     1861 caatcgccct caacagggcg ctgcgctcgg cgcagatcgt cgccccatag gaggagttct
     1921 ccacgttggc tcccggatac acgcccttgg ttcgatggct tacgatcgca ctgcccacct
     1981 gatagttcga gtacggcgca tacgcattga ggcgagcctt caacgcggcg gcgatcgcct
     2041 ccctgagaac tccctcatcc atctctttga cgggatcggc gataagattg gctccaaacg
     2101 gagtgccgcc ttgaacgccg acataccctc gaagcagctc attgaactca ttcgaggtgg
     2161 aaaaatcatc aaactcatcg agggtgccca tcaccgcatc cgccccgctc tcaaagagct
     2221 cttcccgccc gaaggtggtg gtcaagccaa gaacggtgca gccggcggct tttccggcga
     2281 ccgttccatt gagggcatct tcgacgacca ggcacttctt tgagtcgatg cccaagagga
     2341 gcgaggcgat ttgataaata tctgcatgag gcttgttgcg tttcaccatg tccccggtga
     2401 cgaccttgtc aaaaatcgat tcctcgaccc cgaccgcttc caggttgagc agcatcttca
     2461 tccggactgc gctggtgaca agggcgatct tcatgcccgc ccgtttggcg tttgtgatga
     2521 agcggatgac tccccccagc ggtccgcttt cttttgcgat ccgggcgtag tggttgaaga
     2581 gaacaagctt ttccacctcg aagtccagat caagtccata cttttccccc acccccaaga
     2641 gataccgctt atcaccggtg ccgatgaaag gagtgaaatc ttccttcttg acggtcacac
     2701 ctttatcggc aaagagccgc atcgccgctt tgagaataat cggctcggag tcgatcagca
     2761 ctccgtccat gtcaaacaag atcgcttcaa tcatcagtgc atcaccgttt ttcctcgtcc
     2821 caccgagtgt tggggaatga attcaccttt ccctgccaac ccgaggtaca ccccatcgcc
     2881 catgatgagc ctgccgccaa ggtaggtcat cagcgctttc ccgacaacgg tcatgcctgc
     2941 atatgcgctg tatccgcttg ccgagtgaat ggaggcatca ccgatcttcc actgatggtc
     3001 gggatcgaag aggacgaagt ccgcgtcgct tccgattcgc aaagctcctt ttcgcggata
     3061 gaggc
//
LOCUS       DAOPUZ010000111         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_32969_length_3058_cov_3.269730, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000111 DAOPUZ010000000
VERSION     DAOPUZ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32969_length_3058_cov_3.269730"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PKN79_02645"
     CDS             complement(<1..244)
                     /locus_tag="PKN79_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94692.1"
                     /translation="MITIRKLKSLKEDTRLRKATLLLKELSRLGEIDSSYVKDLLQII
                     KESRAGGDKRVVGLIDRIGGQEGRARAFSMEDLHYRL"
     gene            complement(213..929)
                     /gene="rsmI"
                     /locus_tag="PKN79_02650"
     CDS             complement(213..929)
                     /gene="rsmI"
                     /locus_tag="PKN79_02650"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNZ94693.1"
                     /translation="MSTLYIVATPIGNLADMTYRAVEVLTNVDIIACEDTRHTQNLLN
                     HYKISKRLIATHAHNEDNSAKGIIGLLEEGKDVAFVSDAGTPGISDPGSRLVERVRSS
                     GFDVVPIPGASAFTTLVSVAGYIGRSIHFEGFLSPKSGRRKKRLEELLQRDESFVFYE
                     SPFRVVKVLEELSTLDPGRRVVAGREMTKKFEEFLTGSVEEVLDSLKGRQTVKGEFAL
                     LVAPKGAGASDDYDQETEEP"
     gene            1140..1820
                     /locus_tag="PKN79_02655"
     CDS             1140..1820
                     /locus_tag="PKN79_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNZ94694.1"
                     /translation="MQFFKRVRDIVSSNVNSALDKFEDPQKMIALMVTNLEELQTKTR
                     SSIAGKKAELIALNHRVKEAQDAVNRWAERAKLAVANGKEDLAREALVEKRKAQEHHD
                     QLAEQITNLEGIIAAQTTQLAQIGDKLKEVKDKQQILVQRSISARERRQVAAVLRESD
                     SSNIASKFSELEAKIERMEAEAEMAAYSGSTSVLEQFTQMETDEAIEAELAELKKSSA
                     KKAPAKTE"
     gene            complement(1874..2851)
                     /locus_tag="PKN79_02660"
     CDS             complement(1874..2851)
                     /locus_tag="PKN79_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNZ94695.1"
                     /translation="MAAQERYSQQPLGESEVFLDFQAKLSRVAAINRPVLLVGERGSG
                     KEIAARRLHYLSPRWDKNLVTVNCAALPPSLIETELFGYEQGAFTGAQKTRKGRFEEA
                     EGGTLFLDEIGLIPLEVQEKILRVVEYGTYERVGSSVTHEVNVRIIGATNADLPALCE
                     EGKFKQDLLDRLSFEVLFLPPLRERGEDILTLAYFFASKMAQECDREGMVTFTDEVID
                     KLMAYRWPGNVRELKNVIERAVYRSEDNVITELQFDPFANPFEEKTGQSKEAELVLGN
                     FEADRIEFEIRYLAEALKQGEGNQKEAAKLLGLTYDQFRGLYRKYQDRL"
BASE COUNT          699 a          839 c          847 g          673 t
ORIGIN      
        1 ggagccggta gtgcaggtcc tccatggaaa acgctctggc tcttccctcc tgcccgccaa
       61 tgcgatctat aagcccaaca acccgtttat ccccacccgc ccttgactct ttgatgatct
      121 gaagaaggtc cttcacatat gaggaatcaa tctcaccgag ccggctgagt tcctttaaaa
      181 gaagcgtcgc tttgcgcagg cgggtgtctt ccttaaggct cttcagtttc ctgatcgtaa
      241 tcatcgctcg ctcctgcgcc tttcggcgcc acgagaaggg cgaactcccc cttcacggtt
      301 tgtcgtcctt tcagtgaatc aagcacctct tcgaccgatc cggtcagaaa ctcttcaaac
      361 ttcttggtca tctcccgccc cgccacgacc cggcggccgg gatccaaggt ggaaagctcc
      421 tcaagaacct tcaccacccg aaagggggat tcatagaaca cgaagctttc atcccgctga
      481 agcagctctt ccaggcgttt cttccgtctt ccgcttttgg gagaaagaaa cccctcgaag
      541 tggatgcttc gaccgatgta accggccacg ctcaccaagg tggtgaaggc gctggcgccg
      601 gggatcggca ccacgtcaaa gccggatgag cggacgcgtt caacgaggcg gctgccggga
      661 tcgctgatgc cgggagtccc ggcatcggag acaaaggcga catccttgcc ctcttcaagc
      721 aggccgatga tccctttggc ggagttgtct tcattgtggg cgtgggtcgc gatcaggcgc
      781 ttggaaatct tgtagtgatt cagcaggttc tgcgtatgcc gggtgtcttc acaggcgatg
      841 atgtcgacgt tcgtcaacac ttcgacggct cggtaggtca tgtcggccaa gtttccgatc
      901 ggggtcgcca cgatatagag ggtgctcatt gtttcagtct acacctccgg ggctgaggga
      961 acaagccaac ccatgatgat catatggtgg tatttataac cacgggcatt ggtatattta
     1021 accaaaaagg tggtcttata gaaaaataca cctctgaatc actcatttat tgctcttttt
     1081 cacgagttgg cacgccttct gcattaggag agataccaaa gaagaacagg aggcatgcaa
     1141 tgcaattttt taaacgagtc agagatatcg tcagctccaa cgtgaacagc gctttagata
     1201 agtttgagga tccgcagaag atgatcgccc tgatggtcac caatctggag gagcttcaga
     1261 cgaagacccg ctcctcgatc gcgggaaaga aagcggagct tatcgcgctg aaccaccggg
     1321 tgaaggaagc acaggatgcg gtgaatcgct gggcggagcg cgccaagctt gcggtggcga
     1381 acgggaagga agaccttgcc cgcgaggctc tggtggagaa gcggaaagct caggagcatc
     1441 acgatcagct agccgagcag atcaccaatc tggaagggat catcgccgcc cagaccaccc
     1501 agcttgccca gatcggcgac aagctgaagg aagtgaagga caagcagcag attctcgtcc
     1561 agcgctcgat cagcgcccgg gagcgtcgtc aggtggctgc ggtcctaagg gaaagcgact
     1621 catcgaacat tgcgtcgaag ttcagcgaac ttgaggcgaa gatcgaacga atggaagcgg
     1681 aagcggaaat ggccgcctac agcggttcca catcggtcct tgagcagttc actcagatgg
     1741 agaccgacga agcgatcgaa gcggagcttg ccgaactgaa gaagagctcg gcaaagaagg
     1801 ctccggcgaa aaccgagtaa gcgtcagacg tatttctcca tggacgggca tcacgcgatg
     1861 cccgtttctc tttctatagg cgatcctgat actttcgata cagtccccga aactgatcgt
     1921 aggtgagccc aaggagcttc gccgcttcct tctgattccc ttccccctgc ttgagcgctt
     1981 cggcaaggta gcggatctcg aactcgatcc gatccgcttc aaagttcccc aagacaagtt
     2041 cagcctcttt cgactgtccg gttttttcct cgaaggggtt ggcgaaggga tcgaactgaa
     2101 gttcggtgat gacgttgtcc tcgctgcggt agaccgcccg ttcgatcacg ttcttcagtt
     2161 cccggacgtt tcccggccac cggtacgcca tgagcttgtc gatcacctcg tcggtgaagg
     2221 tgaccatccc ttcccggtcg cactcctgcg ccatcttcga agcgaagaag taggcaaggg
     2281 tgaggatgtc ctcaccccgc tcccgcaagg ggggcagaaa gaggacttcg aaggaaagac
     2341 ggtccaggag gtcctgcttg aacttcccct cttcgcacag ggcgggaaga tccgcgttcg
     2401 tcgccccgat gatccggacg ttcacttcgt gggtaacgga ggagcctacc cgttcatatg
     2461 ttccatattc aacgacccgg aggatcttct cctgaacctc cagagggatg aggccgatct
     2521 catcgaggaa gagggttccc ccttccgctt cttcaaagcg gcctttacgg gttttctggg
     2581 ctccggtgaa cgctccctgt tcgtagccga acagttccgt ttcgatcagg gagggaggaa
     2641 gggcggcgca gttcaccgta acgaggttct tgtcccagcg gggggagagg tagtggagcc
     2701 ttcgagcggc gatctccttt ccgcttcccc gttcgccgac aagcagaacc gggcggttta
     2761 tcgcagctac gcggctgagc ttcgcctgaa aatcgagaaa cacctcgctt tctccaaggg
     2821 gttgttggga atatcgttct tgagccgcca tgaccctgtt cctttggtgt aatttaccat
     2881 ccttggcata taaaaccaca attaccatag cacagggagg taaaaatcgg aagtacacgc
     2941 tcaaaaaggg cggtttcagg gttggcacgc tttatgcata ccaattacta taaaaatgaa
     3001 caaggagcgt aatcatggga gtattttcaa gatttttaga tattgtaaat gccaacat
//
LOCUS       DAOPUZ010000112         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_33037_length_3054_cov_3.608203, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000112 DAOPUZ010000000
VERSION     DAOPUZ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33037_length_3054_cov_3.608203"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2154
                     /locus_tag="PKN79_02665"
     CDS             <1..2154
                     /locus_tag="PKN79_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ94696.1"
                     /translation="ALQAIRGNTRIDSEDPESRYEALEKYCKDLTLLARSGKLDPVIG
                     RDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEGLARRIAGGDVPESLKNKRLLSL
                     DLGALIAGAKFRGEFEERLKAVVKEVTTSEGAIILFIDELHTIVGAGAAEGSTDASNL
                     IKPALSRGELHAIGATTLDEYRKYIEADKALERRFQPVYTSEPSVESTIAILRGLQER
                     YEVHHGVRIKDEALVAAAVLSNRYITNRFLPDKAIDLIDEAASQLKMEIESQPEQLDQ
                     IERKILQLNIEEQALKRESDKASKKRLEKLTEERNTLQSERDVLHTQWEEERASIGRI
                     RESKARLEQLKSEEENAEREGDLAKAAQIKHGEIPALTREIQTWTEALKRENGNQLLR
                     EEVSEDDIAKIVSNWTGIPVAKMKSSEAEKYLKLESILKEEVIGQEEAIQAVSAAIRR
                     NKAGIGDEKRPLGSFLFAGPTGVGKTLLAKVLAQFLFDDEKALTRIDMSEYMEKFSVS
                     RLIGAPPGYVGYDQGGQLTEVVRRRPYSVILFDEIEKAHPDVFNVLLQLLDDGRLTDG
                     QGRVVDFTNAVIIMTSNLGSQQLLGAATHEEGAALVNEVIHSAFKPEFINRLDEIIVF
                     NRLDSDAVHRIIALQLKTLANRLKGRGYSLIWDEKLEDAIFEAGFDPLFGARPIKRAI
                     QRLVENPLSLKILEGAFHEGDSISLSWDGSAVVIS"
     gene            complement(2134..2727)
                     /locus_tag="PKN79_02670"
     CDS             complement(2134..2727)
                     /locus_tag="PKN79_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNZ94697.1"
                     /translation="MEGSRTTQNLLLGAGGATRSGGEKLFWIYENRPIIHHSVANSLR
                     ANLPTILVLGYRHAEIRSLIGDLVKDPLLTIVINDNWQAGQGSSTKVGARLLDRKKRF
                     FISLGDMPLITPSHYRFLERFLEPVVRPVVNGRVGHPVLHDPFLIDEILKQPDSFTMR
                     ELLSAYEMKKIAVDDPAYIRDIDTLQEYRTLLGNHYG"
     gene            2754..>3054
                     /locus_tag="PKN79_02675"
     CDS             2754..>3054
                     /locus_tag="PKN79_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HNZ94698.1"
                     /translation="MIATYFNALTIVIGSLIGLLVKSRLKPSFEEIIFTSSGLITLIL
                     GFSMAMATSSYLLMLFALALGGALGYWLKIEDRILALGTRMESVTSRGASDGEQAR"
BASE COUNT          700 a          831 c          948 g          575 t
ORIGIN      
        1 gcccttcagg cgatccgcgg caatacccgc atcgacagcg aagatccgga gagtcgatat
       61 gaagcgctcg agaagtattg caaggatctg acactgcttg cccgaagcgg aaagctcgat
      121 ccggtaatcg gacgggatga ggagatccgg agggtgatgc aggtcctcag ccggaggacg
      181 aagaacaacc cggtcctcat cggggagccc ggggtcggga agacggcgat cgttgaaggt
      241 ttggctcgaa ggatcgccgg cggcgacgtg cccgagtcgc tgaagaacaa gcgtctgctc
      301 agcttggatc tgggcgccct gatcgccggg gcgaagttcc ggggcgagtt cgaagagcgc
      361 ctcaaggcgg tcgtcaagga ggtcaccacc agcgaagggg cgatcatcct cttcatcgac
      421 gagctgcata cgatcgtggg agcgggggcc gccgaaggct cgaccgatgc ctctaacctg
      481 atcaagcccg ccctcagccg gggggagctc catgcgatcg gggcgacgac cttggatgaa
      541 taccgaaaat acatcgaagc tgacaaagcc ctcgagcgcc ggttccagcc ggtatacacc
      601 tccgaaccga gcgtcgaatc gacgatcgcg attcttcgcg gcctgcagga gcgctacgaa
      661 gtccaccacg gagtgcggat taaggacgaa gcgcttgtcg cagcggcggt gctgagcaat
      721 cgctacatca ccaaccgctt ccttcccgac aaggcgatcg acctgatcga cgaggcggcg
      781 agccagctga agatggagat cgaaagccag ccggaacagc tcgatcagat cgagcggaag
      841 attctccagc tgaacatcga agagcaggcg ctgaagcggg aaagcgacaa ggcctcgaag
      901 aagcgcctcg agaagctcac cgaagaacgg aacactcttc aaagtgagcg ggacgtcctg
      961 cacacccaat gggaggagga gcgcgcttcg atcggtcgga tccgggaaag caaggctcgg
     1021 ctcgaacagc tgaagagcga ggaggagaac gccgaacgcg agggagacct cgccaaagcg
     1081 gcgcagatca agcacggcga gatccccgcg ttgacgcggg agattcagac gtggacggaa
     1141 gcgctgaagc gggaaaacgg aaatcagctt ctccgtgaag aggtcagcga agacgacatc
     1201 gccaagatcg tctccaactg gacggggatt cccgtcgcca agatgaagag cagcgaggcc
     1261 gaaaagtacc tcaagcttga aagcatcctc aaggaagagg tgatcggcca agaggaagcg
     1321 attcaggcgg tcagcgcggc gatccgccgg aacaaagcgg gaatcggcga cgagaagcgg
     1381 ccgttgggct cgttcctttt tgccggtccc accggagtgg gaaagacgct gcttgccaaa
     1441 gtgctggctc agttcctctt tgatgacgag aaagcgctta cgcgaatcga catgagcgaa
     1501 tatatggaaa agttttccgt ctcccgcctg atcggcgccc cgccggggta tgtcggctat
     1561 gatcagggcg gccagcttac cgaagtggtg cgaaggcggc cgtattcggt catcctcttc
     1621 gacgagatcg agaaagccca cccggatgtg ttcaacgtgc tgcttcagct tctggatgac
     1681 ggccgtctta ccgacggtca gggcagagtg gtggacttca ccaacgccgt gatcatcatg
     1741 acgagcaacc tcggcagcca acagctgctc ggggcggcga cccatgaaga gggggcggcc
     1801 ttggtaaacg aggtgattca ctccgcgttc aagccggagt tcatcaaccg ccttgatgag
     1861 atcatcgtct tcaaccgtct ggacagcgat gccgtgcatc ggatcattgc cttgcagctc
     1921 aaaacgcttg caaaccggct caaggggcgg ggatattcgc tcatctggga tgaaaagctc
     1981 gaagatgcga tcttcgaagc ggggttcgat ccgctcttcg gagctcgtcc gatcaagcgg
     2041 gcgatccagc ggcttgtcga aaaccccttg agcctgaaga ttctggaagg agctttccat
     2101 gaaggggatt cgatcagcct ttcgtgggac ggttcagccg tagtgatttc ctaagagggt
     2161 ccgatattct tgcagcgtgt cgatgtcccg gatatacgcc gggtcgtcga ccgctatttt
     2221 tttcatttca taggcgctca gcagttccct catcgtgaag gaatcgggtt gtttcaggat
     2281 ttcatcgatg agaaacgggt catgcaggac gggatgaccg accctcccgt tcacgaccgg
     2341 tcggacgacc ggttccaaaa accgttcgag gaaacggtag tggctcggag tgatgagggg
     2401 catgtctcca agcgagatga aaaatcgctt ttttcgatcg aggagacggg ctccgacctt
     2461 cgtcgaggag ccctgacccg cttgccagtt gtcattgatg acaatggtca aaagaggatc
     2521 tttgacgaga tctccgatga gcgagcgaat ctcggcgtgc cgatagccga gcaccagaat
     2581 ggtgggaaga ttcgccctca gcgaattggc gacactgtgg tggatgatcg gtctgttctc
     2641 atatatccaa aagagcttct cccctccgct gcgcgtagcg cccccggcgc ccagaagaag
     2701 gttttgggtc gttcttgagc cttccataca atctgagtat actccaaatc attatgatag
     2761 ccacctattt caacgccctg acgatcgtca tcggctccct gatcggtctg ctcgtgaaaa
     2821 gcaggctgaa accttccttt gaagagatca tcttcacctc cagcggtttg atcaccctca
     2881 tcctcggatt ttccatggcg atggcgacat catcctatct tttgatgctg tttgcccttg
     2941 ccctcggcgg agcgttgggg tattggttga agatcgagga tcggattctg gccctcggaa
     3001 cccggatgga gagcgtgacg agccgagggg cgtccgacgg cgaacaggcc agga
//
LOCUS       DAOPUZ010000113         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_33081_length_3051_cov_3.605808, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000113 DAOPUZ010000000
VERSION     DAOPUZ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33081_length_3051_cov_3.605808"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..2142)
                     /locus_tag="PKN79_02680"
                     /pseudo
     CDS             complement(52..2142)
                     /locus_tag="PKN79_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270529.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(2207..2728)
                     /gene="pth"
                     /locus_tag="PKN79_02685"
     CDS             complement(2207..2728)
                     /gene="pth"
                     /locus_tag="PKN79_02685"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNZ94699.1"
                     /translation="MMKLIVFLGNPGKIYEPTRHNVGWMVAKEAYGSITWSEKFSAKI
                     AKDGPRHLLKPLTCMNESGRSVGLASSYFGIKADEILVVSDDLELAFGTIRLQQGGGL
                     KGHNGLKSIQSALGSGDFWRLRIGIGRPIHGTVSSFVLSRFTPEEEISLPLILQEAAK
                     LLHDGTAMERVLF"
     gene            2792..>3051
                     /locus_tag="PKN79_02690"
     CDS             2792..>3051
                     /locus_tag="PKN79_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94700.1"
                     /translation="MYALVFFVPTTQLEEVKNALFAKGAGRLGSYEQCSFQVRGEGQF
                     RPLAGSSPFLRGEGVLEKAEERRAGLEQLRRILVLAGGKPLL"
BASE COUNT          626 a          773 c          923 g          729 t
ORIGIN      
        1 aggggcgttt gcgaacggct tccagacctt tcatcacctt gatgttactt gcagagggaa
       61 gattctccgg tagtgttcat cgacgaggtg ttgattggcc gtctcgtccc agtaggcccg
      121 gcgatactcc atcccgtatt tttccctgaa tccctcgacc tgaccgtgac cgaacatcgg
      181 aaggcccggc atcgttgcaa ggagggtgca cactccaaaa tacttgtcgc cgtcgccgaa
      241 ttgggcgatg gcggtctcct cgtcggggtt gttcatgaag ttcacgaagc gcttgaggat
      301 ttccggttca tagatgaggg tgtttttgat cgtatcccgg tattttttat tctcctggtt
      361 tttgagcatg ttcatgaacg cgctgttgta cacccggtgc attcccaagg ttcggacgaa
      421 gtagccctcc atcatccaga acgcttcggc aagcaggagg gtgtcaggca attcctcatt
      481 gatccggtcg acgacctccc gccaaaactc attggggatc aggcggtgga acgtctcgtc
      541 atcgagtgaa tggggaactc ggccggcgat gtcgccgcct ctgccggggc ggggatacca
      601 gagccgttga atgtgcttct tcgccaacgt catcgccgca tcgaagcgga tgatcgggaa
      661 gttcatcgcg acgtgcttga tctgctgata caccatctcc cgggtcttcg ggttgaggaa
      721 atcgagctga gcggtgtcgt tccagggcat cgatgttcca tcgttgccgt ggaagatata
      781 gtgtgtctca ccggtattga ggtctcttct ttggaacgtg accgctgcgt cgctgcggtc
      841 gtagtagtgg tcttccagct tgatctcaac gttgggattg gaggagagat tcggtccgtt
      901 gaacgtgtag gaggggaagg gcggatgtct ctgccagatg aagaggtcgg ggttgctcat
      961 cacccagtcg ctgtcgatgc ccgtgtggtt ggggaccatg tcgctggcaa ggcggactcc
     1021 atacttggaa caggtatggc gaagggaatc caaagcgccc catcctccga tgagggaagc
     1081 gatctcgtag ttcctcaagg agtatgccga agcttcggca tcggggttgc ctgtaaggtt
     1141 cttgatcatc ttgctggcgc ttgagcgctc ccagatcccg atcagccaca gagcggtaaa
     1201 ccctcgttgg gccaacagct cgatctcccg ttcggggatc ttgtcgagcg tatcgatcgg
     1261 atagccgtac tccttcgtca gttgatcgag ccataccaac gtggatttcg cgatcattac
     1321 gacattgggc atccagtttc gatcggcggt gaacgcttca tactcatgat cgaagaggga
     1381 gtagtcgatc accggcatcg gaggagcggg accgccgggt gggaaatggg gcttttcctc
     1441 ctcttggacg tagtcgatcg cccgcagcag gaggcgtttg agctcgctgt cgatcatgtc
     1501 cgcccagtgc tcgaggatgt atcgaatctg gtcgaggagc gaatcgggat agaggcgggc
     1561 gggccgggta aggaacgaaa agagatcatc atcttttgcc gcggtgcgct gttgagcata
     1621 cccgcccatc agggcggtga gggcctggtg ggctttgggg aagaacagtc cctccggctt
     1681 caacagaggt ttggaggcgg cgatgagggc ggggttttcc tccatcacct gatggatgaa
     1741 gtagccgcga agggattcga ggtagtaaaa ctccaccgga tacgatttcg agacaagcaa
     1801 gggcgagggg aactccttca tgtagaatgt caatacttcc tcgatatcgt ggtttttccc
     1861 caccaaggtt ccgagccggc tgaagaagtc cggttcatgt gtgttgaggt agttggaaag
     1921 aacgctttgg tagagctggt gaagcaccgc caccgcgtga agctttccgc tggaaagata
     1981 atcgttgccg cccttgcttc gaaccttctc attgtaccgg tatgcaagct cggtggcttc
     2041 ctgatagagc tcgttcaagt gagcgtccga gggaaagagc gggggaaaaa aatcaagctc
     2101 ggtacgggtt cgtacggaga ttctgaaatc gcgtggaatc aagaggcacc tcccaaacca
     2161 atggtaatgt gagtatatca cgccggggtg atgttgtaac cccttcttaa aagagtactc
     2221 gctccatggc tgttccgtca tgaaggagct ttgccgcttc ttgcaaaatc aggggaagag
     2281 agatctcctc ctcaggggtg aaccgggaaa ggacgaatga ggacaccgtc ccgtggatcg
     2341 gtcggccgat tccgattctc agccgccaga aatcaccgga gcccaaggcg ctttggatcg
     2401 acttcaatcc gttgtgtcct ttcaaccctc ccccttgctg gaggcggatc gttccaaatg
     2461 caagttccag atcatccgag accacgagaa tttcatcggc cttgattccg aagtaggagc
     2521 ttgccagtcc gaccgaccgg ccgctttcat tcatgcaggt caacggtttc aataaatgcc
     2581 tcggtccgtc tttggcgatt tttgctgaaa acttctcgct ccaggtgatc gaaccgtacg
     2641 cctcctttgc aaccatccac ccgacgttgt gccgggtcgg ctcgtagatt tttccggggt
     2701 tgccgagaaa gacgatcagt ttcatcatat cggaagtgta aaagaggggc tcctccatga
     2761 caagatggat gctctttcgt atgatgtgtc catgtatgct cttgtattct ttgttccgac
     2821 gacccagctt gaagaggtga agaacgccct gtttgcgaag ggggccggta gattgggctc
     2881 ttatgaacag tgcagcttcc aagtcagggg cgaggggcaa ttcagacctt tggcgggaag
     2941 cagcccgttt ctccgaggtg aaggcgtgct tgagaaggcg gaggagcggc gcgcaggtct
     3001 ggagcagctg cggcgcatcc tcgtgcttgc gggcggcaaa cctctgctgt t
//
LOCUS       DAOPUZ010000114         3047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_33142_length_3047_cov_3.116979, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000114 DAOPUZ010000000
VERSION     DAOPUZ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33142_length_3047_cov_3.116979"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PKN79_02695"
     CDS             <1..602
                     /locus_tag="PKN79_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ94701.1"
                     /translation="IFAADKVTGGEVRIHGKRVKIKTPLHAIKHGIGYLPEDRKGDGI
                     IEDLSVRDNIILTLQVLKGLFKPFSLKEAEKFAEEYIKKLNIKTPTSNTPIRSLSGGN
                     QQKVILARWLLTNLEYLILDEPTRGIDVGTKVEIQKLVIRLASGGMSLTFISSEIDEM
                     LRTCSRLIVMKDREIVGELRGTDLTENDVMHMIAQGGKS"
     gene            614..1630
                     /locus_tag="PKN79_02700"
     CDS             614..1630
                     /locus_tag="PKN79_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94702.1"
                     /translation="MKNVSHLVLPISVMVLLIIINLVKGADYFTITMINGALYGNIPN
                     ILFGASELVILSIGMTLVTAASRGQDISIGESATISSAVFVWFVLRSGEATPVTIIVG
                     FLLSCVVGLGLGAFNGILVSIFKVQPMVASLILFTGGRSIAFMIDGKLSPILANDISN
                     RIGTVIPGIPIQTPVILTAVFIALFAVLLKTTTLRLYVETVGINPNAARLNGINPKKV
                     IFLTFLIMGFCSAVAGFIAVNKAGRHDSVNLLKNIMMDAILAVAIGGNSLGGGTFSIT
                     GSIIGAYTIEMLNRTLLRLEIEPAMIKVFKAIFIVILMVVASPVVRDFIKKVLDRNRT
                     RKEE"
     gene            1632..2711
                     /locus_tag="PKN79_02705"
     CDS             1632..2711
                     /locus_tag="PKN79_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608103.1"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HNZ94703.1"
                     /translation="MASEKAIKQKARLSNSNLLFLIASVIFVLMYLFAIISYPGSFLQ
                     FQTFFDLFNLNAPLIIMTFGLCIVMIGGGIDISIGSVCGLVTMACAVYLEKRSGSIAG
                     AILLSMGIGILFGILHGYLIAYLEIQPFIITLSGLFLAQGLLTTLHKDPLNVTMSAFV
                     NLRDFNIVITWLGTRNRLGVFIPCQIKPGTIIVAVLLVIFASLMKWSRFGRNVYAVGG
                     NSHSAMMLGINVKRTKFITYVISGLTAGIAGFVYIMTTGAGNIGNAAGAEMKAIASAI
                     MGGTLLNGGVGNLLGAPIGTLTLLVINELIRAAGVQSNFQAIVSGLLLYLFIILQSII
                     MSLRDRRRFTVALPPWLRLSGKGQA"
     gene            complement(2721..2978)
                     /locus_tag="PKN79_02710"
     CDS             complement(2721..2978)
                     /locus_tag="PKN79_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ94704.1"
                     /translation="MASIVHESPNHFSTIFSQETGETFIEYLTGVRINKSKELLLTTA
                     LRSADIAYEVGFGDSHYFSFIFKKHTGISPREFRTGNKAQT"
BASE COUNT          693 a          821 c          827 g          706 t
ORIGIN      
        1 ccattttcgc cgccgacaag gtgaccggcg gtgaagtcag gatccatggc aagagggtga
       61 agatcaaaac gcccctgcac gccataaaac atgggatagg ctatctgccg gaggatcgca
      121 agggggacgg aatcatcgag gacttgtcgg ttcgcgacaa catcatcctc accctgcagg
      181 ttctgaaagg cctgttcaag cccttctcgt tgaaagaggc ggaaaagttc gccgaagagt
      241 acatcaaaaa gctgaacatc aagactccga cctccaacac cccgatacga tccctttccg
      301 gaggcaacca gcagaaggtc attctcgccc gctggctgct tacgaacctc gaatacctga
      361 ttctcgatga accgacccgg ggaatcgatg tcggaaccaa ggtcgagatc cagaagcttg
      421 tgatcaggct tgcttccggc ggaatgagcc tgaccttcat ctcttccgaa atcgacgaga
      481 tgctccgaac ctgctcccga ttgatcgtca tgaaggaccg tgaaatcgta ggagaactca
      541 ggggaacgga tctgacggag aacgatgtga tgcacatgat cgcgcagggg ggaaagagct
      601 gatgcgaaaa ttgatgaaaa acgtttccca cttggttctt cccatctccg tcatggtgct
      661 gctcatcatc atcaatctgg tgaaaggcgc tgattatttc accatcacga tgataaacgg
      721 agcgctctac ggcaatattc cgaatatcct gttcggggcc tccgagctgg tcatcctgtc
      781 gatcggcatg acgctggtga ccgcggcatc ccgggggcag gacatcagca taggggagag
      841 cgccacgatc tcttccgccg tattcgtctg gttcgtcctt cgatccggcg aggccactcc
      901 ggttaccatc atcgtcggct tcctgctcag ctgcgtcgtg ggtttggggc ttggcgcttt
      961 caacggcatc ctcgtttcga tcttcaaggt tcagccgatg gtcgcatccc tcatcctgtt
     1021 taccggcgga aggtcgatag ccttcatgat cgacggcaaa ctgtctccca ttctggccaa
     1081 cgacatatcg aacaggatag gcaccgtgat cccgggaata ccgatccaga ctccggtgat
     1141 cctcactgcc gtcttcatcg ctctctttgc ggtgctgctg aagaccacga cgctcaggct
     1201 ctatgtggaa accgtcggca tcaacccgaa tgccgcccgg ctgaacggca tcaacccgaa
     1261 gaaagtcatc tttctaacct tcctgatcat gggattctgt tcagcggttg cagggttcat
     1321 cgcagtgaac aaggcggggc ggcacgacag cgtcaacctg cttaaaaaca tcatgatgga
     1381 tgcgatcctc gccgtggcga tcggcgggaa ctcactggga ggtggaacgt tcagcatcac
     1441 cggctcgatc atcggagcgt acacgatcga gatgctgaac aggacgctcc tcaggctgga
     1501 gatcgaacct gcgatgatca aggtcttcaa ggcgatcttc atcgtcattc tcatggtcgt
     1561 cgcttctccc gtggtcaggg atttcattaa gaaggttctg gacaggaatc gaacaaggaa
     1621 ggaggaatag aatggcatcc gaaaaagcga tcaaacaaaa agccagactg tcgaattcga
     1681 accttctgtt cctcatcgct tcggtcatat tcgtgctgat gtacctgttc gcgatcatca
     1741 gctatccggg cagcttcctg cagttccaga cgtttttcga cttgttcaac ctcaacgctc
     1801 cgcttatcat catgaccttc ggtctgtgca tcgtcatgat cggcgggggc atcgacatct
     1861 ccatcggctc ggtctgcgga ttggtgacga tggcctgcgc ggtctatctg gagaaaagat
     1921 cggggagcat cgcaggagcg attcttcttt cgatgggcat cggcatcctg ttcggaattc
     1981 tccacgggta tctcattgca tatcttgaga tccagccgtt catcatcacg ctttcaggct
     2041 tgtttctggc gcagggactc ctcacgaccc tccataagga tccgctcaac gtgacgatgt
     2101 ccgcattcgt gaaccttcgg gatttcaaca tcgtgatcac atggctcggc acgagaaaca
     2161 gactgggggt cttcatcccg tgccagataa aacccggtac gatcatcgtc gcggttcttc
     2221 tcgtcatctt cgcatccctg atgaaatggt cccgcttcgg gcgcaacgtc tatgccgtcg
     2281 gtggaaacag tcacagcgcg atgatgctcg gcatcaacgt caagcggacg aaattcatca
     2341 catacgtgat cagcggattg acggccggca tcgccgggtt cgtgtacatc atgacgacgg
     2401 gggccggtaa tatcggcaat gcggcagggg ccgaaatgaa agcgatcgct tcagccatca
     2461 tgggtggtac gctgctcaac ggcggggtag gaaacctgct gggggcgccg ataggcacgt
     2521 tgacgcttct ggtgatcaac gaactgatcc gtgcagcggg agtccaatcc aactttcagg
     2581 cgatcgtaag cggcctgttg ttgtatcttt tcatcatcct gcaaagcatc atcatgtcac
     2641 tccgcgacag gagaaggttc actgtcgcgc tcccgccgtg gctacggctt tcgggcaagg
     2701 ggcaagcata gccgctgttt tcaggtttgg gctttgtttc cggttctgaa ttcccggggg
     2761 gagataccgg tgtgtttctt gaagatgaag ctgaaatagt gggaatctcc aaatcccact
     2821 tcataggcga tatccgcgct tctgagagct gtcgttaaca acagctcttt tgatttattg
     2881 atccggaccc cggtaagata ttcgatgaag gtttcaccgg tttcctgaga aaaaatcgtg
     2941 ctgaaatgat tcgggctttc atggacgatt gatgccaccg catgcaggga aatgtccaga
     3001 tctgcgggga gatactatga aatgatccag aaagcgaagc agcatat
//
LOCUS       DAOPUZ010000115         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_33269_length_3039_cov_2.276475, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000115 DAOPUZ010000000
VERSION     DAOPUZ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33269_length_3039_cov_2.276475"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /gene="rsgA"
                     /locus_tag="PKN79_02715"
     CDS             <1..676
                     /gene="rsgA"
                     /locus_tag="PKN79_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNZ94705.1"
                     /translation="ANPPFRPRFIDRVIASAIDVEVIILLNKCDLEMKEEDAFRFEHY
                     GALGFQTAAVSAETGENLDRLVQIIRDKKVAFVGQSGVGKSTLINALLGSDQKTDEIS
                     TKYDRGRHTTNHSLLMRTGNLTIVDTPGVREIFVPHTDLRQIQRAFPEFKRYRCLYDP
                     CLHVEEPECGVKVAVEEEEILFDRYESYLRIVESLEMITPDYLSRNERSEAWIERGAE
                     YDWEES"
     gene            657..1379
                     /locus_tag="PKN79_02720"
     CDS             657..1379
                     /locus_tag="PKN79_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94706.1"
                     /translation="MTGKSLDDLEFAKVQTMISSFRLSEEGGEFLASLPFAMEKEELT
                     LRQGYISRILGSFERDGVPALAHFPSIADVMDRLGEPTPSLDGPGLLDLARYLQSSEE
                     LFDYLLRDEEEALRELIGPGIDPALIALKEAILADLDESGSVRENHPRLQPLYRQLEQ
                     VRQKRASYCSSFIRDNRSSVQSDVEALRDAAGDSDQRRLQAQRQRVYLLRFIKRPDGV
                     HGAVHPCRPEQLRPLGEQPDSG"
     gene            1312..2973
                     /locus_tag="PKN79_02725"
     CDS             1312..2973
                     /locus_tag="PKN79_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNZ94707.1"
                     /translation="MEPFTLVDLNNSVLLANNRILVEIAKILQELALKAKQEEKLLHE
                     LRDTVGTADGYYALSRWAHEQRCTIVRLDSDEVRLLDARHPLLKQKAIPITIILEKGI
                     NGVVLSGPNAGGKTVTVKTFGLFALLNQYCGYLPVREGSALPLFDRVFTDIGDEQSIE
                     KELSTFSGHMSRLSEILSHLTPNSLVILDELGSGTDPLEGSALAQAILSYSLKKSALT
                     LVTSHHGVLKEYAYSKENIINAAMEFDEVSGKPTFRIVPNVIGQSHALVTAQAMGLPE
                     EVLSEARELLGSDRIELSSILMRLEEERKRVEERSKALSKIESRWKEESRKLDLKALT
                     IKQQERQLKDEEIRTLARFTQKARQRLEQLVTDIREGELTGEKTKAVKTYLAELDRRV
                     ETEEAALEKTEEAFVHPTTFEVGMEVLCGPSKREGTIIKKEGRNRYLVAIGSMRMILK
                     ESDLYLPRNRESTVKVSYTANARRAKQEIDVRGFTLEEALEAVEQNIEAALLNNLSTF
                     GIIHGYGDGILSQGIGRLLKNHRSVVDYRFALPEDGGMGKTYVFL"
BASE COUNT          742 a          828 c          849 g          620 t
ORIGIN      
        1 cgccaatcca cccttccgac cccgcttcat cgatcgggtg atcgcttcgg cgatcgatgt
       61 ggaggtgatc atcctcttga acaaatgcga tcttgagatg aaggaggagg atgcgttccg
      121 ttttgagcat tatggcgccc tcggattcca gaccgcggcg gtaagcgccg agaccggaga
      181 aaaccttgat cgacttgttc agatcatcag ggacaagaag gtcgctttcg tcggccaaag
      241 cggggtgggc aagtccacct tgatcaacgc ccttcttgga tccgatcaga agaccgacga
      301 gatctcgaca aaatatgacc gcggcaggca tacgaccaac cactccctgt tgatgcgcac
      361 ggggaacctg acgatcgtcg ataccccggg agtccgcgag atcttcgttc cccacaccga
      421 tctccgtcaa atccaacggg cgtttcccga gttcaagcga taccggtgcc tgtacgatcc
      481 ctgccttcat gtggaggagc cggagtgcgg agtgaaagtt gccgttgaag aggaggagat
      541 cctcttcgat cggtatgaat cctacctgag aatcgttgaa tcccttgaga tgatcacccc
      601 cgactatctt tccagaaatg aacggagcga agcgtggatt gaacgaggag cggagtatga
      661 ctgggaagag tcttgacgat ctcgagttcg ccaaagtcca aacgatgatc tcctccttcc
      721 ggctcagcga ggagggcgga gagtttctcg cctcccttcc gtttgctatg gaaaaagagg
      781 agcttacgct caggcagggg tatatctccc gcatcctcgg atcgttcgag cgggatggag
      841 ttcccgcctt agcgcacttt ccctcaatcg ccgatgtgat ggatcgcctc ggtgaaccga
      901 ctccctcgct ggatggcccc ggtcttctgg atcttgcccg ctatcttcaa tcctccgaag
      961 agctgtttga ttatcttctt agagatgaag aagaagctct gagggaactg atcggtccgg
     1021 ggatcgatcc cgccctcatc gctctcaaag aggcgatcct tgccgatttg gatgaaagcg
     1081 gttcggttcg ggagaaccat ccccgcctgc agcccttata ccggcagctc gagcaggtcc
     1141 gacagaagcg cgcctcctac tgttcctcct tcatccgcga taaccgctcc tctgtgcaaa
     1201 gcgacgtgga agcgctgagg gacgcggctg gtgattccga tcaaagacgg ctacaagcac
     1261 aacgtcaaag ggtatatctc ctccgtttca tcaagcggcc agacggtgtt catggagccg
     1321 ttcacccttg tcgacctgaa caactccgtc ctcttggcga acaaccggat tctggttgaa
     1381 atcgcgaaaa tccttcaaga acttgctctc aaggccaaac aagaagaaaa actgctgcac
     1441 gaactcagag ataccgtcgg taccgctgac ggatactacg cgctctcccg ctgggcccat
     1501 gaacagagat gcacgatcgt ccggcttgac agcgatgaag tccggcttct cgacgcccgt
     1561 catcctcttt tgaaacaaaa agcgattccg atcacgatca tcctggagaa ggggatcaac
     1621 ggagtcgtcc tctcgggacc gaacgccggg gggaagaccg taacggtgaa gaccttcgga
     1681 ctctttgccc tgctgaacca atactgcggc tatcttcccg tccgggaagg aagcgccctt
     1741 ccgctgttcg accgggtgtt taccgatatc ggtgatgagc aatcgattga aaaagagctt
     1801 tcgacgttca gcggccacat gagccgtctt tccgagattc tcagtcacct caccccgaac
     1861 tcgctggtca tcctcgatga gctcggttcg ggaaccgatc ccctggaggg atccgccctc
     1921 gctcaggcga ttctctccta ttcactgaag aaaagcgccc tcaccctggt aaccagccac
     1981 cacggcgtgc tgaaggaata cgcctattca aaagagaaca tcatcaacgc cgcgatggag
     2041 ttcgatgaag tcagcggaaa gccgaccttc aggatcgttc ccaatgtgat cggccaatcc
     2101 catgcgttgg ttacggctca agcgatgggg ttgccggaag aagttctttc agaagcgcgg
     2161 gagctgctcg gcagcgaccg aatcgaactt tcctcgattc tcatgcgctt ggaggaagag
     2221 cgcaagcgcg tcgaggagcg gagcaaagca ttgtcgaaga tcgaaagcag gtggaaggag
     2281 gagtcccgaa agctcgacct gaaagcattg acgatcaagc aacaggagcg ccagctgaaa
     2341 gacgaggaaa tccggaccct cgcccggttc actcaaaaag ctcgacagag attggaacag
     2401 ctggttaccg atatccgtga aggagaattg accggtgaaa aaacaaaagc ggtaaagacc
     2461 tatctcgccg agctggaccg acgggttgaa acggaagaag cggcgctgga aaaaacggag
     2521 gaggcgtttg tccatccgac gaccttcgag gtcggcatgg aggtcctctg cggaccttca
     2581 aagcgggaag gcacgatcat caagaaggag gggaggaatc gctatcttgt cgcaatcggt
     2641 tcgatgcgga tgatcctgaa ggagagcgac ctgtaccttc cgagaaacag ggaatcgacg
     2701 gtaaaagttt cctacaccgc caatgcgaga cgggcaaaac aggagatcga tgtgcgcggc
     2761 ttcacgctgg aagaagcctt ggaagcggta gagcagaata tcgaagcggc cctgttgaac
     2821 aacctttcga cgttcggaat catccacggc tacggcgacg ggattctcag tcaagggatc
     2881 ggcaggcttc ttaaaaacca ccgttccgtc gtcgattacc gatttgctct tcccgaggat
     2941 ggagggatgg gaaaaaccta tgtattcctg tagaattgag agataaggag tcgatatgag
     3001 tgactatacc catggaagcg gaattcagta tcacctcgg
//
LOCUS       DAOPUZ010000116         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_33448_length_3029_cov_3.175857, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000116 DAOPUZ010000000
VERSION     DAOPUZ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33448_length_3029_cov_3.175857"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            552..1304
                     /locus_tag="PKN79_02730"
     CDS             552..1304
                     /locus_tag="PKN79_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ94708.1"
                     /translation="MSKIYAPSRQHHIKAVNDLSFTIDKGEFVTLVGPSGCGKSTTLR
                     MIAGFLRPTSGDILIEGKSITALGPEMRNIPMVFQNYALFPHMTVFENIAYGLEAKNL
                     PFDAVAHDVAMICQMLNLVGTETRHPNELSDGQQQRVALARALVLKPKIMLFDEPLSN
                     LDPRLRIQTRFEIKRMQQLLGITVLYVTHDQAEALSIPDRILVMNRGTIVQEGSPEPC
                     TIAPAPRSWRTSSAMRTSLTDLFFPHPQRRSS"
     gene            1316..1645
                     /locus_tag="PKN79_02735"
     CDS             1316..1645
                     /locus_tag="PKN79_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNZ94709.1"
                     /translation="MEFTVSRDRCSIFVEQDDSLLVAINPISVGMSPFEGECGFNQCV
                     GIVRSSLFVGQSFEYRVSFGDETIRVLHPNLLGGVREFEEGTPVILTFYPESFNLYGR
                     EVDHHER"
     gene            1710..2618
                     /locus_tag="PKN79_02740"
     CDS             1710..2618
                     /locus_tag="PKN79_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ94710.1"
                     /translation="MSKILVVGCMVLYLALRPTDRWVVRLVRTLLLWGVYALVSSSRA
                     LDVLTVYAGASIVAAWTDRRIRWNRKHRTQPGYEQLLSTLAHEIRTPLTIMQTTQSVL
                     MEQSAGKLNEQQRKFMESIYINTQRLITFSEQMLTLIKLGKDWEPDMTKSVDLKLLVR
                     QVIETLQPQLSIRKQEVSLQFPALVSHPRADEAWIRQVLINLIHNASKNTKEGGLIIV
                     SVTQDDRQIVTTVTDNGQGIELAGREALFQEFFQERKDSQQDGFGLGLSIVRSIIAKH
                     QGKVYITSTPNQGTMVSFSLRVGAPW"
     gene            2615..>3029
                     /locus_tag="PKN79_02745"
     CDS             2615..>3029
                     /locus_tag="PKN79_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ94711.1"
                     /translation="MKNRILVVDDEQAIVDLLHFILTNAGYEVASTGRGDEVAPLARE
                     FHPDLVILDIGLPGLSGLEVVPLLVELDTPVLVLSSHDRDDQIIEGLEVGAEDYVSKP
                     FNHKELLLRIEKIIRRTKVEPNSSTPRFLVNRWGFG"
BASE COUNT          659 a          797 c          859 g          714 t
ORIGIN      
        1 catactcctc ttcgaacgcc tcgatgacgg agtcgggggt gtagtacgac cagttgtaca
       61 catagagctt ctgatttccg ttttcgtcgg tgctttcctt ggcgcacccg gtaagcagaa
      121 gaacggtgac cgcgagagcg atcatgagtt tggtggcagg tttgaacatg gcaaacctcc
      181 tgtctgggtt atgctgcccg ctgaggagaa catacgccat accctcgggc ggtggtgggt
      241 tttcagatta gtggacgaag caatatctct tcatattctt ggcgtttttt taatctgacc
      301 tatcattatg ctttgaagga tttgagtcaa ttgtttttcg ggtcaatcgg aaaaagtttt
      361 tacgatttac ccttgtgttc accgaggggc ctgcccggaa tcaggcaccc ccttttcaac
      421 aggaacgtgt tgagatgaac gtatttccgt ggtagaacga tagatgcaac ctgtccggtt
      481 catgagaatg gaaaatgagt gaattcaaga aagatgaaaa acgtgccgct ccttttttgg
      541 agatgaaaaa ggtgagtaaa atctatgctc cctctcgtca acatcatatc aaagcggtca
      601 atgatctctc gtttaccatc gacaagggtg agttcgtaac gcttgtcggc ccctccggct
      661 gcggaaagtc gaccacgctt cggatgatcg ccggctttct caggccgacg agcggcgaca
      721 tcctcatcga aggcaaatcg atcacggcgt tgggaccgga gatgagaaac attcccatgg
      781 ttttccagaa ctacgccctc tttccccata tgaccgtgtt tgaaaacatc gcctatgggc
      841 tggaagcgaa aaacctgccg ttcgatgcgg tcgcgcatga tgtggcgatg atctgtcaaa
      901 tgcttaatct ggtcgggacc gagacccgcc atcccaatga gctgtccgac ggacagcagc
      961 agcgggtcgc gcttgcccgg gcgttggtcc ttaaacccaa aataatgctg ttcgacgaac
     1021 cgctttcaaa ccttgatccg cgcctgcgga ttcagacccg cttcgagatc aagcggatgc
     1081 agcagcttct ggggatcacg gttctctatg tcacgcacga ccaggcggaa gcgctgagca
     1141 ttcccgatcg gatcctggtg atgaaccggg gaacgatcgt gcaggaggga agccccgagc
     1201 cctgtacgat cgccccggca ccccgttcgt ggcggacttc atcagcaatg cgaacttctt
     1261 tgacggattt gttctttccg catccgcaga gacgatccag ctgagcattc aagagatgga
     1321 attcaccgta tcccgggacc gatgttccat cttcgtcgag caagacgatt cccttctggt
     1381 ggcgatcaac cccatttccg tcggcatgtc accgtttgaa ggcgagtgcg ggttcaatca
     1441 gtgcgtaggc atcgttcgct cttccctctt cgtcggccag tcattcgaat atcgggtaag
     1501 cttcggtgat gagacgatcc gggtactgca tccgaacctg ctcggaggag ttcgggagtt
     1561 tgaagagggc acgccggtga ttctcacctt ttaccccgag tccttcaatc tctatggccg
     1621 cgaggtggat catcatgaac gctaggctcg tcggcaggca gatccttgag cttgttctcg
     1681 catccttggg gacctgtctt cttctctctg tgagcaagat tcttgtcgtc gggtgtatgg
     1741 ttctgtatct cgctcttcgc ccgacggatc gatgggtggt ccgcctcgtt cggacacttc
     1801 tgttgtgggg ggtctatgcc ctcgtttcat cctcccgggc tttggatgtg ctcacggtat
     1861 atgcaggtgc atccatcgtc gcggcatgga cggatcggcg catccgttgg aatcgcaagc
     1921 accgaacgca acccgggtat gagcagcttc tatccacgct cgcccatgag atcaggactc
     1981 cgcttacgat catgcagacg acgcaaagcg ttctgatgga acagagcgcg ggaaagctca
     2041 acgagcagca gcggaagttc atggaatcca tctacatcaa cacgcagcgc ctgatcacct
     2101 tcagcgagca gatgctgacg ctgatcaagc tcggaaagga ttgggaaccc gacatgacga
     2161 aatcggtcga tctcaagctc cttgttcggc aggtcatcga aacactgcag ccccagcttt
     2221 cgatccgaaa acaggaggtc tcgctgcagt ttcccgccct ggtgtcccac ccgagggcgg
     2281 atgaggcatg gatccgccag gttctgatca atctcatcca taacgcctca aaaaacacga
     2341 aggagggagg actgatcatc gtctccgtca ctcaggatga ccggcagatc gtgacgacgg
     2401 tcaccgacaa cgggcaggga atcgagctgg cggggaggga ggcgttgttt caagagtttt
     2461 ttcaggaaag gaaggactcg cagcaggacg gattcggtct tggactctca atcgtccgtt
     2521 ccatcatcgc caagcatcag ggaaaggtgt atatcacaag cacacccaat caggggacga
     2581 tggtctcctt cagtctacgg gtaggtgcgc catggtgaaa aaccggatcc tcgtcgtgga
     2641 tgacgagcag gcgatcgtgg atcttttgca cttcattttg acgaacgcgg gatatgaagt
     2701 cgcctctacc gggcgagggg atgaggtggc gcccctggcc cgggagtttc atcccgatct
     2761 ggtcattttg gatatcggtc ttccgggctt gagcggattg gaagtggttc cactgcttgt
     2821 cgagctggat actccggttc tcgtgctcag cagccatgat cgggatgacc agatcatcga
     2881 ggggttggag gtcggggcgg aggattatgt ctcaaagccg ttcaaccaca aggagctgct
     2941 gcttcgaatc gaaaagatca tccgtcggac gaaggtcgag ccgaactcct cgacaccgcg
     3001 gtttctggtg aacaggtggg ggttcggat
//
LOCUS       DAOPUZ010000117         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_33627_length_3019_cov_3.428812, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000117 DAOPUZ010000000
VERSION     DAOPUZ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33627_length_3019_cov_3.428812"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="PKN79_02750"
     CDS             complement(<1..1066)
                     /locus_tag="PKN79_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94712.1"
                     /translation="MLEDDHYSEYALLWKILHVSRVYDVENQIEMTVWEEWRNETIRT
                     GERVRDGMRIGVTKALMLLGSGFLANPENGALRQKLNKGELTAEDLYHQLLRLVYRFL
                     FLFALEERRAENGLRLVFIPDPSLVKERNLYEKGYSLDRLRDVMLRRSSYNYYTDLWA
                     GQKIVFTALAKGEKRLALPALGGLFSSGMCDDLDGATLDNATFLKAMESLRWARNTSG
                     QYSYIDYKNMGTEELGSVYESLLELVPTVDMPARIFRFIGVEDDESTAGNVRKTTGSY
                     YTPSFLVDQLIKTALVPVIEKTVEENGRNTVEALLSLSVIDPSCGSGHFILAAARKIA
                     EYVALARDDDGVVTPSEYRRA"
     assembly_gap    1173..1182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1183..1670)
                     /locus_tag="PKN79_02755"
     CDS             complement(<1183..1670)
                     /locus_tag="PKN79_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94713.1"
                     /translation="MARRSSHGISWQTIRLEGSLLLPDLLEQIAKNEAIHQKDSEYQI
                     PPGLRMIEEIGRSYQIARALYADFKNRRQGSNTDAFQLTDTFFVKFMTNCLGWEKAQR
                     VRSKRIGEYGYPVQKIAFEKIPITVAPYDMGLDTSDSRFGISGSKVRNRSLFQMSQEY
                     LNA"
     gene            complement(1681..>3019)
                     /locus_tag="PKN79_02760"
     CDS             complement(1681..>3019)
                     /locus_tag="PKN79_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNZ94714.1"
                     /translation="KTRFPNYTVEIVTGELSAEERQDRIAEMGKLEKVILVATDCLSE
                     GINLQDYFNAVVHYDLAWNPTRHEQREGRVDRFGQKFPEVRCTMMYCEDNPIDGFIIN
                     VILRKATTIKQELGVLVPIPENSEAVGNALVQAALLKKSFMKEYGQLSFDFGEIQQAT
                     DAFEEPWRDAREKAQRNRTIFAQRSLHPEDVIPEWEEEQRLLGSGDTIREMMQTLLQR
                     LSNPLKIISEKEFELDPSHLPDELKERFEDASYTKPTRLSLKNPAPIGAEFLHRSHPI
                     VEILSDYVVEHTLDYRNENPIGGRCAVIETSEVDQAYSLFLIRIRHQIATRLNDRSRF
                     LMAEELIVVGSRGMVHPEWIAEKDALKLFSCKPSGTLSRGVQERNIEEALKFYRSEED
                     TIKQICTEHAAKLLERNRRVRSAASARGTVTVNPCFPADLMGVYVLLPSVDSL"
BASE COUNT          713 a          887 c          692 g          717 t
ORIGIN      
        1 gtgcccgccg gtactccgaa ggagtcacca ccccgtcatc atcacgggcg agtgcgacat
       61 actcggcgat ctttcgggcg gcggcaagga tgaagtgacc gctgccgcaa gaaggatcga
      121 tcaccgatag ggagagaagc gcctccaccg tattccgacc attctcctcc accgtctttt
      181 caatcaccgg aaccaaggcg gtcttgatca gctgatcaac gaggaagctc ggagtatagt
      241 agcttccggt agtcttcctg acattgcccg ccgtcgattc atcatcctcg acgccgatga
      301 atctgaatat cctggccggc atatccacgg taggaaccag ttccaacaga ctctcgtata
      361 cagagcccaa ttcctcagtt cccatgttct tgtagtcgat gtagctgtat tgccctgacg
      421 tgttccttgc ccatcgaaga ctctccatcg ccttcaggaa cgtggcattg tccaaggtcg
      481 caccgtcgag gtcatcgcac atcccggaag agaacaatcc tcccagagcc ggcagggcca
      541 accgtttctc acctttcgcc aaagcagtaa acacaatctt ctgccccgcc cagagatcgg
      601 tgtagtaatt gtaggagctt cggcgaagca tcacatcccg aagccgatca aggctgtacc
      661 ccttctcata caagttccgc tccttcacca gagaaggatc ggggatgaac accaggcgca
      721 acccgttctc cgcccgccgt tcctccagag caaacaggaa caggaaccga tagacaagtc
      781 ggagcagttg atgatagaga tcttctgccg tcagctcgcc cttgttgagt ttttgccgta
      841 gcgctccatt ctccgggttt gccagaaacc cggaaccaag caacatcaac gctttcgtaa
      901 caccgatccg cattccatcc cggacacgct caccggtgcg tatcgtctcg tttctccact
      961 cctcccagac ggtcatttct atctgattct caacgtcgta gacccgagaa acatgaagaa
     1021 tcttccaaag cagcgcatat tcggagtagt gatcgtcctc aagcatcgaa tcgaggtcga
     1081 actccagata ttgaggacgt gccagagtcg gagaagacct gagcaaccga atggtcgatc
     1141 cattggatat cacaccccag aagctctttc ccnnnnnnnn nngcattgag atattcctga
     1201 gacatctgga acagcgatcg attgcgaacc ttcgacccac tgatcccgaa cctcgaatcg
     1261 gaagtatcaa gccccatatc gtagggggct accgtaatgg gaatcttttc aaacgctatc
     1321 ttctgaaccg gataaccata ctcacctatt cgcttggatc gcactctttg agccttctcc
     1381 caccccagac aattggtcat gaacttcaca aaaaacgtat cggtaagctg gaacgcatcg
     1441 gtatttgaac cttggcgtcg attcttgaag tccgcatata acgcacgggc tatctggtat
     1501 gatcggccga tctcctcgat catccgcaac cccggaggaa tctgatactc tgaatccttc
     1561 tggtgaatag cctcattctt tgcaatttgt tccaaaagat ccggcaacaa cagggaacct
     1621 tccaagcgga tcgtctgcca tgatattccg tgtgaactac gcctagccat tgaaaactcc
     1681 ttacaacgaa tcaacggacg gaagcaggac atacactccc atcaaatcgg cggggaaaca
     1741 gggattcacc gtcaccgtgc ctctggcgct tgcagcggaa cgaactcttc ggtttcgttc
     1801 caacagcttg gctgcatgct cggtgcaaat ctgtttgatc gtgtcctcct ctgaacggta
     1861 gaacttgagc gcttcttcga tattccgttc ctgtacaccg cgcgagaggg ttcctgaagg
     1921 cttgcaggaa aacagcttca gggcatcctt ctcggctatc cattccggat gtaccattcc
     1981 cctgctgccg acgacgatca gctcctctgc catcaggaag cgagatctgt cattaagcct
     2041 ggtcgctatc tgatgccgga tgcggatcag gaacagggaa tacgcctgat caacctcgga
     2101 ggtctcgatg accgcacacc tgccaccaat cggattttca tttctataat ccaacgtatg
     2161 ttccaccaca taatcactga gaatctcaac gatgggatgg cttcggtgaa ggaactcggc
     2221 accgatgggt gccggattct tcaaggacaa tcttgtgggc ttcgtatatg aagcatcctc
     2281 aaaccgctcc ttcaactcat cgggaagatg ggaaggatcc agttcgaatt ccttctcgga
     2341 gatgattttg agcgggttgc tcaaccgctg aagcagtgtc tgcatcattt ccctgatcgt
     2401 atcccctgaa cccagcaatc tctgctcttc ttcccattcc ggaatgacgt cctcaggatg
     2461 cagggaacgt tgggcaaaaa tggtcctatt cctttgcgcc ttttcccggg catccctcca
     2521 aggttcttca aaggcatccg tagcttgctg gatttcgccg aaatcaaagg aaagctgacc
     2581 gtattccttc atgaatgact ttttcagcaa cgcagcctga accaaggcgt tcccaaccgc
     2641 ttcgctgttc tcgggaatcg gcaccaagac tcccagctcc tgcttaatcg tcgtcgcttt
     2701 cctgagaatg acattgatga tgaacccatc aatcggatta tcctcgcagt acatcatggt
     2761 gcaacgcact tccgggaatt tttgtccgaa ccgatccacc cgcccttccc gttgttcgtg
     2821 tcgggtcggg ttccaggcaa gatcataatg aacaaccgca ttgaaataat cctgaaggtt
     2881 gataccttcg gaaaggcagt cggttgccac aagaatcacc ttctcaagct ttcccatctc
     2941 ggcgatccga tcctgtcgct cttccgccga caactcgccg gtgactatct cgaccgtata
     3001 gttcgggaac ctggtcttt
//
LOCUS       DAOPUZ010000118         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_33640_length_3018_cov_2.486331, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000118 DAOPUZ010000000
VERSION     DAOPUZ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33640_length_3018_cov_2.486331"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PKN79_02765"
     CDS             complement(<1..801)
                     /locus_tag="PKN79_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94715.1"
                     /translation="MLLIVSIVSGSLLFAAGKKEAAPNSFLVRVINVLSPEGTPAFSV
                     KDDDGSEHLLYTGIFTDTNFPIEDIAVGDYLEVVVDNSIIHSMRYVSPIIEENRIPLP
                     SPMDEMSLIDRFSYAYGYQLMQMFAQQGLFLDPNYWARGSVDGIHDMTNPDSIGFFEF
                     TDLFGIIDQFQKEIWEVGEAPTSYPDALSLEEVLNLTADDDLFKKFSYTYGYLLVSDL
                     LKLALDANAKLYAQGGLDFAGSSDPLIDEFGMQMTFYEYQMILEEQYQA"
     gene            959..1966
                     /locus_tag="PKN79_02770"
     CDS             959..1966
                     /locus_tag="PKN79_02770"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNZ94716.1"
                     /translation="MVHLPFLLTEDGICRLQEGHLFILDRRRYPFERVEVSCRNAEEV
                     ATALKAMVTQGGGPLEVALKTMIMEARNHERLPLLNQVRHLEEAARLLADARPTNTTM
                     ARSLDEVLSEVEQRKDEPPVKVVEETVRTILDRFAGIYHRIGKLGSTLIDDGDQILTT
                     CFAEHTFLLSLLYAKQAGKNIRVLVSETRPYLQGSRLTAPSLEEAGIPLRVITDGMGP
                     TMIASGQVQLYMTAADLVCRDGTVVNKIGTLANALACSRYGIPYHVLAISPDPDKKTR
                     ADITIEMRDPKEVTECMGTRTTLDTIEGVYPAFDIVDPDLVTRIITPEGILKPSEIKE
                     H"
     gene            1969..2667
                     /locus_tag="PKN79_02775"
     CDS             1969..2667
                     /locus_tag="PKN79_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HNZ94717.1"
                     /translation="MKAIIGGTGFDLLEGLSLKKRLVETPYGGVDLFLGQGEDEGLVF
                     LPRHGSSHSVPPHRINYRAHALALKQLEVIEAIAIYAVGSIDDTLKPGEVGIVSDFVD
                     HTGRSQEHTLFDRPVTHIEVDKPFDEELKKRMLALKPTLLDAGVYVTTNGPRLETKAE
                     IAFFKRIGFSIVGMTLASEVTVLLEAGIRTLALAYSINWAAGVVEEFSFLPDSQLKAL
                     KTEVTQLCRSCLLR"
     gene            complement(2637..>3018)
                     /locus_tag="PKN79_02780"
     CDS             complement(2637..>3018)
                     /locus_tag="PKN79_02780"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNZ94718.1"
                     /translation="GFDDLFWAPHSRHTTIDEEAIGKEEDLIVLAESEEAGTYLLVGS
                     GGRRVFVTGHGEYDALTLDSEYRRDTQAGLPVEEPKNYYEGGSVTVRWRAHANLLVSN
                     WLNYHVYQTTPYDLAHLSRQDLHS"
BASE COUNT          711 a          819 c          813 g          675 t
ORIGIN      
        1 cgcctgatac tgctcctcca agatcatctg atactcatag aaggtcattt gcattccaaa
       61 ctcatcgatc agcggatcac tggagccggc gaagtccaat cccccttggg catacaattt
      121 ggcgttcgca tcaagagcca gtttgagcaa gtcggacacc agaaggtagc cgtacgtata
      181 actgaacttc ttaaacagat cgtcatcggc ggtaaggttc aacacctcct ccaaacttag
      241 ggcatcaggg taggaggtag gcgcttctcc gacttcccaa atctcctttt ggaactggtc
      301 gatgatgcca aagagatcgg tgaactcgaa gaatccgatc gaatccggat tcgtcatatc
      361 atgaattccg tcgacacttc cccgagccca atagttcgga tcaaggaaga gaccctgttg
      421 agcgaacatc tgcatcagct ggtagccgta cgcatagctg aagcgatcta tcaagctcat
      481 ctcgtccatg gggcttggaa gaggaattcg gttctcttca atgatcgggc tcacataccg
      541 catggaatgg atgatcgagt tgtcgactac gacttcaagg taatcaccga cagcaatatc
      601 ctcgatcgga aagttggtgt cggtgaagat tccggtgtac agcaggtgtt cggacccgtc
      661 atcatccttc acggaaaagg ccggcgttcc ttccggagac aagacattga tcacccggac
      721 aaggaacgag ttcggggcgg cttctttttt gccggcggca aataataaag atccggaaac
      781 aatgctgaca atcagcagca taagtattat ggttctcaat tgttttctat acatacctga
      841 tcctcatgaa ttcttatggc ctcggccacg gttcctttta ctgtacgaac cttatgcctc
      901 ctcgtcaatg taccctcaca caattgccac aaaacttcac ctatgctacg ctttttttat
      961 ggttcacctg ccgtttcttc tgaccgaaga tggaatttgc cgcctacagg aagggcatct
     1021 ctttattctt gatcgaagac ggtatccgtt tgaacgggtc gaagtttcct gccgaaatgc
     1081 cgaagaggtc gcaaccgccc tcaaagcgat ggtcacccaa ggcggcggac ccttggaagt
     1141 cgccttgaaa acgatgatca tggaagctcg caaccatgaa cggcttccgc tcttgaacca
     1201 agtccggcat cttgaagaag ctgcccgtct gcttgccgat gcccgaccga ccaatacgac
     1261 gatggcccgc tccctggatg aggtgctttc agaagtggag cagagaaagg atgaacctcc
     1321 ggtgaaggtg gttgaagaga ccgtccgcac gatcctcgat cgttttgcgg gaatatacca
     1381 ccggatcggg aagctgggat cgaccctgat tgatgacggc gatcagatcc tcaccacctg
     1441 tttcgccgaa cacaccttcc tgctttccct gctctatgcc aagcaggcgg gaaaaaacat
     1501 ccgagtgctg gtcagtgaaa caagaccgta tctgcaaggc agccggctta ccgctccctc
     1561 gttggaagag gcgggaatcc cgcttagggt catcaccgac ggcatggggc cgacgatgat
     1621 cgcctccgga caggttcaac tctacatgac tgcggcggat ctggtgtgtc gggacggaac
     1681 ggtggttaat aaaatcggta cgttggcgaa tgcgctggcg tgttcccgct acggaatccc
     1741 ataccatgtt ctggcgatca gccccgatcc ggacaagaag acgcgggcgg atattacgat
     1801 agaaatgcgc gatccgaagg aagtaacgga gtgcatgggc acaagaacca cgcttgatac
     1861 gatcgaaggg gtgtatccgg cgtttgatat cgtagacccg gatttggtca ctcggatcat
     1921 caccccggaa ggaattctca aaccaagtga aataaaggag cattgaagat gaaagcgatt
     1981 atcggcggaa ccggttttga cctgcttgaa ggattgtctc tgaaaaagag gcttgtcgag
     2041 acaccctatg gaggagtcga tctttttctc ggccagggag aggatgaagg cttggtcttt
     2101 cttccccgac acggatcatc ccacagcgtt ccaccccaca ggatcaacta tcgcgcccac
     2161 gccctcgcct tgaagcagct tgaggtgatt gaagcgatcg cgatttatgc ggtcgggtcg
     2221 atcgatgaca cgctcaagcc gggagaggtc ggcatcgtca gcgatttcgt cgaccatacc
     2281 gggcgcagcc aggagcatac cctctttgac aggccggtca cccacatcga agtcgacaag
     2341 ccgtttgatg aggaactgaa aaagcggatg ctcgccttga aacccacgct gttggatgcc
     2401 ggcgtctatg tcacgaccaa cggaccccgc ctggagacga aggcggagat cgccttcttc
     2461 aagcgcatcg gcttctcgat tgtcgggatg accctcgcaa gcgaagtgac ggtgctgttg
     2521 gaagcgggaa tcagaacgct cgctcttgcg tattcgatca actgggctgc cggggtggtc
     2581 gaggagttct cattcctgcc cgacagccaa ttgaaagccc tgaaaaccga ggtcactcag
     2641 ctgtgcagat cctgtctgct cagatgagca agatcatacg gggtcgtctg atagacgtgg
     2701 tagttgagcc agtttgaaac aagcagattg gcgtgggcgc gccaccgtac ggtgacggag
     2761 ccgccttcat agtagttctt cggttcctcc accggaagtc ccgcttgcgt gtcccgccgg
     2821 tactcgctat cgagggtaag cgcgtcatat tcgccgtgcc cggtgacgaa cacgcgccgc
     2881 ccgccgctgc caaccagaag gtatgttccc gcttcttcgc tttcagcgag cacaatgaga
     2941 tcttcctctt ttccgatcgc ctcttcgtcg atcgtcgtgt gccggctgtg cggagcccag
     3001 aagaggtcgt caaagccg
//
LOCUS       DAOPUZ010000119         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_33671_length_3016_cov_3.492739, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000119 DAOPUZ010000000
VERSION     DAOPUZ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33671_length_3016_cov_3.492739"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PKN79_02785"
     CDS             complement(<1..550)
                     /locus_tag="PKN79_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNZ94719.1"
                     /translation="MSVELLSPAGNLEKLRLALEYGADAAYLGFSPFSLRTNAGNFTD
                     ADLDEVSALKERTGKKLYCTVNMLFDQNRLALLEDQLPKMKAWPFDGFIISDMGIVSL
                     LRKHLGEDVELHLSTQASCTNSESARFYRQAGFKRIILGREATLDDIKAIKDKNPDLE
                     VEVFVHGAMCMAYSGRCLLSAHL"
     gene            complement(547..2907)
                     /gene="lon"
                     /locus_tag="PKN79_02790"
     CDS             complement(547..2907)
                     /gene="lon"
                     /locus_tag="PKN79_02790"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNZ94720.1"
                     /translation="MHVTLVDKCGHQAVKHGGFLGLLLVKEDVETETVGAEDLYTVGT
                     VAKIVKKIKLPDGGMSIFISTLKRFEIKQFYPGGKYIIAEVTYLEDIEDDREELRAWT
                     RLLLNEMKSLTKNNQLISEEMRLNMVNIDHPGKLADFIASILNVDRTDQQDILETLVV
                     RSRIEKVLVFIKNEQNIAQVQAKIQARVNKKLEKNQRDYFLREELKSIQQELGLTTNP
                     KAELIERLTKKFKSLPLNEEARETVERELSRLEMMESTSPEYSLTRTYLEIISDLPWK
                     DPKPETFSIDSARRILEKDHYGMKDVKDRILEFLAVRKKKQDTKGSIICLVGPPGVGK
                     TSVGVSIARALKKEYFRFSVGGMNDESEIKGHRRTYIGAMPGKIVQGLRITKSKNPVF
                     LIDEIDKMGISYQGDPASALLEVLDPEQNSAFRDTYLDIPFDVSQVLFIVTANTLETI
                     PRPLLDRMEVIRLSGYTSDEKLAIGRKYLVPKSLEKHGLNKQEIKYTSPILRKIADEY
                     AREAGVRNFEKSLDKINRKVALKLVEQPDLDTPVVIRQEDLVEYLGQPVFVEDEILKA
                     DKIGMALGLAWTSMGGDTLIIEAQNTPGKGEVKLTGQLGDVMQESVSIAHTYIKAHAD
                     RFRIDPLWFDHNNVHLHVPEGATPKDGPSAGITMTVALYSLITNQVMAPNLAMTGELT
                     LKGKVMPIGGLKEKILAAKRNKIKEIIIPQFNKRDLDELEDEVKKGVVFHSVATIEEV
                     LRLAFPEDAKRKLKPFHPSQETSQSQAKEVVSIAEAVARAVKEALS"
BASE COUNT          597 a          836 c          786 g          797 t
ORIGIN      
        1 ccagatgggc gctgagcagg caacggccgc tgtacgccat gcacatcgcc ccatgaacaa
       61 agacctccac ctccaagtcg gggtttttat ccttgatcgc cttgatgtca tcgagggtag
      121 cttcccgccc gagaatgatc cgcttgaatc ccgcttgacg gtagaacctg gcgctttcgc
      181 tgttggtaca ggaagcctgc gtggaaagat gcagttcaac atcttcacca agatgcttgc
      241 ggagcaggga aacgatcccc atgtcgctga tgatgaaccc gtcgaacggc cacgctttca
      301 tctttggcag ctggtcttcc aacaatgcca accgattttg gtcaaatagc atgttcaccg
      361 tgcagtacag ctttttcccc gttctttcct tcagggcgct gacctcgtca aggtcagcgt
      421 cggtgaagtt gccagcgttg gttctcagtg aaaagggaga aaaacccaga tacgccgcat
      481 cggctccata ctccaaggca aggcgcagct tttcgagatt cccggccggg ctcagaagtt
      541 cgacgctcaa gagagggctt ccttcaccgc cctggctaca gcttcggcga ttgagacgac
      601 ctccttggct tgggattgag aagtctcttg ggaaggatgg aacggtttga gctttcgctt
      661 cgcatcctcg gggaaggcaa ggcggaggac ctcttcgatc gtggcgacgc tgtggaacac
      721 cacccctttc ttcacctcat cttccagttc atccagatcc cgcttgttga attgcggaat
      781 gatgatctcc ttgatcttgt tccgcttggc ggccagaatc ttctctttca atcccccgat
      841 cggcatgact ttccctttca gggtgagctc gccggtcatc gccagattcg gagccatcac
      901 ttggttggtg atcaacgagt acagtgctac ggtcatcgtg attcccgccg acggtccatc
      961 cttgggggtc gccccttcgg gtacgtggag gtggacgttg ttgtggtcga accagagggg
     1021 atcgatccgg aatcgatcgg catgggcttt gatgtacgtg tgggcgatgg aaaccgattc
     1081 ctgcatcacg tccccgagct gaccggtgag tttcacttca ccttttccgg gggtgttctg
     1141 cgcttcgatg atcaacgtgt ctccacccat gctcgtccat gccagaccga gcgccattcc
     1201 tatcttgtcc gctttgagga tctcgtcttc gacaaaaacc ggctgcccaa ggtattcgac
     1261 cagatcctcc tgtctgatca cgaccggagt gtccagatcg ggttgctcca ccagtttcag
     1321 ggctactttc cgattgatct tatcaaggga tttctcgaag tttctcaccc ccgcttcccg
     1381 agcgtactca tcggcgattt ttcggaggat cggggatgtg tatttgatct cctgcttgtt
     1441 caggccgtgt ttttccaacg acttgggaac gaggtatttc cggccgatcg cgagcttctc
     1501 atcgctggtg tagccgctga ggcggatcac ctccatccga tccaggaggg ggcggggaat
     1561 cgtttcgagg gtgttcgcgg tgacgatgaa gagcacctgg cttacatcaa agggaatatc
     1621 caagtaggtg tccctgaagg cgctgttctg ctcggggtcg agcacttcca gaagagcgct
     1681 tgcaggatct ccctgatagg agatgcccat cttgtcgatt tcatcgatca gaaacaccgg
     1741 attcttgctc ttggtgatcc tcaggccctg cacgatcttc ccgggcatcg caccgatata
     1801 cgtccgacgg tggcccttga tctcgctctc gtcgttcatc cctccgacgg agaaccggaa
     1861 atactctttc ttcagcgccc gggcgattga aactccgacg ctcgtttttc cgactcccgg
     1921 cggaccgacc agacagatga tcgagccttt ggtgtcctgt tttttctttc gaaccgcaag
     1981 aaactcaagg attcggtctt tgacatcctt catcccgtag tggtcctttt ccaagatcct
     2041 tcgggcgctg tcgattgaaa aggtctccgg tttgggatct ttccaaggca ggtcgctgat
     2101 gatctcgagg taggtgcggg tgagtgaata ctccggggag gtactctcca tcatctcaag
     2161 gcgactcaac tcccgttcca ccgtttcccg ggcttcttcg ttcaacggga gcgatttgaa
     2221 ctttttcgta agccgttcga tcaattcggc tttggggttg gtggtaagac caagctcctg
     2281 ctgaatcgac ttaagctcct cacggagaaa gtaatcgcgc tggttctttt caagcttctt
     2341 gtttacccga gcctggatct tcgcctggac ctgggcgata ttctgttcgt ttttgataaa
     2401 gaccagcact ttttcgatgc ggctccgcac tacgagcgtt tcaagaatat cctgctgatc
     2461 ggtgcggtcg acattgagaa tcgatgcaat gaagtcggca agtttgcccg gatgatcgat
     2521 gttgaccatg ttcaaccgca tctcttcgct gatgagctgg ttgttcttcg tcaggctctt
     2581 catctcgttg agcaacaggc gcgtccatgc tctgagctcc tcgcggtcgt cttcgatatc
     2641 ttcgagatag gtgacttcgg cgatgatgta tttgccgccg ggataaaact gcttgatttc
     2701 aaatcgtttg agggtcgaga tgaatatgct cattccccca tcggggagtt taattttttt
     2761 aacaatcttg gctaccgttc ccaccgtata gagatcttcc gctccgaccg tttccgtttc
     2821 aacgtcctct ttgactaaca gtaaccctaa aaatccacca tgctttaccg cctggtgccc
     2881 acacttatcg acgagggtta cgtgtatatc gccgagtcgc cgctctacga gatcaactgc
     2941 aaagagaaga cctactttgc ttacaccgag ctcgagaaga acggcatcct caaggagatt
     3001 ggcaaccaaa agtgct
//
LOCUS       DAOPUZ010000120         3000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_33922_length_3000_cov_2.972496, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000120 DAOPUZ010000000
VERSION     DAOPUZ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33922_length_3000_cov_2.972496"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..622)
                     /locus_tag="PKN79_02795"
     CDS             complement(179..622)
                     /locus_tag="PKN79_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNZ94721.1"
                     /translation="MNVLEALEKELIKVPLESGEKYAIIRELVDVAAQAKGYDGEALL
                     DAVLARERLGSTGIGNGIAIPHAKSDAVNKVTMVIGVSKIPVDFESIDNQKARIFFLV
                     IAPSKEASAHVELLASIARSCSSGIFRRMLEQAKDADEVIQLFME"
     gene            complement(635..1117)
                     /locus_tag="PKN79_02800"
     CDS             complement(635..1117)
                     /locus_tag="PKN79_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNZ94722.1"
                     /translation="MSVPFKQIVAYIDGSESSMTALMYAIKLAKEHHARATAVYVVNT
                     KALSDLVKSGIFVAVERDEYQRDLYEDADRYLRHARHLAEEKGVEIDTVKLEGSVHAV
                     VKELVKSLGADLLILGGVSDIRSRRDELASETDRMMRTAPCPVLVVREDDDIWDEFER
                     "
     gene            complement(1119..1802)
                     /locus_tag="PKN79_02805"
     CDS             complement(1119..1802)
                     /locus_tag="PKN79_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNZ94723.1"
                     /translation="MATTTLAPTRTNLLKLSEDLKFAKLGHELLDQKRSILVVELLTL
                     VDQAVDFETRVLSSLKEAQNALSDAIMQMGRLRVGNLAGAVNIDYSIRVGSRRVMGVS
                     VPKVETSFIDKSPYFSSEETSILSEVAIDRYRSALTLMGHLAELKVSIMRLAREVKKT
                     IRKVNALEKIVIPRTEETIAWIDSRIEEQDRENVIQLKTIKDRMERIKALNQVLTQTD
                     DDDTITTGV"
     gene            complement(1804..>3000)
                     /locus_tag="PKN79_02810"
     CDS             complement(1804..>3000)
                     /locus_tag="PKN79_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNZ94724.1"
                     /translation="GTDGLSMPGTGMRFLGEPLTLAVSEQMLGRVMNGLGKSRDGSAT
                     PRGYAERDVNGEPINPTAREYPRDFIQTGISVIDGLTTLIQGQKLPIFSGSGLSHNQL
                     AAQIARQAKVRGSGGDFATIFVAMGVKYDVAKYFIDSFEESGVLDNVALFLSLADDPS
                     IERIITPKTALTLAEHLAFDKGKHVLVIMTDMTNYCESLREIGTLRGEIPSRKGYPGY
                     LYSNLAEIYERSGKIIGRSGSITQLPILSMPNDDISHPVPDLTGYITEGQIVFERGMH
                     ARGLYPPINPLPSLSRLMKDGIGEGMTREDHPHLANQLFASYSHVKDVQNLASVIGEE
                     ELTALDQQYLEFGTFFENRFINQGYDEDRSIEETLDLGWEALSKLPSEELQRVTDEEI
                     AQYYRG"
BASE COUNT          623 a          855 c          799 g          723 t
ORIGIN      
        1 acgatgccta cttttttcat atagatcctc gcttgggcaa tggtgatgat ttgagactcg
       61 gtacatataa agcgtatgca tgagaaatga agtttgtata tatgtccttc ttggaaaata
      121 gcgcatctca tgagacttgt ctcatgagat ggtcttattg ggaagggata gcgtgtttct
      181 actccatgaa aagctggatc acctcatcgg catctttcgc ctgttcgagc atccggcgga
      241 agattccgct ggagcaggat cgggcgattg aagcaagcag ttcgacgtga gcggaggctt
      301 ccttcgaggg agctatgacg aggaagaaga ttcgggcttt ctggttgtcg atcgattcga
      361 agtcaacggg aatcttggag acgccgatga ccatcgtaac cttgttgacg gcatcgcttt
      421 tggcatgggg aatggcaatg ccgttaccga ttccggtcga tcccaagcgc tcgcgcgcca
      481 agaccgcatc aagcaacgct tcgccatcgt agccctttgc ctgggctgcg acatcaacca
      541 attctcggat tattgcatat ttctcacccg actcgagggg aactttgatc aattctttct
      601 ctaatgcttc cagtacattc ataggtgtcg ctcctcagcg ctcaaactca tcccaaatat
      661 catcatcttc ccgaacaacg agaaccggac acggggcggt tcgcatcatc cgatccgtct
      721 cgctggccag ttcatcccgg cgggagcgaa tatcactcac accgcccaaa atcaacagat
      781 cggctccaag gctcttgacc agctccttta cgacagcgtg gaccgacccc tccaacttaa
      841 cggtatcaat ctccactccc ttctcttcgg caaggtgccg ggcgtgccga agatagcgat
      901 cggcatcttc gtagagatct ctctggtatt catcacgctc cactgcgaca aagattccgc
      961 tcttgaccaa atcacttagt gccttggtat tgacgacata taccgccgtc gcccgggcgt
     1021 gatgctcttt tgccagcttg attgcataca tcaaagcggt catcgaactc tccgacccat
     1081 caatatacgc aacgatttgt ttaaacggga ctgacatgct atacccctgt ggttatcgta
     1141 tcatcatcat cggtttgcgt caaaacctga ttgagagctt tgattcgctc catccgatcc
     1201 ttaattgttt tcagctgaat cacgttttcc cgatcctgct cttcgatccg gctgtcaatc
     1261 caggcgatcg tttcctcggt tcgaggaatg acgatctttt ccagagcgtt gactttccgg
     1321 attgttttct tcacttcccg ggcaagacgc atgatggaaa ccttcagttc cgccaagtgt
     1381 cccatcaagg tgagggcgct acggtagcga tcgatcgcga cctcgcttaa gatgctcgtc
     1441 tcttcgctgg agaaataggg acttttatcg ataaaggatg tttcaacttt tggcactgaa
     1501 actcccatca cccgccggga tcccaccctg attgaataat cgatattgac cgccccggcc
     1561 aaatttccga ccctgagccg gcccatctgc atgatcgcgt cgctcagggc gttctgcgct
     1621 tctttgaggg aactcagaac ccgggtttca aaatccaccg cctggtcgac gagcgtgaga
     1681 agctcgacaa ccagaatcga ccgcttctgg tcgagcagct catgcccgag cttggcgaac
     1741 ttcaaatctt cgctgagctt gagcaggttg gttcttgtcg gcgccaaggt ggtcgttgcc
     1801 atatcagcct cggtagtact gggcgatctc ttcgtcggtg acgcgctgga gctcttcgct
     1861 tgggagcttg ctcagcgcct cccacccgag atcgagggtc tcttcgatcg aacgatcctc
     1921 atcatacccc tggttgatga agcggttttc aaagaaggtg ccgaactcca gatactgctg
     1981 atcgagggcg gtgagctcct cttcaccgat tacggaggcg aggttctgca catccttgac
     2041 atggctgtag gaagcgaaga gctggttcgc caggtgcgga tggtcctccc gggtcatccc
     2101 ctcgccgatt ccgtccttca tcagccggga cagcgacgga agcgggttga tcggaggata
     2161 gaggccgcgg gcgtgcattc cccgttcaaa gacgatctgg ccttcggtga tgtatccggt
     2221 aaggtccggc accgggtggc tgatatcgtc gttcggcatc gaaagaatcg gcagctgggt
     2281 gatcgatccg ctgcggccga tgatcttccc cgaccgttca tagatctccg ccagatttga
     2341 atagaggtac cccggatacc cttttcggga gggaatctcc ccccgtagcg ttccgatctc
     2401 cctgagcgac tcgcagtagt tggtcatgtc ggtcatgatc acaagaacgt gcttgccctt
     2461 gtcgaacgcc aaatgctcgg caagggtcag ggcggtcttg ggggtgatga tccgttcgat
     2521 cgacgggtca tcggcgagcg agaggaagag cgccacattg tcaaggactc cgctctcttc
     2581 aaaggaatcg atgaagtact tggcgacatc gtacttaacg cccatcgcaa cgaagatcgt
     2641 ggcgaaatca cctccgctgc cccgcacttt cgcctggcgg gcgatctggg cggcaagctg
     2701 attgtgggac aatccgctgc cggagaagat cggcaatttc tggccttgga tcagggtggt
     2761 caatccgtcg atgacggaaa tccccgtttg gatgaaatcc cggggatact cccgggcggt
     2821 cgggttgatc ggctcgccgt tcacatcccg ctcggcgtat cctcgagggg tcgccgaccc
     2881 gtcgcggctc ttgcccaggc cgttcatcac ccgcccgagc atctgctcac tgaccgcaag
     2941 ggtcagcggc tcaccgagga agcgcatccc cgtgcccggc atcgacaggc cgtcggtgcc
//
LOCUS       DAOPUZ010000121         2997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_33968_length_2997_cov_3.152957, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000121 DAOPUZ010000000
VERSION     DAOPUZ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33968_length_2997_cov_3.152957"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKN79_02815"
     CDS             <1..365
                     /locus_tag="PKN79_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNZ94725.1"
                     /translation="AQYVLMESQLVKDAANLLTPRDAPQLGKIFSRIQTGLRDLLEVT
                     VPEIDWLIKRAGELDGCLGAVQVDNGFAGNIMVLLSKEALPSYIDRLEEYEHIFGFKP
                     RWHIYGANDPARVVFPDD"
     gene            372..1160
                     /gene="surE"
                     /locus_tag="PKN79_02820"
     CDS             372..1160
                     /gene="surE"
                     /locus_tag="PKN79_02820"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNZ94726.1"
                     /translation="MKILLTNDDGYTSEGLNTLADVLEKAGHDIWICAPTFERSASSH
                     AMTLREDITITEYQPNRYHCSGKPADCILYASRVGLFGEKPDVVISGINHGYNISTDV
                     LYSGTVAAAREAAINGMKAIAISAAKDEDGRFAFIEGARFVRDNLAFFMDLVDETTII
                     SINVPPRPNGKAKPANLAYLEYHDAIATKKSEEVHVVDPSSVRFGTAVILTLKGGTGP
                     TQRCATRESDFQAVQDGYISITALDVLPSINSAVQKKLASLGDG"
     gene            1160..1510
                     /locus_tag="PKN79_02825"
     CDS             1160..1510
                     /locus_tag="PKN79_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94727.1"
                     /translation="MGAKWESKCSRCGLCCHEKVVSGNTLVIDLDSRCEFYDPKSKQC
                     TVYAERFVESKRCLKMTVWRAMFASYIPETCAYAQWARTGRIRFARKRYLRIIHTKTC
                     PAEDGEDPFYSAFG"
     gene            1559..2713
                     /locus_tag="PKN79_02830"
     CDS             1559..2713
                     /locus_tag="PKN79_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNZ94728.1"
                     /translation="MRFVYLDNNATTPLHEDVIASMNEALALYGNASSMHAFGRDAAF
                     KIEEARSAVADVIGAPSGSIVFTSGASEANNTVFNIFRERIDLGSKRNRIITTEIEHP
                     SIIETVAYLRGRGYRIDLCPVDNTGRVRLDVLERLLAEDVALVSVMFGNNEIGTIQPI
                     KEIVEMAHKVGAYVHSDATQAIGKIEVSMKELGLDYLSLSAHKFYGPKGVGALAVAKD
                     APFTPYIHGGHQESGRRAGTYNTVAIIGMGVAAGIAKRDRKDEEQRLWKMREMLRKGI
                     IERIPNVVINGNQEHCLPGTLDVSFPHAEGESILLYLDLEGIQVSTGSACASGSLEPS
                     YVLLSSGVDIELAHGSIRFSFGRYNTEDDVAYVLEKLPPIIKRLREMSAL"
     gene            2710..>2997
                     /locus_tag="PKN79_02835"
     CDS             2710..>2997
                     /locus_tag="PKN79_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNZ94729.1"
                     /translation="MSNFMAQWVYSPAVKDHFMNPRNTWDEKENFVPDGVGDVGSLAC
                     GDQMRVMIQVENDRIKAIRWLTYGCASAIASTSMLSEMAIGLTLEDAYHLSP"
BASE COUNT          696 a          764 c          866 g          671 t
ORIGIN      
        1 acgcccaata tgtgctgatg gaatcgcagc tggtcaagga tgccgcaaac ctcctgactc
       61 cccgtgatgc tcctcagctc ggaaagattt tcagccggat tcagaccggg ttgcgagatc
      121 ttttggaagt aaccgttccc gagatcgatt ggctgatcaa acgggccggt gaactcgacg
      181 gttgtctggg tgcggtacag gtggataacg gctttgccgg caacattatg gtcctcttga
      241 gcaaggaggc ccttccaagc tatattgacc gtctggagga atatgaacac atcttcggat
      301 tcaagcctcg gtggcatatc tatggggcga atgatccggc gagggttgtg ttcccggacg
      361 attgacgctt gatgaagata ctcttgacaa atgacgatgg atatacaagc gagggactga
      421 ataccctcgc ggatgttctt gaaaaagccg gtcacgatat ctggatctgt gctcccacct
      481 ttgaacgaag cgcgtccagc catgcgatga ctcttcgcga ggatatcacg attaccgaat
      541 atcaaccgaa ccgctaccac tgcagcggaa agcctgccga ctgtattctc tacgcctccc
      601 gcgtcggtct ctttggcgaa aaacccgatg tggtgatcag tggaatcaac catggctaca
      661 acatctccac cgatgttctc tactccggta cggtcgctgc cgcccgtgaa gcggcgatca
      721 acgggatgaa ggcgatcgcg atcagcgcgg cgaaggatga ggacggcagg ttcgcgttca
      781 tcgaaggggc ccggttcgtt cgggacaacc ttgcattttt catggatctc gtcgacgaga
      841 cgacgatcat cagcatcaat gttcctcccc gtccgaacgg aaaggcgaaa ccggcgaatc
      901 ttgcgtacct tgaataccat gatgcgattg cgacgaagaa gagcgaggag gtccatgttg
      961 tagatccttc ctcggttcgc ttcggaaccg cggtgatcct taccctcaaa ggagggaccg
     1021 gtcccacaca acgatgcgcc acccgggaaa gcgacttcca agcggttcag gacggatata
     1081 tcagcataac cgccctcgat gtccttccct caatcaattc cgcagtccag aagaagcttg
     1141 cctccttggg agatggatga tgggtgcgaa gtgggagagc aaatgctcac ggtgcgggct
     1201 gtgctgccat gaaaaggtcg tcagcggaaa tacgctggtc atcgatcttg attcccggtg
     1261 cgagttctac gatccaaaga gcaagcaatg caccgtctat gcggagcgct tcgtcgaaag
     1321 caagcgctgc ctgaagatga cggtatggcg ggcgatgttc gcctcgtata ttcctgaaac
     1381 ctgcgcgtat gcccagtggg cgcgaacggg tcggatccgt ttcgcccgaa agcggtattt
     1441 gcgcattatt cacacaaaaa cctgtcccgc cgaggatggg gaggaccctt tttactcagc
     1501 tttcggttga tggattaatg cccggtcatg ggcgtgaacg ttgagtaaga ggggtatcat
     1561 gagatttgta tatttagaca acaacgccac gactccgctc catgaggatg tgatcgcttc
     1621 gatgaatgaa gcgcttgcgc tctatggcaa cgcatccagc atgcatgcct tcgggcgcga
     1681 cgccgccttc aagatcgagg aagcccgatc ggcagtcgcc gacgtaatcg gcgcaccaag
     1741 cggttcgatc gtcttcacca gcggagcgag cgaggcgaat aatacggtat tcaatatttt
     1801 ccgggaacgg atcgacctcg ggtcgaagcg aaaccggatc atcaccaccg agattgaaca
     1861 tccttcgatc atcgagacgg tagcctatct ccgaggtcga ggatacagga ttgacctctg
     1921 tccggtggac aacaccggtc gagtccgcct tgatgtgctg gagagactcc ttgccgagga
     1981 tgtcgctttg gtttcggtga tgttcggaaa caatgaaatc ggcacaattc aaccgatcaa
     2041 agagattgtt gaaatggccc ataaggttgg agcctatgtg cacagcgatg cgacgcaagc
     2101 gatcggaaag attgaggtgt cgatgaagga gcttggactg gattacctgt ccctgtccgc
     2161 ccacaagttc tacggtccga agggagtcgg agcgcttgcg gtcgcaaagg acgcaccctt
     2221 caccccgtat atccatggcg gacaccagga atcaggccgg agagccggga cgtacaacac
     2281 ggtggcgatt atcggaatgg gcgtcgcagc cgggatcgcc aagcgggatc ggaaggatga
     2341 agagcagcgg ctttggaaga tgcgcgagat gcttcgaaag gggattatcg aacgtattcc
     2401 gaatgtcgtg atcaacggga atcaggagca ctgcctaccg ggaacgttgg atgtgtcttt
     2461 tccccacgcc gagggagaga gtattttact ctacctcgat ttggaaggaa ttcaagtttc
     2521 caccgggagc gcctgtgcct ccggttcact ggagccctcg tatgtattgc tttccagcgg
     2581 ggtcgacatc gagctggccc atggatcgat tcgtttcagc ttcggtcgat acaacaccga
     2641 agatgatgtt gcctatgttt tggagaagct gcctccgatt atcaagcgac tcagggagat
     2701 gtctgcgtta tgagtaactt tatggctcaa tgggtatata gtcccgctgt aaaagatcac
     2761 ttcatgaatc cccgaaacac gtgggatgag aaggagaact ttgttcctga tggagtcggc
     2821 gacgtcggtt cgcttgcctg tggtgatcag atgcgcgtca tgattcaggt ggaaaacgat
     2881 cggatcaaag cgattcgctg gcttacctac gggtgtgcct cggcgattgc atcgacttcg
     2941 atgctgagtg agatggcgat cggtcttaca cttgaggatg cctaccatct ttcaccc
//
LOCUS       DAOPUZ010000122         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_34125_length_2988_cov_4.100580, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000122 DAOPUZ010000000
VERSION     DAOPUZ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34125_length_2988_cov_4.100580"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PKN79_02840"
     CDS             <1..434
                     /locus_tag="PKN79_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNZ94730.1"
                     /translation="PKKQRKVLVKEVGGQTTKIDSAPWPGFPADLLSILTVAATQSRG
                     SILIHEKMFESRMYFIDWLIRMGADIILCDPHRAVVQGPSVLIGSVLSSPDVRAGMAL
                     VIAAAAARGESVIQNIYQIERGYENLSEKLTSLGIDMVRSG"
     gene            complement(813..1934)
                     /locus_tag="PKN79_02845"
     CDS             complement(813..1934)
                     /locus_tag="PKN79_02845"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNZ94731.1"
                     /translation="MSLFVLSLDWRTKERFKLQFTQPYLQLFNLPAALFEALESETPD
                     MIIIDTKEPKITAGQILRHLGESRCSAPIIIFTRDEPVQNFRNPYDLKIRLMHKRTVN
                     WEEVFQEICRIGKTELVKESSPVWSSGLVGESKLMVELRSLLEFYATKDCPVHIYGET
                     GTGKELAAEYLHRRRFPQRHIIAINCSLLNDAVGKSVFFGHAKGAFTDATNELIGLLC
                     QANNSTLFLDEVENLPLIFQAHMLRLLENGEYRRLGDTKLRSSDFRLVTASNEKLLRL
                     VAEGQMRKDFFYRITDAQIHMPPLREHKEDIPLLIRHFYSTLSERRPVTDTSIKLLQS
                     YHWPGNVRQLFSVLRRASLHAKNNRWIEIDEQEFAEEEL"
     gene            complement(2052..2822)
                     /locus_tag="PKN79_02850"
     CDS             complement(2052..2822)
                     /locus_tag="PKN79_02850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNZ94732.1"
                     /translation="MRVYRLPSTYDGEEEITLKGKEAHYLTNVLRLKAGSRLLAQDCN
                     GVRYQGVITASASASCTIRLTVAGDTSASTDALPSYEEPFTPLVLLLCLLKGRKEEQV
                     VRQATELGVGEIVLVSSEYCTVRLDKKNTRARFARLEAQITEALQQSGSRVPTTLLDR
                     VLDLRELTEWWAGRGPLLFLHQSSRSQSKDLSTYAKTFDPNAPIGVLIGPEGGFSEKE
                     CVFLEKAGFLPVFLKSNILRSETAAIYALGSLQELLHR"
BASE COUNT          677 a          750 c          862 g          699 t
ORIGIN      
        1 tccccaaaaa acaacgaaaa gttcttgtca aagaggtggg agggcagacc accaagatcg
       61 acagcgctcc atggccggga tttccggcgg atctgctgag catcctcacc gttgcagcga
      121 cccaatcccg cggttcgatt ctcatccacg agaaaatgtt cgagtcgcgg atgtacttca
      181 tcgactggct gatccggatg ggtgcggata tcatcctctg cgatccccac cgggcggtgg
      241 ttcaggggcc gagcgttctg atcggatcgg tcttgagcag tcccgatgtc cgggcgggga
      301 tggccctcgt catcgcagcg gccgccgccc ggggggagtc ggtcattcaa aatatctacc
      361 agatcgagcg gggatatgag aacctgagtg aaaagctgac ctcgttggga atcgacatgg
      421 ttcgaagcgg ttaaacgttt ttcttttttg tgcacaagtt atccacaagg atactaaaca
      481 ccctataacc cccaagaaac aaataagaaa aacaaaaggg gcgccgtcta aaaatacaga
      541 gataagctat ctgttaagtt tttatattaa ataaaaataa actcttgttt agtggtttgt
      601 tttactctaa aaccgatata agttttatgt ggtttttagt aggttggaca caacttaacg
      661 gaactccctc tacccagcgt atccacaagt tgtccacagg ctgtacagaa gagaaaaaca
      721 tcatgtaatt accttgatag cgagagttag aggttacttt cccgttggat cccgccctct
      781 tgttcatctg ataacatgtg gatagcttgt ccctacagct cttcttcggc aaactcctgt
      841 tcatcgatct cgatccagcg attgtttttt gcatgaagcg atgcccgcct caggactgaa
      901 aaaagctggc ggacatttcc cggccagtgg tagctttgaa gcagcttgat cgatgtatcg
      961 gttaccgggc gtcgttcgga gagggtcgaa taaaaatgcc ggatgagcag ggggatgtct
     1021 tccttgtgct cccttagcgg cggcatatgg atctgggcat cagtgatccg gtagaagaaa
     1081 tctttgcgca tctgtccttc ggcgaccagc cttaagagct tttcattgct ggcggtgacg
     1141 agtctgaaat cgcttgaacg gagcttcgta tcgccgaggc gccgatattc cccgttctcc
     1201 agcaaacgaa gcatatgggc ttggaagatg agcggcagat tctccacctc atccagaaag
     1261 agggtggagt tgtttgcctg gcataggagg ccgatgagtt cgttggtcgc gtcggtgaac
     1321 gctcctttgg catgtccgaa gaacaccgat ttgccgacag cgtcattgag cagcgagcag
     1381 ttgatggcga tgatgtgccg ctggggaaaa cgacggcggt gaagatactc ggcggcgagc
     1441 tcttttcccg tcccggtttc gccgtagatg tgcaccgggc aatctttcgt cgcatagaac
     1501 tcaagcaggc ttctcagctc gaccatcagc ttgctttcac cgacaagacc gctggaccag
     1561 acgggagatg attctttgac gagttccgtc tttccgattc ggcagatctc ttggaacacc
     1621 tcctcccagt tgacggttcg cttgtgcatc aaccggatct tcagatcata ggggttgcgg
     1681 aagttttgaa ccggctcatc ccgggtgaag atgataatcg gggcggagca tcgactttca
     1741 ccgaggtggc ggagaatttg gccggcggta atcttcggct ctttggtgtc gatgatgatc
     1801 atgtcgggag tctcggattc aagggcttca aagagcgcag ccggcaggtt gaagagctga
     1861 agatatggtt gggtaaactg tagcttaaac cgctccttgg ttctccagtc caaggagagt
     1921 acgaatagag acatacgtat actcctatct tgttttaagg gtgtaaagga gaggcaaagg
     1981 agtagaacgc aaccctacca tagtcttacc aaagacattc aacggctatc tactatcatt
     2041 ttgagaggtt tttagcggtg aagaagctct tggaggctgc cgagcgcgta gatcgccgcg
     2101 gtttcgctgc gaagaatgtt gcttttcagg aagacaggga gaaaccccgc cttctccaaa
     2161 aagacgcact ccttctcgct gaaccctccc tcgggaccga tcaggacacc gatcggggcg
     2221 ttcggatcga acgttttggc gtatgtcgag aggtctttgc tttgggagcg ggaggactgg
     2281 tgaaggaaga ggagggggcc tctgccggcc caccactcgg taagctcccg aagatcgagc
     2341 acccggtcaa gcaatgtggt gggaactctc gacccgctct gctgaagcgc ttcggtgatt
     2401 tgagcctcga gtcgggcgaa ccgggcccgg gtattcttct tatccagccg gacggtgcaa
     2461 tattcgctgg agacgaggac gatctcgccg acgccgagct cggtggcctg acgaacgacc
     2521 tgctcctcct tcctgccttt gagcaggcag agcaggagaa ccagcggagt aaagggctcc
     2581 tcgtaggagg gcagggcgtc ggtggaggcc gatgtgtccc cggcgacggt gagcctgatc
     2641 gtgcaggagg cggatgcgga agcggtgatg acgccttggt aacgcacccc gttgcaatct
     2701 tgggcgagaa ggcgtgagcc ggccttgagg cgcagcacat tcgttagata atgcgcttcc
     2761 ttgcccttca gggttatctc ctcctctccg tcataggtag aggggaggcg gtagactctc
     2821 atcgccgggc tcgaatcacg tcaatcgccg cctgcaattg aggatcgaag gcgggatcgg
     2881 ccaacttgcg ttcatcatag ggaatcttcg cctgatactc gttgcggaga agaatcgcca
     2941 ggatctcatc acggattccc cgctcccggt tggcgttcac gaaggcag
//
LOCUS       DAOPUZ010000123         2982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_34231_length_2982_cov_2.874274, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000123 DAOPUZ010000000
VERSION     DAOPUZ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34231_length_2982_cov_2.874274"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKN79_02855"
     CDS             <1..622
                     /locus_tag="PKN79_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNZ94733.1"
                     /translation="IGVVSGLNRPVRTAEGRVVMNAIQTDASINPGNSGGPLLNSRGE
                     VIGINSSIFSTSGSSQGINFAIPIDTAVEVVGELIRTGKVSRGWIDASMLQLTPRLVE
                     YAKLPITQGVLISQLAPGGNAEKAGLKGGTRQVQYGSSIFYLGGDIIVAIDNEKVEDL
                     NGLYLALLPKKSGEKVAVTVNRSGEVKKIDVVLIERTMSHIRALVR"
     gene            624..2693
                     /gene="uvrB"
                     /locus_tag="PKN79_02860"
     CDS             624..2693
                     /gene="uvrB"
                     /locus_tag="PKN79_02860"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNZ94734.1"
                     /translation="MDDRQVVDGSWGGETITNVHGEVSYQERRFQVISEFEPAGDQGQ
                     AIDKLSEGVLEGQRYQTLKGVTGSGKTYTMAKIIERVQRPTLVLSHNKTLAAQLYREF
                     KSFFPDNAVEYFVSTYDYYQPEAYVPGKDLYIEKDADINAEIERLRLSASFSLMERRD
                     VIVVATVSCIFGLVNPVSIRDMVYTFKRGERFNHKEILNQLVRMQYERNDAILKRGSF
                     RVRGDVIEICPSYLENAVRISVDWDEIAEILWFDPISGEKQGSVETFSLYPAKQFVMP
                     PEQVKAAIDRIRLEMEEQVEYFESLGKPLEAERIRTRVEYDLEMLSEIGYCSGIENYS
                     RQLSNRKAGERPAVLLDYFPPDFLTFIDESHVTLPQVGAMYEGDRSRKLNLVTYGFRL
                     PSALDNRPLKAEEFEQVIGQRVFVSATPGKIELEKATNVVEQLIRPTGLLDPKVEVRP
                     TEGQMEDLYGEIRATVAKSQRVLITTLTKKMSEDLTDYLSGLSLKVRYLHSEIETIER
                     VELLRDLRLGVFDVLVGINLLREGLDLPEVSLIAILDADKIGFLRSATSLVQTIGRAA
                     RNADGRVIMYADRHSDAMIEAIRETERRREAQLAYNAEHGITPKTIIKAISDIIEREK
                     ETTQAIQSREIEILKGGYNLLLASGRKKYVKALETEMFEAAKDLEFERAALLRDEIQR
                     IERGEIE"
     gene            complement(2663..>2982)
                     /locus_tag="PKN79_02865"
     CDS             complement(2663..>2982)
                     /locus_tag="PKN79_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94735.1"
                     /translation="EAQRIAAANLDRNPRLFVLAATTADNTSKEPRFGSPIHGYFTKA
                     LLEALDTTSKKVTLDQIYHYIHEHQGFPTEGSNKLLYQHPIVSGGALDLVLRYSISPR
                     SIR"
BASE COUNT          687 a          800 c          924 g          571 t
ORIGIN      
        1 catcggggtg gtcagcggtc tcaaccgccc tgtgcggacc gccgaaggcc gggtggtgat
       61 gaacgcgatt caaacagatg cctcgatcaa cccgggcaac agcggaggac cgcttctgaa
      121 ctcccgggga gaggtgattg gaatcaacag ttcgatcttc tcgacctcgg gaagcagtca
      181 ggggatcaac ttcgccattc cgatcgacac cgccgtcgag gtggtcgggg agttgattcg
      241 gaccggcaag gtcagcaggg ggtggatcga cgcctcgatg ctgcagctta ccccccgtct
      301 cgtggagtat gcgaaactcc cgatcaccca aggagtcctg atcagccagc tcgcccccgg
      361 cggaaacgct gaaaaggcgg ggctgaaagg aggaacccgg caggttcaat acgggtcaag
      421 catcttctat ctcggagggg acatcatcgt tgcgatcgat aacgagaagg tcgaagacct
      481 gaacggtctc tacctcgcac tgcttcccaa gaaaagcggg gagaaggtcg ccgtgacggt
      541 gaacaggagc ggcgaggtga aaaagatcga cgttgtcctc attgagcgaa cgatgagcca
      601 tatcagggcg ttggtgcgat aagatggatg accgacaggt tgtagacggt tcctggggcg
      661 gcgagacgat caccaacgtt cacggcgagg tctcctatca ggagcggagg tttcaggtca
      721 taagcgagtt cgagccagcc ggggaccagg gacaggcgat cgataaactg agtgagggag
      781 ttctggaggg gcagcggtac cagaccctca agggtgtgac gggaagcgga aaaacctaca
      841 cgatggccaa gatcatcgaa cgtgttcaac gcccgacgct ggtattgtcc cacaacaaga
      901 ccctcgctgc ccagctgtat cgggagttca agtcgttctt tcccgacaat gcggtggagt
      961 acttcgtttc gacctacgat tactaccagc cggaagcgta cgttccgggc aaagacctgt
     1021 atattgaaaa ggatgccgat atcaacgccg agatcgaacg cctgaggctt tccgcatcgt
     1081 tttccctgat ggaacggcgg gatgtcatcg tcgttgccac cgtcagctgc atcttcggct
     1141 tggtaaaccc cgtttccatt cgggacatgg tttatacgtt caaaagaggc gagcggttca
     1201 atcacaagga gattcttaat caacttgttc ggatgcagta tgaacgcaac gacgcaatcc
     1261 tcaagcgggg atcgttccgg gtccgcggcg atgtgattga gatctgtccc tcctacttgg
     1321 aaaatgcggt ccgcatctcc gtcgattggg atgaaatcgc cgagattctg tggttcgacc
     1381 cgatcagcgg cgaaaagcag gggagcgtgg agacgttctc cctctatccg gcaaagcagt
     1441 tcgtgatgcc ccccgaacag gtgaaggcgg cgatcgatcg gattcgattg gagatggagg
     1501 agcaggtcga gtattttgaa tcattgggca agcccttgga agcggaacga atccgaaccc
     1561 gcgtcgagta tgatctggag atgctcagcg aaatcggata ctgctcggga atcgagaact
     1621 actcacgcca gctctccaac cgcaaggccg gggagcgccc ggcggtgctg ctcgactact
     1681 tccccccgga cttcctcacc ttcatcgatg aatcgcacgt aaccctcccg caggtcgggg
     1741 cgatgtatga aggcgaccgc tcccgaaagc tgaatctggt gacctatggg ttccgccttc
     1801 cttccgccct cgacaaccgg ccgctgaaag cggaggagtt cgagcaggtg atcggacaga
     1861 gggtcttcgt ctcggcgacc cccgggaaga tcgagctgga aaaagcgacc aacgtggtgg
     1921 agcagttgat ccggcccacg ggtctgcttg atcccaaggt cgaggttcgc ccaaccgagg
     1981 gtcagatgga ggacctctac ggagaaatcc gggcaaccgt ggcgaagagc cagcgggtcc
     2041 tgatcaccac gctgacgaag aagatgagcg aagacctgac cgactacctc agcggtcttt
     2101 ccctgaaggt tcgctatctc cactccgaga tcgagacgat cgaacgggtc gagctcctca
     2161 gggatcttag gctcggcgtc ttcgatgtct tggtcggcat caatctcctc agggaagggt
     2221 tagatcttcc cgaggtttcc ctgatagcga ttctcgatgc cgacaagatc ggattcctgc
     2281 gctcggccac cagcctggtt cagacgatcg ggagggcggc gcgaaacgcc gacggacggg
     2341 tcatcatgta cgccgaccgc cacagcgatg cgatgatcga ggcgatcagg gaaaccgagc
     2401 gccgccgtga agcgcaactt gcctacaacg cggaacacgg gatcaccccg aagacgatca
     2461 tcaaggcgat cagcgacatc atcgagcgcg agaaggaaac gacccaagcg attcaaagcc
     2521 gggagatcga gattctcaag ggagggtaca accttctgct cgcctcggga cgcaagaaat
     2581 atgtcaaagc acttgaaaca gagatgttcg aagcggcaaa ggatctggag ttcgaacggg
     2641 cggcgcttct tcgggatgag attcagcgga ttgagcgggg agagatcgaa tagcggagaa
     2701 cgaggtcgag agccccgccg ctcacgatcg gatgctgata gaggagcttg ttcgaccctt
     2761 cggtgggaaa cccctggtgt tcatggatat agtgatagat ttgatcgagg gtgaccttct
     2821 tacttgttgt gtccagtgcc tccaacagcg ctttggtgaa gtaaccgtga atcggagaac
     2881 cgaatctcgg ctccttgctg gtgttgtccg ccgtggtcgc cgccaggacg aagaggcggg
     2941 gattccgatc aagattcgct gctgcgatac gctgggcctc gg
//
LOCUS       DAOPUZ010000124         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_34310_length_2977_cov_3.380219, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000124 DAOPUZ010000000
VERSION     DAOPUZ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34310_length_2977_cov_3.380219"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PKN79_02870"
     CDS             complement(<1..1053)
                     /locus_tag="PKN79_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HNZ94736.1"
                     /translation="MVELTVPAGSLQSALHAFSEGGDGVYLGLKRFSARKGATNLTIE
                     ELAKLRTVALKENRKIYLTINTLVTDDELNDVIATLKEVDRIGVDGIIVQDLGIARII
                     RNAFPSLPLHASTQLAVHTVEGVRQLQKWGFERIVLARELTLDEIDDIRKACPDVELK
                     VFVHGALCYAFSGLCMASHYLTGRSANLGACAQICRTWWSVEENPSLGPSLSPLGKGP
                     RNAPFFSMSDVNSIEVINRLKAMGIDSLKIEGRMKSPAYAHFSARAYRKALDDEPVDL
                     DPLSITFARTQSTGWLAGYGRSGADFSSRHTPSLSSGNTASHMGVAAGTILDYVDHEI
                     QFALDRPVADRDGVMYL"
     gene            1137..1670
                     /locus_tag="PKN79_02875"
     CDS             1137..1670
                     /locus_tag="PKN79_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNZ94737.1"
                     /translation="MSTTPIVSVTKGTIIYEQGQPCTTMFVLQSGTIGLYLNWATPTQ
                     FQLLEISKVGTMFGEMGLFEKDPRNATAIALTDCTFLEISSESFPRFVQEHPDQTINI
                     ILDLSQRFKMALQEIKASQEIIAESLAALKEASEYKKDTFKDRIKRISDYLLDIPEDV
                     PPELYLSLNSRFHGTMY"
     gene            1791..>2977
                     /locus_tag="PKN79_02880"
     CDS             1791..>2977
                     /locus_tag="PKN79_02880"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNZ94738.1"
                     /translation="MTLNDIKHAKLKAWIEEFAALATPDAIVIANGSQEQYDELIRQQ
                     IADGYCVALNPKKKPGSVAYNSDPSDVARVENRTYIASETKDEAGPTNNWIDPVELKK
                     TMTDLYRGSMKGRTLYVIPFSMGPLGSPIAKIGVEITDSAYVVLNMMIMTRVGVKVLD
                     LLGTDGEFVPCLHSVGKPLAKGESDHGKWPCAPLEDKYISHFPETREIWSYGSGYGGN
                     ALLGKKCLALRIASVLARDEGWLAEHMLILKITNPKGKSIFVTGAFPSACGKTNLAML
                     VPTIPGWKVETIGDDIAWMKFGKDGRLYAINPEAGFFGVAPGTSEKSNYNAMKAAEKN
                     TIFTNCALTEDNDVWWEGIGYDAPGKLIDWTNNEWIQEKGNKAQKPAAHPNARFTAPA
                     KQAP"
BASE COUNT          723 a          821 c          804 g          629 t
ORIGIN      
        1 aaggtacatc accccgtccc ggtcggcgac cggcctgtcc aatgcgaact gaatctcatg
       61 atcgacgtaa tcaaggatcg ttcccgccgc gacacccatg tgggaggcgg tatttccact
      121 gctcaaggag ggggtatggc gggaagagaa atccgctccg cttcggccgt acccggcgag
      181 ccatcccgta ctttgcgttc gagcgaaggt gatggaaagg ggatcgaggt ccaccggttc
      241 gtcatcgaga gcttttcggt aggctcgggc ggaaaagtgg gcgtacgccg gacttttcat
      301 ccgcccctcg atcttgagac tgtcgattcc catcgccttg aggcgattaa tcacctcgat
      361 gctgtttaca tcgctcattg agaagaaggg ggcgtttcgc ggccccttgc cgagcgggga
      421 aagggaaggt ccgagggatg gattctcctc cacgctccac cacgttcgac agatctgggc
      481 gcaggcgccc aggttggccg atcgcccggt aagataatgg ctggccatgc acagtccgct
      541 gaacgcatag cagagcgctc cgtggacaaa gactttcagc tcaacatccg ggcaggcttt
      601 acgaatatcg tcaatttcat ccaacgtgag ctcgcgagcc agcacgatcc gctcgaatcc
      661 ccacttctga agctgcctca ctccctcgac ggtatgaacc gccagctggg tggaagcatg
      721 cagggggagg gaggggaaag cgttcctgat gattcgggct attccaagat cctggacgat
      781 gattccgtcc acgccgattc gatccacctc cttcagggtg gcaatgacgt cgttgagctc
      841 atcatcggtt accagggtgt tgatcgtgag gtagatcttc ctgttctctt tcagagcgac
      901 tgtgcgaagc ttggcaagct cctcaatcgt gagatttgtc gcacctttcc gggcggaaaa
      961 ccttttaaga ccgaggtata ctccatcacc tccttcactg aacgcgtgaa gggcgctttg
     1021 taatgaaccg gctggaacgg tgagttcgac catggattac agcctacggt tctccccggg
     1081 acgtgtcaac gttcctactc tatgatatac ttgtcgtctg agatggagga gatgatatgt
     1141 ctaccacccc aattgtttca gttacgaagg gaacgatcat ctacgagcaa ggacaaccat
     1201 gcacaacgat gttcgttttg caaagtggaa ccatcggctt atacctgaac tgggctacgc
     1261 cgacgcaatt ccaacttctc gagatttcaa aagtcggtac gatgttcggt gaaatggggc
     1321 tctttgaaaa agacccccgc aatgcgacgg cgatcgctct gaccgattgc acgttccttg
     1381 aaatttcaag cgaaagcttt ccccggtttg tccaagagca tcccgaccaa acaatcaaca
     1441 tcattttaga tttgagccag cgcttcaaga tggcgctgca ggagatcaaa gcaagccagg
     1501 agatcatcgc cgaatcgctt gccgctttga aagaagcaag cgagtacaag aaagatacat
     1561 tcaaggatcg aatcaagcga atcagcgatt atctgctgga tatccccgag gatgtgccgc
     1621 cggagctgta tctttccctg aactcccgtt tccacgggac gatgtactga tacatgaaaa
     1681 aaatctcagt gtactgttta ctcttgctaa agagagggaa aaacgatacc gttgaagccg
     1741 tacaacttct ctcgatatga gaaattgacc ctattttaaa ggagaagtcc atgacactca
     1801 atgacattaa acatgcgaaa ctgaaggcat ggatcgaaga gtttgcagct ttggccaccc
     1861 ctgatgcgat cgtaatcgcc aatggaagcc aagagcagta tgacgaactg atccgacagc
     1921 agattgctga tgggtactgc gttgccctta acccgaagaa gaaaccgggt tcagtcgcgt
     1981 acaactccga tccttccgac gttgcacgtg ttgaaaatcg tacctacatc gccagtgaaa
     2041 ccaaggatga agccggcccg acaaacaact ggatcgatcc cgttgaactg aagaagacga
     2101 tgacggatct ctatcgagga agcatgaagg gacgaacgct ctatgtcatt cccttctcaa
     2161 tgggaccgtt gggctccccg atcgccaaga tcggtgtcga gattaccgac agtgcctacg
     2221 tcgtgctgaa catgatgatc atgacccggg tgggggtcaa agtgcttgat ctgctcggaa
     2281 ccgacgggga gttcgttccc tgtctccact ccgtcggaaa gccccttgcc aaaggcgaga
     2341 gcgaccacgg caaatggccc tgtgctccac ttgaagacaa gtatatttcc cacttccccg
     2401 agacccgcga gatctggtcc tacggttccg ggtacggcgg gaacgcgttg cttggcaaga
     2461 agtgcctcgc ccttcgcatc gctagcgtcc tcgcccgtga tgagggatgg ctcgccgagc
     2521 atatgctgat tctcaagatc accaacccca agggcaaatc gatcttcgtt accggcgcat
     2581 tcccctctgc gtgtggaaag accaacctcg cgatgctcgt tccgacgatc cccggctgga
     2641 aggtcgagac gatcggagac gatatcgcct ggatgaagtt cggcaaggac ggtcgcctct
     2701 atgcgatcaa cccggaagcc ggattcttcg gcgtcgcccc cggtaccagc gagaaatcga
     2761 actacaacgc gatgaaggcg gccgagaaga acacgatctt taccaactgt gccttgacgg
     2821 aagacaacga cgtctggtgg gaaggaatcg gatacgacgc ccccggcaag ctgatcgact
     2881 ggacgaacaa cgagtggatc caggagaagg gcaacaaggc tcagaagccc gccgcccatc
     2941 cgaacgcccg cttcactgcc cctgcgaagc aggctcc
//
LOCUS       DAOPUZ010000125         2977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_34312_length_2977_cov_3.180698, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000125 DAOPUZ010000000
VERSION     DAOPUZ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34312_length_2977_cov_3.180698"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKN79_02885"
     CDS             <1..298
                     /locus_tag="PKN79_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNZ94739.1"
                     /translation="SDVQTILLLEGGLSLYDAVTVTFSTMSAAGFTTKNNSIAGFGST
                     YVDIVVTVFMAIGGMNFASTGGSSSARRKASSVTPSSGHIWESWGLRSSFPPHI"
     gene            295..879
                     /locus_tag="PKN79_02890"
     CDS             295..879
                     /locus_tag="PKN79_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNZ94740.1"
                     /translation="MTISKTFPSFWTSLRYSAFQNTSVMTTTGFTSTDYETWPAFSQM
                     ILFFLFFIGGSAGSTAGGIKVVRIVALFKLARYQLKKRIHPKGVFQVRIGETILSPDV
                     ISAIATFVGVYIFTGLIGALILSFSGTDVFTSISASFLCLGNIGIGFAGVGPTDNFGF
                     FPAFYKWVCSFLMLVGRLELFTVYVLFTKTFWKR"
     gene            951..2084
                     /locus_tag="PKN79_02895"
     CDS             951..2084
                     /locus_tag="PKN79_02895"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNZ94741.1"
                     /translation="MKRFLLIPDSFKGTMSSAEICAIVETGIKTHYPDAQVRSIPVAD
                     GGEGSVDAFLQALGGEKKHLTVQGPFGKAMESFYGVLPGNVAVIEMAACAGLPLVGDT
                     LRPDLTTTYGVGELILAAAKAGCKRIIVGLGGSATNDGGCGAAAACGVTFRDKEGNAF
                     IPTGGTLNQVVSIDTSTRNPILDRVTITTMCDIDNPFYGETGAAYIFGPQKGADPEMV
                     KRLDANLRSLAEVIASDIGIDVQAIPGSGAAGGMGGGMAAYFDSELQMGIETVLDAVH
                     FDGLLKETDMVITGEGKIDGQSLRGKVVIGVGRRTKKSGVPVIALVGDIADDADGAYE
                     EGVSAIFSINRLAIPFKEAKLRAKTDLLKTVDNLARFLKTMKW"
     gene            complement(2088..2963)
                     /locus_tag="PKN79_02900"
     CDS             complement(2088..2963)
                     /locus_tag="PKN79_02900"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNZ94742.1"
                     /translation="MYALFNRYSSGDLRSNTGHVDLQRMKTLQGITSCFALFVDGQET
                     PGKWDAVSELHQSFLEQMRGNGETIDHVATGEKTEGCHALLSVEEGGVLEGDLTRLEI
                     LHSWGVRLMTIGWNWENELGFPHYQKGALKPFGHQVVKRMEELKILVDVSHLNDEGIN
                     DIVTIAKRPVVASHSNCRRVCNHSRNLSDELIGKIAETGGVVGLTFCPPFVIRQSTHT
                     SVEALAAHCKHFYKVGGSEILALGTDYDGFTGTCEIRGYQDLHILHSALERVGFSSSV
                     LDGFWYRNARRVLDA"
BASE COUNT          642 a          854 c          817 g          664 t
ORIGIN      
        1 ctcggatgta caaaccatcc ttcttctgga gggcgggctt tccctttatg atgcggttac
       61 ggtcaccttc tcgacgatga gtgccgccgg cttcacgacg aagaataatt cgattgcagg
      121 gtttggaagc acctacgtcg atatcgtggt gacggttttc atggcgatcg gcggaatgaa
      181 cttcgcctct actggcggct cttcgtcggc aaggcgaaaa gcgtcttccg tgacaccgag
      241 ctccgggcat atctgggaat cctggggact tcgatcatcg tttccgccgc atatatgacg
      301 atcagcaaga cgttcccatc tttttggacg agtcttcgct acagcgcatt tcaaaatacc
      361 tcggtcatga ccaccaccgg gtttacctcc accgattatg agacttggcc cgccttcagc
      421 cagatgattc tcttctttct ctttttcatc ggcggctcgg cgggttccac cgccggagga
      481 atcaaggtgg ttcggatcgt cgctctcttc aagctggccc ggtatcagct gaagaagcgg
      541 atccacccca aaggggtgtt ccaagtccgc atcggggaga cgatcctctc ccccgacgta
      601 atcagcgcga tcgccacctt cgtcggggta tacatcttca ccggcctgat cggggccctg
      661 atcctctcct tcagcggaac ggacgtgttc accagcatca gcgcttcatt cctctgtctg
      721 ggaaacattg gaatcggctt tgccggcgtc ggtcccacag acaacttcgg cttctttccc
      781 gctttctaca aatgggtctg ctcctttctg atgctggtgg gccggctcga actcttcacc
      841 gtgtacgttc tcttcaccaa gacgttctgg aagcggtagc gatcgttctt tcttttcccc
      901 tccggaagcg gtacaatatc ttccagcatc atcattgcaa ggagcctcat atgaaacgct
      961 ttcttctgat ccccgattcc ttcaagggaa ccatgagttc cgccgaaatt tgcgcgatcg
     1021 ttgaaacagg catcaaaacc cattatcccg atgcccaggt tcgttcgatt cccgtcgccg
     1081 acggcggtga gggcagtgtc gatgccttcc tgcaagccct cggcggcgaa aagaagcacc
     1141 taaccgtgca aggtccgttc ggaaaagcga tggaaagctt ctacggggtg ctgcccggca
     1201 acgtcgctgt catcgaaatg gctgcgtgtg cgggccttcc ccttgtcggt gataccctga
     1261 gaccggatct gacgacgacg tacggcgtcg gcgagctgat tctcgcagca gcgaaagcag
     1321 gatgcaaacg cattatcgtc ggccttggag gatcggcgac caacgatgga ggatgcggag
     1381 ccgcagcggc ctgcggagtc accttccggg acaaagaggg caacgccttc atccccaccg
     1441 gcggaacgct caaccaagtc gtttcgatcg atacgtcgac gcgaaatccg atcctcgatc
     1501 gggtgacgat taccacgatg tgcgacatcg acaacccatt ttatggtgag acgggtgcgg
     1561 catacatctt cgggcctcag aagggtgcgg accctgaaat ggtgaagcgc ttggatgcga
     1621 acctcagatc gttggccgaa gtgatcgcct ccgatatcgg aatcgatgtg caggcgattc
     1681 ccggcagcgg agcggcaggg ggcatgggcg gtggaatggc cgcatacttt gacagcgaac
     1741 tgcaaatggg aatcgaaacg gttctcgatg ccgtccactt tgacgggctg ctcaaagaaa
     1801 cggatatggt catcaccggg gaagggaaga tcgacggaca atccctgcgc ggcaaagtgg
     1861 tgatcggggt cggccgacga acgaagaaaa gcggtgttcc ggtgattgcg ctggtcggcg
     1921 atatcgccga tgacgcagac ggagcgtatg aagagggagt cagcgcgatc ttttcgatca
     1981 accgcctggc gattcccttc aaggaagcaa agcttcgagc caaaacggac ctgctcaaaa
     2041 cggtcgacaa cctcgcccgc ttcctcaaga caatgaagtg gtagagttca ggcgtccagc
     2101 acccgccggg cgttcctgta ccaaaacccg tccagaacgg acgaagaaaa tccaacccgc
     2161 tcgagagccg aatggaggat atggagatcc tgatatcctc gaatctcaca tgttccggta
     2221 aatccatcgt agtcggttcc gagagccaga atctcggagc cgccaacctt gtagaaatgc
     2281 ttgcagtggg ctgcgagcgc ttcgactgaa gtatgggtcg attgccgtat tacaaaaggc
     2341 ggacagaacg tcagtccgac caccccgccg gtttcagcga tctttccgat cagctcatcg
     2401 ctgaggttcc ggctgtggtt gcacaccctt cgacagttgg agtgactggc gacgaccggc
     2461 cgtttggcaa tcgtgacgat atcgttgatt ccctcatcgt tgaggtggga cacgtcgaca
     2521 aggatcttca gttcctccat ccgcttgacg acctgatgcc cgaagggctt gagagcccct
     2581 ttctgatagt ggggaaaacc aagctcgttt tcccaattcc acccgattgt catcaggcgc
     2641 actccccatg agtggagaat ctccaatcgg gtaagatccc cttcaaggac tcctccttcc
     2701 tcgacgctga gaagggcgtg gcatccttcc gtcttctcac ccgtggcaac atggtcgatc
     2761 gtctccccgt ttccccgcat ctgctcaaga aaggattgat gcagttcact cacggcatcc
     2821 cacttcccgg gagtctcctg cccgtcaaca aaaagggcaa aacagctcgt tattccttgc
     2881 agggttttca ttcgctggag gtcgacatga ccggtatttg atcgaagatc cccgctcgaa
     2941 taccgattga aaagagcata gatcgtgtcg atgtgca
//
LOCUS       DAOPUZ010000126         2953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_34707_length_2953_cov_3.335749, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000126 DAOPUZ010000000
VERSION     DAOPUZ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34707_length_2953_cov_3.335749"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PKN79_02905"
     CDS             <1..466
                     /locus_tag="PKN79_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNZ94743.1"
                     /translation="KEKKHRVEDALSATRAAIEEGVIPGGGAALIQAAKILEKVDLSK
                     LPEDERAGYNIVRRALEEPIRQIAENAGLDGSLIADKCKNEKAGRGFDAENFVWVDMF
                     EKGIIDPVKVTRSALQNAASIAALILTTEAAVTDIPEPPAPMPPGGGMEGMM"
     gene            647..1684
                     /locus_tag="PKN79_02910"
     CDS             647..1684
                     /locus_tag="PKN79_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ94744.1"
                     /translation="MKKLLFTAFLVLMSFTLVFAAGTKEEAAEAAGGSLNAYTTLEEP
                     LAAALFEMFEKETGIHVNFVRLAGGEAVARLEAESANPQVSIWVGGVGLDHITAKTKG
                     LTTPYFSRYAENTPQQYRDPEGSWFGLYVGPLTFVTNLDRAKALGITPPKSWADLLKP
                     EYKGLIRMASPNSSGTAYNVLTTVRYINNGNEDKMMEYLTALDKNIDQYTLSGSAPGK
                     SVAIGEIPIAIGYAHDQVKLKAGGANIEINAPSEGTGYELASMSLVKGGKEAVNAKKL
                     YDWILSSPDAQKKFTEWYVVLVAKGAPKHPDALSIDQIKTVNQDFVWDGDVVNKTRIL
                     DRWTKEIGNKR"
     gene            1716..>2953
                     /locus_tag="PKN79_02915"
     CDS             1716..>2953
                     /locus_tag="PKN79_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ94745.1"
                     /translation="MNKKRFVQFVIAAMVFIAADIWIYVSLMGSFTAYVEEKNYDEVA
                     LFVQTAPEEKGEYAKWLSQVEEIIPGSVAVYLDYRDHEFVPTVGMNAATDALWQQAGK
                     STAFRQALESSYYLETIKLRDQYQVRYDLLEPERGSTIPTDRTKVHIYFVPVIADNGY
                     EALGTVMILVPSTGILGFTRLLRIFAVGAAIAFVAILWAVMLTRDPVTSFMVLGLMVI
                     VLIFVAYPLLEALRLSFMKNGRFSLETWKLGLSPTYIVALWGSLKLGVATATASTIIG
                     FMIAFLVERTAFKRKRLISILATMPVISPPFSLSLSIILLFGNNGLITTQLLKMKTFS
                     IYGLGGLTVVQTISMFPIAFLTISGVLKQIDSTVEDASLDLSATRWQTFRSITLPLAT
                     PGILSAWMLVFTNSLADFANP"
BASE COUNT          660 a          803 c          834 g          656 t
ORIGIN      
        1 gaaagagaag aagcaccgtg tcgaggatgc cttgtcggca acgcgggccg cgattgaaga
       61 gggagtcatc cccggaggcg gggccgcgtt gatccaggcg gccaagattc ttgaaaaagt
      121 cgatctttcc aaactgccgg aagacgagcg ggccggatac aacatcgtcc gacgcgccct
      181 tgaagagccg attcgccaga tcgccgaaaa cgccggcctc gacggctcgc tgatcgccga
      241 caagtgcaag aacgagaagg cgggtcgcgg atttgacgcg gaaaacttcg tatgggtcga
      301 tatgttcgag aagggaatca tcgatccggt gaaggtcacc cgctccgcac tgcagaacgc
      361 cgcctcgatc gccgccctga ttctgaccac ggaagccgcc gtgacggaca ttccggagcc
      421 cccggctccg atgccccccg gtggcggaat ggaaggaatg atgtaggttt tcgccgatag
      481 ataaaagtct gctccgagtc ccctgactca gggcagattt ttttcttggt tctcatgagg
      541 gaatgtcctt gacaacgcgc aagaccattt cctagagtgg tactggacgt cgctttgcag
      601 gaagcggcga atcaccgatt tttttcacac ggaaggagga atttcgatga aaaaattgtt
      661 gtttacagcg tttttggtgc tcatgagctt cacgcttgtg tttgctgccg gaacaaaaga
      721 ggaagcggcc gaggctgccg gcgggtcgct taatgcatac accacattgg aggagccttt
      781 ggcggccgcg ctctttgaga tgtttgagaa agagacgggg atccatgtga acttcgttcg
      841 ccttgccggt ggagaggcgg tagcccgctt ggaagccgaa agcgcgaatc ctcaagtctc
      901 gatttgggtc ggaggtgtgg gtcttgatca catcaccgcc aaaacgaagg gattgacgac
      961 cccatatttc tcgcgctatg ccgaaaatac accccagcaa tatcgcgacc cggaaggatc
     1021 ttggttcgga ctctatgtcg gaccgctcac ctttgtgacc aatctggacc gcgccaaggc
     1081 attgggaatc accccgccga agagctgggc cgatctgttg aagccggagt ataaggggtt
     1141 gatccgaatg gcaagcccga actcctcggg aaccgcctac aacgtactga ccacggtccg
     1201 ctacatcaac aatggcaatg aagacaagat gatggagtac ctgaccgccc tcgacaagaa
     1261 catcgaccag tataccttga gcggatccgc acccggcaag agcgtcgcga tcggtgagat
     1321 tccaatcgcc atcggctatg ctcacgacca ggtcaagctc aaagccggag gagccaatat
     1381 cgagatcaac gctcccagtg aaggaaccgg atatgagctt gcttcgatgt cgctggtgaa
     1441 gggcggcaag gaggcggtca atgcgaagaa actgtatgac tggatccttt cctcccccga
     1501 tgctcagaag aaatttaccg aatggtatgt cgttcttgtc gccaagggcg caccgaagca
     1561 ccccgatgca ctttcgatcg accagatcaa gacggtgaat caggacttcg tctgggacgg
     1621 agacgtcgtg aacaagaccc gcatcctgga tcgttggacc aaagagatcg gcaacaaacg
     1681 ctgatgagct ctgattcgta catagggttt attcagtgaa caaaaaacgc tttgtacagt
     1741 ttgtcattgc ggcgatggtt ttcatcgccg cagacatttg gatctatgtc tcattgatgg
     1801 gttccttcac tgcatatgtg gaagagaaga actacgatga ggttgctttg tttgtccaga
     1861 ccgctcccga agaaaaaggc gagtacgcaa aatggctctc tcaagtcgaa gaaatcatcc
     1921 ccggcagcgt cgctgtctat cttgactacc gggatcatga gttcgtcccg acggtcggga
     1981 tgaacgccgc aaccgatgcg ttgtggcagc aggcaggcaa gagtaccgcg ttccggcagg
     2041 cgttggagtc ctcctactat cttgaaacga taaagcttcg cgatcagtat caagtccggt
     2101 acgatctctt agagccggag cggggatcga cgatccctac cgaccggacg aaggtgcata
     2161 tctacttcgt tcccgtcatc gccgacaacg ggtatgaagc cttgggtacg gtgatgattc
     2221 ttgttccctc gaccggtatt ctcggcttta cccggctctt gaggattttt gcagtcggag
     2281 cggcgatcgc ctttgtcgcg attctctggg cggtgatgct cacccgcgat ccggtaacca
     2341 gcttcatggt tctcggattg atggtaatag tccttatttt tgtcgcctat cccctccttg
     2401 aggcgcttcg tctctccttc atgaagaatg ggcgattcag tctggagacg tggaagctcg
     2461 gtctctcccc gacctacatt gtagccctct ggggatcgtt gaagctggga gtggcaaccg
     2521 ccaccgcttc gacaatcatc ggcttcatga tcgcgttcct cgtggaacga accgccttca
     2581 agcggaagcg gctgatctcc atcctggcga cgatgccggt gatcagtcct ccgttctccc
     2641 tttcgctttc aatcattctc ctcttcggaa acaacgggtt gatcaccacg cagcttttga
     2701 agatgaagac gttctcgatc tacggactcg gcggcttgac ggtcgtccag acgatcagca
     2761 tgttcccgat cgcgtttttg acgatcagcg gggtgctgaa gcagatcgac tcaacggtcg
     2821 aggatgcttc gttggacctc agcgccaccc gttggcagac gttccgttcg atcaccctgc
     2881 cgcttgcgac gccgggaatt ctctcggcat ggatgctggt cttcaccaac agcctggcgg
     2941 acttcgccaa ccc
//
LOCUS       DAOPUZ010000127         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_35025_length_2932_cov_4.007299, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000127 DAOPUZ010000000
VERSION     DAOPUZ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35025_length_2932_cov_4.007299"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PKN79_02920"
     CDS             <1..267
                     /locus_tag="PKN79_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNZ94746.1"
                     /translation="SRLLLRIDAVMKASSYKYVIFNLPEAQLEAVAAVVGGMKSPTVT
                     KLMETGWVSVQIVVAEDTFWEVFEQLKALGAQGILVTAIEKMTE"
     gene            270..1580
                     /gene="hisD"
                     /locus_tag="PKN79_02925"
     CDS             270..1580
                     /gene="hisD"
                     /locus_tag="PKN79_02925"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNZ94747.1"
                     /translation="MPYLERFDNPKGKDLDLFCTRNLLDRSSVEEQVATILAAVNEEG
                     DAALHRYSERFDGVVLSSLAVRSEEFEEARKAVDPDLKEALALAKSNIRAFHEAQRAV
                     GEEIEVMEGVSLKRKVVPIRRVGLYIPGGTAPLFSTVLMLALPAKIAGCKEIVLATPP
                     DREGKIHPAILYAAELCGVDEIYKVGGAQAIGALAFGTETIHRVDKIFGPGNRFVTEA
                     KTQVAQGACAIDMPAGPSEVMVVATNRSNPAFVASDLLSQAEHGPDSQAMVVLIGSDP
                     DRWIEELEEQLERQCSVLKRSGYVKQSLEASRIAVVQEVEEAAFIANAYAAEHLIINT
                     GDRVLDDSLVDLIESAGSIFVGPYSPESAGDYASGTNHTLPTGGWARSMSGVSTDSFV
                     KKITVQELTGDGLARLAPTVMTMAAHEELEAHRRAVEIRLEEER"
     gene            1577..2653
                     /gene="hisC"
                     /locus_tag="PKN79_02930"
     CDS             1577..2653
                     /gene="hisC"
                     /locus_tag="PKN79_02930"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNZ94748.1"
                     /translation="MNELLRKNIVTLKPYSSARTEFTGKADVYLDANEHYRDFVEMKD
                     ANRYPDPKATVLRKKIEEVLGLPFEHTVLGSGSDELIDNLIRSVCTPGKDSILILPPT
                     YGAYRVFADINDVKVEELPLLPSFEIDLPALERFLAKERNERSEGRLKLLFLCSPNNP
                     TGNSYSLETIEAVLKIFDGLVVLDEAYYDFSEGESGVSLLSKYDHLVVLRTLSKAWGL
                     ASARVGIAVGSRELIEVINKIKYPYNLGTPSQALALRALENAEAVKRSLDEIKRERSR
                     LSGALAAFGCVRIVYPSDANFLLLKVDDATRLYTFLTENGTIIRNRTKERGLENCVRI
                     TVGSQAENDILLEGFEAYMRKYYA"
     gene            2646..>2932
                     /locus_tag="PKN79_02935"
     CDS             2646..>2932
                     /locus_tag="PKN79_02935"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606347.1"
                     /note="catalyzes the formation of
                     3-(imidazol-4-yl)-2-oxopropyl phosphate from
                     D-ethythro-1-(imidazol-4-yl)glycerol 3-phosphate and
                     histidinol from histidinol phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase"
                     /protein_id="HNZ94749.1"
                     /translation="MHKKILFVDRDGVIVVEDQVDSFEKIRYIPGVFGGLKRLVDSNQ
                     FYLVLVSNQDGVGTPSFPLEDFEPIHTRIIETLRGEGILFDEEHIDYSLPA"
BASE COUNT          627 a          807 c          912 g          586 t
ORIGIN      
        1 tcccgcctgc tgcttaggat cgatgcggtg atgaaggcct catcatacaa gtatgtgatc
       61 ttcaacctgc ccgaagccca gctggaagcg gtggcggcgg tggtcggggg gatgaagagc
      121 ccgaccgtca ccaagctgat ggagacggga tgggtctcag ttcagatcgt cgtcgccgaa
      181 gacacgttct gggaagtgtt tgaacagctg aaagccctcg gcgcccaggg cattctggtg
      241 accgcgattg aaaagatgac ggagtagcca tgccatactt agagcggttt gacaacccga
      301 aggggaagga tctcgacctc ttctgcactc ggaacctcct tgatcgctcc tcggtggaag
      361 agcaggtggc aacgattctt gccgcggtca acgaggaagg cgatgccgcg ctccatcggt
      421 attcggagcg cttcgacggg gtcgtcctct cttcgcttgc cgtgcgttcg gaagagttcg
      481 aggaagcgag aaaggcggtt gatccggacc tcaaggaagc gctcgccttg gcgaagagca
      541 atatccgggc gtttcatgag gcccaacggg cagtgggtga agagatcgag gtgatggagg
      601 gggtgtccct gaagcgaaag gtcgtcccga tccgccgggt ggggttgtac atccccggcg
      661 gcaccgctcc gctcttttcc accgtcctga tgctggccct cccggcgaag atcgccggct
      721 gtaaggagat cgtcctcgcc actccgccgg acagggaagg gaagatccat ccggcgattc
      781 tgtatgccgc cgagctctgc ggcgtggatg agatctacaa ggttggcgga gctcaggcga
      841 tcggggcgct cgcctttgga acggagacga tacatcgggt cgacaagatc ttcgggccgg
      901 ggaaccgctt tgtgacggaa gcgaaaaccc aagtcgctca aggtgcatgc gcgatcgaca
      961 tgcccgccgg cccgagcgag gtgatggtgg tcgccaccaa ccgctccaac ccggccttcg
     1021 tcgcgtccga ccttctcagt caggcggagc acggccccga cagccaggcg atggtggtgc
     1081 tcatcggttc tgatcccgat cggtggattg aagagctgga ggagcaactt gagaggcaat
     1141 gttcggtttt gaagcggagc ggatatgtga agcaatcgtt ggaggcgagc agaatagcgg
     1201 tcgtccaaga ggtcgaagaa gcggccttca tcgccaacgc ctacgcggcc gagcacctca
     1261 tcatcaacac cggagatcgt gtactggatg attctctggt ggatcttatt gaaagcgccg
     1321 gctcgatctt cgtcggaccc tacagccccg agtccgccgg cgattacgcc agcgggacga
     1381 accataccct gccgaccggc ggatgggctc gttcgatgag cggagtctcg accgattcct
     1441 tcgtgaagaa gatcacggtc caggagctga ccggggatgg ccttgcccgg ttggctccga
     1501 ccgtcatgac gatggccgcc catgaagagc ttgaagccca caggagagcc gttgaaatac
     1561 gattggagga agagcgatga acgaactgct cagaaaaaat atcgtcaccc tgaaacccta
     1621 ctcgagcgcc cgaacggagt tcaccggaaa agcggacgtg tatctggacg ccaatgagca
     1681 ctaccgggat ttcgtcgaga tgaaggatgc gaaccgttac cccgatccga aggcgacggt
     1741 tcttcgaaag aagatcgagg aggtcctcgg tcttcccttc gaacacacgg ttctgggcag
     1801 cggcagcgat gaattgatcg acaacctcat ccgctccgtc tgcacgccgg ggaaggattc
     1861 gatcctgatc ctccctccga cctacggggc gtacagggtc ttcgccgaca tcaacgacgt
     1921 gaaggtggag gaactccccc tgcttccatc gttcgagatc gaccttcccg ctcttgagcg
     1981 cttccttgca aaagaacgga atgagcgaag cgaaggccgg ctgaagctcc tctttctctg
     2041 ttcgccgaac aacccgaccg gcaacagcta ttcccttgaa acgatcgaag cggtcttgaa
     2101 gatcttcgac ggcctggtgg tccttgacga ggcgtactac gatttcagcg aaggggagag
     2161 cggggtttcg cttctttcca agtatgacca cttagtggtt cttcgaaccc tctccaaagc
     2221 gtggggactt gcaagcgccc gggtcgggat cgccgtcgga agccgggagc tcatcgaggt
     2281 gatcaacaag atcaaatatc cctataacct cggaacccct tctcaggccc tcgccctcag
     2341 ggcgctggaa aacgccgagg cggtcaaaag aagccttgat gagatcaaga gggagcgctc
     2401 ccgtctttcc ggagcgcttg ctgcgtttgg ttgcgtgcgg atcgtgtatc cgagcgacgc
     2461 gaacttcctg ctgctcaaag tggatgacgc gacccggctc tataccttcc tgacggagaa
     2521 cggtacgatt atccgtaatc ggacgaagga gcgggggctg gagaactgcg tccgcatcac
     2581 ggtcggcagc caagccgaga acgatatcct ccttgagggg tttgaagcgt atatgaggaa
     2641 gtattatgca taagaagatc ttgttcgtgg atcgcgacgg ggtgatcgtc gttgaagacc
     2701 aggtcgattc cttcgagaag atccgctata ttccgggagt cttcggaggg ttgaaacggc
     2761 tggtggattc caatcagttt tatctcgtgc tggtctccaa tcaggacggg gtgggaactc
     2821 cctctttccc cttggaggat tttgaaccga tccacacccg gatcatcgaa acgttgcggg
     2881 gggaggggat cctcttcgat gaggagcata tcgattactc gcttcccgca ga
//
LOCUS       DAOPUZ010000128         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_35060_length_2930_cov_2.738783, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000128 DAOPUZ010000000
VERSION     DAOPUZ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35060_length_2930_cov_2.738783"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKN79_02940"
     CDS             <1..313
                     /locus_tag="PKN79_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94750.1"
                     /translation="MELAAGVGVTYVPQNPGVENAYYALYGGKKVAVEEIPFLDAVLQ
                     SPIHLQMGIGTDFGPLGLRARYLIESNQTIESVMAKGRWWEMFVPNSGCFSLAVLLKM
                     S"
     gene            368..982
                     /locus_tag="PKN79_02945"
     CDS             368..982
                     /locus_tag="PKN79_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94751.1"
                     /translation="MKKVWILLIVLAVLVPAAVSASPFGLSLGVTGLYSDTFGQIAKA
                     MEAGDYDGFSNIDNYRFGADLRIKLLLAEIDAVAMFGSEDGATNISMLTTAGLSFDLF
                     GLLRIGAGVGPRFTVNIESDGSMWVKDSNGNQVDLENAGEAFMKSPLAYRLTADLKLG
                     KILLGLNYTLDTNYTFEEWEKFENIFNAPFDNGYMGVSVLFSFF"
     gene            1080..1289
                     /locus_tag="PKN79_02950"
     CDS             1080..1289
                     /locus_tag="PKN79_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94752.1"
                     /translation="MARKGFFSSLLSSLGVAVISFFILYFFFPNISNPYLGVAFKKPT
                     EIVDQESPSRLRQLFDQATSWLPFR"
     gene            1406..2089
                     /locus_tag="PKN79_02955"
     CDS             1406..2089
                     /locus_tag="PKN79_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ94753.1"
                     /translation="MLLEDGGHALVIDTGPEFRLQALKHKITHLDAVFYTHDHADHIN
                     GIDDLRSFSRHGPLRVYASRAVVETIRVRFPYALDADVVAGGRPHLLLTVIQPYETVT
                     VGTMNVTALPILHGGDEIFAYQIGSLGYVTDCSEVPQKSVEYLRGLDTLVVDALRRFP
                     HPSHFSLFEALGLISHVGPKHAYLTHLSHGFAHQKLEEELPPSVHVAYDGLVIPIGGE
                     NEKEKELYH"
     gene            2061..>2930
                     /locus_tag="PKN79_02960"
     CDS             2061..>2930
                     /locus_tag="PKN79_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNZ94754.1"
                     /translation="MKKKKSFIIDGYGLIYRSYYGLGRMLSSNGENISAVYGFFTTLL
                     KLIREEEIDYISVAMDSAGPTFRHELYEAYKANRDAAPEDLHSQVPHIEAILKALGIP
                     VLEHAGWEADDIIASLAHEATKDGIHTVMFTGDKDLLQLITDDVSALRPARKGESFYR
                     PMGKTEVKEEFGVSVEQIIDYLALIGDSSDNIPGVRGIGPKGAAKLLEEYTTLEGVYE
                     ALEKQTPTMRKRLEENKEAALLSRRLVILDSTLYQEVGIEWEDYRVDRIDWANSIPLF
                     QELGMRTIIGELER"
BASE COUNT          664 a          706 c          833 g          727 t
ORIGIN      
        1 gatggagctc gctgcggggg tgggtgtaac atacgttcct caaaaccccg gggttgagaa
       61 cgcctactat gcgttgtacg gcggaaagaa ggtggcggtc gaggagattc cgttccttga
      121 tgcggtgctt cagagtccga tccatctgca gatgggaatc gggaccgact tcggaccgtt
      181 gggactgagg gcccggtatc tgatcgaatc gaatcagacg attgaatcgg taatggcaaa
      241 aggcaggtgg tgggaaatgt ttgttccgaa tagcggatgt ttttctcttg ctgtcctttt
      301 gaagatgagt taaattttac acacgaagct tataaacaga cagtgtgtag cagaaagagg
      361 tgacagaatg aaaaaagtat ggattctttt gattgtgttg gcggttctcg ttccggcggc
      421 ggtgagcgca tcgccattcg gactgagtct cggtgtgacg gggctctaca gcgatacgtt
      481 cggacagatt gccaaagcaa tggaagcggg ggactatgac ggattctcca acatcgacaa
      541 ctatcgcttt ggagcggatc tcagaatcaa gcttctgctt gcggagatcg atgcggtcgc
      601 gatgttcggt tctgaagatg gtgcgacaaa catttccatg ttgaccaccg ccggtctctc
      661 cttcgatctg ttcggtctgc tgcggatcgg agccggtgtc gggccgcgct tcaccgtcaa
      721 catcgaatcc gacgggtcga tgtgggtaaa agactccaac ggcaaccagg tcgatcttga
      781 gaatgccggc gaagcgttca tgaagagccc tctggcatac cgcctcaccg ccgatctgaa
      841 gcttggaaag attcttctcg gattgaacta cacgctggat acgaactata cctttgaaga
      901 gtgggaaaag tttgagaaca tcttcaacgc tccattcgac aatgggtata tgggcgtctc
      961 cgtcctcttc tccttcttct aacagagtgt tcgcatctcc ttgctcaggc ccgtgaaaac
     1021 gggcctgttt cggtgtaaca ggactcgtaa gtgtggtata gtggttcaag gagttccaca
     1081 tggccagaaa aggttttttc tcttccctgc tttcctcttt gggtgtcgcg gtgatttcat
     1141 tcttcatcct ctattttttc tttccgaata tcagtaatcc ttatttggga gttgctttta
     1201 agaaaccgac ggagatcgtc gatcaggaaa gtccatcacg gctgcgtcaa ttgttcgatc
     1261 aagccacctc ttggctgccg tttcgatgac cgcttgctcg ttgacttttt tagggaccgg
     1321 aaccagtcat gggatcccgg tcattgggtg tacatgttcg gtatgcacct cgagcgacga
     1381 gcgggatacg cggctgcgct cgagcgtgct gcttgaggat ggcgggcatg cgctggtcat
     1441 cgataccgga ccggagtttc gacttcaagc gctgaaacac aagattactc atctggatgc
     1501 ggtcttttac acccatgacc atgccgatca tatcaacgga atcgatgatt tgcggtcctt
     1561 cagcagacat ggtccgctgc gggtctatgc gagcagggcc gtggtggaaa cgattcgtgt
     1621 tcgttttccc tatgcgcttg acgcggatgt cgttgccgga ggacggcccc atctgttgct
     1681 gaccgtcata cagccgtatg aaaccgttac ggtgggaacg atgaacgtaa cggcacttcc
     1741 gattcttcac ggcggtgatg agatttttgc ctatcaaatc ggttcgctcg ggtatgtaac
     1801 cgactgtagt gaagttcccc aaaagagtgt agaatatttg cggggtcttg atacgctcgt
     1861 cgtggatgcg ttgaggcgct ttccccaccc atcccatttc agcctctttg aggcgttggg
     1921 gttgatcagt catgtgggac cgaagcacgc ctatcttacg cacctcagtc acggctttgc
     1981 ccatcagaag ttggaggagg agcttccccc ttcggtgcat gtcgcctatg atgggttggt
     2041 catccccatt ggaggggaaa atgaaaaaga aaaagagctt tatcattgac ggatatgggt
     2101 tgatctatcg ctcctattac ggtcttggga ggatgctctc ttcaaatggg gagaacatca
     2161 gcgccgtcta cgggtttttt acaaccctcc tgaagctgat tcgggaggag gagatcgatt
     2221 acatctccgt tgcgatggac agcgccggac cgacgtttcg tcatgaactc tatgaagcgt
     2281 acaaagcgaa tcgtgatgcc gccccggaag atcttcactc tcaggtgccc catatcgagg
     2341 cgattctcaa agctctcggc atccccgtcc tcgaacatgc cggctgggag gcggatgaca
     2401 ttatcgcctc gcttgctcac gaagcgacaa aggatggaat ccatacggtg atgttcaccg
     2461 gtgataaaga cctgcttcaa ctgattaccg atgatgtctc agccctcaga cccgcccgaa
     2521 aaggggagtc gttctatcga ccgatgggaa aaaccgaggt caaggaggag ttcggggtct
     2581 ccgttgaaca gatcatcgac tacctcgcct taatcgggga cagctcggac aacatccccg
     2641 gggtaagggg aatcgggccg aagggtgcgg cgaagcttct tgaggagtac acgacccttg
     2701 aaggagtgta tgaggcgctc gaaaaacaga ctccgacgat gaggaagcgg ctcgaagaga
     2761 acaaagaagc cgctctcctc agtagaaggt tggtaatcct cgattccacg ctttatcaag
     2821 aggtaggaat cgaatgggag gactatcgag tagatcggat cgattgggcg aattcaattc
     2881 ccctgtttca agagctcgga atgcggacaa tcatcggtga actggagcgc
//
LOCUS       DAOPUZ010000129         2926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_35118_length_2926_cov_2.947405, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000129 DAOPUZ010000000
VERSION     DAOPUZ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35118_length_2926_cov_2.947405"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PKN79_02965"
     CDS             <1..700
                     /locus_tag="PKN79_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNZ94755.1"
                     /translation="TLDSSIHPHILGRDHPYTIQTCDLHSVRGTFEGRPFTVHLGLEV
                     FATNTLLALLACSIITHKHPVELLPHLSNVRPLKGRMQAVPNDKGVHVFIDYAHTIGA
                     YERLFALIKQVRGASRVITVMGAAGGRDKKKRSVLGGIASKESDILILTEEDPVFEEL
                     SAINGMIRSGIASKACRVLEIDDRKEAIGEAIRLAKAGDIVLLLGKGHEQSIQRRNLT
                     IPWDDEQALLEVIR"
     gene            697..1755
                     /locus_tag="PKN79_02970"
     CDS             697..1755
                     /locus_tag="PKN79_02970"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNZ94756.1"
                     /translation="MNVALIYGGRSAEHEVSINSAKTIEKILKHNHHILYPIAIDRNG
                     CWFLQKQVQNTIDEEIPLWIVPGSGIYAQSGMLPIDVGFPTTHGRGGEDGNLQGLCTL
                     AKIPLAGCDTVSSAIGMHKALAQSVFDRAGIPTIPTIIVTEAMPVTDSLLEKLRQSLG
                     SSLFVKPENAGSSVGITVLIEADLPSLARALRLALSYSERALVQPYYPEVIEAECAVF
                     EDPEQGIIACGPGVIKNPKKETFLSYSQKYTLKGGAYLELPSGLSDEVEEEIRTLAVR
                     AFEAIKGNGYARVDFFLVDERPILNEINTLPGLTATSHWPKLLQYGGYSIERVVDLLL
                     EQAISDFDRRWNLRTSHD"
     gene            1748..>2926
                     /gene="dinB"
                     /locus_tag="PKN79_02975"
     CDS             1748..>2926
                     /gene="dinB"
                     /locus_tag="PKN79_02975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNZ94757.1"
                     /translation="MIETVIFHVDIDAFFAAVEILDNPDLAGLPLIVGGFSKRGVVTT
                     ASYEARKYGVHSAMPMIQALRLCPHATVVPTRRTRYSEVSARVMGILREFSSDVHQIS
                     IDEAFLDMSGTERLWGRPREAALKLKERVHEETGMTISVGIGPNRLIAKMASDYNKPD
                     GLCKVSESKKELFIDAVGLKKLWGVGKATQEELKSRRITTTGELRAIPIERMKKLFGN
                     SMGSFLYHVVRGIDPGIFSEAKSRSISTEHTFLEDVGDREVLEETLLSMSHEVMFRSL
                     DERKIARTIGIKIRLSDFSTSTVQTTPDEAIFSAEQVYAIAKELLAQKWRSGMKVRLI
                     GVGLYQLYDGASPIQGELFESEHEKKRKLEEVVLALQKEGHRLIKAANLGKHPLGDKD
                     Q"
BASE COUNT          681 a          801 c          837 g          607 t
ORIGIN      
        1 aacattggat tcctccatcc acccccatat cctcggtcgt gatcacccgt atacaatcca
       61 aacctgtgat ttgcattccg tccggggaac atttgaagga cggccgttca cggttcatct
      121 tggacttgag gtctttgcga cgaacactct gctcgccctg ctcgcctgca gcatcatcac
      181 ccacaaacat ccggttgagc tgctgcctca tctatcgaac gttcgtcccc tcaaaggccg
      241 gatgcaagca gttcccaacg acaagggtgt tcatgttttt attgactatg cccatacgat
      301 cggagcctat gagcgcctct ttgctttgat caagcaggtt cgaggagcta gccgggtgat
      361 cacggtgatg ggggccgccg gcggtcggga caagaaaaag cgttccgttc tcggcggcat
      421 cgcttcgaaa gaaagtgata tcctgattct gaccgaggag gatccggtct tcgaggagtt
      481 gagcgcgatc aacgggatga tcagaagcgg gatcgcttca aaggcgtgca gggtgctgga
      541 gatcgacgat cgaaaagagg cgatcggaga agcgattcga cttgccaagg caggtgacat
      601 cgtcctcctg ctcggcaaag gtcatgaaca gtcgattcag cgcaggaacc tgacaatccc
      661 ctgggatgac gaacaagcgc tgttggaggt gattcgatga acgtagccct gatctatggc
      721 ggcagatccg ccgaacatga ggtgtcgatc aacagcgcaa aaacgattga aaagatcctg
      781 aagcacaatc atcacatcct ctatccgatt gcgatcgatc gaaacggctg ctggttcctc
      841 caaaaacagg ttcagaacac gatcgatgaa gagattcctc tttggatcgt tcccggaagc
      901 gggatatacg ctcaaagcgg catgcttccg attgatgtag gctttcccac cactcacggc
      961 cggggcggcg aggatggaaa cctccaaggg ctttgcaccc ttgccaagat tcccttggct
     1021 ggctgcgata ccgtctcctc ggcaatcggg atgcacaagg cgttggccca atcggtcttc
     1081 gatcgggccg gcatacccac gattccgacg atcatcgtca ccgaagcgat gccggtcacc
     1141 gactcgcttc tggagaagct tcgacaaagc cttggttcga gtctcttcgt aaagccggag
     1201 aacgccggat cgtcggtggg gatcaccgtt ctcatcgaag ccgatctgcc aagccttgcc
     1261 cgggcccttc gcctggccct ctcctacagt gagcgggcgt tggttcagcc ctattaccct
     1321 gaagtgatcg aagcggaatg cgccgtcttt gaagatcccg aacaaggaat catcgcttgc
     1381 ggaccggggg tgatcaaaaa cccgaagaaa gaaacgttcc tctcctacag ccagaagtat
     1441 accctcaaag gcggagcgta ccttgagctt ccctccgggt tgagtgatga agtcgaagag
     1501 gagattcgca ccctggcggt tcgggcgttt gaggcgatta aaggcaatgg atatgcccgg
     1561 gtggatttct tcctcgtcga cgagagaccg atcctcaatg agatcaatac gctgcccggg
     1621 ttgacggcaa ccagtcattg gccgaagctt cttcaatatg gcgggtattc gattgagcgc
     1681 gtcgtcgacc tccttttgga gcaggcgatc tcagatttcg atcgaagatg gaatctgagg
     1741 acgagccatg attgagaccg tcatcttcca cgtcgacatt gatgcgttct tcgccgcggt
     1801 cgagattctc gacaaccccg acttggcggg actacccctg atcgtcggcg gcttttccaa
     1861 gcgcggcgtc gtgacgacgg caagctatga ggcgcgaaag tacggagtcc actcggcgat
     1921 gccgatgatt caggccctcc gcctctgccc tcatgctacg gtggttccaa ctcgcagaac
     1981 ccgctacagt gaagtctccg cccgggtgat ggggattctt cgggagtttt cttccgacgt
     2041 ccaccagatc tcgatcgacg aggcgttcct tgacatgagc ggaaccgaac gcctctgggg
     2101 acggccccgg gaagccgccc tgaagttgaa ggagcgggta cacgaggaaa ccggcatgac
     2161 gatcagcgtg gggattggtc ccaaccggct gatcgccaag atggcgagtg attacaacaa
     2221 accggatgga ctgtgcaagg tttccgaatc gaagaaggag cttttcatcg acgccgtggg
     2281 gttgaaaaag ctttgggggg ttggaaaggc gactcaggag gagctgaaga gccgaaggat
     2341 caccacgacg ggcgagcttc gagcgattcc gatcgaacgt atgaagaagc tcttcggcaa
     2401 ctcgatgggc tccttcctct atcacgtcgt tcggggaatc gacccgggaa tcttcagtga
     2461 agcgaaaagc cggtcgatct caacggagca caccttcctt gaggatgtcg gcgaccgtga
     2521 ggtattggag gaaacgcttc tttcgatgag ccatgaagtg atgttccgct cgctggacga
     2581 acggaagatc gctcgaacaa tcggaataaa aatccgactt tccgacttct cgacttccac
     2641 tgttcaaacg acccctgatg aagcgatctt cagcgccgag caggtctacg cgatcgcaaa
     2701 ggagctcctc gctcagaaat ggcgatcggg aatgaaggtc cgcctgatcg gtgtcgggtt
     2761 gtatcagctg tacgacggcg cttccccaat ccaaggcgag ctctttgaaa gtgaacacga
     2821 aaagaagcgg aagctggaag aagtcgtcct cgccctccag aaagaggggc acaggctcat
     2881 caaagcggcc aacctaggca aacaccccct cggcgataag gatcag
//
LOCUS       DAOPUZ010000130         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_35402_length_2908_cov_3.653347, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000130 DAOPUZ010000000
VERSION     DAOPUZ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35402_length_2908_cov_3.653347"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1022)
                     /locus_tag="PKN79_02980"
     CDS             complement(177..1022)
                     /locus_tag="PKN79_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ94758.1"
                     /translation="MNLNDAFPPWTKGCYQDKAPVRTCAKPFTLTRAKAKNAALLIHG
                     YAGYPGELIRVGRELFEEGLDVYVPRLPGNGTSGGDFQKSRAKDWIGTLRNGMDDLTK
                     KYDDVLIAGHSMGGALAVTLAKEYSVSNVILIAPGLLIPFVPVFKLRLIAPFIKKIAV
                     PWKPDPEYTFYYESEEDDDQYLGTEYWSWAFPKTLLELEKVRKEAVSNLSDLKSNTLL
                     ITGGKDSIIPYDVVALVKEKIQGPFTHLHLEEAGHYIPYDKNKDAQEEAYRGMREFVR
                     KAYSV"
     gene            complement(1019..1912)
                     /locus_tag="PKN79_02985"
     CDS             complement(1019..1912)
                     /locus_tag="PKN79_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNZ94759.1"
                     /translation="MSGFLLLIYMLAFALGSMTLALAIVYRMAHKERWASFFIVCHAS
                     LLGAMMLLALQTFTKLFITGFGGQVFSLILRIVVLADAAFLIVFLPFFTSWVIAHPWR
                     QPYAALFPFLAAVYLGLGIVNQIRPLLFFEQAQFVLFVFVIGFCLVVLVRNLGSIRNK
                     IARTSALTIIIVSLSMVPAIMLALFFPGFKPFLYAVYFLALSITIMVFLFMEFVRLGR
                     EEKQHTRQLTVDDLAPYNITEREFEIITLISQGLTNKEIASELDISANTVTNHVANIF
                     SKTQVRSRIDLLNIVKQSLYQ"
     gene            complement(1917..2555)
                     /locus_tag="PKN79_02990"
     CDS             complement(1917..2555)
                     /locus_tag="PKN79_02990"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB domain-containing protein"
                     /protein_id="HNZ94760.1"
                     /translation="MARKRLSQPPRKTILLVTASEIESLYFSQMRKDCRYSNMTVEHQ
                     PVFKDLHHLINLAARKRSAGGFTTVWLVFGLADLGLSAHDVRAELEHAEKKRVHLAWN
                     NPGLPLWFYLHFQEPKAFVRDMGAINGALEKAIDGYSYTSEYFLNDGDNFHLRLYSAK
                     SKAAVNGAMYNDRTRMEAGLEPTRMIALLNEITDICGLADLSRNQKQLGLKK"
     gene            complement(2558..>2908)
                     /locus_tag="PKN79_02995"
     CDS             complement(2558..>2908)
                     /locus_tag="PKN79_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94761.1"
                     /translation="FSSPRCLVIDDFGMLLHPNVLTHIVEIFEANNDENGSQMLVVDC
                     NPSLLKPGLLRRDGVYFAQKDSESATVYTSLANFRYSKANDRTQIQYMNGAFGALPIL
                     SEFSYDRESAQDKE"
BASE COUNT          697 a          756 c          807 g          648 t
ORIGIN      
        1 agaatgccct tgatttttcc aagcttatcg aagatgttgc ggataaaacg gaagatgatc
       61 ccacataaga cgagttcggc gggccagcct gtaaaattcg tcttcggatt tctcggtgcc
      121 tcgaatataa gaattccgag agtaatcgaa aatagattat tccatagggc accaattcaa
      181 acgctataag cctttctgac aaactccctc atccccctgt acgcctcttc ttgggcatcc
      241 ttgttcttgt cgtaggggat gtagtgtccg gcttcttcaa ggtggagatg ggtgaagggg
      301 ccttggatct tctccttgac cagggcgacc acgtcgtagg ggatgatcga atccttgcct
      361 ccggtgatga ggagggtgtt tgatttcaga tccgaaaggt tactcaccgc ttccttgcga
      421 accttctcaa gctccaggag ggtcttggga aaggcccagg accagtattc ggttccaagg
      481 tattggtcgt catcctcttc gctttcgtag tagaaggtgt actctggatc gggcttccaa
      541 ggaaccgcga tctttttgat aaacggtgcg atcaggcgaa gcttgaagac gggaacgaag
      601 ggaatcagaa gcccgggggc gatcaggatc acattggaaa cggaatactc tttggcaagg
      661 gtgaccgcca aggcgccgcc catcgagtgg ccggcgatca ggacatcatc gtactttttc
      721 gtcagatcat ccatcccgtt tctcagggtt ccgatccagt ccttcgccct gctcttttgg
      781 aaatccccgc cgctggttcc gttgccggga agccgcggaa catagacatc gagcccctct
      841 tcgaaaagtt cccgccccac cctgatcaat tcaccgggat agccggcata tccatggatg
      901 agcagcgccg cgttctttgc tttggctcgg gtcaatgtaa agggcttggc acaggtgcga
      961 accggcgcct tgtcctgata gcatcctttg gtccaggggg gaaacgcatc attaaggttc
     1021 attggtacaa actctgcttt acgatgttga gcaggtcgat ccgggatcgg acctgcgtct
     1081 tggagaagat attggccaca tggttggtaa ccgtgttggc gctgatatcc aattcacttg
     1141 cgatctcctt gttggtgaga ccttggctga tcagggtgat gatctcgaac tcccgttccg
     1201 tgatgttgta gggggcgaga tcgtcgaccg tcagctgccg ggtatgctgc ttctcctccc
     1261 ttccgaggcg gacgaactcc atgaacagaa agaccatgat cgtaatcgaa agcgcaagga
     1321 agtagaccgc gtagagaaac ggtttgaacc cggggaagaa cagggcgagc atgatcgccg
     1381 gaaccattga aaggcttaca atgatgatcg tcagcgcgct tgtccgagct atcttgttcc
     1441 ggatcgaacc gagattccgg acaaggacca ccagacaaaa gccgatcacg aacacgaaga
     1501 gaacgaattg cgcttgttca aagaacagca gcggacgaat ctgattgacg atccccaacc
     1561 caaggtagac agccgctagg aacggaaaga gcgcggcata gggctggcgc cacggatggg
     1621 cgatcaccca cgaggtgaaa aagggaagaa acacgatcag gaacgccgcg tcggcaagaa
     1681 caacgatcct gagaatcaag gaaaagactt gtcctccaaa gccggtgatg aagagtttgg
     1741 tgaaggtttg gagcgccaaa agcatcatcg ctccaagcaa cgaggcatgg cagacgatga
     1801 aaaagctggc ccaccgctcc ttgtgggcca tgcgatacac gatcgccaac gccagcgtca
     1861 tggacccgag ggcgaacgcc agcatataga taagcagcag gaaaccactc acggcgtcac
     1921 ttcttcaatc ccaactgttt ctggtttcgg gacagatcgg caaggccgca gatatcggtg
     1981 atctcattaa gcaaagcgat catcctcgtc ggctccaaac cggcttccat tcgggtccgg
     2041 tcattgtaca tcgccccgtt gaccgccgct ttgctctttg ccgagtacaa tcgaaggtgg
     2101 aaattatcac catcgttgag gaagtactcg ctggtatagg agtatccgtc gatcgccttc
     2161 tccagcgctc cattgatcgc ccccatgtcc cggacgaacg ccttgggctc ctggaagtgg
     2221 agatagaacc acaggggaag accggggttg ttccacgcaa ggtgaacccg cttcttctca
     2281 gcatgttcaa gctcggcgcg gacgtcgtgg gcgctgagac ccaaatcggc gagtccgaag
     2341 accagccaga cggtggtgaa acctccggca cttcgcttgc gggcggcgag gttgatcaga
     2401 tggtgcaaat ccttaaacac cggctgatgt tcgacagtca tgttggaata gcggcaatcc
     2461 ttacgcattt gtgagaaata cagactctca atctcactgg cggttaccaa taagatcgtt
     2521 ttgcgagggg gttgggaaag acgtttgcgt gccataacta ctccttatct tgtgcactct
     2581 ctcggtcata ggaaaattcc gagaggatcg ggagagctcc gaatgctccg ttcatatact
     2641 gaatctgggt tcggtcgtta gcttttgaat atcggaagtt cgccaaactg gtgtacactg
     2701 tagcgctctc gctgtccttc tgggcgaagt aaacaccgtc gcggcggagc aagcccggct
     2761 tgagcaggga cgggttacag tcaacgacca gcatctgcga accgttctca tcgttgttcg
     2821 cctcaaaaat ctcgacgatg tgcgtcagta cgttcggatg gagcaacatg ccgaaatcgt
     2881 ctatcaccaa gcagcgcgga ctggagaa
//
LOCUS       DAOPUZ010000131         2895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_35629_length_2895_cov_2.642958, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000131 DAOPUZ010000000
VERSION     DAOPUZ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35629_length_2895_cov_2.642958"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PKN79_03000"
     CDS             complement(<1..820)
                     /locus_tag="PKN79_03000"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNZ94762.1"
                     /translation="MSTTLRKTGVLLHPTSLPSKYGIGSLGKEALQCIDLLEKTGIRL
                     WQILPLGPTGYGDSPYAARSSFAGNELLIDLEELMVDGYLEYEDIYTVPVFNKDRIDY
                     QAVRAYKEPLLAQAAGSFLKHANKFERSEYKRFCKDHASWLEDYALHNVLCAVYNDSR
                     WFEVWPKELGLREPEALAAARKQYSAEIEEVKVLQFFFYRQWLKVRSYANERDIEIIG
                     DIPIFVAPDSADAWANRHLFKFDEDGKQLASSGVPPDFFSETGQMWGNPVYDWPV"
     gene            980..>1275
                     /locus_tag="PKN79_03005"
     CDS             980..>1275
                     /locus_tag="PKN79_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNZ94763.1"
                     /translation="MITKIEEIDFRGVDTLYFVIYLDISDMTVRIPVDKYQDMGIRPI
                     VGKEESQAAIDSISSKYEPMPVDWKARYQMNVDLLKEGSIASIAKVVQALYHRS"
     assembly_gap    1276..1375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1376..1566
                     /locus_tag="PKN79_03010"
     CDS             <1376..1566
                     /locus_tag="PKN79_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNZ94764.1"
                     /translation="GSIASIAKVVQALYHRSKIKELPVQERKLYDTALRLLIDECSIS
                     LEKDEKEIEMVVFSRLES"
     gene            1570..2379
                     /locus_tag="PKN79_03015"
     CDS             1570..2379
                     /locus_tag="PKN79_03015"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HNZ94765.1"
                     /translation="MAFPSHAVIVTAAGSSDRFNESKEIGVKKEYLNLGGHTVLYQSV
                     RPFLGIPNCRAIVVTHPEGMEDQCAVALEDLLEQNLVPIILVKGGKNRQESVLNGLEL
                     LATLSLPIKYVAIHDGARPYVSEELIITTLATATIFGGAVPALSTTDAIKTIDEHGQI
                     IGHSNRQQTVGVQTPQIFRYPEILEAHRRAAGTTNTYIDDTEIFTDYNQWVGICEGER
                     TNIKITYLSDIPDAEEQIKRYLADLEAGKKQARINEAFGSALSQAKPRRMG"
     gene            2376..2852
                     /gene="ispF"
                     /locus_tag="PKN79_03020"
     CDS             2376..2852
                     /gene="ispF"
                     /locus_tag="PKN79_03020"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNZ94766.1"
                     /translation="MRIGSGWDIHRLKEGATLVLGGVIIPSAKGLVAHSDGDVLIHAI
                     IDAILGALAKGDIGTHFPDTDPRFKGASSVDLLDHVVTKLLPPYSIGNIDTTIILKEP
                     KLRPHIDSIRYSLADILEVPIDHVSVKAKTAEGVLGELGTGDAIEAHATVLLTIKG"
BASE COUNT          678 a          737 c          754 g          626 t
ORIGIN      
        1 ggaccggcca atcatacacc ggattgcccc acatttgccc ggtttcgctg aaaaaatccg
       61 gcggtacacc actggatgcg agttgcttgc catcctcgtc aaacttgaag agatggcggt
      121 tcgcccatgc atcggcgctg tcgggggcga cgaagatcgg gatatccccg atgatttcta
      181 tatcccgctc gtttgcatag gaacggacct tcagccactg tcggtagaaa aagaattgaa
      241 ggactttcac ctcttcgatt tcggcggaat actgtttccg agccgcggca agagcttcag
      301 gttcgcgcag gccgagctcc ttcggccaga cttcaaacca tcgggaatcg ttgtacactg
      361 cgcacaatac gttgtgcagg gcatagtctt ccagccatga tgcatgatcc ttgcaaaaac
      421 gcttgtactc cgaacgttcg aacttgttgg cgtgtttcag aaacgatccg gcggcttggg
      481 caagcaatgg ttctttgtat gcccgaacgg cttgataatc gatccgatct ttgttgaaaa
      541 cgggaacggt gtagatgtcc tcatactcca gatacccatc caccatcaac tcttcaaggt
      601 cgataagcag ctcgtttccg gcaaaggagg agcgggcggc atagggcgaa tcgccgtatc
      661 ccgtcgggcc gagcggaaga atctgccata gccggatgcc ggtcttctca agcaggtcga
      721 tacactgcag ggcttctttt ccaagagatc cgattccata tttggaagga aggcttgtcg
      781 ggtgaagaag caccccggtc tttcgtaatg ttgtactcat gaaagtacta tacactattg
      841 gctttgtcgt tggctatctc tcttttgtcg ccttgttgga agtcgaaatt tacggtatac
      901 taatgccatg agtttatcag aaaaaacgtt gcctttctca gtcggggagc atgtcgtata
      961 tccccttcaa ggtgtgggtg tgattacgaa gattgaggag attgatttcc gaggcgtcga
     1021 tacgctctac ttcgtcatct accttgatat ctccgatatg accgtcagaa tcccggtgga
     1081 taaataccag gatatgggaa tcaggccgat cgtggggaaa gaagaatcgc aagcggcgat
     1141 cgattccatc tccagcaagt atgagccgat gccggtcgat tggaaggcgc gctatcagat
     1201 gaacgtcgat ttgttgaaag aaggttcgat cgcttcgatc gccaaggtcg tccaagcgct
     1261 ctaccatcgc tccaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaggt
     1381 tcgatcgctt cgatcgccaa ggtcgtccaa gcgctctacc atcgctccaa gatcaaggag
     1441 cttccggttc aggaacgcaa gctctacgac accgcgctgc ggctgttgat cgatgaatgt
     1501 tcgatctccc ttgaaaagga tgagaaggag atcgagatgg tcgtattcag tcgtttggaa
     1561 tcatgatata tggcatttcc atcccacgcg gtcatcgtta ccgctgccgg tagcagtgac
     1621 cgatttaatg aaagcaaaga gatcggagta aagaaggaat atctcaatct tggcgggcat
     1681 acggtcctct atcagagtgt ccgcccgttt ctgggcattc caaactgccg ggcgatcgtt
     1741 gttacccacc ctgaggggat ggaagaccag tgtgcggtgg cccttgagga cctgctggag
     1801 cagaacctcg tgccgatcat tctggtcaaa ggcggcaaga accgccaaga atcggtttta
     1861 aacggtcttg aactgctggc caccctctcg ctgccgatca agtacgtcgc gatccatgac
     1921 ggtgcccgcc cgtatgtgag cgaagaactg attatcacga cgctggcaac cgccacgatc
     1981 ttcggtggag ccgttcccgc tctctcgaca accgatgcga tcaagacgat cgatgagcat
     2041 ggacagatta tcggtcacag caaccgtcaa caaacggttg gagtacaaac tcctcagatc
     2101 ttccgttatc cggagatcct cgaagcccat cgccgggcgg ccggaactac caatacatat
     2161 atcgacgaca cggagatctt taccgattac aaccaatggg tcggcatctg tgaaggagaa
     2221 cggacgaaca tcaagatcac ctacctctcc gatattcccg atgccgagga acagatcaag
     2281 cgataccttg ccgacttgga ggcgggcaaa aagcaggcaa gaataaacga agcgtttggt
     2341 agcgcccttt cacaggcgaa gccgaggagg atgggatgag aatcggaagc ggctgggaca
     2401 ttcatcgtct caaagaggga gcgacgctcg tcctcggtgg agtaatcatc ccttcggcca
     2461 agggcctcgt cgcccacagc gacggggatg tgctgatcca tgcgatcatc gacgcgattc
     2521 tcggagcatt ggcgaaaggc gacatcggaa cccacttccc cgacacggat ccgcggttca
     2581 aaggggcatc ctcggtcgat ctcttggatc atgtggtaac aaagctcctt cccccctact
     2641 cgatcggcaa catcgacacc acgatcatcc tcaaggaacc gaagctccgg ccccacatcg
     2701 attcgatccg ctacagcctg gcggatatcc ttgaagtccc gatcgatcat gtgtcggtga
     2761 aggcgaagac cgccgaaggt gtactcggtg aactcggaac cggcgatgcg atcgaagcgc
     2821 atgcaacggt tctgttgaca atcaaggggt aaaaaactta tagtgtggcc tgcgcccaga
     2881 tggcggaatt ggtag
//
LOCUS       DAOPUZ010000132         2892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_35672_length_2892_cov_3.065915, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000132 DAOPUZ010000000
VERSION     DAOPUZ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35672_length_2892_cov_3.065915"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1234)
                     /locus_tag="PKN79_03025"
     CDS             complement(17..1234)
                     /locus_tag="PKN79_03025"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNZ94767.1"
                     /translation="MINIFDEEPARLHARYLAHLITKTGFLDFDSGKTLPVKEHGGVM
                     IGVLLGTDRYGNEVLLKAISGQQRNLARYVPHVITDTDFQTHLDTYDALIKNPHNTPF
                     NKSEYSAQALNHYYGLYRFPTIDGSTVSLFECFGNRPIPTGSGDCAAIKLLAYALGNH
                     IVPTSMTEFFYGDGTRSHLGFYQPCEERCAPILKRMLGLYPVYRDKHLLIVDKPPHLR
                     SIPGTVESDSIESRVRKLIPSAPVQCAAHRLDMDTSGLIVVALTKEALSGMHHLFRTQ
                     RVRRTYEALLEGILKEEETEITLPLRADITNRPYQVVDHEGGKEARTHVKRIRVEEHP
                     EGHLSRVEFTPYTGRTHQLRIHSKEGLGLPILGDRLYGSGMKSRLYLHAKSLAFIHPI
                     TKEELHIHAPVPF"
     gene            complement(1227..2570)
                     /locus_tag="PKN79_03030"
     CDS             complement(1227..2570)
                     /locus_tag="PKN79_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ94768.1"
                     /translation="MATQTRLMTAGSIRKHLIFFAFPIFLGGLFQQLYHTTDAVVVGN
                     FVSTQALAAVSSVGSLTMLIVGLFQGVFVGSGVVISSAFGQGDYDSVTKAVHTTVALG
                     IISSVLLTIIGYTFTPAILLWMKTPPEVFTDAQTYIQIFFLGVSSLVLYNTASGILQA
                     VGDSKNPLYFLIISTILNIVLDLVFVIFFGMGIEGTAYATIIAQSVSAILAFRLLIRT
                     KEVIQVRIKEIRIYRGYLGKTLRLGVPSGIQNSVTSFANVIVQSSINLFGPAAMAAHG
                     SYNRIQGFAFIPITAFALALTTFTGQNLGAGKQDRVRKAARFGIFFAMALAQLVGFAL
                     MIFGGQVMTIFTRDSEIIRLGVQKGLIAGWFFWALALSHTMTGIFRGAGKSIVPMTVM
                     LSIWCVLRIMFIKVGLALFFDIRIVFWAHPLTWTISSIIFIIYYFTADWMKRSDD"
BASE COUNT          713 a          698 c          842 g          639 t
ORIGIN      
        1 cccaccaacg gcaatcctaa aaggggacgg gtgcatggat gtggagttct tccttggtga
       61 tcgggtggat aaatgccagg ctcttggcgt gaagatacag gcggctcttc atccccgagc
      121 catagaggcg atcaccaaga atcggcagtc caaggccctc tttgctgtgg atccgcagct
      181 ggtgggtccg tccggtatac ggggtgaact cgacccggct gaggtgtcct tcaggatgct
      241 cctccacccg aatccgtttc acatgggtcc ttgcttcttt accaccctca tgatcgacga
      301 cttggtaagg ccgattggtg atgtcggccc gaagcggaag ggttatttcg gtttcctctt
      361 ctttaaggat tccttcaagc agcgcctcgt aggttcgtct tacccgttgg gtccgaaaga
      421 ggtgatgcat cccgctgagc gcttccttgg taagcgcaac gacgatgagg ccggaagtgt
      481 ccatatccag acggtgggct gcacattgaa ccggcgccga cgggatgagt tttcgtactc
      541 tgctttcaat cgaatcactt tcaaccgttc ccgggatcga gcggaggtgg ggaggcttgt
      601 cgacgatgag aagatgcttg tcccggtaca cgggataaag gccgagcatc cgtttaagaa
      661 tcggagcaca gcgttcctca cagggctgat aaaatcccaa gtgagagcgc gtgccgtcgc
      721 cgtagaagaa ctcggtcatt gacgtgggaa cgatatggtt gcccaacgcg taggcaagga
      781 gcttgattgc tgcgcagtca ccgctgccgg tcggaatcgg tcgattacca aaacactcaa
      841 acagggagac ggtactaccg tcaattgtcg gaaaccgata gagaccgtag tagtggttca
      901 gcgcttgggc ggagtattca cttttgttaa agggggtgtt gtgcggattt tttatcagtg
      961 catcgtaggt atcaagatgg gtttggaaat ccgtatcggt gatcacatgg ggtacatagc
     1021 gagcaagatt cctttgttgt ccgctgatcg ccttcagcag aacctcgttc ccatatcgat
     1081 cagttccgag caggaccccg atcatcaccc cgccatgctc cttcaccgga agcgttttgc
     1141 ccgagtcgaa atcaagaaag ccggttttcg tgatgaggtg ggcaagatac cgggcatgaa
     1201 ggcgagcagg ctcttcatcg aatatattaa tcatcgcttc gtttcatcca atcggcggtg
     1261 aagtaataga tgatgaagat gatcgaactg atcgtccagg tgagcgggtg agcccagaaa
     1321 acaatacgaa tatcaaagaa gagggcgaga cccaccttga tgaacatgat ccggagaaca
     1381 caccagatgg agagcatgac ggtcatcgga acgatcgatt tgcctgctcc gcggaagatt
     1441 cccgtcatcg tgtgactgag ggccagagcc cagaagaacc atccggcgat cagacccttt
     1501 tgcacaccga ggcggatgat ctcgctgtcg cgggtgaaga tcgtcatcac ctgaccgccg
     1561 aagatcatca gtgcgaaccc caccagttgg gcgagagcca tcgcgaagaa tatgccgaaa
     1621 cgggctgctt tcctcacccg atcttgtttt ccggcgccga ggttttgccc ggtgaacgtc
     1681 gtcagggcga gggcgaaggc ggtgatgggg atgaacgcaa agccctggat tcggttgtaa
     1741 gagccgtggg cggccatcgc cgccgggccg aagaggttga tcgatgactg gacgatcacg
     1801 tttgcgaagc tggtaacgga gttttgaatt cccgacggaa caccgagtct gagggtcttt
     1861 cccaaatacc cgcggtagat tctgatctct ttgatccgga cttgaatgac ctcttttgtt
     1921 cgaatgagca gtcgaaaagc gaggatcgcg ctgacgcttt gggcaatgat cgtggcatag
     1981 gcggttcctt cgattcccat cccgaagaag atgacgaaga ccaggtccag cacgatgttc
     2041 aggatggtgg agataatcag aaaatacagg gggtttttcg aatcccctac ggcttgaaga
     2101 atcccgctgg cggtattgta cagaacgagc gatgaaacac ccaaaaagaa gatctgaatg
     2161 taggtttgag catcggtgaa gacctccgga ggggtcttca tccagaggag gattgccggg
     2221 gtgaacgtat agccgatgat tgtcagcagg accgaactga tgatgccgag ggcgacagtg
     2281 gtgtgaacgg cttttgttac tgaatcgtaa tcaccctgac cgaacgcgga ggaaatgacg
     2341 accccggaac cgacgaagac cccttgaaag aggccgacga tcaacattgt cagcgatcca
     2401 accgaactga ccgcggcgag cgcctgagtg gagacgaagt ttccgactac cacagcgtcg
     2461 gtcgtatgat agagctgttg aaaaagccca ccgaggaaga tgggaaaagc aaagaatata
     2521 agatgttttc tgattgagcc ggcggtcatc aatcgcgtct gggtagccat aatagtcact
     2581 gtagacatct ttcatgaatg acgcaactac acattcttaa ttcatgtcga ttatgggcgt
     2641 atcaaagatc cggcccgttc agcggggtgc tgtcaatctt cagaaaacgg gggccccggc
     2701 tctagcttct tcgcatgtat gtgggtatct taaatcaaga tacggagggg ttcgtatgaa
     2761 actagagtta attcgtcatg tccatgtgtc tgaagagtac ttcaccggag cagggaagta
     2821 tcatcacggc aatcaagagg caatagaagt acagatgaag gctgtcgcca accaagggaa
     2881 ggcgaagtaa ta
//
LOCUS       DAOPUZ010000133         2886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_35782_length_2886_cov_3.685977, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000133 DAOPUZ010000000
VERSION     DAOPUZ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35782_length_2886_cov_3.685977"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1912)
                     /locus_tag="PKN79_03035"
     CDS             complement(<1..1912)
                     /locus_tag="PKN79_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNZ94769.1"
                     /translation="MKKKAGVKKTRGAIIAALAAFIWAGILVLPSFGIELPFMEMIFL
                     LFSFSTGDNPLWLLPSALFFLVASIILIVNRKRPIVLAFQLFYSALVTYTLHSLSHIL
                     QDSPWPIFRTYLLERTEGVRSASFTLVVFFIGEILLLIIIVLISDAIRHRRRPRLEEE
                     RVDEPVETETVTFVPKARAEFPNVDDLPEYVDRTPPPPKPKREKAPAAEEISGENTVS
                     LAMFRRLSEEVKKREEQQNKPSIPSPAKGKSRSRVDEKQGAKELPGLMLAAVKRMEER
                     KEEKVVEEKPRSFFARHSEPSTLEIEEKVPSPPPPPQMPQPEEPAFAAPPVVEHSENR
                     VSVKIPPQVEQPELFDEDDPLESSRGIGGLDASALISKPKIGYRFPSESILRTYEKIG
                     DVIDEHTLRQGQLLVETLLQFNIQVDVTKIVRGPTVTMFEVLPAPGIRVGSIVSLSDN
                     IALALAATQLRIVAPIPGKSAVGIEIPNKKRDIIGFKEMLGSVTEKMKIPMILGRTIL
                     DEKRVIDLSLSPHLLIAGSTGSGKSVCINSLICTILYRRTPKEVRMILVDPKIVELNI
                     YNGIPHLLTPVISDAKRTLKAFDFCLFEMERRYNLLRALNVRNIVGYNEKIAANNLAR
                     EKLPYIMVVIDEF"
     gene            complement(1927..2583)
                     /gene="rsmG"
                     /locus_tag="PKN79_03040"
     CDS             complement(1927..2583)
                     /gene="rsmG"
                     /locus_tag="PKN79_03040"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNZ94770.1"
                     /translation="MRRYDRLLDEGLARLDLFFDPEQRLRLDAYIAELELFNPVYKLV
                     GAQGEELVINHIFDSLAGVKTIEELAKRYDHPTLADLGSGAGLPGIPLAIALPNLDVV
                     LVERMGRRVDFLRTALVVTSLHRRVTIIDRDIRELNERFEVLTFRAFRPLVEILDSVA
                     PLLQVGGVVVAYKGLRANVEEELKEVKRKVTSRWEAELIDLDVPFLKRSRMLCLLHKI
                     "
     gene            complement(2576..>2886)
                     /locus_tag="PKN79_03045"
     CDS             complement(2576..>2886)
                     /locus_tag="PKN79_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNZ94771.1"
                     /translation="GKAIPELEAFPPSIRYQVELDVKYDGYIKRQERQINRFERFETM
                     TIPPGLDYDTLEGLSTEARQKLKHIRPLSVGQATRISGMRSSDAAILIIHLQRRSGDA
                     "
BASE COUNT          669 a          821 c          729 g          667 t
ORIGIN      
        1 cgaactcatc gatgacgacc atgatgtagg ggagcttttc ccgggccagg ttgttcgctg
       61 cgatcttctc attgtagccg acgatgttcc ggacattcag cgcccggagc agattgtacc
      121 gccgctccat ctcgaagaga cagaaatcaa acgccttgag cgtccgcttc gcatcggaaa
      181 tgaccggagt gagcaaatga ggaattccgt tgtagatatt gagctcgacg atcttgggat
      241 cgactaagat catccgcacc tctttgggag ttcgtctata gaggatggtg cagatcagac
      301 tgttgatgca cacactcttc ccgcttcccg tcgatccggc aatcaacagg tggggcgata
      361 aactcagatc gatcacccgc ttttcatcca gaatggttcg cccgagaatc atcggaatct
      421 tcatcttctc cgtgacgctg ccgagcatct ctttaaagcc gatgatatcc cgcttcttgt
      481 tgggaatttc aattccgacg gcgctcttgc cgggaatcgg ggcaacgatt ctcagttgcg
      541 tagcagcgag cgccaaggcg atgttgtcgc tgagactcac gatcgatccg actcgaattc
      601 ccggagcggg aagcacttcg aacatcgtta cggtcggacc gcggacgatc ttggttacat
      661 ctacctggat attgaactga aggagggttt caacaagcaa ctgcccttga cggagtgtgt
      721 gttcatcaat cacatctccg atcttctcat aggtacggag gatcgactca ctgggaaagc
      781 gatatccgat cttgggcttg gagatgagcg ctgaagcatc caagccccct attcccctgc
      841 tgctctccaa gggatcatcc tcatcaaaga gttccggttg ctcgacctga ggaggaatct
      901 tgacgctaac ccggttttcc gaatgctcca cgacgggagg agccgcaaac gccggttctt
      961 ccggttgagg catttgagga ggcgggggtg gtgaaggaac tttttcttcg atctccaagg
     1021 tcgagggttc gctgtggcgg gcgaaaaagg aacggggctt ttcctcgacg actttttcct
     1081 ctttccgttc ttccatccgt ttcacggcgg cgagcatcag gcccggaagc tctttggccc
     1141 cctgcttctc atcgacccgg ctcctgctct tccccttggc cggtgacggg atagacggtt
     1201 tgttctgttg ctcttcccgc ttcttcacct cttcggaaag acggcggaac atcgccaggc
     1261 tcaccgtgtt ttcaccgctg atctcctccg ccgccggtgc tttctcccgt ttgggtttcg
     1321 gaggcggcgg agtacggtcc acatactcgg gaagatcgtc aacattgggg aactcggccc
     1381 tcgccttggg cacgaacgta acggtttccg tctctaccgg ctcgtcgacc cgttcctctt
     1441 ccaaacgggg acgccttcga tgcctgatcg catcactgat cagcacgatg atgatgagaa
     1501 gcaggatctc gccgatgaag aacaccacca gagtaaatga ggcgcttcga acgccctccg
     1561 tacgctcaag cagataggtt cggaagatcg gccatggact atcctgaaga atgtgactca
     1621 ggctatggag cgtataagtg acaagggcgg aatagaacag ttgaaacgca agcacgatcg
     1681 gacgtttccg attcacaatg aggatgatgc ttgcaacaag gaaaaacaga gctgagggca
     1741 aaagccaaag aggattgtcc cccgtcgaga aggagaagag gaggaagatc atttccatga
     1801 atggaagctc aatgccgaag ctcggaagca caaggatacc ggcccagatg aaggccgcca
     1861 gcgcagcgat gatcgctccc cttgtttttt ttacacccgc tttctttttc atacccttcc
     1921 atcctatcaa atcttgtgca agaggcaaag cattcgactc cgcttcagaa acggaacatc
     1981 cagatcgatc aactccgcct cccatcggga agtgaccttc cgctttacct ctttcagttc
     2041 ttcttcaacg ttcgccctca agcctttgta cgccaccacc accccaccca cttggagaag
     2101 aggcgcaact gaatcaagaa tttcaaccaa aggccgaaat gcccgaaagg taagaacttc
     2161 aaagcgttcg ttcaactccc tgatatcccg atcgattatt gtaacacgcc ggtgcaagga
     2221 tgtgactacc aacgcggttc gaagaaaatc aactcggcga cccatccgct cgaccaacac
     2281 gacatccaga ttcggtagcg ctatcgcaag gggtatgccc ggcaagccag ctcccgatcc
     2341 caaatccgcc agcgtagggt gatcatatct ttttgcaagt tcttcaatcg tcttgactcc
     2401 cgccaatgaa tcgaaaatat ggttgatcac cagctcttct ccttgcgccc ccaccaactt
     2461 gtacaccggg ttgaacaact caagttcagc gatatatgca tccagccgta aacgctgctc
     2521 agggtcaaaa aagagatcta atcgagcaag gccttcatca agcagacgat cgtatctacg
     2581 catcgcctga ccgcctttgg aggtgaatga tgaggatggc ggcatcgctg gagcgcattc
     2641 cgctgattcg ggtcgcctgt ccgacgctca acggacgaat atgcttcagc ttctgccgtg
     2701 cttccgtcga cagcccttcc agcgtatcat aatccagacc cggaggaatc gtcatcgtct
     2761 caaagcgctc gaagcggtta atctgccgct cttggcgctt gatatacccg tcatacttca
     2821 catcaagctc cacctgatat ctgatcgatg gcgggaaggc ctccagttct ggaatcgcct
     2881 tgccaa
//
LOCUS       DAOPUZ010000134         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_35840_length_2883_cov_2.723126, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000134 DAOPUZ010000000
VERSION     DAOPUZ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35840_length_2883_cov_2.723126"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="PKN79_03050"
     CDS             complement(<1..1201)
                     /locus_tag="PKN79_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ94772.1"
                     /translation="MTEGLLDQEKSIGLIIWTLAWPAILEQILQVSVTYVDSAMVGSL
                     GAAATAAVSVPTSTIWLVNGWMNAFAIGYAVLMARNIGGRRFERARMITRQALVTALI
                     FGAVLSVAFTLIAQVLPAWIGAEEHVQTLARGYYTVIALAYLPNLLMITISSLMRLSG
                     DTRTPLLLNTLNNVLNIILNIFFIFGEVDLGPITIHGLGMGVKGAAIATSLAVTITAI
                     LLFIIWLTRPSIIQLELNQSFALNREIQKNAFRLGLPVGLERSTLSFGQIVLTKIVGS
                     LGTTALAAHFLANTAEAMTYLPASGVSTAATTLVAQSLGRGDKTLAKRFGNATTLIGT
                     LLMTTMGVVLYFTAPSLMGFFTRDQNVIALGTTILRIEAFVEPAFGLSMLVFGVFRGS
                     GDTRRPFY"
     gene            complement(1208..2206)
                     /gene="pta"
                     /locus_tag="PKN79_03055"
     CDS             complement(1208..2206)
                     /gene="pta"
                     /locus_tag="PKN79_03055"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNZ94773.1"
                     /translation="MLFAEKMKAQAKAAQKRLVLAEGSEERTIKAARIIADEHLASEV
                     ILIGSKAEIEKAASASSTSLEGLTLVDPATSDRRKQYATEFYELRKHKGMTEEQAFED
                     IANPLRFGAMMVRLGAGDAMVAGAENTTAAVLVAAFNIIKTAPGISFASSCFVMATDK
                     KEYGADGSFIFADCATIPNPTAEQLAEIALASAVSCSTFLGVEPKVGMLSYSTLGSAS
                     GELVEKVVEATKIVREKNPSLAIEGELQLDAAIVPSVAASKAPNSKVAGKANTLIFPD
                     LQAGNIGYKLVQRLAGAEAYGPILQGFAKPISDLSRGCSVEDIVVTSAITLAQAAK"
     gene            2301..>2883
                     /locus_tag="PKN79_03060"
     CDS             2301..>2883
                     /locus_tag="PKN79_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNZ94774.1"
                     /translation="MSEKNRVGVYGLGRFGLFWATRLAEKGFEVVGCSRRTVELPPNV
                     RQVSVEEVLDSPTVFYCVSISAFEEVLANSLPYLKEGAVVMDTCSVKLYPARVMKRLL
                     PPTIQAIATHPMFGPDSGRDGLEGLPFVMDNVSAHEETWRWWVDEFTSWKMDVLMMSC
                     DQHDREAAWSQGITHFIGRTLAELNLGSTRLATK"
BASE COUNT          586 a          794 c          890 g          613 t
ORIGIN      
        1 tatagaaggg acggcgggtg tcccccgaac cgcggaacac tccgaagacg agcatcgaga
       61 gaccgaacgc cggttcgacg aacgcttcga tccgcaggat tgtggttccc aaggcgatga
      121 cgttctgatc ccgggtgaag aatcccatca gggagggagc ggtgaaatag agcaccacgc
      181 ccatcgtggt catcaggagc gttccgatca gggtcgtcgc gtttccgaag cgctttgcca
      241 atgtcttgtc tccccggccg agcgactggg cgacgagggt cgtcgccgca gtggagactc
      301 cgcttgcggg cagataggtc atcgcctctg cggtgtttgc caaaaagtgg gccgccagcg
      361 cggtcgtgcc cagcgacccg acgattttgg tcaggacgat ctgtccgaag gagagagtgg
      421 agcgctcaag ccccacggga agcccgaggc gaaacgcatt tttttgaatt tccctgttca
      481 gagcgaatga ttgattcagc tccagctgaa tgatcgaagg ccgagtcagc cagatgatga
      541 agaggagaat cgccgtaatc gtaaccgcca agcttgtcgc gatcgcagcc ccctttactc
      601 ccatgcccag cccatgaatc gtgatcggac cgagatccac ttcaccgaaa atgaagaata
      661 tattgaggat aatgttcagc acgttgttga gggtgttcag aagaagcggg gttcgggtgt
      721 cgccgctgag gcgcatcaac gaactgatcg tgatcatcag gaggttggga aggtacgcca
      781 aggcgatgac ggtgtaatag ccgcgggcga gggtttggac gtgttcttcc gctccgatcc
      841 acgcgggcag gacttgggcg atgagggtga acgcgacgga aagaaccgct ccgaagatca
      901 gggctgtcac cagagcttgg cgggtgatca ttcttgcccg ctcgaagcgg cgccctccga
      961 tgttgcgcgc catcaacacc gcatacccga tcgcaaacgc attcatccat ccgttgacga
     1021 gccagatcgt cgaggtgggg acgctgactg cggcggtggc cgctgctccc agggagccga
     1081 ccatcgccga gtcgacatac gtgacggaga cctgaaggat ctgctccagg atcgccggcc
     1141 atgcaagagt ccaaatgatc aggccgatcg atttttcctg atcgagcagt ccctcggtca
     1201 tatgctccta cttggcggcc tgggcgaggg tgatggcgct ggtgacgacg atgtcctcaa
     1261 cggaacagcc cctggaaagg tcgctgatcg gtttggcgaa tccctgaagg atcgggccgt
     1321 acgcctccgc tcccgcaagg cgctgaacaa gcttgtagcc gatgttgccc gcctgaagat
     1381 cggggaagat cagggtgttc gcctttcctg cgaccttgga gttcggcgct ttggatgcgg
     1441 ccacggaagg aacgatcgca gcatccagct gcagctcgcc ttcgatcgcc aaggaggggt
     1501 tcttttcccg aacgatcttc gtcgcttcga ccactttttc gacgagctca ccgcttgccg
     1561 agccgagggt ggagtagctg agcatgccga ctttcggttc cacgccgagg aaggtggaac
     1621 agctgaccgc tgaagcgagg gcgatttcgg cgagctgttc ggctgtcggg ttgggaatcg
     1681 ttgcacagtc ggcaaagatg aacgagccgt cggctccata ctccttcttg tcggtagcca
     1741 tcacgaagca cgaagaagca aaactgatgc cgggggcggt tttgatgatg ttgaacgcgg
     1801 ccacaagtac cgctgcggtg gtgttctccg ctccggcgac catcgcatca ccggctccga
     1861 gacgaaccat catcgctccg aaccgaagag gattggcgat gtcttcaaaa gcctgctctt
     1921 cggtcatccc cttgtgcttg cgcagttcat agaactccgt cgcgtattgc ttgcgccgat
     1981 cgctcgttgc gggatcgacg agtgtcagcc cttcgagcga ggtggaggat gcgcttgccg
     2041 ctttttcgat ctccgccttg gatccgatga ggatcacctc gctggccaag tgctcgtcag
     2101 cgatgatccg agcggctttg atcgttcgct cttcacttcc ttccgccagg acaagccgtt
     2161 tttgagcagc ttttgcctga gccttcatct tctctgcaaa taacataatt catgctccta
     2221 aaatgtggta tcgatgctca atcagtgtac cacgtccggc aatcttggag aagactcgtg
     2281 aattctgtac aatacccttc atgagtgaaa agaatcgggt tggagtatat ggtcttgggc
     2341 gcttcgggct cttttgggcg acccggcttg ccgagaaggg gtttgaggtc gtcggctgca
     2401 gccggcggac cgtggagctg ccgccgaatg tccgccaagt gagcgtcgaa gaggtgttgg
     2461 attctccgac cgttttttat tgtgtctcga tcagcgcgtt cgaagaggtt cttgccaaca
     2521 gcttgcccta tctgaaggaa ggggcggtcg tcatggacac ctgttcggta aagctgtatc
     2581 ccgcccgcgt gatgaagcgc ctcctccctc cgacgattca ggcgatcgca acccacccga
     2641 tgttcggtcc ggattcggga agggacggac tggaaggact gcccttcgtc atggacaatg
     2701 tcagtgctca cgaggagacc tggcgatggt gggtggatga gttcacctcg tggaagatgg
     2761 atgtgcttat gatgagctgt gaccagcacg accgtgaagc ggcgtggagc cagggaatca
     2821 cccacttcat cggccgaacg cttgccgagc tgaatctggg atcgacccgc ttggcgacca
     2881 aag
//
LOCUS       DAOPUZ010000135         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_35885_length_2880_cov_3.495929, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000135 DAOPUZ010000000
VERSION     DAOPUZ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35885_length_2880_cov_3.495929"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PKN79_03065"
     CDS             <1..220
                     /locus_tag="PKN79_03065"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HNZ94775.1"
                     /translation="ETAESAVTLIEESGLQPRDLINRVSSAKKTTVEGIKALNDGNLQ
                     ATVMDAVRKTAQRSRELEEAAANHTERK"
     gene            228..1502
                     /gene="serS"
                     /locus_tag="PKN79_03070"
     CDS             228..1502
                     /gene="serS"
                     /locus_tag="PKN79_03070"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNZ94776.1"
                     /translation="MIDLRELKSRYDEIITNIKNRSMKIDLDAIIALQDQRSSLLQEV
                     ESLRYERNENAQKMKGKLDQETRARLIEEGKNLKAKIAETEERFNRVEQEYLLLGRQI
                     PNYAHPSAPVGKEDTDNLILKRVGTPPTFTFKPLDHVELGEKLDLIDFEAGTKVTGPK
                     FYFLKNEAVILQMALERYALDILIKHGFAPYITPDIAKEEILEGIGFNPRGPESNIYT
                     LEGTGTALVGTAEITLGGYHAEEILDINRLPIKMAGLSHCFRREAGSAGQFSKGLYRV
                     HQFSKVEMFIYCLPEESDAIHEQLLAIEEEIFSGLGLAYQVVDTCTGDLGAPAYRKFD
                     IEAWMPGRGENGEYGEVTSTSNCTDYQSRSLRVRYKDADGQNQYVHTLNGTAVATSRA
                     IVAILENFQEEDGSIRIPAVLVPYTGFDRIGG"
     gene            1506..>2880
                     /locus_tag="PKN79_03075"
     CDS             1506..>2880
                     /locus_tag="PKN79_03075"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNZ94777.1"
                     /translation="MFHRSALSTDFYELTMAQGYFLERNNPKVVFEMFYRTNPFGGGY
                     VLFAGLHDLIDKLEAFRFSPEDIAYLSGLGIFTKEFLEYLSKYRFTGDIYSVEEGTVV
                     FPGEPLMRVHTTMIDASLIEGILLNTLNFQSLIATKASRVSLAAKRGSVFEFGLRRAQ
                     GPDGALSASRAAFIGGCQFTSHTLAGKLYDIPVSGTMAHSWVMAFDSELESFRAYAKL
                     YPGSTILLIDTYDTLGSGIENAIIVGLEEKEKGNTIGVRIDSGDLSYLPRVIRKRLDD
                     AGLEDAFIVVSNDLTEEIIQTLVQDGVPIDSWGIGTHLVTGGTQASLNGVYKLAAKEL
                     RDGSYLPTMKISNSFEKTTNPGIKQLYRFYDAEGGAIADLVTCFDEEISVGNDYVFYH
                     PFAETEFFEMKKERYVRIEPLLNHVVKNGKRIGEKPCLKTLQARAIRSLDTIHKTYLR
                     QINPHIYK"
BASE COUNT          716 a          819 c          767 g          578 t
ORIGIN      
        1 cgagaccgcc gagagcgccg tcaccctaat tgaagaaagc ggattgcagc cccgagatct
       61 gatcaatcgc gtttccagcg caaaaaaaac gacggttgaa ggcatcaaag ccctgaatga
      121 cggaaacctg caggcgaccg tcatggatgc ggtcagaaaa accgcccaac gatcgagaga
      181 gctcgaagaa gcagcagcaa accatacaga gaggaaatga ggataccatg attgacctac
      241 gggaactgaa aagtcgctat gacgaaatta tcacgaatat caagaaccgc tcgatgaaga
      301 tcgaccttga tgcgatcatc gccttgcagg atcagcgaag cagcctgctt caggaggttg
      361 agtccctccg ctatgagcgc aacgaaaacg ctcaaaagat gaaaggcaag ctcgatcagg
      421 aaacccgcgc ccgtctgatc gaagagggaa agaatctgaa ggcgaagatc gccgaaacgg
      481 aagaacgctt caaccgggtt gaacaggaat accttcttct cggccgacag attcccaact
      541 acgcccaccc ttccgccccc gtaggcaaag aagataccga caacctcatc ctcaaacggg
      601 tcggaactcc tccgaccttc accttcaaac ccctcgacca cgtcgaactc ggcgagaagc
      661 ttgatctcat cgacttcgag gcggggacga aggtgaccgg accgaagttt tacttcctca
      721 agaatgaagc ggtgattctt caaatggctc ttgagcgcta tgctttggac attctcatca
      781 aacacggttt tgctccttac atcacccccg acatcgccaa ggaagagatc ctggaaggga
      841 tcggattcaa tccgcggggg ccggagtcca acatctatac cttggaaggc acgggaacag
      901 cgttggtcgg caccgccgag atcaccttgg gcggttatca cgccgaggag attctcgaca
      961 tcaatcgcct cccgatcaag atggccggtc tctcccactg cttccgccgc gaggcgggtt
     1021 cggccggcca attctccaaa ggcctctacc gggttcacca gttctcgaag gtcgagatgt
     1081 tcatctactg tcttcccgaa gaatcggatg cgattcacga gcagcttctt gcgatcgaag
     1141 aggagatctt ctccggcctg ggtctcgcct atcaggtggt ggatacctgt accggggatc
     1201 tcggtgcccc ggcgtaccgg aaatttgaca tcgaggcatg gatgcccggc cgcggcgaga
     1261 acggcgaata cggggaagtc acctcgacaa gcaactgcac cgactatcaa agccgctccc
     1321 tgcgggttcg ctacaaggat gccgatgggc aaaaccaata tgttcacacc ttgaacggaa
     1381 ctgcggtggc taccagccgg gcgattgtcg ccattctgga aaacttccag gaggaggacg
     1441 gctcgatccg aatcccggcg gttctcgttc cgtacaccgg tttcgaccgg attggaggtt
     1501 aacggatgtt tcatcgctca gcgctcagta ccgatttcta tgaactcacg atggctcaag
     1561 gatattttct ggagcggaac aatccaaagg tcgtctttga gatgttttat cgaaccaacc
     1621 cgtttggagg cggatacgtc ctctttgccg gcttgcacga tctgatcgac aagcttgaag
     1681 cctttcgttt cagcccggaa gatatcgcat acctttcagg tctgggcatc ttcacaaaag
     1741 agtttctgga gtatctctcc aagtaccgct tcaccggtga catctactcg gttgaagagg
     1801 gaacggtcgt gttccccggt gaaccgctca tgcgcgttca cacgacgatg atcgacgcca
     1861 gcctgatcga gggaatcctg ctcaacaccc tcaacttcca aagcctcatc gccaccaaag
     1921 cttcccgcgt ctctcttgcc gccaagcgcg ggtcggtctt cgagttcggc ctgcgccgag
     1981 cccaaggtcc cgacggggcg ctttcggcaa gcagggcggc gtttatcggc ggatgccagt
     2041 ttaccagcca taccttggcg ggaaagctct atgatattcc cgtctcggga acgatggccc
     2101 attcatgggt gatggcgttt gattcggaac ttgaatcgtt tagggcgtat gccaagcttt
     2161 accccggttc cacgatcctc ttgatcgata cctacgacac cctcggcagc ggaatcgaaa
     2221 acgcaatcat cgtcggcctc gaggagaagg aaaagggaaa caccatcggc gtccggatcg
     2281 acagcggcga cctctcctac ctgccaaggg tgatccgcaa gcggctggat gacgccggac
     2341 ttgaggatgc gttcatcgtc gtttccaacg atctgacgga agagatcatc cagacgctgg
     2401 tgcaggacgg ggttcccatc gacagctggg gaatcgggac ccatctcgtc accggtggca
     2461 cccaggcatc cttgaacggg gtctacaagc tcgccgccaa ggagcttcga gacgggagct
     2521 atcttccgac gatgaagata tccaactcct tcgaaaagac gaccaatccc ggaatcaagc
     2581 agctctaccg cttctatgac gctgaaggag gagcgatcgc cgatctggtc acctgttttg
     2641 acgaagaaat ttcggtcgga aacgactatg tcttctacca cccgttcgcc gaaaccgaat
     2701 tcttcgagat gaagaaagaa cggtatgtgc ggatcgaacc gctcctcaat catgttgtga
     2761 aaaacggaaa acggatcggt gagaagccat gtctgaaaac ccttcaagca cgagcgatcc
     2821 ggagcctgga caccatccat aaaacgtatt tgaggcagat caacccgcat atctacaagg
//
LOCUS       DAOPUZ010000136         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_35932_length_2878_cov_4.034006, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000136 DAOPUZ010000000
VERSION     DAOPUZ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35932_length_2878_cov_4.034006"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1649)
                     /gene="dnaK"
                     /locus_tag="PKN79_03080"
                     /pseudo
     CDS             complement(1..1649)
                     /gene="dnaK"
                     /locus_tag="PKN79_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270787.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
     gene            complement(1724..2269)
                     /locus_tag="PKN79_03085"
     CDS             complement(1724..2269)
                     /locus_tag="PKN79_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNZ94778.1"
                     /translation="MSKKEEAKKKETVKKEDTEELVEDLQSIEDELAEARREIAALKE
                     ENLRDRADLENWRKRMLRDKEDSIRFANERLIIDLLGPLDDFERAIEAAEEAQDFSKV
                     HDGVVLVSEQIYSTLQKNWGLERIEAVGKEFDPNEHEAYQVVEDENLEHETVLEEYAT
                     GYKLHGRVLRPSKVKVGRPAI"
     gene            complement(2368..>2878)
                     /locus_tag="PKN79_03090"
     CDS             complement(2368..>2878)
                     /locus_tag="PKN79_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94779.1"
                     /translation="LMGLEIILSLLLFVITLIIMLLLRARDKRSHSLEVMKNRMGQFI
                     KEIDATRSLFREEMQSVEERLNWVIDEARRGSEALSSRLDEIDRRRSELIELESILVR
                     YRNTLLELGETTLAIDRRIEEQKTWQKQIDDIHAQIDEFDFRLQGFASSFERALKSAY
                     KAMEEPLLT"
BASE COUNT          561 a          845 c          725 g          747 t
ORIGIN      
        1 atctccatgt tcggagaggg atttctcggt ggaatacacc attaatccgc ttcattgcgg
       61 gcatctattc gagcccgttc ccgcttgtct tcttcggcgt gcagctccgc ttccttgacc
      121 atccgctcga tctccgcttc ggagaggccg ctggaggatt cgattcggat cttttgctcc
      181 ttgccggttc caaggtcctt tgccgaaacg tgaacaattc cgttcgcatc gatatcgaag
      241 gtcacctcga tctgaggcac accccgcggt gcggagggaa ttccgacgag gtcaaagttg
      301 ccgagcgtcc tgttctggga tgccatctcg cgctctccct gaaggacgtg gatgctgacc
      361 gcggtctgat tgtcggcagc ggtggagaag atctgactct tgcgagtcgg gatcgtcgtg
      421 ttccgctcga tgaggcgggt atttacgcct cccaacgtct cgattcccag agaaagcggg
      481 gtaacatcga gaagcaggac atccttcacc tttccgccga ggattccgcc ttggatcgcc
      541 gcacccatcg caacgacctc atcggggttc acacccttgt gcggctcttt tccgaagagc
      601 tccttgacca tcgcctgcac ggcggggatt ctcgtcgatc cgccaacgag gatgacctca
      661 tcgatatcac cggcgctcag gccggcatcg cgaagggcgt tctggacggg ctttctggat
      721 cgatcgatca gatcggcgat caattgctcg aacttggcgc ggctcaaggt catctgcagg
      781 tgcttcggtc ccgatgcatc cgccgtgatg aacggcaggt tgatatcggt gctcgttgag
      841 ttcgagagct cgatcttcgc cttctccgcc gcctcgcgca ggcgttgaag cgccatcttg
      901 tcctttgaga ggtcgatcgt cgcgctcttc ttgaactcat cgatcatcca ggagatgatc
      961 cgctggtcaa agtcgtcacc gccgaggtgg gtatcgccgt tggtggactt cacttcaaag
     1021 actccgtctc cgagttcaag gattgaaata tcgaacgttc cgccgccgag gtcatacact
     1081 gcgatgcgct cttcacggct ggcgtctttc ccgaggccat aggcaagggc cgcggcggtc
     1141 ggttcgttga cgatccgctt cacatccaag ccggcgattc gaccggcatc cttggtcgcc
     1201 tgtcgttgag cgtcgttgaa ataggcggga accgtgatga ccgcttcggt gatcgtctcg
     1261 ccaaggtaat cttcggcggt cttcttcatc ttctgcaaaa tcgccgcgct gatctccggc
     1321 gggctgtagg agttgccgcg aatatcgatc tgaatctcgc cccctgcggc ctccttcagc
     1381 ttgtagggaa tcaaattcac ctcggaggga atatccttat acttgcgccc catgaagcgc
     1441 ttgatcgaat acaccgtatt ttcggcgttc gttaccatct gattctttgc gggttgacca
     1501 acgaggcgat cgcctttgct ggtaaacgca accacgctgg gggttgtccg ctgtccttca
     1561 ctgttggaaa taacgaccga atcggttcct tccatcactg cgacgcagct attggtcgtt
     1621 cccaagtcga tcccaataat tctacccatt gtataactcc tctttatctc atcaattctg
     1681 tttcattccg tgtatgtaaa atactcatac accggcctta tcgtcaaatg gccggtcgtc
     1741 caactttcac ttttgagggg cgaagcaccc gaccatgaag tttatagccg gtggcatatt
     1801 cctccaagac cgtttcatgt tcaaggtttt catcttcaac gacctggtac gcctcatgct
     1861 cgttggggtc gaactccttt ccgaccgctt cgattcgctc caagccccaa tttttctgaa
     1921 gcgtcgaata gatctgttca ctgacgagga ccaccccgtc gtgcactttc gaaaagtcct
     1981 gtgcctcttc cgccgcttct atcgctcgtt cgaaatcatc aagcggtccg agaaggtcga
     2041 tgatcaggcg ttcgttggcg aatcggatcg aatcctcctt gtcccggagc attcgcttgc
     2101 gccaattctc cagatccgcc cgatcgcgga gattctcctc tttcagagca gcgatctccc
     2161 gtcgagcctc ggcaagttca tcttcaatgc tctgaagatc ttcaacaagc tcctccgtat
     2221 cttccttctt cactgtttct tttttcttcg cttcttcctt cttactcatc tacaggtcct
     2281 cgtattaccg attacaataa aatgcatata aaagcatggg accgattcgg cccacgcgac
     2341 taacatactc atcccctgct tgaagcgtca agtaagaaga ggttcttcca tcgccttgta
     2401 tgcgctcttc agcgcccgct cgaatgagga ggcgaatccc tgcagacgaa agtcgaactc
     2461 atcgatttga gcgtgaatat catcgatctg tttctgccac gttttttgct cttcgatcct
     2521 gcggtcgatc gcaagggtcg tttcgccaag ctccaggagg gtattgcgat agcggacgag
     2581 aattgattcc agttcgatca gctcactccg ccgccgatcg atctcgtcaa ggcgggaaga
     2641 gagcgcctcg ctgccgcgcc tggcttcatc gatcacccag ttgaggcgtt cttcgacgct
     2701 ttgcatctct tcccgaaaaa gggaacgggt ggcatcgatc tccttgatga actgccccat
     2761 ccgattcttc atcacttcca gagaatgact gcgtttatcc cgagcccgca agaggagcat
     2821 gatgatcagc gtgatgacaa agagcagtag cgacagtatg atttcaagcc ccatgagt
//
LOCUS       DAOPUZ010000137         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_36116_length_2868_cov_3.618557, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000137 DAOPUZ010000000
VERSION     DAOPUZ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36116_length_2868_cov_3.618557"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="PKN79_03095"
     CDS             complement(<1..1384)
                     /locus_tag="PKN79_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNZ94780.1"
                     /translation="MKRDPALFMALVTVWLSFAVFIFLPFLRLLGATFVVDGKPSFAN
                     VKTVLSKAYNVKALYGSLILATAVSITGTVVGYLFALAVTRTRISKVTKGIIGAVAML
                     PLISPPFTSSISLTLALGPNGMLLKALGIGDFSIYGFLGTWISETLTYFPVAFMVIAA
                     VLNTMSATLDDAALSLGASRWRMFRTLTIPLSLPAIVNSILLLFGASLADFATPLILG
                     GHAFPVLPTEAYLLITGMFDLKGGATMSFLLLVPALLVFFLQRMMLGKKSFVSVTGKS
                     GSRTEFARLPQSLNVVIAVVCLIVVAFIIYLYAIILWGSLVKVWSVNNTPTLEHYRYV
                     FKHGQKAIKDTVLIAIISTVIGSLLAVVIGFMIQRKEFVGRRLLEFSSMLNYALPGTV
                     VGIAYIIAFNTKPLVLTGTLSILVAAYVFRYYATGIRSVIASLEQIDPALEEASLSLG
                     AGTVRTFTHIT"
     gene            complement(1453..2469)
                     /locus_tag="PKN79_03100"
     CDS             complement(1453..2469)
                     /locus_tag="PKN79_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ94781.1"
                     /translation="MKKALIVLSMIGLLLTPVFAAGVQEKASTTLNVYTIMPEKYATT
                     VFEEFTKDTGIQVNFVRLSSGEALARLIAEKANPQVDALWGGPADTYEAGVLEGVFEV
                     YVPSEASAIPAQFKSSKGYWTGIGVIPLTFLSSNSFLKEKGLQPPTSWNDLLDPRYYN
                     ALQMADVRTSGTATERVYSLIQVWGEDKAYEYQKQLHKNIQLYTKSGAGGAMPIATGQ
                     AGSGIFYIVDALDIQQQGYDLVISYPVEGVTYGVEGSGIVKGAKNLDAAKKLMDWASS
                     KRLGQVMVDNKINYIPTRPDVVVTNPALDMSKVKLLETSVAWKGANRERLVQRWIDEV
                     IQGQ"
     gene            complement(2580..>2868)
                     /locus_tag="PKN79_03105"
     CDS             complement(2580..>2868)
                     /locus_tag="PKN79_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNZ94782.1"
                     /translation="DIRRRVLEIDGQEITLTPTEFRIIEYLALHAHVPVSAEELLKQV
                     WNAEEWIQGPEMVKVNISRLRKKIEPNPSTPRFLVNRWGFGYLLTDTQEVS"
BASE COUNT          675 a          803 c          791 g          599 t
ORIGIN      
        1 ccgtgatgtg ggtgaaggtt cttaccgttc cggctcccag actcaacgat gcctcctcca
       61 aggcgggatc gatctgctcc agggatgcga tcacggaacg gatgccggtc gcataatacc
      121 tgaagacata ggctgcgacg agaatcgaaa gggttccggt gagaaccaga ggtttcgtgt
      181 tgaacgcgat gatgtaggcg atacccacga ccgttccggg cagggcgtag ttgagcatgc
      241 tgctgaactc cagcaacctt cgaccaacga actccttgcg ttggatcatg aacccgatga
      301 ccaccgcaag cagggagccg atgacggtgg agatgatcgc gatcaggacc gtgtccttta
      361 tcgctttttg accatgcttg aagacatatc tgtagtgctc aagggtcggc gtgttgttta
      421 cgctccatac cttcaccaag gagccccaca ggatgatcgc atagaggtag atgatgaacg
      481 cgaccacgat caggcacacc acggcgatga ccacgttcag agactgggga agccgggcga
      541 actcggtgcg ggagccgctt tttccggtaa ccgatacgaa gcttttcttc ccgagcatca
      601 tccgctgaag aaagaagacg agcagcgcgg gaaccagcag aaggaacgac atcgtcgccc
      661 cgcccttcaa gtcgaacatg ccggttatga ggagatacgc ttcggtgggc aaaacgggga
      721 acgcatgtcc gccaaggatc agcggggtgg cgaaatccgc cagactggcc ccgaacaaca
      781 acagaatcga gttgacgatc gccggcagcg aaagcgggat cgtcaatgtc cggaacatcc
      841 tccagcggga agcccccaac gagagggcgg catcgtccag cgtagcgctc atcgtgttga
      901 gcacagccgc gatgaccatg aacgccaccg ggaaataggt gagcgtctca ctgatccatg
      961 ttccgagaaa cccgtagatg gaaaaatcac cgatccccag agccttcagc agcatcccgt
     1021 tgggtcccaa cgcgagggtc aatgaaatgc ttgaggtaaa gggaggtgaa ataagaggga
     1081 gcatcgctac ggcgccgatg atccctttgg taacctttga aattcgagtc cgggttacgg
     1141 caagggcgaa cagatacccg actaccgtcc ccgtgatgga gaccgccgta gccaggatca
     1201 ggctcccata gagggctttt acattatagg ccttggataa caccgttttg acattggcaa
     1261 acgacggctt gccgtcaacc acgaaggtgg ctccgagcaa ccggagaaac gggaggaaga
     1321 taaaaacggc aaaggagagc cagacggtga ccagcgccat gaacagcgcc ggatcgcgct
     1381 tcatcaatga actacgaact cttttgggga tctgtgcttc cacgttctta ccttcctctc
     1441 tctagcaggg cgtcactgac cttggatgac ctcatcaatc cagcgctgga ccaaacgttc
     1501 acggttcgca cccttccaag cgacgctggt ttcaagaagc ttcaccttgg acatgtcgag
     1561 agccggattg gtcaccacaa catcgggccg agtcggaata tagttgatct tattgtcgac
     1621 catgacctgt ccgaggcgct tggaagatgc ccaatccatg agcttcttcg ccgcatcaag
     1681 gttctttgca cccttcacga tgccggagcc ttcgacacca taggtgacac cttcgaccgg
     1741 atatgaaatc accagatcat acccctgttg ctggatatca agagcgtcaa cgatgtagaa
     1801 gatgccggaa ccggcttgac cggttgcaat cggcatcgca ccgccggctc cactcttggt
     1861 gtacagctgg atgttcttgt gcagctgttt ctgatactcg tacgccttat cctcgcccca
     1921 gacctggatc aacgaataga cccgttcggt cgccgttccg ctggttcgga cgtccgccat
     1981 ctgaagggcg ttgtagtagc ggggatcaag aagatcgttc cagctggtag gcggctgcaa
     2041 ccccttctcc ttgaggaagg aattgctgct caggaaggtc agcgggatga cgccgatacc
     2101 ggtccagtat cctttggaag acttgaactg ggcaggaatc gccgacgctt cggagggtac
     2161 atacacctcg aacacccctt cgagaacacc ggcctcatag gtatcggccg gcccgcccca
     2221 gagagcgtcg acctgggggt tcgccttctc ggcgatcaat cgggcaaggg cttcaccgga
     2281 tgaaaggcgg acgaaattga cctggatccc cgtgtccttc gtgaactcct caaaaaccgt
     2341 ggttgcatac ttttcaggca tgatcgtgta cacgttcagc gtggtacttg ctttctcctg
     2401 gacaccggcg gcgaacaccg gggtcagaag caacccaatc atgcttaaca cgatcaaggc
     2461 tttcttcatg cttttcatag aaatcctcct taggtagttg gccgaaatac ttcggctcca
     2521 ccttcatcct tcgccaagga gggtgaaaat acaagttaca gctttgtaac aaatatctcc
     2581 taggacacct cttgagtgtc ggtaagcagg tatccgaacc cccacctgtt caccagaaac
     2641 cgcggtgtcg aggggttcgg ctcgattttt ttccggaggc gactgatatt caccttcacc
     2701 atttccggcc cctggatcca ctcctcggca ttccatacct gcttgagaag ctcttcggcg
     2761 gatacgggga catgagcatg gagcgccaga tattcgatga ttctgaactc ggtgggagtg
     2821 agggtgatct cctgcccgtc gatctccaat acccgtctgc ggatatcg
//
LOCUS       DAOPUZ010000138         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_36257_length_2861_cov_2.371703, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000138 DAOPUZ010000000
VERSION     DAOPUZ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36257_length_2861_cov_2.371703"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PKN79_03110"
     CDS             complement(<1..616)
                     /locus_tag="PKN79_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ94783.1"
                     /translation="MTKRPPILELEGIVKEFSTVRVLDQVNVSIYPGEIMGLVGENGA
                     GKSTLIKIICGIYEETSGTIRLDGKDIRIPDYITAKSLGIGLVPQEFNLINYLTVYEN
                     IFLGNERKKGVFLDRPKMREEAKAQLESLKMPIDVHRTVSDLSVAEKQMVEIAKAMVL
                     DARILIFDEPTTALTGVEVQHLFALMRKLKEQGVTMIFVSHKLGE"
     gene            complement(616..1608)
                     /locus_tag="PKN79_03115"
     CDS             complement(616..1608)
                     /locus_tag="PKN79_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ94784.1"
                     /translation="MARNVTIFTGQWADLPFEEVCKIISEMGYDGLEIACWGDHMDVK
                     KAATDPAYVKDRLATLKKYNLGCWALGNHLAGQCVGDAFGDPRLAGFAPKALAGDDEA
                     IRAWGIEQMKYTAQAAKNMGCYVVTGFMGSPIWKYFYSFPANTPELIEEGYQEILRLW
                     TPILDEFDKQGVKFALEVHPSEIAYDYYSTQKLFEVFGGRKTLGINFDPSHLQWQGVD
                     PALFFRDFADSIYHIHIKDSVVKLDGRSGILGSHLMFGDLRRGWNFVSPGHGDVNFDM
                     IIREANAAGYQGPLSVEWEDNGMDRLFGAREALEMVRRIDFAPSTIDFDGAMEN"
     gene            complement(1623..2762)
                     /locus_tag="PKN79_03120"
     CDS             complement(1623..2762)
                     /locus_tag="PKN79_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ94785.1"
                     /translation="MKHIAYGMVGGGPGAFIGDVHRKAIRMDGLAVLKAGSFSRDAAK
                     SKAHGKTLGVSEDRLYDDYREMARKEGSREDGIKFVVVVTPNSTHFEVCKAFLEQGIH
                     VVCDKPLTWKIEESEELQRLAQKKNLLFAVTYTYTGYPAVKQMRKMVRDGLLGKIRFV
                     NAEYPQDWLANPVDESSPIAPWRMDPEVSGISNCLGDIGSHIENLVSTVTGLKIKRVC
                     ARLDSLVEGRTLDDNATVLVEYEGGAKGMYWSSQVAFGYDNALRIRVFGEKGGLEWAQ
                     EDPDHFIFTPSDAPKQVFSRGRDPFAEEAQRYSRIPSGHPEGVYEAFANIYLPFVTAL
                     AKHLRGEKLEASDLDFPSVDQGLDGVRFINRCVQSSKQGALWVEM"
BASE COUNT          642 a          817 c          698 g          704 t
ORIGIN      
        1 cttcaccaag cttgtgactg acgaagatca tcgtgacccc ttgctccttg agcttgcgca
       61 tcaaagcgaa gaggtgttgc acttcaacgc cggtgagggc ggtcgtcggc tcgtcgaaga
      121 tgaggattcg agcatcaagc accatcgctt ttgcgatttc aaccatctgc ttttcggcaa
      181 ctgaaaggtc gctcaccgtt cgatggacgt cgatcggcat cttcagcgat tcaagctggg
      241 ctttcgcctc ttcccgcatc ttgggcctat cgaggaacac tcccttcttt cgctcattgc
      301 caaggaagat gttctcatat accgtgagat agttgatcag gttgaactct tggggaacca
      361 ggccgatccc caggcttttc gcggtgatgt agtcgggaat gcggatatct tttccgtcaa
      421 gacggatcgt tccgcttgtt tcctcataga ttccgcagat gatctttatc agggtggact
      481 tgccggctcc gttttcaccg acaagcccca tgatttcacc cggatagatc gacacattca
      541 cctgatcaag gacccgaacc gttgagaact ctttaacgat cccttccaac tccaaaattg
      601 gtggacgctt tgtcattaat tctccattgc tccatcaaaa tcaattgtgg aaggggcaaa
      661 atcgattcgt cggaccatct ccaacgcctc ccgggcgcca aagagccgat ccatcccgtt
      721 gtcttcccat tcaacggaaa gcgggccctg atatccggcg gcgtttgctt cccggatgat
      781 catatcgaag ttgacgtccc catgcccagg cgagacgaag ttccatcccc ggcgcagatc
      841 gccgaacatc agatgggatc cgagaatacc gcttcgtcca tccagcttca ccacggaatc
      901 cttgatatgg atgtggtaga tcgagtcggc aaaatctctg aagaaaagcg ccggatcgac
      961 cccctgccac tggaggtggc tcgggtcgaa gttgattccc aacgtttttc ttccgccgaa
     1021 cacctcgaac agcttttgcg tcgaatagta gtcgtaggcg atctccgagg ggtgaacctc
     1081 gagggcgaac ttgacgccct gcttgtcaaa ctcatcgaga atcggggtcc acagcctgag
     1141 aatctcctga tatccctcct cgatcagctc gggagtattt gccgggaagg aatagaagta
     1201 tttccagatc ggcgagccca tgaaaccggt taccacatag cagcccatgt ttttcgccgc
     1261 ctgagcggtg tacttcatct gctcgatacc ccaggcgcgg atcgcttcgt cgtctcccgc
     1321 caacgctttc ggtgcgaatc ctgcaagacg ggggtctccg aacgcatcac cgacgcactg
     1381 gccggcaagg tggttgccga gggcccagca gccaagattg tatttcttca gcgtcgccag
     1441 cctgtctttc acgtatgcgg gatcggtggc cgctttcttg acgtccatgt ggtcgcccca
     1501 acaggcgatc tcaagtccgt cgtaccccat ttcactgatg atcttacaca cttcctcaaa
     1561 gggaagatcg gcccattgac cggtaaatat cgttacgttc ctagccattg tatctctcct
     1621 ttttacattt caacccagag cgcgccctgc ttgctgcttt ggacgcatcg attgatgaag
     1681 cgaactccat caaggccttg atccactgat gggaaatcca gatcggaagc ttccagtttt
     1741 tctcctctca gatgttttgc caatgcggta acaaagggaa ggtaaatatt ggcaaatgct
     1801 tcatagaccc cttcagggtg accggaagga attcggctgt atcgttgagc ctcttcggca
     1861 aacgggtcgc ggccgcggct gaacacctgc tttggagcat cgcttggagt aaagatgaaa
     1921 tggtcgggat cctcctgagc ccactccaat ccgccctttt caccgaagac tcggatccga
     1981 agggcgttgt catagccgaa ggcgacctgg ctggaccaat acatcccttt tgccccacct
     2041 tcatactcca caagaaccgt agcgttatca tccagcgtcc gcccctcgac aagggaatca
     2101 aggcgcgcac agacccgttt gatcttcagg ccggtgacgg tggatacgag attttcgatg
     2161 tgactgccga tatcgccgag acaattggaa atgccggaga cttcgggatc catccgccag
     2221 ggggcgatcg gacttgactc atcgacggga ttggcgagcc aatcctgcgg gtactcggcg
     2281 tttacaaacc gaatctttcc caaaagaccg tcgcggacca ttttccgcat ctgcttcacc
     2341 gcaggatatc cggtatatgt gtatgtaacc gcaaagagaa gattcttctt ttgggccaaa
     2401 cgttgaagct cctcactctc ttcgattttc cacgtcaacg gcttgtcgca gactacatgg
     2461 atgccctgtt ccaaaaaggc tttacatact tcaaagtggg tgctgttcgg agtcacgacg
     2521 acgacgaact tgattccgtc ctcgcgtgag ccctccttcc tagccatctc gcggtaatcg
     2581 tcgtacagtc ggtcttcgct cactccaagg gtcttgccat gagcttttga cttggctgca
     2641 tcccgactga atgaacccgc ttttaaaacg gcgagtccat ccatcctgat cgctttgcgg
     2701 tggacatcac cgatgaacgc accgggacct ccccctacca ttccataggc aatatgtttc
     2761 ataacccctc catttgttat ctaaagcgta tcacactgaa aagagatttc cataagcaag
     2821 attgatgatt catatgtaat attgacatat ggagagcaaa a
//
LOCUS       DAOPUZ010000139         2851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_36413_length_2851_cov_3.227825, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000139 DAOPUZ010000000
VERSION     DAOPUZ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36413_length_2851_cov_3.227825"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKN79_03125"
     CDS             complement(<1..605)
                     /locus_tag="PKN79_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ94786.1"
                     /translation="MINTPTFADLGLSAQTIQAIENKGFIHPTGIQATCIPLLLKDRV
                     DVIGQAQTGTGKTAAFGLPILELVEERLNAVQALILAPTRELALQSAEEILSLKGGRN
                     LEIAAVYGGAAIVNQLHQLKKGVHVVVGTPGRILDHLRRGSLVLDQLKFLVLDEADEM
                     LDMGFLEEIEAIMARTPKEKRTLCFSATMPDPIRRLAERYM"
     gene            complement(670..1704)
                     /locus_tag="PKN79_03130"
     CDS             complement(670..1704)
                     /locus_tag="PKN79_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HNZ94787.1"
                     /translation="MQHEVTNNQPLLGKNGRIIEEGWARRPVWKYDRSMVKGTAFRIK
                     EWEYYAIVNQDEQWATAVTFSDLGYAGVFAIAYIDLKTGRVSQTDAIAPLTLGKLGIP
                     ATSTENHELAWANKHIRIALVKRGEERHIMYACPSLDLGDGIIGLDVDITLKQPSSLE
                     SMTIATSWKKNRTCFYHNEKVNCLGATGTIRRGTALQRLPLGSTFGVLDWGRGKWTYR
                     NTWYWASCSGTVEQVPFGLNLGYGFTDRSPASENVIIYDSVVHKLCEVTFEYGSYLEE
                     WTIRDDEGRLDLVFQPVADRSSHFNLLLIASEQHQIFGHFSGRCTLDNGNTIVVDKIA
                     GFAEEVANRW"
     gene            complement(1774..>2851)
                     /locus_tag="PKN79_03135"
     CDS             complement(1774..>2851)
                     /locus_tag="PKN79_03135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ94788.1"
                     /translation="KDGTFDVDTYQKATTERKSAIESSIRRNLPYRMVMTDVSTVLAS
                     EAEREYVGNMASKVRDFSYLLFDSSLYPDSEAIAYALEHKQLFENIEISIISLGSLED
                     AEEVASSLQSGLYTFEEAAREHSLDMYAQADGYIGTIPFFAIKPNFRYEDEAAALLGA
                     ASGSIVGPFETGGGWAIYRVSTAAEEADVADENTRNLVKTYIGSNEEELMDSYLSNLV
                     TEVSEYAKKTDLVEAALEYDLDLVEVMPTGYNVGKSSFLNNFSYTDPTGMLASYATAE
                     VQTELFTSAEHTLLEPMKTGGAYLLIEVGEDSTDEGMGSYTMMFYDYLAGSQSQENLA
                     QTLYASDLHKDNFLTTFLRVVLGQ"
BASE COUNT          601 a          756 c          795 g          699 t
ORIGIN      
        1 tccatatagc gttcggccag gcggcggatc ggatcgggca tcgtggcgct gaagcagagg
       61 gttcgcttct ctttcggtgt ccgggccatg atcgcttcga tctcctcgag aaaacccata
      121 tccagcatct catccgcttc gtcgaggacg aggaacttga gttggtccag cacgagcgag
      181 ccgcgtctca gatgatcgag aatccgcccg ggagttccca cgacgacatg aacgcctttc
      241 ttcaattggt gtaattgatt gacgatcgcc gcacctccgt agaccgcagc aatctcgagg
      301 ttcctcccgc ctttgagcga aagaatttct tcagcgcttt gaagggcaag ttcccgggtc
      361 ggggcaagaa tgagagcttg aaccgcattc agtcgctctt caacaagttc aaggatcgga
      421 aggccgaacg cagccgtttt tccggttccc gtctgcgctt ggccgatgac atccactcgg
      481 tctttcaaaa gaagagggat gcatgttgcc tgaatgccgg tagggtggat aaatcctttg
      541 ttttcaatcg cctgtattgt ttgagcggac aggccgaggt cagcgaacgt tggtgtgttg
      601 atcatgaagc tcccgtgtgt tttggcaccg ggaagagtgt ataggaagag gatgacactt
      661 gcaagacggc taccaccgat tggcgacctc ttcggcaaat ccggcgatct tgtccaccac
      721 gatggtgttt ccgttgtcca atgtgcatct gccgctgaaa tgaccgaaga tctggtgttg
      781 ctcgctggcg attaaaagca gattgaagtg tgaagagcgg tcggcgaccg gttggaagac
      841 gagatcgagc cgtccctcgt cgtccctgat cgtccactct tccagatacg agccatattc
      901 gaacgtcacc tcacaaagct tgtgcaccac tgaatcgtag atgatcacat tctcgctcgc
      961 cgggcttcga tcggtgaacc cgtagccgag attcagtccg aaggggactt gttcgacagt
     1021 gccgctgcag cttgcccaat accaggtgtt cctgtacgtc catttgcctc ttccccaatc
     1081 caatactccg aacgtcgagc ccagcggaag ccgttgcagg gcggttcccc gacgaatcgt
     1141 tcccgtcgcc ccgagacagt tcactttctc attgtgatag aagcaggtgc ggttcttctt
     1201 ccagcttgtg gcgatcgtca tggattcaag ggaggagggc tgcttcaggg tgatgtcgac
     1261 atcaagaccg atgatgccat cgcccaaatc cagagagggg cacgcataca tgatgtgccg
     1321 ctcctctccc cgtttgacga gggcgatgcg aatgtgcttg tttgcccatg cgagctcgtg
     1381 gttttccgtt gacgtagccg gtatgcccag cttcccaagt gtgagcgggg cgatcgcatc
     1441 ggtttgactg actcgaccgg ttttgaggtc gatgtaggcg atcgcaaaca ctccggcgta
     1501 tccgagatcg ctgaacgtta cggcagtagc ccattgttca tcctggttga cgattgcata
     1561 gtattcccac tctttgattc ggaaggcagt tcccttgacc atcgatcggt catacttcca
     1621 taccgggcga cgagcccagc cttcttcgat gatcctgccg ttttttccca aaaggggctg
     1681 attgttcgtt acttcgtgct gcatggctcc atcgtaccat atcaaaaagc gatgagccat
     1741 gaaaaaccgg cccgaaggcc ggccggtatc cgcttattga cccaaaacga ctcgcaggaa
     1801 cgtcgtcagg aagttgtcct tgtgaaggtc ggaagcatag agcgtctggg cgaggttttc
     1861 ctggctctga cttccggcaa ggtagtcata gaacatcatc gtgtaactgc ccattccctc
     1921 atcggttgaa tcttcaccga cttcaatcag gagatacgcc cctccggttt tcatcggctc
     1981 gaggagggtg tgttcggcgg aggtgaagag ctccgtttgt acttcggctg tcgcatagga
     2041 ggcaagcatc ccggtaggat cggtatagga gaagttgttc aggaaggagg actttccgac
     2101 gttgtatccg gttggcatga cctcgacaag atccagatcg tactcaagcg ccgcctctac
     2161 caagtcggtt ttttttgcat actcggaaac ctcggttacc aagttgctga gatagctgtc
     2221 catcaactct tcctcgttgc tgccgatata tgttttgacg aggttgcgtg tattctcatc
     2281 ggcaacgtcc gcctcttcgg ctgcagtgga tacgcggtag atcgcccatc cgccgccggt
     2341 ttcaaacgga ccgacgattg aaccggaagc ggcacccaat aaggcggccg cttcatcttc
     2401 gtagcggaag ttcggcttga tggcgaagaa gggaatcgtt ccgatgtatc catccgcttg
     2461 ggcgtacata tcgagactgt gctcgcgggc ggcttcctca aaagtataga gtccgctttg
     2521 caaggaagag gcaacctctt cagcatcttc caatgaaccg aggctgatga tcgaaatttc
     2581 aatattttca aagagctgct tatgctccag tgcgtaggcg atcgcttctg aatccggata
     2641 gagggaggaa tcgaagagga gataggagaa gtcccgaacc ttggaagcca tgtttccaac
     2701 atattcccgc tcggcctcgg aagcgaggac ggtgctgacg tccgtcatca ccattcggta
     2761 ggggaggttg cggcggatcg aggattcgat tgcgctcttc cgttcggtag tggctttctg
     2821 gtaagtatcg acatcgaacg ttccgtcttt g
//
LOCUS       DAOPUZ010000140         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_36523_length_2844_cov_2.866260, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000140 DAOPUZ010000000
VERSION     DAOPUZ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36523_length_2844_cov_2.866260"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PKN79_03140"
     CDS             <1..909
                     /locus_tag="PKN79_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94789.1"
                     /translation="IGETLNYSVPFILCGLSMGFANRVGLFNIGGEGQYIMGMTVAQI
                     IALLGPQIPVLHWLLALLGAMLIGAVWGGVVGILKAKYEVSEVVSTIMLNYVALYLSR
                     IICLQLPGATTYRTASYPETALITNVFFQKITKNSLLNNGIFFMILAVVAFWFIMEKT
                     SLGYGMRATGFNKEAARASGIPVVRSISISMAISGIFAGLAGGIIALGSFKYGRVIAG
                     MDNYGFDGIAVALVGNSTALGTTLAGMLFGLLKNAQALMQAKQIPKEITFIIQGLIVI
                     FIALRSALRIYKEYLDKRRLQKEVGL"
     gene            906..1814
                     /locus_tag="PKN79_03145"
     CDS             906..1814
                     /locus_tag="PKN79_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94790.1"
                     /translation="MMIWNMLPSVLMIVAPILITAIGGMISEKSGVVNIALEGLMMIG
                     AMSAAAAHVLMEGSGMNHTLSLVLAMVIGTVIGGLFSLLHAVAAINLNADQTISGTGI
                     NLLASGVTIFASQILFNADRTKEFRMGMQTDALGFYPTAYIALVVVLLSWFVLYKLPF
                     GMHLRACGEHPGAAESVGINVKRIRYIAVLASGMLAGLAGGCVVLTQTIQYTSNTING
                     RGFIALAAVSFGRWRPAGVAGAALLFGTAQAFAIIANNIPALRVLPTEVFNILPYVVT
                     LVALVLFSGKNYAPAAAGRPYEKGAS"
     gene            1814..2515
                     /locus_tag="PKN79_03150"
     CDS             1814..2515
                     /locus_tag="PKN79_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNZ94791.1"
                     /translation="MSVHIAAKKVASVVLAPGDPLRAKLVAERFLEKSRLITDIRNIL
                     GYTGIYKGVEISVIATGMGGPSIGIYSYELFNEYDVETIIRIGTCGGFKKEILPGDRI
                     LALTASTDSAWAHQYNLKGTLSPACNPDLLLTAMLLDPSAHVGMVFSSDLFSSYNALG
                     EESWKAWAAMGALAQDMETYALYATAAHANKRALSMLTMTDCLATGAALIDDERQSAL
                     YPMIELALEVAYREL"
     gene            2518..>2844
                     /locus_tag="PKN79_03155"
     CDS             2518..>2844
                     /locus_tag="PKN79_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ94792.1"
                     /translation="MNFLDIMRHRFACKLYDVDKPLSQEEIDEILEYGRLTPTSFGLE
                     MWHFHAVVSKEKLKELYTVCYEQEGVRTSGMTVAILVKAPKHLDPDGSFVYERGIRFP
                     SDIDWFI"
BASE COUNT          557 a          861 c          831 g          595 t
ORIGIN      
        1 atcggcgaga cgttgaacta ttcggttccc ttcattctgt gcggtctttc gatggggttt
       61 gccaaccgcg tcggcctgtt taacatcgga ggggaaggcc aatacatcat ggggatgacg
      121 gtcgcccaga ttatcgccct gctgggaccg cagattcccg tgctgcactg gctgctcgcc
      181 ctcctggggg cgatgctcat cggggcggtc tggggcggag tcgtcggaat cctgaaggca
      241 aagtacgagg tcagcgaggt cgtctccacg atcatgctca actacgtcgc cctgtatctc
      301 tcgcggatca tctgtcttca gctgcccgga gcgacgacct atcggacggc gagctatccc
      361 gagaccgccc tgatcaccaa cgtcttcttc cagaagatca cgaagaactc gctcttgaac
      421 aacgggatct tcttcatgat tctcgccgtc gtcgccttct ggttcatcat ggagaaaacc
      481 agcctgggct acgggatgcg ggcgaccggc ttcaacaagg aagccgcccg ggcaagcggg
      541 atccccgtcg tccgttcgat ctccatctcg atggcgatca gcggaatctt cgccggcctc
      601 gccggcggga tcatcgctct cggctccttc aagtatggaa gggtgatcgc cggcatggac
      661 aactacgggt tcgacgggat cgccgtcgcc ctggtcggga actccaccgc gctgggaacc
      721 acccttgccg gcatgctctt cggactcttg aagaacgccc aagctctgat gcaggccaag
      781 cagatcccca aggagatcac cttcatcatc caaggcttga tcgtcatctt catcgccctg
      841 cgctcggcgc tgaggatcta taaggaatat ctcgataagc gccggcttca gaaggaggtg
      901 ggactatgat gatctggaac atgcttccct ccgtcctgat gatcgtggcc ccgattctca
      961 tcacggcgat cggggggatg atcagcgaga agagcggggt cgtgaacatc gccttggaag
     1021 gattgatgat gatcggggcg atgagcgccg ccgcagccca cgtcctgatg gaagggtcgg
     1081 ggatgaacca caccctttcc cttgttctgg cgatggtgat cggaacggtg atcggcgggc
     1141 tcttctccct ccttcacgcc gttgccgcga tcaacctcaa tgcggatcag acgatcagcg
     1201 gtacgggaat caacctgctg gcaagcgggg tgacgatctt cgcttcgcag attctcttca
     1261 acgcggaccg gaccaaggag ttccggatgg ggatgcagac cgacgccctc gggttctatc
     1321 cgaccgccta catcgccttg gtcgtggtgc tcctttcgtg gttcgttctc tataagcttc
     1381 ccttcgggat gcacctcagg gcatgcggcg aacacccggg agccgctgaa agcgtcggta
     1441 tcaacgtgaa gcggatccgc tacatcgccg tcctggccag cgggatgctc gccggtcttg
     1501 ccggcggatg cgtcgtgctg acccagacga tccagtatac ctccaatacg atcaacgggc
     1561 ggggattcat cgcccttgca gcggtctcct tcgggcggtg gcgccctgcc ggggtggccg
     1621 gtgccgccct cctcttcgga accgctcagg cgttcgcgat catcgcgaac aacatacccg
     1681 ccctgagggt gctgcccacc gaggtcttca acatcctgcc ctacgtggta accctcgtcg
     1741 ccctcgttct cttcagcggg aagaactatg ctccggccgc tgcgggaaga ccctatgaaa
     1801 agggggccag ctgatgagcg ttcacatcgc ggcgaaaaaa gtcgcttccg tcgtgctggc
     1861 cccgggagat ccgcttcggg cgaagctggt cgccgagcgc tttctcgaaa aaagcagact
     1921 gattaccgat atccgaaaca tcctcgggta caccggcatc tataaagggg tggagatctc
     1981 cgtcatcgcg acggggatgg gcggtccttc gatcggcatc tacagctacg agctgttcaa
     2041 cgagtatgac gtcgagacga tcattcgaat cggcacctgc ggcggtttca aaaaggagat
     2101 tcttcccggg gatcggatcc tcgcccttac cgcgtcgacg gatagcgcct gggcccatca
     2161 gtacaacctg aaaggcaccc tcagtcccgc ctgcaatccg gatctgctcc tcacggctat
     2221 gcttcttgat ccaagcgcgc atgtggggat ggtcttctcc agcgatctct tctcctccta
     2281 caacgctctc ggcgaagaga gctggaaagc ctgggcggcg atgggcgcac ttgctcagga
     2341 tatggagaca tacgccctct atgcgaccgc cgctcatgcg aacaagcggg ctctttccat
     2401 gctcacgatg accgactgcc tcgcaacggg agccgcgctt atcgatgacg agcggcaaag
     2461 cgccctctat ccgatgatcg agctcgccct cgaggtagcc tatagggaac tgtgaagatg
     2521 aactttctcg acatcatgcg ccatcgcttc gcctgcaaac tctacgatgt ggataaaccc
     2581 ctgagtcaag aggagatcga tgaaattctg gagtatggcc gactgacccc gacctccttc
     2641 ggccttgaga tgtggcattt ccatgcggtc gtttcaaagg agaagctcaa ggagctctat
     2701 acggtctgct atgaacagga gggggttcgg accagcggaa tgacggtggc gattctggtc
     2761 aaagccccga aacacctcga tcccgacgga tcgttcgtgt atgaacgggg gatccgcttc
     2821 ccgagcgaca tcgactggtt catc
//
LOCUS       DAOPUZ010000141         2836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_36680_length_2836_cov_3.149586, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000141 DAOPUZ010000000
VERSION     DAOPUZ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36680_length_2836_cov_3.149586"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1209)
                     /gene="uxuA"
                     /locus_tag="PKN79_03160"
     CDS             complement(127..1209)
                     /gene="uxuA"
                     /locus_tag="PKN79_03160"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HNZ94793.1"
                     /translation="MLHMTLRWFGPNHDTVPLNYIRQIPGVEGVITTLYDIPAGEVWP
                     SERIRELKAEVEAAGLKILGIESVNIHDAIKIGNEDRERYIDNYIETLRNLGKEGITT
                     VCYNFMPVFDWTRTDLAKERPDGSTVFAYDEEIVRSIDPQTLFDHTTASSEGFLMPGW
                     EPERLAKIKDLFEAYKEVDEKTLFDNLVYFLKRIRPVAEEYDIKMAIHPDDPVWPVFG
                     LPRIITGLDKVKKLMKAVDAPFNGVTFCTGSFGTNPKNDLPEMIRALEGRIHFAHIRN
                     LEHVEEGVFEESAHLSRDGSFDLYEIVKALHDIGFDGPVRPDHGRMIWDEVAMPGYGL
                     YDRALGAMYILGLEEAIVKNQEMKNA"
     gene            1360..2022
                     /gene="rpe"
                     /locus_tag="PKN79_03165"
     CDS             1360..2022
                     /gene="rpe"
                     /locus_tag="PKN79_03165"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNZ94794.1"
                     /translation="MNTPSLIIAPSMLASDFSRGAAEVASIKNHGADWVHLDVMDGAF
                     VPNISFGPVVIESLRKHSDLPFDTHLMIERPERYIADFAAAGSDYLTIHYEGNVHLDR
                     TLNLIKEHRVKAGISIVPSTPVDALKEILHLIDLVLVMSVNPGFGGQKMIPSTLKKIT
                     RLKDLREECGYTYLISVDGGVNLDTVSSIVEAGVDVAVTGSAFFGAEDRTRFVQRMKG
                     LP"
     gene            2019..2456
                     /gene="dtd"
                     /locus_tag="PKN79_03170"
     CDS             2019..2456
                     /gene="dtd"
                     /locus_tag="PKN79_03170"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNZ94795.1"
                     /translation="MRAVIQRVYDASVTVDGEVVGSIERGLLVYLGVGEGDTEEQLKW
                     ICEKIPKIRCFRDEHGKMNLDLKQIDGQILLVSQFTLLANLRKGNRPSYDRAADPKIA
                     EALYDKAFSLFEEQGVPVSGGVFGAHMHVRYTNDGPVTFTLDA"
     gene            2507..>2836
                     /locus_tag="PKN79_03175"
     CDS             2507..>2836
                     /locus_tag="PKN79_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HNZ94796.1"
                     /translation="MAKSKDSSFPLDLKQKQDALEAARAQIDKQFGKGSLMKLGDADE
                     TLNIESIPSGSLLLDEALGIGGYPRGRIVEIYGPESSGKTTIALHAIAEAQKAGGIAA
                     FIDAEHAM"
BASE COUNT          660 a          736 c          754 g          686 t
ORIGIN      
        1 cggttcaata gagcttcccg tatcacaaat cagacaattc cggaagaaat tgaaacgatg
       61 tattcaagat aggaaatggt ctctctttga ttcctgttac gtctttttca aacaggggcc
      121 ggttggttaa gcgttcttca tttcttggtt tttgacaatt gcttcttcaa gcccgaggat
      181 gtacatggca ccgagagccc ggtcatacaa gccataaccg ggcatcgcaa cctcgtccca
      241 gatcatccga ccatgatcgg ggcggaccgg accatcgaaa ccgatgtcat gaagcgcttt
      301 gacgatttca tacaggtcga atgatccatc ccgggaaagg tgggccgatt cttcaaagac
      361 tccttcttca acgtgttcga ggttccggat gtgggcaaag tggattcggc cttcaagagc
      421 ccgaatcatt tcgggcaagt cgtttttcgg gttggtcccg aagctgccgg tgcagaacgt
      481 cactccgtta aacggcgcgt ccactgcctt catcagcttt ttgaccttgt ccaacccggt
      541 aatgattcgc ggcaatccga aaaccggcca gacgggatcg tcgggatgaa tcgccatctt
      601 gatgtcatac tcctccgcta cgggccggat acgcttaagg aagtacacga ggttgtcaaa
      661 gagagtcttt tcatcgacct ctttgtacgc ttcaaaaaga tcttttattt tcgcaaggcg
      721 ttcgggttcc caacccggca tcaaaaagcc ctcgcttgat gcggtagtat gatcgaacag
      781 ggtctgggga tcgatcgatc tgacgatctc ctcgtcgtag gcaaaaacgg tcgatccgtc
      841 ggggcgctct ttggccagat cggtgcgggt ccagtcaaaa accggcatga agttatagca
      901 caccgtagtg attccctctt taccgaggtt cctcaacgtc tcgatatagt tgtcaatata
      961 ccgttcccgg tcttcgtttc cgatcttgat cgcatcgtgg atgttcacgc tttcgattcc
     1021 gagaatcttc aagccggcgg cttcaacctc ggccttgagc tcgcgaatgc gttcgcttgg
     1081 ccaaacttcc ccggcgggga tgtcgtacaa ggtggtgatg accccctcta caccgggaat
     1141 ctgccgaata tagttcaacg gaacggtgtc atggttgggg ccaaaccatc tcagtgtcat
     1201 atggagcatg ggtatgtttc cttatcgttt aatgagcaca ggctagcata tttacatatc
     1261 ggcaacaatc ttcaaccggc ggaacacacc cgtggcaacc tcgattcttt cctcagccaa
     1321 tcgttgcata tcggcctgct tcttggtata actgagggta tgaatacacc ctcattgatt
     1381 attgcaccga gcatgcttgc cagcgacttt tccagaggag cggccgaggt ggcttcgatt
     1441 aagaaccatg gggcggattg ggttcacctt gacgtgatgg acggggcttt tgttccgaat
     1501 atcagctttg gaccggttgt catcgagagc ctcagaaagc acagcgacct tcccttcgac
     1561 acgcatctga tgattgagcg accggagcgt tatatcgctg atttcgccgc cgccggcagt
     1621 gattacctca cgattcatta tgaaggaaac gtccatctgg atcgcaccct gaatctgatc
     1681 aaagaacatc gggtcaaagc gggaatcagc atcgttccct ccacaccggt cgatgcattg
     1741 aaggagatcc ttcatctcat cgatctggtt ctggtcatga gcgtgaaccc cggctttggg
     1801 ggacagaaga tgatcccgtc caccttgaaa aaaataaccc gattgaaaga tttgagagag
     1861 gagtgcggct acacctatct catcagtgtc gatgggggag ttaatctgga caccgtttct
     1921 tccattgttg aagcgggtgt cgacgtcgcc gttaccggca gcgccttttt tggcgccgaa
     1981 gaccgtaccc ggttcgttca acggatgaag ggtcttccgt gagagcggtc attcagcgcg
     2041 tctatgacgc gagtgtcact gtcgatggcg aggttgtcgg ctccatcgaa cggggtctgc
     2101 tcgtatatct gggagtgggc gagggggaca cggaagaaca gctgaagtgg atctgcgaaa
     2161 aaattcccaa gatccgctgt tttcgggatg aacacggcaa gatgaacctc gacctgaaac
     2221 agatcgacgg ccaaattctt ctcgtcagcc agttcactct gctggcaaac ctccgcaaag
     2281 gcaaccgccc ctcctacgac cgggcggcgg acccgaagat cgccgaagcg ttgtacgaca
     2341 aagcgttttc cctgtttgaa gaacagggcg ttcccgtctc cggtggagtc tttggcgcac
     2401 atatgcatgt cagatataca aatgatggtc cggttacctt tacccttgat gcataagttg
     2461 atgcaatatg gtactatacc gcccataagg aaccagagga gttaccatgg caaaaagtaa
     2521 agacagttcg tttcccttgg atctgaagca aaagcaggat gcccttgagg cggcccgagc
     2581 ccaaatagac aagcagttcg gaaaggggtc gctgatgaag ctcggtgatg ccgatgaaac
     2641 gttgaatatc gaatcgatcc cctccggttc gctcctgctt gatgaagccc tcggcatcgg
     2701 cgggtatccg cggggcagga tcgttgagat ttatggtccc gagtccagcg gaaaaaccac
     2761 gattgccctt catgcgatcg ccgaagcgca gaaagcggga ggaatcgccg cgttcatcga
     2821 tgccgagcat gcgatg
//
LOCUS       DAOPUZ010000142         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_36703_length_2835_cov_2.720504, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000142 DAOPUZ010000000
VERSION     DAOPUZ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36703_length_2835_cov_2.720504"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PKN79_03180"
     CDS             complement(<1..652)
                     /locus_tag="PKN79_03180"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254742.1"
                     /note="involved in coenzyme B(12)-dependent 1,
                     2-propanediol degradation; important for the synthesis of
                     propionyl coenzyme A during growth on 1,2-propanediol;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HNZ94797.1"
                     /translation="MVILTLNCGSSSAKYQVYDWEEKEVLAVGVVERIGLEYSTIEHK
                     AVGKEEYTAEFSSPTHKEAIELIIRMLLDKEYGVIGDLKEIGAVGHRVLHGGEAFKKS
                     ALITDEVIAQLKSYIHLGPLHMPANIMGIEGAVKAMPDVPQAIILDTAWHQTMPEDAF
                     MYAVPYEWYSKYNVRRYGFHGTSHLYCAKRAAAILGKKNKDTNVIICHIGNGASVNA"
     gene            781..1803
                     /locus_tag="PKN79_03185"
     CDS             781..1803
                     /locus_tag="PKN79_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94798.1"
                     /translation="MRRFSFLCLLLFASAALWAEANLLLCFEPLQKTFKASVETAGLS
                     LDLSYRFTSEMPAPSVALGPFRIKTTRITVRNTTLHPLFANELFETRLEHPQWGRLSL
                     FRADGRHIGLGFEWGAFTGIFIRLPILEPKNVFLPHRKHQGLTVFLRYSRGPVTVYCI
                     GSAHTPLSLQGSVVLHAGAFTMRSVLDSSAFPYRLTLGLEGGKASLSHEFSVGREPIY
                     SGSEQVLRVSQKSNVTISIGSFVIGLDYLHTLQTKEDRMVRTDLRIRATIKHASNRIY
                     LVWREQEGWSFELKGGNGSIVLSKEGVAYEFAAHLEQGEVRISLTSKGVFSIAYTGRF
                     TIGQRR"
     gene            complement(1781..2686)
                     /gene="murB"
                     /locus_tag="PKN79_03190"
     CDS             complement(1781..2686)
                     /gene="murB"
                     /locus_tag="PKN79_03190"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNZ94799.1"
                     /translation="MPINVSNRVEKINLEQDVNLAKYSTIKSGGTADLFCYPTDIQEL
                     RAALAFARERSIPVTVLGGMSNTLISDEGIEGLTIGTARLNKVKKRGNLLSVLAGAQL
                     EETVNVAIEEGLGGLEHFYGIPGTIGGALWGNAGAHDHQISETVLFADFLDLHGNLFR
                     KQIIQSEFSYRMSPYKGRSDLILYEVHLALNPISDTHTLKTIALGYKQRRIEQQQFRH
                     PSLGSIFKNPPGHKAGALLEGLGLKGTRIGGAQIAPHHANMIINVDGSATSTDIRTLI
                     DMAKEKVFQKYGITLEEEITFVGRW"
BASE COUNT          632 a          745 c          715 g          743 t
ORIGIN      
        1 ccgcattgac cgatgcgccg tttccgatgt ggcagatgat gacgttggtg tctttgttct
       61 tctttccgag aatcgccgcc gctcgtttgg cgcagtagag gtggctggtt ccgtggaacc
      121 cgtagcgacg gacgttgtac ttcgagtacc attcgtacgg cactgcgtac atgaatgcat
      181 cctcgggcat cgtctggtgc caggcggtat cgagaatgat cgcctgagga acatccggca
      241 tcgcttttac cgcgccttcg atgcccatga tgttggcagg catatgcaac ggacccaaat
      301 ggatatagct cttgagctgg gcaatgacct cgtcggtgat caatgcggat ttcttgaagg
      361 cttcgccgcc gtgcagtacc cggtgtccca cggccccgat ctctttcaga tccccgatta
      421 cgccatactc tttgtccaag agcatgcgga tgatcagctc gatcgcttcc ttgtgggtag
      481 gggaagaaaa ctcggcggta tactcctctt taccgaccgc cttgtgttca atcgttgaat
      541 actcaaggcc aatacgttcg accacgccga cagcaagcac ttctttttct tcccaatcat
      601 agacttggta ctttgcagaa gaactgccac aattcagggt taaaattacc atgaagcact
      661 cctcattttg gatataacat tacaaagctc ggttggcacg ggtgccacaa agtattaaac
      721 cattattgag acgaagaatc aacataaagt agagatggaa actcttttcc ttaagaagaa
      781 atgagacgtt tttcctttct ctgtcttctt ctcttcgcat ccgcagcgct ttgggcggag
      841 gcgaaccttc ttctgtgctt tgagccgttg caaaaaacct tcaaggcatc ggtcgagact
      901 gcgggattgt ccctcgatct ttcctaccgc ttcacctcgg agatgcctgc accttccgtt
      961 gctctcggcc cgttccgaat caaaacgact cgaatcaccg taaggaatac gacacttcat
     1021 cctcttttcg ccaatgaatt gttcgaaact cgccttgaac acccccaatg gggaaggctt
     1081 tccctctttc gggcggatgg gaggcatatc ggtcttggat ttgaatgggg agcctttacg
     1141 ggaatattca tccgtcttcc gattcttgag ccgaagaacg tgtttcttcc tcatcgaaaa
     1201 caccaaggat tgacggtgtt tcttcgctat tcccggggac cggtcacggt gtattgcata
     1261 ggcagcgccc acacaccgct ttcgctccaa gggtcggtcg tgcttcatgc aggagctttc
     1321 acaatgcgga gcgttcttga ttcaagtgcg tttccctatc gtcttaccct cggattggaa
     1381 ggtggaaaag cttcactttc acatgagttc agcgttggta gggaaccgat ctacagcgga
     1441 agcgagcaag tgcttcgagt ctcccaaaaa agcaacgtaa caatatccat cggctccttt
     1501 gtcatcggac ttgattatct tcacactctg caaacaaaag aagacaggat ggttcgaacg
     1561 gatcttcgca tccgggcgac gatcaagcat gcatcgaatc gaatatacct tgtctggcgt
     1621 gaacaggaag ggtggtcttt cgagctgaag ggcgggaatg gatcgattgt tctttccaaa
     1681 gaaggagttg cgtacgagtt tgccgctcac ttggagcagg gtgaagttcg gatttcgctc
     1741 acatccaaag gggtattttc catcgcctac acgggccggt tcaccatcgg ccaacgaagg
     1801 tgatctcttc ttcaagggtg attccatact tctgaaagac cttctctttg gccatatcga
     1861 tcagggtgcg gatatcggtg gaggtggcgc tgccatcaac gttgatgatc atattggcgt
     1921 ggtgcggagc gatctgagcc cctccgatcc gtgttccctt gaggcctaac ccctccagca
     1981 acgcccctgc cttatgaccg ggcgggttct taaagatcga gccgagggaa gggtggcgaa
     2041 actgctgttg ttcgatccgt cgttgtttat atcccaaggc aatcgttttg agcgtgtggg
     2101 tatcgctgat cgggttcaag gccagatgca cttcatagag gatgagatcg ctccgccctt
     2161 tatacgggct catccgatag gagaattcgc tttggatgat ctgctttcga aagaggttgc
     2221 cgtgaagatc gagaaaatcg gcaaacagca ccgtttcact gatctggtga tcgtgggctc
     2281 cggcatttcc ccaaagcgcc ccaccgatcg taccgggaat cccatagaag tgttcaaggc
     2341 ctcccaaccc ctcttcgatc gcaacattga ccgtttcttc cagctgcgcc cctgcaagaa
     2401 cggagaggag gttcccccgc tttttcacct tgttcaatcg agcggttccg atggtaagac
     2461 cttcgatccc ctcgtcgctg atcagggtgt tgctcatgcc tccgagaacc gtaacgggga
     2521 tcgatctttc acgagcgaat gcaagggcag ctctaagttc ttgtatatca gttggatagc
     2581 aaaacagatc cgccgttccg ccactcttaa ttgttgaata tttggctagg ttgacatcct
     2641 gttcaaggtt gattttttca accctattgc ttacattgat aggcatgaag acatcttagc
     2701 cctatttatc gggtcaagca atccaaagga gcttgtcatg gcgcttcgcc tgtataatac
     2761 aatgagtcgc tcgatcgaag agttcatccc gatcaccgcc aacaggtcgc tcccgttcgc
     2821 cgccgtgaag aaggt
//
LOCUS       DAOPUZ010000143         2776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_37774_length_2776_cov_3.184491, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000143 DAOPUZ010000000
VERSION     DAOPUZ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37774_length_2776_cov_3.184491"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2701)
                     /locus_tag="PKN79_03195"
     CDS             complement(<1..2701)
                     /locus_tag="PKN79_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94800.1"
                     /translation="MAKRQMQGGIDLDQAERELEAITQFAGKGLKEARTVVAAKAKLN
                     ESKKRIGETKELGEEIQRVKTSLANRPELQARGKRLEEIRSYLELKHAYERQKAEVEK
                     HDGRLASLRHTNLDVLSNLEKAAANARDSVDAGQKELLELTRKLDELAVPEHLLDYKE
                     AVDRFEDELDEAEDLHVKLQNGTENLIRKKGELESWNGQLGWLASEPPKEEALQKMVS
                     SLGKLARTDEALRSAVQANAKLLKRYPKPTEDQKEYRSELIERKHKLLELATGKKKLT
                     KGEQAVYWILLSIIALLTGAGIYFGMNGFTREPIILGALSVMLLSVLGRKVVWDLKRE
                     HTVDIKEFIRYFEAQPSTTYDQILQICFTELAELDEKMKVNDEVQAEFEEAKKRYEEH
                     IAAYEEIYEALGIPHDSALEGARFFNAGEHLRLWSNLLGSVRSQETVVAELQASFDQR
                     IEALEALCQSTFGNDPVREAKRFLDRLKQAQTTKETLERYQRSLDESKEQLALAEAER
                     RAWYERYEIESREVAEALFSRMEEYKSLADDLELQKRNLAKVDETIVEEARHIDRQTF
                     DIEFEALQQTLEKLEDLHEHFITIQEQYRLASRASEHENALYEYESSLEALDAHRRDA
                     LKKRLVYSIIADLKEETEREHQPAVIQRSSAWLGRITHNRYSLNAGKEDFRAFDHAED
                     RSLALEELSSGTRIQLLFALRMGYLESLEEGSNAALPIFFDEIMANSDDERSLAIAEA
                     IGLIAEERQVFYATAQTDEVLKLKNQAETPPRLIDLEQLRSKEAVERRPFTAPKIVPR
                     KEIPFEEEYHQYAKTLMIAQAGLFDPVETLSSWYLCLDSKELQELLTRNLDKAGTARW
                     SSPLLSRRFLLLKEARHLAQAGREKTLTAADLSDPELTLNRSTN"
BASE COUNT          564 a          800 c          693 g          719 t
ORIGIN      
        1 aattggtcga tcgattcagg gtgagctccg gatcgctcag gtcagcggct gtcagtgttt
       61 tctcccgtcc cgcctgagcc agatgccggg cctccttcaa gaggagaaac cgtcttgaaa
      121 gcaacggtga gctccatctc gcagttcccg ctttgtcaag gttacgcgtg aggagctctt
      181 gcaactcttt gctgtcaaga caaagatacc acgaagaaag ggtctcgacg ggatcaaaga
      241 ggccggcttg agcgatcatc aacgtcttgg cgtattggtg gtattcctct tcaaagggga
      301 tctcttttct gggaacgatc tttggtgcgg tgaacggccg cctttcgacc gcctccttcg
      361 agcgcagctg ctcaagatcg atcagacgag gaggggtttc cgcttgattt ttgagcttaa
      421 gcacctcatc ggtctgggct gtcgcataga agacttgccg ttcttcggcg atcaagccga
      481 tcgcttcggc gatcgccagc gatcgttcat catcgctgtt cgccatgatt tcatcaaaga
      541 agatcggaag agccgcattg ctcccctcct ccagactttc caagtacccc attctcagag
      601 cgaaaagcaa ctggatgcgc gttccgctgg agagctcttc aagagcaagg gaccgatctt
      661 cggcgtggtc gaacgctctg aagtcctcct tcccggcatt cagtgaatat cggttgtgag
      721 tgatccgccc aagccatgcc gacgaacgct gaatgaccgc aggctggtgt tcccgctcag
      781 tctcttcctt cagatcggcg atgatcgagt agacaagccg tttcttaagg gcgtccctac
      841 gatgggcatc caacgcttcc agcgagcttt cgtactcata cagggcgttc tcatgctcgg
      901 aagccctgct cgccaaccga tactgctcct gaatcgtaat gaaatgctcg tggagatctt
      961 caagcttctc caatgtttgc tgaagcgctt caaactcgat atcgaacgtt tggcgatcaa
     1021 tgtgccgtgc ttcctccaca attgtttcat caaccttggc aaggttcctt ttttgcagtt
     1081 caaggtcgtc agcaagactc ttgtactcct ccatccggct aaagagcgct tcggcgacct
     1141 cccggctctc gatctcataa cgctcatacc atgctcgccg ttcagcctcg gcaagagcaa
     1201 gctgctcctt ggattcatca agcgatcgtt gataacgttc aagcgtctct ttcgtcgttt
     1261 gggcttgctt cagccgatcc agaaagcgct tcgcttcacg aaccgggtca ttcccaaacg
     1321 tggattggca aagcgcttca agggcctcga tccgctgatc gaatgaagct tgaagttcag
     1381 cgaccacggt ctcttgggaa cgaacggaac cgagcaggtt tgaccagagt cgaagatgct
     1441 cccccgcatt gaagaaccgg gcgccctcca acgcggaatc atgcggtatt cccagcgctt
     1501 cgtagatctc ttcataggcg gcgatgtgtt cctcatatcg cttcttcgct tcttcgaact
     1561 ccgcctgtac ttcatcattg accttcatct tttcatcgag ttcggcaagc tcggtgaaac
     1621 agatctgcag aatctggtcg tacgtagtcg atggttgggc ttcgaagtat cggataaact
     1681 ccttgatatc aacggtatgt tcgcgcttaa gatcccagac cacctttcgg ccgagaacgg
     1741 aaagcagcat gaccgacaac gcaccaagga tgatcggctc ccgggtgaac ccgttcatcc
     1801 cgaaatagat tccggcaccg gtcaagagag cgatgatgga aagcagaatc caatataccg
     1861 cctgttcacc tttggtgagc tttttcttcc ccgtagccag ttccagaagt ttatgctttc
     1921 gctcgatcag ctcgctccgg tattccttct gatcctcggt gggcttcgga taccgcttca
     1981 gcagcttggc attggcttgg acagcgctcc gcaatgcttc atcggtccgg gcgagcttac
     2041 ccaaggagga gaccatcttc tgaagcgctt cctccttcgg cggttcggaa gcgagccatc
     2101 cgagttgtcc gttccagctc tccagctctc ccttcttccg aatgaggttc tccgtaccgt
     2161 tttggagctt gacatggagg tcttccgcct catcaagttc atcctcaaac cggtccaccg
     2221 cctccttgta atcaagaaga tgctcaggaa ccgccagctc atcgagtttc cgagtgagtt
     2281 ccaaaagctc cttttgtccg gcatccacgg aatcccgagc attggctgca gctttttcaa
     2341 ggttggaaag cacatcaagg ttcgtgtgtc tcaacgaggc gagtcgtccg tcatgctttt
     2401 cgacttccgc tttctgccgc tcataggcat gtttcagttc aaggtaggaa cggatctctt
     2461 ccagcctctt tcctcttgcc tgaagctccg gtcggttggc caagcttgtc ttcacccgtt
     2521 gaatctcttc accgagctcc ttggtttccc cgattcgttt cttggattca ttcagcttcg
     2581 ctttggcggc gacgacagtt cgcgcttctt tgagcccttt tccggcgaac tgggtgatcg
     2641 cttcgagctc tctttccgcc tgatcaaggt cgattccccc ttgcatctga cgcttcgcca
     2701 tttcaaagaa gactgtatga tgttgcagcg ccggggcgac aagctcgtga agtgcgaacc
     2761 aataggattc acgaaa
//
LOCUS       DAOPUZ010000144         2759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_38135_length_2759_cov_3.070636, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000144 DAOPUZ010000000
VERSION     DAOPUZ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38135_length_2759_cov_3.070636"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKN79_03200"
     CDS             <1..420
                     /locus_tag="PKN79_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ94801.1"
                     /translation="SFDNGDVAYNTAKELFKKYPNLKGIMGTSSYDAPGVARAIEELG
                     LVGKAFTSGTGMPLDNAELLKSGVVKSLTLWDPALAGKAMIALAVKVLNGEQITGPVN
                     LNVEGYEALQFKTGSKTVLEGEGWIVINAANVYDFGF"
     gene            520..2004
                     /locus_tag="PKN79_03205"
     CDS             520..2004
                     /locus_tag="PKN79_03205"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNZ94802.1"
                     /translation="MAETLLRAQSIYKSFVGVQALKDVSFSIKRGEIHCLAGENGSGK
                     STLIKIISGVYTPDSGHIEFDGHQYSKITPIEAINLGIQVIYQDFSIFPNLTVMENLA
                     INTELAANRKFVNWKRIRRVAEEALAKINVRIDLDAYVGSLSVAEKQIIAISRALMFN
                     TKLIIMDEPTTALTKKEVRNLFAIILQLKERGIATLFVSHKLNEVFEISENFTILRSG
                     ELVATGSTKELDDKKFSYYMTGRTFESRRFTPETVTKEPIFEADRVGIPGSFKDVSFA
                     LHGGEIIGITGLLDSGRTELALSMFGMKPIREGEFRVDGTKVELTTPREAIRHGIGYV
                     PEDRLSEGLFLPQPIGDNIVITELDSLTKRFGVIDTDRRDEEIRTWVKNLAIATPNPD
                     NACQTLSGGNQQRVVLAKWLANNPRILVLNGPTVGVDIGSKHDIHTILQELANEGMGI
                     IIITDDLPEVVENCSKILVMKSGKIVAKLAAEEADEQKILAYML"
     gene            2018..>2759
                     /locus_tag="PKN79_03210"
     CDS             2018..>2759
                     /locus_tag="PKN79_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94803.1"
                     /translation="MKGGTPTLLKKLTRRNEFYVFLVLLVLCLLIEVRSGQFFSPNNL
                     VDIASAMIVPGLFAIGAFLVIVSGGVDVSFPALASLTMYATTKMLLDANYQGGAALPI
                     AIALVIGAVLGAFNGLFIGYFKLPAMIVTLGSSSVFKGLMQGALNSKQLTVIPPGMRR
                     WGTTALFIARNPVSGLTSRMYVTFIAFIAVLALVFFLLRFTMFGRGIYAIGGSEVSAH
                     RAGFNVIRTKVTMYVLVGMIASLAGIIRA"
BASE COUNT          636 a          753 c          780 g          590 t
ORIGIN      
        1 tcgttcgaca acggagacgt tgcctacaac acggccaaag agcttttcaa gaagtatccc
       61 aacctgaagg gaatcatggg aacatcctcc tatgacgccc cgggtgtcgc tcgtgcgatc
      121 gaagaacttg gtcttgtcgg aaaagcattc acctccggca ccggaatgcc gctggacaac
      181 gccgagctgc tgaagagcgg cgtcgtcaag tccctgacgc tctgggatcc cgcactcgcc
      241 ggcaaggcga tgatcgccct ggcggtgaag gtcctcaacg gcgagcagat caccggaccg
      301 gtgaacctca atgtcgaagg atatgaagcc ctccagttca agacgggaag caagaccgtc
      361 ctcgaaggcg aaggctggat cgtaatcaac gcagcgaacg tctacgactt cggattctag
      421 tgttgttact atccgattac tgtcctgtca aagactcttt ggcaggacag tatcgtgtta
      481 tactgtagct tacagaacta ccaccacgag gtgatatcga tggcagagac gttattacga
      541 gcccagtcaa tctataagtc gtttgtcggg gttcaggcat tgaaggacgt gtcgttctcg
      601 atcaagcgcg gggagatcca ctgcctcgcc ggtgaaaacg gaagcggaaa atcgaccctg
      661 atcaagatca tcagcggcgt gtacaccccc gattccggac acatcgagtt tgatggacat
      721 cagtactcca aaatcactcc gatcgaggcg atcaatcttg gaattcaggt catctaccag
      781 gacttctcca tcttccccaa tctgaccgtg atggaaaacc tcgctataaa caccgaactg
      841 gccgcaaacc gcaagttcgt gaattggaag agaattcggc gggtagccga agaagctctt
      901 gcaaaaatca acgtccggat tgatctggat gcatatgtcg gatcattgtc ggtcgcggaa
      961 aagcagatca tcgcgatcag ccgggccctc atgttcaaca cgaagctgat catcatggac
     1021 gagccgacga ccgccctcac caaaaaagag gtgaggaacc tgtttgccat catccttcag
     1081 ctgaaagagc gcggcatcgc cacgctgttt gtcagtcata agctgaatga agtttttgaa
     1141 atctccgaga acttcaccat ccttcgcagc ggcgagctgg ttgcgaccgg ctcgacgaag
     1201 gagctggatg acaagaagtt ctcctactat atgaccggac ggacgtttga gtcccggcgg
     1261 ttcactcccg aaacggtaac caaggaaccg atttttgaag cggaccgggt cggcattccc
     1321 gggtcattca aggatgtttc gtttgctctt catgggggtg agatcatcgg aatcaccgga
     1381 ctgctggact ccggacggac cgagctggct ctttcgatgt tcggaatgaa gccgatcagg
     1441 gagggggagt tccgggtgga tggaaccaag gtcgagctta ccacccccag agaggcgatt
     1501 cgccatggga tcggatacgt tcccgaggac cgcctcagtg aggggctctt tcttccccag
     1561 ccgatcgggg acaacatcgt aatcaccgaa ctcgattcat tgacgaaaag gttcggtgtc
     1621 atcgataccg atcgccgtga tgaagagatc cgcacgtggg ttaagaacct tgcgatcgct
     1681 actcccaatc ccgacaacgc gtgccagacc ctctcgggag gaaaccagca gcgggtggtg
     1741 ttggcgaaat ggctggcgaa caatccccgg attcttgtcc tcaacggccc gacggtcggc
     1801 gtggatatcg gctccaaaca tgatatccat acgattctcc aagagctggc gaatgagggg
     1861 atggggatca tcatcatcac cgacgacttg ccggaagtcg tcgaaaactg ctcaaaaatc
     1921 ctggtgatga agagcgggaa gatcgtggcc aagctcgccg ccgaagaagc ggacgaacag
     1981 aagatcctgg cgtatatgtt gtagggaggg agacggaatg aaaggcggta caccgacatt
     2041 gctgaaaaaa ctgactcgac gaaacgagtt ctatgtattt ctcgttctcc ttgtgctctg
     2101 tttgctgatt gaagtccgat cgggacagtt cttctcgccc aacaacctcg tcgacatcgc
     2161 ttcggcgatg atcgttcccg ggctgttcgc gatcggagcg ttcctggtca tcgtttccgg
     2221 aggggtcgac gtttcctttc cggctcttgc gagcctgacg atgtacgcga ccacgaagat
     2281 gctgcttgac gccaactacc aggggggagc ggcgcttccg atcgcgatcg ccttggtgat
     2341 cggagccgtt ctgggagcgt tcaacgggtt gttcatcggg tacttcaagc ttccggcgat
     2401 gatcgtgacc ctcgggtctt ccagcgtgtt caaaggcctg atgcagggag ccctcaactc
     2461 gaaacagctg acggtgattc ccccggggat gagacgatgg ggaaccaccg ccctcttcat
     2521 cgcccgcaac cccgtctccg gcctgaccag caggatgtac gtcacgttta tcgcattcat
     2581 cgcggtgctc gccctcgtct tcttcctgct tcggtttacg atgttcggaa gggggatcta
     2641 tgcgatcggg ggctcggagg tcagcgccca ccgggcgggc ttcaatgtca tccggaccaa
     2701 ggtgaccatg tacgttctgg tcggcatgat cgccagtctt gccggaatca tccgagcca
//
LOCUS       DAOPUZ010000145         2758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_38158_length_2758_cov_2.664447, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000145 DAOPUZ010000000
VERSION     DAOPUZ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38158_length_2758_cov_2.664447"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PKN79_03215"
     CDS             complement(<1..1320)
                     /locus_tag="PKN79_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ94804.1"
                     /translation="MKKTQIENFNQDIHVVDYRTPFETFRKQADSLIAIEGRETESLN
                     GKYHFSPDPYESCLRARWFEEKTVSEDGRMLPFDADFDVWPRIPVPSCWNMVREELYY
                     YENMGLYFRTFRYKPVKNHERAFIHFEGSAYRTYVFLNGTCVGIHDGASTPFSVEVTD
                     HLQPENRLIIAVDAARSKDRVPMDNTDWFNYGGIYRDILFIRTPETFIRNFQTQLKPG
                     KTNRVFAAVELDKEVDAVATYRIRELEIEHEIAIRDGKGQIEFDADLKLWSPEHPVRY
                     EVEITCGEDRVKDLIGFRDIQVDGHKILLNGKRIFLKGICIHEDHITLGKSTTEEVIR
                     ASIADVKAMNGNFIRLAHYPHSRLFAKIADEVGILLWEEIPVYWSINFTNPETYADAE
                     NQLSELILRDRNRASVIIWSVGNENPDSDERLAFMSSLAKKTRELDPT"
     gene            complement(1334..2164)
                     /locus_tag="PKN79_03220"
     CDS             complement(1334..2164)
                     /locus_tag="PKN79_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ94805.1"
                     /translation="MTSYRRKQRIEKIARYVVLAIVVFITLFPLVIVLSSSFKNESEL
                     FDFPFKFIPAKPITDNFTRLKENFPLYIWNSVKLTFVIVIVQLFTAATGAYVFTKLHW
                     KGRDFIFGLYIASMMIPMQAVMIPQFLIIRSINLYDTHMSLVLLGAFTAFGTFLIKQF
                     FMSIPDTYCEAARIDGAKEWTIFSTLMLPMSKAVLVTQIIFSFRYFWNDFFTPLIYIT
                     TPQLKTLPLGMTDFVREQYVYWGPQMAAALISIVPVLLIFLFGQKYFVQGVASSGIKG
                     "
     gene            complement(2151..>2758)
                     /locus_tag="PKN79_03225"
     CDS             complement(2151..>2758)
                     /locus_tag="PKN79_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ94806.1"
                     /translation="FCGCSFFRGALYLPNILSSVAVGLAFMFIFEPTSGFMNQFLISV
                     GLPTSKWLAGSKSALSVIITIAIWQNFGYYMVLFIGGLQNISMTLYEAAGIDGANKWK
                     QFVHVTLPGLSPMLFYGVTIAVIRGFQVFDYIYIMTGGQQGGGPAGSTNVLAFDIYLN
                     AFTYFRFGYASAESVVLMIIILAMTFLQSHGQKKWVSYDIV"
BASE COUNT          605 a          751 c          781 g          621 t
ORIGIN      
        1 ggtcgggtcg agctccctcg tcttcttcgc cagcgaggac atgaaggcaa gccgctcgtc
       61 ggaatccggg ttctcattcc ccaccgacca gatgatgacg cttgcgcggt tccgatccct
      121 gaggatcagc tccgaaagct ggttctccgc atcggcatag gtttccggat tggtgaaatt
      181 gatcgaccag tacacgggaa tctcctccca gaggaggatc cccacctcgt cggcgatctt
      241 tgcaaacagc cgtgagtgcg gatagtgggc aaggcggatg aagtttccgt tcatcgcctt
      301 gacatcggcg atcgaagcgc ggatcacctc ctcggtggtc gatttgccca aggtgatgtg
      361 gtcttcgtgg atgcagatcc ctttgaggaa gatccgcttg ccgttgagga ggatcttgtg
      421 tccatcgact tgaatgtccc ggaacccgat gaggtctttc acccggtctt ccccacaggt
      481 gatctccacc tcgtatctga cgggatgctc cggcgaccag agcttgaggt cggcatcgaa
      541 ttcgatctgc cccttcccgt cacggatcgc tatctcatgc tcgatctcca gttcccggat
      601 gcgataggtg gcaacggcat cgacctcctt gtccagctcg accgcagcga acacccggtt
      661 cgtcttcccc ggcttcaact gggtctggaa gttcctgatg aaggtttcgg gggttcgaat
      721 aaagaggata tcccgataga tgccgccgta gttgaaccag tcggtgttgt ccatgggaac
      781 ccgatccttg ctccgagcgg catcgacggc gatgatgagc cggttttccg gctgcagatg
      841 atccgtcacc tcgacggaga acggggtgga cgccccgtcg tgtatcccga cgcatgttcc
      901 attgaggaac acgtaggtcc ggtaggcgct tccttcgaaa tggatgaacg cccgttcatg
      961 gttcttcacc ggcttatacc tgaaagttct gaagtagagc cccatgttct catagtagta
     1021 gagctcctcc cgaaccatgt tccagcagga gggaacgggg attctcggcc agacatcgaa
     1081 gtcggcatcg aagggaagca tgcggccgtc ttcgctgacg gtcttctctt cgaaccagcg
     1141 ggcccgcagg cagctttcgt acggatccgg agagaaatgg tatttgccgt tgagcgattc
     1201 ggtctctcgc ccctcgatgg caatcaggct gtccgcctgt ttccggaagg tctcgaacgg
     1261 ggtgcgatag tccaccacgt ggatatcctg attgaagttt tcaatttggg tctttttcat
     1321 ctctgtttcc ttgtcatcct ttgattcccg acgatgcgac accttggacg aagtatttct
     1381 gtccgaagag gaagatgagt aacacgggga cgatggagat cagggccgcc gccatctgcg
     1441 gcccccagta cacatactgc tcccgcacga aatccgtcat ccccagcggc aaggtcttga
     1501 gctgcggcgt ggtgatgtag atgagcggcg tgaagaagtc gttccagaag taccggaacg
     1561 agaagatgat ctgggtgacc agtaccgctt tgctcatcgg aagcatcaat gtcgagaaga
     1621 tcgtccattc cttggctccg tcgattctgg ccgcttcgca gtacgtgtcg ggaatcgaca
     1681 tgaagaactg cttgatgagg aaggtgccga acgcggtgaa cgctccaagc agcaccaagg
     1741 acatatgcgt atcgtagagg ttgatgctcc tgatgatcaa aaactgcgga atcatcaccg
     1801 cctgcatcgg aatcatcatc gatgcgatgt acagcccgaa gatgaaatcc ctccccttcc
     1861 aatggagctt cgtgaacacg tacgcccccg tcgccgcggt gaacagctgc acgatgacga
     1921 tgacgaacgt cagcttgacc gagttccaga tatagagggg aaagttctcc ttcaaccggg
     1981 tgaagttgtc cgtgatcggt ttcgcgggga tgaacttgaa cgggaagtcg aagagctcgc
     2041 tttcgttctt gaagcttgac gagaggacga tcacgagcgg gaacagagtg atgaacacca
     2101 cgatcgccaa caccacatat cttgcaatct tttcgattcg ctgctttctt ctatacgatg
     2161 tcatagctca cccacttttt ctgtccatgg ctctgcagga acgtcatcgc cagaatgatg
     2221 atcatcaaca cgaccgactc ggccgatgca tagccgaacc tgaagtacgt gaacgcgttg
     2281 aggtagatgt cgaacgcaag gacgttggtg gatcccgccg gaccgcctcc ctgctgaccc
     2341 ccggtcatga tgtatatgta gtcgaatacc tgaaagcccc tgatgaccgc gatcgtgacg
     2401 ccgtagaaga gcatcggcga gagcccggga agcgtgacat ggacgaactg cttccatttg
     2461 ttcgcaccgt cgatccctgc ggcttcgtac agcgtcatgc tgatgttctg aaggcctccg
     2521 atgaagagaa ccatgtagta tccgaagttt tgccagattg cgatggtgat gatgaccgaa
     2581 agcgccgact tgctgccggc aagccatttt gacgtcggca gtcccaccga tatgagaaat
     2641 tggttcatga acccgctggt gggttcgaaa ataaacatga atgcgaggcc gacggcgacg
     2701 gaggagagga tgttcggcag atacaaagct cctcggaaaa acgagcagcc gcagaagt
//
LOCUS       DAOPUZ010000146         2752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_38267_length_2752_cov_3.010382, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000146 DAOPUZ010000000
VERSION     DAOPUZ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38267_length_2752_cov_3.010382"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /gene="polA"
                     /locus_tag="PKN79_03230"
     CDS             <1..1867
                     /gene="polA"
                     /locus_tag="PKN79_03230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNZ94807.1"
                     /translation="GMRTIVGELERLSGEKALKPLEKPKKDRRTYRGVTDLGELKSLF
                     ESAVKSGGIIAFDLETDTVVELDATIIGFSFAWEAYTAYYVPLVHQGKTLVDEGGVKS
                     LLRTYLEEGRLKVVGQNIKFDYKVLKVRWNITIRHIHADTMIAAWLLDAASTLNMDFL
                     ALRYLGVETLRYTDIVPKDKTLAEIDYEKALFYAAEDADITLRLWQSFEELLKEADLL
                     DLLRTLELPILRIIAHMELEGIFLDEGVIDPLEEEFAGRIDRIKEQIFELAGKEFNLN
                     SPKQLQEILFVEQGIPTGAKTRTGYSTASDVLEPLSGQYPLVALVLEYRELNKLASTY
                     VEALPKQINRRTGRIHPTFQQTGTETGRLSCRDPNLQNIPVRTEEGRRIRSAFVPRTG
                     CVFLSADYSQIELVVLAHMADDPGLKEAFLGGMDVHRFTASLIFDTPMTEVTSEMRAI
                     AKTINFGVMYGMGTHALAQDLKIAHQEAKRFIEQYFERYPAVRDFVEETKTRARKEEV
                     VHTLLGRRRPVGEINSRNAVERARAERIAVNTIIQGSAADIMKQAMIRVDRMMRERRL
                     LSALILQIHDELVFEVPLDEVEEMKALVKEAMEGAVTLSIPLNVSISVGSNWGEL"
     gene            1864..2469
                     /gene="coaE"
                     /locus_tag="PKN79_03235"
     CDS             1864..2469
                     /gene="coaE"
                     /locus_tag="PKN79_03235"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269858.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNZ94808.1"
                     /translation="MKPVVIGITGKACAGKNQYAKAFEELGARLIDVDRLGHQALEAN
                     KDRIIAEFGDSVADRGEIDRKALGAIVFSDPSKLKALEGISHPWMVARIERILAEETA
                     EVIVLNAALLNRMGLDRLCSHILFITAPRCFRFRRCKARDGLSWRQFLKREASQRDIS
                     YQLLDPKAIHHRLWNWGSMEIIHRQVQRCCGRIGVKRCSRN"
     gene            2483..>2752
                     /locus_tag="PKN79_03240"
     CDS             2483..>2752
                     /locus_tag="PKN79_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94809.1"
                     /translation="MKKSIAIMLLVATSVVAIAVGIGIWYTRPSAPLAIIEEAKARQR
                     QPLLEVTPPQRAEVVPLPPDYQPLIDELLPLLQDELTQSKALSLEQ"
BASE COUNT          661 a          711 c          805 g          575 t
ORIGIN      
        1 cggcatgcga acgattgttg gggaactgga acgcctcagc ggtgaaaaag ccttaaagcc
       61 gcttgaaaag ccgaagaaag atcgacgaac gtatcggggg gtaacggatc ttggcgagct
      121 gaagagcctc tttgagagcg ctgtcaaaag cgggggaatc atcgccttcg atcttgaaac
      181 cgacacggtc gtcgaactgg atgcgacgat catcggcttc tcgttcgctt gggaagcgta
      241 tacggcgtat tatgttcccc ttgttcatca aggcaaaacg ctcgttgacg aaggtggtgt
      301 gaaaagcctt cttcgaacgt accttgaaga gggaaggttg aaggtcgtcg gacagaacat
      361 caagttcgat tacaaggtcc tcaaggtacg gtggaatatt acgatcaggc acatccatgc
      421 agatacgatg atcgccgctt ggctgctgga tgcggcctcg acgctgaata tggatttttt
      481 ggcgctccgc tatctgggcg ttgaaactct tcgctatacc gacatcgtac ccaaagacaa
      541 gacccttgcg gagatcgatt atgagaaggc gctcttctac gcagccgagg atgccgacat
      601 caccctccgg ctgtggcaga gctttgaaga gcttctaaaa gaggctgatc tccttgatct
      661 cctccgcacc cttgagcttc cgattctgag gatcatcgcc cacatggagc tggaggggat
      721 cttccttgac gaaggggtca tcgatcccct tgaagaggaa ttcgccgggc ggatcgaccg
      781 gatcaaagaa caaatcttcg agctcgccgg aaaagaattc aatctgaact ctccgaagca
      841 gctgcaggag atcctcttcg tcgaacaggg cattccgacc ggtgcgaaaa cccggaccgg
      901 atactcgacc gccagtgatg tcctcgagcc gctgagcggt caatatcccc ttgtggcgct
      961 ggtattggag taccgtgagc tgaacaaact cgcctcgacc tatgtcgagg cgttgcccaa
     1021 gcagatcaac cggagaaccg gacggattca tccaacgttt caacagacgg gtacggagac
     1081 cggccgcctc agctgccgcg accccaacct gcagaacatt cccgttcgaa ccgaggaggg
     1141 gaggcggatc cgatcggcgt tcgttcctcg aaccggttgc gtcttcctca gcgcggacta
     1201 ttcgcaaatc gaactcgtcg tccttgccca tatggcggat gatccaggac tgaaggaggc
     1261 gttcttaggg ggaatggacg ttcaccgctt caccgcgagc ctgatcttcg atacaccgat
     1321 gacggaggta acaagcgaga tgcgggcgat cgccaagacg atcaacttcg gggtaatgta
     1381 tgggatggga acccacgcgc tcgcccagga tctgaagatc gcccaccagg aggcgaagcg
     1441 gttcatcgag cagtattttg aacgctaccc ggcggtcagg gacttcgtcg aagagacgaa
     1501 gacccgggcc cggaaggagg aagtcgtcca caccctgttg gggcgccgtc gaccggtcgg
     1561 ggagatcaac agccgaaacg ccgtggagcg ggcccgggcg gagcggatcg cggtcaacac
     1621 gatcattcag ggcagcgccg ccgatatcat gaaacaagcg atgatccggg tcgaccggat
     1681 gatgagggag cgcaggctct taagtgcgct gattctccag atccacgacg aactggtctt
     1741 cgaagtcccg cttgatgagg tcgaagagat gaaagccctg gtcaaggaag cgatggaagg
     1801 ggcggtaacg ttgagcatac ccttgaacgt ctcgatcagt gtcggatcga actggggtga
     1861 gctgtgaagc cggtcgtcat agggatcacc ggcaaagcgt gcgccggaaa gaatcaatac
     1921 gccaaggcat ttgaagagct gggcgcccgt ttgattgacg tcgatcgcct cgggcatcag
     1981 gctctcgagg ccaataagga tcgaatcatt gccgagttcg gcgactccgt cgcagatcgg
     2041 ggagagatcg atcggaaagc gctcggagcg atcgtttttt ccgatccttc aaagctcaaa
     2101 gcgctggaag ggatcagtca tccgtggatg gttgctcgga ttgaacggat tctcgctgaa
     2161 gaaacggcgg aagtcatcgt tttgaatgca gcgttgctga accgaatggg cctcgatcgg
     2221 ttgtgttccc atattttgtt catcactgct ccgcggtgct tccgctttcg gcggtgtaaa
     2281 gcccgcgacg gtctttcatg gagacaattc ctgaagcggg aggccagtca acgcgacatc
     2341 agctatcaat tactcgaccc aaaagcaatc catcaccgac tctggaattg gggaagtatg
     2401 gagataattc atcgacaagt acaaaggtgt tgtggtagaa ttggggttaa gcgttgtagc
     2461 aggaactaaa tgaggattag agatgaagaa gtcaatagca attatgttac tggtagcaac
     2521 ctccgttgtg gcaatcgccg tgggcatagg aatctggtat actcgtccct ccgctcctct
     2581 tgccatcatt gaggaagcaa aagctcggca aaggcagcca ctcttggaag taactcctcc
     2641 tcagcgcgcc gaagtagttc cactgcctcc tgactatcaa ccgttaattg acgaattgct
     2701 tccgcttctg caggatgaat tgactcagtc gaaagccctc tcgcttgagc ag
//
LOCUS       DAOPUZ010000147         2724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_38835_length_2724_cov_2.420382, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000147 DAOPUZ010000000
VERSION     DAOPUZ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38835_length_2724_cov_2.420382"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1093
                     /gene="galE"
                     /locus_tag="PKN79_03245"
     CDS             107..1093
                     /gene="galE"
                     /locus_tag="PKN79_03245"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNZ94810.1"
                     /translation="MKILLVGGAGYIGSHVALQFLERGDQVGIFDNLSSGLKSNVDQR
                     ADFFEGDILNYEQIAAVLEEGWDAVIHLAAFKAAGESMIAPEKYSHNNISGSLNLIAA
                     SSKAKVKNFILSSTAAVYGEPEYLPVDEHHPTRPTNYYGYTKLAIEENLEWYSQLRDL
                     SYVSLRYFNAAGYDNEGRMLGLESNPANLIPVVMEVAMGIRPNLLIFGSDWDTPDKTA
                     IRDYVHVTDLATAHVLAVDHLMEGKGNLIVNLGSENGISVQQIVDTAREITGHPIPAE
                     YVERRKGDPAKLIASSKEARKTLGWKAVHSSVENIIASTWRVYLANQKRLES"
     gene            complement(1131..1203)
                     /locus_tag="PKN79_03250"
     tRNA            complement(1131..1203)
                     /locus_tag="PKN79_03250"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1168..1170),aa:Asn,seq:gtt)
     gene            complement(1313..>2724)
                     /locus_tag="PKN79_03255"
     CDS             complement(1313..>2724)
                     /locus_tag="PKN79_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNZ94811.1"
                     /translation="DVHARITPSDGGMGYSKLATMLEVGRSITDNILLLDAGDVSHGT
                     NLANLFEGETVGVLLDMLGYDAVAPGNHDFNYGYKRLVEAAEFAEEYSALRVLSANVV
                     DEDGYLVFQPYQVYDYNGFTVGVVGLTTPDTKTKSHPKNTEGLTFLNKEIFDLGQELV
                     DIARSYVDYIIVLGHIGLDPDGASGLTSEMIVRSINGIDLFVDGHSHTTLEKGLKVND
                     TYIVSAGDYLRNVGVVEINVRGGKVVGTTPMLIPAKDVLNPQESDLARAYGITAVPNN
                     AEVDEYVTYMQGQLNDVLNKVIAKIPMDLDGERANVRTRPTNLSKIIAAAMTEQSGAD
                     FSITNGGGIRASLKAGPVTVGDVVNVLPFTNILTVVEATGADVYAALEHGYRMLPDPN
                     GGFAQTDLSVVYNRFAEPGKRIIRVLLDGKPIDRNKTYRVATNDFLAAGGDGYTMFGR
                     ILTEGDLLSDVLIEYLSNQ"
BASE COUNT          638 a          757 c          716 g          613 t
ORIGIN      
        1 accctacttg cggttggtcg ttgagactgc cactcaagca ttgcttgatg aaaaactggc
       61 cgatctgagc acaatcattg cccgcttttc ataaggagat gaagcgatga agatattact
      121 agtcggaggg gccgggtaca tcggtagcca tgtcgctctc caattcctcg aacgaggaga
      181 ccaagtcgga atttttgaca acctttcaag cggcttgaaa agcaacgttg atcagcgtgc
      241 cgacttcttt gaaggggata tcttaaatta tgagcaaatc gccgctgtcc tcgaagaggg
      301 atgggatgcg gtgatccacc ttgcagcatt caaagccgca ggagagtcga tgatagcgcc
      361 ggaaaaatat tcccacaaca acatctcggg atccttgaac ctcatcgccg ccagctcgaa
      421 agccaaggtg aagaatttca tcctctcctc caccgccgcc gtctacggcg agccggagta
      481 tctgcctgtg gatgagcatc atccgacccg tccgacaaac tactatggat acaccaagct
      541 cgccattgaa gagaacctgg agtggtattc acagcttcgg gatctttcct acgtctccct
      601 gcgctacttc aatgcggctg ggtatgacaa tgagggtcga atgctcggtc tggaatcaaa
      661 cccggcgaac ctgattcccg tggtcatgga agtggcgatg ggaatcagac ccaatctctt
      721 aatcttcggc agcgactggg acaccccgga caagaccgca atccgtgatt acgtgcacgt
      781 caccgatctg gcgaccgccc acgtcttggc ggtcgatcat ttgatggaag gcaagggaaa
      841 cctcatcgtg aacctcggct ccgaaaacgg catcagtgtc caacagatcg ttgacaccgc
      901 ccgcgagatc accggtcacc cgattcccgc cgaatatgtg gagcgaagga agggcgatcc
      961 ggcaaagctc atcgcctcaa gcaaggaagc aaggaaaacc ctcggttgga aagccgtaca
     1021 ctcaagcgtt gaaaacatca tcgcttcaac gtggcgggtg tatttggcga atcagaaacg
     1081 actggagagc taaaaacgag aagagacaac cccgaaggat tgtctcatcg ctcgcccaga
     1141 gggatttgaa cccccgacag ggtggttaac agccacctgc tctaccgact gagctatagg
     1201 cgaatgtctg ttgaacgatt ggtacattac cctattggta ccatcgtgtc aagccggttt
     1261 cacgaaagag attattccaa aaggggcagc ccgggggctg cccctattca tgttattgat
     1321 tggaaagata ctcgatcagg acatcgctta ggaggtcgcc ctctgtgagg atccgaccga
     1381 acatcgtgta tccgtctccg ccggcagcca gaaagtcatt ggtcgccacc cggtaggtct
     1441 tgttcctgtc gatcggcttg ccgtcaagca ggactctgat gatccgcttg ccgggttcgg
     1501 cgaagcggtt gtacacgacg ctcagatccg tctgggcaaa tccgccgttt ggatccggca
     1561 gcattcggta cccatgctca agcgccgcat acacatcagc acccgtcgct tcgaccaccg
     1621 tgaggatgtt cgtaaacggc agcacgttga ccacatctcc gaccgttacc ggaccggcct
     1681 tcagggaagc acgaattccg ccaccgttgg tgatcgagaa atcggctccg ctctgttcgg
     1741 tcatcgcagc ggcgatgatc ttcgaaagat tcgtcgggcg ggttcgaaca ttggctcgct
     1801 cgccatccaa atccatcgga atctttgcaa tcaccttgtt gagcacatcg ttgagctggc
     1861 cttgcatgta ggtcacatac tcatcgactt cagcattgtt gggaaccgct gtgattccat
     1921 aggcccgagc aagatcgctc tcttgaggat tgagcacatc ctttgcggga atcagcatcg
     1981 gtgtcgttcc gaccactttg ccgcctcgga cgttgatctc caccactcct acgttcctca
     2041 gataatcacc tgcggagacg atgtaggtgt cgttgacctt caaacctttt tccagcgtcg
     2101 tgtgggagtg tccgtcaaca aacagatcga ttccgttgat gctgcgaaca atcatctcgc
     2161 tggtcaggcc gcttgcccca tccgggtcaa gaccgatatg tccaagaacg atgatgtagt
     2221 ccacatagct gcgggcgata tcaaccagct cctggccaag atcaaagatc tctttattga
     2281 ggaatgtgag tccctcggtg ttcttcggat ggctcttcgt ctttgtatcg ggggtcgtta
     2341 gaccgacgac accgacggtg aagccgttat agtcgtacac ctgatacggt tggaacacaa
     2401 gatacccgtc ctcatcaacc acgttggctg aaaggactct gagagcggaa tactcttcgg
     2461 cgaactcggc agcttcaacg aggcgcttgt acccatagtt gaagtcgtgg ttgccgggtg
     2521 ctaccgcgtc atagccgagc atatccagaa ggacgcctac cgtctctcct tcgaaaagat
     2581 tcgccaagtt tgttccgtgc gaaacatctc cggcatccag aagaaggatg ttatccgtga
     2641 tggagcgccc tacttccagc atcgtcgcaa gtttggaata gcccattccg ccgtcgctgg
     2701 gagttatgcg cgcgtgcacg tcgt
//
LOCUS       DAOPUZ010000148         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_39049_length_2713_cov_3.188111, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000148 DAOPUZ010000000
VERSION     DAOPUZ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39049_length_2713_cov_3.188111"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1159
                     /locus_tag="PKN79_03260"
     CDS             140..1159
                     /locus_tag="PKN79_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94812.1"
                     /translation="MTTPTMLKKAQSIYRSDRHVWRLIIMITLWMIFMAITRFRKFYT
                     LINFQTMAAQFPEFGLMSLGIMLAMITGGIDLSVVGTANLTSILMAFLLINLTTATGG
                     LPAYAIPLVFILAILLGAAFGLFNGLLISRFNIPPILATMGSGELFTGICMAMTNGNA
                     ISKFSRTYARTINNRLLDMIPVQLIIFTVMAVIIWFLLTKTAYGSKLYLLGTSPTVAR
                     FSGLKTRQLLMQTYMISGICAALGGMIMLANYNSARADYGTVYTLQCVLIVVLGGVSP
                     VGGRGKISGVVLAIILLRLLETGLNRFPKISSYYISLIWGGVLILVMVLNYFTENAKV
                     KTASI"
     gene            1168..1929
                     /locus_tag="PKN79_03265"
     CDS             1168..1929
                     /locus_tag="PKN79_03265"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HNZ94813.1"
                     /translation="MKKIRVLLAGESWVTFSTHHKGFDRFAAGMYDNGQRYLEAALKS
                     DPSIEYVHMPGHEVSERFPFTMEELKEWDVIIISDIGSNSFLLSNKVFLEGKKEANRL
                     QLLKEWTSGGGALVMCGGYLSFSGFQASAKFFRTPIEEVLPVDIYTFDDRVETPEGTE
                     ITIVDPKHPIVKDIKGPWPNFLGYQEAPLKSDAHLIAQSQYGHPLIAVRKYGKGTSMV
                     WMSDIGPHWCPLEFTQWEGYATFWRNAVHYLANDL"
     gene            complement(1916..>2713)
                     /locus_tag="PKN79_03270"
     CDS             complement(1916..>2713)
                     /locus_tag="PKN79_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNZ94814.1"
                     /translation="IIFRVGGDEFVLLLPQTDAEHAETILNRINNALKKEKVRNLLIS
                     VAFGSAQHNTEEGFETTFKTAEDRMYRNKLKENVTYKRQMIVSILDQLYEKEESIEEH
                     NKLVSSYAKDLAMQTQLSSEEVELIEKAALFHDLGKIAINKNILRLKTSQLTQVQTLE
                     LRRHPEIGYNILRSLDEYAPLAEAVLYHHERFDGNGYPRGLKGEEIPLYARIIAVANA
                     WANLTDPNSTSEQTPKSVAVASLKGMKGTVLDPDLVDCFLKKAFTSR"
BASE COUNT          582 a          722 c          774 g          635 t
ORIGIN      
        1 tcctgttggg tattcccacc tcgtggcgaa gcgttttcac cggtgtcctc atcatcgtcg
       61 gcacgggagt cagtgcctat cagacgatcg tgatgaatcg ctccaagcgg gtgcagaaag
      121 cggagaaggg ggtagcggca tgaccacacc gactatgttg aaaaaggctc aaagcatcta
      181 tcgaagcgat cggcacgtat ggcggctcat catcatgatc accctgtgga tgatcttcat
      241 ggcgatcacc cgcttcagaa aattctatac gttgatcaac ttccagacga tggccgccca
      301 gttccccgag ttcggactga tgagcctggg gatcatgctg gcgatgatca cggggggaat
      361 cgacctctcc gtcgtcggaa ccgccaacct gacctcgatc ctgatggcgt tcctgttgat
      421 caacctcacg acggcaaccg gagggctgcc ggcgtatgcg attccgcttg tgttcatttt
      481 ggcgattctt ctgggtgcgg cgttcggact gttcaacgga ctcttgatct cacgattcaa
      541 tattccgccg attctggcaa cgatgggaag cggcgagctc ttcaccggga tctgcatggc
      601 gatgaccaac gggaatgcga tcagcaagtt ttcccgcacc tacgccagaa cgatcaacaa
      661 caggctgctg gacatgatcc ccgttcagct gattatcttc accgtgatgg cggtgatcat
      721 ctggttcctc ctgaccaaga ccgcctacgg ttcgaagctg tatcttctgg gaacgagtcc
      781 gaccgtcgcc aggttcagtg gattgaaaac cagacagctg ctgatgcaga cctatatgat
      841 ctcgggcatc tgcgccgctc tgggtggaat gatcatgctc gccaactaca actccgcccg
      901 ggcggactat ggaacggtct acaccctcca gtgcgtgctc atcgtcgtcc ttggaggagt
      961 gagtcccgtg ggaggacggg gaaagatcag cggagtcgtt ctggcgatca tcctgcttcg
     1021 gctcctggag acggggttga accggttccc gaagatcagc agctactaca tctccctgat
     1081 ctggggcggc gtgctgattc tggtgatggt gctcaactat ttcaccgaga acgccaaagt
     1141 gaagacggca agcatatagg aggaactatg aagaagattc gagtactgtt ggccggagag
     1201 tcgtgggtca ccttttcaac ccatcacaaa ggattcgacc gctttgccgc aggcatgtac
     1261 gataacggac agcgatatct ggaagccgct ttgaagagcg atccttcgat cgaatacgtc
     1321 catatgccgg gccatgaagt gagtgagcgg ttccccttca cgatggagga gctgaaggag
     1381 tgggatgtga tcatcatcag cgacatcgga tcaaactcct tccttctttc gaacaaggtg
     1441 ttccttgagg gaaagaagga ggccaatcgg cttcagctgc tgaaggagtg gacttcaggc
     1501 ggcggagcct tggtgatgtg cggcggatat ctttcgtttt ccgggtttca agcgagcgcg
     1561 aagttcttcc ggaccccgat cgaagaggtg cttccggttg acatctacac gtttgacgac
     1621 cgggtggaga cgcctgaagg aaccgagata acgatcgtcg atcccaagca cccgatcgtc
     1681 aaagacatca aaggtccctg gccgaacttc ctcggctacc aggaagcacc gttgaagagt
     1741 gacgcccacc tgatcgctca aagccagtat ggacaccccc tgatcgccgt gcgaaagtac
     1801 ggaaagggaa catcgatggt ctggatgagt gacatcggcc cgcactggtg tccgctggag
     1861 ttcacgcaat gggaagggta tgcaacgttc tggaggaacg cagtccacta tctggctaac
     1921 gacttgtaaa ggccttttta aggaagcaat ccacaagatc cgggtccaac accgtcccct
     1981 tcatgccctt caggcttgcg actgcgactg atttgggagt ttgttcagag gtggagttgg
     2041 ggtcggtgag gtttgcccat gcattcgcca ctgcgatgat tcgggcgtag agcgggatct
     2101 cctcgccttt gagccctcgg ggatagccat tcccgtcaaa ccgctcgtga tgatagagca
     2161 ccgcctcggc aagcggggcg tattcatcca atgagcggag gatgttatac ccgatctcgg
     2221 ggtggcgcct cagctccaga gtttgaacct gcgtgagctg ggaggttttc aggcggagga
     2281 tgttcttatt aatcgcgatc ttccccagat catggaagag ggcggctttt tcgatcagtt
     2341 cgacctcttc actgcttagt tgggtctgca tcgccagatc cttggcatag gaggagacca
     2401 gtttgttgtg ctcctcgatc gactcctctt tttcataaag ctgatccaag atggaaacga
     2461 tcatctgccg tttgtacgtg acattctcct tgagcttgtt ccggtacatc cggtcttccg
     2521 cggtcttgaa cgtcgtttca aagccttctt cagtgttgtg ctgagcggaa ccgaacgcca
     2581 cgctgatgag caggttccgc accttctctt ttttcagcgc attgttgatc cgattgagaa
     2641 tcgtctcggc gtgctcggca tcggtctgcg gcagcaggag aacgaactcg tcacctccca
     2701 ctcggaagat gat
//
LOCUS       DAOPUZ010000149         2707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_39180_length_2707_cov_3.177602, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000149 DAOPUZ010000000
VERSION     DAOPUZ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39180_length_2707_cov_3.177602"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PKN79_03275"
     CDS             complement(<1..746)
                     /locus_tag="PKN79_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNZ94815.1"
                     /translation="MSKMVGDLLAQTNEDFEKAKSRGRIQSILSSLVRKNSDLLSLYA
                     VTDLIKPKGETYRGMQTIPVKNIIGSEGRYQDFSLAFYPKKELLRARWRSIDLATKKY
                     VILPPISVFKLGEWYFVRDGNHRVSVAKTQGVEFIDAEVVELDSEIPLEPGLTMKQLR
                     RRVVYYERKRFLEQYDVDCLPMDRIMFSNAGSYAELVNHILVHKYFLNEDSDRELTFK
                     EGAISWYTTVYEPIIAEVRKQGVLTSFPGNT"
     gene            complement(706..1431)
                     /locus_tag="PKN79_03280"
     CDS             complement(706..1431)
                     /locus_tag="PKN79_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ94816.1"
                     /translation="MKILCISDETDPLIYSEHITTRYGDVDLVLSAGDLPLKYYEFII
                     SMLNKPLYFVFGNHNQEYLHQYLSNNQSPSEAFHQQVVGLQPFGGEFVDGRVRYDKRN
                     KLIIAGLGGSMRYNNGDHQFTDFQMFLRIMKMIPRMIFNRIVRGRYVDVLLTHAPPLG
                     LGDGIDLPHKGFSSLLVFMKWFKPAYLLHGHVHLVDFNANRVHLFRQTKVINIFRNYL
                     LEDDQLGGETDVQDGWRPARPDK"
     gene            complement(1434..2132)
                     /locus_tag="PKN79_03285"
     CDS             complement(1434..2132)
                     /locus_tag="PKN79_03285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ94817.1"
                     /translation="MKTMLISGGTSTIGEAAVRYFTQKGWHVYFGYNTGKKKAEALEA
                     ELGPSAAALQLNVQDKDSIKTLASSIDELDLLINNSGIFSSFLTEALEDAEVGRIFDV
                     NVTGLFRLTKAVLPKLKEGASIINIASVNALMPTFGSTTHYDASKGAVIAYTRSLAAE
                     LAPKIRVNALAPGLVAAPYLDEKNEIRKIFEKRSVLKRMVTSEEVAHALLFMAENTAL
                     TGAIIPLDCGYTIG"
     gene            complement(2129..2542)
                     /locus_tag="PKN79_03290"
     CDS             complement(2129..2542)
                     /locus_tag="PKN79_03290"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNZ94818.1"
                     /translation="MNLKEYAKTFDLLPLEGEGGFFRRVHTFKDGSVIYYALDEEDFS
                     YTHRLVTEEVWFHLDGSPVTIVLIDPEGNEEEHLLDKSNPILVVPGLWWQSARPKEGA
                     ALCATVMSPAWNEEEFTLAEEAFVNLHPKTRRLVQ"
BASE COUNT          602 a          742 c          740 g          623 t
ORIGIN      
        1 gtgtttccgg ggaaggaggt cagcaccccc tgcttccgga cctcggcgat gatcggttcg
       61 tagacggtcg tataccagga gatcgctcct tccttgaagg taagctcccg gtcgctgtcc
      121 tcattgagga aatacttatg gaccaggata tggttcacca gctcggcgta ggatccggcg
      181 ttggagaaca tgatccggtc catcggaagg caatcgacgt catactgctc cagaaagcgt
      241 ttgcgttcat agtacacgac ccttcggcgc agctgtttca tcgtcaaacc cggctcgagg
      301 gggatctcgc tgtccagctc gacgacttcg gcatcgatga actcgacgcc ctgagtcttg
      361 gcgacggaga cccggtggtt gccgtcccga acgaaatacc actctccaag cttgaacacg
      421 gagatcggcg gaagaatcac atacttcttc gtcgccagat cgattgaacg ccatcgggcg
      481 cgaagaagct cttttttcgg atagaacgcc agggagaaat cctgatagcg tccttcgctt
      541 ccgatgatat tctttaccgg aatcgtctgc atcccccggt atgtctcgcc cttgggtttg
      601 atcagatccg ttaccgcata gaggctcaac aaatccgagt tcttgcggac cagagaagag
      661 aggatcgatt gaatgcgacc tctgctcttg gctttttcaa aatcttcatt tgtctgggcg
      721 agcaggtcgc caaccatctt ggacatctgt ctctcctccg agttgatcat cttcaagcag
      781 atagtttctg aagatgttga tcaccttcgt ctgcctgaag agatgaacac ggtttgcatt
      841 aaagtcaacc agatgcacgt gcccgtgcaa gaggtaagcc ggtttaaacc acttcatgaa
      901 taccaacaat gaggaaaatc ccttgtgcgg caaatcgatt ccgtccccga gcccgagcgg
      961 cggggcatgg gtaagcagga catccacata ccggccccgg acgattcgat tgaagatcat
     1021 ccgagggatc atcttcatga tccgcaggaa catctgaaag tcggtgaatt gatgatcgcc
     1081 gttgttgtag cgcatcgaac cgccaagacc tgcgatgatg agcttgttgc gcttgtcgta
     1141 gcgcacccgc ccatcgacga actcgccgcc gaacggctgc agtccgacga cttgctgatg
     1201 aaacgcctcg gaaggtgact gattgtttga aaggtattga tgcagatact cctggttgtg
     1261 attcccgaag acgaagtaga gaggcttgtt caacatggag atgatgaact cataatactt
     1321 aagcggcaga tcgcccgccg agagaacaag atccacatct ccatatcggg tggtgatgtg
     1381 ctccgagtag atcagaggat cggtttcatc gctgatgcaa agaatcttca tgcctaccct
     1441 atagtatacc cgcaatcgag gggaatgatc gccccggtga gggcggtatt ttccgccatg
     1501 aagagcaggg cgtgggcgac ctcctcgctt gtcaccatcc gcttcaggac gcttctcttc
     1561 tcgaagatct ttctaatctc attcttttca tcgagatacg gagcggcgac gaggcccggg
     1621 gcaagggcgt tgacccggat cttcggggca agctcggccg ccaggcttct ggtgtacgcg
     1681 atgaccgcgc ctttggaggc atcatagtgg gtcgtcgagc cgaaggtcgg catgagcgcg
     1741 ttgaccgagg cgatgttgat gatcgaggct ccctccttca gtttggggag aaccgctttg
     1801 gtcagccgaa agaggcccgt gacattcaca tcaaagattc tgcccacctc cgcatcctcc
     1861 agcgcttccg tcaaaaagga ggagaagatt ccgctgttgt tgatcagaag atccagctcg
     1921 tcgatcgatg atgccagagt cttgatcgag tccttgtctt gaacgttcag ctggagggcg
     1981 gcggcggaag ggccaagttc cgcttcaagg gcttcagcct tctttttgcc ggtgttatac
     2041 ccaaaataca catgccaccc tttttgagtg aaataccgga cagccgcttc cccgatcgtg
     2101 gaggttcctc cgctgatcag catcgttttc attggaccag cctcctcgtt ttcggatgga
     2161 ggttgacgaa cgcttcctcg gcgagggtga actcctcttc gttccaagcg gggctcatca
     2221 cggtggcgca gagcgccgcg ccttccttgg ggcgggcgct ttgccaccag agcccgggaa
     2281 cgacgagaat cggattgctc ttatcaagaa gatgctcctc ttcatttccc tcgggatcga
     2341 tgaggacgat cgtcaccgga gaaccgtcaa gatgaaacca cacctcctcg gtcaccaagc
     2401 ggtgcgtata ggaaaaatcc tcttcatcaa gggcgtagta gatgacgctc ccgtccttga
     2461 aggtatggac tcgccggaag aatccaccct ccccttcaag ggggaggagg tcaaatgttt
     2521 tggcatactc tttcagattc atacgggcca atgacggacg atcgcgtgaa caagccgggt
     2581 aaagtcctct ttcacccgct cgccggtctc catcacctcc tgatgggaaa gccgttgatt
     2641 gagaacatgc gcacgcttgg gatcagctgc atcaccaaca tggctgccgg ggcgctcaat
     2701 caacggc
//
LOCUS       DAOPUZ010000150         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_39401_length_2696_cov_3.785687, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000150 DAOPUZ010000000
VERSION     DAOPUZ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39401_length_2696_cov_3.785687"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..714)
                     /locus_tag="PKN79_03295"
     CDS             complement(163..714)
                     /locus_tag="PKN79_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNZ94819.1"
                     /translation="MLIVYVLLALIVLLVIWGVSVYNRYVRLRNQAEEAESAIDAHLK
                     QRYDLVPNLVETVKGYAKHEEQTLTKVIEARNKAMSAGTLEEKDQLNASFSASLKTLF
                     ALSEAYPDLKANQGFLDLQAQLSKIETQLLSARKYYNAIIKQLNTICEVFPSSIVASI
                     ARFAKKPYLQIEDEARARVDVKF"
     gene            865..1335
                     /locus_tag="PKN79_03300"
     CDS             865..1335
                     /locus_tag="PKN79_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HNZ94820.1"
                     /translation="MKSRHNRLAVVKELIRNNRIDNQDTLLSMLKVEGFTVTQATLSR
                     DLKMLKVGKISDGWSGYYYSLPENDLVSESEKSYIQDVRRGIISIEFSGNFGVVKTRP
                     GHANSVGIALDILAIPEILGTLAGDDTIFIILREGMTKEDLLESFKTRIPDIEE"
     gene            1339..>2696
                     /gene="pgi"
                     /locus_tag="PKN79_03305"
     CDS             1339..>2696
                     /gene="pgi"
                     /locus_tag="PKN79_03305"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608557.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNZ94821.1"
                     /translation="MKYINLTEANAFKALERAPQIGSKDLLTKERIRSCSVPSAQGVA
                     YNYAAMPVTEEVLDLFQALSDELQLTEKYQILLDGAVMNTGEKRLVLHHLTRGQIGKN
                     VMVDGENIGEFYRKELSKVQTFAKEVRSKSLLLSTGKPVANVVQIGIGGSDLGPRALY
                     LALKEWAEKEGKDHIPASFISNVDPDDPSAVLSTLDAEETLFILVSKSGTTLETLTNQ
                     RFVEAWLKKRNPAIDPSKHIVAVTSKTSPLAKSKEVFRSFFFDDYVGGRYSSTSAVGG
                     VILSIAFGFEVFEALLKGAHEADKAALHPHVKENAALLDALIGVYLRNVLHYPTTAIL
                     PYSQGLSRFPAHLQQLDMESNGKQVNRDGEPVAYPTGPVIFGEPGTNGQHSFYQLLHQ
                     GTDIVPLQFIGFSESQRKDDIAVADSTSQEKLVANLVAQIVAFALGKDDDNPNKRFEG
                     NRP"
BASE COUNT          650 a          780 c          712 g          554 t
ORIGIN      
        1 gcttgggaac gaagaactcc gccacgatcc gttccgtaac atcataggag ttgtccggac
       61 ccaccttcac gctcagatcg atctgcctga acgtataatc gccggctgtg agtgatacaa
      121 gcgcaaccaa gaggagaaag gcaaggaaag atcttcgttt catcagaact tcacgtctac
      181 gcgggcgcgc gcctcatcct caatctgcag atacggcttc tttgcaaagc gggcaatcga
      241 agcgacgatg gaagagggaa acacctcgca gatcgtgttg agctgcttga tgatcgcatt
      301 gtagtacttt ctggcggaaa gcagctgggt ctcgatcttc gaaagctgtg cctgaaggtc
      361 caggaatccc tggtttgcct tcaagtcggg atacgcttca ctgagagcga aaagagtctt
      421 cagcgaagcg gagaacgatg cgttgagctg gtccttctcc tccagcgttc ccgcgctcat
      481 cgccttgttt cgggcctcaa tgactttggt aagggtctgt tcctcatgtt ttgcatagcc
      541 tttcaccgtt tcaaccagat tgggaaccaa gtcataccgc tgcttcagat gcgcatcgat
      601 cgcgctttcc gcttcctcgg cctggtttcg gaggcgaacg taacggttgt atacggaaac
      661 tccccagatg acgaggagaa cgatgagagc caacagtaca tatacgataa gcataggaac
      721 ctccaacacg tatggtcatc tttagcttgc gccgatgaat aggacgtgtc aagatgcgtg
      781 accgccttga ttgacaatca agattgctgt atctttattc gtgtccgctg tataaatatt
      841 ctgaacgtaa ggagcgccca gaagatgaaa tctcgtcaca accgcttagc tgtcgtaaaa
      901 gaactcattc gaaacaatcg gatagacaac caggacaccc tcctttccat gttgaaggtg
      961 gaaggattca ccgtcactca ggcgaccctc tcccgcgact tgaagatgct gaaggttgga
     1021 aagatcagtg acggctggtc gggatactac tacagcctgc ctgaaaacga tctggtcagc
     1081 gagtcggaaa aaagttacat tcaggatgtc cgccgaggga tcatctccat cgagttcagc
     1141 ggaaacttcg gtgtggtgaa gacccgcccc ggccacgcca acagcgtcgg catcgcattg
     1201 gatatcttgg ccattcccga aattctcggg accttggccg gcgatgatac gatattcatc
     1261 attcttcggg aaggaatgac gaaagaagat ttgctggagt ctttcaagac ccgcatcccc
     1321 gatatagagg aataatccat gaagtacatc aatctcactg aagcaaacgc attcaaagcg
     1381 cttgaacgcg ctccgcagat cggcagtaaa gacctgctca caaaagaacg gatacgttcc
     1441 tgctccgttc catccgccca gggcgtcgcc tacaactacg ccgcgatgcc ggtgaccgaa
     1501 gaggtccttg acctctttca ggcgttgagc gatgagctcc agctcaccga gaagtaccag
     1561 atccttctcg acggggcggt gatgaacacc ggcgagaagc ggctggtcct tcaccacctc
     1621 acccgggggc agatcggaaa gaacgtcatg gtcgacggag aaaacatagg ggagttttac
     1681 aggaaagagc tttccaaagt ccaaacgttc gccaaagagg ttcgctcgaa atccctcctt
     1741 ctttccaccg gaaagccggt cgccaacgtc gtgcagatcg gaatcggcgg cagcgatctg
     1801 gggccgcggg ccctctacct cgccctcaag gagtgggcgg agaaggaggg taaagaccat
     1861 attcccgcct cattcatctc gaacgtcgat ccggacgacc cgagcgccgt cctttccacc
     1921 ctcgacgctg aagagacgct tttcattctg gtttccaaga gcggaaccac gttggagacg
     1981 ttgaccaacc aacggttcgt cgaagcgtgg ctgaaaaaac ggaaccccgc gatcgacccc
     2041 tccaagcaca tcgtggcggt caccagtaaa accagcccct tggcgaagag caaggaggtc
     2101 tttcgctcct tcttcttcga tgactacgtc ggaggcaggt actcctccac gagcgcggtc
     2161 ggcggagtca ttctctcgat cgccttcggc ttcgaggtct tcgaagcgct cctcaaggga
     2221 gcgcacgaag cggacaaggc tgctcttcat ccccatgtga aggagaacgc cgccctcctc
     2281 gacgccctga tcggcgtgta cctgagaaac gtccttcatt atccgacgac ggcgatcctc
     2341 ccctacagcc aaggactcag ccgcttcccc gcccacttgc agcagctgga catggagtcc
     2401 aacggcaaac aggtgaaccg ggacggagag cccgtcgcct atccgaccgg cccggtcatc
     2461 ttcggcgagc cggggaccaa cggccagcac tccttctatc agctccttca tcagggaacc
     2521 gacatcgtgc cgctgcagtt catcggtttc agcgagagcc agcggaagga tgacatcgct
     2581 gttgcagact cgacaagcca ggaaaagctt gtcgccaacc tcgtcgccca gatcgtcgcc
     2641 ttcgccctcg gaaaggacga cgacaacccc aacaagcgct ttgaagggaa tcgccc
//
LOCUS       DAOPUZ010000151         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_39692_length_2681_cov_4.308454, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000151 DAOPUZ010000000
VERSION     DAOPUZ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39692_length_2681_cov_4.308454"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PKN79_03310"
     CDS             complement(<1..1401)
                     /locus_tag="PKN79_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94822.1"
                     /translation="MKTNESWDLSSVGDISAQKIQRNLRELAVFSANSIRSLKETAFR
                     YEPIVLMEQALEHYHRTVIREWKGDGEAIRISKALISYLANLLQSLSKPLSKNRQVKQ
                     KDWKDIVRLFDDIVRKSIRIVDNTALALKADGTLTDPILLERFQKEASAWVLPPLVDE
                     ELLKQHHRSLLYQLQPYNALISEVFPSHLDGVLQALSTLIETGEHDLLNHTALSEADA
                     KVLARPIASEEFRVDNHRLTQFQQSSLKPFILIDGSYHCFDPERVLIEGYGIIKGAVT
                     TSSGERKEAWERIEKEKRSLLSVTFFTAMLSSMHYTRKVEYEDGFLDALFEDGKKELV
                     IQIPAAHTVFPEDPIQESAAYARALIEEQKAIDLAKTHPAPSVIIDLRAIKSYPVTLI
                     DNALTLSFLQVAALATTWEGANEIKGELGLLPLGDLTPPPRDKESEEQLEAEQPKAGQ
                     TDDEHDPMKEFDEHLED"
     gene            1487..1906
                     /locus_tag="PKN79_03315"
     CDS             1487..1906
                     /locus_tag="PKN79_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNZ94823.1"
                     /translation="MVTYRKELWFHLNKRRGLVNITEAIGEAVTESGIKEGLVLANAM
                     NITASVFINSVEEGLHEDFEKWLEELAPELPYERYRHNTEKEKNADAHLKRTIMGRET
                     VVAITGGKLDLGPWEQIFYYEFDGLRDKRVLIKIIGS"
     gene            complement(1903..>2681)
                     /locus_tag="PKN79_03320"
     CDS             complement(1903..>2681)
                     /locus_tag="PKN79_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ94824.1"
                     /translation="SRLEDAFALPPMNDLEKLINPKTKAILLCSPNNPTGHVYSKEDL
                     ERTLQLCKKHDIFLVVDEVYREFCYDGERFTSILTFEEYADRVVCVDSFSKRYSMCGA
                     RVGMLVSRNKQFLTDTLKLSQARLCPPDIEQVAATAALTTDPSYLVEVKKEYESRRNF
                     LIKALEKIEGVKTTKPKGAFYIIAELPVEDAEDFATFLLRDFDLNGETVMLAPAEDFY
                     ATKGIGRRQVRIAYVLNKEDLGKAVACLAAGLKAYAERGA"
BASE COUNT          610 a          702 c          714 g          655 t
ORIGIN      
        1 gtcttccaga tgctcgtcaa attccttcat cggatcatgt tcatcatctg tttgcccggc
       61 tttcggctgt tcagcttcta attgttcttc cgactcttta tcgcggggag gcggcgtaag
      121 atcgccaagc ggcagcaggc ccagctcccc tttgatctcg ttggccccct cccacgttgt
      181 cgccaacgcc gccacctgaa ggaaggaaag ggtgagcgcg ttatcgatca atgttaccgg
      241 atagctcttg attgccctca aatcgatgat gaccgagggg gcgggatggg ttttcgccaa
      301 gtcgatcgcc ttctgttctt cgatcaacgc ccgagcatag gcagcgcttt cttggatcgg
      361 atcctcggga aagacggtgt gggcggcggg aatctggatc accagctcct tcttcccatc
      421 ttcgaagagg gcgtccagaa aaccgtcctc gtactccacc tttctcgtgt agtgcatcga
      481 agaaagcatt gcagtgaaga aggtgaccga aagcaggctc cgcttctctt tctcaatcct
      541 ctcccacgcc tctttacgct cgccgcttga ggtggtcacc gcccctttga ttattccata
      601 gccttcgatg agaactcgtt cgggatcgaa gcagtgatag gatccgtcga tgagaataaa
      661 gggcttgagc gaggattgtt ggaactgcgt gaggcgatgg ttgtcgactc gaaactcttc
      721 acttgcgatc ggacgagcca gcaccttggc atccgcttca ctgagcgcgg tatggttcaa
      781 caggtcatgc tcgcccgttt cgatcagggt cgacaatgcc tgaagcactc cgtcgaggtg
      841 agatggaaat acttcactga tcagggcgtt gtaaggttga agctgataga gcagagagcg
      901 gtggtgctgt ttcaggagtt cttcatcgac caacggaggc agcacccatg cgctcgcctc
      961 cttttgaaag cgctcaagca ggatcggatc ggtcagcgtt ccatccgcct tcagggcaag
     1021 agcggtatta tccacgatgc ggatcgattt gcggacgatg tcatcaaaga gtcgaacgat
     1081 atctttccaa tccttttgtt tgacctgcct gttctttgaa agcggctttg aaagagactg
     1141 tagaagattc gcaagatagg agatgagcgc ttttgagatt cgaatcgctt caccatcgcc
     1201 tttccattcc ctgataacgg ttcggtggta gtgctcaaga gcttgctcca tcaatacgat
     1261 cggttcatat cgaaacgccg tctctttaag ggaacgaatg gaatttgctg aaaaaaccgc
     1321 gagctcgcga aggttccgtt ggatcttttg ggctgaaata tcgccaacgg aactaagatc
     1381 ccaactctcg tttgttttca cgccttctct cctgaatcca agcatagcat acctgaggtt
     1441 ttgtgacaaa ctccatgata tactggatgt acagaaggag gcctgtatgg tgacatatcg
     1501 aaaagagctt tggtttcacc tcaataaacg ccgaggtctg gtcaacatca ccgaggcgat
     1561 tggtgaggcg gtcactgaaa gtggaatcaa ggaagggctt gttctggcca atgcgatgaa
     1621 tatcacagcg agtgtattca tcaactccgt ggaggaaggc ctccacgaag attttgaaaa
     1681 atggcttgaa gaacttgccc ccgagcttcc ctacgagcgc tatcggcaca atacagaaaa
     1741 ggagaagaac gctgatgcgc acctgaagcg aacgatcatg ggccgggaaa cggtcgtcgc
     1801 gatcaccggt ggaaaactgg acctcggccc atgggagcaa atcttctatt acgaatttga
     1861 cggactgcgc gataaacgag tcttgatcaa gatcatcggc tcttaagcgc ctctctcagc
     1921 atatgctttc aagccggcgg cgaggcatgc aaccgccttg ccaaggtctt ctttattcaa
     1981 tacatacgca atccgcacct gccttcggcc gatccctttc gtcgcataga aatcctccgc
     2041 cggagcgagc atcaccgtct ctccgttgag atcgaaatcc ctgaggagga acgtggcgaa
     2101 atcctcggcg tcctcgacgg gaagctcggc gatgatgtaa aacgccccct tgggcttggt
     2161 ggtcttcacc ccttcgatct tctcgagcgc cttgatgagg aagttgcgcc tgctttcata
     2221 ctcctttttg acttcgacca gataggaggg atcggtggtc aacgccgccg ttgcggcgac
     2281 ctgctcgata tccggcggac acagccgggc ttgggagagt ttgagcgtat cggtaagaaa
     2341 ctgcttgttt cggctcacca acatccctac ccgggctccg cacatcgagt agcgtttgga
     2401 gaatgaatcg acgcaaacga cccgatcggc gtactcttca aaggtcagaa ttgaggtgaa
     2461 ccgttccccg tcatagcaga attcccgata gacttcatca acgaccagga agatgtcgtg
     2521 cttcttgcag agttggagag ttcgctccag atcttctttg gaatacacat gaccggtcgg
     2581 gttgttcgga ctgcacagaa gaatcgcttt tgtcttgggg ttgatgagct tctcaagatc
     2641 attcatcggc ggaagggcaa atgcgtcctc caaccggctg g
//
LOCUS       DAOPUZ010000152         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_40172_length_2659_cov_3.068356, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000152 DAOPUZ010000000
VERSION     DAOPUZ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40172_length_2659_cov_3.068356"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PKN79_03325"
     CDS             <1..790
                     /locus_tag="PKN79_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNZ94825.1"
                     /translation="GPFVAINIGGVPENLIESELFGHEKGAFTGAVSRKIGMFELAGG
                     GTLFLDEIGDMPLSLQVKILRVLQDRKIIRLGGTTELPINARIIAATNKDLEERVRNG
                     SFREDLFYRLNVVRIHIPPLRQRREDIPLLAAGILKRLNREMGHTVTGISADALNRIT
                     EHDFYGNVRELENILERAMIFATEAEIQESDLDLRHSVSLHSQAQQPQELPQSEAKSL
                     KEAEIEAIIHALHRWEGNRTRAAEELGISRRTLINKIAEYNLSL"
     gene            800..2533
                     /locus_tag="PKN79_03330"
     CDS             800..2533
                     /locus_tag="PKN79_03330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNZ94826.1"
                     /translation="MKSLPRWDLTPIYPGPQSAEFLADIELVRTKSAELEARLADEKS
                     DLQINIEAFELILDHMGNLGSYAHALVSTDTTNAVYLKAFNQVEDLLLVLKPLSVRFM
                     RHLAKREDEIKDRGADDPYAYVLQGLLIEQKHLMSDELEEFAADLARSSWDAFSRLQQ
                     SLGSSISAEWEDGRMKTVVELRNMATDGDRAIRKRAFEKELELLEIHEEAFAAALNGV
                     KGATISLNARRGWGSPLERSIFQSRIDSEILSALIATLEEAVPLFRSYLKKKAELLGL
                     ENLAFYDLFAPVGKSAKRYSYEDAQAFIVEHFSRFSEAMGKFAQNAFEKNWIDPEPRK
                     GKVGGAYDTSFPVAKESRILSNFDYTYSGVSTIAHELGHAYHDSKVMQRGGLLANYPM
                     PLAETASIFCEGLIFRGALEEADEEERIALIEAFLQDSTQVCLDILSRFYFEEEVFKR
                     RKEGDITASELNEMMLDAQKRTYGPALDVYHPYMWAVKSHYYRPEFSHYNYPYAFGQL
                     FGLGLSKRSEDDPSAFVNKYDALLSVSGMKSVRDVGLEAGIDLGDRSFWQEGIEVIAR
                     YVEEFKSASPH"
BASE COUNT          606 a          724 c          799 g          530 t
ORIGIN      
        1 tgggcctttc gtcgcaatca atatcggagg cgtaccggag aacctcatcg agagcgaact
       61 tttcggtcat gagaaggggg cattcaccgg agcggtgagc cgcaagatcg ggatgttcga
      121 gcttgccggc ggcgggacgc tttttctgga tgagatcgga gacatgcccc tctcgcttca
      181 ggtgaagatt ctacgagttc tccaggatcg gaagatcatc cggctcgggg gaaccacgga
      241 gctgccgatc aacgcccgga tcatcgccgc gacgaacaag gatcttgaag agcgggttcg
      301 aaacggaagc ttcagggaag acctgtttta ccgcctcaac gtcgtgcgga tccacattcc
      361 gccgcttcgg cagcgaaggg aggatatccc gctgctggcc gccggcatcc tcaaacgcct
      421 caatcgcgaa atggggcaca ccgtcaccgg catttccgcc gatgcgttga atcgaatcac
      481 tgagcatgac ttttacggaa atgtccggga gcttgaaaac attctggaac gggcgatgat
      541 cttcgccacc gaggcggaga tccaggaaag cgatctggat ctgcgccatt cggtgagtct
      601 gcacagtcag gcgcagcagc ctcaggaact tcctcaaagc gaggcgaaat cccttaagga
      661 ggcggagatc gaagcgatca tccacgccct tcaccgctgg gagggaaatc gaacccgggc
      721 cgccgaagag ctcggcatca gccgtcggac attgattaat aaaatagccg aatataatct
      781 ttcactgtag gagaatgata tgaaatcact accccgttgg gatcttactc ccatctatcc
      841 cgggccgcag agtgccgagt tccttgccga catcgagctg gtccgaacca aaagcgccga
      901 attggaagcc cgcctcgccg atgagaagag cgaccttcag atcaacatag aagcgtttga
      961 gctgatcttg gaccatatgg ggaacttggg atcgtatgcc catgcgctgg tgagtaccga
     1021 cacgacgaac gccgtatatc tcaaggcatt caaccaggtc gaggatcttc ttctggttct
     1081 caagcccctt tccgtccggt tcatgcgaca tctggcgaag cgggaggatg aaatcaaaga
     1141 tcggggagcg gatgatccgt atgcgtatgt gcttcaaggt ctcctgatcg aacagaagca
     1201 tctgatgagc gatgaattgg aggagttcgc cgccgacttg gccagaagca gctgggacgc
     1261 attctcccga ttgcagcaga gtctcggatc ttcgatctcg gcggagtggg aggacggacg
     1321 gatgaagacg gtcgtcgagt tgcgcaacat ggccaccgac ggtgaccggg cgatccgaaa
     1381 gagggcgttt gaaaaggagc tggagctgct ggaaatccac gaagaagcgt tcgccgcggc
     1441 tttgaacgga gtcaaaggag ctaccatttc cctcaacgcc cgcagaggat ggggatcccc
     1501 gctggagcgt tcgatctttc aatcgcgaat cgactcggag atcctttccg ccctgatcgc
     1561 cacccttgaa gaagcggtgc cgcttttccg gtcgtacttg aagaaaaagg cggagctcct
     1621 cgggcttgaa aacctcgcct tttacgatct cttcgccccg gtgggcaaga gcgcgaagcg
     1681 ctactcctac gaggacgccc aagccttcat cgtcgagcac ttctcgcggt tcagcgaagc
     1741 gatggggaaa ttcgcccaga acgcttttga aaagaactgg atcgaccccg aaccgcggaa
     1801 aggcaaagtc ggcggtgcat acgacacctc cttcccggtg gcgaaggaat cgaggatcct
     1861 ttcaaacttc gattacacct acagtggcgt atcgaccatc gcccacgagc tgggacatgc
     1921 ctaccacgac tcgaaggtga tgcagcgcgg cggactgctt gcaaactatc cgatgccgct
     1981 tgccgagacc gcgtcgatct tctgtgaagg cctgatcttc cggggagcgt tggaggaggc
     2041 ggatgaagag gagcggatcg ccctcatcga agcgttcctt caggactcga cgcaggtatg
     2101 tctggacatc ctttcccgct tctacttcga ggaggaggtg ttcaagcgcc ggaaggaggg
     2161 ggacatcacc gcaagcgagt tgaacgagat gatgcttgat gcccagaagc gcacctatgg
     2221 tccggcactt gacgtctatc atccgtacat gtgggcggtc aagagccact actatcgccc
     2281 ggagttctcc cactacaact atccgtacgc attcggccag ctcttcggtc tgggactcag
     2341 caaacgaagc gaggacgatc cctccgcgtt tgtcaacaaa tatgatgccc tgctctcggt
     2401 cagcgggatg aagagcgtcc gggatgtcgg cctggaagcg ggaatcgatc ttggcgatcg
     2461 ctccttctgg caggaaggga tcgaggtgat cgcccgctac gtggaggagt ttaagagtgc
     2521 gagtccgcat tgagaaagtg atccaaggcg gtgaagggct cgccgtgccg aaggatgggc
     2581 ggcgagtctt cgttccagcc gttctgcccg gcgaactggt tgagattgag cttgttgaat
     2641 ccaagagcgg gttcagccg
//
LOCUS       DAOPUZ010000153         2651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_40335_length_2651_cov_3.062018, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000153 DAOPUZ010000000
VERSION     DAOPUZ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40335_length_2651_cov_3.062018"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PKN79_03335"
     CDS             complement(<1..853)
                     /locus_tag="PKN79_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94827.1"
                     /translation="MIEGIFVDGLIFSLMVIGILISYRILDFADLTCDGSVATGAAVA
                     TMAIVGGVPISIALVFAFISGVIAGMITAAIHNKLKIPGLLAGILTMTMLYSINLRIL
                     GNKANVPLLRVQTLYTKLPAFFSFFQPEIASLVGTLLVVLVIKSLVDLWFRTDLGVSM
                     GAMGGNEQMVISQGINPEVLKLMGIGLSNGLIALSGGLLAQYQGFADANLGQGMVVQG
                     LAAIMIGEFLFSSNRISLITLRAIFGAIIYKALLFFGRKYGYLVNITPNDFKLLTGIL
                     VIVSLFIA"
     gene            complement(910..1899)
                     /locus_tag="PKN79_03340"
     CDS             complement(910..1899)
                     /locus_tag="PKN79_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ94828.1"
                     /translation="MKRILTTLVLFVVVTTILFAAGQSDKAPKAEYTVGISKLIAHPA
                     LDAAEQGMIDYLTSQGLSISFDRQNANGDVSTASSIAQKFKSDKVSVAVGIATPSAQA
                     LAQVFGPNQSTPVVFSAVTDSAEAGLVAKNIAGVSDKSPVEEQIKLLMDVTGAKRIGN
                     VYASGEANGVVLMEMAKAATEKFGGEFVSAAISNSSEVKMAAQSIIDRVDAIYVATDN
                     AVVSAIASIDDVTMKAKKPLFSADPSPVEGLNVMIAWGFDYYNIGVETGKVIERILKG
                     TEAGSIGTVYITDPAKYELWFNLDTASQLGYTIPQSLQDAAAVLIKDGKHVSR"
     gene            complement(2061..2318)
                     /locus_tag="PKN79_03345"
     CDS             complement(2061..2318)
                     /locus_tag="PKN79_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94829.1"
                     /translation="MSRYRDEDELEDEEDRYFNDWEEEEEDDFDEREAMRFPIDDDEL
                     LDDEEDDELLDDGFMVDGDDSYEDELYEEDDDDFYYYDDDF"
BASE COUNT          636 a          698 c          672 g          645 t
ORIGIN      
        1 gggcgatgaa gaggctcaca atgaccagaa taccggtaag gagcttgaag tcattggggg
       61 tgatgttgac cagatagcca tactttcgac cgaagaagag gagcgccttg tagatgatcg
      121 ctccgaagat tgcccggagg gtgatcaggc tgattcggtt ggaagagaag aggaattcac
      181 cgatcatgat cgcggcgagg ccttgaacaa ccatcccttg accgaggttc gcatcggcaa
      241 acccctggta ctgggcaaga agtccgcccg agagggcgat caatccgttg gaaagaccga
      301 ttcccatcag tttcagcact tcgggattga ttccctggct gatgaccatc tgctcgtttc
      361 ctcccatcgc tcccatcgac acacccaaat cggttcgaaa ccataggtcg acaagggact
      421 tgatcaccag cacgaccaac aacgttccga caagggatgc gatctccggt tggaaaaagg
      481 agaaaaaggc gggaagcttc gtatagaggg tttgaacgcg gagcaagggg acgttcgctt
      541 tgtttccgag gattcgcagg ttgatggagt agagcatcgt catcgtcaaa attcccgcca
      601 gaaggccggg gatcttcagc ttgttgtgaa tcgccgcagt aatcattccg gcgatgactc
      661 cgctgataaa ggcgaaaacg agggcgatgg agattggaac cccgccaacg atcgccatcg
      721 tagcgacagc cgctccggtg gcaacggacc cgtcgcacgt caaatcggca aaatccaaga
      781 ttcgatagct gatgagtatg ccgatgacca tcagcgaaaa aatcaatccg tctacaaata
      841 ttccttctat catctcatac ctctacacag aaaaaccgcc ggcaagccgg cggtctgagc
      901 ttcaacaact tagcggctta cgtgcttgcc gtccttgatc agaaccgctg ccgcgtcctg
      961 aagggattgc ggaatagtat agccaagctg agaagctgta tcaaggttga accacagttc
     1021 atacttcgcc ggatcggtga tgtagaccgt tccgatcgat ccggcttcgg tccctttgag
     1081 gattcgttca atgaccttgc cggtctccac gccgatgttg tagtaatcaa aaccccaggc
     1141 gatcatgaca ttgagtcctt cgacggggct cgggtcggcg ctgaagaggg gttttttggc
     1201 tttcatcgtt acgtcgtcga tgctggcgat cgcactgacg acggcgttgt cggtggcgac
     1261 gtagatcgca tcgacgcgat caatgatcga ttgggcggcc atcttcactt cacttgaatt
     1321 ggaaatcgcg gcactgacga actcgccgcc gaacttctcc gttgcggctt tcgccatctc
     1381 catcaggacc acgccgttgg cttcgccgga ggcatagacg tttccgatcc tcttcgctcc
     1441 ggtaacgtcc atgaggagtt taatctgttc ttcgacgggg ctcttgtcac tgacgccggc
     1501 gatgttcttc gccacgagac cggcttcagc ggaatcggtt acggcgctga agacgacggg
     1561 agtgctttga ttggggccaa agacctgggc gagcgcctga gcggaaggag tcgcgattcc
     1621 caccgccact gagaccttgt cactcttgaa cttctgagcg atcgaagagg cggtggagac
     1681 gtcaccgttc gcattctgtc tgtcaaagga aatcgaaagg ccttgcgagg taagatagtc
     1741 gatcattccc tgctcggcgg catcaagagc ggggtgggcg atcagcttgc tgattcccac
     1801 ggtatattcg gctttagggg ctttatcgct ctgtcctgcg gcaaataaga ttgttgtaac
     1861 tacaacgaac agcactaggg tggtcaaaat ccgtttcata ccggtatcct ccaacagtga
     1921 ttaagtggcg ttgcgttact ataatgggtt gttgaaaaat tgtaaatatc tgtatgcaaa
     1981 actatagata ttcatacata aaatatgcat aaacaaaaaa cacagcccga aggctgtgct
     2041 gcaggagaat gtatcacgat ttagaaatcg tcatcgtaat agtagaaatc gtcatcatcc
     2101 tcttcgtaga gctcatcctc atacgaatcg tctccatcaa ccataaaccc gtcatcaagg
     2161 agttcatcat cctcttcatc gtcgagcaac tcatcgtcat cgatcgggaa tctcatcgct
     2221 tcgcgctcgt cgaagtcgtc ctcttcttct tcctcccaat cattgaagta ccgatcttcc
     2281 tcatcttcca attcgtcttc atctcgatat cgactcatgt cacgctcctt cgtaagacat
     2341 tacatctaca aacatagaag agagtatatc ggttgtcaat tccaaatcac ctgtcttaga
     2401 catcagggta ccccgtatat aagaatttca gggttttcct gtcttgcttt gtccggtatt
     2461 caaaatcttc ccacgcctcg tccgtccaag ccttaatcat ctacactcac ctcatggatg
     2521 gtgcctccgg tcgactccat ccgggcaatt gatttcttga gccttgccat gttttggtcg
     2581 ctgtagaacg ggtcgacggc tacctcaaac gggatgcgct tctctctggc taccttcgtg
     2641 gcgaacatgg t
//
LOCUS       DAOPUZ010000154         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_40575_length_2640_cov_3.383752, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000154 DAOPUZ010000000
VERSION     DAOPUZ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40575_length_2640_cov_3.383752"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PKN79_03350"
     CDS             complement(<1..1378)
                     /locus_tag="PKN79_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ94830.1"
                     /translation="MYQTIPQLFETIVEKYPSVAVQLSKDEKGTFQPITYRQLHDEVY
                     ALAAALSFYGIKRGDRVGLISDNRKEWLASDLAILSLGAADVPRGQDAMTHEVAFILG
                     ITEAEFCFVENIAQLKKVASLLDQLPHLATIAVLDRKTTKEQVRDLSIKSGITVLLYS
                     ELIAGGNLLLQDSKKRKAIQEAIATGDAEEIATIIFTSGTTGEPKGVMLSHRNFVYQL
                     EAIPKLIKGLEPEQRWLSVLPVWHSFERILQYVAVSNASTIAYSKPLGSILLNDLAVV
                     NPHWMGSVPRIWEAVRSGVFASMKKTGGIKAALFFFFTGVAATYAKNRDLLLGRVATF
                     KKRNRFVDIVRSVLPAVLLFPLYKLGNVLVFSKIRAKLGTSFIAGVSGGGSLSESVDF
                     FFDSIGVKLVDGYGLTESAPVVSIRPLQKSIKRTMTGLEGTELRILDDEGQAVPPGEK
                     GVLYVRGPQ"
     gene            1513..>2640
                     /gene="asnS"
                     /locus_tag="PKN79_03355"
     CDS             1513..>2640
                     /gene="asnS"
                     /locus_tag="PKN79_03355"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNZ94831.1"
                     /translation="MKERIVDVLRKDPSDELIAIEGWVRTKRDSKNVCFLEVNDGSSL
                     KGIQIVFDKNTFSDLATLNNVTTGSAVICEGTLVASGGGGQSVELAASALTLVGECPT
                     DTYPLQKKRHTLEYLREIAHLRVRTNTFGAVARVRNTLAWATHKFFQDKNFLYVNTPI
                     ISSSDAEGAGEMFRVTTLDLLNVPKTEDGQVDYTKDFFGKQTFLTVSGQLEGETYALA
                     LKNIYTFGPTFRAENSNTKRHLSEFWMIEPEMAFCDIHGNMALAEEYVKYLFSEVLRK
                     NEEDMEFFSRHYEAEMGTTLQKIIETPFIRLTYTDAVKELAKKNDTFEFPVSWGTDLQ
                     SEHEKYLTEVVFKSPVIVTDYPKEIKAFYMKLNDDEKTVRGM"
BASE COUNT          638 a          701 c          737 g          564 t
ORIGIN      
        1 cctgagggcc gcggacgtac aacacgccct tctcgcccgg gggcacggct tggccttcat
       61 catcaaggat tctgagctcc gttccctcca agccggtcat cgtccgcttg atgctttttt
      121 gaagagggcg gattgaaacc accggagcgc tctcggtcag cccgtatccg tcgacgagct
      181 tgacgccgat cgaatcaaag aagaaatcga cgctttcact caaggagccg ccgccgctga
      241 cgccggcgat gaagcttgtt ccgagcttcg cccggatttt tgaaaacacc agaacgtttc
      301 caagcttgta gagggggaag agcaacactg caggaagcac cgagcgaacg atgtcgacga
      361 agcggttccg cttcttgaac gtcgccaccc tgccaaggag cagatcgcgg ttcttcgcat
      421 atgttgcggc gacaccggta aagaagaaaa agagagccgc tttgatcccg ccggtcttct
      481 tcatcgaggc gaacaccccg ctgcggaccg cttcccagat gcggggaacc gagcccatcc
      541 aatggggatt cacaacagcc aagtcattga gaagaattga accgaggggc ttggagtaag
      601 cgatcgtcga agcgttggaa acggcgacat attgaagaat ccgctcaaag gagtgccaga
      661 ccggcaggac gctgagccac cgctgctccg gctccaaccc cttgatcagc ttgggaatcg
      721 cctcaagctg gtagacgaag tttcgatggc tgagcatgac gcctttgggt tcgccggtgg
      781 tcccgctggt gaagatgatc gtggcaatct cctcagcatc gcctgttgcg atcgcttcct
      841 gaatcgcttt acgctttttg gaatcttgaa gcagaagatt gcctccggcg atcagctcgc
      901 tgtacagaag aacggtaatg ccgcttttga tcgagaggtc ccggacctgc tccttcgtcg
      961 tcttccgatc gagcacagcg atggtggcaa gatgaggaag ctgatccagc agcgaagcga
     1021 ccttcttcag ctgggcgatg ttttcaacga aacagaactc cgcttcggtg atgccgagga
     1081 tgaacgccac ttcgtgggtc atcgcatcct ggccgcgggg aacgtcggcg gctccgaggg
     1141 aaagaatcgc cagatcgctt gcaagccact ccttgcggtt gtcgctgata agtccgaccc
     1201 ggtcgccgcg cttgattcca taaaatgaga gagcggctgc cagggcgtac acttcatcat
     1261 ggagttgacg ataggtgatc ggttggaatg ttcccttctc gtccttgctg agctgcacgg
     1321 cgacggatgg atatttctct acgatggttt caaatagttg gggaattgtt tgatacatga
     1381 agaccctcca agggctgata ctgatattta tatatacatt aatacaaatt gtgtcaatct
     1441 gtatattagt gcttctcatt gacttgtttc acccaaacgc ttcataatag cgcaaagtcg
     1501 agaggaagcg atatgaaaga acgaattgtt gatgtgctga ggaaagatcc ttcagatgag
     1561 ctgattgcga ttgaaggatg ggttcgaacg aagcgggact ccaaaaacgt atgttttctg
     1621 gaggtcaatg acggctcttc attaaagggg atccagatcg tgtttgacaa gaacaccttt
     1681 tccgatctcg ctactctcaa taatgttacg accgggagtg cggtcatctg cgaaggaacg
     1741 ctcgttgcaa gcggtggcgg aggccagagc gttgagctgg ccgcaagcgc cctcacgctg
     1801 gttggcgaat gcccgacgga tacctatccc cttcagaaga agcggcacac cttggaatac
     1861 ctcagggaaa tcgcccacct cagggtgcga accaacacgt tcggagcggt tgctcgggtg
     1921 cggaacaccc ttgcctgggc gacgcacaaa ttctttcagg ataaaaactt cttatatgtc
     1981 aacaccccga tcatctcctc cagcgacgcc gaaggagccg gagagatgtt ccgtgtcacc
     2041 acgctggacc tgctgaacgt gccgaagacc gaagacgggc aggtcgatta caccaaggac
     2101 ttcttcggaa agcagacctt cctgacggtc agcggtcagc ttgaagggga aacctacgcc
     2161 ttggcgctga agaacatcta taccttcggc cccaccttcc gggcggagaa ctccaacacc
     2221 aagcgccacc tctccgagtt ctggatgatc gagccggaga tggcgttctg cgatattcat
     2281 ggaaacatgg cgcttgccga agagtatgtg aaatacctct tcagcgaagt gctcaggaag
     2341 aacgaagaag atatggagtt cttcagccgc cattatgaag cggagatggg taccacgctt
     2401 cagaagatca tcgaaacccc gttcatccgg ctgacctaca ccgatgcggt caaggagctg
     2461 gcaaagaaga acgacacctt cgagtttccc gtctcctggg gaacggatct gcaaagcgag
     2521 catgagaaat accttaccga ggtcgtcttc aagtctccgg tgatcgtcac cgactacccg
     2581 aaggagatca aagcgttcta catgaagctg aacgatgatg agaagacggt ccgggggatg
//
LOCUS       DAOPUZ010000155         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_41122_length_2615_cov_2.385156, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000155 DAOPUZ010000000
VERSION     DAOPUZ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41122_length_2615_cov_2.385156"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKN79_03360"
     CDS             complement(<1..225)
                     /locus_tag="PKN79_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNZ94832.1"
                     /translation="MAQQKNQRVIGIDPGLASTGWGIVHSVSNRLVYLAHGVIETSAK
                     AAHQDRLLKIFTELNAVLELYTPNLAGMETL"
     gene            complement(369..1178)
                     /locus_tag="PKN79_03365"
     CDS             complement(369..1178)
                     /locus_tag="PKN79_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNZ94833.1"
                     /translation="MALTLTEKILSSHLKTGTLKRGNPIGISIDQTLTQDATGTMAYL
                     QFESMGLDRVQNELAVSYVDHNTIQVGFENADDHRYLQSVAAAKGVIFSRPGNGICHQ
                     VHLERFGVPGKTLLGSDSHTPTGGGLGMIAIGAGGLDVAVAMAGGPFHLISPKIIEIR
                     LTGKLRPWVSAKDVILAVLGRYGVKGNVNCIFEYTGEGVKTLQVPERATICNMGAEAG
                     VTTSISMVPDTYTSLDVETTRKVLKLIDKLEEDDDVQNVYSNIDIPEGFEE"
     gene            complement(1199..2383)
                     /locus_tag="PKN79_03370"
     CDS             complement(1199..2383)
                     /locus_tag="PKN79_03370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ94834.1"
                     /translation="MKSNSIQFHTINLASFFSQLAIAMINLTLVYHLRYHYNLPSALI
                     GLAAAMGPTSYLIACLVKGKLSSNLRPRHAIMLSLAGMSGSILLLLSVHHLAITFSCL
                     FLYGMWMSFLWPHVEEWFSRGKEGEELSKAVNGFNVSWSLGVALSSYLAGLLIEQSTI
                     LAFLVSIGFFALVFIGIWTVSTLVPAIRMVPSETTHIETSGLTDRSTPLRFYAWIGIV
                     VLYSGMSIILTIFPLYAHEELLLRESRVGLLLLIRGITSCVSFYLLGRFSFWHFRKSL
                     IFLVQALFALLCFSALRFSSLIFYGIFFFLFGVLFSFAYTQSIFHGSSGAIRRSTRMI
                     IHEVLLTVGTILGSVGGGWFYQKFGFANVLLADGTFGAVAVGGELLFSIYNQRRFRYN
                     GA"
     gene            2452..>2615
                     /locus_tag="PKN79_03375"
     CDS             2452..>2615
                     /locus_tag="PKN79_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNZ94835.1"
                     /translation="MANSAFISAMLAFLIAQLLKPFVNVLFERRFVWHLLFSTGGMPS
                     SHTAGVIALVT"
BASE COUNT          641 a          695 c          703 g          576 t
ORIGIN      
        1 gagggtttcc atgcccgcaa gattcggcgt atagagttcg agcactgcat tgagttcggt
       61 gaatatcttg agaagcctgt cctggtgagc cgctttcgca ctggtttcta taacgccgtg
      121 ggcgagatac acgagccggt tcgacaccga atggacaatg ccccacccgg tagaagcaag
      181 gccgggatcg atcccgatga cgcgctggtt tttttgttga gccatgagaa cgccattgta
      241 gcgggaatcg ggacaaaaaa aaaccccgaa agcggaatcg tagccgccgt tcgggattct
      301 ttcgttgagg cttgccgaag ttcgcaattt cgttgactcg acgggcgccc gtactcgatt
      361 tatatagatt actcttcgaa tccttcgggg atgtcgatgt tggagtacac gttctggacg
      421 tcatcgtctt cttcgagctt gtctatgagt ttgaggacct tgcgggtggt ttcgacgtcg
      481 agggaggtgt aggtgtccgg aaccatcgag atgctggtgg tgacccccgc ctccgctccc
      541 atgttgcaga tcgtcgcccg ttcgggaacc tggagcgttt tcaccccctc tccggtatac
      601 tcgaagatgc agttcacgtt ccccttcacc ccgtatcgcc ccagaaccgc caaaatcaca
      661 tctttggcgc tgacccatgg gcggagtttc ccggtcaagc ggatttcgat gatcttggga
      721 ctgatcaggt ggaaggggcc gccggccatt gcgacggcaa catccagtcc gccggcgccg
      781 atcgcgatca ttccgagtcc gccgccggtc ggggtatggc tgtcgctacc caagagcgtc
      841 ttgccgggga caccgaagcg ctcaaggtgg acttggtggc agattccgtt tcccggacgg
      901 gagaagatca ccccttttgc cgccgccacg gactgcaagt agcggtggtc gtcggcgttt
      961 tcaaagccta cctgaatggt attatgatct acatagctga ctgccagttc attttgaacc
     1021 cggtccaagc ccatcgattc aaactgcagg tatgccatcg taccggtggc atcctgggta
     1081 agggtctggt cgatcgatat tccgatagga tttccccgct tcaacgttcc cgtcttgagg
     1141 tgagagctga gaattttttc cgtcagcgtg agggccatag catcctcctt gcgaatgatt
     1201 acgctccatt gtagcgaaag cgcctttggt tgtatatgct gaaaaggagt tcccctccaa
     1261 cggcgaccgc accgaaagtt ccgtcggcaa gcagcacgtt ggcaaacccg aacttctggt
     1321 agaaccaccc gcccccgacc gaaccgagaa tcgttccaac cgtgagtagc acctcgtgga
     1381 taatcatccg cgtggagcgt ctgattgcac cgctggaacc atggaagatc gattgggtgt
     1441 aggcgaagga aaagagcact ccgaagagaa aaaagaagat cccgtaaaag atgagtgatg
     1501 aaaaccggag agcgctgaaa cacagaagag cgaagagcgc ttgaacgagg aaaatcagcg
     1561 atttcctgaa atgccaaaac gagaatctgc ccaaaagata gaagctgacg caggaagtga
     1621 ttccacgaat caacaggagc agtccgacgc gggattccct cagcagcagc tcctcatgag
     1681 catacagggg gaagatcgta aggatgatgc tcattccgct gtagagcacg acgattccga
     1741 tccacgcata aaacctcagc ggcgtcgatc gatcggtcaa tccgcttgtt tcaatatggg
     1801 tcgtttcgct tggaaccatc cgaattgcgg gaacgagcgt gcttaccgtc caaatgccga
     1861 tgaacaccaa agcgaaaaag ccgatggaaa caaggaaggc gaggatcgtc gattgttcaa
     1921 tcaacaaacc ggcaaggtat gatgagaggg cgactcccaa cgaccaggag acattgaacc
     1981 cgttgaccgc cttgctgagt tcctccccct ctttgccccg gctgaaccac tcctccacat
     2041 gaggccaaag aaaactcatc cacatcccat agaggaagag acacgaaaac gtgatcgcaa
     2101 gatgatgaac gctcaagagc agcaggatcg aaccgctcat ccccgccaga gagagcatga
     2161 tcgcatgacg aggcctcagg tttgaggaga gctttccttt gaccaggcaa gcaatgagat
     2221 acgaggtcgg ccccatggcc gcggcaagcc cgatcagcgc ggaagggaga ttgtagtgat
     2281 atcgaaggtg atagaccagc gtcagattga tcatcgcaat cgcaagctgg ctgaaaaaag
     2341 aagcaagatt tatcgtatga aactgaatcg aattactttt cattcacacg ctcctccatg
     2401 attgcccaag ttaggtaaaa cctatacatt tatgctacga ataacaacct cctggcaaac
     2461 agtgctttta tctctgccat gcttgctttt cttattgctc aacttttgaa gccgtttgta
     2521 aatgtactct ttgaacgaag gtttgtctgg catctgctct tttccaccgg cggaatgcct
     2581 tccagccata ccgccggggt gatcgccttg gtcac
//
LOCUS       DAOPUZ010000156         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_41999_length_2579_cov_3.811807, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000156 DAOPUZ010000000
VERSION     DAOPUZ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41999_length_2579_cov_3.811807"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PKN79_03380"
     CDS             <1..577
                     /locus_tag="PKN79_03380"
                     /inference="COORDINATES: protein motif:HMM:NF027968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ94836.1"
                     /translation="ARLAALFQLTQRGMIILYYGQELGLPDSKLPLYKRLFRKKRAKQ
                     TLGPMIWSTGEGSGFSMAEPWIPFARGANRLSVENQLLEEGSILQLYRTLIKLRNRTP
                     ALQRGVCSFIQTSNPRLLAYTRETEEERYAVLLNFDERYQRLDVDGVEIRGTVIFSTL
                     RGMASESKGVVLKELGSYEGVILRIGSEAEE"
     gene            complement(560..>2579)
                     /locus_tag="PKN79_03385"
     CDS             complement(560..>2579)
                     /locus_tag="PKN79_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNZ94837.1"
                     /translation="RQLKEVQRGQREMNEELADYIASAMKQWALNRGATHFCHWFQPL
                     TGLTAEKHDSFISPTKGGKVLLEFSGKELIKGEGDASSFPNGGLRETFEARGYTAWDT
                     SSPAFLKEVHGIKTLYIPTAFFSYKGEALDKKVPLLRSNQAIEKQALRVLRALGNTTA
                     KRITVNIGPEQEYFLVDRKYYEQRTDLRLAGRTVIGSLGAKSQEQNDHYYGTIGDRVA
                     VFMSELNYELWKLGISSKTQHKESAPNQFEIAVVYDAANLSTDHNQLLMDVLQMVALR
                     HGMVALLHEKPFAGVNGSGKHDNWSLATEDGTNLYAPGTNVEDNVQFLIFLTSFIKAM
                     DEYAPLIRSGAATAGNDLRLGSSEAPPAVISIYLGDQLTTILETLSKEGNCEKRVREV
                     VKMGTTILPELPKDLTDRNRTSPIAFTGNKFEYRMVGSSQSMAGPNIFINAAVAQVLG
                     EAADRLEAATDKEAESHKIISDFYQNHKRIIFNGNGYSEEWAKEAKKRGLPHYRDTVS
                     ALPQMASEKSLALLEGQEVLSRGETLSRLEIYLEGYSKQINVEASTMIEMCRKEIIPA
                     MITYLGTLSEAIWKQEALNLDVTKQKDLLTLLNESLVKTMEGADLLHQAVTAAKQIQE
                     NPLAQAKAYRDTVVTRMEELRTHVDLMEIHTDKHIWPIPSYDDLLFRL"
BASE COUNT          481 a          688 c          761 g          649 t
ORIGIN      
        1 ggctcggctg gccgcattgt ttcaattgac tcagcgggga atgatcatcc tctattatgg
       61 tcaggagctg ggactccccg attcaaagct tcctctgtat aagcggttgt ttcgaaagaa
      121 acgtgcgaaa caaactcttg ggccgatgat ttggagcacc ggtgaaggaa gcggcttcag
      181 catggccgag ccgtggatcc cattcgcccg gggagcaaac cggctttctg ttgaaaacca
      241 attgctcgaa gagggttcga tccttcaact ctatcgaacc ctcatcaagc ttcgtaatcg
      301 gactcctgcg ctgcagaggg gcgtgtgttc cttcatccaa acatccaatc cccgtcttct
      361 ggcctatacg cgggaaacgg aggaggagcg ctatgcggtt ctgctcaact tcgacgagcg
      421 gtatcagcgc cttgatgtcg acggggtcga gataagggga accgtcatct tctcgacgct
      481 tcgaggcatg gcaagcgaat cgaagggcgt ggttctgaaa gagcttggct catatgaagg
      541 agtcatcctc cggatcggct cagaggcgga agagtaagtc atcataagag ggaatcggcc
      601 agatgtgctt gtccgtatga atctccatca gatcgacatg ggttcgtagc tcttccatcc
      661 gggtaaccac cgtatcgcgg tacgcttttg cttgagccaa gggattctcc tgaatctgtt
      721 tggcggcggt aaccgcttgg tggagaagat cggccccctc catggttttg accaatgatt
      781 cgttcaacaa tgtcagaagg tctttttgtt tcgttacatc gagattcagg gcttcctgct
      841 tccagatcgc ttcggaaagg gttccgaggt aggtgatcat cgccggaatg atctcttttc
      901 ggcacatctc gatcatggtt gaagcttcaa cgttgatctg cttggaatac ccttcaagat
      961 agatctccag ccggctcagc gtctcccctc tggagaggac ttcctgacct tccagcagag
     1021 ccagactttt ttcgctcgcc atctggggaa gagcgctgac ggtatccctg tagtggggga
     1081 gcccccgttt tttcgcttct ttcgcccact cctccgagta gccgtttccg ttgaagatga
     1141 ttcgtttgtg gttctgatag aaatcgctga tgatcttgtg cgattccgct tctttgtccg
     1201 ttgccgcttc caggcgatcg gcggcttcgc cgagaacttg ggcgaccgcg gcgttgatga
     1261 agatgttcgg tccggccatc gactgggagg aaccgaccat ccggtattcg aatttgtttc
     1321 cggtaaaggc gatcgggctg gttcgattgc ggtcggtgag atccttcggc agctcgggca
     1381 gaatcgtggt tcccatcttc accacttccc tgacgcgttt ttcgcagttg ccttcctttg
     1441 aaagtgtttc cagaattgtc gtgagctgat cgccgagata gatgctgatg actgcaggcg
     1501 gcgcttcgct ggagccgagg cgcagatcgt tgcccgccgt ggcggcgccg ctgcggataa
     1561 ggggagcgta ctcatccatc gccttgatga aggaagtgag gaagatcagg aactggacgt
     1621 tgtcctccac gttggtcccg ggagcataaa gattcgtgcc gtcttccgtg gcgagcgacc
     1681 agttgtcgtg tttgcccgat ccgttcacgc cggcgaaggg tttttcatga aggagggcta
     1741 ccatcccgtg gcgaagggcg accatctgca ggacatccat caacagctgg ttgtggtcgg
     1801 tggaaaggtt cgccgcatcg tagaccaccg cgatctcgaa ctggttgggc gctgattcct
     1861 tgtgctgggt cttgctgctg atgccgagtt tccacagctc gtagttcagt tcgctcatga
     1921 agaccgcaac gcggtcgccg atcgtgccgt agtagtggtc attctgttcc tggcttttgg
     1981 cgcccagcga tccgatgacg gtccgcccgg cgaggcgaag gtcggtccgc tgctcgtagt
     2041 atttcctgtc gaccaggaaa tactcctgct cggggccgat attgacggtg atccgtttcg
     2101 ccgtggtgtt tcccagcgcc ctcaggaccc gcagggcctg cttctcgatc gcctgattgg
     2161 atcggagcag gggaaccttc ttgtccagcg cttcgccttt gtaggagaag aacgcggtgg
     2221 ggatgtacag cgtctttatt ccgtgcactt ccttgaggaa ggcgggggaa gaggtatccc
     2281 aggcggtgta gccccgggct tcaaatgttt cccgaaggcc tccgttggga aaggatgaag
     2341 cgtcgccttc acctttgatc agctcttttc cgctgaactc gagaagaacc tttccccctt
     2401 tggtggggct gatgaaggaa tcgtgtttct cggcggtcag gccggtcagc ggctggaacc
     2461 agtggcagaa atgggtcgcc ccccggttca gggcccactg tttcatcgcg ctggcgatgt
     2521 aatcggcaag ttcttcattc atctcacgtt gaccgcgctg cacttccttc agctggcga
//
LOCUS       DAOPUZ010000157         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_42136_length_2574_cov_2.472410, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000157 DAOPUZ010000000
VERSION     DAOPUZ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42136_length_2574_cov_2.472410"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PKN79_03390"
     CDS             complement(<1..335)
                     /locus_tag="PKN79_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94838.1"
                     /translation="MEQKTRHLLFTFITALLFFFGLGVYATSLLHPYVSAYLFANIPF
                     FALALGLILSHSVLLKQPLVDLVTEIKAWSWRLALLAAISYLAVMGLFTVIGAIRYPH
                     DYHVFHISL"
     gene            367..>2574
                     /locus_tag="PKN79_03395"
     CDS             367..>2574
                     /locus_tag="PKN79_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNZ94839.1"
                     /translation="MSILGDHLASNAVCVLPTEAARRSHLIEYALTSEDGVIAGDSVL
                     SFDTFRSSFLPAYSDRTPSNTLIRFIFATEFVESGNSISSFYNPDFPESKHQALGVIA
                     SMLPSLLKVLTSEIGTLLPRELFRQLQAVHAAYTEFLNRNNLFEPQYDSVSVPSGWNT
                     EREVRILYSDLESEAAVLYEELGRPSWLKLIPTPKADTPTVDVYANHLQEIRSTLRRI
                     RDLLDEGVATHEIMICLAGSDELLATLEDEAYFYGIPLAVRQGRSPLKYPAGRFFSLM
                     NEVHSDRFTLTSLKKLLLEPAIPWKAEERKHIEEFIRLCLKDTILYGSEARPDYFELR
                     LSNRSLRRWYVTFRDALTAIVQATTVADLVRSLSFFRKRFWEEEEWHGTPQGEVFAHA
                     MNQIEQLDRALKACNRERLDGLYALFLSHLQRTQYVPQQQDEGIGVYRWPQGASITGP
                     YRFFLGLDQEGAEVVDNPFPFLSERVRQTLMKRKELGRYHLQAASLGNSTLSCHRRSN
                     NGEKLIHFFFLEEGTVHPHEESLLLDKDPQSGELNRVLKKEGDQKPTYLQRLSFNRAE
                     IGSLRRRSQENDHTWSPIREHLRGLPYEEDGRLKLSATKIDAFAKCPYIYLASYLYKI
                     EPFDYRETLINNKAIGVLLHDAYERFFSSIGPFDSGKIESYEIGLQTAFDHALLKTYG
                     TKGPTASIRQWIIHTFRPKILKILEEEKKYFEHLGTTDTEKELAWKGDRVILDG"
BASE COUNT          614 a          703 c          702 g          555 t
ORIGIN      
        1 cacaatgata tgtgaaatac gtggtagtca tggggatagc ggatcgcgcc gatcaccgtg
       61 aagagtccca tcaccgccaa ataggaaatc gccgccaaga gggcgaggcg ccaggaccac
      121 gcttttattt cggtgacaag atcgacgagc ggctgtttca ggagaaccga gtgggaaagg
      181 atcagcccga gagcaagggc gaagaagggg atgttcgcga aaaggtatgc cgagacatat
      241 ggatgcagca gggaggtcgc atagactccc aagccgaaaa agaacagaag agccgtgatg
      301 aaggtaaaca gcaggtggcg tgttttttgt tccattgttt tgtaaatgta cggtatactg
      361 caatggatgt ctattcttgg cgatcatctc gcatcaaacg cagtgtgcgt ccttcctact
      421 gaagcggcaa ggcgaagcca cctcattgag tatgctttaa cgagtgaaga cggggtgatc
      481 gccggcgatt ccgttctgag ctttgatacg tttcgctcct cttttttgcc ggcctattcc
      541 gaccggaccc cctcaaatac gttgatccgc ttcatcttcg ccacggagtt cgttgaaagc
      601 ggcaactcga tttcctcctt ttacaatccc gattttcctg aatcaaagca tcaggccttg
      661 ggcgtcatcg cttcaatgct tccctccctc ctgaaggtgc tcacttcgga gatcggaacc
      721 ctcctgccaa gggagctgtt tcgacaactt caggcggtgc atgccgcata cacggagttc
      781 ctcaatcgaa acaacctgtt tgaaccccaa tatgattcgg tatccgttcc gagcggttgg
      841 aacacggagc gggaggttcg aatcctctac agcgatctgg agagtgaagc tgccgtcctg
      901 tatgaggagc tgggcaggcc aagctggctg aagttgatcc caacgcccaa ggcggatacc
      961 cctacggttg acgtgtatgc gaaccatctt caggagattc gttcaaccct ccgccggatt
     1021 cgagacctgc tcgatgaggg ggtggcgacg catgagatca tgatctgtct ggcaggcagc
     1081 gatgagctgc ttgcgacgct ggaggatgag gcatacttct acggcattcc gcttgcggtc
     1141 cgtcagggtc gttcaccgct gaagtatccc gccggtcgct tcttctcctt gatgaacgaa
     1201 gtgcacagcg atcggtttac cctgacgagc ctgaagaagc tcctcttgga accggccatc
     1261 ccttggaaag cggaagagcg aaaacacatt gaggaattca tccgcctctg cctgaaggat
     1321 acgatcctct atggatcgga agcccgcccc gactacttcg aattgcgcct gagcaatcgt
     1381 tccttgagaa gatggtatgt gacgttccgc gatgcgctca ccgccatcgt tcaggcaact
     1441 acggtcgccg atctggttcg ttcgctttcc ttctttcgca agcgattctg ggaagaagag
     1501 gaatggcatg gaacccctca gggggaggtg ttcgcccatg ccatgaacca gatcgaacag
     1561 cttgaccggg ctctcaaagc gtgcaaccgt gaacgtctcg acggtctgta cgcccttttt
     1621 ctctcccacc tgcagcggac gcagtacgtt ccccagcagc aggatgaagg aatcggggtc
     1681 tatcgctggc cgcaaggggc gagcattacc ggtccatatc gctttttcct tggactcgac
     1741 caagagggag cggaagtggt cgataatccg ttccccttcc tttccgaacg ggttcgacaa
     1801 accctgatga agcggaagga actcggacga tatcacctcc aagcggcttc cttgggtaac
     1861 agcaccctgt cctgccaccg ccgctccaac aatggggaga agctcatcca cttctttttc
     1921 ctcgaagagg gaacggttca cccacacgag gaaagccttc ttctcgacaa ggatccgcaa
     1981 agcggcgagc tgaatcgagt tctgaagaag gaaggtgatc agaaaccgac ctacctgcag
     2041 aggctaagct tcaaccgtgc ggagattggc tcgctgcgcc gtcggagtca ggaaaacgac
     2101 catacatggt ctcccatcag ggaacacctc agagggcttc cctatgagga agatggacga
     2161 ctcaaactct ccgccacgaa aatcgatgcc ttcgccaagt gcccgtacat ctatctggcg
     2221 agctacctgt acaagatcga gccgttcgac taccgggaaa ccctgatcaa caacaaggca
     2281 atcggagtgc tgctgcacga tgcctatgaa aggtttttct cctcgatcgg gcctttcgac
     2341 agcgggaaga tcgaatccta tgagatcggt cttcaaacag cgttcgacca tgcactgctg
     2401 aagacgtacg gaaccaaggg acccaccgct tcgatccgcc agtggatcat ccataccttc
     2461 cgtcccaaga tcctcaagat tttggaggag gaaaagaaat attttgaaca cctcggaacc
     2521 accgacacgg agaaggagct tgcatggaaa ggagaccggg tgattctgga cggg
//
LOCUS       DAOPUZ010000158         2568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_42299_length_2568_cov_2.570633, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000158 DAOPUZ010000000
VERSION     DAOPUZ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42299_length_2568_cov_2.570633"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PKN79_03400"
     CDS             complement(<1..759)
                     /locus_tag="PKN79_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ94840.1"
                     /translation="MKIAVLSGKGGTGKTLVSVNLAAVAEKGGYFDCDVEEPNGHLFL
                     KPSDIEEEEVTSLKPVVDMDKCDGCRVCVDACVYKALAYIPGELMIFDEICHACGLCT
                     HLCPRQALTETKRVIGTIRRGRRGSLSVHTGTMHIGESTGVPIIKELLKKLDDDLSII
                     DSPPGSGCLVLETIAHADYCILVAEPTIFGAQNLALVHELTQVMNKPAGVLLNKVEDG
                     INPSELYAKEKTLKILGSLPYDSYVALENSNAELL"
     gene            complement(763..1125)
                     /locus_tag="PKN79_03405"
     CDS             complement(763..1125)
                     /locus_tag="PKN79_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNZ94841.1"
                     /translation="MIIAIPAEEKSLDSVISVNFGRSPYYCIYDTEKEQSTFVENKAV
                     ERSGGAGVEAAQNLVDRKIDALVTFRLGDHAAKVLTTAKIKVLKAVNLSIADNIAKVL
                     DHSLEELNEVHPGGINKG"
     gene            complement(1125..1493)
                     /locus_tag="PKN79_03410"
     CDS             complement(1125..1493)
                     /locus_tag="PKN79_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNZ94842.1"
                     /translation="MSRPRKFRNVCSLPQVTEFGPLAGPPNRGDAIILSVDEYETLRL
                     IDTVGMTQEECALEMGVGRSTIQGIYNVARRKVSTALVEGKILSIRGGDYRLREHRGG
                     GHGCGRGCGQGRRRGQRGEW"
     gene            complement(1490..1741)
                     /locus_tag="PKN79_03415"
     CDS             complement(1490..1741)
                     /locus_tag="PKN79_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNZ94843.1"
                     /translation="MPYRDGTGPMGYGPMSGRGMGPCRREVGYSRGFGRGRRFGIGMG
                     MGMGWGLGAQPYTLADRKRVLQEELKRIDELLEQEGKEE"
     gene            complement(1870..2085)
                     /locus_tag="PKN79_03420"
     CDS             complement(1870..2085)
                     /locus_tag="PKN79_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HNZ94844.1"
                     /translation="METQEQRVMILHGFSREELYMAIRAVKTVLPDADVAFAKSTEHS
                     LKRTLGELVGEIAEDHAYMKANPPDQE"
BASE COUNT          535 a          702 c          688 g          643 t
ORIGIN      
        1 gaggagctcg gcgttggagt tctccagcgc cacatatgaa tcatagggca gggacccgag
       61 aatcttcaag gttttttcct tggcgtagag ctccgacgga ttgattccgt cttcgacttt
      121 gttgagcaat acccccgccg gcttgttcat cacctgagtc agctcatgga ccagggcgag
      181 gttctgagcg ccgaagatcg tcggctcggc gaccagaatg cagtagtcgg cgtgggcgat
      241 cgtctcgagg accagacacc ccgagcctgg ggggctgtcg ataatggaaa gatcgtcatc
      301 gagttttttc aggagttcct tgatgatcgg gaccccggtg ctttcgccga tgtgcatcgt
      361 tcctgtatgg acggaaaggg agccgcgtct gccccgccgg atcgtcccga tcacccgttt
      421 cgtctccgtg agcgcctgcc ggggacacag gtgggtgcag agcccgcagg catggcagat
      481 ctcatcgaag atcatcagtt cgccggggat gtaggcgagg gctttataca cgcacgcatc
      541 gacgcagacc ctgcatccgt cgcatttgtc catatccacg acgggcttga gggaggtgac
      601 ctcctcttct tcaatgtcgg acggcttcag gaagaggtgt ccgttgggct cttcgacatc
      661 gcagtcgaag tatcctcctt tctccgctac agcggccagg ttcaccgaaa cgagggtttt
      721 gcccgttccg cctttgccgc tgagaaccgc tatcttcata tatcatcctt tgttgatccc
      781 gccggggtgg acttcgttca gctcctcaag ggaatgatcg aggactttgg cgatattgtc
      841 ggcgatcgaa aggttcaccg ccttcagcac cttgatcttc gctgtggtga gcaccttcgc
      901 cgcatgatcg ccgaggcgga aggtgacaag ggcgtcgatc ttccgatcca ccagattctg
      961 ggcggcttcg actcccgctc caccggagcg ttcgacggct ttgttctcga cgaacgtcga
     1021 ctgttctttt tccgtgtcat agatgcaata atagggactg cgtccgaagt tgacgctgat
     1081 gacgctgtct aaactttttt cttctgccgg aattgcgata atcattacca ttctcctctc
     1141 tggccgcgcc ttctgccttg gccgcagccg cggccgcagc catgaccgcc tccgcggtgt
     1201 tcgcgcaggc gataatcgcc gccccggatg gaaaggattt ttccttcgac cagggcggtc
     1261 gatactttcc gtcgagcgac gttgtagatt ccttgaatcg tcgatcgacc cacgcccatc
     1321 tccagggcgc attcttcctg agtcatcccc accgtgtcga tgaggcggag agtttcatac
     1381 tcgtccacgc tcaggatgat cgcatcccca cgattgggcg ggcccgcaag gggcccgaac
     1441 tcggttactt gaggaaggga gcaaacgttg cggaacttcc tcggtcggct cattcttcct
     1501 tgccttcctg ctcaagcagc tcatcgatcc gcttcagttc ttcctgaaga acccgcttac
     1561 ggtctgcaag ggtgtagggt tgtgcgccca gaccccagcc cattcccatg cccattccta
     1621 ttccgaatct cctgccccgg ccaaagcccc ggctgtatcc aacctcgcgg cggcaggggc
     1681 ccatgccgcg tccactcatc gggccgtagc ccatcggtcc tgttccgtct ctatatggca
     1741 tatgtgcctc cttatgaatg atgttctctt tctgttaatg gcatatgcca ataactgggt
     1801 gtactgtacc accattattg gcatatgtca ataaccatgg atgaaaatgg ttctaccctg
     1861 atatgtccat cattcctgat cgggcggatt cgccttcata taggcgtggt cttcggcgat
     1921 ctcgcccacc agctctccga gagtgcgctt caatgagtgc tcggtgcttt ttgcaaaggc
     1981 gacatccgca tccggcaaaa cggtcttcac cgcccggatc gccatataca gctcttcacg
     2041 gctgaagcca tgaaggatca tgacccgttg ttcctgtgtt tccatgatca gatggtagcc
     2101 ttggatggag ggaagggcaa gaagaaggga agctgaagaa ggagcgatag atctaccttg
     2161 gaactatgtg gttctgaaat tcaatgttta caataataac attgtaagta caacaaaatg
     2221 ggtggtataa tcacggagac gtacgttgca agagcacagg atggtctcac tttgtcatgc
     2281 gtccttacca tacttacatg aggaggatgg ttgtacatat tagattagtt gattgactta
     2341 tcttatacat tatgtataat ctaggataca gaggtgccaa aaccgcaacc ggaatacgat
     2401 atcaaagatt ttttgaaggc atgtaaggag aacgggcgtc aaccgccgaa agtagtgctc
     2461 atggggggga gttctcagaa cagcatctag cgatttttct cttaagacca aggaagccct
     2521 tcttgcattc atcaatgcag gaggtttgga agatcttgaa tttgtcaa
//
LOCUS       DAOPUZ010000159         2565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_42379_length_2565_cov_3.343825, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000159 DAOPUZ010000000
VERSION     DAOPUZ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42379_length_2565_cov_3.343825"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PKN79_03425"
     CDS             complement(<1..975)
                     /locus_tag="PKN79_03425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ94845.1"
                     /translation="MRSVRVYRYRWVMLISLINLVLAGSLQWLNFAPVGRIVNAYYRD
                     QIHLRFFNPIDLLSLTYLIVYVLFSIPSGWIIRKLGMRVTYWIASGLIIFGAMTKTLY
                     LSSFKTVLFGQILLGLAQTFTLTSITETTSRWFPIRERGVAVGIIQASQYLSLALVMI
                     LSPLFVVRSQKGIEQLMRLWAIISILLAAVSAALVRERPPSPSSTFEREEEMKFSQAL
                     TSAFSRRSLRILIMEFATSWAVLMTVFIKIDEISEFLGFANSNGILAITMLIFGMVSA
                     VVIPALSDRFRKRKVFYTFCALTAVPGSFLLLSGNITVALIGAAILGSG"
     gene            1058..1837
                     /locus_tag="PKN79_03430"
     CDS             1058..1837
                     /locus_tag="PKN79_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ94846.1"
                     /translation="MNETLRLIHNRRSLRAFSDKPIPPEVLLALKEATLRAPTAGNMA
                     YYSIVEVCDREKKEKLALLCDNQPMIAKAPLVWIFLADAQKWLNYYHESGAVEKANDL
                     PYRPIGLGDLHLALQDTIIAAHNAVIAAEALGLGSCYIGDVIENFEELSDLLHLSGSV
                     IPGAMVIFGYPKTDNAVKPTPRCPVDSIFMKDSYREPHLKEMEAAYFEHEEQKRRIQS
                     LPYENTGTLADFYYRRKHTSAFMREMNRSTEVMFRRWVEEE"
     gene            complement(1868..>2565)
                     /locus_tag="PKN79_03435"
     CDS             complement(1868..>2565)
                     /locus_tag="PKN79_03435"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ94847.1"
                     /translation="VLLSDDPDQDLDDYIAVYLRDEIGREGNVRKLETFARFLDVAAL
                     TNAEQVNYMNRAGDVGISRQSVANWYQILIDTLIGHYLPAYTKGVKRKTFSLPKFYLF
                     DLGITRALLQIPPPVEGQSEYGRFLEHYIFMELRAYLDYGQRKEILSYWRTTGGIEVD
                     FILGDRIAIEVKATKQSNTRDYKGLLAFMEEGIAERHILVCREEHPRKLKNGVEIHPW
                     AFFLEQLWGGNIL"
BASE COUNT          640 a          698 c          681 g          546 t
ORIGIN      
        1 cccgctacca agaatcgccg ctccaatcag ggcgaccgtg atgttgccgc tgaggagcag
       61 aaaactgccg ggaacagcgg tgagggcgca gaacgtataa aacactttcc gttttcggaa
      121 gcggtcgctc aaggcgggaa tgacgaccgc cgaaaccatt ccgaagatga gcatcgtaat
      181 cgccaagatt ccgttggaat tggcgaaccc cagaaattca ctgatttcat cgatcttgat
      241 gaaaaccgtc atcagaaccg cccaggatgt agcaaactcc atgatgagga ttctgagcga
      301 gcgacggctg aacgctgagg tcagagcttg gctgaacttc atctcttctt cacgctcgaa
      361 tgtggatgac ggggatggcg gccgctcccg aacgagggcg gcactgacgg cggcaaggag
      421 gattgaaatg atcgcccata gcctcataag ctgttcgatc cccttttggg agcgaacgac
      481 gaagaggggg gaaaggatca tgacgagggc aagagagaga tactggctcg cctgaatgat
      541 tcctaccgcc actccccgct cccggatcgg gaaccagcgg ctcgtcgtct cggtaatgct
      601 ggtaagggtg aacgtctgag cgagaccgag gaggatctgg ccgaacagaa cggtcttgaa
      661 ggaggagagg tacagggtct tggtcatcgc cccgaagatg atcaggccgg atgcgatcca
      721 ataggtgacc cgcatgccga gcttgcggat gatccagccg gaggggattg agaagaggac
      781 gtagacgatc aaataggtaa gggagaggag atcgatcggg ttaaagaacc ggagatggat
      841 ctggtctcgg taataggcgt tcacgatccg ccccacagga gcgaagttga gccactgcag
      901 gcttccggcg agaacaagat tgataaggct gattaacatt acccaccggt agcggtacac
      961 cctgacgctt ctcatgatct tcatcataca ataccagggg catatctgta tatccgggtg
     1021 gtggtacaag cgactctctt ttcctatact tgtcaccatg aatgaaacac tccgcctgat
     1081 acacaatcgt cgttcgctcc gagctttttc cgacaaaccg atcccgcctg aagtcctgct
     1141 tgccctcaaa gaggcgaccc tcagggctcc taccgcgggg aacatggcgt actattcgat
     1201 cgtcgaggtc tgtgaccggg agaagaagga gaagcttgcc ctcctctgcg ataaccagcc
     1261 gatgatcgcc aaagctccgc ttgtatggat ctttcttgcc gatgcccaga agtggctgaa
     1321 ctactatcat gaaagcggag cggttgaaaa agcgaacgac ctcccctatc gcccgatcgg
     1381 ccttggcgac ctccacctcg cccttcaaga cacgatcatc gccgcccaca acgccgtgat
     1441 cgccgcagaa gcccttggcc tcggttcctg ctacatcggc gatgtcatcg agaacttcga
     1501 agagctcagc gacctcttac acttaagcgg ttccgtcatc ccgggagcga tggtcatctt
     1561 cgggtacccc aaaacagaca acgcagtaaa accgaccccc cgctgtcccg tcgactcgat
     1621 cttcatgaag gattcctacc gggagcccca cctcaaggag atggaagccg cctattttga
     1681 gcacgaagag cagaagcgaa ggatccaaag cctcccctat gaaaacaccg gaaccctcgc
     1741 cgacttctat taccggcgca agcacaccag cgcgttcatg cgggagatga accgctcgac
     1801 cgaggtgatg ttcagacggt gggttgagga agaatgaaat cattcctcga ttgatcgtga
     1861 gttctcccta aaggatgttt ccgccccaaa gctgctcgag gaaaaatgcc cagggatgga
     1921 tttcaacacc gttcttcagt tttctcgggt gctcttctcg acacacgaga atatgccgtt
     1981 cggcgattcc ttcttccatg aatgcaagca agcctttata atcccgggta ttcgactgtt
     2041 tggtcgcttt cacttcgatc gctatccgat cgccaaggat gaagtcgacc tcgatgccgc
     2101 cggtcgtccg ccagtaggaa aggatctctt tccgctgacc ataatcaaga tatgccctta
     2161 gttccatgaa gatataatgc tcaagaaaac gtccatattc gctttgtccc tccaccggag
     2221 ggggaatctg cagcaatgcc cgcgttattc cgagatcaaa gagatagaac ttcggtaagc
     2281 tgaacgtttt ccgtttgacg cccttggtat atgccgggag gtaatgtccg ataagggtgt
     2341 ctatgaggat ttgataccaa ttggctacgg actgcctgct tataccgaca tcaccggctc
     2401 tgttcatgta attgacctgc tcggcatttg taagagcggc gacatccaaa aaacgggcaa
     2461 atgtttccaa tttacgcacg ttgccctcgc gaccgatctc atctctcaga tagacggcga
     2521 tataatcatc aaggtcttga tcgggatcat ccgacaaaag aacat
//
LOCUS       DAOPUZ010000160         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_42396_length_2564_cov_2.962136, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000160 DAOPUZ010000000
VERSION     DAOPUZ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42396_length_2564_cov_2.962136"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKN79_03440"
     CDS             <1..397
                     /locus_tag="PKN79_03440"
                     /inference="COORDINATES: protein motif:HMM:NF017444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P13 family porin"
                     /protein_id="HNZ94848.1"
                     /translation="GFGNGSVLQYDSMGALTGAVLDGASAITLSVGLGIFIGLISVHP
                     DTFSDHLPLFSVGVDLMVAGMIGLGVSRIVQAIIPAVYGLRYNKVLREGLGLEKDRSD
                     ALRPALGFAPLPTSDGRLQWQVAARVPLQ"
     gene            complement(389..>2564)
                     /locus_tag="PKN79_03445"
     CDS             complement(389..>2564)
                     /locus_tag="PKN79_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNZ94849.1"
                     /translation="NGLSPIAELRRRVYLGAERRYAEVTDQVVHRIEYELSIIEAKHF
                     SSYFLIMDEIVALASRSCGRGSGAASIVSYALGITNVDPIAHNLYFERFLSPGRTDPP
                     DIDVDFAWDERPRIFEAIKDRYGEDRIGRVANHIFYRRRNALRECARAWGQNDRAINR
                     FLERTDSLDPDWRTIRSIADHLAGLPKEIGTHSGGIIITPKPIVTYAPITRTGDGYPL
                     LVWEKDGCELAGFVKLDILGNRSLAVIRDALSNLKADGIVIDEDRWRPIDDVETQDTL
                     ARGDSMGVFYIESPAMRQLQKKTKKGDFDHIVIHSSMIRPAANRFIGEYVKRLNGKPF
                     THLHPRLESILGETYGILCYQEDVSKTAIALAGFDEVEADRLRKIIAKKHATKRLEAY
                     REQFIRGCIYNGVPIQTIERVWAMMLSFDGYSFCKPHSASYAMVSFQSAYLRTHHPAY
                     FLAAVLTNQGGYYHGGAYVSEARRMGLIVEGPDLNRSQRAYIANGNTIIVGLMAIATL
                     SEGAILRIIRERSANGLFHSLEEAASRLHLGREDWQALSQSGALEVLAPGSCRSDQLR
                     VVLTTAYEEHKDAQLELFGQANEILPVRLPPAHRTLGELHAEYEALGFLRTAHPLILY
                     ATHLQRIRRNLAKDLPCHIGRRVTLVGYPITLKRVFTSAREVMAFFSFEDETALFETV
                     LFPEIYRRYERLLGSSRPLVIQGVVESDEGALTVEVDRLSSL"
BASE COUNT          512 a          727 c          767 g          558 t
ORIGIN      
        1 cgggttcggc aacggttcgg ttctccagta cgattcgatg ggagcgctca ccggggccgt
       61 cttggacgga gcgagcgcga tcacgctgag cgtcggactg gggatcttca tcggcctcat
      121 cagcgtccat ccggacacat tttccgacca tctcccgctc ttttcagtcg gcgtggacct
      181 gatggtcgcc ggcatgatcg gcctcggagt gagccggatc gtccaggcga tcattcctgc
      241 ggtctacggc ctgaggtaca acaaggtcct cagggagggg ctcggccttg agaaggatcg
      301 gagcgacgcc cttcggcccg ctctcgggtt tgctccgctt cccacctctg acggccggct
      361 tcagtggcag gtcgccgccc gggtgcccct acagtgagga aagacgatcg acttcaacgg
      421 taagagctcc ctcatcgctc tcgacgactc cttggatgac aagaggacgg gaggagccca
      481 gcagccgttc atatcgccga tagatctcag ggaaaagcac cgtctcaaac aatgccgttt
      541 catcctcgaa ggagaagaac gccatcacct ctcttgcgga ggtgaagacc ctcttcagcg
      601 tgatcgggta gccgacgagg gtcacccgcc tgcctatatg gcagggaaga tccttcgcca
      661 gattccgtct gattctttgt agatgggtgg cgtaaaggat gaggggatgg gcggttcgaa
      721 gaaatcccag agcttcatat tcggcatgaa gctcgccgag agtgcggtgg gcggggggaa
      781 gtcgaaccgg cagaatctcg ttcgcttgtc caaaaagctc aagctgagca tccttgtgct
      841 cctcgtatgc ggtagtcaga accaccctca gctgatcgct tcgacaggag ccgggggcga
      901 gtacctccaa ggctccgctt tgacttaagg cctgccagtc ctcccggccg aggtgcaagc
      961 gggaggccgc ctcttccaac gagtgaaaca agccgttggc ggagcgttcc cggatgatcc
     1021 gaaggattgc cccttcactc aaagtcgcta tcgccatcaa accgacgatg atcgtattcc
     1081 cgtttgcgat atatgcccgt tggctccggt tgagatccgg cccttcgacg atgaggccca
     1141 tccgtcttgc ttcggagaca taggctcccc cgtggtaata cccgccctga ttggtcagca
     1201 ccgcagcgag gaaatatgcg ggatgatggg ttctgagata tgcgctttga aaggagacca
     1261 tcgcgtacga agcgctgtgc ggcttgcaaa aggagtagcc gtcgaagctc agcatcatcg
     1321 cccagacccg ttcgatcgtt tgaatcggaa ccccgttgta tatgcatccc cggatgaatt
     1381 gttcccgata cgcttcaagg cgcttggtcg catgtttttt cgcgatgatc ttacgcagcc
     1441 ggtccgcttc gacctcatca aaacccgcca aggcgatcgc tgtcttcgag acatcctcct
     1501 gataacagag gattccgtag gtttctccga gaatcgattc aagacgcggg tgaagatggg
     1561 taaacggctt gccgttcagg cgtttgacat attcgccgat gaagcgattg gcggcggggc
     1621 ggatcatcga cgaatggatg acgatgtgat cgaaatcgcc ttttttcgtc ttcttctgga
     1681 gctggcgcat cgccgggctt tcgatgtaga acacccccat cgaatcgccc cgggcgaggg
     1741 tgtcctgcgt ttcgacatca tcgatcggcc gccatcggtc ttcatcaatg acgattccat
     1801 ccgccttgag attggacagg gcgtcccgga tcaccgcaag ggagcggttg cccaaaatgt
     1861 ccagtttcac gaaccccgca agctcgcatc cgtccttctc ccacaccaga agcggatacc
     1921 cgtcgccggt acgggtgatc ggggcgtatg tgacgatcgg cttcggcgtg atgatgattc
     1981 cgccggagtg ggttccgatc tctttcggaa gacctgcgag atgatcggcg atggaccgga
     2041 tcgtccgcca atcgggatct aaggagtctg ttctttcaag aaaccggttg attgcccggt
     2101 cattttgtcc ccatgcccgg gcgcactccc ggagggcgtt tcgccttcgg taaaagatat
     2161 ggttggccac ccgacctatc cggtcctctc catatcgatc ttttattgct tcaaagattc
     2221 gaggccgctc atcccaggcg aaatcgacgt cgatatccgg cggatcggtc cggccggggg
     2281 agagaaagcg ttcgaagtag aggttgtggg cgatcggatc gacgtttgtg atccccaatg
     2341 cgtaggagac gatggaggcg gcgccgctgc cgcgcccaca ggaccgggag gcgagggcga
     2401 cgatctcgtc catgatgaga aagtaggaag aaaagtgttt tgcttcgatg atggaaagtt
     2461 cgtattcgat gcggtggacc acctggtcgg taacttcagc gtaccgtctt tccgcgccga
     2521 ggtagacgcg ccttcggagt tcagcgatcg gggacaaccc gttt
//
LOCUS       DAOPUZ010000161         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_42569_length_2557_cov_3.786571, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000161 DAOPUZ010000000
VERSION     DAOPUZ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42569_length_2557_cov_3.786571"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="PKN79_03450"
     CDS             <1..1390
                     /locus_tag="PKN79_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HNZ94850.1"
                     /translation="VGFRNVEEHVPKRWRYGGDFGGQPTNYDFCLNGIVFPDRSLKPV
                     MSECLKVHQPIHVRSSNPSSGYFTIISEYDFITTKDIALTCSIYNESSSEEYTFGVEL
                     PILQPGGQAQIHIRELQESRVRKMMGSSTTFIRFVCTLKEPAPWAPKDHIVALEQFVL
                     STISKSLHIHAPAYPIKQTDDSLFVETILYKASISSEGFLSALHFEDRPPLLTSPLRP
                     SLYRVPTQNDGLKTYTHLEGIKGFSFYHEKKAMKPWLEHSLNDLVLILEELKIRHNQV
                     KTIHQLKTHTGLDIGSVRQTFTFTLEEVFLHAIFDLSDALAEYPKVGYRCSLTKQEEP
                     VSWFGRGPEENYPDRKTGSLIASYDKTVEELFVPYIVPQDCGVRTETTRLVLEGAKIE
                     GILPFSFSIHPYSPEELWEKRHADDLVVDEVDHLSLDAAVRGVGTATCGPDTLDRYRI
                     PGGIYQMRLRLS"
     gene            complement(1397..>2557)
                     /locus_tag="PKN79_03455"
     CDS             complement(1397..>2557)
                     /locus_tag="PKN79_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ94851.1"
                     /translation="DFSNKILTDECDHPAVKTYKHTEFDRMIDEMKPDVVIVTTVDRT
                     HDYYIIRAMEKGCDVLTEKPITTDEVKAQAILDAQKRTGRSIRVTFNYRYAPHHSKIR
                     ELIRDGAVGEVYSIHFEWLLNTEHGADYYRRWHRNKVNSGGLLVHKSTHHFDLVNFWM
                     DSQPKKVFAMGALNFYGQAAAVKRGVTNFSYRYHNSPYAKDDPFAIDLESSPVLKGLY
                     LDAEEESGYIRDRNVFSDEISIEDTMGVLVKYRNGAILTYSLNSYMPWEGYNVMINGS
                     KGRIEYSAMEKPYINAGGKKSDEGAMVYHHIKVLPLFEAAYDVEVEVREGGHGGGDPA
                     MLDDIFLENPPHDPLKRSADHTAGLRSILTGIAANKSIASGLAIDVDSLLSW"
BASE COUNT          589 a          718 c          658 g          592 t
ORIGIN      
        1 cgttggcttt agaaacgtcg aggaacatgt tccgaaacga tggcgctacg gcggagattt
       61 cggcggtcaa ccgacgaatt acgatttctg cctcaacgga atcgtcttcc ccgaccggag
      121 cctcaagccg gtgatgagtg aatgtctgaa ggttcaccaa ccgattcacg tgaggagcag
      181 caatccatca agcggatatt tcacgatcat cagcgagtat gatttcatca ccacgaagga
      241 tatcgctctg acgtgctcga tctacaatga gtcctcaagc gaggagtaca cgtttggagt
      301 ggagcttccc atccttcagc cgggcggcca ggcgcaaatc cacatcagag aactgcaaga
      361 gagccgagtg cggaagatga tgggatcgtc cacgacgttc atccggttcg tctgcacatt
      421 gaaggagcct gctccttggg cgccgaaaga ccatatcgtc gcacttgagc agtttgtcct
      481 cagcacaatt tcaaagtcgc ttcacatcca cgcccctgcg tatccgataa agcagaccga
      541 tgactcgttg tttgtggaaa cgattctcta caaagcttcg atcagcagtg agggcttcct
      601 ctccgccctt cattttgaag accggccgcc gctgttgaca tcaccgctga gaccgagcct
      661 ctatcgggtt ccgacgcaaa atgacggact gaagacgtac acccaccttg aagggatcaa
      721 ggggttttcc ttctaccacg agaaaaaagc gatgaagccc tggttggagc actctttgaa
      781 cgatttggtc ttaatcctgg aagaactgaa gatcagacac aaccaggtga agacgattca
      841 tcagctcaaa actcataccg gcttggatat agggtctgtt cgtcagacgt tcacgttcac
      901 cctcgaagaa gtgtttttgc atgcgatctt cgatctgagc gatgcgctcg ctgaatatcc
      961 aaaagtcggc taccgctgtt cgctcacgaa acaagaggag ccggtttcgt ggttcggccg
     1021 cggaccggag gagaactatc ccgatcgaaa gaccggttcg ctcatagcct cctacgacaa
     1081 gactgtcgaa gagctcttcg tcccctacat cgttccccag gattgcgggg tccgaacgga
     1141 aacgacccgt ctggttcttg aaggggcgaa gatcgaagga atccttccct tctccttcag
     1201 catccatccc tacagtcctg aggagctatg ggagaagaga cacgccgatg acctcgtcgt
     1261 cgatgaggtc gaccacctga gcctcgatgc ggcggtccga ggggtaggaa cggcgacctg
     1321 cggacccgat acgcttgacc gataccggat cccaggcggc atctatcaga tgagattacg
     1381 gctctcgtaa acgctcctac caagagagca atgaatcaac atcgatcgcc agtccgctgg
     1441 cgatcgattt gttcgcagcg attccggtga gaatcgaccg gagtccggcg gtatgatcgg
     1501 cgcttcgctt aaggggatca tgcggcgggt tttccagaaa aatatcgtcc agcatcgccg
     1561 gatcgccgcc cccgtggccg ccttcccgca cctccacctc gacatcatac gccgcttcga
     1621 agagaggaag aaccttgata tggtggtaga ccatcgcccc ttcatcactc ttctttcctc
     1681 cggcgttgat gtacggcttc tccatcgccg aatattcgat tcgacctttg ctgccgttga
     1741 tcatcacgtt gtacccttcc cacggcatat aggagttcag ggaataggtc agaatcgctc
     1801 cgttcctgta tttcacgaga acccccatcg tgtcttcgat ggagatctcg tcactgaaca
     1861 cgttgcgatc ccggatatag ccgctttcct cttccgcatc gagatacagt cccttcagaa
     1921 cgggactgct ctccaagtcg atcgcaaacg gatcatcctt cgcatagggt gaattgtggt
     1981 aacgatagga aaagttcgtc actccccgct tcactgcagc cgcttgacca tagaaattca
     2041 acgcacccat cgcaaagacc tttttgggct gggagtccat ccagaagttg accaagtcaa
     2101 agtgatgggt cgatttgtga accagcagtc ccccgctgtt cactttattg cggtgccagc
     2161 ggcggtaata atccgctccg tgttcggtat tcagaagcca ctcgaagtgg attgaataca
     2221 cctccccgac cgctccgtcc cggataagct cccggatctt ggagtggtgg ggagcatacc
     2281 ggtagttgaa ggtgacccga atcgatcgtc cggtccgctt ttgagcatcc agaatcgcct
     2341 gggccttcac ttcatcggta gtgatgggct tttcggtcag cacgtcacac cctttttcca
     2401 tcgcccgaat gatgtagtaa tcatgagtgc gatccacggt ggtaacgatg accacatcgg
     2461 gcttcatctc gtcgatcatc cgatcgaact ccgtgtgctt atacgttttg acggcaggat
     2521 ggtcgcattc gtcagtgagg atcttattgg aaaaatc
//
LOCUS       DAOPUZ010000162         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_42579_length_2557_cov_2.837730, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000162 DAOPUZ010000000
VERSION     DAOPUZ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42579_length_2557_cov_2.837730"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PKN79_03460"
     CDS             complement(<1..171)
                     /locus_tag="PKN79_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ94852.1"
                     /translation="MKFRFAHNNFNVIDLGKSIAFYQEALGLKELHRKEAKDGSFIIV
                     FMGDGESKHALEL"
     gene            complement(168..836)
                     /locus_tag="PKN79_03465"
     CDS             complement(168..836)
                     /locus_tag="PKN79_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ94853.1"
                     /translation="MVVTFLQHASFLVECGEAVLLFDYFDGEVTLPENKDLIVFASHR
                     HGDHYNKKIFDLPAKAFILSEDIPTEDIPHGIVVHRMGSHQRIEVEGLVARTLQSTDE
                     GVAFLLEVEGKVIYFAGDLNNWFWEEEGEAYTSWMEREYHKEINRLPSAIDVAFVPVD
                     PRLGKRYGLGALDLLERVSVSYLVPMHMWGEFAVGTALKEAVSLYDVEVLAAQRNGQR
                     WRIA"
     gene            complement(876..1976)
                     /locus_tag="PKN79_03470"
     CDS             complement(876..1976)
                     /locus_tag="PKN79_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94854.1"
                     /translation="METKVIIINKNALQKFAEGYIECFGHAQAMVIAEEDEHLHYALP
                     ILRSLHKEGVEATGHTFKRAAFNKIFPLLDDKPVVPVFLGDDRLLSVGKRVSYKLNRS
                     FAAIPTVLDSPAIASPWRFTWPGAFIPSQPPTLLLVDTELIRKPKILPQAVAEALLLL
                     ATHGEAVLFGEENLRIDLHTLAWGSADEFFEAYLDNSRYSLELLAKALAQGGVVDQEL
                     EYVTRRNRRVPVSLLALGIFHLGEQLFTLSERDAVGHMKKDREELIKRLFLPAEQEAL
                     LAKKPKSFRRRPSLNQVKQTLEANILTFGEIKRKLKGAGCITSVEEGRCSLGELKDEL
                     LRTMLRSPHLGILDYYNELGLIETALTMLEGM"
     gene            complement(1951..>2557)
                     /locus_tag="PKN79_03475"
     CDS             complement(1951..>2557)
                     /locus_tag="PKN79_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNZ94855.1"
                     /translation="DEAWFDHVNELGRELGLRIEHFIISSGLTEIIEGSAIGSRFKKI
                     YACEYYYDVNGVAKWPKLAINYTAKTQFLFRINKGILDVWEDNALNDYTPQELRPVPF
                     ANMIYIGDGLTDVPCMKLVKESGGISIAVHAKGEVDLCHRLMAAGRINFFIEADYTPQ
                     GGLFALMKKILARMSSANALEKTHLLMKKEAMSHGNKGHHH"
BASE COUNT          564 a          740 c          689 g          564 t
ORIGIN      
        1 aagctccaag gcgtgcttcg attccccgtc gcccatgaag acgatgatga acgacccgtc
       61 cttcgcttcc ttgcggtgga gctccttcaa accgagcgcc tcctgataaa atgcgatcga
      121 tttcccaaga tcgatcacgt tgaaattgtt gtgggcaaat ctgaacttca tgcaatcctc
      181 catcgctgac cgtttcgttg ggcggccagg acctcaacat catataacga taccgcctcc
      241 ttcaaagccg ttccaaccgc aaactcgccc cacatgtgca tcgggaccaa gtaggagacg
      301 gagacccgtt caagcagatc gagagcccct aacccatacc gcttccccag gcgcggatcg
      361 acgggaacga acgcgacgtc gatcgccgag ggcagccgat tgatctcttt gtgatactcc
      421 cgctccatcc atgaggtata cgcttctccc tcctcctccc agaaccagtt gttcagatcg
      481 ccggcgaagt agatgacctt tccttccacc tcaagcagga aggcgacccc ctcgtcggtg
      541 gattgaagag tccgtgcgac gaggccttca acctcgatcc gctgatggga acccatccga
      601 tggacgacga ttccatgggg aatatcttcg gtgggaatat cttcgctgag gatgaacgct
      661 ttggcgggaa ggtcgaagat ctttttattg taatgatcgc cgtgccggtg ggaagcgaag
      721 acgatgaggt ccttgttttc cggaagcgtc acctctccat cgaaatagtc gaagaggagg
      781 acggcctccc cgcattcgac gagaaaggaa gcgtgctgaa gaaacgtgac aaccatgcct
      841 acacgatgaa agagaagaaa gaaggtgtca agcgcctaca tcccctccag catcgtcagc
      901 gccgtctcaa tcagacccag ttcattgtaa tagtcgagaa tccccaaatg agggctccgg
      961 agcatcgtcc tgaggagctc gtccttaagt tcgccaagcg aacaccgccc ttcctcgacc
     1021 gaggtgatgc acccggcccc tttcagcttc ctcttgatct ccccgaaggt gaggatattc
     1081 gcttcaaggg tctgtttcac ctgattcagg gaggggcgcc tcctaaagga cttcggcttc
     1141 ttcgcaagga gcgcttcctg ctcggcaggg agaaagaggc gtttgatcag ttcttcccga
     1201 tcttttttca tgtgcccgac cgcatcccgt tcactgagcg tgaacagctg ctcgccgaga
     1261 tggaagatcc ccagagccaa gagggaaacg ggaactctgc ggttccgcct cgtgacatac
     1321 tcaagctcct gatcaaccac cccgccctga gccagcgctt tcgcaagaag ctcaaggctg
     1381 tacctgctgt tgtccaggta cgcttcgaag aactcgtcgg cgcttcccca tgccagagta
     1441 tgcagatcga tcctcaggtt ctcctcgccg aacagcaccg cctcgccgtg ggttgcaaga
     1501 agcagcaagg cctcggcgac cgcttgaggc aggatcttcg gctttctgat cagctcggtg
     1561 tcgacgagaa ggagcgtcgg aggctgggag ggaatgaacg cccccggcca ggtgaagcgc
     1621 cagggactgg cgatcgccgg tgaatcgaga accgtgggaa tcgcggcgaa ggagcgattg
     1681 agcttgtaag agacccgctt ccccactgaa aggaggcggt catccccgag aaagacgggc
     1741 accaccggtt tatcatccaa tagaggaaat atcttgttga acgcggctcg cttgaacgta
     1801 tgaccggttg cctccacccc ctctttgtga agggagcgga gaatggggag ggcgtagtgg
     1861 agatgttcat cctcttcagc gatgaccatc gcctgagcgt gcccgaaaca ctcgatatat
     1921 ccttcggcaa acttctgaag ggcgttttta ttaatgatga tgacctttgt ttccatgaga
     1981 catcgcctcc ttcttcatca agaggtgcgt tttttcaaga gcgttcgcac tgctcatccg
     2041 ggcaagaatc ttcttcatca aggcgaacag cccgccttgc ggggtgtagt ccgcttcgat
     2101 gaagaaattg atccgcccgg cggccatcag gcggtggcag aggtcgacct cgcctttggc
     2161 gtggacggcg atcgaaatac ctccggattc cttcaccagc ttcatgcacg gtacgtcggt
     2221 gagtccgtcc ccgatgtaga tcatgttggc aaagggaacc gggcgcagct cctgcggtgt
     2281 ataatcgttg agcgcattgt cctcccacac gtcaagaatc cctttgttga ttcgaaagag
     2341 aaattgcgtc tttgcggtat agttgatcgc cagcttcggc cacttggcga ccccgttcac
     2401 gtcgtagtag tactcgcatg cgtatatctt tttaaagcgg ctgccgatcg ccgaaccttc
     2461 gatgatctcg gtaagaccgc ttgagatgat gaaatgctcg atccggagtc ccagttccct
     2521 tccaagctcg ttgacatggt cgaaccacgc ttcgtcc
//
LOCUS       DAOPUZ010000163         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_42977_length_2540_cov_2.498189, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000163 DAOPUZ010000000
VERSION     DAOPUZ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42977_length_2540_cov_2.498189"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PKN79_03480"
     CDS             complement(<1..871)
                     /locus_tag="PKN79_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNZ94856.1"
                     /translation="MELKRFADVVAYLEGFLNLEKKTQHYTTREYRLDRMVALLDHVG
                     NPQESFQTIHLAGSKGKGSTASFIASGLTAMGYKTGLYMSPHLVDYRERFTLSGTYFS
                     DEALVRAGNLLKTSVGNFTFCDEWGETTPTTFELLTTYAYLLFKEEKCEWAVVETGLG
                     GRLDATNTLQSSASVLCPIELEHTRILGSTIGEIASEKAKIIKAGQPVFIGLVPEEAK
                     EVFLKECRKQRAKPFLLEELLAGYETRTTLEGERVTLAWSDGTEDRLLLSMKGSIQAQ
                     NVALALLALRTLGL"
     gene            1016..>2540
                     /locus_tag="PKN79_03485"
     CDS             1016..>2540
                     /locus_tag="PKN79_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ94857.1"
                     /translation="MSKKKHHAKPWDFLDEYRGKEFTGTWPTVVEMFDITVKRYPDNE
                     SFRAFTPKKEVYTYAEAYEHIQTIASYLVVEGVTKGTKVALTGKNSPQWAMSYLGILY
                     AGGTVVPLDASLSDEDMIKLMAFAGVKIFIADSERLKGFDEKDTLGLTHRLSLEPSEE
                     FPMIMEQKGDRRRKAYKATGEETAAILFTSGTTGTPKGVMLSHENLVSDCYLAQGNMW
                     IYPTDVFYAILPLHHSYTMMAVFIEAISVGASIVFGKKLVISQVLKEMREGKVTMFLA
                     VPMLFNKMIAALMNGVKEKGAVLYGVIRFLMGVSGVLKKVFKVNIGKKMFGFLLKKLS
                     LEHNRICISGGGPLPASTFKLFNQLGIDFVQGYGLTETSPITHLNPVEAYIETSVGKN
                     LPQVEVRIVDPDSDGNGLIHIKGPMVMQGYYKNPEATDEVLKDGWLNTGDVGYQDANG
                     YLYLTGRAKNLIVTEGGKNVFPEEIEDHFQLYDEIDTICVLGYIIDKQKKSEGIRALI
                     "
BASE COUNT          610 a          681 c          700 g          549 t
ORIGIN      
        1 agaggccaag ggtcctcagc gccagaaggg caagagcgac gttctgggct tggatcgaac
       61 ccttcatcga aagcaggagt cgatcttccg ttccatcgct ccacgcaagc gtcacccgtt
      121 ctccttccaa cgtcgtgcgg gtctcatatc cggcaaggag ctcttccagc aaaaaaggct
      181 tggcgcgctg cttgcggcac tccttgagga acacttcttt cgcttcttcg gggacgagcc
      241 cgatgaacac cggttgaccg gctttgatga tcttcgcctt ttcagaggcg atctccccga
      301 tcgtcgagcc gaggattctg gtgtgctcca gttcgatcgg acagaggacg cttgcgctgc
      361 tttgaagggt gttcgtcgca tcgagccggc cgccaaggcc ggtttcaacg accgcccact
      421 cgcacttctc ctctttgaac aggaggtatg cataggtcgt caacagctcg aaggtcgtgg
      481 gggtggtctc gccccattcg tcacagaagg taaaattccc gaccgacgtt ttcagaaggt
      541 tgccggcacg caccaacgcc tcatcgctga agtacgtgcc gcttaaggtg aaacgctccc
      601 ggtagtcgac gaggtggggg gacatataca gcccggtttt ataccccatc gcggtcaaac
      661 cgcttgcgat gaacgaggcg gtcgaaccct ttcctttgct tccggcaagg tggatcgttt
      721 gaaaggattc ttggggattg cccacgtggt ccaggagagc caccatccga tcaagccggt
      781 attcacgggt cgtgtagtgc tgagtctttt tctcgaggtt caaaaaccct tccaagtacg
      841 caaccacgtc ggcaaagcgt ttcagttcca tgaccgatac tgtagacact tgccggtttt
      901 tggtcaacga atctcattcg ttacactatt acgcaatgtg ataaggtgtt aatctgttgc
      961 caaaaagcgg gtttttctct tatagtgtta gaacccaaac agaggagggg aattcatgtc
     1021 caaaaagaag catcacgcaa aaccgtggga ttttcttgat gagtacaggg ggaaggaatt
     1081 taccggaacc tggccgaccg tcgtcgagat gtttgacatt accgtcaaac gataccctga
     1141 caatgaaagt ttcagagcat tcaccccgaa gaaagaggta tacacctacg ccgaggcgta
     1201 tgagcatatt caaacgatcg cttcctatct cgtggttgaa ggggtaacca aagggacgaa
     1261 ggtcgccctc accggaaaga acagccccca atgggcgatg agctatcttg gaatcctcta
     1321 tgccggtggc accgtggttc cccttgatgc aagcctctcc gatgaagaca tgatcaaact
     1381 gatggcgttt gccggagtga agatcttcat cgccgacagc gagcggctca aagggttcga
     1441 cgaaaaagac accctcggtc tcacccaccg cctcagcctt gagccgagtg aagagtttcc
     1501 gatgattatg gaacagaagg gggaccgccg taggaaagca tacaaggcga ccggcgaaga
     1561 gaccgcggct attcttttta ccagcgggac caccggaacc cccaaaggcg tcatgctcag
     1621 tcatgagaac cttgtcagcg actgctacct cgcccaaggg aacatgtgga tctatcccac
     1681 cgacgtattc tacgcgatcc tgccgctgca ccactcctac acgatgatgg cggtgttcat
     1741 tgaggcgatc agcgtcggcg cttcgatcgt cttcgggaaa aaattggtca tcagccaggt
     1801 attgaaggag atgcgcgaag gaaaggtcac gatgttcctc gccgttccga tgcttttcaa
     1861 caagatgatc gcagctctga tgaacggtgt gaaggagaag ggagccgtcc tctacggagt
     1921 aatccgcttc ctgatgggtg taagcggcgt gttgaagaag gtcttcaagg tgaacatcgg
     1981 aaagaagatg ttcggcttcc tcttgaagaa gctctctctg gaacacaacc ggatctgcat
     2041 cagcggcggc ggaccgcttc cggcctccac cttcaagctg ttcaatcagt tgggaatcga
     2101 ttttgttcag gggtacggtc tgacggaaac cagcccgatt acccacctca atccggtcga
     2161 agcgtatatc gagacctccg tcggaaagaa cctcccgcaa gtcgaggtgc ggatcgtcga
     2221 tccggacagc gacgggaacg gtctcatcca tatcaaggga ccgatggtga tgcaggggta
     2281 ctacaagaat ccggaggcga ccgacgaggt gctgaaggat ggctggctca ataccggcga
     2341 tgtcgggtat caggatgcca acggctatct ctacctgacc ggacgggcga aaaacctgat
     2401 cgtcaccgaa ggcggcaaaa acgtcttccc cgaagagatt gaggaccact tccagcttta
     2461 tgatgagatt gacacgatct gcgtcctcgg ctacatcatc gataagcaga aaaagagcga
     2521 agggattcga gccctcatct
//
LOCUS       DAOPUZ010000164         2535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_43087_length_2535_cov_3.325806, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000164 DAOPUZ010000000
VERSION     DAOPUZ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43087_length_2535_cov_3.325806"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PKN79_03490"
     CDS             <1..1002
                     /locus_tag="PKN79_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HNZ94858.1"
                     /translation="VATDSSGHEHHGTMVGTSTGIEPYYFWEWERTGRMGTNIERVKV
                     YDDWVKANPGKEKPDYFVSAMDLAPEGHVYVQAAIQRWVDSSISKTGNTPKEYTVEQT
                     GKLYELLYDTGCKGGTTYRDGSRDTQVLTIKKEEKEEKKETVLSFADPKPRVRATVLR
                     GTTYRKATPIGTAYITVNCDGPDPSDIFEVFINVAKVGSDVAADAEGLGRLISLILRM
                     PSPLTPEQRARAIITQLQGIGSGRSMGFGKNRVMSLPDAVGQVLEEHLGSTPSERPPM
                     DLPDEEDEEEGEGQLFLSLPTSKPGSVQADICPVCGNGTFMNIEGCRKCFSCGHSEC"
     gene            complement(1088..1330)
                     /locus_tag="PKN79_03495"
     CDS             complement(1088..1330)
                     /locus_tag="PKN79_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94859.1"
                     /translation="MTAPLRRRLYTVNHFRAIDYRIDMAEEPLSEEIIKHLHYLLKSR
                     TKNKTLSWLIPATMVGTRSVPRFSISILMFSAFDEH"
     gene            1591..>2535
                     /locus_tag="PKN79_03500"
     CDS             1591..>2535
                     /locus_tag="PKN79_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNZ94860.1"
                     /translation="MSNKRKVTIDGNTAAAHVAYAFSEVAAIYPITPSSTMGEFADEW
                     ASVGRKNMWGKTVEVVEMQSEAGAAGAVHGSLAAGALTTTFTASQGLLLMIPNMHKIA
                     GEMIPTVFHVSARSLAAQSLSIFGDHSDVMSCRNTGFAMTCANNVQEVLDLGLVAHLS
                     TLEAQVPFLAFFDGFRTSHEVQKVDELTYEDMYTLVDEKYVRRFRDRAMRPEKPVLRV
                     AAQNEDVYFQGRETVNKYYEALPEIVQKYMDKVAKLTGRTYHLFDYVGASDATDVVVI
                     MGSGADTVEWASDYINERGGSTGVLKVRLYRPFSAKHFV"
BASE COUNT          571 a          685 c          735 g          544 t
ORIGIN      
        1 gttgccacgg actcgagtgg gcatgagcac cacgggacga tggtcggcac ctcgacgggg
       61 atcgagccct actacttctg ggagtgggaa cggaccggcc ggatgggaac caatatcgag
      121 cgggtcaagg tgtacgatga ctgggtcaag gcgaaccccg gcaaggaaaa acccgattac
      181 ttcgtcagcg cgatggatct cgctccggag gggcacgtct atgtccaagc ggcgattcag
      241 cgctgggtcg actcttcaat ctccaagacg gggaacactc ccaaggagta caccgtcgag
      301 cagaccggca agctctatga gcttctctat gacacggggt gcaagggagg taccacctat
      361 cgcgacggct cccgggatac tcaggtcctg acgatcaaga aggaggagaa ggaagagaag
      421 aaagagacgg tgctgagctt cgccgatccg aagccgcggg ttcgggcgac ggtccttcgg
      481 ggaaccacct accgcaaggc gacgccgatc ggaacggcgt acatcaccgt caactgcgac
      541 ggtcccgatc cgagcgatat cttcgaagtg ttcatcaacg tcgccaaagt cggctccgac
      601 gtcgccgccg acgccgaagg cctcgggcgc ctgatcagcc tcatccttcg aatgccctcg
      661 ccgctgacgc ccgagcagcg ggcccgggcg atcatcaccc agctacaggg aatcggcagc
      721 ggccgttcga tgggcttcgg aaaaaatcgg gtgatgagtc ttcccgatgc ggtgggccaa
      781 gtgcttgagg agcacctcgg ctccaccccg agtgaacgtc ctccgatgga tcttcccgat
      841 gaagaggatg aagaggaggg cgaaggccag ctctttttga gtttgccgac ttccaagccg
      901 ggctcggttc aggcggatat ctgcccggta tgcggcaacg gaacgttcat gaacatcgaa
      961 ggatgccgaa agtgcttctc ctgcgggcac agcgagtgtt gagaagatat caagaaggga
     1021 tgggataggg ggtccgctca ggcgggcctc ctatccgtca tgtcgaagct ttacgacgat
     1081 agcacaatca atgttcatca aaagcggaaa acatcaatat tgaaatactg aaccggggca
     1141 cggaacgcgt cccgaccatc gtagcaggta tcaaccagga aagagttttg ttctttgtcc
     1201 tgcttttaag gagatagtgc agatgcttga tgatctcttc actgagcggt tcttccgcca
     1261 tgtcaatgcg gtagtcaatg gcacggaagt gattgaccgt atagaggcgg cgtctcaacg
     1321 gagccgtcat attgttatga tcgcaagatc aatgacatcc ctaattccta ctatttctct
     1381 ggtgattttc ctgtttgttg ttgatgtaaa gagttagcat cttgaaactt tatttcttta
     1441 ctccgaatag aatattttcc gtgttctctt gcaaacgaac ccttattggt ctatgattgg
     1501 ctactgggaa aaatctgcga tcttcccaca tttttgtgct tagggcagcc ccttccgggt
     1561 tgcagcaaat actgctttat ggagagtcac atgagtaaca aaaggaaggt taccatcgac
     1621 ggaaacaccg ctgctgcgca cgtagcatac gcgtttagcg aggttgccgc catctatccg
     1681 attacgccct cttccacaat gggtgagttt gccgacgaat gggcaagcgt tgggcggaaa
     1741 aatatgtggg gaaaaacggt cgaagtcgtc gagatgcagt cggaagccgg tgccgccggc
     1801 gcagttcacg gttcgctcgc ggcgggtgct ttgacaacca cgtttaccgc cagtcaggga
     1861 ttgttgttga tgattccgaa catgcacaag atcgccgggg agatgatccc gaccgttttc
     1921 cacgtttcgg cccgatctct tgccgcccaa tccctttcga tcttcggtga tcattccgac
     1981 gtgatgagct gccgaaacac cgggttcgcg atgacgtgcg cgaacaacgt ccaggaggtc
     2041 ttggacctcg ggctggtcgc tcacctttca acgctcgagg ctcaggttcc tttccttgcg
     2101 ttctttgacg gcttccgcac ctcccacgaa gtgcagaaag tcgatgagct tacctacgag
     2161 gacatgtaca ccctcgtcga tgagaaatat gtccgccgct tccgcgatcg tgcgatgcgc
     2221 ccggagaagc cggttctccg cgtagccgct cagaacgaag acgtatactt ccaaggtcgg
     2281 gagacggtga acaaatatta cgaagccctg ccggagatcg tccagaagta catggacaag
     2341 gtcgccaagc ttaccggccg aacctatcat ctcttcgact acgtcggcgc ttccgatgcg
     2401 acggatgtgg ttgtcatcat gggaagcggt gccgacacgg tggaatgggc gagcgactac
     2461 atcaacgagc gcggcgggtc gaccggtgtg ttgaaggtcc gcctctatcg ccccttcagc
     2521 gccaagcact tcgtt
//
LOCUS       DAOPUZ010000165         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_43175_length_2531_cov_3.896607, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000165 DAOPUZ010000000
VERSION     DAOPUZ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43175_length_2531_cov_3.896607"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PKN79_03505"
     CDS             complement(<1..438)
                     /locus_tag="PKN79_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNZ94861.1"
                     /translation="MYHHLFLDLDGTLMDFREAERRAFTRMAGQLRIPIDDASHLLYA
                     RCNHSCWKEFEQGLLTIDELSLKRFALFQEQSNLRFDVESASEVYEDHLSRQAILYPE
                     TLPLLTLLKDEGYRLHLASNGISRVQRGRLEASNLERFFDHIFI"
     gene            513..1100
                     /locus_tag="PKN79_03510"
     CDS             513..1100
                     /locus_tag="PKN79_03510"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNZ94862.1"
                     /translation="MKKRHWALGAIVGVVLLALGIFLIFQEESFKKIFVSLLGVYMIG
                     SGFSTLLGLKSYQLGPRLRPATLIKALLTLLLGVIALILPILVADLSFLILLYIIATE
                     LLFSGIISIINLVAVRNLDIAFSPIIGDALISLILSMLLFFFPRQIGTVLLKGVGILV
                     IAIGLFFIIASLITRRTGRREEGKTIEGEAEILES"
     gene            complement(1097..1375)
                     /locus_tag="PKN79_03515"
     CDS             complement(1097..1375)
                     /locus_tag="PKN79_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94863.1"
                     /translation="MRSCSHCSTTIDSTLSIGYATLCPTCSEPLHSCVNCRFYSPGSY
                     HDCAEHVEEFISDKNKANYCDSFVLGCNHPTKKASDEARKKAEAIFSF"
     gene            complement(1375..1779)
                     /locus_tag="PKN79_03520"
     CDS             complement(1375..1779)
                     /locus_tag="PKN79_03520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNZ94864.1"
                     /translation="METVFTKIRAGAIPSAKVYEDEICFVILDINPVNKGHLLIITNE
                     VHPTLADTPDEVLSHLLVIAKKADAKLREVLGCAATNVVINNGEASGQEIPHLHLHII
                     PRYTDDGKRLTFPKTHYDEGEIAALGKRLEFE"
     gene            1965..>2531
                     /locus_tag="PKN79_03525"
     CDS             1965..>2531
                     /locus_tag="PKN79_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ94865.1"
                     /translation="MTDLRHLRNIGISAHIDSGKTTLSERILYYTNKIHEVHEVRGKD
                     GVGATMDSMELERERGITIASAATNVVWKGNEINVIDTPGHVDFTIEVERSLRVLDGA
                     ILVLCSVGGVQSQSITVDRQMKRYKVPRIAFVNKCDRTGANPYKVKNQLIEKLGLNAL
                     LMQIPIGLEDRLEGVVDLVAMKALYFDAG"
BASE COUNT          551 a          659 c          733 g          588 t
ORIGIN      
        1 gatgaagatg tggtcgaaga agcgctcaag gttcgaggct tccagtcgtc cccgttggac
       61 tcggctgatt ccattggatg caaggtggag gcggtatccc tcatccttca aaagggtgag
      121 aagggggagg gtttcagggt agagaatcgc ttggcggctg aggtggtctt cgtacacctc
      181 gcttgccgat tccacatcaa agcgaaggtt gctctgttct tggaagaggg caaaccgctt
      241 caaggagagc tcatcgatcg tcaacagccc ctgttcgaac tccttccagc aggaatggtt
      301 gcaccgggca tagagcaggt gcgaggcgtc atcaatggga atccgcaatt gcccggccat
      361 ccgggtgaat gcccgcctct cggcttccct gaaatccatc aatgtcccgt cgaggtcaag
      421 aaaaagatgg tggtacatgg cctaactcct ttacttgcag attgctaaga agtaccatac
      481 acatcaggaa caccttgtac aaggagaacg tgatgaagaa acgtcattgg gccctcgggg
      541 cgatcgtcgg agtggtattg ttggccttgg gcattttcct gatctttcag gaggagtcgt
      601 tcaagaaaat ctttgtctcc ttgctcgggg tatatatgat cggttcgggg ttttccaccc
      661 tgctggggct caaatcctat cagctcggtc cccggctcag gcctgctacc cttatcaagg
      721 cgctgctcac ccttctgttg ggggtgatcg ccctgatcct gccgattctc gtcgccgacc
      781 tcagcttcct gatccttctg tacatcatcg ctacggagct tctcttcagc ggaatcatct
      841 cgatcatcaa ccttgtagcg gtccgaaatc tcgacatcgc tttttctccc ataatcggtg
      901 atgccctgat cagcctaatt ctctcgatgc tgctcttctt ctttcccaga caaatcggaa
      961 cggtgctgtt gaaaggggtg gggattctgg tgatcgcaat cggacttttc ttcatcatcg
     1021 cttcgctcat cacccgacgg acggggaggc gggaagaagg aaaaacgatt gagggagaag
     1081 ccgagattct cgagtcttag aaggaaaaga tcgcttccgc tttttttcgg gcctcatcac
     1141 tcgctttctt cgtcgggtgg ttgcaaccca ggacgaagga atcgcagtag ttcgccttgt
     1201 tcttgtcgct gatgaactct tcgacgtgct cggcgcagtc atggtaggac ccgggggagt
     1261 agaaacggca gttgacgcag ctgtgcaggg gctccgagca ggtcggacac agggtagcgt
     1321 atccgatcga aagcgtggaa tcgatcgtcg tcgaacagtg ggaacagctg cgcatcactc
     1381 gaactccaag cgcttgccca atgctgcgat ctccccctcg tcatagtgcg ttttgggaaa
     1441 ggtcaggcgt ttgccgtcat cggtgtagcg ggggatgatg tgcagatgga ggtgggggat
     1501 ctcctgtccg ctcgcctcgc cgttgttgat caccacgttg gtggcggcgc agcccagcac
     1561 ttcccgaagc ttggcgtcgg ctttttttgc gatcacgagc aggtgggaca acacctcgtc
     1621 gggggtatcg gccaaggtgg gatggacttc gttggtgatg atcaagaggt gccccttgtt
     1681 caccgggttg atgtcgagaa tcacgaagca gatctcgtcc tcatacacct ttgcggacgg
     1741 aatggctccg gctcgaattt ttgtaaaaac agtctccatg gcacacctca aaccaagtat
     1801 accacagctg tagcagggat tgaacaaaaa agagagatgg tgtagtatct gctggtgttt
     1861 tggacccatc tccttatagg gttgaactct acgacatcgg cctctttcgg ccaccgacac
     1921 atactgttga actatcattc gaagaatata gaggtaatgg aagcatgact gacttgcgac
     1981 acttgaggaa tattgggatc agcgcccata tcgactcggg gaagacgacc ctttccgagc
     2041 gaatcctcta ctatacaaat aaaatccacg aagtccatga agtccgcggc aaggatggcg
     2101 tcggcgccac gatggacagc atggagctgg agcgggagcg gggcatcaca atcgcaagcg
     2161 ccgcgacgaa cgtcgtgtgg aagggaaatg agatcaacgt gatcgatact cccggccacg
     2221 tcgactttac gatcgaggtc gagcgctccc tgcgggttct cgacggcgcg attctcgttc
     2281 tgtgctccgt cggaggagtg cagagccagt cgatcaccgt cgaccggcag atgaagcggt
     2341 acaaagtccc ccggatcgcc ttcgtgaaca agtgcgaccg caccggggcc aacccgtaca
     2401 aagtgaagaa ccagctcatc gagaagctgg ggctgaacgc cctcctgatg cagattccga
     2461 tcggtctgga ggatcgcctg gaaggggtgg tggaccttgt cgcgatgaag gcgctctatt
     2521 tcgatgccgg t
//
LOCUS       DAOPUZ010000166         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_43201_length_2530_cov_3.755960, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000166 DAOPUZ010000000
VERSION     DAOPUZ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43201_length_2530_cov_3.755960"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKN79_03530"
     CDS             complement(<1..310)
                     /locus_tag="PKN79_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HNZ94866.1"
                     /translation="MDMFKRNLAPLSNRAWSEIEERARLVLLSRLSARKVVSVNGPHG
                     LDYTVLSEGRLSLVDDKSTVKAGIYNVLPLTEARVRFTLNKWELDNLERGAKDIDFDS
                     L"
     gene            complement(323..679)
                     /locus_tag="PKN79_03535"
     CDS             complement(323..679)
                     /locus_tag="PKN79_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNZ94867.1"
                     /translation="MPYTEPYDVLDEKTRDISRAITSLKEEIEAVNWYNQRVYTTKDP
                     ELAAIMAHNRDEEIEHACMTIEWLRRNMDKWDEELRTYLFTDGSIIEAEESAMGKTGS
                     SAAKSGSSLSIGNLKK"
     gene            873..>2530
                     /locus_tag="PKN79_03540"
     CDS             873..>2530
                     /locus_tag="PKN79_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ94868.1"
                     /translation="MERTIVSLLQSAAEKYQDRAYTNTRFDQGWVPSSFKQTNLDSDY
                     VAAFLLEDGVKSQMCVGILSEGKGQWVTFEHGALKARMVSVPLSIKLTAEEVAFRINH
                     SEAVMLAVSSNTLALVAAAFPSFDHPVKLVYLDAPDERLAKHVEEKKWKEGKDYVVWP
                     EILKKGEAILKKKPKLVSDSIAKIKEDDTINICYTSGTTGNPKGIMLTHLNYWANVQG
                     AVELFKIPDGTFETLIILPVDHSFAHTVGIYTSMVCGITLHFVDSRGSNAAIIRNFPK
                     NLVELNPTFLMTVPSITGSFMKKMTQGIAAKGPFIEGIFKRGLEAGIARNGDGFNKGS
                     KFKGWFHYHLANLLVFPKLRKVFGNKFLYCVGGGALLEARQQHFFAAIGSPVYQGYGL
                     TEAAPIICSNSPGRYKFGSSGMVLHNIECRIMVDDETEAKVGQRGEIVIRGDNVMKGY
                     FKNPEATAEALRDGWLWTGDLGYYDEDGFLVVTGRAKALLIGKDGEKYSPEEVEEVMV
                     NNVASINQLMVYNDHQAITTALVTLQEDIVKRMIKEKGLSTPEAT"
BASE COUNT          613 a          650 c          678 g          589 t
ORIGIN      
        1 ccaagctgtc gaagtcgatg tctttcgccc cgcgctccag gttgtccagt tcccacttgt
       61 tcaaggtgaa gcggactcgg gcttcagtca gcggcagcac gttgtagatt cccgccttta
      121 ctgtgctctt gtcatcaacg agggagagcc ggccttcgct gagcaccgta taatcgagtc
      181 catgaggccc gttgacggaa acgaccttgc gggcgctcag gcgcgagagc aaaacgagcc
      241 ttgcccgctc ctcgatctcg ctccatgccc ggttggagag gggagcgagg tttcgtttga
      301 acatatccat agtattcctt ccttatttct tcagatttcc aatggaaagc gatgagccgc
      361 tcttcgcagc ggagctgccc gtttttccca tggcgctttc ttccgcttca atgatcgacc
      421 cgtcggtgaa gaggtaggtt ctgagctctt cgtcccactt gtccatgttt cttcgaagcc
      481 actcgatcgt catgcaggca tgttcgatct cttcgtcccg gttgtgcgcc atgatggcgg
      541 caagttcggg atcctttgtc gtatagaccc gctggttata ccagttaact gcttcaattt
      601 cttctttcaa actggtgatt gcccgcgaaa tatcacgagt cttttcatcc aatacgtcat
      661 acggttcagt atatggcata cattcctcct gtttttatgt gttactgata cagtgtacta
      721 ctagaagggg ttcaatggaa gtggtacaga gtcgtttcct tcaccctcgg tggatgtata
      781 ggtcaaatga attaaagaga agaacatctt gtttttttcg acaataatta taataatgta
      841 tatattaaaa acaaacaggt tgagagagca ctatggaacg aacaattgtc tcccttttgc
      901 agagcgcagc ggagaaatac caagaccgtg cgtacacaaa cacccgcttt gatcaaggct
      961 gggttccctc ctccttcaag cagacgaatc tcgacagtga ttacgttgcg gcgttccttc
     1021 tcgaggacgg cgtcaaaagc caaatgtgcg tgggaattct cagtgaagga aaagggcaat
     1081 gggtaacctt tgagcatggg gcgctgaaag cccggatggt cagtgttccc ctttcgatca
     1141 agcttaccgc tgaagaagtg gcgtttcgga tcaaccacag cgaagcggtg atgctcgcag
     1201 tctcttccaa cacgctggcg cttgttgcgg ccgcattccc ttcattcgac catccggtga
     1261 agttggtcta tcttgacgcc ccggacgagc ggcttgcgaa gcacgtggaa gaaaagaagt
     1321 ggaaggaagg gaaggactat gtcgtctggc ctgaaattct caaaaaaggc gaagccattc
     1381 tgaaaaagaa gccgaagctg gtcagtgact cgatcgcgaa gatcaaggaa gatgatacga
     1441 tcaatatctg ttataccagc ggaacgacgg gcaatccgaa agggatcatg ctgacccacc
     1501 tcaactattg ggcaaacgtc cagggagcgg tcgagctgtt caagattccc gacggaacgt
     1561 tcgagaccct gatcatcctg cccgtcgatc acagtttcgc ccatacggtg ggaatctata
     1621 cctcgatggt ttgcggcatc acgctgcact tcgtcgattc acgaggttcc aatgccgcca
     1681 tcatccgaaa ttttccgaaa aacctggtcg agctgaatcc cactttcctg atgacggttc
     1741 cttcaatcac cggttcattc atgaagaaaa tgacccaggg gatcgccgcc aaggggccgt
     1801 ttattgaagg gatcttcaaa cgcggtcttg aggccggaat cgcccgcaac ggggacggct
     1861 tcaataaagg gagcaaattc aaaggctggt tccattatca cttggccaat cttttagtct
     1921 tccctaaact ccgaaaggtg ttcggaaaca agttcctcta ctgcgtcggc ggcggagcgc
     1981 tcttggaagc gcgccaacaa cacttcttcg cggcgatcgg ttctccggtg tatcagggct
     2041 atgggctgac ggaagccgcc ccgatcatct gctccaattc cccgggacgc tacaagttcg
     2101 gaagcagcgg aatggttttg cacaacatcg agtgccggat catggtcgat gacgagaccg
     2161 aggccaaggt agggcagcgg ggcgagatcg tcattcgcgg cgacaacgtg atgaagggat
     2221 acttcaagaa ccccgaagcg accgccgagg cgcttaggga cggctggctt tggaccgggg
     2281 atctgggcta ctacgacgaa gacggcttcc tggtcgtaac cggccgggcg aaagcattgc
     2341 tcatcggcaa ggatggggaa aaatacagcc ccgaagaggt cgaagaggtg atggtaaaca
     2401 acgtcgcttc gatcaaccag ctgatggtgt acaacgacca ccaggcgatc acaacagcgc
     2461 tggtcaccct tcaggaagat atagtcaagc gaatgattaa ggaaaaagga ttgtcgacgc
     2521 ctgaagcaac
//
LOCUS       DAOPUZ010000167         2514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_43572_length_2514_cov_2.946726, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000167 DAOPUZ010000000
VERSION     DAOPUZ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43572_length_2514_cov_2.946726"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PKN79_03545"
     CDS             complement(<1..527)
                     /locus_tag="PKN79_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ94869.1"
                     /translation="MKELLTVDTVSVSYGNIRALKEVTMHVYEGDIVCLIGANGAGKS
                     TLLKAIVGLEPLVEGTITFDGEEICTANRGRHMTTDLIASRGIALVPEGRRVFADMTV
                     EENLDMGAFLVRDDALIAQRKEAMYDFFPILGARKRQKTRSLSGGEQQMMAIARALMS
                     GPRMILLDEPGLGLAP"
     gene            complement(527..1303)
                     /locus_tag="PKN79_03550"
     CDS             complement(527..1303)
                     /locus_tag="PKN79_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ94870.1"
                     /translation="MTILETERLTRAYGGVIAVNNVDFQVEGGLITGLIGPNGAGKTT
                     LFNNMTGLDTPSSGKVRFMGKDITGYPAHKICRLGIARTFQNIRLFKDLTVAENVMIG
                     RHFKTGKDETKGRFLNALKSYIHLKEEENAIYDKALQWLDFFDMHSYRSELARNLPYG
                     KQRELEIARALATDPQLLFLDEPAAGMNPQETEHLIRIIRKIRELGITVVLIEHDMRL
                     VMNICDSITVLNYGEKLAEGTPTEIKNNPLVIEAYLGKDE"
     gene            complement(1300..2187)
                     /locus_tag="PKN79_03555"
     CDS             complement(1300..2187)
                     /locus_tag="PKN79_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNZ94871.1"
                     /translation="MILIYSGIYALMALGQNVITGYGGMLSLTQAGFFAIGSYATAIL
                     STRFGFSFWATLPPAALLSALCGLLIGLPTLRLKGDYLAIATLGFGEIVRNVLNNWDS
                     LTRGPMGIQKIPMPTIFGFVINPYTKYSFLVMVVIFVAIAYVLFQRLARSRMGRALAA
                     IREDEIAAEAMGINITKYKVYAFILGASVAGIAGSLQAVFTLSVTPGTYTFMVSVMVL
                     CMVVLGGMGNFKASILGAFIIQLISYFPQLTGLTSVIPPQFKQILFGLILVVMMIWRP
                     QGLLGREEIRYGNEEGEKA"
     gene            complement(2207..>2514)
                     /locus_tag="PKN79_03560"
     CDS             complement(2207..>2514)
                     /locus_tag="PKN79_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNZ94872.1"
                     /translation="QFVGSALAAVGGILIALDFKVYATMGTMTGLKAFVAAVFGGIGN
                     ITGAMVGGILLGILETFGVAIFGIPSGLKDTIAFGVLIIILLVKPAGLLGKDEKEKV"
BASE COUNT          544 a          756 c          684 g          530 t
ORIGIN      
        1 ggcgccagac cgagtcccgg ctcatccaac agaatcatcc tcggtccgct catcaacgcc
       61 cgggcgatcg ccatcatctg ctgctcgcct ccggagagcg agcgggtctt ttgacgcttc
      121 cgcgctccga ggatcgggaa gaaatcatac atcgcctcct tgcgctgagc gatgagagca
      181 tcgtcgcgga caaggaacgc ccccatatcc aagttctctt ccaccgtcat gtcggcaaac
      241 acccgccgcc cctccggaac gagggctatt ccccgtgatg cgatcagatc ggtagtcatg
      301 tgacggcccc ggttggcggt gcagatttct tccccgtcga aggtgatcgt gccttcaacg
      361 aggggttcca gtccgacgat cgccttcaga agggtggatt ttcccgctcc gttggcaccg
      421 atcagacaga cgatgtctcc ctcataaacg tgcatcgtca cctctttgag ggcccgaata
      481 ttgccatagg aaacggaaac agtatcgacg gtaagcagtt ctttcatcac tcatcctttc
      541 caagatacgc ttcgatcacc aaaggattgt tcttgatctc cgtcggagta ccctcggcaa
      601 gcttctctcc atagttcaac acggtaatcg agtcgcagat attcatcacc agcctcatat
      661 cgtgttcgat caagacgacg gtgattccaa gctcccggat cttcctgatg attcgaatca
      721 aatgctcggt ttcctggggg ttcattcccg ccgccggctc atcgagaaag agcagctgag
      781 gatcggtggc gagcgccctt gcgatttcca gctcccgctg cttcccgtac ggcaggtttc
      841 tggccagctc gctccgatag gagtgcatat caaagaaatc aagccactga agcgctttgt
      901 cgtagatcgc gttctcttcc tctttcagat gaatgtagct tttcagagcg ttgagaaaac
      961 gcccttttgt ttcatctttg ccggttttga agtggcggcc gatcatcacg ttctccgcaa
     1021 cggtgagatc tttgaagaga cggatgttct ggaacgtccg ggcgatgccg aggcggcaga
     1081 tcttatgagc gggataaccg gtgatatcct tacccatgaa gcggaccttc ccgctcgaag
     1141 gagtgtccaa gccggtcata ttgttgaaca gcgtcgtttt tcccgctccg ttggggccga
     1201 tgagtccggt gatcaggccg ccttccactt ggaaatcaac gttgttgacc gcgatcactc
     1261 cgccgtaggc tcgagtcagt cgttccgttt ccaggatggt catgccttct ctccttcctc
     1321 gttgccgtag cggatttctt cccggccgag cagaccctga ggccgccaga tcatcatcac
     1381 caccaaaatc agtccgaaga ggatctgttt gaactgcggc gggatcacgc tggtcaggcc
     1441 ggtaagctgc gggaagtagc tgatcagctg gatgatgaac gctccgagaa tcgaagcctt
     1501 gaagtttccc atccccccga ggacgaccat gcacaggacc atcaccgaga ccatgaacgt
     1561 ataggtgccc ggggtgacgc tcaaggtgaa gaccgcctga agcgacccgg cgatgcccgc
     1621 gacggaagcc cccagaatga acgcatagac cttgtacttg gtgatgttga tccccatcgc
     1681 ttccgctgcg atctcgtctt cacggatcgc ggcgagcgcc cgccccattc gtgaacgggc
     1741 aagccgctgg aacagcacgt atgcgattgc aacgaagatg acgaccatca ccagaaacga
     1801 atatttggta tagggattga tcacgaaccc gaagatcgtc ggcatcggaa tcttctggat
     1861 tcccatcggt ccccgggtca gactgtccca gttgttcagg acgttgcgga cgatctcccc
     1921 gaagccgagg gtggcgatcg ccaaatagtc acctttgagc cggagggtcg gcagcccgat
     1981 caagaggccg caaagcgcgc tcaagagcgc cgcggggggc agcgtcgccc agaaggagaa
     2041 cccgaagcgg gtggaaagga tcgccgtggc gtaggagccg atcgcgaaga accccgcctg
     2101 cgtcagactg agcatccccc cgtaaccggt gatgacgttc tgcccgagcg ccatcaatgc
     2161 atagatgccg gaatagatca agatcaataa aaagaagttg agcatatcac actttctcct
     2221 tctcatcctt gccgaggaga cctgccggtt tgaccagaag gatgatgatc agcaccccga
     2281 aggcgatcgt gtccttcagc ccgctgggaa ttccgaagat cgcaaccccg aaggtctcaa
     2341 ggatcccgag caggatccct ccgaccatcg ctccggtgat gtttccgatc cccccgaaca
     2401 cggcggcgac gaacgccttc agcccggtca tcgttcccat cgtcgcataa accttgaaat
     2461 cgagggcgat gagaattcct ccgaccgcgg ccagggcgct gccgacgaac tggc
//
LOCUS       DAOPUZ010000168         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_43651_length_2511_cov_2.640472, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000168 DAOPUZ010000000
VERSION     DAOPUZ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43651_length_2511_cov_2.640472"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..985)
                     /locus_tag="PKN79_03565"
     CDS             complement(335..985)
                     /locus_tag="PKN79_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94873.1"
                     /translation="MTYIRKLIKVFLGIILYALGVVITIEARIGFSPWEVFHAGLANV
                     LNLQIGTISIGVGLILITITTIFGEKIGMATVLNIIFVGLTVNLILNAEIIPTSKRFS
                     FSIIQMTVGLFIMVFATYLYLSTGLGAGPRDAFMVLINRRTGIKVGIVRSLIEITVTI
                     LGALMGGLFGWGTIMAAVIIGIALQTVFRIFKFDPRTVEHEDIKTTLTSMLNRPAP"
     gene            complement(982..2202)
                     /locus_tag="PKN79_03570"
     CDS             complement(982..2202)
                     /locus_tag="PKN79_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ94874.1"
                     /translation="MYKSTIRACHLGSFIGALTVNLAPLLFVIFMTQFGLNFEQVGRL
                     VLLNFGIQIVTSLLFSSPVDRWGQKPFILFAHLMVFIGLILLALAPLLFTSIPYLGLA
                     FATIFYSVGAGLFELQLSAIIQSIPTDNKEGAMNLLHSFYAWGLIFVVVLTTTLLSVF
                     GKGSWPFIVLFWSIFPLYNFFNFLRVPFPPALQAQERTKTVNLFKSKYFLPVLAGIFF
                     GGATELSISQWTSAFAESSLGLRKEIGDLLGVTLFALFLGGARTLYGALGKKIDLLKA
                     MTMGSMLCVGMYLLAAFSPSPLISLIACVLCGLGSALLWPGSVVNGANHFPKAGSSLF
                     AALAAGGAAGAAFGPWLVGLIASIKPDLVKIAPWLDFLTTEEAALRSGLFIGAIFPII
                     MIILLTLMRRENST"
     gene            complement(2230..>2511)
                     /locus_tag="PKN79_03575"
     CDS             complement(2230..>2511)
                     /locus_tag="PKN79_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94875.1"
                     /translation="NYYQSIETFVHRNKVTGSDLLQAIAEKKEIKNISADTLQTITEW
                     LTKNGFLSEDGAYTKEEIISQLSVKHEDLRIGSDEQLIVERYLSHILDP"
BASE COUNT          672 a          639 c          663 g          537 t
ORIGIN      
        1 gtcctggtct atcgatacgg gtagcttcaa caacgaatca gcactctgcc tccaattttc
       61 agtggaagca atacagcgag cgaccttcac ttcagcaaag acggatcata cctctactgt
      121 tcgaatcggg ggcatgactc ggtggcgatt tttcgagtga accacagctt gcgccgtttc
      181 gaatactgca gtacagataa agaaccgaga agcttcgtcc tttcaccgga tggcttatgg
      241 atgctggtcg ccaatgcctc aagcaacacg attacgattc accgccgcaa ccaagtcaac
      301 ggttcggtcg gagaaaaaac ccatcggttg gccgtcaggg agccggtctg tttaacatgg
      361 atgtaagtgt cgttttgata tcttcatgtt caacggtccg aggatcgaat ttgaagattc
      421 ggaataccgt ctgaagagcg attccgataa tgacagccgc catgatcgtc ccccatccga
      481 acaggccgcc catcagggcg cctaagatcg ttacggtgat ttcaatcagg gagcgaacaa
      541 tgcctacttt gattccggtc cttcggttga tcagcaccat aaacgcgtcc cgcggccccg
      601 ctccaaggcc ggtagagagg tacaggtagg ttgcaaacac catgatgaag agcccaaccg
      661 tcatctgaat gattgaaaag gaaaaacgtt tggaagtcgg aatgatttcg gcgttgagaa
      721 taaggttcac ggtcaatccg acaaagataa tgttgagaac cgtcgccatt cctatttttt
      781 ctccaaagat ggtggtgatc gtgatcagaa tcaaccctac tccgatggag atcgttccta
      841 tttgaaggtt caatacgttg gcaaggccgg catgaaacac ctcccacgga gaaaacccga
      901 ttcgggcttc tatcgtgatt acaaccccta aagcgtagag aatgattccg agaaagacct
      961 tgatgagttt tctgatgtac gtcatgtact attctcgcgc ctcatcaggg taagcagaat
     1021 aatcatgatg atgggaaaga tcgctccaat gaacagtccg cttctcaagg ctgcttcctc
     1081 ggtggtgagg aaatccagcc agggagcgat cttcacgaga tccggtttga tgcttgcaat
     1141 cagtccgacc agccacggac cgaacgccgc tcccgccgct ccaccggcgg caagcgcagc
     1201 aaacagggaa gatccggctt tgggaaaatg attcgctcca ttgaccaccg agccgggcca
     1261 aagaagggcg ctgccaagac cacagaggac gcaggcgatc aagctgatga gtggagaggg
     1321 actgaatgcg gcaagcaggt acatcccaac acagagcatg gagcccatcg tcatcgcttt
     1381 cagaagatcg attttttttc ccaacgctcc gtagagagtg cgggctcctc caagaaagag
     1441 ggcgaaaagg gttaccccaa gcagatctcc gatctctttt ctcaatccca gcgacgattc
     1501 ggcaaatgcg ctcgtccact gggagattga aagttccgtc gccccaccga agaaaattcc
     1561 cgcaagtacg ggcagaaagt actttgattt gaaaaggttg accgttttcg tccgctcttg
     1621 ggcttgaagc gccggaggaa acgggacccg aagaaaattg aaaaaattgt agagggggaa
     1681 gatcgaccag aacagcacga tgaagggcca tgaacccttt ccgaagacgg aaagaagcgt
     1741 cgtggtgagc accaccacga agataagacc ccaagcgtag aaggagtgca gcaggttcat
     1801 cgctccctcc ttgttgtccg tcgggatcga ttggatgatc gcgctgagct gcaactcgaa
     1861 aagacccgct cccaccgaat aaaagatcgt cgcgaatgcc aagccgaggt aaggaatcga
     1921 ggtgaaaagc agcggggcaa gagccagaag aatcaatccg atgaagacca tcaggtgggc
     1981 aaaaaggatg aacggctttt gtccccatcg atcaaccggg gaactgaaga gcaggcttgt
     2041 gacgatctgg atgccgaagt tcaggagcac caagcgaccg acctgttcga agttcaagcc
     2101 gaactgcgtc atgaagatga cgaacaacag cggtgcgaga ttgaccgtca acgcaccgat
     2161 gaaactgccg agatggcatg ccctgatcgt ggatttgtac atagctccga gtatatgcga
     2221 taacgggcgc taagggtcaa ggatgtggga aagatatcgc tctacgatga gctgttcatc
     2281 acttccgatc ctcaaatcct catgcttgac gctgagctgc gagatgatct cctccttcgt
     2341 atacgcccca tcctcgctga ggaaaccatt cttcgtcagc cattcggtga tcgtctgcaa
     2401 ggtatcggct gagatgttct tgatctcctt cttctcggcg atcgcctgaa gcaagtcgga
     2461 gccggtaacc ttgttccgat gtacaaaggt ttcgatgctc tgatagtaat t
//
LOCUS       DAOPUZ010000169         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_43756_length_2507_cov_2.924959, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000169 DAOPUZ010000000
VERSION     DAOPUZ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43756_length_2507_cov_2.924959"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..2229
                     /locus_tag="PKN79_03580"
     CDS             196..2229
                     /locus_tag="PKN79_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94876.1"
                     /translation="MKRLETFILVILLTLIAPLSIFGQEYVSVIEGDLASLRLYEPNG
                     KPISIETIDINDLQLGTILVSGDEPIILETSRGDIRLFPDSILILDEVSATSTTLLLI
                     DGSVTAKTTQGTLSIITPATLYELDRAGEVFVTSTRNEERVQSFDVAVRATNLITHKT
                     TTIEALQELELSNPKLAKRTLAPAEAAAGRIVRPARIPAQPGAIQWTVGLEFVEEAEE
                     IKELVRSLPAKPVVLSPSVKAMPVVKEAVAKPRTTLRDSAPAPIQSKGTYGIEAGYTL
                     TFPIKSASYWPGHRLEVKPFISYNSLALRLNVHIETDSFTAFDTNFLNLDPSALGIVS
                     FVFGIVDHVKVGYSTSPFYLLIEEGGYPGTQLAPFIASTFPSGKMVLYNSITIGGFKL
                     TTSFDDLRFTNIINDSGSQLGSTVLEYTFGGSYPMGIALGTLAILDSQSSRISKAELY
                     PVLELSFPIINTRLTQFSALLLASGYLPVYPSVKFEEFFDMNSTYFFPNHQLGVGLTV
                     NHKPFNGQIMASLAKGKNHLLLFNDIAEAHDHIDHNGFIDVYASGTWKSKNLTANAIL
                     NVPFTKEFALATVKGETQDADFSQIGLTYTADAFHLSLGASRVGFLRTLKTAFEGGSL
                     KPLFYGKWASSFLEASYTYKIFTFTTRLNVPIILNNQKPSVDISVKVRFDGVF"
     gene            2231..>2507
                     /locus_tag="PKN79_03585"
     CDS             2231..>2507
                     /locus_tag="PKN79_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ94877.1"
                     /translation="MPIARLNPLVAQRIAAGEVIERPSSVIRELIDNSLDAGSTAITV
                     SVTEGGIEEMRVLDDGSGIAKDDLPLLGESHATSKIRDLDDLYHITSM"
BASE COUNT          610 a          701 c          593 g          603 t
ORIGIN      
        1 agagatggaa gcttttttga tgaactgatg gcgttgcgca tggttgatat tcaagcattg
       61 gccatcatca aagtaaagcc atatgtgatt tcacttggag tcagtttggc atacgatgaa
      121 aacactcatc tgacatcgac tatcgacgct gtggtgagga tcgtcctcct ctaaccggag
      181 cgtttaggag agactatgaa acgactagag acctttatcc tcgtaatctt actcaccctc
      241 attgcgccgt taagcatctt tggtcaagag tacgtgagcg tcatcgaagg agatcttgcc
      301 tccttgcgcc tctatgagcc caacggtaaa cccatctcaa tcgaaacaat cgatatcaac
      361 gatcttcagc ttggaacgat cctcgtcagc ggcgacgagc cgatcatctt ggaaaccagc
      421 cgaggagata tcaggctttt ccctgactcc attctgattc tggatgaggt cagtgcgaca
      481 agcactaccc ttttgttgat cgatggatcg gtaacggcga aaacgaccca gggtaccttg
      541 tcaatcatca ccccggcgac cttgtatgaa cttgaccgcg ccggtgaagt gtttgtcact
      601 tcgacccgga atgaggaacg agtccaatcg tttgacgtcg cagtacgcgc cacgaacttg
      661 atcacccata agacaacgac gatcgaagcg ctccaggagc ttgaactctc caatcccaaa
      721 ttggcgaaac gaaccctcgc tccggccgaa gccgccgcag ggcgaattgt cagaccggcc
      781 cggattccgg cacaacccgg tgccatccaa tggactgtgg gattggaatt cgtcgaagaa
      841 gctgaagaaa tcaaagagct tgtccgctct cttcctgcaa aaccggttgt gttgtccccc
      901 agtgtcaaag caatgccggt cgtcaaggaa gctgtcgcca aaccccggac aacccttcgg
      961 gacagtgcgc ctgctcccat ccagtccaaa ggaacctatg gcattgaagc cggatacacc
     1021 ctcaccttcc cgataaaaag cgcttcctat tggccgggcc accgccttga agtgaagccg
     1081 ttcatctcct acaactccct tgcccttcgg ctgaatgtac atattgaaac ggattccttc
     1141 accgcattcg atacgaactt cctgaacctt gatccatccg ctcttgggat tgtttccttt
     1201 gtcttcggca tcgtcgatca tgtgaaagtc ggttattcga caagcccctt ctacctgctg
     1261 attgaagagg gcgggtatcc gggaacgcag ctcgccccct ttatcgcttc caccttcccc
     1321 tcgggaaaga tggtgttgta taactcgatc acgatcggcg gcttcaaact tactaccagc
     1381 tttgacgatt taagatttac gaacatcatc aatgacagcg gaagtcaact cggttcgacc
     1441 gtgttggagt acacctttgg cggatcgtat cccatgggca tcgcattggg aaccttggcg
     1501 atcttggatt cacaaagctc ccggatctcc aaagccgagc tctatccggt gcttgagctc
     1561 tccttcccga tcatcaatac tcgcctgaca cagttttccg ctctcctctt ggcaagcggc
     1621 tatctgccgg tttacccaag tgtaaagttc gaagagttct ttgacatgaa ctccacctat
     1681 ttcttcccga accaccagct tggtgtcgga ctgactgtaa accataaacc gttcaacggt
     1741 cagatcatgg cttctttagc gaaaggaaaa aatcatttgc tgctctttaa cgacattgcc
     1801 gaagcccacg atcacatcga tcataatgga tttatcgatg tatatgcttc aggaacctgg
     1861 aagagcaaaa acctcaccgc caatgcgatt ctcaatgttc cgttcaccaa ggagttcgct
     1921 ctcgctacgg tgaaaggtga aactcaggac gccgacttca gtcaaatcgg cctcacctat
     1981 accgcggatg cgtttcatct cagcttggga gcgagtcggg tcggattcct gcgtacgctg
     2041 aaaacagcct tcgaaggcgg ctcgctcaaa ccactcttct acggcaagtg ggcctcctcg
     2101 tttcttgaag cttcctacac ctataaaatc ttcaccttta ccacgcgctt gaacgtgccg
     2161 attatactga acaaccaaaa accttctgtt gatatcagtg tgaaggtacg attcgatggt
     2221 gtcttctagg atgcccattg cccgcctgaa tccgctggtg gcgcaacgca tcgccgccgg
     2281 cgaggtcatt gaacgtccat catcggtcat ccgggagttg atcgacaaca gtctagatgc
     2341 agggtccacc gcgatcaccg tctcggtgac cgagggggga atcgaagaga tgcgagtcct
     2401 cgatgatggg agcggaatcg ccaaggacga tctgcccttg ctcggcgaaa gccatgcaac
     2461 gagcaaaatc agagatttgg atgacctgta tcacatcacc agcatgg
//
LOCUS       DAOPUZ010000170         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_44039_length_2496_cov_3.786153, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000170 DAOPUZ010000000
VERSION     DAOPUZ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44039_length_2496_cov_3.786153"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKN79_03590"
     CDS             <1..380
                     /locus_tag="PKN79_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94878.1"
                     /translation="NTYLKESMAHEIDSLERMLQQIRDTTLEPGQDRMFTIVPMELTI
                     IVLAAKGDPLSTWTRKVNVAALMHANKKHSWRALSIGYDRKRNLVFADDCPIRREDFT
                     ATDWKFVINAANRLRDKASIERR"
     gene            377..1153
                     /locus_tag="PKN79_03595"
     CDS             377..1153
                     /locus_tag="PKN79_03595"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNZ94879.1"
                     /translation="MKTHLEVALQAARRAGSYALGAERNGAISVRMKEANDYVTDVDI
                     QIEQLIKDTIRLSFPHDGIFGEEGGESGNLQSNRWVIDPIDGTTNFFRGMPNYTISIA
                     WEEKPYNPLVGVVFNPRHNELFWAAKGRGAFLNGQRITVSSVQELHRAIVVLVPPHRR
                     RDLADSYFETMNTIFKATSDIRSFGSCALELSYIASGRVDGYYERALGYYDMAAGMII
                     LQEAGGIVTPAFEDAPFSDHSCDILASNGHLHRALSSLIA"
     gene            1185..1826
                     /locus_tag="PKN79_03600"
     CDS             1185..1826
                     /locus_tag="PKN79_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNZ94880.1"
                     /translation="MKFKLAIFDFDGTIMDTSPGIFSTANATIESLGLEPEKDPSVLA
                     RFVGPPIRESFVAVYNLDESLLDEAVTRYRAIYDRTGRFGAELYEGIDRMLAALKATG
                     YRLAVATLKSEPVVKQMTDHFGLTPYFDSIRGSDPIHKRSKAAVIHQVLEDLQIKAEE
                     AVLIGDTPHDEEGAGEAGSAFIAVDWGFGFPKGYRPSSTMVAESPLDLLTFLL"
     gene            1871..2266
                     /locus_tag="PKN79_03605"
     CDS             1871..2266
                     /locus_tag="PKN79_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNZ94881.1"
                     /translation="MSLQVTKSEVATPNAPKAIGPYSQAVSAGPFVFTSGVLPVDPKT
                     GKLVEGGIVEHTKQVFENLKAILKEAGTSLSNAVKTTVFIASMKDFAAMNEVYASYLG
                     DGVKPSRSTVEIPNLALGALVEIEMIVLQ"
     gene            2276..>2496
                     /locus_tag="PKN79_03610"
     CDS             2276..>2496
                     /locus_tag="PKN79_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNZ94882.1"
                     /translation="MNRTPLFEHYGEHAKLVDFGGWEMPLHFEGGILAEHEAVRKAAG
                     LFDVSHMGECVVTGEEAGAYLDWLLTNRV"
BASE COUNT          595 a          668 c          714 g          519 t
ORIGIN      
        1 tcaataccta cctcaaagaa agcatggctc atgagatcga ttcactggag cgaatgctcc
       61 aacagattcg ggatacgact ctcgaaccgg ggcaggatcg gatgttcacg atcgtcccca
      121 tggagctgac cattattgtt ttggcggcga aaggggatcc gctcagcaca tggacgcgaa
      181 aagtaaacgt tgccgccctg atgcacgcca acaaaaaaca ctcctggcgc gccctttcca
      241 tcggatacga ccgaaagaga aatttggtgt ttgccgatga ttgcccgatc aggcgggaag
      301 attttacagc aacggattgg aagtttgtaa tcaatgcggc gaatcggctg cgggataaag
      361 cgagtataga aagaagatga agacccacct tgaagttgcc cttcaggcag ctcgcagagc
      421 cggctcctac gccttgggag cggagcggaa cggagcgatc tccgttcgga tgaaagaggc
      481 gaacgattac gtcaccgatg tcgatatcca gatcgaacag ctgatcaaag acacgatccg
      541 gctttccttt cctcatgatg gaatattcgg cgaagaaggc ggtgaaagcg gaaaccttca
      601 atccaaccgc tgggtgatcg atccgatcga cggaacaacg aatttctttc gggggatgcc
      661 caactacacg atctcaatcg catgggagga gaaaccatac aacccgttgg tcggggtcgt
      721 cttcaacccc cgtcacaatg aattgttctg ggcggcaaaa ggccggggag cgtttctcaa
      781 cggacaacga atcaccgtct catccgttca ggagctgcat cgggcaatcg tggtgctggt
      841 tccgcctcac cgcaggcgcg acctcgccga ttcctacttt gaaacgatga acacgatctt
      901 caaagccacc agcgatatcc gctccttcgg atcctgcgcc ttggagcttt cctacatcgc
      961 aagcggacgg gtcgacggat actatgaacg ggcgctgggc tactacgata tggcagcggg
     1021 gatgatcatt cttcaggaag cggggggaat cgtcaccccg gcgtttgaag atgctccttt
     1081 cagcgaccac tcttgtgaca tcctcgcttc aaacgggcac cttcatcgag ctttgagctc
     1141 cttgatcgct tgatgaaacc gtttctttcc cgtagggtct tggcatgaag ttcaagctgg
     1201 ccatctttga tttcgacggg acgatcatgg atacctcccc gggtatcttc tcaaccgcga
     1261 atgcgacgat tgaaagcctc gggttggagc cggagaagga tccctcggtc ctcgcccggt
     1321 tcgtcggccc gccgatccgc gaatccttcg tcgcggtgta caacctcgat gaatccctcc
     1381 ttgatgaggc ggttacgcga tatcgggcga tctacgaccg gacgggacgt tttggagccg
     1441 agctctatga agggatcgac cggatgctcg ccgcgctgaa ggctaccggc taccgcctcg
     1501 cggtcgcgac cctgaagtcg gaaccggtgg tgaagcagat gaccgaccac ttcggtctca
     1561 ccccctattt cgattcgatc cgcggcagcg atccgatcca taaaaggagc aaagcagcgg
     1621 tcatccatca ggtcttggag gatttgcaaa taaaagcgga agaagcggtc ctcatcggag
     1681 acaccccaca cgatgaggag ggagccgggg aagcgggcag cgccttcatc gccgtcgatt
     1741 ggggattcgg ctttcccaaa ggatatcgac catcatcgac catggtagct gaaagcccgc
     1801 ttgatttatt aacgttcctc ttgtaagagg ctttgggcct cgatatggta gaggttaagg
     1861 agattatgta atgtcgttac aagtaacaaa gagtgaagtt gccaccccga acgccccgaa
     1921 agcgatcgga ccgtacagtc aggcggtcag tgccggaccg ttcgtattca ccagcggagt
     1981 gctgccggtt gacccgaaaa ccggcaagct cgtggagggc ggaatcgtcg aacacaccaa
     2041 acaggtgttc gagaacctca aagcgatcct aaaggaggcg ggtacgagtc tctccaatgc
     2101 ggtaaagacc acggtcttca tcgccagcat gaaagatttc gcagcgatga atgaagtcta
     2161 cgcctcctat ctgggagacg gggtgaagcc gagccgatca acggtggaaa ttccaaacct
     2221 cgccctcggt gcattggtcg aaatcgagat gatcgtgctc caataggagg gaaagatgaa
     2281 ccggactccg ctttttgagc actatggcga acatgccaaa cttgttgatt tcggcgggtg
     2341 ggagatgcct ctccattttg aaggggggat tctcgccgag catgaagcgg tgcgaaaagc
     2401 cgccggcctg tttgacgttt cccacatggg cgaatgcgtc gtcaccggcg aggaagccgg
     2461 tgcgtacctg gattggctgc tgaccaatcg ggtcag
//
LOCUS       DAOPUZ010000171         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_44041_length_2496_cov_3.657927, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000171 DAOPUZ010000000
VERSION     DAOPUZ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44041_length_2496_cov_3.657927"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PKN79_03615"
     CDS             complement(<1..505)
                     /locus_tag="PKN79_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNZ94883.1"
                     /translation="MKYLGAVDQGTTSTRFIIFDQGGAIVASHQLEHTQIFPQPGWVE
                     HDPMEIWANTQKCITEALKKARLRGRDLSGIGITNQRETIVAWEKESGRVYHNAIVWQ
                     DLRTSDHIEALKDSPNGPVIEQKSGLVLSPYFGGSKIAWLLEHVEGLRSDAESGKAVF
                     GTIDSWLA"
     gene            complement(502..1389)
                     /gene="nfo"
                     /locus_tag="PKN79_03620"
     CDS             complement(502..1389)
                     /gene="nfo"
                     /locus_tag="PKN79_03620"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNZ94884.1"
                     /translation="MYYIGAHTSIAGGLENALLEAQRLNATALGMFTKNQRRWAAKPI
                     EAEEADRFTEVLQETGFSSDRILIHGSYLINLGNPDREKQRRSIRAMADELGRAERLG
                     LKLVNTHPGSTLGLVSKEEGMKIVARGIDEALDESSEAILVIENTAGSGSNLGSTIEE
                     LAGILEATRNHARLGFCLDTCHAHVAGYDLSTVGGFENLIGRFDRLIGLEFLRGLHLN
                     DALNESGSKRDRHASLGEGTIGWPTFEHIVRDPRFRGIPLVLETPDPSIWGDEIAHLR
                     ALTFKNRVCEVYWPKRRNG"
     gene            1443..1937
                     /locus_tag="PKN79_03625"
     CDS             1443..1937
                     /locus_tag="PKN79_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ94885.1"
                     /translation="MGFVFQQMNVLANLNMIDNVVLPALHAKKSRKRELYRRARALMD
                     DFHIGDLAERRVNEVSGGQLQRACICRSLMMEPEIIFADEPTGALNQAAADVIEAFLQ
                     INRKGTSIMMVTHDSRNAAMCDRILYMLDGEIKGELRLGKYVHDEAKEREKRTARWLE
                     EMGW"
     gene            2034..>2496
                     /locus_tag="PKN79_03630"
     CDS             2034..>2496
                     /locus_tag="PKN79_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNZ94886.1"
                     /translation="MAERQLAAGDELYGFILEEITPLAEYQGTGYLFRHIETKMEVYQ
                     VVNEDPECFFGYLFRTLPCNDTGVAHIIEHCLLAGSERYPVHDPFMTLLKGSANTFMN
                     ALTYPDRTVYPAASPLKKDFDNLFAVYTDAVFAPLLREETFWREGVRLVCNE"
BASE COUNT          504 a          740 c          708 g          544 t
ORIGIN      
        1 aggcaagcca ggaatcgatc gttccaaaga ccgctttgcc cgattcggcg tccgaccgca
       61 acccttcgac atgctccaac agccaggcga tcttcgaccc gccgaagtag gggctgagga
      121 ccagtccgct tttttgttcg atgaccgggc cgttcgggct gtccttcaac gcctcgatat
      181 ggtcgctggt ccgaaggtcc tgccagacga ttgcattatg gtagacccgt ccgctctcct
      241 tttcccacgc cacgatcgtc tcgcgctggt tggtaattcc gattccggaa agatccctgc
      301 ccctcaggcg ggcttttttc agggcttcgg tgatgcactt ctgcgtattg gcccagattt
      361 ccatcggatc atgctcgacc caccccggct ggggaaaaat ctgcgtgtgc tcaagctggt
      421 gggaagcgac gatcgcaccc ccctgatcga agatgatgaa tcgggtactg gtcgtccctt
      481 ggtctactgc acctaaatac ttcatccgtt cctcctcttg ggccagtata cttcgcacac
      541 ccggtttttg aaagtcaaag cgcgcagatg ggcgatctca tcgccccaga tcgagggatc
      601 gggtgtttca agaaccagcg ggattcctct gaagcgggga tcccggacaa tatgctcgaa
      661 cgtcggccat ccgatcgtcc cttcgcccag gctggcatga cggtcgcgtt tgctgccgct
      721 ttcgtttaag gcatcgttga ggtgaagacc tcgcaaaaat tcgaggccga tcaaccgatc
      781 aaagcggcca atcaggttct cgaaccctcc gaccgtcgaa agatcgtagc cggctacgtg
      841 ggcgtggcag gtatccaggc agaaccccag gcgggcatgg ttcctcgtcg cttccaaaat
      901 tccggcaagc tcctcgattg tcgagccgag gtttgaaccg ctgccggcgg tgttctcaat
      961 gacgaggatc gcctccgagc tctcatcgag cgcctcatcg attccccgag cgacgatctt
     1021 catcccctcc tccttggaga cgaggcccag cgtggagccg gggtgggtgt tcaccaactt
     1081 cagacccagc cgctcggcgc gcccaagttc atcggccatc gctcgaatcg aacgtcgttg
     1141 tttctcccga tcggggttgc cgagattgat caggtagctt ccgtggatca gaatccggtc
     1201 ggaactgaag ccggtttcct gcaacacctc cgtaaatcga tccgcctctt ccgcttcgat
     1261 cggtttggcc gcccatctgc gctgattctt cgtaaacatc cccaaggcgg ttgcgttcaa
     1321 gcgctgcgcc tccaagaggg cgttttccag tccaccggcg atcgaggtat gggctccgat
     1381 atagtacatg gttccacggt agggctcagc ccacctcttg tcaatcgatt tgtaccggta
     1441 ggatgggctt cgtgtttcag cagatgaacg tactggcgaa tttgaacatg atcgacaacg
     1501 tcgtgctgcc ggcgctccat gcgaagaaga gccggaagcg ggaactctac cggcgcgccc
     1561 gggccctgat ggatgatttc cacatcgggg atcttgccga gcggagggtc aacgaggtgt
     1621 ccggcggaca gctgcagcgg gcgtgcatct gccgcagtct gatgatggag cccgagatca
     1681 tcttcgcgga cgaacccacc ggagcgctga atcaggcggc cgccgacgtg atcgaggcgt
     1741 ttttacagat caaccggaaa ggaacctcaa tcatgatggt cacccacgac agcaggaacg
     1801 ccgcgatgtg tgatcggatc ctctacatgc tggacggcga gatcaaaggt gaactgaggc
     1861 ttggaaaata tgtccacgat gaagcgaagg aacgtgagaa aagaaccgcc cggtggcttg
     1921 aggagatggg gtggtaacct gcccggcaaa tgtgatgatg ctccgatcgg gtgtttccgg
     1981 cgctcttgtc aattgatttg aggcgtgtta gtgtcctgta acggaggtca tccatggctg
     2041 aacgacagct cgccgcaggc gacgagcttt acggattcat tttggaagag atcaccccct
     2101 tggcggaata tcagggaacc gggtacctct ttcgacatat tgaaacaaag atggaagtct
     2161 accaagtggt caacgaagac cccgaatgct tcttcggcta tctttttcga acccttccct
     2221 gcaatgatac cggcgtagcc cacatcatcg agcactgcct cctggcggga tcggagcgct
     2281 atccggtgca cgatcccttc atgacgctgc tcaaaggcag cgccaatacc ttcatgaacg
     2341 ccctcaccta tcccgaccgg accgtgtacc cggccgccag tcccctgaag aaggacttcg
     2401 acaatctttt tgccgtctac accgatgcgg tcttcgctcc cctgctccgg gaggagacgt
     2461 tctggcggga gggggtgcgc ctcgtctgca acgagg
//
LOCUS       DAOPUZ010000172         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_44152_length_2492_cov_3.076734, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000172 DAOPUZ010000000
VERSION     DAOPUZ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44152_length_2492_cov_3.076734"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKN79_03635"
     CDS             <1..181
                     /locus_tag="PKN79_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNZ94887.1"
                     /translation="TEKKVLELLVKNSDMIADELADAIGVSERTIQRAYASLQKNGYL
                     ERIGSKKSGKWIVLK"
     gene            complement(437..1237)
                     /locus_tag="PKN79_03640"
     CDS             complement(437..1237)
                     /locus_tag="PKN79_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ94888.1"
                     /translation="MRIIQTAVGPKRVPLTGYLQDMTMDGGIRNIRPTIIVCPGGGYT
                     FRSERERDPVALHFLAMSYNVFILDYSVGEDAGDLNPLLELSDALMRIREHAVRWMCD
                     PTRIAVIGFSAGAHLAASIAVLHDHKRVTDVQKVVDANNRPDAVILAYPVITGKEYAH
                     EGSFDRLSRGNAEIRNLLSLENQVTETATPAFIWHTVTDQGVLVENSLLLAQAYRKAK
                     VPFELHLFEEGDHGLSMCTEEVGTPHAGVRPWVELATNWLNRRFMHNL"
     gene            1300..2223
                     /locus_tag="PKN79_03645"
     CDS             1300..2223
                     /locus_tag="PKN79_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNZ94889.1"
                     /translation="MKQKVILDVDTGLDDAAALFLAAGAEELEIVAVIATAGNVGLDK
                     TVENTLNVIETTGLSCPVFAGSAKPLIRQPVEAGDFHGESGLDGPVFGKRVIQELQEE
                     DGVSAMIRLVKRYPGEVTIISVGPLTDLAHALRREPALPSLVKEIIVMSGSFSGGNVT
                     KDAEFNAYADPEAAAIVFSSGAPMVMLTLDITRTVTLSRERLDSLRTIEGRSAEVFSQ
                     CMDTYMANYEAKKFGWPQMHDPLCVAYAIDRSRFTAEYQRVIVDIDPASPSYGMTSRE
                     AIDGEGGVVIPTDVDRPWFWEMVERSLRNLP"
     gene            complement(2220..>2492)
                     /locus_tag="PKN79_03650"
     CDS             complement(2220..>2492)
                     /locus_tag="PKN79_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ94890.1"
                     /translation="PYYEFLRDNHRLEEWLRSQGYLAIANMMTGARSLGIQSCAIEGF
                     DEEGVLRVLGIDPEEWLVSLLCTFGHAAEEERPKIRVPLDELVTCY"
BASE COUNT          582 a          689 c          681 g          540 t
ORIGIN      
        1 aacagaaaag aaggttctgg agcttttagt taaaaattcc gatatgatag ctgatgaact
       61 tgcagatgca attggagtct cagaaagaac tatacaacgg gcatatgctt ctttgcaaaa
      121 aaatggatat ttagaaagaa tcggttctaa aaaaagcgga aaatggattg tcttaaaata
      181 ggttcacatt gcaggtatgt aacccgacac cctccatgtc gggttacatg tcaggttaaa
      241 aatcaacggt gaaataatgt gatgaataca ttattagagc acccgtagcg tatgaatgaa
      301 ctatttgctc ttgaacttct caagcacctt gcttcagttt taaaccctcc atatcaagtt
      361 gtttttccaa ctctgatatg atgtttcccc aaagacatct cacaagcatg gacgagccga
      421 atccgggctc aaggggttat agattgtgca tgaagcggcg attcagccaa ttggtcgcaa
      481 gctcgaccca cggtctgact ccggcatggg gggtgcccac ctcttcggtg cacatggaaa
      541 gcccatgatc cccctcttcg aagaggtgca gctcgaaggg aaccttggcc tttcggtagg
      601 cttgggcaag aagcaggctg ttctccacca agaccccctg atcggttacg gtatgccaga
      661 taaaggcggg agtcgcggtc tctgtcacct gattctccag acttagaagg tttcgaatct
      721 cggcgtttcc ccggctcaac cggtcgaagc ttccctcatg ggcgtattcc ttgccggtga
      781 tcaccggata cgcaaggatg accgcatccg ggcggttgtt ggcgtccacg accttctgca
      841 catcggttac ccgcttgtga tcatgaagga cggcgatcga ggcggccagg tgcgcgccgg
      901 cggagaaccc gatcaccgcg attcgggtgg ggtcgcacat ccacctgacg gcgtgctcac
      961 ggattcgcat cagcgcatcg ctgagctcca ggagggggtt aaggtctccg gcatcctctc
     1021 caaccgagta atcgaggatg aagacgttgt agctcatcgc taggaaatga agcgccaccg
     1081 gatcccgttc ccgttcactg cggaacgtgt atccgccccc ggggcagacg atgatcgtgg
     1141 ggcgaatgtt ccggattcct ccgtccatcg tcatatcctg cagatagccg gtcaacggga
     1201 cccgcttcgg tcccacggcg gtttgaatga ttctcatgcg cccatgatac cacaaaagat
     1261 tcattgcgtt gagcgaaaag agttgatagg atgtgatcca tgaaacagaa agtgatcttg
     1321 gatgtcgata cgggattgga tgatgcggcg gcgctgtttt tggccgccgg tgcagaagag
     1381 cttgagatcg tcgcggtcat cgccaccgcc ggcaatgtcg gtcttgacaa gaccgttgaa
     1441 aacaccctca acgtgatcga gaccaccggc ctttcgtgcc cggtcttcgc cggatccgcc
     1501 aagccgctga tccgccagcc ggtcgaagcg ggcgacttcc acggcgagtc ggggctggac
     1561 ggaccggtct tcggaaaacg ggtcattcaa gagcttcaag aagaggacgg ggtctccgcc
     1621 atgatccgcc tcgtcaaacg atatcccggg gaggtcacga tcatcagcgt cggcccgctg
     1681 accgatcttg cccacgcctt acgaagagaa cccgcccttc cctcgctggt gaaggagatc
     1741 atcgtcatga gtgggtcgtt cagcggcgga aacgtcacca aggacgccga attcaacgcc
     1801 tatgccgacc cggaagcagc cgctatcgtc ttttccagcg gagcgccgat ggtcatgctt
     1861 actctggata tcacccgaac cgtgacgctc tcaagagaac gactggattc tctgaggacg
     1921 atcgagggac ggtcggcgga ggtcttcagc cagtgcatgg acacctacat ggcaaactac
     1981 gaagccaaga agttcggctg gccgcagatg catgatccgc tctgcgtggc gtatgccatc
     2041 gaccgaagca ggttcactgc cgagtatcaa cgggtcatcg tcgatatcga tcccgcctcc
     2101 ccctcctacg gcatgacgag cagggaagcg atcgacggcg aaggcggagt agtcattcct
     2161 accgatgtcg accgcccctg gttctgggag atggtggaac gttccttaag aaaccttcct
     2221 taataacagg taaccaattc atccaagggc actcgaatct tcggtcgctc ctcttcggca
     2281 gcatggccga aggtgcaaag aagagaaacg agccactcct cggggtcgat tcccaagacc
     2341 ctgagcaccc cctcctcgtc aaacccctca atcgcgcagc tttggattcc gagtgatcgg
     2401 gctccggtca tcatgttggc gatcgcaaga tacccctggc ttcggagcca ctcttccagc
     2461 cgatgattgt ctcttaaaaa ctcatagtag gg
//
LOCUS       DAOPUZ010000173         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_44186_length_2491_cov_2.929803, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000173 DAOPUZ010000000
VERSION     DAOPUZ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44186_length_2491_cov_2.929803"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..362
                     /locus_tag="PKN79_03655"
     CDS             18..362
                     /locus_tag="PKN79_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ94891.1"
                     /translation="MSVPEGASGTVLAEREQRARSVADRARSGADFTALAKEFSAFAG
                     SLGVEEATLATAWVLANEAITAPIIGPTNEDHLRLSLKALDFEITKEIATRLDEIAPP
                     PPPAHDRSEVDR"
     gene            399..1814
                     /locus_tag="PKN79_03660"
     CDS             399..1814
                     /locus_tag="PKN79_03660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNZ94892.1"
                     /translation="MDDITSLARERFGITAVRPFQYLVIKRILEEDKKENPHPGMIVT
                     LPTGSGKSLCFMLPALAVSGLTIIVYPLLALMSDQVRRFEEAKIPVLCLKGGQSKKER
                     MEILSDLTNVRALVTNAETLAHPRVLSALLHIPISMIVIDEAHTIERWGSSFRPALKT
                     LSSLIAHLRPKQTLCFTATADNAVIALLNTLFFTGKPAHLVRGSSDRENIAYYSYPTL
                     SKSRAITTLLHNPSTRPAIVFAPTRALTERLARYCRISNPTLDARHYHAGLPKDARHM
                     IESWYFHHPDGVLFATNAYGMGMDKKDIRTVIHHSPPSDALAFLQESGRAGRDGLPAR
                     SIVLINEADLSSTLFPIFNQHKQCYRSLLLSLIDEELEHCFGCGVCLNHRILLREGER
                     PILRTIFFNPLKYTPYTLALVLQKKDARVLYSGSLSTWRIREIEEAVRTLVQEGRILQ
                     PPFLKGKMILLPQIDKSRRDR"
     gene            1843..>2491
                     /locus_tag="PKN79_03665"
     CDS             1843..>2491
                     /locus_tag="PKN79_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNZ94893.1"
                     /translation="MATVREVQNGALHRRYPEFYGNVYDEAEHDRRYQTLIETHAKLF
                     SEENPILFSTAGRSELGGNHTDHNKGRVIAATINLDTIAAVTKRNDSIVTIISEGYPP
                     VTVDLNDLVVKKEEYNTTESLVRGIAFAFKERNLAIGGFCANTSSKVLKGSGLSSSAA
                     IEVLCGTIFNHLYNDDVLKPVDLAQIGQFAENTYFGKPSGLMDQLACAYGGIIGID"
BASE COUNT          540 a          757 c          670 g          524 t
ORIGIN      
        1 acctgggcca catcctgatt tcggtgcctg aaggcgccag tggcaccgtg ctggcagagc
       61 gcgagcagcg cgcccgcagc gtggccgatc gcgcacgcag cggcgccgat ttcaccgccc
      121 tggccaagga gttttccgca ttcgccggat ccctgggcgt cgaggaggcg acgcttgcca
      181 ccgcatgggt tcttgccaac gaggcgatta ccgccccgat catcggaccg acgaacgagg
      241 atcacttgag gctaagcctc aaagcgctcg actttgagat cacaaaagaa atcgcaacga
      301 gactggatga gatcgctccc ccgccgcccc cggcgcatga ccgcagcgag gtcgacagat
      361 agctgatgcg aaccggcaac tgattgcgaa tacggggtat ggatgacatc acctcccttg
      421 cccgtgagcg cttcggcatc actgcggtgc ggcccttcca atatctggtg attaaacgaa
      481 ttcttgaaga ggacaagaag gagaaccccc accccgggat gatcgtgacg ctgcccaccg
      541 gaagcggaaa gagcctctgc ttcatgcttc ccgcattggc ggtctcgggt cttaccatca
      601 ttgtctatcc tcttttggcc ttgatgagtg atcaggtgcg aaggttcgag gaggcgaaga
      661 tccccgtcct ctgcctcaaa ggagggcagt cgaagaagga acgaatggag attctttccg
      721 atcttacgaa cgtccgcgcc cttgtcacca acgctgaaac attggcccat cctcgtgtgc
      781 tatccgccct gctgcacatc cccatctcga tgatcgtcat cgatgaggcg catacgatcg
      841 agcgctgggg ttcctcgttt cggccggcac tgaagacgct tagctccctt atcgcccatc
      901 ttcggccgaa gcagaccctg tgcttcaccg ccaccgccga caatgcggtc atcgccttat
      961 tgaacaccct ttttttcacc ggaaagccgg cccacctcgt tcgaggttca agcgaccggg
     1021 aaaacatcgc ctactattcc tatccaaccc tttcaaagtc ccgcgccatc actaccctcc
     1081 tgcacaatcc ctcgacccgg ccggcgattg tctttgcccc gacccgtgct ttgacggagc
     1141 ggctcgcccg gtattgccgg atttccaatc cgaccctcga tgcccgccat taccatgccg
     1201 gccttccaaa ggatgcccgg cacatgattg aatcatggta tttccaccat cccgacgggg
     1261 tgctcttcgc cacgaacgcc tacgggatgg ggatggacaa aaaggatatc cggacggtaa
     1321 tccatcattc tcccccctcc gacgcccttg cgtttctgca ggaaagcgga cgggcggggc
     1381 gggatggcct gccggctcga tcgatcgttc tgatcaatga agccgacctc tcttcaaccc
     1441 tctttccgat cttcaaccaa cataaacaat gctaccgctc gctcctgctt tcgctgatcg
     1501 atgaagagct cgagcactgc ttcggctgtg gagtctgctt gaatcaccgg attcttctca
     1561 gggaggggga acggccgatt ctgcgcacca ttttcttcaa tccgctgaag tacaccccct
     1621 atacgcttgc cttggttctg caaaaaaagg atgctcgtgt tctctattcc ggctcgcttt
     1681 ccacctggcg gatccgggag atcgaggaag cggtcaggac ccttgtccaa gaaggaagga
     1741 ttcttcaacc acccttcctg aaagggaaaa tgattctcct gcctcagatt gacaagagca
     1801 ggagggaccg atagagtttt ttacagacga gggaggatcg gcatggcgac ggtacgtgaa
     1861 gtccaaaacg gtgcattgca ccggcggtat ccggaattct atgggaatgt gtatgacgaa
     1921 gcggagcacg accggcggta tcagaccctg atcgaaaccc atgccaagct gttttccgaa
     1981 gagaatccga tcctcttctc caccgccggc cgcagcgagc tcggcggaaa ccataccgac
     2041 cacaacaagg ggcgggtgat cgccgccacg atcaatctcg atacgatcgc cgccgttacg
     2101 aaacgaaatg attcgattgt taccatcatc agcgaagggt atcccccggt aacggttgat
     2161 ctgaatgatc tggtcgtaaa gaaagaggag tacaacacca ccgagagttt ggttcggggt
     2221 attgcgtttg cgttcaagga gcgcaacttg gcgatcggcg gcttttgcgc caacacctcg
     2281 agcaaggtgc tgaaaggttc cggcctctcc tcctcggcgg cgatcgaggt gctgtgcgga
     2341 acgattttca accatctata caacgatgat gtgttgaaac cggtcgactt ggcgcagatc
     2401 gggcaattcg ccgagaacac ctacttcggc aagccttccg gactgatgga ccagcttgcc
     2461 tgcgcctatg gaggaatcat cggcatcgac t
//
LOCUS       DAOPUZ010000174         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_44557_length_2476_cov_4.124742, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000174 DAOPUZ010000000
VERSION     DAOPUZ010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44557_length_2476_cov_4.124742"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKN79_03670"
     CDS             <1..303
                     /locus_tag="PKN79_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNZ94894.1"
                     /translation="YDQYPPKVAAWARLAGNVLIIVTFAVLVIPSVNYAIFVSFQKTA
                     ALRINYFWIFIPFTYFLLSIIGYTIEPVIEDWKIITGKLDDSAERKMDQLMKEVKA"
     gene            300..1589
                     /locus_tag="PKN79_03675"
     CDS             300..1589
                     /locus_tag="PKN79_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNZ94895.1"
                     /translation="MSFSLLMTLIVFVLIFFLRMPIAIGMISSAVVYLLVKGADLSMV
                     VNQVMNTFFTNYVIIAVPLFIFTANVMNTGKVTDMIYNFATSVMGRVRGALGHVNILG
                     SLLFSGMTGSAIADAAGLGKIEIEAMRDAGYEVEFACAMTAASATIGPTFPPSIPLVI
                     YAMLSSTSVGALFLSGVVPAVLLTIFLMIYVAIVSHIRNYPRGEKIVWKVFLKNTLSA
                     IPALLTPVILLIGIYTGVMTPTEAGAIAGLYALLISFFAYRAMGIKDLLEVLKTTIKD
                     IGSTSIMIGAATVISYIVAREQLAQNIGNWILSLTTSKYMFLFIVNVIILILGMFMDT
                     STIQLVFVPIMIPVARALGIDMIHFGVIVTYNMMVGLSTPPFGMLLFITSGITATPLK
                     NIVREIWWPLVVMLILLFIITYLPELSLFLPRAAGLL"
     gene            1600..>2476
                     /locus_tag="PKN79_03680"
     CDS             1600..>2476
                     /locus_tag="PKN79_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNZ94896.1"
                     /translation="MKDTVYKGCWPTMITPFTSDNKIDYPAVEALTRWYIDRGADGIF
                     AVCQSSEMFFLSEEEKTDLAKAVVEAADGAIKVIASGHTADEHAKQIDQLGKMGETGV
                     DAVVLVSNRLAKEHEGEDLFRKNIEDIFAQLPGVTFGMYECPYPYLRLLDDEFLRWAA
                     TEDKLVFLKDVSCSLEIQRRRVELVKGTNLALFNANTATVLDSWLMGYHGYNGVMANF
                     HIDLYAWLFEHYKENPVLAREVMDHLTVWAVAEARSYPVNAKYHRNLTGVPMTLATRT
                     KNPSLLNENGRHEIAS"
BASE COUNT          533 a          730 c          662 g          551 t
ORIGIN      
        1 tatgaccaat atccgccgaa agtcgccgca tgggcgcgtc tggcgggtaa cgtgctgatc
       61 atcgttacct tcgccgtgct cgtcatcccc tcggtgaact acgcgatctt tgtaagcttc
      121 cagaaaaccg cagcccttcg gatcaactat ttttggattt tcattccatt cacatacttc
      181 cttctttcca tcatcggata cacgatcgaa ccggtcatcg aggattggaa gatcatcacc
      241 ggaaagctgg atgacagcgc cgagcgcaaa atggatcaat tgatgaagga ggtgaaggca
      301 tgagtttttc cctgttgatg accctgatcg tctttgtcct gatcttcttc cttcggatgc
      361 cgatcgccat cgggatgatc agcagcgccg tggtctatct gcttgtaaaa ggtgccgatc
      421 tctccatggt ggtaaaccag gtgatgaaca cctttttcac caactatgtc atcatcgccg
      481 ttcccctgtt catcttcacc gcgaacgtga tgaacacggg aaaggtcacc gatatgatct
      541 acaacttcgc caccagcgtg atggggcggg tccgcggagc cctcgggcat gtgaacatcc
      601 tcggctccct cctcttcagc gggatgaccg gctcggcgat cgccgatgcg gcggggctgg
      661 gaaagatcga aatcgaggcg atgagggacg ccggctatga ggtcgagttc gcctgcgcga
      721 tgaccgccgc ttcggcgacg atcggaccaa ccttcccgcc ttcgattccg ctggtcatct
      781 acgcgatgct ttcatcgacc agcgtcggag cgctctttct aagcggagtg gttcccgccg
      841 ttctgttgac gatcttcctg atgatctacg tcgcgatcgt ctcccacatc cgcaactatc
      901 cccgcggcga gaagatcgtc tggaaggtgt ttttgaagaa caccttgtcg gcgattcccg
      961 ccctcctcac cccggtgatt ctcctcatcg gaatctacac gggagtgatg acccccaccg
     1021 aagcgggtgc gatcgccggc ctgtatgcgc tgctcatctc cttcttcgct tatcgggcaa
     1081 tgggaatcaa ggacctcttg gaggtgttga aaacgacgat caaggatatc ggatcgacca
     1141 gcatcatgat cggagcggcc acggtgatca gctacatcgt cgcccgcgag caacttgccc
     1201 agaacatcgg caactggatt ctcagcctta caaccagcaa gtacatgttc ctcttcatcg
     1261 tgaacgtcat catcctgatt cttggaatgt tcatggatac ctccacgatc cagctggtgt
     1321 tcgtccccat catgattccg gttgcccggg ccctcggaat cgacatgatc cacttcggag
     1381 tgatcgtaac gtacaacatg atggtcggtc tttccactcc gcccttcgga atgctgctct
     1441 tcattaccag cggaatcacc gctaccccgc tcaagaatat cgtacgcgag atctggtggc
     1501 cgctcgtggt catgctgatc cttctgttca tcatcaccta cctccccgag ctcagcctct
     1561 ttttgccccg agccgcggga ttgttataag gatgctgcaa tgaaagatac ggtatataag
     1621 ggctgttggc ccacgatgat caccccgttc acctccgaca acaagatcga ctatccggcg
     1681 gttgaagccc tgaccagatg gtatatcgac cggggggcgg acggaatctt cgccgtatgc
     1741 caatccagcg agatgttctt cctctccgaa gaggagaaga cggatcttgc caaggcggtg
     1801 gtcgaggcgg ccgacggggc gatcaaagtg atcgccagcg gacacaccgc cgacgagcac
     1861 gccaagcaga ttgaccagct gggaaagatg ggcgaaacgg gagtcgatgc ggtggtgctg
     1921 gtctccaacc gcctcgccaa agagcatgag ggagaggatc tcttccgaaa aaacatcgaa
     1981 gacatctttg cccaattgcc gggcgtaaca ttcggcatgt acgagtgccc ctacccctat
     2041 ctgcgcctcc ttgatgacga gttcttaaga tgggcggcga cagaggacaa gctggtcttc
     2101 ctcaaggatg tctcctgttc gctggagatc cagaggcggc gggtcgaact cgtcaaaggc
     2161 acgaatctgg ccctcttcaa cgccaacaca gcgacggtcc tggactcgtg gctgatgggg
     2221 tatcacggat acaacggagt gatggcgaac ttccacatcg acctctacgc gtggctcttt
     2281 gagcactaca aagagaatcc ggtactcgct cgtgaggtga tggaccacct caccgtgtgg
     2341 gcggtcgccg aggcgcgctc gtatccggtg aacgccaagt accaccgaaa cctcaccggg
     2401 gttccgatga ccctcgccac ccggaccaag aatccctcgc tgctgaatga gaacggaaga
     2461 catgaaatcg ccagcc
//
LOCUS       DAOPUZ010000175         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_44815_length_2466_cov_3.985068, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000175 DAOPUZ010000000
VERSION     DAOPUZ010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44815_length_2466_cov_3.985068"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1498
                     /locus_tag="PKN79_03685"
     CDS             86..1498
                     /locus_tag="PKN79_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94897.1"
                     /translation="MKKLLVLVLLVAFSTPLFSFDFFATLDDEEGQVGTEPAKPKELT
                     SLFVEGFGTYYPLDEDEPFDYGISLGVTFEENRERIRTEVDFSFSPILRDPLSVRTLA
                     VTFFFGPTNLKVGMFTHPWGTSTTLHAVDVLNSQDLTHGILDNPEAMKKSEFMIVYNA
                     YFKRSSLDLIIKPFYRPIDMEASDRYAPMDTDNLMGTSFSYGGMEIPIDSDNIRVGIY
                     EHPKFWKALGGGGRYRFGIGDLDLGFMYFSSFYPQMGFDIGAKLKMIANPYSDDPPLI
                     PDKVEVTSIDFDFTRYHFAGTEGSLLVGPVTIAWEGGLFLSEDVKGEKPEKYNTKAMA
                     VVELSYTEPKTSFFASAAYQGQYLFGDVTPTVNQTLDVIIPVDYIASFLSDKPYSSSA
                     AVAFEMPFMRERLKVRMAATYGIENQSLVALGSLSYEINDSMSVYARGAYYNGFGDKK
                     SLFKNWENNSSVTIGMNAWF"
     gene            1536..>2466
                     /locus_tag="PKN79_03690"
     CDS             1536..>2466
                     /locus_tag="PKN79_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ94898.1"
                     /translation="MLILSDILLFLTAALVFSVTCLVILLRVRVADALTTDFLRVLVP
                     LSLQMLLSLLATYLERSLSPRQLASFSYTMYALGATIISILLTTAMLFAFTRFFIGRL
                     PASEKQKRLGNIILRLLIILFFLISIWAILGKSRGDWIKALDETMKYHFFSASTFLVI
                     HAVVALFFIKQASTWEEEWLLKGVAMTFLPLIVLFPLDILFFLHLSFKVVYLSFAILS
                     VHLYFFISRRYFLTYEQPALPKVNLTERFGITEREQEILRLLADGLSNQEIAKQLFIS
                     VNTVKTHIKNIYAKLEVNNRLQLFNLMKDRDSNA"
BASE COUNT          508 a          740 c          626 g          592 t
ORIGIN      
        1 gagatggttc aggtccgcta tgatgtcccc attccggccg cgtatttcac agttcgtttc
       61 ttagaaacag ggaggctgta atccgatgaa gaaactgctt gtactggtat tgcttgtcgc
      121 cttttcaacc ccgctcttct ccttcgactt ctttgcgact ctggatgatg aagagggcca
      181 agtcggcacc gagccggcga agccaaaaga gctcacgtcc ctctttgtcg aggggttcgg
      241 cacctactac cccctggatg aggatgagcc gttcgactac ggcatctcgc tgggagtgac
      301 ctttgaagag aaccgggaga gaatccggac cgaggtcgac ttctccttca gtccgattct
      361 tcgcgacccc ctctccgtcc gcaccttggc ggtcaccttc ttcttcggcc cgacaaacct
      421 caaggtgggc atgttcaccc acccgtgggg gacgagcacg accctccacg ccgtggatgt
      481 ccttaactcg caagacctca cccatggcat tctggacaat cccgaggcga tgaagaaaag
      541 cgagttcatg atcgtctaca acgcctactt caagcgctca tccctcgacc tcatcatcaa
      601 gcccttctac cggccgatcg acatggaagc atccgaccgt tatgcaccga tggataccga
      661 caatctcatg ggtacctcct tcagctatgg aggaatggag attccaatcg actcggacaa
      721 catccgggtc ggaatctacg aacatccgaa gttctggaaa gcccttggag gcggaggccg
      781 ctatcgcttc ggcatcggcg atctggatct cggcttcatg tacttctcca gcttctaccc
      841 gcagatgggc ttcgatatcg gtgcgaagct gaagatgata gccaatcctt attccgatga
      901 tcctccgtta attcccgata aagtggaagt cacatccatc gacttcgact tcacccgcta
      961 ccacttcgcc ggcacggagg gttcgctcct cgtcggtccg gtcacgatcg cctgggaagg
     1021 cggcctgttc ctcagcgagg atgtcaaagg agagaagcct gaaaagtaca ataccaaggc
     1081 gatggcggtt gtggagctct cttatacgga gccaaagacc tccttcttcg cttcggcggc
     1141 gtatcagggt cagtatctct tcggtgatgt aacaccgacg gttaatcaga cacttgatgt
     1201 catcatcccc gtcgattaca tcgcctcctt cctgagcgac aagccctact ccagtagcgc
     1261 ggcggtcgcc ttcgagatgc cgttcatgcg cgaacggctc aaggttcgga tggcggcgac
     1321 gtacggcatc gagaaccaga gcctcgtcgc gctgggatcg ctcagctatg agatcaatga
     1381 ttccatgagc gtgtatgccc gaggcgctta ctacaatggc tttggtgaca agaagagcct
     1441 cttcaagaac tgggagaaca acagctcggt gacgatcgga atgaacgctt ggttctaacg
     1501 gttttttcat ggtaggatga ccgaacggag ggcatatgct cattctttcg gacatcctgc
     1561 tctttttgac cgcggctttg gtcttttccg tcacctgcct cgtcatcctt ctgcgagtcc
     1621 gcgtcgccga tgcgctgacc acggactttt tacgggttct cgtccccctg agcctccaga
     1681 tgctgttaag cctcctcgct acctaccttg agcgatccct ttctcctcgc cagctggcga
     1741 gcttttccta tacgatgtac gccctcgggg cgacgatcat ctcgattctc ctcaccacgg
     1801 cgatgctctt tgcgttcacc cgcttcttca tcggccggct gccggcgagt gagaagcaga
     1861 aacggttggg gaacatcatc ctgcgcctgc tcatcatcct cttctttctg atctccatct
     1921 gggcgattct cgggaagagc aggggcgatt ggatcaaggc gctggatgaa acgatgaagt
     1981 atcatttttt cagcgcctcc acctttctgg tcatccatgc ggtcgtggcg ctcttcttca
     2041 taaagcaggc ttcgacctgg gaggaggagt ggctgctgaa aggggtcgcg atgaccttcc
     2101 tgccgctcat cgtcctcttt cccctggata tcctcttctt cctccacctc agcttcaagg
     2161 tggtctacct aagcttcgcg atcctctcgg tccatctcta cttcttcatc agcagaaggt
     2221 acttcctcac ctacgagcaa ccggctcttc ccaaagtgaa cctgaccgaa cggtttggca
     2281 tcaccgagcg ggagcaggag atcctccgcc tcctggcgga tggcttatcc aatcaggaga
     2341 tcgccaagca gctcttcatc tcggtcaata cggtgaagac ccatatcaaa aacatctacg
     2401 cgaaactgga ggtgaacaac cgcctgcagc tgttcaatct gatgaaggac cgcgactcga
     2461 acgctt
//
LOCUS       DAOPUZ010000176         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_44924_length_2462_cov_3.404653, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000176 DAOPUZ010000000
VERSION     DAOPUZ010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44924_length_2462_cov_3.404653"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PKN79_03695"
     CDS             <1..558
                     /locus_tag="PKN79_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94899.1"
                     /translation="VPVSSPSGTNAGHATTPIIPGGAMSGGDPGQGGGNGGIVYGDEP
                     ISDPVVHTLFSLESEDVPIILSQFTGTQVKQINTATLEVVVENGTGGEKISVDVLFTD
                     DSPVGWRGFHLLHETPGPTIPYNLYWGDTTTRIMKDSPIQWNNLVAGEHTKTIYMGRI
                     DETFVNQAAEGRYKSTITVTLTNSN"
     gene            complement(765..1247)
                     /locus_tag="PKN79_03700"
     CDS             complement(765..1247)
                     /locus_tag="PKN79_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNZ94900.1"
                     /translation="MTSIHCLITIALAVLPISELRGAIPYAYFNGAPLFFAALIGALA
                     NLLVAPIVYLFLGSLHRVFYRIWGWYRSFFDRFVERARKKVEHHFSKWGYIGLALFVG
                     IPLPVTGAWTAALGAWVLGLKKKETILAISLGVLISATIVTLIIAFGADVDSIFIKRI
                     "
     gene            complement(1244..2362)
                     /locus_tag="PKN79_03705"
     CDS             complement(1244..2362)
                     /locus_tag="PKN79_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNZ94901.1"
                     /translation="MSCVSIVRCESYEPALVDKAVREACASAGMPDVSGKTVLLKPNI
                     LSDSKEELGITTHSQVVRSVIRLLKEQGAKRILVGDSPGLHTPNFRPRASGIWKVIRE
                     EGVEWADFTDRPTTYPIPYARGKKLPLPSLLEEVDLLFSLPKMKTHQLMYATGGIKNL
                     FGLVPNLHKSPCHLSFPTREQFASLMVGIATVAKASFALMDGIIAMEGPGPANGSSLP
                     MGMILASTDLVALDYAQAEIMGYNPTDVPIVAEGLKRGLGKAPSEYPNLQAHDLIKTD
                     YKRIEVQKKTRFFHALILPFFAGPFVRWRVKRQRRAPFFLKEECILCKKCIQICPVDA
                     LRIEEKHIVIDEKRCIRCYCCHEVCPASAILVDEEIPS"
BASE COUNT          611 a          645 c          692 g          514 t
ORIGIN      
        1 gttcctgtct caagcccctc gggaaccaat gccggacatg ccacgacccc gatcatcccc
       61 ggcggagcga tgagcggggg agaccccggc caaggaggcg gcaacggggg aatcgtctac
      121 ggggatgaac cgatatcaga tccggtcgtc catacgctct tttcgctgga gagtgaggat
      181 gttcccatca tattgagcca atttaccgga actcaggtaa aacagatcaa tacggcaaca
      241 ttggaagtgg tggttgagaa cggaacgggt ggtgagaaga tcagcgtgga tgtactgttc
      301 acggacgact cgcctgtcgg ctggaggggg tttcatcttc ttcacgagac acccggtccc
      361 acgattccct acaacctcta ctggggggat accacaaccc ggatcatgaa ggactctccg
      421 attcaatgga ataatctggt agcaggagag cataccaaaa ccatttacat gggccgaata
      481 gatgaaacct tcgtcaatca ggccgctgaa ggcagataca aaagcaccat taccgttacc
      541 ctaaccaaca gcaactagcc aatcttcata ttttccaaga gtttagctct caagcgacta
      601 ccggaaaccg gtaattcatt tttgaaatgc ttggttcagt gtttttattt caccatgaag
      661 cagaatgata ctatcggaag taaaatagta ataatatcgg aagtgaaata gtaatattat
      721 cggaagtaga gtcttcttct tggaagattg ttgatgagag cacgtcagat ccgtttgatg
      781 aagatcgaat cgacgtccgc tccgaaggcg atgatcagcg tgacgatcgt ggcggagatg
      841 aggactccca aagagattgc caagatcgtc tccttcttct tcaggcccaa cacccaggcg
      901 ccaagggcgg cggtccatgc ccccgtgacg ggtaagggaa tgccgacgaa cagggcaaga
      961 ccgatgtatc cccatttgga gaagtgatgc tcgacctttt ttcgtgcccg ctcgacgaag
     1021 cggtcgaaga aggagcgata ccacccccaa atccggtaga acaccctgtg cagcgatccc
     1081 aaaaagagat agacgatcgg agcgaccaga agatttgcaa gagctccgat cagggcggca
     1141 aagaagaggg gagcgccgtt gaagtaggcg tagggaatcg ctcctctgag ttcgctgatc
     1201 gggaggacgg ctaaagcgat cgttatcaga cagtggatgg acgtcacgaa ggaatctcct
     1261 catcgaccag aatagcggag gcgggacaca cctcgtgaca acaataacac cggatgcacc
     1321 gcttttcgtc aatcactata tgcttctctt caatcctaag agcgtcaacg ggacagattt
     1381 ggatgcattt tttacagagg atgcactctt ccttcaggaa gaagggggct ctgcgctggc
     1441 gtttgacccg ccagcggacg aaaggtccgg caaagaaggg gagaatcagg gcgtggaaga
     1501 aacgggtctt cttctgtact tcaatccgct tatagtcggt cttgatcagg tcatgcgctt
     1561 ggaggttcgg atattcgcta ggcgcttttc ccaacccccg cttcaatccc tcggcgacga
     1621 tcgggacatc ggtcggattg tagcccatga tctccgcctg ggcatagtca agagccacca
     1681 gatccgtcga ggcgagaatc atgcccattg gcaggcttga gccgttggcc ggaccgggac
     1741 cttccatcgc gatgattcca tccatcaggg caaaagaagc cttggcgacc gtggcgatgc
     1801 cgaccatcag ggaggcgaac tgctcccggg tcggaaagct taagtggcac gggctcttgt
     1861 ggaggttcgg aaccagaccg aagaggttct tgatcccacc ggtggcgtac atcagttggt
     1921 gggtcttcat cttcggcagg gagaaaagga ggtcgacctc ttccaacagg gagggaaggg
     1981 gaagcttctt gccgcgggcg tagggaatcg gataggtggt cggacgatcg gtgaaatccg
     2041 cccattcgac cccttcttcc cggatcacct tccagatccc gctggctcgg ggtcgaaagt
     2101 tcggggtatg gagccccggc gaatctccga caagaatccg tttggctccc tgctccttca
     2161 gcaagcggat gacacttcga acgacctggc tgtgggtggt gatgccgagc tcctctttgg
     2221 aatcggagag gatgttaggt ttcagcagaa ccgtcttgcc gctgacatcc ggcatccccg
     2281 cagaggcgca cgcctccctg accgccttgt ccaccaaggc aggttcatac gattcacacc
     2341 gaacaatgga aacacaactc atgtgagcct ccgaatcaag cacatataca acggtccctt
     2401 tccatccgag acatcgggaa ggatgatcga accgaaggat cgtttctctc cccggtcgag
     2461 gt
//
LOCUS       DAOPUZ010000177         2460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_44990_length_2460_cov_3.046154, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000177 DAOPUZ010000000
VERSION     DAOPUZ010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44990_length_2460_cov_3.046154"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2248)
                     /locus_tag="PKN79_03710"
     CDS             complement(<1..2248)
                     /locus_tag="PKN79_03710"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNZ94902.1"
                     /translation="MNQAYDDQYTVHQQFDEEGMTIRELLSIFRKRFFWFLLGLVLVS
                     GAAVGYLFWATPLYESQVSVLVNPIQNSSSIDSLLSVTSSGAKIATEVELITSRSNIN
                     EALARLDLTRYENDEGESYAMNIAFPNSVKEKISVTTVKDTNIVRISVTDANRFFARD
                     FANVLAESYDSMLTSIARNSKTAQRTFIESQIPINDQALREANDRLGDFKERSEIIQL
                     SDKSSLLVNQISYYDLRLEPLKLQLEEAYIGFRSHEPFIAASGTTSDQLLTMIRSDEE
                     ISKKLSELESWLLELAMYESLPTNLGQGALSEGTLARIFTINNAIDTTKKSLLNRVRK
                     LIECCYIDSLAQNIAQILTTGVSIDILQGRAAVFEEELSRLPLLERTLSELLRDVQIS
                     EAIGLKLREMLEEIKLLEASVSGNVKVIDTANLPLKPVSPNSLLILAVALLLGSAIGL
                     LLALLVESLDTSIQNEEQVQRIVGRNAPMLGWVPIMKTYQKDVYPMFMVRTHPNSFEA
                     ERLKLIANMIYSMDGKKVFSITSSSMAEGKSMIVANISLALAQLGNKVLVVDGDLRLP
                     SMERFFRLQHQSVGLVDAVTSGVSLEDCIVQPLEDVSTLHLLPAGSSPLVPAAIFSNP
                     RYISWTKRLSNIYDYIIIDAPPLDAASELLAIAKHVDGLIITVRAGVTSRIALRDLLE
                     SLINANVQLAGFVFNGVMPSKHSRYGTYGYSVSYKMGKVERKFGRKYRYFPRRRTASW
                     YRKRYKQDV"
     gene            complement(2232..>2460)
                     /locus_tag="PKN79_03715"
     CDS             complement(2232..>2460)
                     /locus_tag="PKN79_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94903.1"
                     /translation="SVKVQGEDGRKIAKTEEEVPPSSTIIVKKNTFTKDIAPTVAIVG
                     LVAAILGIVSTTLSIIKDVLTIIGVANESGI"
BASE COUNT          542 a          640 c          685 g          593 t
ORIGIN      
        1 tgacgtcctg tttgtaccgt ttgcgatacc acgaagcggt tcgccttctg gggaaatagc
       61 gatatttccg cccgaacttt cgttcgacct ttcccatttt ataagaaacc gagtagccat
      121 atgttccata gcggctgtgc tttgaaggca tcactccgtt gaagacgaag ccggcgagct
      181 gaacattcgc gttgatcagg ctttccagaa ggtcgcgcag agcgatacgg ctggtcactc
      241 ccgcccggac ggtgatgatc aagccgtcaa cgtgtttggc gattgcaaga agctcgctcg
      301 ccgcatcgag cggaggagcg tcgatgatga tgtaatcgta gatattggaa agccttttcg
      361 tccaggatat atatcttggg ttggagaaaa tcgccgccgg caccaagggt gacgatccgg
      421 cgggaagcag gtggagggtg cttacgtctt caagcggttg gacgatgcaa tcctccaagg
      481 aaactccgct ggtaaccgcg tcgaccaacc cgacactctg gtgttgaagg cggaagaagc
      541 gttccatgct cggcaggcgc aagtctccgt cgacgaccaa taccttgttg cccagttggg
      601 ccaaagcgag ggagatgttc gccacgatca tgctctttcc ctcagccatc gaactgctgg
      661 tgatcgagaa gacttttttt ccgtccatcg aatagatcat gtttgcgatg agcttgagcc
      721 gttccgcttc aaacgagttc ggatgggtac ggaccatgaa catcggatac acatcctttt
      781 gatatgtttt catgatcgga acccacccca gcatcggggc gtttcgaccg acgatccgtt
      841 gaacctgttc ctcattctgg atcgatgtat ccaaggactc caccaacagg gcgagcagca
      901 gtccgatcgc cgaacccaaa agcagggcca ccgccaggat caggagggag ttgggactga
      961 ccggcttgag cggcaagttc gccgtgtcga tcactttcac gtttccgctg actgaagctt
     1021 ccagaagctt gatctcctca agcatctccc gaagcttcaa tccgatcgct tcgctgatct
     1081 gaacatcccg aagcagttcg gaaagggtgc gctccagaag gggcaagcgg gaaagctctt
     1141 cttcaaacac cgcagcccgg ccctgaagaa tatcgatcga caccccggtc gtgagaatct
     1201 gggcgatgtt ctgggccagc gaatcaatgt agcagcattc gataagcttt cgaacccgat
     1261 tcagaaggct cttcttcgtc gtatcgatcg cgttgttgat ggtgaaaatc cgggcaagcg
     1321 ttccttcgga aagggctcct tgaccgaggt ttgtcggtag cgactcgtac atcgccagct
     1381 ccagcaacca cgattcaagc tcactgagct tctttgaaat ctcctcgtcg cttctgatca
     1441 ttgtcagcag ttgatcggag gtggtgccgc tggcggcgat gaaaggttcg tgggagcgga
     1501 agccgatata cgcttcttca agctgaagct taagaggctc gagtcgaagg tcgtagtagg
     1561 atatctggtt taccagcagt gagctcttgt cggaaagctg gatgatctcg cttcgctcct
     1621 tgaaatctcc aagtcggtcg ttcgcctctc tgagggcctg atcgttgatc gggatctggc
     1681 tctcgatgaa ggtccgctga gcggtctttg agttccgggc aatggaggtg agcatactgt
     1741 catagctctc agccagaaca ttggcaaaat cccgggcaaa gaagcggttt gcgtcggtga
     1801 cgctgatgcg gacgatattg gtgtccttca ccgtggtgac ggagatcttc tccttcaccg
     1861 agttgggaaa cgcgatgttc atcgcatagg attcaccttc gtcgttttca taccgggtga
     1921 gatcaagccg cgccagagcc tcgttgatgt tgctgcggga ggtgatgagc tcgacttcgg
     1981 tcgcgatctt cgcccccgag gaggtgacgc tgagcagcga atcgatcgat gaggagtttt
     2041 gaatcggatt gacgaggacg gaaacctgag actcgtagag cggagtcgcc cagaagagat
     2101 acccgaccgc agcgccgctt accagcacga gaccgagaag aaaccagaag aaccgcttgc
     2161 ggaagatcga gagaagctct ctgatcgtca ttccttcttc gtcgaattgc tggtgaactg
     2221 tatattggtc atcatatgcc tgattcattc gctactccta ttatagtgag cacatctttg
     2281 atgatgctca acgttgtgga cacgattccc aatattgcgg caaccagtcc gacaatggct
     2341 acagtcggtg cgatatcttt cgtaaaggtg ttcttcttga cgatgatcgt cgagctcggg
     2401 ggaacttcct cttcggtttt ggcgattttc ctgccgtcct caccttggac tttcaccgac
//
LOCUS       DAOPUZ010000178         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_45016_length_2459_cov_3.123128, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000178 DAOPUZ010000000
VERSION     DAOPUZ010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45016_length_2459_cov_3.123128"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="PKN79_03720"
     CDS             complement(<1..1477)
                     /locus_tag="PKN79_03720"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNZ94904.1"
                     /translation="MKQLVLAEKPSVGRELARVLGCTQRSEGFIEGSEYIITWALGHL
                     IELAEPAAYSDSYRRWSLKDLPMLPPELKLEVIDQTKEQFRIIKSLVERADIASLIIA
                     TDAGREGELVARWIIEESGYRGPIKRLWISSQTEEAIQTGFANLKEGSLYDNLYRAAF
                     SRAAADWYVGMNVTRALTCHYDARLSAGRVQTPILALMVDREDEIEAFNGRFYWTLKA
                     RFGPISATLWPTEETSRIETEELANEYRARIVGKTGTVSEVYRDEKTMPSPLAYDLTA
                     LQRDANTLLDFSAKETLDILQGLYERHKVVTYPRTDSRYITSDILPTLNRRIESLTNT
                     VLGPVARQYLTGGYGFDEGRFVQDAKVTDHHAIIPTEIWVNPASLSKKENDLFNLIAL
                     RFLEVLSSSYRYESILTTIESENLVFKSRSTRPLDLGWRRVALHSGLKSAEFVDEEEY
                     HTEEVAAGQNLPVLDAALRRFTTPAPLRYTEATLLSAMENAG"
     gene            1627..2418
                     /locus_tag="PKN79_03725"
     CDS             1627..2418
                     /locus_tag="PKN79_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNZ94905.1"
                     /translation="MEPKHRTTARILDILELVAAHPEGVSLAFISQRLSIPKSSLHPL
                     LLTLTDRHYLYHKAKEERYYLGESLFVVGNKYVNDADILGEIRNIVFDISKELGETLY
                     FGVLSDLDVLYLIKADLYSQFRVVSTPGNKLPAYSTGYGKALLSQFGPEEIRSFYPDG
                     KLKPVTDRTITDTDELNRQLEEVRRCGFSFEKGESTEGIQCVAVRIEHDDEVLAGLSV
                     AVPEFRYTEEREKLFKKALKETKHKIEQVIEQQRNQWIYSGEADR"
BASE COUNT          527 a          689 c          709 g          534 t
ORIGIN      
        1 gtccggcgtt ctccatcgct gaaagcaggg tggcttccgt gtagcggagg ggcgcaggcg
       61 tggtgaaccg cctgagcgcg gcatcaagaa cgggaagatt ctgtccggcc gctacttctt
      121 ccgtatggta ttcctcttca tccacgaact cggcgctctt caggccgctg tgcagggcga
      181 cccttcgcca ccccaagtcg aggggtcggg tcgaccggct tttgaagacg agattctcgc
      241 tttcgatcgt ggtgaggatc gactcgtagc gataggagga ggaaagcacc tccaggaagc
      301 ggagggcgat caggttgaag agatcgttct ctttcttcga cagggaagca ggattcaccc
      361 atatttcggt agggatgatc gcatggtggt cggtgacctt cgcatcctgg acgaagcggc
      421 cttcatcgaa gccgtatccc ccggtgaggt actgccgggc gaccggaccc aaaacggtat
      481 tggtcaacga ttcgatcctc cggttgaggg ttggcagaat gtcggaggtg atgtagcggc
      541 tgtctgttcg gggataggtt accaccttgt gccgctcata gaggccctgg aggatgtcca
      601 gcgtttcctt tgcgctgaaa tccaaaaggg tgttggcgtc ccgctgaagg gcggtcagat
      661 cgtacgccag gggggaaggc atcgtctttt catcccgata tacctcgctc accgttccgg
      721 tcttgccgac aatcctcgcc cggtattcat tggccagctc ctcggtctcg atccggctgg
      781 tctcttcggt gggccacagg gtggcggaaa tcggaccgaa ccgggctttc aaggtccagt
      841 agaagcgacc gttgaaggct tcgatctcat cttcgcggtc gaccatcagc gcgaggatcg
      901 gggtctgcac cctgccggcg gagagccggg cgtcgtagtg gcaggtaaga gcccgggtaa
      961 cgttcatgcc gacgtaccaa tccgccgcgg cccggctgaa cgccgcccgg tagaggttgt
     1021 catacaggct tccctctttg aggttggcga atccggtttg aatcgcctct tccgtctggc
     1081 tgctgatcca caggcgcttg atcgggcccc ggtagccgga ctcttcgatg atccagcggg
     1141 caaccagctc cccttcccgg ccggcatcgg tggcgatgat cagggaagcg atgtccgccc
     1201 gctcgacgag ggacttgatg attcgaaact gctctttcgt ctgatcgatc acttcgagct
     1261 tcagctcggg gggaagcatc ggaagatcct tcagcgacca ccgcctgtag gaatcgctgt
     1321 aggccgccgg ctcggccagt tcgatcagat ggccaagagc ccaggtaatg atgtactcgg
     1381 atccttcaat aaacccttca ctgcgctggg tgcaccccaa cacccgggca agctcccgcc
     1441 cgacactcgg cttttccgcc aacaccaact gtttcaaaga ttcctccacc ttcgtgataa
     1501 taccgtccgg cacgcaggct tgcaaggcgg gacggtgtga taaggggttt acttccgaac
     1561 aatatggtgt atacttatat acggatacag gaacagcgtt cgtatatgcg aacacaagag
     1621 gggtttatgg agccgaaaca ccggacaaca gctcgaattc tcgatatctt ggaacttgtt
     1681 gccgctcacc cggagggagt gtcgcttgcg ttcatctccc agcggctttc cattcccaaa
     1741 tcaagcctgc acccccttct ccttactctt accgatcgcc actatctcta ccacaaggcg
     1801 aaggaggaac ggtattacct tggagagagt ctcttcgtcg tcggaaacaa gtatgtgaac
     1861 gatgcggaca tccttggtga aattcgaaac atcgtctttg acatctccaa ggagctgggt
     1921 gaaaccctct acttcggagt actctccgac ctcgatgtat tgtatctgat caaagccgat
     1981 ctctacagcc agtttcgggt cgtctccact ccgggaaaca agctgcccgc ctactcgacc
     2041 ggctacggca aagcgctttt aagccaattc ggacccgaag agatccgctc cttctacccc
     2101 gacggcaagc tgaagccggt gaccgaccgc accatcaccg atacggatga actgaatcga
     2161 cagctggaag aggttcgacg gtgcgggttc tccttcgaaa agggggaatc aaccgaaggg
     2221 atccagtgtg tcgccgtgcg gattgagcac gatgacgagg ttctggccgg cttatccgtt
     2281 gcggtgcccg agtttcgcta cacggaagag cgggaaaagc tctttaaaaa agcgctcaaa
     2341 gaaacaaaac acaagattga gcaggttatt gaacagcagc gcaatcaatg gatatatagc
     2401 ggagaagcag atcgatgaag cgtgaagtca ttacaatcgg tgaaagcctc gtagcgttt
//
LOCUS       DAOPUZ010000179         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_45590_length_2438_cov_3.201846, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000179 DAOPUZ010000000
VERSION     DAOPUZ010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45590_length_2438_cov_3.201846"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PKN79_03730"
     CDS             <1..708
                     /locus_tag="PKN79_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNZ94906.1"
                     /translation="AFGAALMIIFAGASSLSIQLSAMIFGLLGLVFSYYLSTRFRFGG
                     PLLRLVLAGIAVSAFFSSGLGVIKLVAEPTKDLQDITFWMMGGLWNASWGSIIPTLVV
                     IAIALTILHMNRFTLNLLSINEKTSFSLGLNPKRQRVILLVVATVGTTFTISVTGLIG
                     WVGLITPHIARKTFGSEATHALPASMVMGAFFLLLCDSFGRTILPTEIPIGLLTSLFG
                     ALIFVLILSGKSQEGKA"
     gene            705..1460
                     /locus_tag="PKN79_03735"
     CDS             705..1460
                     /locus_tag="PKN79_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ94907.1"
                     /translation="MTFLSIEKLSYQYPNGINALQSVTLSIDKGSSVAILGSNGAGKS
                     TLLDILLGYKPSTSVSLYGKPLSTYGRKELGRLIALVPQKEQFSFAYTLLDYTLFGRA
                     PYLSPMGIPTSSDNLIAYEALAKVGLGRFENRSITALSGGEQQLLLIARAIAQESEIL
                     LLDEPTSSLDPANRKRILTLLKQLHSEGKTLIFTTHDANFAYELSSDIALLKGGTLLG
                     YGKKEDVVKSELLTTLYDAQLTVTEIDGRTIIY"
     gene            complement(1454..>2438)
                     /locus_tag="PKN79_03740"
     CDS             complement(1454..>2438)
                     /locus_tag="PKN79_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ94908.1"
                     /translation="IGLLGGVDSKNSRRCCFCTDDRQPVSILEEGHINNNLRIAVHEG
                     LDPIEAICMATINSGECYNLTDRGAIAPGRRADFCLADDLVEFKMHQVYVGGVLVAQE
                     GKMLKPSKAKKDQRVASKMNIKDFSVERLDLRLKHNRVRVIDIVPGGVVTEQGEAFVK
                     LEKGLWVHDPNQDVVKLAVIERHKGLGTVGVGLIRGFGLRKGAIASTVGHDSHNLIVA
                     GDNDADMAKAVEHLIETGGGMTVIQNGKALSTLEMPVAGLISYEKGEVVAEKLQHLHD
                     MAIEKLSVKPGIDAFTTLSFMALPVIPTYKLTDMGLFDVGTFSFVDLELDQ"
BASE COUNT          511 a          734 c          600 g          593 t
ORIGIN      
        1 gcgttcggag cggccctgat gatcatcttc gccggcgcat cttcgctctc catccagctt
       61 tcagcgatga tcttcggact cctgggattg gtgttttcct attatctttc cactcgattt
      121 cgttttggag gaccattgtt gcgcctcgtc ttggcgggga tcgccgtctc ggccttcttc
      181 tccagcggcc tcggggtgat caagctcgta gccgaaccga cgaaggatct tcaggacatc
      241 accttttgga tgatgggcgg actctggaac gcttcctggg gttctatcat tccgactttg
      301 gtcgtaatag cgatcgcgtt gacaattctc catatgaatc gtttcaccct caatcttctt
      361 tcaatcaatg aaaaaacttc tttttccctg gggctcaatc caaaacgtca gcgagtcatc
      421 ctgctcgtcg tcgccacggt gggaacaacg ttcacaatca gcgtgaccgg cctcatcgga
      481 tgggtcgggc tgatcacccc tcatatcgcc cgaaagacct tcggcagcga agcgacccac
      541 gcccttcccg cctcgatggt catgggtgcc ttcttcctgc ttttatgtga ttcgttcgga
      601 agaacgattc ttccgacgga aatacccatc gggttattga ccagtctctt cggcgcgttg
      661 atcttcgtcc tcatcctttc cggcaaaagc caggagggca aggcatgacg ttcctgtcaa
      721 tagaaaaact ctcctatcaa taccccaatg gaatcaatgc gcttcaatcc gttacgcttt
      781 caattgacaa aggctcctcc gttgcgatcc tcggatccaa cggggcggga aaatcaacgt
      841 tgctcgatat acttttagga tacaaaccga gcacttcggt ttctctttat ggcaaaccgc
      901 tttcgacgta tggccggaag gagctcggtc ggctcatcgc ccttgtcccc caaaaggagc
      961 agttctcttt tgcctacacc ctcctcgatt acaccctctt cggtcgggct ccataccttt
     1021 ccccgatggg catccccacg agcagcgata acctgatcgc ctatgaagcg ttggcgaaag
     1081 tcggcctcgg ccgctttgaa aaccggagca tcaccgcgct ttccggagga gagcagcagc
     1141 tcctcctcat cgcccgggcg atcgcccagg aatcggagat tctgctactc gacgaaccga
     1201 cctccagcct ggatccggcg aataggaaac ggattctcac cctgctcaaa cagctccact
     1261 ccgaggggaa aacactcatc ttcacgaccc atgacgcaaa ctttgcgtat gagctctcaa
     1321 gcgatatcgc tttgttgaaa ggcggcacgt tgcttggcta cggcaagaaa gaagacgtgg
     1381 tcaaaagcga acttctgacc acgttgtacg atgctcaact aaccgtaacc gagatcgacg
     1441 gtcggaccat catctattga tccagctcca aatcgacgaa gctgaacgtt ccgacgtcaa
     1501 agaggcccat gtcggtcagc ttatacgtcg ggatgaccgg cagcgccatg aagctcagcg
     1561 tggtgaacgc atcgatgccg ggtttcaccg agagcttttc aatcgccatg tcgtggaggt
     1621 gttgcagctt ctctgcgacc acctcgccct tctcatagct gatgaggccg gctaccggca
     1681 tctccagagt ggaaagcgct tttccgtttt gaatgacggt cattccgccg cccgtttcga
     1741 tcaagtgttc gacggctttg gccatatccg catcgttgtc gccggcgacg atgaggttat
     1801 gcgaatcatg tccgaccgtt gaagcaatcg ctcctttgcg cagtccgaat ccgcggatta
     1861 atccgactcc gaccgttccc agtcccttgt gccgctcgat gaccgcaagc ttgaccacat
     1921 cctgattggg atcgtggacc cacaacccct tctccagctt gacgaacgcc tcgccctgtt
     1981 ccgtcaccac cccgccggga acgatgtcga tgacccggac ccggttgtgc ttcaaccgaa
     2041 gatcaagacg ctcaacggaa aaatctttga tgttcatctt cgatgcaacg cgctgatcct
     2101 tcttcgcttt tgatggcttg agcatctttc cttcctgagc gacaagaact ccacccacat
     2161 atacttggtg catcttgaat tcaacaagat catcagcaag gcagaagtcc gcccgtcttc
     2221 cgggggcgat cgccccgcga tcggtcagat tgtagcactc gccggagttg atcgtcgcca
     2281 tgcagatcgc ttcaatcggg tcgagacctt catgaacggc gatgcggaga ttgttgttga
     2341 tatggccttc ttcaaggatg ctgaccggct gtcgatcatc ggtgcagaaa cagcagcggc
     2401 ggctgttctt cgaatcgact cctcccagca gcccgatg
//
LOCUS       DAOPUZ010000180         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_46192_length_2415_cov_2.916102, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000180 DAOPUZ010000000
VERSION     DAOPUZ010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46192_length_2415_cov_2.916102"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /gene="pgi"
                     /locus_tag="PKN79_03745"
     CDS             <1..215
                     /gene="pgi"
                     /locus_tag="PKN79_03745"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014342442.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNZ94909.1"
                     /translation="PSSLLYAKRLTPEVLGTLLAHYENKVMFQGFVWNLNSFDQEGVQ
                     LGKVLATKVLQGAAGDEILQAYRSLF"
     gene            313..2043
                     /locus_tag="PKN79_03750"
     CDS             313..2043
                     /locus_tag="PKN79_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ94910.1"
                     /translation="MIPALRKQAVGNRLPTFLTILFVLLEVAMDVTIPYLMANLLDRG
                     ISGASMSEIIFWGAILLLCSFVALAFGILSGHFAARASTGFARNVRKSLYHKIQEFSF
                     ANLDRLPSSSLVTRLTTDVTNVQMAYQMLTRIAVRSPAMLFFSFFMAYTINASLSLVF
                     LVAIPTLALGLFLLIRAAYPIFTKVFKTYDRLNTVVQENIRSIRVVKSFVREEHEIEK
                     FNGVNTEIYKQFSKAEKIIAFNSPLMQGTMYISILVISYIGAKLITSSSMTTGQLMSF
                     ITYTSQILMSLMFLSMVVVMLTISRAAIQRIEEVLREESDITEKADAVTTVKDGSIIF
                     DDVSFSYTNHADKLNLEGITLDIPSGSTVGILGPTGSGKSSLVQLLARLYDVYSGSVK
                     IGGVDVRDYSIKSLRDSVGVVLQKNTLFSGTVAENLRWGNEDASQEELIRVAKIAQAH
                     PFINELEEKYDSVVEQGGTNFSGGQRQRLAIARALLKRPKILILDDSTSAVDTKTEAS
                     IRAALKNELKETTKIVIAQRISSVIDADQIIMLENGKIHAVGSHETLLGSCARYQEMY
                     ASQMRKEERA"
     gene            2040..>2415
                     /locus_tag="PKN79_03755"
     CDS             2040..>2415
                     /locus_tag="PKN79_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94911.1"
                     /translation="MRGPGMHGGGGGPKGRVPVKLDFGTFRRLLAYIFVPYKVRFVIV
                     VIAILLSSVAGVAGSLFLKVLIDDHITPMVAQGSGASFAPLLRAIGVMASIYLVGIVS
                     TLLYNLLMIEIAQGSLKSIRLKM"
BASE COUNT          512 a          716 c          659 g          528 t
ORIGIN      
        1 gcccctccag cctgctgtat gccaagcgcc tcacccccga ggtgctggga acgctcctgg
       61 cccactacga gaacaaggtg atgttccaag gctttgtctg gaacctcaac tccttcgacc
      121 aggaaggggt tcagctcgga aaggttctcg ccaccaaggt ccttcagggg gccgcggggg
      181 atgagattct ccaagcctat cgaagtctct tctgatccga agccccttcg ggggcttttt
      241 tcaggagatt cgatcccttt tcctcctctt tcggttgtct acgataccca agttcggtac
      301 tatcggaagc acatgatacc ggcattgaga aaacaagcgg tgggaaaccg ccttccgacc
      361 tttctgacca tcctcttcgt ccttctcgag gtcgccatgg acgtcacgat cccctatctg
      421 atggcgaatc tgcttgatcg gggaatcagc ggcgcatcga tgagcgagat catcttctgg
      481 ggggcgatcc tgcttctttg cagctttgtc gcccttgcct tcggaattct cagcgggcac
      541 ttcgccgccc gggcttcgac ggggttcgcc cgcaacgtac ggaaatccct ctaccataag
      601 attcaggagt tctccttcgc aaacctggac cgcctgccct cctccagcct ggtcacccgc
      661 ctcaccaccg atgtcaccaa tgtccagatg gcctaccaga tgcttacccg gatcgccgtc
      721 cgcagtccgg cgatgctctt cttcagcttc ttcatggcgt acacgatcaa cgcctcgctg
      781 agcctggtct tcctggttgc gatcccgacc ctcgccctcg gcctctttct gctgatccgc
      841 gccgcctacc cgatcttcac gaaggtgttc aagacctatg accgcctgaa tacggtggtt
      901 caggagaaca tccgctccat ccgggtcgtg aagtccttcg ttcgggaaga gcatgagatt
      961 gaaaaattca acggggtgaa cactgaaatc tacaagcagt tctccaaagc ggaaaagatc
     1021 atcgccttca acagcccgct gatgcaggga acgatgtaca tctcgattct cgtgatcagc
     1081 tatatcggag cgaaactcat cacctcatcg tcgatgacca ccggccagct gatgagcttc
     1141 atcacctaca cctcacagat tctgatgagt ctgatgttcc tcagcatggt ggtggtgatg
     1201 ctcacgatca gccgtgcggc gatccagcgg atcgaggagg tcctcaggga agagagcgac
     1261 atcactgaaa aagccgatgc cgtcacgact gtcaaagacg gttcgatcat cttcgacgat
     1321 gtctcgttca gctatacgaa ccatgctgac aagctgaacc tcgaagggat cactctggac
     1381 attccctccg gttccacggt gggcattctg gggccgacgg gaagcggaaa gagctcgctc
     1441 gtgcagctgc ttgcccgcct gtatgacgta tactcgggaa gcgtgaagat cgggggggtc
     1501 gatgtccgcg attattcgat caagagcctt cgcgacagtg tcggcgtggt tctgcagaag
     1561 aacaccctct tcagcggaac ggtcgccgag aacctccggt ggggaaacga ggacgcctcg
     1621 caggaggagt tgatccgggt ggcgaaaatc gcccaggccc atccgttcat caatgagttg
     1681 gaggaaaaat acgacagcgt cgtcgaacag gggggaacga acttctccgg agggcagcgc
     1741 caacggcttg cgatcgcccg ggcgttgttg aaacgtccca agattctgat tcttgacgac
     1801 tcgaccagcg cggtcgatac gaagaccgaa gcttcaatcc gcgccgcgtt gaagaatgaa
     1861 ttgaaggaaa cgacgaagat cgtcatcgcc cagcggatca gttcggtcat cgacgccgat
     1921 caaatcatca tgctggagaa tggaaagatt catgcagtgg ggagccatga gacgctcctc
     1981 gggtcgtgcg cccgctatca ggagatgtac gccagccaga tgcggaagga ggaacgagca
     2041 tgagaggtcc cggaatgcat ggcggcggtg gcggtcccaa aggccgagtc cccgtaaaac
     2101 tggatttcgg aacgttcagg cgcctccttg cctacatctt tgttccgtac aaggtccgtt
     2161 ttgtcattgt tgtgatcgcg atcctcctca gctccgtcgc cggagtagcc ggctcgctct
     2221 ttctgaaagt cctcatcgat gaccatatca ctccgatggt cgcccaaggc agcggcgcct
     2281 ccttcgcccc gctcctaagg gcgatcgggg taatggcctc catctacctc gtcggaatcg
     2341 tctcaacgct gctctacaac ctgttgatga ttgaaatcgc tcagggctcg ctcaagtcga
     2401 tccgcttgaa gatgt
//
LOCUS       DAOPUZ010000181         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_46359_length_2410_cov_3.024628, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000181 DAOPUZ010000000
VERSION     DAOPUZ010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46359_length_2410_cov_3.024628"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PKN79_03760"
     CDS             <1..781
                     /locus_tag="PKN79_03760"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNZ94912.1"
                     /translation="KIVFSALPAEIAREAEPQLRDNGFYVFSNASSMRYDPNVPILIP
                     EANLDQLDLIKKQGYPEKGFVVTNANCSTTGLAMALAPLMKYGIKSVTMHSYQSVSGA
                     GYPGLSSFDITDNCIPYIGGEEEKVEREIKKILAFDPEVFCYTVRVPVMFGHLEAVWI
                     DTEKDLSIEEVKEAWSAFKLEAEYPSSAEHPVQYGEANTFPQPKYAFWGKPSGMVVYT
                     GRLKKKNGKIGFILLVNNVVKGAAGGSIQNAEAFVSKYGLV"
     gene            781..1290
                     /locus_tag="PKN79_03765"
     CDS             781..1290
                     /locus_tag="PKN79_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNZ94913.1"
                     /translation="MDEYFDLVDRERRSLNRIHRRGTPLEAGTYHNVVSVWTMNRDGR
                     VLLTLRSANRPLMPNLWENTSGWVLTGESSVEAALRELEEETGIEATPEQTTLLGTLL
                     KIQSFVDVYLVRVDIDPKSIRLQKEEAISYRWEDEAGLQVLHEEQLLAFPIAFEFEPF
                     RTALRQAAL"
     gene            complement(1259..1993)
                     /locus_tag="PKN79_03770"
     CDS             complement(1259..1993)
                     /locus_tag="PKN79_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94914.1"
                     /translation="MPLSTLKKIRRTFYRSLDQRFKRYVDQPLDLGDVEKKAWEQLPK
                     RSLLVDGDRLPYLDPTHLRKQVGKGKPLEELLFTDGSFLYFPLAPITFLEERFFEDLT
                     WLIDRASDDGCLERIRFGLNNIAHIRFFEKRRLPVFCDIYLYLANSQSARLIGETRLN
                     LIGGYHWLERLEGDTALWPFEPTFVDPAFQAPLFISRSCFRYDSLGLGCEGCPRSGSW
                     YVTQPDKKAHVIVRECITTLLGEERS"
     gene            2134..>2410
                     /locus_tag="PKN79_03775"
     CDS             2134..>2410
                     /locus_tag="PKN79_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94915.1"
                     /translation="MGLGGARLALNGELRIFQNDGEVDRFLIGGNLFGNERDVQIVAA
                     DQMEFRADRRPFRIVRERNPNPRSRLGKADTFDGKANRLKQSETVFRK"
BASE COUNT          571 a          625 c          699 g          515 t
ORIGIN      
        1 gaagattgtc ttcagcgcgc taccggctga aatagcccgc gaagcggagc cgcaattgcg
       61 ggacaacggc ttttatgtgt tctccaacgc gtcttcgatg cgctacgatc ccaacgttcc
      121 gattctcatt cccgaggcga accttgatca gctggatctg atcaaaaagc agggatatcc
      181 cgaaaaagga ttcgtggtga ccaacgccaa ctgttcgacg accggattgg caatggctct
      241 cgccccgttg atgaagtacg gaatcaagtc ggtcacgatg cactcctacc agagtgtcag
      301 cggagccggg tatcccggtc tttcctcctt tgacatcacc gacaactgta taccgtatat
      361 cggaggcgaa gaggagaagg ttgagaggga gatcaagaag attcttgcct tcgatcccga
      421 agtgttctgc tacaccgttc gagtcccggt gatgttcgga caccttgaag cggtgtggat
      481 cgataccgag aaggatctct caattgaaga ggtcaaggaa gcgtggagcg ccttcaagct
      541 tgaagccgaa tacccctcca gtgccgagca ccccgtgcag tacggtgaag cgaacacctt
      601 cccccagccg aagtatgcgt tctggggaaa gcccagcgga atggtcgtgt acaccggccg
      661 gctgaagaag aagaacggaa aaatcggatt cattcttctg gtaaacaacg tcgtgaaagg
      721 agccgcaggc ggttccattc aaaacgcgga ggcgttcgtc agcaagtacg gcctggtgtg
      781 atggatgagt atttcgacct tgtcgacagg gaacgacgat cattgaatcg aatccacagg
      841 cgtggtacgc ccttggaagc agggacgtac cacaatgtgg tttccgtgtg gacgatgaac
      901 agggacggaa gggtattgtt gactcttcgc tcggcgaaca gacctctgat gccgaacttg
      961 tgggaaaaca cctccggttg ggtacttacc ggtgaaagca gtgtcgaggc cgctctccgc
     1021 gaactggagg aagaaaccgg gattgaagcg acaccggagc agacgactct cttgggaacg
     1081 ctcttaaaaa tacaatcctt tgttgatgtc tacctcgtcc gggtcgacat cgatccgaag
     1141 tcgatacggc tgcaaaaaga ggaagcgatc tcctaccggt gggaggatga agcaggtctt
     1201 caagtcctcc atgaagaaca gctcctggcg tttccgatcg cctttgaatt tgaaccgttc
     1261 aggaccgctc ttcgccaagc agcgttgtga tgcactcccg gacgatcacg tgcgccttct
     1321 tgtcgggctg agtcacgtac caggaacccg agcgcggaca gccttcacac cccagaccca
     1381 gcgaatcgta tcggaaacag gatcggctga tgaacaacgg ggcttggaac gccggatcga
     1441 cgaacgtcgg ttcgaacggc cacaatgcgg tatccccttc aagccgttca agccaatggt
     1501 agccgccgat gaggttcagc cgggtttcac cgatcaagcg ggcgctttga ctgttggcga
     1561 ggtagaggta gatgtcacag aagaccggca gccgccgctt ctcgaagaac cggatgtggg
     1621 cgatgttgtt gagcccgaac cgaatccgct caaggcaccc gtcatcgctc gcccgatcga
     1681 taagccaagt gagatcctca aagaaccgtt cttcaagaaa ggtgatcgga gcgagcggaa
     1741 agtagagaaa cgacccgtcg gtgaagagca gttcttccaa cggtttccct ttgccaacct
     1801 gctttctcag atgggtgggg tccagatagg ggaggcgatc cccgtcgacc aacaaacttc
     1861 gtttgggaag ctgctcccat gctttcttct cgacatcgcc gagatcaagc ggttgatcga
     1921 cgtagcgctt gaagcgttga tcgaggcttc tgtagaaggt ccggcggatc ttcttaagcg
     1981 tcgagagcgg caaaaagatc tcttcaattt tagtgaactc ccattcacca cgttcaatgt
     2041 tcccagagtg aagagggatt ggtcgctttg ggaaaagatc gtcgcaagat tctctacgac
     2101 gtcttggttt cggtgtgcct tctgaagtga gatatgggct tgggcggtgc ccgccttgct
     2161 ttgaatggtg agcttcgaat cttccagaat gacggtgagg tcgatcggtt tctgataggg
     2221 gggaatctct tcggaaatga gagggacgtt cagattgtgg ccgctgacca gatggagttc
     2281 cgagccgatc gtcggccttt ccgcatcgtt cgggagagga atccaaatcc gcgttcccgc
     2341 ctcggcaaag ccgacacctt tgacggcaaa gcgaaccggc tcaaacagtc cgagacggtt
     2401 ttccgcaagg
//
LOCUS       DAOPUZ010000182         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_46398_length_2409_cov_2.920136, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000182 DAOPUZ010000000
VERSION     DAOPUZ010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46398_length_2409_cov_2.920136"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKN79_03780"
     CDS             <1..437
                     /locus_tag="PKN79_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ94916.1"
                     /translation="NAKREEFREILKQIGLESRLKDNVGLLSGGQRQALTLLMTAMSR
                     PKMLLLDEHTAALDPRNAQIVMDLTERFISEQKLTALMVTHNMQFAIDFGTRLIMMDE
                     GQIILDLSGEEKKDLTVDKLVRLFKDIRKKTYATDAALLTTD"
     gene            569..1852
                     /locus_tag="PKN79_03785"
     CDS             569..1852
                     /locus_tag="PKN79_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ94917.1"
                     /translation="MLEQLLNVSIGMADILMVTTVGEAAVSAISLVDSITILIVTLFG
                     AFATGGAVVASQYLGKKDDESANTAAGQLLNLTVLIATLLLLLCMPIRRGIISTIFGK
                     IEPRLLDYGSTYFVYILLSLPFLAAYNSGAALFRSMGNSRVSLYISVVMNILNIGGNA
                     FFIFVMKMGVAGAGLSTLISRIIGSILVLGLLRNPRNRIAIRSYNIFHIKGWMIRKIL
                     FVGIPTGVEGSLFQVGKLLVQGFIASFGTAPLAANAIASSVSTFINIPGGAISMASVT
                     VIGQTIGARRTDLSVFYAKRLLLFAYIAMFILATPIFFLAPTIASFFNLSEEATKIAT
                     VLFQSSMVVSVFFWSSSFVLPNFLRAAGDAKFTMIVSIISMWAFRVGGSYIMAMNFGW
                     GVYGVWIGMYIDWVVRSIAFIRRFARGKWKEIQVI"
     gene            complement(1849..2181)
                     /gene="rsfS"
                     /locus_tag="PKN79_03790"
     CDS             complement(1849..2181)
                     /gene="rsfS"
                     /locus_tag="PKN79_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNZ94918.1"
                     /translation="MNERTLSDAQAIVSYLEDRRCSDVHMIDLEGRASWTDCFIIATV
                     NSVGHLRGVVHNIWDLFNERGIAVANRQKKPAGDGWELIDCGDIIVHLMSEELREFYA
                     LERLWREL"
     gene            complement(2178..>2409)
                     /locus_tag="PKN79_03795"
     CDS             complement(2178..>2409)
                     /locus_tag="PKN79_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94919.1"
                     /translation="SSDPASALLENRKALRKSPLVDTLAVIGEDSRIAARLRRIDTTT
                     FLDLRGIPIEEDVLDVYLSDVRTYQMRRKTTQ"
BASE COUNT          523 a          666 c          625 g          595 t
ORIGIN      
        1 acaatgcaaa acgggaagaa tttcgcgaaa tcctcaaaca aatcggattg gaaagccggc
       61 tgaaagacaa cgtcggtctt ctctccggcg gccagcgcca agccctcacc ttgctgatga
      121 cggcgatgag tcggccgaag atgctgctcc tcgatgagca caccgcagcc cttgatccga
      181 gaaacgccca aatcgtgatg gatctgacgg aacgcttcat cagcgagcag aaactcactg
      241 cgttgatggt aacgcataac atgcagttcg cgatcgattt cggaacccgg ctcatcatga
      301 tggacgaagg ccagatcatc ctggatctca gcggagagga gaagaaagac cttaccgtcg
      361 acaagttggt tcgcctcttt aaagatatcc gaaaaaaaac ctatgccacg gacgccgccc
      421 tgctgacaac cgactaaacg gtgcttgttc gtttgatgaa aacagcatag gatgggtgtg
      481 aggatacatc ttgcacaaca ggagccgctc acttgagcat gggcaatttt ttgaatcggg
      541 attcctcttc aagctgatca tcccactgat gctggaacag ctcttgaacg tctccatcgg
      601 gatggcggat attctcatgg tcacaaccgt cggtgaagcg gcggtcagcg cgatcagtct
      661 ggtcgattcg atcacgattc tcattgttac gctttttgga gcctttgcaa ccggaggggc
      721 ggtcgtcgcc agccagtacc tcggaaaaaa ggatgacgaa tcggcgaata ccgccgccgg
      781 gcagctcttg aatctcaccg ttctcatcgc tacccttctt ctgctattgt gcatgcccat
      841 cagacgtgga atcatcagca cgatcttcgg gaagatcgaa ccccggctgc tcgattacgg
      901 ctcgacctat tttgtgtata ttcttctctc cctgccgttt ctcgccgcgt acaacagtgg
      961 agcagccctc tttcggtcga tgggcaacag ccgggtgtcc ctgtatatca gcgtcgtcat
     1021 gaatatcctg aatattggcg gaaacgcttt tttcatcttt gtcatgaaga tgggggttgc
     1081 cggagcgggc ctttccaccc tcatcagtcg aatcatcggc tcgattctgg tgttgggtct
     1141 gttgagaaac cccagaaacc gcatcgcaat ccgctcctac aacatcttcc acatcaaggg
     1201 ttggatgatt cgaaaaatcc tgttcgtagg gatccccacc ggggtggaag gctctctctt
     1261 ccaagtcgga aagctgctgg ttcaggggtt catcgcttcc ttcggaaccg cgccgcttgc
     1321 ggcgaatgcg atcgcctcaa gcgtctccac gttcatcaac attcccggcg gggcgatttc
     1381 gatggcctcc gtcacggtca tcggtcagac gatcggggct cgcaggaccg acctctcggt
     1441 cttctatgcc aaacggcttc tcctgtttgc atatatcgcg atgttcattc ttgcgacgcc
     1501 gatcttcttc ctcgccccca cgatcgcttc gtttttcaat ctcagtgaag aagcgaccaa
     1561 gatcgcgacc gttctcttcc aatcatcgat ggtcgtctcc gtcttcttct ggtcctcctc
     1621 tttcgttctt ccgaactttc ttcgggctgc gggggacgcc aagttcacga tgatcgtttc
     1681 aatcatcagc atgtgggctt tccgggtcgg aggctcgtac atcatggcga tgaacttcgg
     1741 atggggagtc tatggggtat ggattggaat gtacatcgat tgggtcgtcc gctcaatcgc
     1801 cttcatccgg cggttcgccc ggggaaaatg gaaagagatc caagtgattt aaagttcccg
     1861 ccacagccgt tcaagagcgt agaactccct gagttcctca ctcatcagat gaacaatgat
     1921 atccccgcag tcgatcaatt cccacccgtc tccggcgggt ttcttctggc ggttggcaac
     1981 ggcgatgccc cgctcgttga aaagatccca gatattgtgg actactcccc gaaggtgtcc
     2041 gacgctgttg accgtggcga tgatgaagca atcggtccag gaggctcgac cctcaaggtc
     2101 gatcatatgg acatcactgc agcggcggtc ttcaagatac gaaacaattg cttgagcatc
     2161 gcttagggtt cgttcattca ttgtgtagtt tttctcctca tctgataggt gcgaacatcg
     2221 gaaagatata catccaatac atcttcttcg atcggtattc ctcgaagatc gaggaaggtc
     2281 gtcgtgtcga tccgcctcag gcgggcggcg atgcgcgaat cttcgcctat cacggccagt
     2341 gtatcgacca ggggcgattt tctcaaggct ttcctgttct cgaggagcgc agaggccgga
     2401 tcggaactt
//
LOCUS       DAOPUZ010000183         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_47530_length_2369_cov_3.009939, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000183 DAOPUZ010000000
VERSION     DAOPUZ010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47530_length_2369_cov_3.009939"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PKN79_03800"
     CDS             complement(<1..960)
                     /locus_tag="PKN79_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HNZ94920.1"
                     /translation="MDTFKEYEFWFVVGSQFLYGPETLEQVALHARTMTDGLNKKGLL
                     PTNVIYKSTVKSADEITQVIKEANASDRCAGLIVWMHTFSPSKMWINGLALLQKPYVH
                     LHTQFNRMIPNEEIDMDFMNLNQSAHGDREHGFIAARMHRKRKIVVGYWEDEDVQRSI
                     ASWMRSAVGAAESRNLKLVRFGDNMREVAVTEGDKVEAQIKFGWQVNTFPVSELVEQI
                     KTVSEEAIDKRFEELLELYHLNTDDEDSVRYQVQVELALRSFLKEKGAKAFSNTFEDL
                     SGMKQLPGLATQHLMGTGYGYGGEGDWKVSAMTRIVKAMSEGLE"
     gene            complement(977..1954)
                     /locus_tag="PKN79_03805"
     CDS             complement(977..1954)
                     /locus_tag="PKN79_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ94921.1"
                     /translation="MTTLRKKAETDEQSLHRWRRIEEVFRQLMPFIGFVGLVLVFSIL
                     TKGRILNPRNISLMLSQVYVLMIASLGVFMIMTVGGLDFSQGSILGLTSIVFAWVSPH
                     GIVLGIVAAMLAGALMGAFNGFFHVKFKIASFIVTICTMYLFRGLCEYLTTHGPISAS
                     LKVLSLDETWLKILLTTIVITIGFLVIHFTRLGYDLRAIGAGEIAARFSGSRPELTTF
                     LVYTFAGMITGFASFINVIRVGSITGTAGSQLETQILIALVLGGMPIGGGAKARFSNI
                     IFGSLTYSVLERSLPMVFPVSATQQLVKGIIFLIVVALTIDRKSLRVIK"
     gene            complement(1967..>2369)
                     /locus_tag="PKN79_03810"
     CDS             complement(1967..>2369)
                     /locus_tag="PKN79_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ94922.1"
                     /translation="AKKRVLKKKELHAFAQSHADRMSVRMTGIKQYVFDLSGGNKQKV
                     VLARWLGRDSDIFVLDSPTRGIDVKVKADIYALMSELKERGKSIIMISEEILELIGMS
                     DRILIMKNGKFNGTFTRSVALTEEDLIAKMV"
BASE COUNT          574 a          671 c          553 g          571 t
ORIGIN      
        1 ttccaagcct tcactcatcg ctttcacgat gcgggtcatc gccgagactt tccaatcccc
       61 ttcaccacca tacccatagc cggtacccat caggtgttga gtcgccaaac cgggaagctg
      121 cttcatcccg ctgaggtcct caaaggtgtt gctgaacgcc tttgcgccct tctctttcag
      181 gaatgaacga agggcgagtt caacctgcac ttgatagcga accgagtctt cgtcatcggt
      241 gttgaggtga tacagctcca gaagctcctc aaagcgcttg tcgatcgcct cttcggagac
      301 ggtcttgatc tgctcaacaa gctcactgac gggaaacgta ttcacctgcc agccaaactt
      361 gatctgcgct tcgactttgt caccttcggt tacggccact tcgcgcatat tgtctccgaa
      421 gcggaccagc ttcagattcc ggctctccgc cgctccgacc gccgagcgca tccaggaagc
      481 gatcgagcgc tgaacatcct catcctccca atacccgacg acgatttttc gcttccggtg
      541 cattcgggct gcgatgaacc cgtgctcgcg gtctccatgg gcgctttggt tgaggttcat
      601 gaaatccata tcgatctctt cgttgggaat cattcgattg aactgggtat gaaggtgcac
      661 atacggtttt tgcagaagcg caagcccgtt gatccacatc tttgagggac tgaacgtgtg
      721 catccataca atcaagccgg cacatcggtc gcttgcattc gcctctttta tcacctgagt
      781 gatctcgtcg gcgctcttga ctgtcgactt atagatcacg ttcgtcggca acagtccttt
      841 tttattcaaa ccgtcagtca tcgtccgagc gtgaagagca acctgctcaa gggtctcggg
      901 accatagaga aactgggaac ccaccacaaa ccaaaactca tactctttga atgtgtccat
      961 acactcctcc aaccaattat ttaattaccc tgagagactt tctatcgatc gtcaaggcga
     1021 caacgatcag gaagatgatt cctttcacca actgctgggt agcggaaacg gggaacacca
     1081 tcggcaaact cctttccaga acggagtacg tcagtgatcc gaagatgata tttgaaaagc
     1141 gggcttttgc acctccgccg atcggcatgc ccccgagaac gagggcgatg aggatttgcg
     1201 tttccaattg gctaccggcc gtaccggtga tcgatcctac tctaatgacg ttgatgaagg
     1261 aagcaaaacc ggtgatcatt ccggcaaacg tgtaaaccag gaaggtcgtc agctcggggc
     1321 gggagccgga gaaacgggcg gcgatctccc ctgctccaat cgctcgcaga tcgtagccga
     1381 gccgggtaaa gtgaatcacc agaaacccga tcgtgatgac gatcgtcgtc aacaggattt
     1441 tcagccacgt ctcatcaagc gaaaggactt tgaggctggc ggaaatcgga ccgtgagtcg
     1501 tcaaatactc acacaatccc ctgaagaggt acatcgtaca aatcgtaacg atgaaggaag
     1561 caattttaaa ctttacatgg aagaacccgt taaaagcacc catcagcgct ccggcgagca
     1621 tcgccgctac aatgccaaga acaattccat gcgggcttac ccaggcgaag acgatcgagg
     1681 tgagtccaag gatcgatcct tgggagaaat ccaaccctcc caccgtcatg atcatgaata
     1741 ctccaaggct tgcaatcatc aaaacatata cctgactgag catcaggctg atgtttctcg
     1801 ggttcaggat tcgtcctttg gtaagaatcg agaacacaag caccaagccg acaaagccga
     1861 tgaacggcat cagttgccgg aatacctcct caattcttcg ccatcggtgg agagattgct
     1921 cgtctgtttc agctttctta cgtagagtcg tcattatcta tcccccctat accatcttgg
     1981 cgatcagatc ttcttcggtc agtgctacgg aacgggtaaa tgttccgttg aacttcccgt
     2041 ttttcatgat gaggatccga tcactcattc cgatcagctc aagaatctcc tcactgatca
     2101 tgatgatcga cttcccccgt tccttcagct cgctcatcag tgcgtaaata tccgctttta
     2161 ccttgacgtc gatgcctcga gtcgggctgt caagaacaaa gatatcggaa tcacggccaa
     2221 gccagcgggc caagaccacc ttctgcttgt ttcccccgga aaggtcgaag acatactgtt
     2281 tgatcccggt cattcggaca ctcatccgat cggcatggct ctgggcaaat gcatgtaatt
     2341 ccttcttttt caacactcgc ttttttgca
//
LOCUS       DAOPUZ010000184         2368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_47554_length_2368_cov_3.699957, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000184 DAOPUZ010000000
VERSION     DAOPUZ010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47554_length_2368_cov_3.699957"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PKN79_03815"
     CDS             <1..399
                     /locus_tag="PKN79_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HNZ94923.1"
                     /translation="LLFCSGAMTVLGSIQAGTVGDYRLIMIKSIMDGAMAVIFASVYG
                     PGVIASALFVLVYQGFFTLAGGWIEPILGERGIGELGAVGGVLLLMIGFNLLNIRKTK
                     TGNFLPAMILAPIFAILTPSVAALFSSLGF"
     gene            complement(356..736)
                     /locus_tag="PKN79_03820"
     CDS             complement(356..736)
                     /locus_tag="PKN79_03820"
                     /inference="COORDINATES: protein motif:HMM:NF018697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ94924.1"
                     /translation="MESFWNRVANLLNTSTVVELAQILDVKRSTLSSWIHSDRRPPME
                     VLLKITRLTGVDIHRLERGFSYSLEEDDEVAEHHTSIKRFELIEFINSLDDEELEAVW
                     AFLRLCERRTVKTRGSKTVQQPKE"
     gene            complement(889..>2368)
                     /gene="glgP"
                     /locus_tag="PKN79_03825"
     CDS             complement(889..>2368)
                     /gene="glgP"
                     /locus_tag="PKN79_03825"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HNZ94925.1"
                     /translation="SWEKAWEITVSSLGYTNHTLMPEALEKWSVPMMGSLLPRHLQII
                     YEINYRFIQEAVSHFPLQPQILNQISIIEESNPKHVRMANLAIVGSHSTNGVAALHSQ
                     LLRDSMFPHFNTIFPDRFNNKTNGITQRRWLLASNPELSKLITKAIGSGWITDFSRMA
                     KLKKFADDSSFVADFAKIKAANKVRASNFLRTDTSIILNPNTFFDVQVKRIHEYKRQL
                     LNVLNILYIYNDLKQGGEETRDMEPTTFLFGGKAAPGYVNAKLIIKLINNIAQVINAD
                     QEVNDRLKVHFLPNYRVTMAEALIPATNLSQQISTAGTEASGTGNMKFMINGALTIGT
                     LDGANIEIAEEAGAENLFIFGNTEEQIEALRPSYDPHSYIRSNPKIRAIVDLLQSGYF
                     NVNEPGIFDPLYRSLLKEGDRYFLFADLQMYADAHRKARELYILDQTEWNRKAVLNVA
                     SGSKFSSDRTISEYAEEIWKIKSLPVKQNGHDDTVLEDARKR"
BASE COUNT          462 a          604 c          719 g          583 t
ORIGIN      
        1 ctgctgttct gttcgggagc gatgacggtc ctcggctcga ttcaggcggg aaccgtcggc
       61 gactatcgcc tgatcatgat caaatcgatc atggacgggg cgatggcggt gattttcgct
      121 tccgtctacg gaccgggagt gatcgccagc gcgctcttcg tgcttgtcta tcaggggttc
      181 ttcacccttg ccggagggtg gatcgaaccg atcctcggcg agcggggaat cggggagctc
      241 ggggcggtcg ggggagttct gctgctgatg atcgggttca acctgttgaa catccgcaag
      301 acgaaaaccg gaaactttct tccggctatg attctggcgc cgatcttcgc gatcctcact
      361 ccttcggttg ctgcactgtt ttcgagcctc gggttttaac cgttcggcgc tcacagaggc
      421 gcaggaatgc ccaaaccgct tcaagctctt catcatcgag ggagttgatg aactcgatca
      481 gttcgaaccg tttgatcgag gtgtgatgct cggcgacctc gtcatcctcc tcgagcgagt
      541 aggagaagcc ccgttcaagc cggtggatat ccactccggt cagtctggtg atcttcaaca
      601 gaacctccat gggaggccgg cggtcgctgt ggatccagct ggagagggtc gagcgcttca
      661 catcgaggat ttgggcaagc tctacgacgg tggaggtgtt aagcaggttt gcaacgcggt
      721 tccagaacga ttccatgccg taaatatagc agccgattgc cctgaatgac gaaaacccgg
      781 caggtatata atcctttatg agattcttct tatagggcaa tcaattaatg aaaccgacgg
      841 tgacgaaaac aagtcaccct gacgagaagg tgacttgttt gacggtcatc aacgctttcg
      901 ggcatcttca aggactgtgt catcatgccc gttctgcttt accggcaagc tcttaatctt
      961 ccagatctcc tcggcgtatt cgctgatcgt ccgatcgctt gagaactttg aaccgctggc
     1021 gacattgagg actgccttgc ggttccattc cgtctgatcg aggatgtaca gctcgcgggc
     1081 ttttcgatgg gcatcggcgt acatctgcag atcggcgaag aggaagtagc gatctccctc
     1141 cttcagcaag gagcgataga ggggatcgaa gattcccggc tcattgacgt tgaagtagcc
     1201 gctttgcagc aaatcgacga ttgctcggat cttgggattg ctcctgatat aggagtgggg
     1261 atcgtaggaa gggcgaagcg cttcgatctg ctcttcggtg tttccgaaga tgaagaggtt
     1321 ttccgctccc gcttcttcgg cgatttcgat gttcgctccg tccaacgtgc cgatcgtcaa
     1381 cgctccgttg atcatgaact tcatgttgcc cgttcccgac gcttcggtac ccgccgttga
     1441 aatctgctgg ctcaggttgg tcgcaggaat caacgcttcg gccatcgtaa cacggtagtt
     1501 tggcagaaag tgaactttaa gccggtcgtt gacttcctgg tcggcgttga tgacctgggc
     1561 aatgttgttg atcagcttga tgatcagttt cgcattgacg tatcccggag ccgcttttcc
     1621 gccgaagagg aaggtggtgg gctccatgtc cctggtctct tcaccgcctt gcttgaggtc
     1681 gttgtagatg tagaggatgt tcagcacgtt gagcagctgg cgcttgtact cgtggatccg
     1741 cttcacctgg acgtcgaaga acgtgttggg gttcaggatg atcgaggtgt cggtgcgaag
     1801 gaagttggaa gcccgcacct tgttcgccgc tttgatcttg gcgaaatcgg caacgaacga
     1861 ggaatcgtcg gcaaacttct tcagcttcgc catccgggag aagtcggtga tccatccgct
     1921 tccgatcgct ttggtgatca gcttgctcag ctcgggattc gaggcgagca accagcggcg
     1981 ctgggtgatc ccgttggtct tgttgttgaa ccggtcgggg aagatcgtgt tgaagtgggg
     2041 gaacatcgaa tcacggagca gctggctgtg gagggcggcg accccgttgg tggagtggct
     2101 gccgacgatc gccaggttgg ccatccgcac atgcttcgga ttggattcct cgatgatcga
     2161 gatctgattg agaatctgcg gctggagcgg gaagtggctc accgcttcct ggatgaagcg
     2221 atagttgatc tcatagatga tctgaaggtg acggggcaga agcgagccca tcatcgggac
     2281 gctccacttc tccagcgcct cgggcatcag ggtgtggttg gtgtagccaa ggctggaaac
     2341 ggtgatctcc cacgccttct cccaggaa
//
LOCUS       DAOPUZ010000185         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_47634_length_2366_cov_2.542190, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000185 DAOPUZ010000000
VERSION     DAOPUZ010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47634_length_2366_cov_2.542190"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PKN79_03830"
     CDS             <1..803
                     /locus_tag="PKN79_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94926.1"
                     /translation="GYWKNVPAGRNHSIMPNDYMVRGVQIDPAVHDSYFPNPEDGDVN
                     IKSSRILISAISEGVFRFDAAFGHTSGEEFIREKRQFLGKRSEQPRFLYTHVDRPGHT
                     TDIGVLADNETELWEERLRIANGELEDDLAVVLEHNPDALIIVAADHGPYLTKNGKDL
                     NVPTYSLGDITRYDVQDRYGTLLAIRWPEKGYETRYDIRILQDVLPAVFAYLYDDEAL
                     FDRLRMERKTLYPYVTGGVVVEDGIVIGGADDGRPLFDRVGIRVLKDR"
     gene            873..>2366
                     /locus_tag="PKN79_03835"
     CDS             873..>2366
                     /locus_tag="PKN79_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ94927.1"
                     /translation="MQAIILAAGMGKRLKELTKDNTKCMVEVNGITLIERVLRQLDRL
                     GLSKIVLVVGYKGDKLISFVDTLSINTPIVFVDNPIYDRTNNIYSLYLAKEYLLKEDT
                     LLLESDLIIEDGILEKLAAFSSPNAVLVAPWEPWMDGTVVTLDCQNRIQTFLDKRQFR
                     FSDTDTYYKTVNIYRFSREFLTSHYVPFLEAYCTALGHNEYYEQVLKVIAQLSEPWMH
                     ALVLEDEKWYEIDDIQDLDIAEILFETDPEKRFARINARYGGFWRFGKIVDFCYLVNP
                     YFPPQKLVDELQKSFELLLRQYPSGQTVNNLLAANYYGLRVPSVMVGNGAAELIKGLM
                     ETEEGKIGLTAPSFDEYRNRAKEEQLLVFTPSNPDFSYQVEDIVSFYAGKNLSMLLLV
                     NPENPTGFCLTNEALNRMIQWSKKERIVLVVDESFGDFSETETLFDQQLLDKNPHLVV
                     IKSISKSFGVPGLRLGVLATTNEKIMKRISKEMSIWNINSFGEYFMQI"
BASE COUNT          578 a          634 c          643 g          511 t
ORIGIN      
        1 ctgggtactg gaagaatgtc ccagcaggcc gcaaccattc gatcatgccg aacgactaca
       61 tggtcagggg agtccaaatc gaccccgccg tgcatgactc ctacttcccc aatccggaag
      121 acggcgatgt gaatatcaag agctcaagga tcctcatctc ggcgatctcg gagggagtct
      181 tccgctttga tgcagcgttc gggcatacga gcggggaaga attcatcagg gagaagcggc
      241 agttcctcgg taagcgatcg gaacagccgc ggtttctcta tacccatgtc gatcggcccg
      301 gacacaccac cgacatcgga gtgttggccg acaatgagac cgaactgtgg gaggaacgcc
      361 tgcgcatcgc gaatggtgaa ctggaggacg atcttgcggt cgtgctcgag cacaatccgg
      421 atgccctcat catcgtggcc gccgatcatg ggccgtatct gaccaagaac ggcaaagacc
      481 tcaacgttcc gacatattcg cttggtgaca tcacccggta cgatgttcaa gaccgatatg
      541 gaacgcttct ggcgattcgt tggccggaga aagggtatga aaccaggtac gacatcagga
      601 ttcttcagga tgtccttccc gccgtgttcg cctatctcta tgatgacgag gcgttgttcg
      661 accggctccg gatggaacga aagacgctct atccgtacgt gacgggagga gtcgtggtcg
      721 aagacggcat cgtgatcggg ggcgcggatg acggaagacc gttgttcgac cgggttggaa
      781 tccgagtcct gaaagatagg tgaggggatc gagaccggtc gtcttttgaa caggtgtatg
      841 atagggtatg agcacatgga aggagaaaag agatgcaggc gataatcttg gcggccggaa
      901 tgggaaagcg attgaaggaa ctgacgaaag acaatacgaa atgcatggtc gaagtcaacg
      961 gaatcaccct catcgaacgg gttctcagac agcttgaccg gcttggtttg agtaaaatag
     1021 tcctggtggt gggatacaag ggagacaagc tcatctcctt tgtcgataca ttgtcaatca
     1081 acacacccat cgtattcgtc gacaacccga tctatgacag gaccaacaac atctactccc
     1141 tctatctggc caaagaatac ctgcttaagg aggataccct ccttcttgaa tcggatctga
     1201 tcatcgagga cggcatcctg gagaagctcg cggctttctc ttctccgaat gccgttctgg
     1261 tcgctccttg ggagccttgg atggacggaa cggtcgtcac cctcgattgc cagaacagaa
     1321 tccagacgtt tctcgacaag cggcagttcc gattcagcga tactgacacc tactataaga
     1381 cggtgaacat ctaccgcttc agcagagagt tcctgacttc tcattacgtt ccctttctcg
     1441 aagcctactg caccgccctt gggcacaacg aatactacga gcaggtcctc aaggtcatcg
     1501 cccagctgtc cgaaccatgg atgcacgccc tcgtcctgga agacgagaag tggtacgaga
     1561 tcgacgacat ccaggatctg gacatcgccg agatcctctt tgaaaccgac ccggagaagc
     1621 ggtttgcgcg gatcaacgcc cgctatggcg gattttggcg cttcgggaag atcgttgatt
     1681 tctgctacct cgtgaacccg tatttcccgc cccaaaagtt ggtagacgag ctccagaaga
     1741 gctttgaact cctgcttcgc caatatccct cgggacagac ggtcaacaat cttttggcgg
     1801 cgaactacta cggtctccgg gttccctcgg tgatggtggg caacggggcc gccgaactca
     1861 tcaaagggct catggagacg gaagaaggga agatcggatt gacggccccc tccttcgacg
     1921 agtaccggaa ccgggcgaag gaggaacagc tcttggtgtt cactccttcc aatcccgatt
     1981 tctcctatca ggttgaggat attgtctcct tctacgccgg caagaacctt tccatgctgc
     2041 ttctggtcaa tccggagaac cccaccggct tctgtctcac caatgaagca ttgaaccgca
     2101 tgatccaatg gagcaaaaaa gaacggatcg tgctggtcgt cgacgagtcg tttggcgact
     2161 tctccgaaac ggagacgctg ttcgaccagc agcttcttga caaaaacccc catcttgtcg
     2221 tcatcaaatc gatctccaaa tccttcgggg ttccgggact ccgcctcggg gtgcttgcga
     2281 cgacaaatga gaagatcatg aaaagaatct ccaaagagat gtcgatctgg aatatcaact
     2341 cattcggaga atacttcatg cagatt
//
LOCUS       DAOPUZ010000186         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_48273_length_2344_cov_3.107471, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000186 DAOPUZ010000000
VERSION     DAOPUZ010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48273_length_2344_cov_3.107471"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..2277
                     /locus_tag="PKN79_03840"
     CDS             199..2277
                     /locus_tag="PKN79_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNZ94928.1"
                     /translation="MSVKSTDLRNVAIVGHNETGKTTLVEQMLAYAGVISRAETIESG
                     KTTSDYAEAEIANKISIYATLQSMTYGDKTFNIVDTPGTAGFIGETISGFRSTESAIM
                     VVDGRVGPQIETIKLWRNLDDRNKPRAVFINKMDRDHADFHKVLDDLKESFNVTFVPV
                     VIPVGASSEYKGVVNLIENKVYYYQPNGKETSGDIPAELEELVEEYRTELIESAAEGS
                     DELIEKYFEAGTLEPDEIRQGLQLGLRGNRLVPIFAGASGLGSGIIPLMNFIQNNFPD
                     PVGATDWSIDGEGNTHEFPITEEGPGSALVFKTTIDQFSGKLSFIKVLRGTIKGDTDL
                     TNPATGKRERPGKVYRMVGKKLNEVGSLVAGDVGVIAKSGITATNTSLVEGGDGSLIF
                     KPIAFPQPIYALAISSDDKKAEVKMNEALNRIAEEDLTFTIGFNEETKENVVAGQGEL
                     HLNMILDRVRDRQKVTINTKLPKVAYRETISKTSPICEYTHKKQSGGHGQYARVLIDI
                     KPLERGQYYSFENVIKGGAVSKGYIPGIEKGLHEQMEEGFLAGYPMMDIGITLHDGKE
                     HPVDSSEMAFKLAAKGAMRLALEKAGATLLEPIVSLSVFIDEEYLGDILSDLSGKRGR
                     VLGQEDIGHLILVKALVPQSELLNYAIDLKSMTSGTGSFEMEFDHYEPLSGKLADDVI
                     RAYKESQEEA"
BASE COUNT          630 a          589 c          652 g          473 t
ORIGIN      
        1 cagagagaag aaatccaaga ggttattaca tcaatccgat ttctattata tagtatggac
       61 catcgaattg tttgtagtgt aggatactac acgcaactct atgcaagagc aaaagaaacc
      121 ttttgacagg tgccaccaaa ggagcgtacc ataccggtat gcgttttgga ttatcaattg
      181 gtgatcgaag gaggatctat gagtgttaaa agcacggact tgaggaatgt cgccattgtt
      241 ggccacaatg aaaccgggaa aacaaccctc gttgagcaaa tgcttgccta tgccggggtt
      301 atttcccggg cagaaacgat tgaaagtgga aagaccacca gtgactacgc agaggcggag
      361 atcgccaaca agatttctat ctatgcaacg ctccagtcga tgacctacgg ggacaagacc
      421 ttcaacatcg tcgacacccc gggaacagcc ggcttcatag gcgaaacgat cagtggattc
      481 cgatccaccg aatcagcgat catggttgtc gatggccgcg tcggtccgca gatcgagacg
      541 atcaagcttt ggcgcaattt ggatgaccga aacaagccgc gggcggtgtt catcaataaa
      601 atggatcggg accacgccga ttttcataaa gttcttgatg acttgaagga gtccttcaac
      661 gtcaccttcg ttccggtagt gattccggtc ggagccagca gtgaatacaa aggggtcgtg
      721 aacctcattg aaaataaagt ctattactac caaccaaacg gaaaagagac aagcggcgac
      781 atccccgccg agctggaaga gttggttgaa gagtatcgca ccgagctgat tgaatccgcc
      841 gctgaaggtt cggatgagct gatcgaaaag tactttgaag cgggaacgct tgaaccggat
      901 gaaattcgtc aaggcttgca gcttgggttg agaggaaacc ggctggttcc gatcttcgcc
      961 ggcgcctcgg gcctcggttc ggggatcatt ccgctgatga acttcatcca gaacaacttc
     1021 cccgacccgg tgggagcaac cgactggagc atcgacggcg aaggaaacac ccacgaattt
     1081 cccatcaccg aagagggacc gggctcggcg ctcgtcttca agacgacgat tgaccagttc
     1141 agcggaaagc tttccttcat caaggtgctt cggggaacga tcaagggtga taccgacctc
     1201 accaacccgg cgaccggcaa gcgcgaacgc cccggcaaag tgtatcgcat ggtcggaaag
     1261 aagctgaatg aagtcggttc cctggtcgcc ggagatgtcg gagtaatcgc caagagcggc
     1321 atcaccgcaa ccaatacctc gctggtggag ggcggagacg gatcgctcat cttcaagccg
     1381 atcgccttcc cgcagccgat ctatgcgctt gcgatctcct ccgatgacaa gaaagcggaa
     1441 gtgaagatga acgaagcgtt gaacaggatc gccgaggaag acctcacgtt cacgatcggg
     1501 ttcaatgaag agacgaagga aaacgtcgtt gccggccagg gtgaattgca cttgaacatg
     1561 atccttgatc gagtgcggga tcggcagaag gtcacgatca acacgaagct tccgaaagtc
     1621 gcctatcggg agacgatcag caagacgagt ccgatttgtg aatacaccca caaaaagcag
     1681 agcggcggcc acggtcagta cgcccgagtc ctcatcgata tcaagccgct tgagcgggga
     1741 caatactact cctttgagaa cgtcatcaaa ggcggcgccg tttccaaggg atatatcccc
     1801 ggaattgaaa aaggcctcca cgagcagatg gaagagggat tcctcgccgg ctatccgatg
     1861 atggatatcg gcatcaccct ccatgacggc aaggaacacc ccgtcgactc gagtgaaatg
     1921 gcgttcaagc tcgccgccaa aggcgcgatg agactggctt tggagaaagc gggagcgacc
     1981 ctgcttgaac cgatcgtttc cttgtcggtc ttcatcgacg aggagtatct tggagatatc
     2041 ctttccgacc tctccggcaa acgcggccgg gttctcggac aggaagatat cggtcacctg
     2101 attcttgtaa aggctttggt gccgcagagc gaactgctca actatgcgat cgacctgaag
     2161 tccatgacca gcgggaccgg aagttttgaa atggagtttg accactatga gccgctttcc
     2221 ggcaagttgg cagacgatgt gatccgtgcc tacaaggagt cccaggaaga ggcataagga
     2281 tcgagtatca gggacaatca gaagctcact acatttctga ttgcatgaga gtcaatttgt
     2341 agga
//
LOCUS       DAOPUZ010000187         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_48500_length_2336_cov_3.282771, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000187 DAOPUZ010000000
VERSION     DAOPUZ010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48500_length_2336_cov_3.282771"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PKN79_03845"
     CDS             <1..1084
                     /locus_tag="PKN79_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94929.1"
                     /translation="VPRVVLTDDRKAILKEYDQEEIDRAFLAEQAQAYAKKQPAKPKG
                     EAFEDTGSDLPSTYASKGYDPSLLALFEREEALFWYMSRERALGVFLVDVTPYGQARR
                     VRLLYQEGPRLDLRLIHDRLLIAMEERTLIEELLAQAVHILTDGTKAVLKIEGSKEIR
                     IAEKMERIESLDVYLMPTDISTVTVERSGHEPQKVAVRWEEGEVATIRPDLQRIVNEP
                     VTLLSNVGAVRYSSEGEAGESGMITLGSPSYPIALSVEKEGFVKSVLHIPHETGTVIR
                     FGVRKASVDVPDEQKRVYRRALGTILSFGSYVAAQALVAAYDPKHENPLWSLALGSTR
                     LLSVVSSVAMVAELSSYASAVGMMQE"
     gene            1096..1968
                     /gene="ftsY"
                     /locus_tag="PKN79_03850"
     CDS             1096..1968
                     /gene="ftsY"
                     /locus_tag="PKN79_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNZ94930.1"
                     /translation="MAKGFGARLKSLFGIKRFDESYFEELEELLIEGDLGSKLTMQIS
                     DEVRNLVRKKKDLTTRELQLLVKEQLRPMVHTFHPVIDRNALNVFLILGVNGVGKTTT
                     IAKIARLYKTQGYSVTLAAADTFRAAAIDQLEVHANRVGVRIVKQESGSDPGAVVFDA
                     ITSARARGDQLLLVDTAGRMHNKEHLVRELAKIDKIVQTKGIDSVHYKKFLVIDSTTG
                     QNGLSQATIFHQAIRLDALILSKYDSLAKGGALIQIGDSLGLPISFVGVGEKYGDLEL
                     FEAEPFLDSLVGLA"
     gene            1965..>2336
                     /locus_tag="PKN79_03855"
     CDS             1965..>2336
                     /locus_tag="PKN79_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94931.1"
                     /translation="MKQAVVIGVLLIMLSPLAGASMKANSIGQPLGGYEEGDRYRLEL
                     SSYSRVLYDEGKQVWVEIDQYIEPATVITTRTYRDGRIQKRTFTDGSLVKEQEGSTTS
                     TYTLDDAGRLIIASTAVREDEP"
BASE COUNT          541 a          583 c          721 g          491 t
ORIGIN      
        1 cgtgcctcgg gtcgtcctga cggatgatcg gaaagccatc ctgaaggagt atgatcagga
       61 ggagatcgat cgggcgtttt tggcggaaca agcccaagca tatgcaaaga agcaaccggc
      121 aaaaccgaaa ggggaggcgt ttgaggatac cggatcggat ctgccgagca cctatgcaag
      181 caaagggtac gatccttctc ttcttgcgct gtttgaacgg gaagaagccc tcttttggta
      241 tatgagccgg gaacgggccc tcggggtgtt tcttgtcgac gtgacgccgt atggtcaagc
      301 ccggcgcgtc cggctcttgt atcaggaagg acctcgcttg gaccttcgat tgatccatga
      361 ccgattgctg atcgccatgg aagagcgcac cctgattgag gagttgttgg ctcaggccgt
      421 acacattctc accgacggca cgaaggcggt tttgaagatc gagggatcga aggagatccg
      481 cattgccgag aagatggaga gaatcgaatc gttggatgtg tatctcatgc cgaccgacat
      541 atctaccgtt accgtggagc gaagcggcca tgaacctcag aaggtcgccg ttcggtggga
      601 ggagggggaa gtcgccacga tccgaccgga ccttcagcgg atcgtcaacg agccggtcac
      661 cctgctttcg aacgtcggag cggtacgcta ttcctcagag ggcgaggcgg gcgaatccgg
      721 catgataacc ttgggatcac cctcctatcc gatcgctctt tcagtggaaa aagagggatt
      781 tgtgaagagc gtcctccaca tccctcacga aacggggact gtcatccgct tcggagtgcg
      841 aaaagcttcg gtggatgtgc cggatgaaca aaagagagta tatcgtcgag cgctgggaac
      901 catcttgagt ttcggttctt atgttgctgc acaagcgttg gtggctgcat atgaccccaa
      961 gcatgagaac ccgctgtggt cattggccct tggttcgact cgcttgttga gcgttgtatc
     1021 aagcgtggcg atggtcgccg agctctcctc atacgcctcg gcagttggta tgatgcagga
     1081 ataaggaaga tagcgatggc aaaaggattc ggtgctcgat tgaagagctt gtttggaata
     1141 aagagattcg atgaatcata cttcgaagag ctggaagaac tcctcattga aggggatctg
     1201 ggctcaaagc tcacgatgca gatcagcgat gaggtgcgga atctggtcag gaagaagaaa
     1261 gacctgacca caagggagct gcagctgctg gtgaaggaac agcttcggcc gatggtccat
     1321 acgttccatc ctgtgatcga tcgaaacgcc ctcaatgtat ttctgatcct cggcgtgaac
     1381 ggggtcggca agacgacgac gatcgccaag atcgcccggc tgtataaaac gcaggggtat
     1441 tccgtcacgc tggcggcagc ggatacgttc cgggctgcag cgatcgacca gttggaggtt
     1501 catgccaatc gggtaggggt tcgaatcgtg aagcaggaga gcggcagcga tcccggcgcc
     1561 gtggtcttcg atgcgatcac aagcgcccgg gcccggggcg atcagctgct cctggtcgac
     1621 accgccggac ggatgcacaa caaggagcat ctggtccggg agctggcgaa aatcgacaag
     1681 atcgtgcaga cgaaggggat cgattccgtg cattacaaga aatttctcgt cattgattcc
     1741 accaccggtc agaacggcct cagtcaagca acgatctttc accaagcgat ccggttggat
     1801 gcgctgattc tctccaaata cgactctctg gccaagggcg gagctctgat ccaaatcgga
     1861 gattccttgg gtcttcccat ctcttttgtc ggagtcgggg agaagtatgg ggatctggaa
     1921 ctgtttgaag cggaaccgtt tctcgattct ctggtggggc ttgcatgaaa caggcggtgg
     1981 taatcggtgt tctgctcatc atgctgagcc cgcttgccgg ggccagcatg aaggcgaact
     2041 cgatcggcca gccgttggga ggatatgagg aaggcgatcg ctatcgcttg gaactctctt
     2101 cctattcgag ggtgctctat gacgagggca aacaggtatg ggtcgagatc gaccagtaca
     2161 tcgaaccggc aacggtgatc acaacccgga cctatcggga cggacgcatc caaaagcgaa
     2221 cgtttaccga tggctccctt gtgaaagaac aggaaggctc aacgacaagc acctacacgt
     2281 tggatgatgc cgggcggctc atcatcgctt cgacggccgt gcgggaggat gaacct
//
LOCUS       DAOPUZ010000188         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_48964_length_2321_cov_3.702118, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000188 DAOPUZ010000000
VERSION     DAOPUZ010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48964_length_2321_cov_3.702118"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /gene="aroF"
                     /locus_tag="PKN79_03860"
     CDS             complement(<1..1215)
                     /gene="aroF"
                     /locus_tag="PKN79_03860"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNZ94932.1"
                     /translation="MILILKKQITEAQKHAIREFLSDKGYTVKEIVGQEETVFGAVGQ
                     SSVDIRQVQLLEGVASVVPISKPYKLASREFKKEDTIVSVGKVKVGGNRIAIIAGPCA
                     VESRKQIMAVARSVHDAGAVILRGGAFKPRTSPYAFQGLGEEGLKYLREAGDAYGMPI
                     TSEIVSPSDVNMMAKYVDMFQIGARNMQNFELLKAVGKTKMPVLLKRGLSATIEEWLM
                     AAEYLMASGTDQVILCERGIRTYERATRNTLDISAVPVIQKLTHLPIIGDPSHATGMR
                     DMVGSMSLGLVASGASGLMVEVHTDPDKAFSDGPQSLYPSQFEKLMRDLDALSGVLGK
                     SMERLVKVEGPKTGGSEKKKTSSLVVAFQGERGAYSEMAIRRAFEEDTEVLPCPSFTE
                     LFNAVREGRALYG"
     gene            complement(1260..1997)
                     /locus_tag="PKN79_03865"
     CDS             complement(1260..1997)
                     /locus_tag="PKN79_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HNZ94933.1"
                     /translation="MPKIEICLDSVESIIASEQGGADRVEFCSDLFEGGLTPSLGMFK
                     VAKGRTAIPINVMIRPRGGDFCYSDLEFEAMKEDARLFKEAGANALVFGILTPEGKID
                     KKRSRELIAIARPLPVTFHRAFDMTSDPFEAIEDLIELGVERVLTSGGEASVLEGADL
                     LARLIKEYGNRIVIMPGAGITKRNFEKIHSIIKADEYHLYLHSEQPSKMVHRKDHVYM
                     GGLLRQEEYLVQHTDQSRVEAMIALVR"
     gene            2087..>2321
                     /locus_tag="PKN79_03870"
     CDS             2087..>2321
                     /locus_tag="PKN79_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNZ94934.1"
                     /translation="MDEHYWDSLFHRFRLALEKQGTILPSVSLVAQEHDDPFRILIAT
                     VISLRTKDAVTLKASRSLFSRAKTPSGILDLTEE"
BASE COUNT          454 a          721 c          622 g          524 t
ORIGIN      
        1 cccgtagagc gcccggcctt cccggaccgc gttgaagagc tcggtgaagg aggggcacgg
       61 aagcacttcc gtatcctctt cgaacgcccg tctgatcgcc atctcgctat atgctccccg
      121 ttcaccttgg aacgccacca ccaacgagga ggttttcttc ttttcactcc ccccggtctt
      181 cggcccttca actttgacca gccgctccat cgatttgccc aacacaccgc tgagggcgtc
      241 aagatctctc atcagctttt cgaactggga aggatacaac gattgcggac cgtcgctgaa
      301 cgccttgtcc gggtcggtat ggacctccac catcaggccg gaagctccgc tggctacaag
      361 gcccagactc atcgaaccga ccatgtcccg cattccggtc gcatggctgg gatcgccgat
      421 gatcgggagg tgggtcagct tctgaatcac cggcaccgct gagatgtcca acgtgtttct
      481 tgtcgcccgc tcgtaggtcc ggatcccccg ttcacagagg atgacctgat cggttccgct
      541 ggccatcaga tattcggcgg ccatcaacca ttcctcaatc gtggcggaga ggccccgctt
      601 gaggaggacc ggcatcttcg tcttccccac cgccttgagc agttcgaagt tctgcatgtt
      661 gcgggctccg atctggaaca tgtcgacata cttggccatc atattgacat cggagggact
      721 gacgatctcg ctggtgatcg gcatgccgta ggcatccccc gcttcccgga ggtacttcag
      781 gccctcttcc cccaggccct ggaacgcata gggactggtc cggggtttga acgccccgcc
      841 gcggaggatc accgccccgg cgtcgtggac gcttcgggcg accgccatga tctgctttcg
      901 tgattcgacc gcgcacggtc cggcgatgat cgcgatgcgg ttgccgccga ccttcacctt
      961 gccgaccgag acgatcgtgt cctctttttt gaactcccgg gaagcaagct tatatggctt
     1021 gctgatcggc accaccgagg cgacgccttc aagcagctgg acctggcgga tgtcgacgga
     1081 ggattggccg accgccccga agaccgtttc ctcttgaccg acgatctcct tgaccgtata
     1141 tcccttgtcg gagagaaact cgcgaatggc atgtttctga gcttcggtta tctgtttctt
     1201 gagaatgagt atcatgaaga aacgctagag ggaaaggggg aaaaaatcaa gagatgacgc
     1261 tagcgcacca gagcgatcat cgcttctacc cggctttggt cggtgtgttg aacaaggtac
     1321 tcctcctgac ggagcaatcc acccatgtag acatgatctt tccgatggac catctttgac
     1381 ggctgttcgc tgtgaagata cagatgatat tcatcggcct taatgattga atggatcttt
     1441 tcgaagttcc gcttcgtgat ccccgccccc ggcataatga cgatccggtt tccatactct
     1501 ttgatcaatc gggcaagcag gtccgctcct tcaaggaccg atgcttcccc gccgctggtg
     1561 aggacccgct ccactcccag ttcgatcaga tcctcgatcg cttcaaaagg gtcggaagtc
     1621 atgtcgaacg cccggtggaa ggttaccgga agcggccggg cgatggcgat caattccctg
     1681 cttcgtttct tgtcgatctt cccttcgggg gtgaggatgc cgaagacgag cgcattcgcc
     1741 cccgcttcct taaagagccg ggcatcctcc ttcattgctt cgaactccaa atcggagtag
     1801 cagaaatccc cgccgcgggg gcggatcatc acgttgatcg gaatcgctgt ccgcccctta
     1861 gcgaccttga acattcccaa ggagggagtg aggccccctt cgaagaggtc ggaacagaac
     1921 tccacccggt ccgctccgcc ctgttcggaa gcgatgatcg attcgacgga atccagacaa
     1981 atttctatct tgggcatcac aaactccttt cctgcagtgt accagcttgc atccccggtg
     2041 ggtagggcac cttgtcattt gctccgacct gtggtagctg atacctatgg acgaacacta
     2101 ctgggattcc ctcttccacc gctttcggct tgcccttgaa aaacagggaa cgattcttcc
     2161 ctccgtttct ctggtcgcac aagagcacga cgacccgttt cggatcctca tcgccacggt
     2221 gatctccctg cggaccaagg atgcggtgac cctgaaagcg agtcgatccc tcttctcccg
     2281 ggcgaagact ccgagcggga tccttgatct caccgaagaa g
//
LOCUS       DAOPUZ010000189         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_49080_length_2317_cov_3.547745, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000189 DAOPUZ010000000
VERSION     DAOPUZ010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49080_length_2317_cov_3.547745"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..838)
                     /gene="rhuM"
                     /locus_tag="PKN79_03875"
                     /pseudo
     CDS             complement(1..838)
                     /gene="rhuM"
                     /locus_tag="PKN79_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            1090..1452
                     /locus_tag="PKN79_03880"
     CDS             1090..1452
                     /locus_tag="PKN79_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HNZ94935.1"
                     /translation="MDPLFQLGIIFAFTFAGDVLSKGLQLPIPGSIIGMVLTLLALLF
                     GVVKERHLDKTGDFLLSHMTFFFIAPAVGLLGYADLVSSIWLRLILVNIISFFLCYLS
                     ASWTVVGVNALIGRLHRG"
     gene            1445..2125
                     /locus_tag="PKN79_03885"
     CDS             1445..2125
                     /locus_tag="PKN79_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HNZ94936.1"
                     /translation="MADPLFGITLSIVAFGIGIRIQKKTGSALANPLIIAMVLVMLVL
                     VVFDIPLEAYEAGGSFITFFLPPVTTVLALSIYRQRKVLQAYLIPIGAGTLTGSIVAV
                     ASTIFLSRAFGLEEIVVRSLASKSVTAAVAVALTDRLGGIPALTIASTVITGMTGNIL
                     APYLGKIFGVTDKVAHGVGIGTSSHALGTSRALELGEIEGSMSSISISFSALWTTLIL
                     AFVFRLAL"
BASE COUNT          441 a          644 c          592 g          640 t
ORIGIN      
        1 tcatcccttt ccgcgaaggc agccgggtat tgaacctgat tgccaacgtg gcggagaccc
       61 tgccggcgtc ctgcaggatc tccctgtccc acaaggtgag aaaaccgttc agtctctgtt
      121 cccaatcttc catggtcatg ggaatatgcc tggcggcttg gacttccgcc atgtcaagat
      181 acgcggagac gatccgctgc atctgtccaa gctcatgatc cgacagataa ttcttggcta
      241 cgacgacatc gtatgtgtgg attttcccgt ttggcacgcc ttcccaggaa gtaagcccca
      301 tatgctcctt ggccgcatcg gctcgctcta caatgacttc ggctgcggtt tgaccgtgta
      361 tcgcataatg cagcttgttc tgaaccgctc aaaaagaatc tctttgtggc ccttgcactc
      421 gggtcataat cgatggcggt ggcatagatg tcggtaatct tctgatagaa tcgtctttcg
      481 gagagccgga tttcacggat tttttcagtt gtttttcaaa gtagtctttt gtgaggatgg
      541 tgccgaaatt cttgagtctc tcgtcatcca tgacccagcc ctgaatcgtg taatccttga
      601 cgatctgatt cgcccacttc ctgaactgaa ccgcccgctc gttgttgacc ttgaatccga
      661 cggcgatgat cgcctgcagg ttatagtggt ttatgtccct cgaaacgttt ctgtcaccct
      721 ctttttgaac tgttcggaat ttcctaacag ttgaaccttc atcaagttca tcgtcattga
      781 atatggtttt tacgtgttcg tttatcgtgg gtatggtcac atcatacagc gttgccatca
      841 tcttttgggt gagccagatg ttttcatctt cgtatcgtat ctccagagca tgttcgccgt
      901 cgccgatcgc tgcgatatac gtcagatatt cagctgcaga tgagcgaatg gagatttcat
      961 ggttcttctt gccatccatt caatgagttc ctccttcgat tccttaaaat accacaaacc
     1021 ggatccgtgc tcaatttcaa gagtccgaac cccttccatc gggaaggcgc tttacagtaa
     1081 ggttgccgta tggatccgct ttttcagctt ggaatcattt tcgccttcac cttcgccggt
     1141 gacgtgttga gcaagggcct tcagcttccg atccccggtt cgatcatcgg gatggtgctt
     1201 acgttgctgg ccctcctctt tggtgtcgtc aaggaacgtc atcttgacaa gacgggggat
     1261 tttcttcttt cccacatgac cttcttcttc atcgccccgg ccgtgggatt actggggtat
     1321 gccgatctgg tctcttcgat ctggctccgg ctcattctgg tgaatatcat ctccttcttc
     1381 ctctgctacc tgagcgcaag ctggaccgtc gtcggggtca atgctctcat cgggaggctg
     1441 caccgtggct gatccgctct ttggcatcac cctctcgatc gtcgccttcg gcatcggtat
     1501 tcggatccag aaaaagacag gaagcgcctt ggccaatccc ctgatcatcg cgatggttct
     1561 ggtcatgctt gtgctcgtcg tctttgacat tccgctggaa gcgtatgagg ccggcggatc
     1621 cttcatcacc ttcttcctcc cgcccgtcac caccgttctg gcgctttcga tctaccgcca
     1681 gcgcaaggtc ctgcaagcgt atctcatccc catcggcgcc gggacgctga caggctcgat
     1741 cgtggcggtc gcctcgacga tcttcttaag ccgcgccttc ggccttgaag agatcgtggt
     1801 gcgctccctt gcaagcaaat ccgtcaccgc tgcggtggcg gtggcgctga ccgaccggct
     1861 tggagggatt cccgccttga cgatcgcctc gaccgtgatc accggaatga cgggaaacat
     1921 ccttgccccc tatctgggga agatcttcgg tgtcaccgac aaggtcgccc acggggtggg
     1981 gatcggaaca tccagccacg cactcggaac gagcagggcg ctcgagctgg gcgagatcga
     2041 gggttcgatg agcagcatct cgatcagctt ttccgccctt tggacgacgt tgattctcgc
     2101 gtttgtgttc cgacttgccc tttgactgtc cctttgttat agtatgggag agttaatggg
     2161 agagtaaggt tcaggtagga ggaaatgatg gcgattccga tcaatatgga agaactgctt
     2221 catcagcgtg tgatagagag cgcccgcata gaatacaaga cggactggaa tcctgcgccg
     2281 attctgcatt ccataaccgc ttttgccaat gattttg
//
LOCUS       DAOPUZ010000190         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_49432_length_2305_cov_3.328889, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000190 DAOPUZ010000000
VERSION     DAOPUZ010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49432_length_2305_cov_3.328889"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PKN79_03890"
     CDS             complement(<1..1202)
                     /locus_tag="PKN79_03890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNZ94937.1"
                     /translation="MATDSIQGRVPPQNEEVERAVLGAILLNDRVLTDVANVLAPGDF
                     YKVAHQQLFAAILAYKQESSEVLDLLTLNAFLKSKALVDQCGGPAYIASLTSDVPSTT
                     NAVYYAQILKALSYRRKLLLYASQLRDRAFDESQDIQKVIDDGEQELSVLSNEAAGSD
                     SYHDIKNLVNTLISDIEERSITGEKRGFDSGFSALDSITGGFKKQEFIIIGARPSIGK
                     TAFALSIVLNMLIKKRIRVGFFSLEMSAFSLMERLLSGHSRVDFTHIRRATLQNREMQ
                     AIVDASAALYDSELYIQDTPNMKLMELRAQARRMKLEHDVQVIFIDYIGLMDAETDSR
                     VPRHEQISIISRSLKQLARELKVPIICLSQVGRQADGIEPKLADLRDSGSIEQDADVV
                     ILLHRDTT"
     gene            complement(1208..1816)
                     /gene="rplI"
                     /locus_tag="PKN79_03895"
     CDS             complement(1208..1816)
                     /gene="rplI"
                     /locus_tag="PKN79_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNZ94938.1"
                     /translation="MKIILNQDVANLGEEGDIVVVKAGYARNFLLPKGMAVLHNKANL
                     AIFASRAEAIEKRKALKRKESASLRDKLSELELKIVVTAGESGRLFGSVTSQMVQNEL
                     SKLGYEIERKKIEVPTHTIKMTGTYTIHVRLYEGDVADVKLVVASESVEKAKAAQPAK
                     EAAEAAKAAEKAAAEAAAAAAAEEVVEEEEAVEEAAAEEDNE"
     gene            complement(1828..>2305)
                     /locus_tag="PKN79_03900"
     CDS             complement(1828..>2305)
                     /locus_tag="PKN79_03900"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HNZ94939.1"
                     /translation="ATMNLLYRTMVMILGAVLAPMMMAIMGFAFYVALSFQSRGSEEP
                     FAVRFSRIRLPENGIWVFLSGWTLVLVFVLVKSGYLPRALAIQCALSISLLYAIQGFA
                     IILFHLVKRNLVHRAGRLGFMVVLLMFLVPGLNLLVVFALPLLGVTETWIAYRRNV"
BASE COUNT          530 a          634 c          601 g          540 t
ORIGIN      
        1 ttcgtggtat cccgatgaag aaggatcacg acgtcggcat cctgctcgat cgagccggag
       61 tcgcgcagat cggccagttt cggctcgatt ccatccgctt ggcgccccac ttgcgaaagg
      121 cagatgatcg ggactttcaa ttcccgggcc aactgtttca acgatcgtga gatgattgag
      181 atctgttcat gacgggggac ccgcgagtcg gtctcagcgt ccatcaggcc gatgtagtcg
      241 atgaagatca cctggacatc gtgttcaagc ttcatccggc gcgcttgggc gcggagttcc
      301 atcagcttca tgttcggcgt gtcctggatg tagagctctg aatcgtagag tgcggcggaa
      361 gcgtcgacga tcgcctgcat ctccctgttt tggagcgtcg cccgtcggat atgcgtgaaa
      421 tcgactcggc tgtgacccga caggaggcgc tccatcagac tgaacgcact catctcaagc
      481 gagaagaacc cgacccggat tctctttttg atcagcatat tcagcacgat cgacagagcg
      541 aaggcggtct ttccgatcga agggcgagcg ccgatgatga tgaactcctg cttcttgaac
      601 cctccggtga tcgagtcgag ggcggaaaat ccgctgtcga agcctcgttt ctctccggtg
      661 attgaacgtt cttcgatatc ggagatcaag gtattaacaa gatttttgat atcgtggtag
      721 gaatcactgc cggctgcctc gttggagagg acgctcagct cctgctcacc gtcatcgatg
      781 actttctgga tatcctgcga ttcatcaaac gcccggtctc gaagctgtga agcatacagg
      841 agaagctttc ttcggtagct gagcgctttg aggatttgag cgtaatacac agcgttggtc
      901 gttgagggaa catcgctggt caagcttgca atataagcag gtccgccaca ttgatcgacg
      961 agcgccttgc ttttgagaaa ggcgttcagc gtgagaaggt ccaagacctc cgagctttcc
     1021 tgcttatacg caagaatggc tgcaaacagc tgttggtgag caactttata aaaatctccc
     1081 ggagcaagga cgttggcgac atccgtcaac acccggtcat tgagaagaat cgctccaagg
     1141 accgctcgtt caacttcttc gttttgagga ggaactcggc cttgtatgct gtctgtagcc
     1201 atatctctta ctcgttatct tcctcggcag ccgcttcttc aacagcttcc tcttcttcga
     1261 caacctcttc cgctgcggca gcggcggctg cttcggcagc tgccttttcc gccgccttgg
     1321 cggcttcagc ggcttccttg gccggctgag cggcttttgc tttttccacg ctttcactgg
     1381 cgacgacaag cttcacatcg gcgacatcgc cttcgtagag acgcacgtgg atcgtgtatg
     1441 taccggtcat tttgatcgta tgggtcggca cctcgatctt tttgcgctca atctcgtatc
     1501 cgagcttgga aagctcattt tggaccatct ggctggtaac ggaaccgaaa agacggccgc
     1561 tttcaccggc ggtaacgacg atcttgagct caagctcact gagcttgtca cgaagcgaag
     1621 cgctttcttt gcgcttaagc gctttccgct tctcgatcgc ttcggcccgg gaggcgaaga
     1681 tcgcaaggtt tgctttattg tgcaacaccg ccatgccttt aggcaggagg aagttgcggg
     1741 catatccggc ttttacaaca acgatatcgc cctcttcacc gaggttcgcg acatcctgat
     1801 taagaataat cttcatagga atactcctta cacatttctt ctatacgcta tccaagtctc
     1861 cgtcactccc agaaggggaa gagcgaagac aaccagcagg ttcaaccctg gcaccaaaaa
     1921 catcaacagt acaaccatga accccaaccg accggctcga tggacaaggt ttcgcttcac
     1981 cagatgaaac aggatgattg caaagccctg aattgcatac agcaagctga tagaaagagc
     2041 acactggatc gcaagggcac gggggaggta gccgctcttg acaagtacaa agacaagcac
     2101 aagcgtccac ccactcaaaa agacccaaat cccgttctcc ggcagacgaa ttcggctgaa
     2161 acggacggca aacggctctt cactgccgcg gctctgaaaa gataatgcga catagaaggc
     2221 aaatcccatg atcgccatca tcatcggagc gagaaccgct ccgaggatca tcaccatcgt
     2281 gcgatagaga agattcatcg tagcg
//
LOCUS       DAOPUZ010000191         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_49519_length_2302_cov_3.926569, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000191 DAOPUZ010000000
VERSION     DAOPUZ010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49519_length_2302_cov_3.926569"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..738)
                     /locus_tag="PKN79_03905"
     CDS             complement(145..738)
                     /locus_tag="PKN79_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNZ94940.1"
                     /translation="MKRCSIIIHSVSGNCFIIGNYLKELMAERNVDARLYRVDDPDLH
                     IWANTQETTNEFYEDICELPLVGDSTLHKSDMIVLGGPTRFGNISAEMKTFLDSTLPL
                     SKDKSLETKFFACFTSCSNSTCEGAHTLNSLIYWAQSMGMLHIPFGVHYELEFSDQPV
                     SGIVHLAGKEDSIRPGDRLGHELEVYADILAAYIQED"
     gene            complement(791..1570)
                     /locus_tag="PKN79_03910"
     CDS             complement(791..1570)
                     /locus_tag="PKN79_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ94941.1"
                     /translation="MRTLIELRHATVQRLGQRILNDVSLTVKRNEHVAIIGPNGAGKS
                     TLVQTLSMEVHPLHTPAFKRILFGKSRWQILELRKHLGIVSASLQHVCTSTYKAWEIV
                     VSGFFSSIGLDFHHLVEESHLKRAKEVMNTYGILPLWEKQMNRLSSGEARRVLLARAA
                     VHEPEVMLLDEAGASLDFPSRHTYRKSLEQLDREGKTIILATHELSEIIPAIGRVVVM
                     KNGAIVADGPKEEIFNEALLREVYEAPIYLDYRDGLYSVWS"
     gene            complement(1589..>2302)
                     /locus_tag="PKN79_03915"
     CDS             complement(1589..>2302)
                     /locus_tag="PKN79_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ94942.1"
                     /translation="QTIERWIKDGTLECGSYLPSQVELAEQFRTSSRPIREALKILEA
                     RGLVVLSQGRRAQIRSNNLDHYVMSVSSTILNSDISHSKLLKNLMQVRLTVASSAARN
                     FSRLEGRKRYLTQLWAASRQMEVSLPGIYDREAQATQSFLDAEMDFHHTLVMANNNQI
                     LSAIYENLSPLLDQAMRSIRFSAGQLDKRAKNYAYLCEALENGQTDLAVALVMVILST
                     LEDKVLDSYPEEQRRASYA"
BASE COUNT          499 a          619 c          671 g          513 t
ORIGIN      
        1 cgtgcgccac tgggacaacc tcaagcatat gagcggaagt catgaggtga gcatccaaag
       61 cgccaccagc gactacaagg cgcgctacgg caaaaccctc gtcggccggt ggcttcataa
      121 gctcaaagag tttcttggaa aggattagtc ctcctgaatg taagcggcga ggatgtcggc
      181 atacacctca agttcatgac cgagtcggtc gccggggcgg atcgaatcct cttttccggc
      241 aagatggacg attcccgaga ccggctggtc gctgaactcc agttcatagt gaaccccgaa
      301 ggggatgtgg agcatgccca tcgattgcgc ccagtagatc agcgagttga gggtgtgcgc
      361 tccctcgcac gtcgagttcg agcaggaggt gaagcaggcg aaaaacttcg tctccaagct
      421 tttatccttg ctgagcggca gcgtggagtc gagaaacgtc ttcatctccg ccgagatgtt
      481 tccgaaccgg gtcggtccgc cgaggacgat catatcgctt ttgtgcagag tggaatctcc
      541 gaccagcgga agctcgcaga tgtcttcgta gaactcattg gtcgtctcct gggtgttcgc
      601 ccagatgtgg agatcggggt cgtcgactcg atagaggcgg gcatcgacgt tccgttccgc
      661 catcaattct ttcagatagt ttccgatgat aaaacagttg ccactgacgc tatggatgat
      721 aatcgaacaa cgtttcatga ggtaccctcg ctttgtatcg tataccccaa gtatacacca
      781 acccgagcgt ttaagaccac accgaataca gcccgtcccg gtaatcaagg tagatcggcg
      841 cctcatacac ttcacggaga agcgcttcat tgaaaatctc ctctttcgga ccgtcagcca
      901 cgatcgcgcc gtttttcatc accaccaccc ggccgatagc cgggatgatc tcactgagct
      961 catgggtggc gagaataatc gttttccctt cccggtcgag ctgctcgagg cttttgcgat
     1021 aggtgtggcg gctggggaaa tccaacgagg cgcccgcctc atcaaggagc atcacctccg
     1081 gctcatgcac cgccgcccgg gccaacagaa cccgacgcgc ttcgccgctg gagaggcggt
     1141 tcatctgttt ctcccacagc gggagaatcc cgtaggtgtt catgacctcc tttgcccgct
     1201 taaggtggga ttcttccacg agatggtgga aatccaagcc gatcgaactg aagaaaccgc
     1261 tgacgacgat ctcccaggcc ttgtaggtgg atgtacagac atgttgaagg ctcgccgata
     1321 cgatgccgag atgctttcgc agctccagaa tctgccatcg ggactttcca aagagaatcc
     1381 gtttgaaagc gggggtgtgg aggggatgga cttccatact gagcgtctga accagcgtcg
     1441 actttcccgc tccgttggga ccgatgatcg cgacgtgttc gttccgcttg acggtcaggg
     1501 agacatcgtt aagaatccgc tggccaaggc gctggacggt agcgtgtctg agttcaatga
     1561 gggtgcgcat acggcgagta tagcacggct aggcgtacga ggcgcggcgt tgttcctcag
     1621 gataggaatc gagaactttg tcctccagcg tagagaggat gaccatcacc aaggcgaccg
     1681 cgaggtcggt ctggccgttt tccagcgctt cgcagaggta ggcgtagttt ttcgcccgtt
     1741 tgtccaattg accggcgctg aagcggatgg aacgcatcgc ctgatcgaga aggggagaga
     1801 ggttctcata gatggcgctt aagatctggt tgttgttcgc catcaccagg gtatggtgga
     1861 aatccatctc ggcatcaagg aaggattggg tcgcctgagc ttcccggtca tagattccgg
     1921 ggagggagac ctccatctgt cggctcgccg cccacagctg ggtaagatat cgctttcttc
     1981 cttcgagacg ggaaaagttc cgagctgcgc tgctggcgac ggtaaggcgc acctgcatca
     2041 ggttcttcaa cagtttggag tggctgatat cgctgttcag aatcgtggaa gagacactca
     2101 ttacatagtg atcaaggttg ttggagcgga tctgagcccg ccggccctgg gaaagaacca
     2161 ccagtccccg ggcttcgaga atcttcaacg cttcccggat tggacggctg gatgtcctaa
     2221 actgttctgc aagttcgact tgagagggta ggtaggagcc gcactccaat gtgccgtctt
     2281 taatccatcg ttcaatcgtc tg
//
LOCUS       DAOPUZ010000192         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_49603_length_2300_cov_2.659688, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000192 DAOPUZ010000000
VERSION     DAOPUZ010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49603_length_2300_cov_2.659688"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PKN79_03920"
     CDS             <1..632
                     /locus_tag="PKN79_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNZ94943.1"
                     /translation="VSDRFIPILEQTGLIYDVGLWVLEEALLEHTRLIEKGFPPLRIS
                     INLSVVQLQAEHFFEDFLAVIRKSGVAPGYIELEITESFFLENPLDTLEKIYKLKELG
                     VSIAIDDFGKGHSSFNRLKTVPFDRLKIDKEIIDTIDVDKRLAPLTEIIILLARTLGA
                     SVTAEGVERKEQADFLKTVDCDEIQGFYYSRSLSPEALEEFLRNETRSS"
     gene            complement(566..>2300)
                     /locus_tag="PKN79_03925"
     CDS             complement(566..>2300)
                     /locus_tag="PKN79_03925"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HNZ94944.1"
                     /translation="DGEDPEVLITIDVVNEAKGRHIRPLDGMIPADALLWSTYREDRL
                     LMQRFNELTDKVLDSKRGYYGFVGRKTVIKSCDTIKDVWIGEGAYIKGANKLKNLTLR
                     STLAEPVQIGEGVELVNGIIGAGSRIFYGSKAIRFVMGDNSNLKYGARLIHSVLGDNS
                     TVSCCEILNSLIFGSHEQHHNNSFLVAARIEGQSNMAAGANLGSNHNSRNNDGEMIAR
                     RGFWPALSTTVTYDSYFASYTLIAKGSFPHQLSVPLPFSLISSDEISNRRVIMGAYWW
                     MYNLYALERNTHKYHSRDKRKAPRQRYEPAYLAPDTANEIIEARGLLELWVGESLGDG
                     SIDKDSLRAQGKFLLANQAELVGRSTITTYTVENSKEPVIVLKAVESYRAYGEMLLFY
                     AMKTLVAYAANRDQVIDDLLGKRESHLSPWENVGGQLVRRDRVEALLQDLKEGSIKSW
                     DEVHQRYSEWEDSYEEDTLSHALGVLSFLLGEKRLTDDLWKSLVDETKVLVQKIEQGI
                     YHSKEKDHLNHFKYTTYRNEEERDAVLGNLKDDLLIKTVHKEMETFLDQLERVSFLRN
                     SSNASGDRDLE"
BASE COUNT          484 a          634 c          652 g          530 t
ORIGIN      
        1 tggtatcgga tcggttcatc cccatcctcg aacagacggg cctgatctat gatgtgggac
       61 tctgggtgct ggaagaagcc cttctggagc atacccggct gatcgaaaaa gggtttccgc
      121 cgcttcgtat ttcgataaac ctgtccgtcg tccagcttca ggcggaacat ttcttcgagg
      181 atttccttgc ggtcatcaga aaaagcgggg tggcgcccgg ctacatcgag ctggagataa
      241 cggaaagctt cttcctggaa aatccgctgg acacccttga gaagatatac aagctgaaag
      301 aactgggtgt cagcatcgcc atcgacgatt tcggcaaggg acattcatcg ttcaaccggt
      361 tgaaaaccgt cccgttcgac cggttgaaga tcgacaagga gatcatcgat acgatcgatg
      421 tggacaaacg tctggctccc ctgacggaaa tcatcatcct gctggcccga accttggggg
      481 caagcgttac ggccgaaggc gtggaacgga aggagcaggc ggatttcctg aaaaccgtcg
      541 actgcgacga gatccagggg ttctattact caagatccct gtcgccggaa gcgttggagg
      601 agttcctcag aaacgagacg cgttccagct gatcgaggaa ggtctccatc tccttatgga
      661 cggtcttaat cagcagatca tccttcagat tccccaagac cgcgtcgcgc tcttcttcat
      721 tacggtaggt ggtgtacttg aagtgattga gatgatcctt ctccttggag tggtagatcc
      781 cctgctcaat cttttgaaca agaactttgg tttcatcaac gagactcttc cacagatcat
      841 cggtcagtct cttctcccca aggaggaagg agagcacccc gagggcatgg gacaaggtgt
      901 cctcttcata ggagtcctcc cactcggaat accgctgatg aacctcatcc cacgacttga
      961 tcgatccttc tttgaggtct tgaaggagcg cttccacccg gtcgcgccga acaagctgtc
     1021 caccgacgtt ttcccaagga ctcaggtgag attctctctt cccaagaaga tcgtcaatca
     1081 cctgatcacg gttggcggca taggcaacca aggtcttcat cgcgtagaaa aggagcatct
     1141 cgccataggc ccggtaggat tcaacggctt tgaggacgat caccggttcc ttcgagttct
     1201 ccaccgtata agtggtgatc gttgaacgcc ccaccaactc ggcttgattg gcaagcaaga
     1261 acttgccttg agctcgaagg gaatccttat cgatgcttcc gtctcccaag ctctcgccta
     1321 cccagagttc aagcaagcct cgcgcctcga tgatctcatt cgccgtatcg ggggcaaggt
     1381 aggcgggctc atagcgctgt cttggagcct tgcgcttgtc gcggctgtgg tatttatggg
     1441 tgttccgctc caatgcatag aggttgtaca tccaccagta cgctcccatg atcacccggc
     1501 ggttgctgat ctcgtcactg gaaatcaggc tgaacggcag gggaacggaa agctggtggg
     1561 gaaaggagcc tttggcgatc agggtatagc tggcgaaata tgaatcatag gtaacggtgg
     1621 tggagagcgc cggccagaac cccctgcggg cgatcatctc cccgtcgttg ttccggctgt
     1681 tgtggttcga cccgaggttc gccccggcgg ccatgttgga ttgtccttcg atccgggcgg
     1741 cgacgaggaa ggagttgttg tggtgctgtt cgtgcgaacc gaagatcagg ctgttgagga
     1801 tctcgcagca gctgaccgtg gagttgtccc ccaacacgga gtggatcaag cgggcgccgt
     1861 atttgaggtt ggagttgtcc cccattacga agcggatcgc tttggagccg tagaagatcc
     1921 gggagcccgc gccgatgatc ccgttgacca gttcgactcc ctcgccgatc tgaaccggct
     1981 cggccagagt cgatcggagg gtcaggttct tcagcttgtt cgctcctttg atatacgccc
     2041 cttcgccgat ccatacatcc ttgatcgtgt cgcacgattt gatcacggtc ttcctgccga
     2101 cgaagccgta gtagccccgc ttggaatcga ggaccttgtc ggtaagctcg ttgaaccgct
     2161 gcatgagcag ccggtcttcc cggtaggtcg accagaggag cgcatcggcg ggaatcatcc
     2221 cgtccaaggg ccgaatatgc cgtcccttcg cctcgttgac gacatcgatg gtgatgagga
     2281 cctcggggtc ttctccgtcc
//
LOCUS       DAOPUZ010000193         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_49623_length_2299_cov_2.835116, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000193 DAOPUZ010000000
VERSION     DAOPUZ010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49623_length_2299_cov_2.835116"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PKN79_03930"
     CDS             <1..544
                     /locus_tag="PKN79_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNZ94945.1"
                     /translation="HNVEVTSDIAKVLLSGIVSDTIMLKSPTTTFEDYTAVQDLLSIA
                     GVDDMYKFGETMFSGGASLAKSDARMMIEADFKRYRESGVNFGIGQSEVTTLDDVEDY
                     RARYLEELEMVKKAYSLDWALFLITDVVKENSVLLLTRMPIAEQKLAYEKAGEGMYLL
                     PQVLSRKKQLLPEIIRVIQE"
     gene            complement(546..>2299)
                     /locus_tag="PKN79_03935"
     CDS             complement(546..>2299)
                     /locus_tag="PKN79_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNZ94946.1"
                     /translation="DMVEGETKIASIKTMSKSVQEAETIRKMFFAMSEDIRVIIIKLA
                     DKLHNMRTLHHLKNERAREIANDTLDIYAPLADRLGISWMKDELEDLSLKTLKPDTYN
                     HIQEYLLSKKSEQTAYLTRVEKSIYRACGDANIADVVVTSRAKHAYSVYMKMKKRKKE
                     IDEIFDILGVRILCTSVIDCYTILGVVHRLWPPIEGRFKDYIAMPKANNYQSLHTTVM
                     ALDGKLLEIQIRTHEMHFTAEYGVAAHWSYKAETGSESGNWAQMDDEQFTKIVSKLKA
                     WSSEIENSESYMDDIKGDLLRDTIYVFTPKGNIVELPKNATALDFAYQIHTEVGNRTT
                     GAKADGSIIPLNAPLKNTQVIEILTSPNARPHLNWLRYAQTNSARKKIRAWLNKYDEN
                     ILIDKQIIAKRKEIEQAQAKIEKRPPEQQVEGDDDEIIRQHVDPSRMKFRVGDEKNMM
                     IHIAQCCNPVRGDEIIGYVSRGRGIIVHRTDCPNLKNMAEVKDRSIDVEWETEFPKLT
                     KRFAVTSKRTYDLFGEIEGALRKYKGHLIEGRLQDDEDGKLMGTFTMEVQQEDDFKKI
                     IKTLKTIPSINTISEIR"
BASE COUNT          426 a          610 c          641 g          622 t
ORIGIN      
        1 ccacaacgtt gaagtgacga gtgatatcgc caaagtcctc ctttcgggaa tcgtcagcga
       61 tacgatcatg ctcaaaagtc ccaccacgac attcgaagac tacaccgcag tgcaggatct
      121 tctctcgatc gccggagtgg atgacatgta caaattcggg gaaacgatgt tctccggcgg
      181 ggcttctctg gctaaaagtg atgccagaat gatgattgag gctgacttta agcgctatcg
      241 ggaaagcgga gtcaacttcg gcatcggtca aagcgaggta accacccttg atgacgtcga
      301 ggattatcgc gctcggtatc ttgaggagct ggagatggtg aaaaaggcct acagcctcga
      361 ctgggcgctc ttcctgatca ccgacgtcgt caaggagaac tccgtgctgc tccttacccg
      421 gatgccgatc gctgagcaga agctcgccta cgaaaaggcc ggtgaaggga tgtacttgtt
      481 gccacaagtc ctctcccgta aaaagcagct gttgcccgag atcattcggg tgattcagga
      541 gtaatctagc gaatctcgct gattgtgttg atcgaaggaa tcgttttcag ggtcttgatg
      601 atcttcttga agtcatcctc ctgctgaacc tccatcgtga aggttcccat cagcttgccg
      661 tcctcatcgt cctgaaggcg gccttcgatc aggtggccct tatacttcct gagcgcgcct
      721 tcgatctcgc cgaagaggtc gtaggtgcgt ttgctcgtca cggcgaaccg cttggtcagc
      781 ttggggaatt ccgtttccca ttcgacatcg atcgaccggt ccttcacctc ggccatgttt
      841 ttcaggttgg ggcaatcggt gcggtgaacg atgattcccc ggccccggct gacgtagccg
      901 atgatctcat cgcctctgac cgggttgcag cactgggcga tgtggatcat catgttcttc
      961 tcgtccccga cccggaattt catccgtgaa ggatcgacgt gttggcggat gatctcatca
     1021 tcgtcccctt cgacctgttg ttcagggggc cgtttttcga tcttcgcctg ggcctgttcg
     1081 atctctttcc gcttggcgat gatctgtttg tcgatgagga tattctcatc gtacttgttc
     1141 agccacgccc gaattttctt ccgggcgctg ttcgtctggg cgtagcgcag ccagttcaaa
     1201 tgcggtcggg cgttcgggct ggtgaggatc tcgatgacct gggtgttttt cagcggagcg
     1261 ttcaggggga tgatcgatcc gtccgctttc gctccggtgg tccggtttcc gacctcggtg
     1321 tggatctgat aggcgaaatc caacgcagtg gcgttcttcg gcagctcgac gatgttccct
     1381 ttgggggtga agacgtagat cgtatccctt aagagatcac ccttgatgtc atccatgtag
     1441 gattcgctgt tttcaatttc gctggaccat gctttgagct tgctgacgat cttggtgaac
     1501 tgctcatcat ccatctgcgc ccaattaccg ctttcactgc ccgtttccgc tttgtaggac
     1561 cagtgggcgg cgactccgta ttcggcggtg aaatgcattt cgtgggtcct gatttggatt
     1621 tcaagaagtt tgccgtcgag cgccatcacg gtggtgtgaa ggctttggta gttgttcgcc
     1681 ttcggcatgg cgatatagtc cttgaaccgc ccttcgatcg ggggccagag gcggtggacg
     1741 acccccagga tcgtgtagca gtcgattacc gaggtgcaga gaatccgcac tccgaggatg
     1801 tcgaagatct catcaatctc ctttttccgc ttcttcatct tcatgtagac agagtaagca
     1861 tgtttggcgc ggctggtgac cactacatcg gcgatgttcg catccccgca cgcccgatag
     1921 atcgatttct ccactcgagt caggtaggcg gtctgctcgc ttttctttga cagaagatat
     1981 tcctgaatgt ggttgtaggt gtcgggcttg agggttttca gactgaggtc ctcaagttcg
     2041 tccttcatcc aggagatgcc gaggcggtcc gccaagggcg cgtagatgtc cagcgtatca
     2101 ttggcgattt cccgtgcccg ctcattcttc agatggtgaa gcgtccgcat gttgtgcaac
     2161 ttgtcggcaa gcttgatgat gatgacgcga atgtcttcgc tcatcgcgaa gaacattttt
     2221 cggatcgttt ccgcctcttg gaccgatttg ctcatcgtct tgatgcttgc gatcttcgtt
     2281 tcgccctcca ccatgtcgg
//
LOCUS       DAOPUZ010000194         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_49820_length_2293_cov_2.902145, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000194 DAOPUZ010000000
VERSION     DAOPUZ010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49820_length_2293_cov_2.902145"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PKN79_03940"
     CDS             <1..970
                     /locus_tag="PKN79_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ94947.1"
                     /translation="IADLDDMWTVEGKRTLKSTFDRWEGGLSRTFELRFITESEYFTR
                     DVDPRRPTSAEIESASKNALLSSPFTLDNFRKVVQARHFSRSLIFSFVYPFLGALISI
                     LMGIMAALLLNRTFRGQSFLRGLFLFPYISPIIAATFVWVFFLDPFSGTVNALLLDTG
                     VIDAPIPFLNAWTIDISLFGMKVPFPLARAMVIFFDAWRYFPFAFLFILSRLQAIPED
                     LYEAADVDGAGYFKKFRHITLPQISSVLGTIFLLRFIWTFNKFDDVFLLTGGSAGTKT
                     LPVEVYDNAFGRGDIGAGAATAVLLFLMLSIFMALYFKLLKEVDDE"
     gene            963..1235
                     /locus_tag="PKN79_03945"
     CDS             963..1235
                     /locus_tag="PKN79_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94948.1"
                     /translation="MNDRAISGLGMTVALVLGVFYTILSEFIWFLAVSLLGKTNAFPP
                     LLPMVVAGVLVGLSNGYCKVRIRGEKRQILFSVGAGLIIALIWLLL"
     gene            1278..2198
                     /locus_tag="PKN79_03950"
     CDS             1278..2198
                     /locus_tag="PKN79_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ94949.1"
                     /translation="MITFPIIKLLSRNAMKDIPLSRTTKGHVELMVVKILRAFGLFFF
                     TLIVVFPFYFMLATSLKSRAEYLMDPANLSVNLLQYPSKLFGGYIEVLFRFGFVRFIM
                     NSAIVAALTVVITLIPSILGAYAVTRLRFRGRDLLSRLILLIYMFPAIVLVIPLYSVF
                     TQAGLKDTRLGLLIIYAAMTIPVALYMLRSYFLTLPADLEEAGLIDGLSRLGVIIRIT
                     IPLAMPAIAAIGLYVFMIAWNEFLFAYMFLDTPEIFTLARGMVMLDDQEVPRQLLMAG
                     AVIITVPIMVLFIRFQKHLVGGLTAGGVKG"
BASE COUNT          428 a          643 c          580 g          642 t
ORIGIN      
        1 catagccgat cttgatgata tgtggacggt ggagggcaag cgaacgctga agagcacctt
       61 cgatcgttgg gaaggcggct tgtcccgaac ctttgagctt cgcttcatca cggaatccga
      121 gtatttcacc cgtgatgtcg atccgcgccg ccccacatcg gccgagatcg aatcggcatc
      181 aaaaaacgcc cttttgtcaa gtcccttcac cttggacaac tttcggaaag tcgtccaggc
      241 gagacatttc tcccgttctc tcatcttcag tttcgtctac ccctttctgg gagcgctgat
      301 atcgatcctg atgggaatca tggcggctct tttgctgaac aggacattcc ggggtcagag
      361 ctttttgcgg ggactgttcc tgttccccta catctccccc atcatcgcgg ctaccttcgt
      421 ctgggtcttt ttcctcgatc cgttcagcgg tacggtgaac gcacttctcc tcgataccgg
      481 agtgatcgac gcccccatac cctttttgaa cgcatggacg atcgacatct ccctcttcgg
      541 gatgaaggtt cccttccctc tagctcgggc gatggtgatt ttcttcgacg cttggcgcta
      601 tttccccttc gcgttcctct tcattctctc caggcttcag gcgatccctg aagatctgta
      661 tgaagccgcc gatgtggatg gagccggcta cttcaagaaa ttccggcata tcaccctgcc
      721 gcagatcagc tcagtgctgg gaacgatctt tttgctgaga ttcatctgga ccttcaacaa
      781 gttcgatgat gtgttcctgc ttaccggagg atcggccggc acgaagaccc tcccggtcga
      841 agtctatgac aacgccttcg gtcggggaga tatcggagcc ggggcggcga ctgcggttct
      901 tctgttcctg atgctcagca tcttcatggc tctctacttt aaacttttga aggaggttga
      961 tgatgaatga tagagcgata agcggcttgg gcatgaccgt cgcactggtg ctgggtgtct
     1021 tttatacgat cctttcagag ttcatctggt ttctcgccgt ctccctgctt ggcaaaacca
     1081 acgccttccc tcctcttctt ccgatggtcg tggctggagt gctcgtaggg ctttcaaatg
     1141 gttattgcaa ggttcggata cggggtgaaa agcggcagat cctcttttcg gttggagccg
     1201 gattgatcat cgccctgatt tggctcctgc tctagggttt caaggcgtcc ctcaccgtaa
     1261 aagatgtttt cggagcgttg atcaccttcc cgatcatcaa gcttctttca agaaacgcga
     1321 tgaaggatat tcccctttca aggactacga agggacacgt cgaactgatg gtcgtgaaga
     1381 tcctcagggc gttcggactc tttttcttca ccctgatcgt cgtctttccc ttctatttca
     1441 tgctcgccac atcattgaag tcgagagcgg aatatctgat ggatccggcc aacctctcgg
     1501 tcaaccttct tcagtatcct tcgaaactgt tcggcgggta tatcgaagtg ctcttcaggt
     1561 tcggtttcgt gcgattcatt atgaacagcg cgatcgtcgc cgcccttacg gtggtcatca
     1621 cgctgattcc ttcgattctg ggggcctatg cggtaacgag gctccgcttt cgtggcaggg
     1681 atcttctttc cagacttatc ttgctcatct acatgtttcc cgcaatcgtt ctggtcatcc
     1741 ccttgtacag cgtcttcact caagcagggt tgaaagacac tcggctcggg ttgctcatta
     1801 tctatgccgc catgacgatt ccggtcgccc tctacatgct gcgtagctac ttccttacgc
     1861 tgccggcgga tcttgaggaa gcgggcctga tcgacggact ctcccgattg ggcgtcatca
     1921 tcagaatcac gatccccttg gcaatgccgg cgatcgctgc gatcgggctc tacgtcttca
     1981 tgatcgcatg gaacgagttc ctcttcgcct acatgttcct cgacaccccg gagatcttta
     2041 ccctcgcccg agggatggtc atgcttgacg accaggaggt tcccagacag ttgctgatgg
     2101 cgggagccgt gatcatcacc gttccgatca tggttctctt tattcggttc cagaaacatc
     2161 ttgtgggagg attgaccgcc ggaggcgtca agggataaag agagcctctc cttttatagt
     2221 tagaacctgg acaattcgct gtccgggttc ttcttttctt ctcggatgat ctgctatgcc
     2281 aaattcctta ggg
//
LOCUS       DAOPUZ010000195         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_50020_length_2287_cov_2.889785, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000195 DAOPUZ010000000
VERSION     DAOPUZ010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50020_length_2287_cov_2.889785"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..306
                     /locus_tag="PKN79_03955"
     CDS             73..306
                     /locus_tag="PKN79_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNZ94950.1"
                     /translation="MEKDLVEYIVKSLVDLPDEVDVNVIEGEKSTILELRVASEDVGK
                     VIGKQGRIAKAIRTILSASATRDGKRAVLEILD"
     gene            308..814
                     /gene="rimM"
                     /locus_tag="PKN79_03960"
     CDS             308..814
                     /gene="rimM"
                     /locus_tag="PKN79_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNZ94951.1"
                     /translation="MDRLIATATIGRTFGYEGEVRVYPNNEESDYLTKLKTIVASLKD
                     GKETTLAVEYVRADGKSVLMKFKGYDSSEEAQTLVGARVMVERKFASPLKEGEYYTAD
                     LIGCTLVSGGEALAMVVSVLDGPQALLLEAERPDSKRFLVPFLYEYTGEVDLEAKTIE
                     LLSPWLLD"
     gene            811..1572
                     /gene="trmD"
                     /locus_tag="PKN79_03965"
     CDS             811..1572
                     /gene="trmD"
                     /locus_tag="PKN79_03965"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNZ94952.1"
                     /translation="MTITILTLFPHMLEGFFTSSIMRRAVEKGTIAYKLIDFRSWATD
                     KHKSVDDLPYGGGAGMVLMYDPLSKALDELGTRGKRVVYPSPSGVLLNQKLAHELSEE
                     RELIFICGHYEGVDQRIIDEYVDDEISIGDYVITSGEVATMVIVDSVFRLIDGVISRK
                     SLDEESFSDGLLEYPQYTRSEPSCPKEVPHVLLSGHHEKIESWRLKQKLLRTMERRID
                     LLETASLDVVSRKQVNELKEHMAKGIVDNGRDESC"
     gene            1550..1906
                     /gene="rplS"
                     /locus_tag="PKN79_03970"
     CDS             1550..1906
                     /gene="rplS"
                     /locus_tag="PKN79_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNZ94953.1"
                     /translation="MDVMKAVESVQLKENAENFSVGDTVKVHFKIVEGATERIQVFEG
                     LVIAKNNSGVRRTFTVRKISYGVGVERIFPLHSPRVERVEVVRLGRVRRAKLYYIRQR
                     VGKKAKVKELIRKKNA"
     gene            2107..>2287
                     /locus_tag="PKN79_03975"
     CDS             2107..>2287
                     /locus_tag="PKN79_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ94954.1"
                     /translation="MMHALNILIIGLVFLAVVVALFAYRNLNYDYTNETFMKKTGKKL
                     GFTEKQVVLDDGSVLN"
BASE COUNT          572 a          525 c          682 g          508 t
ORIGIN      
        1 ttcgcaattt gctcaacaag agcggcgtgc aggtgaatcg caaggctcag gactaagtcc
       61 taggagcaca ctgtggaaaa agatctagtt gaatatatcg taaagtcgct tgtcgatctt
      121 cccgatgaag tggatgtcaa tgtgatcgag ggcgaaaagt ccacgattct ggaactccgc
      181 gtcgccagcg aagatgtcgg caaggtcatc ggcaagcagg ggcggatcgc caaagcgatc
      241 agaacgattc tcagcgcctc cgccacccgg gatggaaagc gggcagtgct ggagatcctc
      301 gactgacgtg gatcgactca tcgctaccgc tacgatcggg cgaaccttcg gctacgaggg
      361 tgaggtcaga gtctatccca acaatgagga gtccgattac cttacaaagc tcaagacgat
      421 cgtcgcttcc ctgaaagacg gcaaagagac aacacttgcc gtcgaatatg tgcgagcgga
      481 cggcaaaagc gttttgatga aattcaaagg gtatgattct tcggaagagg cacagaccct
      541 tgtcggtgcc cgggtgatgg tcgagaggaa gtttgcttcc ccgctcaagg aaggcgagta
      601 ctacactgcg gatctgatcg ggtgcactct tgtgtccggg ggagaagctc ttgccatggt
      661 tgtcagtgtg ctggatgggc ctcaggcact gcttctggag gccgaacggc cggactccaa
      721 gcgcttcctg gttccgtttc tctacgagta taccggagaa gtcgatctcg aagcgaaaac
      781 gattgagttg ttatccccat ggctcctcga ctgacgatta cgattctaac cctctttccc
      841 cacatgctcg aggggttttt tacctcatcg atcatgaggc gggcggttga aaaggggacg
      901 atagcatata agctcatcga ctttcgttct tgggcaactg acaagcacaa gagcgtcgac
      961 gaccttccct acggaggcgg ggcggggatg gtgttgatgt acgatccgct ttccaaagca
     1021 ttggatgagc tggggacaag ggggaagcga gtggtgtatc cctcaccgtc gggggtgctg
     1081 ctcaaccaga aactggccca tgaactcagt gaagagcggg aacttatctt catctgcggg
     1141 cactatgaag gggtcgatca gcgaatcatc gacgagtatg tggatgatga gatctcgatc
     1201 ggcgattatg tgatcaccag cggcgaagtc gccacgatgg tgatcgtgga ttcggtcttc
     1261 cggctgatcg acggcgtgat cagccgcaaa tcgcttgatg aggaaagctt cagtgacggg
     1321 ttgttggaat atccccagta cacgcgaagc gaaccctctt gcccaaaaga agtccctcat
     1381 gtattattga gcggtcatca tgagaagatc gaatcatggc gactgaaaca gaagctgctg
     1441 cggacgatgg agcggcggat tgatctgttg gaaacagctt ctttggatgt ggtttccaga
     1501 aaacaagtaa atgaattgaa agaacatatg gcgaagggga tcgtagacaa tggacgtgat
     1561 gaaagctgtt gaatcagtac aacttaagga gaatgcagag aacttctccg tcggagatac
     1621 cgtaaaagta cattttaaaa tcgtcgaagg ggcaaccgag cggatccagg tgtttgaagg
     1681 cttggtcatc gcgaagaaca atagcggagt tcgccgaacc tttaccgtgc gaaagatttc
     1741 ctatggggtc ggggtcgagc ggatcttccc gttgcactcg cctcgagtcg aacgtgtcga
     1801 ggtcgtccgt ctcggtcgag tccggagagc gaaactctac tatatccggc agcgggtcgg
     1861 taagaaagca aaggttaagg agcttatccg caagaagaac gcctaagaac aggaatgctg
     1921 ataccgacag aagcggcgtg gatgatcgtc catgccgttt ttctttgatg ggggagtgga
     1981 agagagcgga tctatcccct tgccgcatca ccggggtaga cgatggcttt gcggcgtgga
     2041 atggtgattg acaggtgaac gtgtctggga cggcttccgc ctggatcaca catcacggag
     2101 ggcaagatga tgcacgcgtt gaacatactg atcatcggtt tggtctttct ggctgtcgtg
     2161 gtcgccctgt ttgcctatag gaatctcaat tacgattata cgaacgaaac cttcatgaag
     2221 aagacgggca aaaagcttgg ctttacggaa aaacaggtag ttctggatga cggctccgta
     2281 ctgaact
//
LOCUS       DAOPUZ010000196         2285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_50087_length_2285_cov_2.677578, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000196 DAOPUZ010000000
VERSION     DAOPUZ010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50087_length_2285_cov_2.677578"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="PKN79_03980"
     CDS             <1..697
                     /locus_tag="PKN79_03980"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HNZ94955.1"
                     /translation="THSAVGERSFAYTPKEQIDPSWFAKAREALKEPAFETKRTDEEA
                     DIWTLQQSDESFTYLGQAFNLFLIAQKGDDLFLVDQHAAHERILYDQVRAKQPIQNLM
                     IPIVFEVERSIDEYLMSNLDSYLNLGIKLRKVEDLAWELLGVPALYRTIEHEVIAFLQ
                     TMQGEPSEIEKGLYSVVACHAAIKAGDRIDSTAATELLKSVFALEEPICPHGRTFVIR
                     LNKDDLMTSVGRT"
     gene            complement(694..1314)
                     /locus_tag="PKN79_03985"
     CDS             complement(694..1314)
                     /locus_tag="PKN79_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94956.1"
                     /translation="MQTRSDHSYLSKLVSHKLFCLFLIFFVVFSVGGCKGEEFDVLNV
                     ESSLSALYFLPEGASASILTVSFEVTSDDSVLEVQVSTPDKTSRWVVSVKRDKQGRFT
                     VGPLSMGKGNTLTEGMYSMVILNEQGKTIDYSFPVRSPNTTVDPLQWGSFDSDARTLV
                     MNRSATLQVGNDHVDLAEGESYIFGPDDHEFYLAFDQGATIVRVTL"
     gene            complement(1265..>2285)
                     /locus_tag="PKN79_03990"
     CDS             complement(1265..>2285)
                     /locus_tag="PKN79_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNZ94957.1"
                     /translation="DPPRTNDERLALQESVKHIVSLLDHVTTYEEFQRTITAGFFLPK
                     IVEKSVTAFTHSGTEKLANDQAYLYVSNHRDIILDCALIDLALAQADQMLMEMAIGDN
                     LLTNQFVTDLFKLNGGIVVKRTLPLREKYLESLRLSAYFVESISERNQSIWVAQKSGR
                     SKDGIDETNPAIIKMLHLSQKRKGVSFSEVIKLSRIVPVAVSYEYDPLDVNKAAEQLR
                     KQSDKRPYEDVISMNKGLKGFKGRIHIAFGTPLTEAEYHTPEEVANEINRQIHLNYKL
                     WPTNYFAYDHVNNTTTFAEQYRDFNHETFLRRFAFRREEVRDFALNAYANPVRSFLSQ
                     QARLS"
BASE COUNT          552 a          594 c          588 g          551 t
ORIGIN      
        1 aacccattca gcggtcggtg aacgctcatt cgcctatacg ccgaaggaac aaatcgatcc
       61 ttcctggttc gccaaagcac gggaagcatt gaaagagccc gcctttgaga cgaagcgaac
      121 cgatgaagag gcagatatct ggaccctcca gcaaagtgat gagagcttca cctacctcgg
      181 tcaggcgttc aacctctttt taatcgcaca aaagggcgac gatctttttc tggtcgatca
      241 acacgccgcc catgagcgaa tcctgtatga tcaggtacga gcaaaacagc caattcaaaa
      301 cctgatgatt cccatcgtct tcgaggtgga gcggtcgatc gacgagtacc tgatgtcaaa
      361 cctcgacagc tatctcaacc tgggaatcaa gctcaggaag gtcgaagacc ttgcctggga
      421 gctgctcggt gttcccgctc tttatcggac gatcgagcat gaagtcatcg cctttctgca
      481 gacgatgcag ggggaaccga gcgagattga aaaagggctt tattcggtcg ttgcgtgcca
      541 tgctgcgatt aaagcgggag atcgcatcga ctccaccgcc gcgacggagc tcctgaaaag
      601 cgtctttgcc cttgaagagc cgatctgccc ccatggccga accttcgtca ttcgcttgaa
      661 taaggatgat ttgatgacct cggtcggtcg aacctaaaga gtgacccgaa cgatcgtcgc
      721 cccctgatcg aaggcaaggt aaaactcatg atcgtcgggg ccgaagatat aactctcccc
      781 ttccgcaaga tcaacatgat cattccccac ttggagcgtt gcactccggt tcatcaccag
      841 tgtccgggca tcactgtcaa atgaccccca ttggagggga tcgacggtgg tgttcggtga
      901 gcggaccgga aaggaataat cgatcgtctt tccctgttca ttgagaatca ccattgaata
      961 catcccttcg gtgagggtgt ttcccttccc catcgatagc ggtccgacgg tgaaccgtcc
     1021 ctgtttatcg cgctttaccg agacgaccca gcgggaagtc ttatcggggg tggagacttg
     1081 cacctccaac acggaatcat cgcttgttac ttcaaagctc actgtgagga tcgaggcact
     1141 cgctccttca gggagaaagt acagcgccga gagagacgat tcgacattca gaacgtcgaa
     1201 ctcctcccct ttgcacccgc caacactgaa aacaacgaag aagatcagaa aaagacagaa
     1261 cagtttatga gagacgagct tgctgagata agaatgatct gaccgggttt gcatatgcat
     1321 taagggcaaa gtctcgaacc tcctctcgac ggaatgcaaa gcgacgcaag aatgtctcat
     1381 gattgaaatc acgatactgt tcggcgaatg tcgtggtgtt gtttacatga tcatacgcga
     1441 aataattggt tggccaaagc ttgtagttga gatgaatctg tcgattgatt tcattggcca
     1501 cttcctcggg tgtgtgatac tcagcctcgg tcaacggtgt gccgaaggcg atatgaattc
     1561 gacccttgaa ccctttcaag cctttattca tcgaaatcac atcctcatac ggacgtttat
     1621 cgctttgttt gcggagttgt tccgctgctt tattcacatc cagaggatcg tactcatagc
     1681 tgaccgctac cggaacgatg cgcgacagct tgatcacttc actgaaactg acgcctttcc
     1741 ttttctggga cagatgaagc atcttgatga tggcggggtt tgtttcatcg attccatcct
     1801 tgctcctccc gcttttttgg gcgacccaga tcgattggtt tcgctcgctg atcgattcga
     1861 cgaagtatgc ggaaaggcgt aatgactcaa ggtatttctc cctcagcgga agggtacgtt
     1921 tgacgacgat ccccccgttc agcttgaaga gatcggtgac aaactggttc gtcagcaggt
     1981 tgtcgccaat cgccatctcc atcaacattt gatccgcttg ggcgagcgca agatcgatca
     2041 aggcgcaatc gagaatgatg tcgcggtggt tggagacgta caagtatgct tgatcattcg
     2101 caagcttctc tgtcccgctg tgggtaaatg ccgttaccga tttttccacg atcttcggaa
     2161 gaaagaatcc ggcggtgatc gtgcgttgaa actcctcgta cgtggtgacg tggtcaagca
     2221 gggagacgat gtgtttcacc gactcctgaa gggcaaggcg ttcatcattc gtccgcggcg
     2281 gatcc
//
LOCUS       DAOPUZ010000197         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_50317_length_2278_cov_3.809267, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000197 DAOPUZ010000000
VERSION     DAOPUZ010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50317_length_2278_cov_3.809267"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKN79_03995"
     CDS             <1..415
                     /locus_tag="PKN79_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNZ94958.1"
                     /translation="IERHSDDFILIIGQYIDQLEEVAELFELPIITGSTPNAKRDELY
                     SAFRSGEIRILVVSKVANYAIDLPDASVAIQISGTFGSRSEEAQRLGRILRPKGRSSF
                     FYSVITRYSSEEEFSANRQKFLAEQGYSYEIEVWE"
     gene            412..2274
                     /locus_tag="PKN79_04000"
     CDS             412..2274
                     /locus_tag="PKN79_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94959.1"
                     /translation="MSHEQLKTLLEQYTDDEYFFLARNYLGLVESPFHKEHISSRLIG
                     FFALEENRKKMLSLIDSFDQMIITALLFTGGLRFEQLATLLEGIHPYGKLLLRVANLR
                     ERMIIIATGNTLVPNPLLEEHFYRFAGLTPLFGVEEGTVHTHPFSYREFLQAILTLAE
                     RPGRSFFRKEYETLFPTMRSDDLPSIFETIHSSFVKLGIIIEDPQRRVDWQRAHDLFL
                     LDEQQIKAMMLAGHMEGALNTCCADLVFLLEDIGTFSASGLKRLSQALAFKHHVKRCD
                     LTSVLLGLGLLVKTKGGYNHVSYTQGEPASSFIVDSDYSVSYLGSLDADSLLVRFAAL
                     ETLDRTRLWRITEERLRSALDGGVGYDEIRSYLKTYVNGTLNTALLKSLDLIAERSRQ
                     VQIYDGLVLHTDERVGRIVEQLPALQEHILDRLSPTIFLMKRSHERIWRQILTQAGLS
                     LGSTKGELAHGSDGETRELPALQWPKEFQKPKKFKKSVTLPPVVPDEKLKEAIIRKNY
                     PKEVEEDLLERFEERLIINKSQITPQILYTRIEAGGFDYQGKISLAKQASSKEGTVVQ
                     LFMGGEELMALALELTYTPKKEALFKVAILPQMETRVIPLSKIFLLRQIKMLFL"
BASE COUNT          541 a          657 c          578 g          502 t
ORIGIN      
        1 catcgagcgt cacagcgatg atttcatcct gatcatcggc caatacatcg accagctcga
       61 agaggtggca gagctcttcg agctcccgat catcacggga agcaccccaa acgcgaagcg
      121 ggacgaattg tatagtgcgt tcagaagcgg ggagattcgg atcctcgtcg tcagcaaggt
      181 cgccaactac gcgatcgatc tgcccgacgc ctcggtcgcc attcagatct ccggaacgtt
      241 cggctcccga agcgaagagg cgcagcgcct cgggcgaatt ctccggccca aaggccgatc
      301 gtcattcttc tattcggtga tcactcgcta ctccagtgag gaagagttca gcgcaaatcg
      361 gcaaaagttt cttgccgaac agggctacag ctatgaaatc gaggtgtggg aatgagtcat
      421 gaacaactga agacactcct tgagcagtac accgatgatg aatacttctt tttggcccga
      481 aactatcttg gcctcgtcga gtcgccgttt cacaaggagc atatctcttc tcgactgatc
      541 ggcttctttg cgcttgaaga gaaccggaag aagatgcttt ccctcatcga ttccttcgat
      601 caaatgatca tcaccgccct gctcttcacc ggaggcttgc gctttgagca gctggcgacc
      661 cttctggagg gaatccatcc atatggcaaa ctgctccttc gggtcgcgaa tctgcgcgaa
      721 cgcatgatca tcatagcgac aggcaatacg ctggttccca atccgctctt ggaagagcac
      781 ttctaccgat ttgccggttt gacacccctg ttcggagttg aagagggcac ggttcatacc
      841 caccccttca gctatcgcga attccttcag gcgatcctca cccttgccga acgaccgggc
      901 cgctccttct tccgaaaaga gtatgaaacg ctctttccga cgatgagaag cgatgatctt
      961 ccctccatct ttgaaacgat ccacagcagt ttcgtcaagc tcggaatcat catcgaagac
     1021 cctcagcgcc gggtcgattg gcagcgggct cacgacctct ttcttctgga tgaacagcag
     1081 atcaaggcga tgatgctcgc cgggcatatg gagggcgccc tgaacacctg ttgtgctgat
     1141 ttggtctttc ttttggagga tataggaacg ttttccgctt ccggcctgaa gcgcctgagc
     1201 caagccctcg ccttcaagca ccatgtcaag cgctgcgacc ttacctcggt gttgctcggt
     1261 cttggactgt tggtcaaaac gaaaggcgga tacaatcatg tctcctacac ccaaggggag
     1321 ccggcctcct ccttcatcgt ggacagcgac tattcggtct cttacctcgg gtcactggac
     1381 gccgactcgc ttctcgttcg ttttgccgcc ttggaaaccc tcgatcgcac ccgattgtgg
     1441 cgaatcaccg aagaacgact gcgcagcgcc ttggacggag gggtcggcta tgatgagatc
     1501 aggtcatact tgaaaacata cgtgaacgga acgctgaaca cggcgctgct caaatccctt
     1561 gatctgatcg ccgaacggag caggcaggtt cagatctatg acggccttgt cctgcacacc
     1621 gatgaacggg tcggccggat cgtcgaacag ctgccggcgc ttcaagaaca catcctcgac
     1681 cgtctttctc ccacaatctt cctgatgaaa cgctcccacg agcgaatctg gcgacagatt
     1741 ctgacccagg cgggcttgag tctcggttca accaaaggcg aacttgccca tggaagcgat
     1801 ggggaaaccc gggagctccc cgcccttcaa tggccgaagg agtttcaaaa gccaaagaag
     1861 ttcaaaaaat cggtaacgct tcctcccgtt gtaccggatg agaagctcaa agaggcgatc
     1921 atcaggaaga actatcccaa agaagtggag gaggatctcc tggagcgctt tgaagagcgc
     1981 ttgatcatca acaaatccca aatcaccccc cagatcctct atacaaggat tgaagcgggc
     2041 ggttttgatt atcagggaaa aatctccctt gcgaaacaag cctcctcgaa ggaaggaacc
     2101 gtcgtccagc tcttcatggg gggcgaggaa ctcatggcct tggcgctgga gctgacctac
     2161 acacccaaaa aggaagcact ttttaaagta gcgattctcc cgcagatgga aacccgggta
     2221 atcccgctct ccaaaatctt cctgctccgc cagataaaga tgctctttct ttaaagat
//
LOCUS       DAOPUZ010000198         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_50362_length_2277_cov_2.729523, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000198 DAOPUZ010000000
VERSION     DAOPUZ010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50362_length_2277_cov_2.729523"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKN79_04005"
     CDS             complement(<1..341)
                     /locus_tag="PKN79_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNZ94960.1"
                     /translation="MATQRREVSGQRLWGLIQEYFPNAQDLVGSIAVINYCPLVFVDR
                     GSTGKNITPDRLGLGERMALEAICDSYLHDMLTMLQPAFALGVGKYAENKLITVAQAF
                     DDITVGSILHP"
     gene            complement(367..651)
                     /locus_tag="PKN79_04010"
     CDS             complement(367..651)
                     /locus_tag="PKN79_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94961.1"
                     /translation="MNLFSNAIVSRTGDFANQMDSLRFNCDCYIYNPLDYAWPMMETY
                     IRRYLSRPVKTVFLGMNPGPFGMAQTGIPFGEITVVKEYLQIEEDRKTAG"
     gene            885..>2277
                     /locus_tag="PKN79_04015"
     CDS             885..>2277
                     /locus_tag="PKN79_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ94962.1"
                     /translation="MNHSWDVLDQYRGGLFEGTWPAILDLLSITKEKFPKRNGFTVFN
                     PERTTLTWEEVWSEVRRLGSYLIEAGIKADDKVVINGKNSPAWALSYFAALWAGAVVV
                     PLDNQMPIDRVHALGEFVEASFVFADAAIINQLNKEAPWVKNLKGIVSLTSPTATAVN
                     YTTLRPKKMVERVKRTEHDLAAILFTSGTTGNEKGAMLTHGNLVSDVFQACDGIFLQI
                     SEVDVLYALLPLHHSYSCTAVLLESITHGSECVFGHDIVVSKMINDMKRGKITVFMGI
                     PLLYNKILAGMMKQVRKKGVATYALIRTMMKVNGFFKKHLKWNPLRKSFDKLLLSKLG
                     LDHNRICICGAGPLSPKVFTQYQQLGIDFIQGYGLTETAPILTLNPVSRFKVESVGLV
                     FPLVDMIIADKDGSGIGEVRVKGPNVTSGYYKDPEHTAELFDEEGYLKTGDLGYLDKE
                     NYLYLKGRVKNVIV"
BASE COUNT          546 a          648 c          575 g          508 t
ORIGIN      
        1 ctcgggtgca ggatcgagcc gacggtaata tcgtcaaacg cttgggcgac ggtaatcagt
       61 ttattctcgg catacttccc caccccgaga gcaaaggcgg gctgtaacat tgtgagcata
      121 tcatgcaggt agctgtcgca gatcgcctca agagccatcc gctcccccag accgagtcga
      181 tcgggggtga tgttcttgcc ggtactcccc cgatccacga agaccagtgg acagtagttg
      241 atgacggcga tcgagccgac gaggtcttgg gcattgggga aatactcctg aatcaatccc
      301 caaagccgtt gcccgcttac ctccctgcgc tgagtcgcca atccgagaac cgggcgcttg
      361 gggtgctcaa ccagcggtct tccgatcctc ttcgatctgc aagtattcct tcacgacggt
      421 tatttcgccg aacggtatcc ccgtctgggc catcccgaag ggcccggggt tcattcccag
      481 aaaaaccgtt ttcaccggcc gggaaagata ccttcggata tacgtttcca tcatcggcca
      541 ggcataatcc aaggggttgt aaatataaca atcacaattg aagcgcaatg aatccatctg
      601 attggcgaaa tctccggttc gactgacaat cgcattactg aacaaattca catccccttc
      661 ctccacccct tgccataaaa tactcttcaa ccttggcata aaacgcttca aagctcaagg
      721 cggcccgttt tgccctaagg agacatcgac cgtttgagct ctagtacttt tctactatcg
      781 ctcgatggaa gtgcttggat tcttgacaaa gatacagact cttgtacact taaacccaga
      841 caatcactag tagttttata ctagtggaga tgcaggagct catcatgaac cattcctggg
      901 atgttctgga ccaatatcgc ggcggcctct ttgaagggac gtggcctgcc attctcgact
      961 tgctctctat cacgaaagag aagtttccca aacgcaacgg gtttaccgtg ttcaaccccg
     1021 aacgcacgac gctcacctgg gaggaggtgt ggtcggaggt ccggcgcctc ggctcgtatt
     1081 tgattgaagc ggggatcaag gccgatgaca aggtggtcat caacggaaag aactcccctg
     1141 cctgggcgct ctcctacttt gccgccctct gggccggggc ggtggttgtc cccctggaca
     1201 atcagatgcc gatcgaccgc gtccatgccc tcggcgagtt cgtcgaagca tcctttgtct
     1261 tcgctgatgc ggcgatcatc aaccagctga ataaagaagc gccttgggta aagaacctga
     1321 aaggaatcgt ctcccttacc agtcccaccg ccactgccgt caactacaca actcttcggc
     1381 cgaaaaagat ggttgaacgg gtcaaacgca cggaacacga tttggcggcg atcctcttca
     1441 ccagcggaac gaccggcaat gaaaaaggag caatgctcac ccacggcaac ctcgtcagcg
     1501 acgtgtttca ggcatgcgac ggaatctttc ttcagatcag tgaagtggat gtcctgtatg
     1561 cgctgcttcc ccttcatcac tcctacagct gcaccgccgt cctgctcgag tcgatcaccc
     1621 atgggtcgga gtgcgtcttc gggcacgata tcgtcgtcag caagatgatc aacgatatga
     1681 agaggggcaa gattaccgtc ttcatgggaa ttcctcttct ctataacaaa atccttgccg
     1741 gaatgatgaa gcaggtaaga aagaaagggg ttgcgaccta tgccctcatc cggacgatga
     1801 tgaaggtcaa cgggttcttc aagaagcatc tcaaatggaa tcccctgcgc aagtcgtttg
     1861 acaagcttct gctctccaag ctcggactgg atcacaaccg gatctgcatc tgcggagcgg
     1921 gcccgctttc tccgaaggtg tttacccaat accagcagct tggaatcgat ttcatccaag
     1981 gctacgggct caccgaaacc gctccgattc tcaccctcaa ccccgtctcc cgcttcaaag
     2041 tcgaatcggt cggattggtc tttcccctcg ttgatatgat catcgccgac aaggacggct
     2101 cggggatcgg cgaagtccgg gtaaagggac cgaacgtaac ttccggatac tacaaggatc
     2161 ccgaacatac cgccgaactc ttcgatgaag aggggtatct gaagacgggc gacttgggct
     2221 atctggacaa ggagaactac ctctacctca aaggcagagt gaagaacgtg atcgtta
//
LOCUS       DAOPUZ010000199         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_50682_length_2268_cov_3.105287, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000199 DAOPUZ010000000
VERSION     DAOPUZ010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50682_length_2268_cov_3.105287"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKN79_04020"
     CDS             complement(<1..637)
                     /locus_tag="PKN79_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNZ94963.1"
                     /translation="MKKLITILLMVALIAPVFAAGGGEKAPAKTTINFPTAATTGAVY
                     PLGAAISNMWNNKVPTVRAAAQASAGGIENLNLIADGEAQVGVAVTSIMYESFTGTGT
                     FEGRANPNLRVMIGLYYNPNQVVVAKNSNINSLNDLAGKSFASGAPGSTTEVETAIHL
                     KESGINYPDALKVQYIGFTEAIDLMRNKQIDGAWIMAGIPNAAVTEMLSTAG"
     gene            complement(634..>2268)
                     /locus_tag="PKN79_04025"
     CDS             complement(634..>2268)
                     /locus_tag="PKN79_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HNZ94964.1"
                     /translation="LLLYYTTVAKRGGYLNKTDLVVAFILLAVIFEAGRRACRSLMYL
                     GLIFLLYNFLGRFIPGDLGHVGFSLKRVLSHMVWGSQGVFGVGIGVSATYIFLFVLFG
                     AYLKYSGFSQFINDVSLTLVGRSAGGPAKVAVIASALMGMINGSAIANVATTGTITIP
                     MMKKTGYKKEFAAAVEAVASTGGQFTPPIMGAVGFVMAEFMGVSYTKVLVAAAIPAFL
                     YYLSLLFAVHFEAKRLGLSGLSKENIPDALEVFKKQGHLLIPLIVLLVLLGIGYTPLF
                     AAVISIFATVGASWLRKETRMDWRTIVQATVEGARSSITVGMSCVIIGVIIGTVSLTG
                     LGLSFGFVILRIVGEGQLFLGGLMVMIMSTVLGMGVPGVAAYVIVSAVSVPVLIQTGA
                     IPMAAHMFCLIYACLSNITPPVAMSSYVASGIADSNQTKTSLIAVKLGLTGFILPFFF
                     LNNPILLIGSTPDVSVLVTIRAVITSSIGAVALSAGLQGYLLRNLAIIERGLLIISGL
                     LFIEPGLITDLIATGILITLLLLQYFQTYRMKERII"
BASE COUNT          506 a          637 c          681 g          444 t
ORIGIN      
        1 cgcctgcggt ggagagcatc tcggttaccg ccgcgtttgg aatgcccgcc atgatccaag
       61 caccgtcgat ctgcttgttg cgcatcagat cgatcgcctc ggtgaagccg atgtactgga
      121 ctttcagcgc atccggatag ttgatccccg attctttgag gtggatcgcc gtctcgactt
      181 cggtcgtcga accgggagcg ccgctggcaa aggatttgcc tgcaaggtca ttgagggagt
      241 tgatgttcga gttcttggcg acgaccacct ggttcgggtt gtagtagagt ccaatcatca
      301 cccgaaggtt ggggttggcc cgaccttcaa aggtgccggt tcccgtaaag gactcgtaca
      361 tgatcgaggt gacggcgaca ccgacctgcg cttcgccgtc ggcgatcagg ttgaggttct
      421 cgattccgcc ggcggaagcc tgggcggcag cccggacggt gggaaccttg ttgttccaca
      481 tgttcgagat tgccgcaccc agcgggtaca cggcgccggt ggttgctgcg gtcgggaagt
      541 tgatcgtcgt cttcgcgggg gctttttcac cgccgccggc ggcaaagacg ggggcgatca
      601 gggcaaccat caaaagaatt gtgatgagtt ttttcatata atacgctcct tcatacgata
      661 tgtttggaaa tattgcaaaa gcaagagagt aatcaagatg cctgttgcga tgaggtcggt
      721 aatcagaccc ggctcaatga acagcagtcc gctgatgatc agcaagcctc gttcaatgat
      781 tgccagattc ctcaggagat acccctgcag acccgctgaa agggcgacgg ctccgatcga
      841 agaggtgatg accgcccgga tcgttacgag aacggaaaca tccggagtcg agccgatcaa
      901 cagaatcgga ttgttcagga agaagaacgg gagaataaaa ccggtcaagc caagcttcac
      961 cgcgatcaga cttgttttgg tctggttgga atcggcgatg ccgctggcca cgtagctgct
     1021 catcgcaacc ggcggggtga tattggaaag acacgcatag attaggcaga acatgtgggc
     1081 cgccatcgga atcgccccgg tctggatgag cacgggaacg ctgactgcgg agacgatcac
     1141 atacgcggcg acaccgggaa ctcccattcc cagaacggtg ctcatgatca tcaccatcag
     1201 gccgcctaag aagagctggc cttctcccac gatcctcagg atgacgaagc cgaagctgag
     1261 ccccaggccg gtaaggctca ccgtgccgat gatgactccg atgatgacgc agctcatccc
     1321 cacggtgatg gaagagcggg ccccttcgac cgtggcctgt acgatcgtcc tccaatccat
     1381 cctcgtctct tttctcaacc acgaggctcc gacggtggcg aagatcgaga tgaccgcggc
     1441 aaagagggga gtgtatccga ttcccaggag caccagaagg acgatcaggg gaatcagaag
     1501 gtgtccctgc tttttgaaga cctcaagtgc atcggggatg ttctccttgc tcaggccgga
     1561 aaggcccagc cgtttcgcct cgaagtggac ggcgaagaga aggctgaggt agtagagaaa
     1621 ggcggggatc gcggcggcga ccagaacctt ggtatacgag acccccatga actccgccat
     1681 gacgaatccc accgctccca tgatcggggg agtgaactgt cctccggtgg aagccaccgc
     1741 ttcgacggcg gcggcaaact ccttcttata gccggttttc ttcatcatcg gaatcgtgat
     1801 cgtcccagtg gtcgccacgt tggcgatcgc gcttccgttg atcatcccca tcaacgcgct
     1861 ggcgatgacc gccaccttgg ccggtccgcc ggcggatcgt ccgacgaggg tcagagagac
     1921 atcgttgatg aattggctga agccggaata tttcagatac gccccgaaca ggacgaacag
     1981 gaagatatag gtggcgctga ccccgattcc caccccgaag actccctggc ttccccagac
     2041 catgtggctg aggacccgtt tcaagctgaa gcccacgtgc cccaggtcgc cggggatgaa
     2101 gcggccgagg aagttgtaga gcaggaaaat caatcccaga tacatcaggg accggcatgc
     2161 ccttcgcccc gcttcgaaga tgaccgcaag gagaatgaac gcgacgacca gatcggtctt
     2221 gttcagatac ccgccccgct tggcgaccgt ggtgtagtac agcagaag
//
LOCUS       DAOPUZ010000200         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_50863_length_2263_cov_3.391757, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000200 DAOPUZ010000000
VERSION     DAOPUZ010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50863_length_2263_cov_3.391757"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..587)
                     /gene="scpB"
                     /locus_tag="PKN79_04030"
     CDS             complement(24..587)
                     /gene="scpB"
                     /locus_tag="PKN79_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNZ94965.1"
                     /translation="MSDAIRSLSEDARLLEVILFLENEPVSVEKFVKMTDLNEDEVME
                     ALAEIEEYYDTYRHGMSLFENHGAFQFLPSADLHERLRSCYGRRVDRRLSRAAMETLS
                     IVAYSQPITRREIDNIRGVSSDSIIRLLRDREYIKVVGRKDVPGHPCLYGTSRKFLFE
                     FNLNSIAALPKLSEIDQERFEVNEEEA"
     gene            complement(584..1525)
                     /locus_tag="PKN79_04035"
     CDS             complement(584..1525)
                     /locus_tag="PKN79_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNZ94966.1"
                     /translation="MERTEQQVSENLGLVYTTAGFEGPLGLLLHLIQKAEVNIYDIPI
                     AEITEQFIAYLESEESVLLGDLTEFYKMAADLLYIKSRMLLPVDLEFDEEYQDPRQEL
                     VERLLEHQRFKKYTDLLSGTATSGELFISRKPSQFRLPFGDEELFGEVTLDDLLQTFR
                     KLIKQIAPSKVFNVYESVTVNEKIALMQEVFETREYMTIEELIVDPTSRLHIICAFMA
                     ILDATKQGMIYLLQERAFETIVIRRRNEYFEEDYSDAYDEDEEYIPAQIEPLPISNEE
                     EAAFYREDVIHGDDDERELIFDGDVEEIVFDDEEDEL"
     gene            complement(1529..>2263)
                     /gene="recA"
                     /locus_tag="PKN79_04040"
     CDS             complement(1529..>2263)
                     /gene="recA"
                     /locus_tag="PKN79_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNZ94967.1"
                     /translation="DAEHAMDPSYAKKLGVNIDELWISQPDSGEQALEITESLVRSGA
                     IDMIVIDSVAALTPQAEIDGDMGDSHMGLQARLMSQALRKLTGLLSKSNTTIIFINQI
                     RMKIGVMFGNPETTTGGNALKFYSSVRIDVRKIETLSRGADDVYGNRIRIRIVKNKVA
                     PPFKKVELDLLFGEGISYEASLLDAALKYEMIDKAGSWYSYKDEKIGQGRDRTIEFLK
                     SNPDILADLDKRLRETMFGHSNVEKE"
BASE COUNT          476 a          635 c          578 g          574 t
ORIGIN      
        1 ctggaggcga accggatact cgatcatgct tcctcctcat tgacttcaaa gcgttcctga
       61 tcgatttcac tcaatttagg caacgctgcg atcgaattca aattgaattc aaataaaaac
      121 ttccgactcg ttccatagag gcacggatgg ccggggacat ctttgcgtcc gacgactttg
      181 atatactccc gatcacggag aagccgaatg atcgagtcgc tggaaactcc ccggatatta
      241 tcgatttcgc ggcgggtgat cggctggctg taggcgacga tcgagagcgt ctccatagcg
      301 gcccggctga gccggcggtc gacccgtcgc ccgtagcagg aacgaagccg ttcatgaagg
      361 tccgccgaag gcaggaactg gaaggctcca tggttttcga agagactcat gccatgccgg
      421 taggtatcgt aatactcttc gatttcagct agagcttcca tgacttcatc ttcgttcaga
      481 tcggtcattt tcacgaactt ctcgacgctg accggttcat tttccaaaaa caggatcacc
      541 tccaacaggc gggcgtcctc actgagggat cggattgcat cactcacaat tcatcctcct
      601 catcatcaaa cacgatttct tccacatctc cgtcaaatat aagttcccgt tcgtcatcat
      661 cgccatggat cacatcctcc ctgtagaaag cggcttcctc ctcattgctg atcggcaggg
      721 gctcgatctg agccgggatg tactcctcat cctcgtcata cgcatcggag taatcctctt
      781 caaagtactc gttccgtctt cgaatgacga tcgtctcgaa cgcccgctcc tgcaggaggt
      841 agatcattcc ctgtttggtg gcatccagga tcgccatgaa ggcgcagatg atgtgaagtc
      901 tgctggttgg atcgacaatg agctcttcga tcgtcatata ctcccgggtt tcaaagacct
      961 cctgcatcaa ggcgatcttt tcattgaccg ttaccgactc atagacgttg aacaccttcg
     1021 acggggcgat ctgtttgatc agttttcgga acgtttgtag aagatcgtcg agggtcacct
     1081 caccgaagag ctcctcatcc ccgaacggca ggcggaactg gctcggtttt cgggagatga
     1141 agagttctcc gctcgtggcg gttccgctga gcaggtcagt gtacttcttg aagcgctggt
     1201 gctccaagag gcgttcgacc agctcctgac ggggatcctg atactcttca tcgaactcca
     1261 gatccaccgg aagaagcatt cggctcttta tgtagagcag atccgccgcc atcttgtaga
     1321 attccgtcaa atcgccgaga agaaccgatt cctcgctctc cagataggcg atgaactgct
     1381 cggtgatctc ggcgatcgga atgtcgtaga tgttgacttc ggctttctga atcagatgaa
     1441 gcagcaatcc gagcggacct tcaaagcctg cggttgtata caccaagccg agattctcag
     1501 aaacctgctg ctccgttcgt tccacgttct actctttttc gacgtttgag tgcccaaaca
     1561 tcgtttccct gaggcgtttg tccaaatcgg caaggatatc ggggttcgat tttaagaact
     1621 cgatcgttcg atcccggcct tggccgatct tctcatcctt gtaggaatac cacgatccgg
     1681 ctttgtcgat catctcgtat ttgagagcgg cgtccagcag gctcgcttca tagctgattc
     1741 cctccccgaa gaggagatcc agctccacct tcttgaacgg cggagccact ttgttcttca
     1801 caatccggat tcgaatccgg tttccatata catcatccgc tcccctgctg agggtttcga
     1861 tcttgcggac atcgatgcgg accgaggagt aaaacttgag ggcgtttccg ccggtggtcg
     1921 tttcggggtt cccgaacatc accccgatct tcattcggat ctggttgatg aagatgatcg
     1981 tcgtgttgga tttgctcaag agaccggtga gtttgcggag agcctggctc atcaggcgcg
     2041 cctgaagacc catgtgactg tcgcccatgt ctccgtcgat ctccgcctgg ggggtaagag
     2101 cggccacgct gtcaatgacg atcatgtcga ttgcgccgct gcggaccagg gactcggtga
     2161 tttcgagcgc ctgttcaccg ctgtccggct ggctgatcca gagttcgtcg atattcaccc
     2221 cgagcttctt cgcataggag ggatccatcg catgctcggc atc
//
LOCUS       DAOPUZ010000201         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_50991_length_2259_cov_3.216425, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000201 DAOPUZ010000000
VERSION     DAOPUZ010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50991_length_2259_cov_3.216425"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKN79_04045"
     CDS             complement(<1..208)
                     /locus_tag="PKN79_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94968.1"
                     /translation="MKKIVLLVAMATILMVGTSCATSKKAAVVTPPVPAAKVVVGAEG
                     FIQPDWVYKTPTAGDRHFESGYGLM"
     gene            complement(227..1180)
                     /locus_tag="PKN79_04050"
     CDS             complement(227..1180)
                     /locus_tag="PKN79_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94969.1"
                     /translation="MMRKGVLLFFLIPFLVSCMSIPNQRPAWIGRPYDGKYPEDKYLL
                     VVGSGSSYQKAVENARINLASVFSSQVEAVTRITTIDSSEGGSFDALFEQGTVSSNVE
                     DLVGSEVVSSFTDEMGQTYVRLAVERQESARFLQTRLAELTKELAKIRSQTFADRLER
                     YRFLLSAYPVAQEAGQIADRIWVLTKEINPDHLGALASELRTLARSISVEVLVHSETD
                     PHFLKAGFEGALQNLGFVQGGGDYALTVTYDSEPILFEASPYAYERYQLSVTLGRNDK
                     VVRSFSLSERIAALHQGDAKTKALQSALNDGIETLIGLLSQ"
     gene            complement(1190..1807)
                     /locus_tag="PKN79_04055"
     CDS             complement(1190..1807)
                     /locus_tag="PKN79_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HNZ94970.1"
                     /translation="MRRSGIIIISIIMLLSLALLTACQNSYLVSRVSSDKDLDLSGNW
                     NDTDIRLVSKALVESSLKSGWINQFRMKNVGRNPVVIVGTFFNRSSEHIDTAIIAKRY
                     EMELINSGKVDMVADQNFRASVRQEREEQQYFASEESAKALGREIGADFLLQGAVRSV
                     LDQRGNTRVRTYYVSAELIDIETNLKVWVGEETIKKVIQQARYTL"
     gene            1923..>2259
                     /locus_tag="PKN79_04060"
     CDS             1923..>2259
                     /locus_tag="PKN79_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94971.1"
                     /translation="MDIRKIIPVLLAIILATSCVSYVDLSSTDAYLKRGQYTKAYDSL
                     QKTIGHLATAQGPLIASYDLGMLARLTGDWKHSNELLSDAERRIQDAYTKSITASLAS
                     FIVNDNTKEY"
BASE COUNT          528 a          631 c          578 g          522 t
ORIGIN      
        1 tcatcagccc atacccgctt tcaaagtgcc gatcacccgc ggtcggggtt ttgtataccc
       61 aatccggttg gatgaaccct tcagctccga caacgacctt ggccgccgga acggggggcg
      121 tgacgacggc tgcttttttg ctcgtggcac acgaagtacc aaccatgagg attgttgcca
      181 ttgcgacaag aaggacgatt tttttcataa cgctgtacct ccatttttac tggcttaaaa
      241 gtccaatgag tgtttcgatt ccatcgttca gggcgctttg aagcgctttg gtcttggcat
      301 ccccctgatg aagggcggct atccgctcgc tcaggctgaa ggatcgaacc accttatcgt
      361 tccgcccgag ggtaacgctc agctgatagc gttcgtaggc ataggggctg gcttcaaaga
      421 ggatcggctc cgaatcatag gtgaccgtca gggcatagtc cccgccgcct tgaacaaacc
      481 ccagattctg aagcgcccct tcgaatcccg ctttcaggaa atgcggatcg gtttcgctgt
      541 ggacaaggac ctcgaccgag atcgaccgag cgagggtgcg cagttccgat gcgagggccc
      601 cgaggtggtc tggattgatc tctttggtca acacccagat tcgatcggca atctgtcccg
      661 cctcctgcgc caccggatac gcggaaagca ggaagcgata gcgctccaat cgatcggcga
      721 atgtttggga gcggatcttc gcaagctcct tcgtcaattc agccaatcgg gtttgaagaa
      781 atcgggcgct ctcttggcgc tcgaccgcga ggcggacgta ggtctggccc atctcgtcgg
      841 taaaggagga aacgacctcc gatccgacaa gatcctcgac gttcgaagag accgtcccct
      901 gctcaaagag cgcatcgaag ctcccgccct cgctcgaatc gatcgtggtg atccgggtaa
      961 cggcttccac ttgggaggaa aacaccgagg cgaggttgat ccgggcgttt tcaaccgctt
     1021 tctgatagga ggagccgctg cctacgacca gcagatattt atcctccgga tacttcccgt
     1081 catagggtct gccgatccag gcaggccgct gatttggaat cgacatgcag gaaacaagaa
     1141 aaggaatgag aaaaaaaaga agaacgcctt ttctcatcat caccatcctt tagagggtat
     1201 accgagcctg ctgaatcact ttcttgatcg tttcctcgcc aacccaaact tttaggtttg
     1261 tttcaatatc gatcagttca gcggatacgt aataggttcg aacccgagtg tttccccgtt
     1321 gatccagaac gctcctcacc gcgccttgga gcaggaaatc ggctccgatc tcccggccaa
     1381 gcgcttttgc actctcctca ctggcgaaat actgttgctc ttcccgctcc tgacgaaccg
     1441 aagctcggaa gttttggtcg gcgaccatgt cgactttgcc gctgttgatc agctccatct
     1501 cataccgctt ggcgatgatc gccgtgtcga tgtgctcact ggaccggttg aagaatgtcc
     1561 cgacgatgac caccgggttt cgcccgacgt ttttcatcct gaattgattg atccacccac
     1621 ttttcaagga ggattcaacc aatgccttgg aaacaaggcg aatatccgtg tcattccagt
     1681 tcccgctgag atccagatcc ttgtcgctgc tcactctcga caccaaatag gagttttgac
     1741 aggcggtaag aagagcgagg gaaagaagca tgatgatact gataatgata attccgctac
     1801 ggcgcatacg atcctcctgc ttctgaaagt gtagcagaag aatgacgtat tggatagaat
     1861 catatcatca ataaattgca tccatgcccc gacaatgata cagttgaggg caaggaggag
     1921 cgttggatat ccgaaagatc atacccgttt tgcttgccat catccttgca acctcgtgtg
     1981 tctcctatgt cgatctgtca tcgaccgacg catacttgaa gcgcggtcaa tacaccaaag
     2041 cgtatgattc cctgcaaaaa accatcgggc acctcgccac cgctcaggga cccctgatcg
     2101 cgagctatga cctggggatg cttgcccggc tcaccgggga ttggaagcat tccaatgaac
     2161 tgctcagcga tgcggagagg cgaatccaag atgcgtatac gaaatcgatc accgcatcac
     2221 ttgcctcctt catcgtcaat gacaacacca aagagtatg
//
LOCUS       DAOPUZ010000202         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_51020_length_2258_cov_3.640036, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000202 DAOPUZ010000000
VERSION     DAOPUZ010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51020_length_2258_cov_3.640036"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKN79_04065"
     CDS             <1..308
                     /locus_tag="PKN79_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94972.1"
                     /translation="HIADLKYTTNSRTGVTLGIVGSELKSTENNVIPYTLTLGASTTI
                     KVGYAEDNNPEGTIPTPSQFTQLTTTTAEVHLTIPGSVSDYPAGAYTGTITFTLGAK"
     gene            complement(373..1494)
                     /locus_tag="PKN79_04070"
     CDS             complement(373..1494)
                     /locus_tag="PKN79_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94973.1"
                     /translation="MDIRRLKLFNSRNAALLTNDLVQLVVEDQGGMVLEFSAKIANGG
                     RINPHAIPSHRGTGSSVYSDENGEFWQNNPTLYQQAGSYLVFPKEDEHSTAASYWLVE
                     QYGTDPEFGGVWLTSRMRNRLEGWQIRKLDLLLPNQPVHYTAYQVTNKAPEPLRATAQ
                     HRTLLGPPFLEQGCVVNTNAASFESFPIPNTQSRIPAGQFNDWKKIQTRENGSLDLTE
                     IPKVTNTVDFIKVTLNQDAPLGFTSMINPRMQMAYVTFFPSKPEDEDELVFTHTSFLY
                     QLGGMSQTPYALYDGGLDQQYTVELGEGFLTTLEPLQTRIFFHARAFAPYENARLGGN
                     FHSLEQTLEGILLKRTKTTALIPADPSFHAARTLAKRLF"
     gene            1542..>2258
                     /locus_tag="PKN79_04075"
     CDS             1542..>2258
                     /locus_tag="PKN79_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HNZ94974.1"
                     /translation="MITNKDRLFNLSTKRTTYLFAVTETGHLEHLYWGRRLTKDVSID
                     AFAEKRSIGIIGETAYDSDHPTLFLSNLAMEYSTTGKGDYRESSIDIEYQKGLRTLDF
                     TLQSFRILKGKPRLFGGLVESYGDHEQCTTLEITLREKLLPLRILLTYTTFEDTDVIT
                     RRTTLHNDGHESVVIRNLASAQVDLEGSWELITFDGAWGRERYVNRRELKPGIYINDS
                     KKGVSSSDHNPAIFLTNEENE"
BASE COUNT          534 a          676 c          600 g          448 t
ORIGIN      
        1 atcatatcgc cgacctcaaa tataccacca actccagaac cggggtgact ctgggaatcg
       61 tcggaagcga gctcaaaagc accgaaaaca acgtcatccc ctacacgctt accttgggag
      121 cgagcacgac gataaaggtg gggtatgctg aggacaacaa tccggaaggt acgattccga
      181 ccccctccca attcactcag ctgactacga cgaccgcaga ggtccatctc accattccgg
      241 gcagcgtcag cgactacccg gccggcgcct atacgggaac gatcaccttc accctcgggg
      301 cgaaatagcc gaatcttttt cacgtacttt tcaaagccct caatcagaag ggcttttctt
      361 tcgcctttag ccttagaaca atctctttgc cagggtcctt gccgcgtgga acgacggatc
      421 ggccggaata agggcggtgg tcttggtccg tttgagcagg atgccctcca gagtctgttc
      481 cagggaatgg aagtttccac ccaagcgagc gttctcatac ggggcgaacg cccgggcatg
      541 gaagaagatc ctcgtctgaa gaggctccag agtcgttaaa aacccttcac cgagctcgac
      601 cgtgtactgc tggtcgagcc cgccgtcata gagcgcatag ggagtctggc tcattccacc
      661 cagctggtag agaaacgagg tatgggtgaa gacaagctca tcctcatcct caggcttgga
      721 gggaaagaac gtgacgtacg ccatctgcat cctcgggttg atcattgagg tgaagccgag
      781 gggagcgtct tggttcaagg ttaccttgat gaaatcgacg gtgttggtga ccttagggat
      841 ctcggtgaga tccagcgaac cgttctccct ggtctgaatc ttcttccagt cattgaactg
      901 ccccgcgggg atccggctct gggtgttggg aatcggaaac gattcgaagc tcgctgcgtt
      961 ggtgttcacc acgcacccct gctcgaggaa gggagggccg agcagcgtcc gatgctgcgc
     1021 cgtagccctg agcggctcgg gagccttgtt ggtgacctga taggcagtgt agtggaccgg
     1081 ctgattgggc agaagcagat cgagcttccg gatctgccac ccttccaggc gattgcgcat
     1141 ccggctggtc agccacactc ccccgaactc gggatcggtg ccgtactgct cgaccagcca
     1201 ataggatgca gcggtcgaat gctcgtcctc cttggggaac accagatatg aacccgcctg
     1261 ctgatagagg gtcggattgt tctgccagaa ctcgccgttc tcgtcggagt acaccgaaga
     1321 acccgttccc cggtgggaag ggatcgcatg cgggttgatc cgcccgccgt ttgcgatctt
     1381 cgcgctgaac tccagcacca tcccgccctg gtcctcgacg accagctgaa ccaggtcatt
     1441 cgtcaagagc gccgcattgc ggctgttgaa cagtttcaaa cgacgtatat ccattcctaa
     1501 ctctccaaaa agacggtata ctctaggaca aggagcgaca gatgattacc aacaaagacc
     1561 gactcttcaa cctttcaacc aagcggacca cctacctctt cgcagtaacc gaaaccggac
     1621 accttgagca cctctattgg ggtcgtcggc tcaccaaaga cgtaagcatt gatgcattcg
     1681 ccgaaaagag aagcatcggc atcatcggtg aaaccgccta tgacagtgac catcccacgc
     1741 tgtttctctc caatctcgcc atggagtatt ctaccacagg caaaggagac tatcgagaga
     1801 gttcgatcga catcgaatac caaaaaggac tgagaaccct cgacttcacc cttcagagct
     1861 tcagaatcct gaaaggcaag ccccggctct tcggcggcct ggtcgaatcc tacggtgatc
     1921 atgaacaatg caccacgctg gagatcaccc tcagggagaa gctgcttccg cttcgaatcc
     1981 tgctgaccta caccaccttt gaagacaccg acgtgatcac ccgtcgcacg acgctccaca
     2041 acgacggcca cgagagcgtc gtcatccgca acctcgcctc cgcccaggtc gacctggaag
     2101 gttcctggga acttatcacc ttcgacggcg cctggggccg ggagcgatac gtgaacaggc
     2161 gggaactgaa acccgggatc tacatcaacg actcgaagaa aggcgtcagc tcttccgacc
     2221 acaaccccgc gatcttcctg accaacgaag aaaatgaa
//
LOCUS       DAOPUZ010000203         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_51241_length_2252_cov_2.766045, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000203 DAOPUZ010000000
VERSION     DAOPUZ010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51241_length_2252_cov_2.766045"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PKN79_04080"
     CDS             <1..461
                     /locus_tag="PKN79_04080"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HNZ94975.1"
                     /translation="SPMKKANFVVSGAFTLFALAIIFLSRSYPPSNHGVPGPRMFPLI
                     IATLIIISSVVLFINTLRMKPEDDVPVDLTSRNVLNVYITIAGLIVYITLLPLLGFIT
                     TTSIMLLLFFKWFSRYAWWKCLLISVLFTVGIWALFSIVLNVPMRYGVLI"
     gene            478..1956
                     /locus_tag="PKN79_04085"
     CDS             478..1956
                     /locus_tag="PKN79_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNZ94976.1"
                     /translation="MLLSVLSSVFNLKIFFFIILGVFTGISIGALPGLTATMGVALVL
                     PLTFGMDAIPGILLLIGVYVGAIYGGSISAILLRTPGTPAAAATILDGYEFSKRGESG
                     RALGISTVSSFIGGVVSVIALWLISPQLAKLALRFSAPEFFLLAVFGLSIIASISGNS
                     LAKGVLTGGLGISLAFVGIDGITGYPRFTFNNINLLSGVSFIPVMIGLFAMSQAFTTI
                     ETIFTPDQVTQKVSNVFPTKADWKVIFKHAPISGVIGTIIGIVPGAGAEIGAFVSYGQ
                     AKRMSKHPERFGTGIPEGIAAPEAGNNGVTGGALIPMLTMGVPGDAVAAILIGALTVQ
                     GLQPGPMLFAEHPVLVYSIFLGMFVANVAMLVLGLSSLRLFVKVLSVPKSILTPMIFV
                     LCVVGSYAINTSFFDVGVMLFFGVLGYFLQKADASVSPAVLGLILGPMAESNFRRALL
                     MSEGSYGIFLSSPISWIFLILTAITLLGPFVGAYLKKRKAQA"
     gene            2125..>2252
                     /locus_tag="PKN79_04090"
     CDS             2125..>2252
                     /locus_tag="PKN79_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ94977.1"
                     /translation="MAYKITDDCIACGTCLPECPTGAISEGDIYVIDADICIDCGV"
BASE COUNT          438 a          630 c          583 g          601 t
ORIGIN      
        1 aatcacctat gaaaaaagca aattttgtcg tcagcggtgc atttaccctt ttcgccctgg
       61 caatcatctt tttgtctcgt tcctatcccc catccaacca tggggttccg gggccgagaa
      121 tgttccccct cattatcgca acgttgatca tcatttcgtc ggtagtgctg tttatcaaca
      181 ccttaagaat gaagcccgag gatgacgtcc ctgtcgatct gacgagcaga aacgtcctga
      241 acgtgtacat tacgatcgcc ggcctgatcg tctacatcac cctgctgccc ctccttggct
      301 tcattacgac gacctcgatc atgctccttc tgttcttcaa atggttcagc agatacgcct
      361 ggtggaaatg cctgctgatc agcgtattgt tcacggtggg catctgggcg ctcttcagta
      421 tcgtgctcaa cgtgccgatg cgctatggcg tattgatata ggaaggaggt taaaaatatg
      481 ttgcttagcg tcttgagttc cgtcttcaat ctgaaaatct tcttttttat catccttgga
      541 gtgtttaccg gtatttccat cggagctctc ccgggtctta ccgcaacgat gggtgtcgcc
      601 ctcgtgctgc cgcttacctt cggaatggat gcgattcccg gcattctcct tttaatcggg
      661 gtatatgtgg gggcgatcta cggcggttcg atctccgcga ttctcctgcg cacccccggc
      721 acgccggcgg cggctgccac cattttggat gggtatgagt tctccaaacg gggtgagagc
      781 ggccgggctt tggggatttc gacggtctcc tcctttatcg gtggagtcgt cagcgtcatc
      841 gccttatggc ttatttctcc ccagcttgca aagctggctc tccggttcag cgcccccgag
      901 ttcttccttc tggcggtctt cgggctttcg atcatcgctt cgatcagtgg aaacagcctc
      961 gccaagggag ttctcaccgg aggattggga atcagtctgg cattcgtggg aatcgacgga
     1021 atcaccggat atccccgctt caccttcaat aacatcaacc tcttaagcgg ggtttcgttt
     1081 atccccgtca tgatcggact ttttgcgatg agtcaggcgt tcacgacaat tgaaacgatc
     1141 ttcactcccg atcaggtgac gcaaaaagtc agtaatgtgt ttccgacaaa agcggactgg
     1201 aaagtgatct tcaaacatgc gccgatcagc ggtgtcatcg gaacgatcat cggaatcgtc
     1261 cccggggcgg gagcggagat cggggcgttc gtctcctacg gtcaggcgaa acggatgagc
     1321 aaacaccccg aacgcttcgg aacgggaata cccgaaggaa tcgccgctcc ggaagcggga
     1381 aacaacggag ttaccggcgg agcgctgatt ccgatgctca cgatgggagt ccccggagac
     1441 gctgtcgccg cgattctcat cggagcgctc accgtccagg gacttcagcc cgggccgatg
     1501 ctttttgccg aacacccggt actcgtctat tcgattttct tggggatgtt tgttgcgaac
     1561 gtcgcgatgc tcgtcttggg actgtccagt ctccggctct tcgtgaaggt cctctcggta
     1621 cctaagtcga tcctcacgcc gatgatcttc gtcctctgcg tcgtcggctc gtatgcgatc
     1681 aacacgagct tcttcgatgt cggtgtgatg ctcttcttcg gtgttctcgg ctacttcctt
     1741 caaaaagcgg atgcctccgt ctctccggcg gtcctcggtc tcatcttggg cccgatggcg
     1801 gagtcgaact tccgccgcgc tctcttgatg agtgaaggca gctatgggat cttcttgtcc
     1861 tccccgatct cttggatctt cctgattctc acggccatca ctcttctcgg tccgtttgtc
     1921 ggtgcctatc tgaagaagcg gaaagcccaa gcgtaacaat catcgattcc aacaaacgca
     1981 agtcacctcc gggtggcttg ttttttcatc gccctccaat cgtatattcg aggcaccgaa
     2041 ggtttctttt ttttctacct cttgttgact agaaccaaag gggggtatat agttttgaca
     2101 acgataccaa tcaaggagct ttccatggct tacaaaatca cggatgactg catagcttgc
     2161 ggaacatgtc taccggagtg cccgaccggg gcgatcagcg aaggcgatat ctatgtgatc
     2221 gacgccgaca tctgcatcga ctgcggtgtc tg
//
LOCUS       DAOPUZ010000204         2246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_51442_length_2246_cov_3.584208, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000204 DAOPUZ010000000
VERSION     DAOPUZ010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51442_length_2246_cov_3.584208"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PKN79_04095"
     CDS             <1..902
                     /locus_tag="PKN79_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNZ94978.1"
                     /translation="LFSYLFLISLAGTMSSGYAMKLLKSLLKERVSLIGISVVGAFVS
                     NLSQLQVASWIVYGPAIWVAAPLMLGLGMITSIILGVFGERYLRHGSFPAAFLADSFE
                     PVLPTAEERAPLVERTVALIIAIGAILFAKGPIPLLGIAVLMYVIQGISGRKIRLFPP
                     LMLLSSLIVLSLFEPNGQVIVTVGTLALTKGSIALALTKGLRLIALLSASQSLVSSNP
                     RLKGRLGSLLVLALSYFGILTSCFRTAKGSLPQRIDGALFCAAEGSLQVSANNQQERS
                     SSALIIITAILVVGISIASRVWG"
     gene            945..1751
                     /locus_tag="PKN79_04100"
     CDS             945..1751
                     /locus_tag="PKN79_04100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HNZ94979.1"
                     /translation="MPSIKLICTDIDGTLLRPDHTISPRTKRAIQEAHKRGIIVALVS
                     GRIGKSLVKIQEELGITGPLGTLNGSLVLDEKGRSILEHPLKKDEIHQILEWAKQTDL
                     NAFVYTNTNWYASTEGYWTEYELKISGFKGIVRSFHDHEGRLGADEKPFKVLCMDKDP
                     EYLSKMERQLIQQMGSRFTILRSGPIYLEIFHRGVDKGDAVKALGSHYGIPREAIMAV
                     GDYYNDVAMLKAAGYSVAMGNAPDDIREMADVVTRPNTEDGLALAIESIL"
     gene            complement(1746..2174)
                     /locus_tag="PKN79_04105"
     CDS             complement(1746..2174)
                     /locus_tag="PKN79_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HNZ94980.1"
                     /translation="MCLFVCPTGATDTESGQIDASKCLDGCRLCVDACPGGAIYLVPR
                     NYPKPQPKREDVVKALFALAESKVKTRALANGLGDDYPLARKALDFSLEVLAEDCVRE
                     GGYMLPQSVVTKEFLKDLRERYGDDPAFPVKAVERLLDLL"
BASE COUNT          496 a          670 c          600 g          480 t
ORIGIN      
        1 ccctcttttc ctacctcttt ctgatctcgc tcgccggaac gatgagtagc gggtatgcga
       61 tgaaactcct gaaaagcttg ctgaaagaac gggtgtccct tatcggcatc agcgtggtcg
      121 gggcgttcgt cagcaatctt tcccagcttc aggtcgcttc ctggatcgtg tacggaccgg
      181 caatctgggt agcggctcct ctgatgctgg gcctcggaat gatcacctcg atcatcctcg
      241 gcgttttcgg tgagcgctac ctgcgccatg gatcatttcc cgccgcattc cttgccgact
      301 cctttgagcc ggttctgccg accgccgaag agcgggcccc tctggtcgaa cgaacggtgg
      361 ccctgatcat cgcgatcgga gcgatcctct tcgccaaagg tccgattccg cttttgggaa
      421 ttgccgtcct gatgtatgtg attcaaggga tatcgggtcg gaagatccgc ctcttcccgc
      481 cgctgatgct cctttcctcc ctaatcgtcc tctccctctt tgagccgaac ggccaagtca
      541 tcgttaccgt cggaaccttg gcgttgacga aaggctcgat cgccttggcc ctgaccaaag
      601 gcctgaggct gatcgccctg ctctccgcca gccaaagcct ggtttcctcc aacccccgcc
      661 tcaaaggcag actcggctcg ctcctggtgc tggccctctc ctacttcgga atcctcactt
      721 catgcttccg gacggcgaaa ggctcccttc cgcaacggat cgacggagcg ctcttctgcg
      781 cagcggaagg aagcctgcag gtatctgcca acaatcagca agagcggagc tcttccgctt
      841 tgatcatcat cacggcgatc ctcgtggtcg gaatatcgat cgcaagcagg gtgtggggtt
      901 agttccggct atgcaaagat caagtatttc tctaaggtaa agacatgccc tccatcaaac
      961 ttatctgcac ggatatcgac ggaacgctcc tgcgacccga ccacacgatc agccctcgga
     1021 caaagagggc gattcaggaa gcccacaaga ggggaatcat cgtagcgttg gtcagcggcc
     1081 ggatcggaaa gagccttgtg aagattcagg aagagctcgg catcaccggc ccgctgggca
     1141 ccctcaacgg atcgcttgtc ttggacgaga agggccgatc gatcctggaa catccgctta
     1201 aaaaggacga gattcatcag atcctcgaat gggcgaaaca gaccgatctg aacgcgtttg
     1261 tctatacgaa caccaactgg tacgcctcaa cagagggata ttggacggaa tatgaactga
     1321 aaatcagtgg gttcaaagga atcgtccgct ccttccatga tcatgaagga cggctcggag
     1381 ccgatgaaaa gccgttcaaa gtcctctgca tggacaaaga cccggagtat ctttccaaga
     1441 tggaacgaca actgatccag cagatgggtt cccggttcac catcctccga tcggggccga
     1501 tctacctcga gatcttccac cggggagtgg acaaggggga cgctgtcaaa gccctcggct
     1561 cccattatgg aatcccccgg gaagcgatca tggcggtcgg agattactac aacgacgtgg
     1621 cgatgctcaa agccgcaggg tacagcgtgg cgatgggaaa cgctcccgat gacatcaggg
     1681 agatggccga cgttgtgact cgcccgaaca ccgaagacgg gcttgccctg gcgatcgaat
     1741 caatcctata acagatcaag caggcgctcg accgccttga cggggaaggc cggatcgtcc
     1801 ccatatcttt ctctcaggtc tttgaggaac tcttttgtta caacgctctg cggcaacata
     1861 tacccgccct cgcggacaca atcctccgcc agaacctcca aggagaaatc gagggccttt
     1921 cgggcaagag ggtaatcgtc ccccaaccca ttggccagcg cccgcgtctt caccttcgat
     1981 tccgcaagag caaagagggc tttgaccaca tcttcccgtt tgggttgcgg cttggggtag
     2041 ttcctgggaa ccagatagat cgctccgccg gggcatgcat caacgcagag gcgacacccg
     2101 tccagacatt ttgaagcatc gatctgaccg ctttccgtat cggtcgcccc ggtcgggcag
     2161 acaaagaggc acatacaatc cttcgtgcat aagcgtaaat tacgggaagc aaccatcatc
     2221 tgacctccat cttatgaatt tttatg
//
LOCUS       DAOPUZ010000205         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_51703_length_2238_cov_3.337609, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000205 DAOPUZ010000000
VERSION     DAOPUZ010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51703_length_2238_cov_3.337609"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKN79_04110"
     CDS             <1..845
                     /locus_tag="PKN79_04110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ94981.1"
                     /translation="IVHGHIGSSAAIYLWVAKRFGLTTVAHSHGAGNSRDLKGFMYSV
                     FAYPTRFVADYFLGCSMAAGISRYGKRVARSPRFRLALNAIDLAPLRYDETIRTNTRK
                     ELDLEGKVVVGHIGRFDPLKNHSFLLAVFKEFLALEPNAHLVLVGDGEERSRIEGAIE
                     RLSLRKHVSLLGFRSDIPRLLMAMDLFLFPSISEGLGISLIEAQGTGLVSLASDTVPS
                     EVKITDLVHFLSLKEPPKKWAKTLHSLIDEAPRNDRTEDLRSHGYDVITASKELEAWY
                     LTIG"
     gene            complement(835..1647)
                     /locus_tag="PKN79_04115"
     CDS             complement(835..1647)
                     /locus_tag="PKN79_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94982.1"
                     /translation="MRRYVLLLLIVFLAPMLLFADAGLENALPLLGESEYRRLLDGEI
                     LESNTIGGKKILPFFVPETTAYTKAVQAEEVVDGFSVAAVSYIPFPSSYDGLTTLERQ
                     LRLFNTMRSISTQEGIEYISYRAGNKPKLLIERSSYMEDGKNLNKLLPDPVATVFPLG
                     AQSYVYQRDTSFGGNRYLHTYTNSDREIFVSIENISPIKVLGLFTAVPKGQLNINMAT
                     YQLEDGLLLMALTTIEGRKPEVKVLTISVDLPSAFMRRIRALQGWFVEQLNR"
     gene            complement(1658..>2238)
                     /locus_tag="PKN79_04120"
     CDS             complement(1658..>2238)
                     /locus_tag="PKN79_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HNZ94983.1"
                     /translation="ERMMKRLFDFVASLIGIILLSPVMLILAILVKLSSPGPVIFRQK
                     RVTRDEKVFTMYKFRTMRSDIPEGAKARWTVEDDPRVTRIGALMRKTSLDELPQLFNV
                     LGGSMSLVGPRPERPELVEEFNTTIPGYRLRHRFKAGVSGWAQVNGWRGNTSLERRIE
                     FDLYYIRNFSLLFDLKIILFTFVRGFINENAY"
BASE COUNT          497 a          634 c          617 g          490 t
ORIGIN      
        1 cgatcgtcca cgggcatatc ggaagctccg ccgcgatcta tctgtgggtc gccaagcgct
       61 tcggtcttac gacggtcgct cacagccatg gggcaggtaa cagccgggac cttaaagggt
      121 ttatgtactc ggtctttgcc tatcccaccc gcttcgtcgc cgattatttc ctcggctgct
      181 cgatggccgc cggcatttcg cgctatggaa aaagggttgc ccgttccccg cgcttccggc
      241 tggcgctcaa tgcgatcgac ctcgctccgc ttaggtacga cgaaacgatc cgaacgaata
      301 cccgaaagga gcttgatctt gaagggaagg tcgtcgtcgg tcacatcggc cggttcgatc
      361 ccttaaagaa ccattcattt ctgcttgccg tgttcaagga atttctcgcc cttgagccga
      421 atgctcatct ggtcctcgtc ggcgatggag aggagcgatc tcggattgaa ggggcgatcg
      481 agcgtctttc gttgcggaag cacgtctctc ttctcggttt tcgaagcgac atccctcggc
      541 tgctcatggc gatggatctc ttcctcttcc cctccatcag tgaagggctc ggtatcagcc
      601 tcatcgaggc ccaagggacg ggactggtga gtctggcctc ggacaccgtt ccctccgaag
      661 tgaagatcac cgacctcgta cacttccttt ccctgaaaga gccgccgaag aagtgggcaa
      721 aaacccttca ttccctgatc gacgaagcac cacgcaatga ccggacggaa gacctgcgca
      781 gccatggcta cgacgtcatc accgcttcaa aagagttgga ggcgtggtac ctgactatcg
      841 gttgagctgt tcgacgaacc aaccttgaag cgccctgatg cggcgcatga acgccgaagg
      901 aagatcgacg ctgatcgtaa ggactttcac ctcgggcttg cgcccttcga tcgtggtgag
      961 ggccatcaac agcaggccat cctccagctg gtaggtggcc atgttgatat tcagctgacc
     1021 tttgggcacc gcggtgaaca gccccaggac ttttatcgga ctgatgttct cgatcgacac
     1081 gaagatctcc cggtcgctgt tggtataggt atgaagatac ctgtttccgc cgaaggaggt
     1141 gtcccgctga tagacgtagc tttgagcccc caatgggaag accgtcgcca ccgggtcagg
     1201 caggagcttg ttgagatttt tgccatcctc catgtaggaa gaacgctcga tcaatagctt
     1261 cggcttgttt cccgctctat aggagatata ctcgattccc tcctgagtcg aaatggagcg
     1321 catcgtattg aagagccgca gctgccgctc gagagtcgta agcccgtcat aggaagaggg
     1381 aaacggaata tagcttaccg ccgcgacaga aaacccgtcg acaacctcct cggcctgaac
     1441 cgcttttgta tatgcggtcg tttccggaac aaaaaaggga agaatcttct tgccgccgat
     1501 cgtattcgat tccagaatct cgccgtccag aaggcgccgg tattcactct ctcccaagag
     1561 cggcagggca ttttccagcc ctgcatcggc aaagagaagc atgggtgcaa gaaacacaat
     1621 caacaagagg aggacatatc tgcgcatacg aatttcctta atacgcgttt tcgttgatga
     1681 aaccgcgaac aaacgtaaag agtatgattt tcaggtcaaa cagcagactg aagtttcgaa
     1741 tgtaatagag atcgaattcg atccggcgtt ccagcgaggt gttgcctctc cacccgttga
     1801 cttgcgccca tcccgagaca cccgctttga agcggtggcg aaggcggtaa ccgggaatcg
     1861 tggtattgaa ctcttcgacc agctccggcc gctccggccg gggtccgaca aggctcatcg
     1921 aaccgccgag gacattgaag agctggggca gctcatccag actggtcttg cgcatcagag
     1981 ctccgatccg ggtgactcga ggatcgtctt ccaccgtcca gcgggccttc gcgccctcgg
     2041 ggatgtcgct gcgcatcgtt ctgaacttgt acatggtgaa gaccttctca tcccgggtga
     2101 cccgtttctg cctgaagatg accggccccg gggagctgag cttcaccaag attgcaagaa
     2161 tgagcataac cggtgaaaga agaatgatgc cgataaggga agcaacgaag tcgaagaggc
     2221 gcttcatcat ccgctcga
//
LOCUS       DAOPUZ010000206         2227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_52105_length_2227_cov_2.400552, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000206 DAOPUZ010000000
VERSION     DAOPUZ010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52105_length_2227_cov_2.400552"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PKN79_04125"
     CDS             complement(<1..1129)
                     /locus_tag="PKN79_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNZ94984.1"
                     /translation="MIDAGVVQSAYNAPQAALLLTWLLKHEPNPTRERIKEVLSGIFI
                     RDAGYEHYYLAVQLARELIEFGKYKTPISPTFRDHLTYVGKPKGKVDGPTLVAGEAAF
                     VEDRLQAGYYSMAILRSPHAMAYITEIDTAKAEALEGVVAVITHKNVPDVYYMQAGQG
                     NPEPSPYDRKLMNRKVRHVGDRVAAVVAETIEIAREALKLIEVTYEVLKPVFTVEEAM
                     AEGASLVHNSVVEYVVGAPEGLDEYNKGADPRDGKVIYQFPLHGDIRRNIASAAHGEI
                     GDVEKGFKEADLVIERTYQTNQIQCTPLEPHVCYAKIDGGRLVLHASTQVPYHVRRIV
                     ARVCEIPEHKVRVIKERVGGGYGSKQDILVEDLVGYATWLTG"
     gene            complement(1147..1395)
                     /locus_tag="PKN79_04130"
     CDS             complement(1147..1395)
                     /locus_tag="PKN79_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94985.1"
                     /translation="MTIQFTVNGKRHRVDVDGSETLRAMLVRLGYTNVRDSDDGDGFA
                     GSDTIIFNDLPVYANLMNAVQADGGEILTADSMERAGT"
     gene            complement(1392..>2227)
                     /locus_tag="PKN79_04135"
     CDS             complement(1392..>2227)
                     /locus_tag="PKN79_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNZ94986.1"
                     /translation="KGGVMMKKFLVAQSKEEALSLKRLHAKSVFYAGGTEINRLESAV
                     QAEVAIDLSKLGLNRIEEKKDEIIIGSMATLQTIATSSEVPAWLKEAALFCGSFTRRN
                     MATIGGNFALMLGRSYVGPVLLASRAILLIATINEKGKYAEDEVPVREFHEHISSYQG
                     ALILGFKIRKSDRFVGTLRFAVSSQNRASVTVGFGAEKDLSKVRVYAAVYTVGVLRLK
                     ETESRIEKGEITTEEEIAKSVASEVTVKSDYTGSGEYKRYIAGEGVAMLYGEFLKGGR
                     R"
BASE COUNT          396 a          717 c          600 g          514 t
ORIGIN      
        1 tgccggtcag ccaggtcgca tacccgacga gatcctcgac gaggatgtcc tgcttggaac
       61 catacccgcc gccgacccgt tccttgatca cccgcacctt gtgttcggga atctcgcaca
      121 cccgggcgac aatccgccgg acgtggtagg gaacctgcgt tgaagcgtga agaaccagcc
      181 tgcctccatc gatcttggcg tagcagacgt gcggctccaa cggcgtgcat tggatctggt
      241 tggtctggta ggtgcgctcg atcacgagat ccgcttcctt gaaccccttc tcgacatccc
      301 cgatctcccc gtgggcggcg ctggcgatgt tccgccggat gtccccgtga agcgggaact
      361 gatagatcac cttgccatcc cggggatcgg cgcccttgtt gtattcatcg agcccctcgg
      421 gagcgccgac gacgtattcg acgaccgagt tgtgcaccag actcgctccc tcggccatcg
      481 cctcctcgac ggtgaacacc ggcttgagca cttcgtacgt gacctcgatc agcttcagcg
      541 cttcgcgggc gatttcaatg gtttccgcaa cgaccgcggc gacccggtcg ccgacgtggc
      601 gaaccttcct gttcatcagc ttgcggtcat acgggctggg ttcggggttc ccctggccgg
      661 cctgcatata gtacacatcc ggcacgttct tgtgggtgat gaccgccacc accccctcca
      721 gcgcctccgc tttggcggtg tcgatctcgg tgatgtacgc catcgcatgg gggcttcgaa
      781 gaatcgccat cgaatagtag cccgcctgaa gccggtcttc cacgaacgcc gcctctccgg
      841 cgacgagggt cggtccgtcg actttccctt tcggctttcc gacatacgtg agatgatcgc
      901 ggaacgtggg gctgatcggg gtcttgtact tcccgaactc gatcagctcg cgggccagct
      961 gaaccgcaag atagtaatgc tcgtatcccg catcccggat gaagatgccg ctcaagacct
     1021 ctttgatccg ctcgcgggtg ggattgggtt catgcttcag aagccaggtc agcagcaacg
     1081 ccgcctgagg ggcgttatag gcgctctgga cgactcccgc atcgatcatc gcccgttgga
     1141 cgggattcag gttccggctc gttccatcga atcggcggtg aggatctctc ctccgtccgc
     1201 ctggaccgca ttcatcaggt ttgcgtacac gggcagatcg ttgaagatga tcgtatcgct
     1261 gccggcaaat ccgtccccgt catcgctgtc gcggacgttg gtatatccga ggcgaaccag
     1321 catcgctcgc aatgtttcag agccatcaac gtcaaccctg tgtctttttc cgtttaccgt
     1381 gaattgaatg gtcatcgtcg gcctcctttg agaaattcac catagagcat cgcgactcct
     1441 tcaccggcga tgtagcgctt gtactcgccg ctgccggtgt agtcgctctt caccgtgacc
     1501 tctgaggcga ccgacttggc gatctcctct tcggtggtga tttcaccttt ctcgattctg
     1561 ctttcggtct ccttgagccg cagcactccg acggtataga ccgccgcgta cacgcgaacc
     1621 ttcgaaagat ccttctccgc tccgaagccc accgtgactg aagcccggtt ctggctgctc
     1681 accgcaaagc ggagcgtccc gacgaaccgg tcgcttttcc tgatcttaaa tcccaggatc
     1741 agggctccct gataggagga gatgtgctca tgaaactccc gaaccggcac ttcatcctcg
     1801 gcgtatttgc ccttttcatt aattgtagcg atcaggagga tcgccctgct tgccagcaga
     1861 accggtccga catacgagcg accgagcatc aacgcgaagt tccccccgat tgtcgccatg
     1921 ttccgccggg tgaaggatcc gcagaagagc gccgcttcct tcaaccaggc gggaacctcc
     1981 gaactggtcg cgatcgtctg cagggtcgcc atcgaaccga tgatgatctc atcttttttc
     2041 tcttcaatcc tgttcaatcc gagcttggaa agatcgatcg ccacctccgc ctgcaccgcg
     2101 gattccaggc ggttgatctc cgtccctccg gcgtagaaca cactctttgc atgtaagcgt
     2161 ttcaggctca acgcttcctc tttactttgt gctacgagga atttcttcat cataacgcct
     2221 cctttcg
//
LOCUS       DAOPUZ010000207         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_52388_length_2219_cov_2.708410, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000207 DAOPUZ010000000
VERSION     DAOPUZ010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52388_length_2219_cov_2.708410"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2219)
                     /gene="ppdK"
                     /locus_tag="PKN79_04140"
     CDS             complement(<1..>2219)
                     /gene="ppdK"
                     /locus_tag="PKN79_04140"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNZ94987.1"
                     /translation="GAKLGDETNPLLVSVRSGAASSMPGMMDTILNLGLNPKSVQGLI
                     AKTGNERFAWDSYRRFMQMFGDVVMGVPHALFEGALQDVKDERGVKLDTDLDAADLQE
                     VIKRYQKIYHDTVGTDFPYDPVEQLFLATNAVFKSWNNERAIRYRQMNDIRGLLGTAV
                     NIQSMVFGNMGETSGTGVAFTRDPSTGENHFYGEYLMNAQGEDVVAGIRTPEPIETLE
                     RVNPEAYRQLVDIRHLLEQHYKDMQDLEFTIQEGTLYILQTRSGKRTVFSWLRSQVEM
                     VEEGLITKEEAVSRVPAGEFGKLFAPVLDSKYISDHGVDVITKGLNASPGGASGKAYF
                     TAAKAEEMAAKGEDVILVRNETSPEDIGGMAVAKGIVTARGGMTSHAAVVARGMGCPC
                     VSGAHEIVINEEKKFLTVGGVTIKEGEYIALDGFTGEVYAVEVPVKASEIVQVLNSQM
                     KEEESQLFGFYKKFMSYVQEIKRMAVYTNADTPQDTRMAVLFGAEGIGLCRTEHMFFG
                     EGRILPMRKMILAGNTIEREKALDQLLPMQRSDFEGIYEALAGRPATIRLLDPPLHEF
                     LPNDHTSRHELALQMGIPVEDLSQKISSMHEFNPMLGFRGCRLGIIYPEILRMQVRAI
                     IEAAINVKRKGIDVLPEIMVPLVGTYKEFVFTKKIILKVIEAIFAEQKMEIEYKIGTM
                     IEVPRAALTADEIAPHAEFFSFGTNDLTQMACGFSRDDSASFLEPYVNDPDKGFYDY"
BASE COUNT          419 a          657 c          632 g          511 t
ORIGIN      
        1 gtagtcgtag aagcccttgt cgggatcgtt gacgtacggc tcgaggaagg aggccgaatc
       61 atcccggctg aacccgcatg ccatctgggt gaggtcgttg gtgccgaagc tgaagaactc
      121 ggcgtgcggt gcgatctcat cggcggtcaa cgcggccctg gggacctcga tcatcgtacc
      181 gatcttgtac tcgatctcca tcttctgctc ggcgaagatc gcttcgatga ccttgaggat
      241 gatcttcttg gtgaagacga actccttgta ggtgcccacc agcggaacca tgatctccgg
      301 cagcacatcg attcccttgc gcttcacgtt gatcgcggcc tcgatgatcg cccgaacctg
      361 catgcgaaga atctcgggat agatgattcc aagccggcag ccgcggaagc cgagcatcgg
      421 gttgaactcg tgcatcgagc tgatcttctg ggaaagatcc tccacgggaa tgcccatctg
      481 cagggccagc tcatggcggc tggtatggtc gttgggaagg aattcatgga gcggcggatc
      541 gaggagtcga atcgtcgcag gtcttccggc aagggcttcg tagattccct cgaagtcgct
      601 gcgctgcatc gggagcagct gatccagcgc cttttcccgt tcgatcgtgt ttcctgccaa
      661 aatcatcttc cgcatcggaa ggatccggcc ttccccgaag aacatatgct cggttcggca
      721 gagcccgatt ccctcggcgc cgaagagcac cgccatccgg gtgtcctgcg gggtgtccgc
      781 gttggtgtag accgccattc gcttgatctc ctggacatag gacatgaact tcttatagaa
      841 cccgaaaagc tgggattcct cttccttcat ctggctattt aaaacctgga cgatctcgga
      901 ggccttcacg ggaacctcga ccgcatacac ttctccggtg aatccgtcaa gggcgatata
      961 ctccccttcc ttgatcgtaa caccgccgac ggtgaggaat ttcttctctt cgttgatcac
     1021 gatttcatga gcgccgctga cgcaggggca acccattccc cgggccacga ccgctgcgtg
     1081 gctggtcatg ccgcctcgag cggtgacgat tcccttggcg accgccattc caccgatatc
     1141 ttccgggctg gtctcgtttc gcaccagaat gacgtcttca cccttggctg ccatctcttc
     1201 cgctttcgcc gcggtgaagt acgctttgcc gcttgcgccg ccgggggagg cgttgaggcc
     1261 cttggtgatc acatcgacgc catggtcgga gatatacttc gagtcgagaa ccggagcaaa
     1321 gagcttgccg aattcaccgg cgggaacgcg gctgaccgcc tcctccttgg tgataagccc
     1381 ctcttcgacc atctcgacct ggctgcggag ccagctgaag acggttcgct ttccgcttcg
     1441 ggtctggagg atatagagcg ttccctcctg aatcgtgaat tcaagatcct gcatatcctt
     1501 gtagtgctgc tccaggaggt ggcggatatc aaccagctgc ctgtatgctt cggggttcac
     1561 cctctcgagc gtttcaatcg gctcgggggt tcggattccc gcaacgacgt cctcgccttg
     1621 ggcgttcatc aggtactcgc cgtagaagtg gttctcgccg gtggaggggt cgcgggtgaa
     1681 ggccacgccg gttccgctcg tctcgcccat gtttccgaag accatcgatt gaatgttgac
     1741 cgcagtcccc aggaggccgc ggatatcatt catctggcga tacctgatcg cccgctcgtt
     1801 gttccaggac ttgaacaccg cattggtagc gaggaaaagc tgttcgacgg ggtcgtaggg
     1861 gaaatccgtt ccaacggtgt cgtggtagat cttctgatag cgcttgatga cttcctgcag
     1921 atcggcggca tccagatcgg tatccagttt cacgccccgc tcatctttca catcttgaag
     1981 ggcaccttca aagagagcgt ggggaacccc catgacgaca tcgccgaaca tctgcatgaa
     2041 gcggcgatag ctgtcccaag cgaagcgctc gtttccggtc tttgcgataa gaccctggac
     2101 actcttcgga ttgagtccga ggttcaaaat cgtgtccatc attcccggca ttgaagaagc
     2161 ggctccgctt cgaaccgaga ccaacagcgg attggtttca tctccgagct ttgcgccca
//
LOCUS       DAOPUZ010000208         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_52520_length_2215_cov_3.536574, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000208 DAOPUZ010000000
VERSION     DAOPUZ010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52520_length_2215_cov_3.536574"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /gene="aspA"
                     /locus_tag="PKN79_04145"
     CDS             <1..147
                     /gene="aspA"
                     /locus_tag="PKN79_04145"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF021900.2"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNZ94988.1"
                     /translation="VPRLGYELCAQIAKKAQKTGGSVYEIVLEEGHLSKEELDQLLSP
                     EAML"
     gene            complement(202..1122)
                     /locus_tag="PKN79_04150"
     CDS             complement(202..1122)
                     /locus_tag="PKN79_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94989.1"
                     /translation="MVRFVPDEGLWLKGNLHAHSTLSDGNLTPDRVIAAYEERGYDFF
                     ALTDHDYYARYAPREKMILIPGFELAGYMGLKRVHLNVFEKGPVSRFAQGQRFSIQNE
                     EETRAFIEAYKDEYLFMLNHPDWSLLEYRDIEAYDQFFALEVMNWGTEYYDGIGEGSY
                     FWETGLRYGRRWGAIATDDNHNGYTDEPGWPFGNTGNDSFGAWIMVKAKVRTRQAIIA
                     ALEAGHYYASMGPEIHSFTVEDGWAEVHCSPVSRIIFSGENRNLRRRLGENLTHFRAP
                     LRGNKEHVKVRIIDEKGKTAYSNPIYLKDV"
     gene            complement(1607..>2215)
                     /locus_tag="PKN79_04155"
     CDS             complement(1607..>2215)
                     /locus_tag="PKN79_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HNZ94990.1"
                     /translation="QDRRGAGVIFDLPSYRDEAVYQVLLHLFYLVDVQSGLFYHAWSF
                     ERMDNFAKALWGRGNGWLTIFIPDFLEVLDVEPAVRKFIVTRFVRQLESLAEHQDESG
                     LWHTLINDPDSYLEASGSAGFCYGFYKAIEAGYLDKQYLDVANKALKAIVERIDEAGC
                     LRDVSYGTNVGTTLDHYRKIPLRSMQYGQGMALLALAAKTNG"
BASE COUNT          502 a          610 c          552 g          551 t
ORIGIN      
        1 gttccgcgcc tcggctatga gctgtgcgcc caaatcgcga agaaagccca gaagaccggc
       61 ggatcggtgt atgagatcgt gctggaggaa ggccatctca gcaaggaaga gctggatcag
      121 ctcctctccc ccgaagcgat gctgtagatc gaaccatccc ggtggaggct cttcaccggg
      181 atttcttttg ctttccgctc gttacacatc cttcagatag atcgggttgc tgtacgctgt
      241 cttgcccttt tcatcgatta tccgaacctt gacgtgttcc ttgtttcccc tcagcggagc
      301 gcgaaagtgc gtgaggtttt ctccaagtct ccgtctcaaa ttgcggttct caccgctgaa
      361 gatgatcctg ctcaccggtg aacaatgcac ctccgcccac ccgtcttcaa cggtgaagga
      421 atggatttcc ggccccatcg aggcgtaata atgccccgcc tcaagggcgg cgatgatcgc
      481 ttggcgggtg cgcactttcg ccttgaccat gatccaggcg ccgaaggagt cgttaccggt
      541 attgccgaac ggccaacctg gttcatccgt gtatccgttg tggttgtcgt cggttgcgat
      601 cgcaccccac cttcgtccat aacgaagacc tgtttcccaa aaatacgacc cctcgccgat
      661 cccgtcgtaa tattcggtgc cccagttcat cacctcaagg gcaaagaact gatcgtacgc
      721 ctcgatgtcg cgatactcca gaagcgacca gtcgggatga ttgagcatga acagatactc
      781 atccttgtac gcttcgatga aggcgcgggt ttcttcctcg ttctgtatcg agaagcgttg
      841 gccttgggcg aatcgggaga ccggcccctt ctcgaacacg ttgaggtgca cccgtttcaa
      901 gcccatgtat ccggcaagct cgaagccggg gatgaggatc atcttttcac gcggggcata
      961 ccgggcatag taatcgtggt cggtgagcgc gaagaaatca tatccccgct cttcatacgc
     1021 cgcaatcacc cgatccggcg tcaaattgcc gtcactgagg gtggaatggg cgtggagatt
     1081 gcccttgagc caaagacctt cgtccggaac aaaccgtacc atgcccattt cttccgcctg
     1141 ttcatctgat ttcttgttgg catcatacca tgtcgtccaa tcatcgaaaa cttctcacaa
     1201 gcggttattt ttggaatatg ctctccatat tgtatcggtc ggagacggcg tctttgtttt
     1261 atcaagataa cggccttgtc ggaaacaaat tttgaaggta acgccggtct cctgttgtgg
     1321 aggccggcat aaagaagtgt tatttgaaat acatggcgat tggcagtacg tgcaggagtg
     1381 actttgagct cctcgaggaa cactaaaaag tcgtgcaagt ttgacggaca ggtaaccacg
     1441 gcatcaatgt tgatccggct tactccgtca ctgaccgctt taaacatgga tattgagggt
     1501 gaagttgtag agctggaggt atcggtcgat tttttgggag atccatctct tttgtcgtca
     1561 tacttcgtta caaatcctta aaatatatcg aactacttgc gctatttcac ccgttcgtct
     1621 ttgccgccag agccaagagg gccatccctt ggccatactg catcgatctg agtggaatct
     1681 ttcgatagtg gtcgagggtc gttcccacat tcgttccata ggagacatcc ctgaggcacc
     1741 ccgcttcgtc aatacgctcg acaatcgctt tcagggcttt gtttgcaaca tccagatatt
     1801 gtttatcaag gtatcccgct tcaatcgcct tatagaatcc ataacagaac cccgctgatc
     1861 ccgaggcttc aagataggag tcggggtcat tgatcaaggt gtgccacaac ccgctttcat
     1921 cctgatgctc agcgaggctt tcaagctgcc tcacaaaacg ggtaacgatg aatttcctta
     1981 ccgccggctc cacgtccaga acttccagaa aatcgggaat gaagatcgtc aaccacccgt
     2041 ttccccgtcc ccacaacgcc ttggcgaagt tgtccatccg ctcaaacgac caggcatgat
     2101 agaaaaggcc actttgcaca tcgacaagat agaagaggtg caggaggacc tgatacaccg
     2161 cttcatcgcg gtaggaagga aggtcgaaaa tcactccggc acctcttcta tcttg
//
LOCUS       DAOPUZ010000209         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_52841_length_2207_cov_3.082249, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000209 DAOPUZ010000000
VERSION     DAOPUZ010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52841_length_2207_cov_3.082249"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PKN79_04160"
     CDS             <1..861
                     /locus_tag="PKN79_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94991.1"
                     /translation="GVAWNPIDGAGNQLSFVYLDCLSNAEMLVAKKGVFKDANGDGKY
                     DFEEIYQGLKGKKVYIEVGTTPGGWFKTLLNLINEGRPVNQQLWISSETASYIAGYKA
                     PNTNAANRVEVVNTLNSNLPAGMASAAGGMDIVVGFSPATSTIIRTNPNTELVATTVS
                     HFPPSASFPSTWVASNKWMEENPEVAQAFINALYEASVWRAENIDEAMRIGERLSQKP
                     EGTFDPFNLVAPSKKDLQAWFANKDGAGYHYLKGLYNVAKGNVPAGNKVKSFEEAFND
                     TFLLKAIATL"
     gene            919..2199
                     /locus_tag="PKN79_04165"
     CDS             919..2199
                     /locus_tag="PKN79_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94992.1"
                     /translation="MEHAVSCEVTISGIKESAVDSFILYNEGLIQSGLSAEAYAEGPL
                     GWFDPNTSMQSLQAIQSLAARVQARFDLLIVIGIGGSNRGAVAAVGALSPTKTELFFA
                     GDTLSATQLAKAIRLVQERNAALCVIAKDFNTIEPGITFRMLRKELERKYGSSSHERI
                     IAIGSGGEGQLHHLAQRHQWEFLAFPADINGRYSVLSTVGLFPMAVAGVDIESLIAGG
                     IAEHQRLTTLKPLMNPAVRYAAIRNLLSKQGSIIEALVIFEPALEGFARWWVQLFGES
                     EGKKEGALFPTYFSYSEDLHAIGQYVQEGPRIIVETFLNYTYKSSPLVIEPSCEIQDR
                     FDYLDDRPFDTLNEVVYQAALNAHASDHRPVIELSSSQSISAYSLGQSFYFFLFSVYL
                     SSLLLGVNPFTQDGVEGYKKNMYHQLGKGKVWPD"
BASE COUNT          524 a          583 c          606 g          494 t
ORIGIN      
        1 ggtgtcgcgt ggaacccgat cgacggggcc ggaaaccagc tttccttcgt atatcttgat
       61 tgtctctcga acgccgagat gcttgtcgcc aagaaagggg tcttcaagga tgccaacggt
      121 gacggaaagt atgactttga agagatctat caaggcctga aggggaagaa ggtatatatc
      181 gaagtcggta caacccccgg cggctggttt aaaactctct tgaatctgat caacgaaggc
      241 cgacccgtca accagcagct gtggatcagc agcgaaaccg cttcctatat cgcaggatac
      301 aaggcaccga acaccaacgc cgccaaccgg gtcgaagtgg tgaacaccct gaactccaac
      361 cttccggccg gcatggcaag cgccgccggc ggcatggaca tcgtcgtcgg gttctctcct
      421 gcgaccagca cgatcattcg gaccaacccc aatactgaat tggttgcaac gaccgtttcc
      481 cacttcccgc cgtcggcatc cttcccgtca acatgggttg cctccaacaa gtggatggaa
      541 gagaatcccg aagtagctca ggcgttcatc aatgcgttgt atgaagcgtc ggtctggagg
      601 gcggaaaaca tcgatgaggc gatgagaatc ggcgaacgat tgagtcagaa accggaaggc
      661 acgtttgatc cgttcaacct tgtggctccc tccaaaaagg atctccaagc ctggtttgcc
      721 aataaggatg gcgccggata tcattacctg aaagggttgt acaacgtggc taaaggaaac
      781 gttcccgcag gaaacaaggt gaagagcttc gaagaggcgt tcaacgatac gttcctcctc
      841 aaggcgatcg ccaccctgta agtggataaa ccatctgcat tggcagggag atgccttgcc
      901 aatgcggatc tcttgttgat ggagcatgcc gtgtcatgtg aagtaacgat ttccggaatc
      961 aaagaaagcg ccgtcgactc cttcattctg tacaacgaag ggcttattca aagcggtctt
     1021 tccgccgagg cgtatgccga gggaccgttg ggatggttcg atcccaacac ctcaatgcaa
     1081 tcgcttcagg cgatacaaag ccttgccgcc cgggtgcagg cacggttcga tctgctcatc
     1141 gtcattggaa tcggcggttc aaaccggggt gccgtggcgg cggttggcgc actttccccg
     1201 acgaaaacgg aactgttttt tgccggggat acgctctcgg cgacacagct ggcaaaagcg
     1261 atccgcttgg ttcaggagcg aaacgcagcc ctgtgcgtca ttgccaagga tttcaacacg
     1321 atcgagccgg gaatcacatt tcggatgctt cgcaaagagc tcgaacggaa atacggatcc
     1381 tcttcccatg aacgaatcat agcgatcggt tcgggcggcg aaggtcagct ccaccatctt
     1441 gctcagcgtc atcagtggga gtttttggcg tttccagccg atatcaatgg tcgttattcc
     1501 gtcctgagca cggtaggcct gttccccatg gcggttgcag gggtggatat cgaaagcctg
     1561 atcgccggcg ggatcgccga gcatcaacgg cttacgacgc tcaaaccgct gatgaatccg
     1621 gcagttcgct atgcggcgat ccggaatctg ctgagtaaac agggaagcat cattgaagcg
     1681 ctggtgatct ttgagccggc cttggagggg ttcgcccgct ggtgggttca gcttttcggc
     1741 gaaagcgaag gaaaaaagga gggagctctg tttccgacgt acttctccta ttcggaagat
     1801 cttcatgcga tcggtcaata tgttcaggaa ggaccgcgca tcatcgttga aacgtttctc
     1861 aactatacct ataaatcatc gccgctggtg atcgagcctt cttgcgagat tcaagaccga
     1921 ttcgactacc tcgatgatcg tccgttcgat acgctcaatg aagttgtcta ccaagccgcc
     1981 ttgaacgctc acgcctccga ccatcgaccg gtgatcgagc tttcgagctc gcaatcgatt
     2041 tccgcctatt cgcttggaca gtcattctat tttttcctct tcagcgtata tctctcttcg
     2101 ctgctcctcg gagtgaatcc cttcacacag gatggggtgg aagggtataa gaagaacatg
     2161 tatcatcagt tgggaaaggg aaaggtgtgg ccagactaaa agatatc
//
LOCUS       DAOPUZ010000210         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_52944_length_2204_cov_3.390879, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000210 DAOPUZ010000000
VERSION     DAOPUZ010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52944_length_2204_cov_3.390879"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /gene="proB"
                     /locus_tag="PKN79_04170"
     CDS             <1..1074
                     /gene="proB"
                     /locus_tag="PKN79_04170"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNZ94993.1"
                     /translation="NLLSTKAGIDEAYIDTIAAQIAALMEKGYQVLLVSSGAIGMGAK
                     ELRLKGPVKQTALRQACASIGNPLLMSSYRRAFKAHNLICSQILLTRLDVNNRRTYVN
                     LRNSVSTLLELGVVPIFNENDVVSIDSLGSAFGDNDRMSAFIASKIDAELLIILTDID
                     GLYDADPKKDPAANLLSEIDTLDSTILSYASGAGSTYATGGMKTKLLAAKIASFAGCG
                     TIIASGYESDIMVRILDGEQLGTYIHPAKALSQRQRWILNNSFQGAIVIDEGAKNALL
                     RKKSLLPKGVVKVDGTFAEGAIIEVRTPDGAPFARAVSSFSSTDLRRVAGHDSSEFAA
                     ILGNSHKGVIFRPEDMVFLEHDD"
     gene            1064..1963
                     /locus_tag="PKN79_04175"
     CDS             1064..1963
                     /locus_tag="PKN79_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94994.1"
                     /translation="MTTNYRIYSARDMHTSYGVDQLAIALIDSGSAEELIESIELYQQ
                     NMPRVPLFVITQDGRFDFPRLQELYRRISLIVFSGNTYIGEKVNAVASVCNTNYFLLL
                     RTDAVVVTFEYDRLLSLMSRSNHPVVASAIIANAYREIVPCLRTPTPFEKILDPGSDF
                     PSMDPDKPDHTLYPVMGLGFYDRALFQRLRGYDELIHSDFFQLMDLGLRSYLFGYRIT
                     LSPDLLIQFPNRESIIEDRSERTGFERCYTKNLSIRRVKGKNKVRKPPANYDRSSYRG
                     EVRERHQWLQKIDYDGLVTAWQK"
     gene            1951..>2204
                     /locus_tag="PKN79_04180"
     CDS             1951..>2204
                     /locus_tag="PKN79_04180"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNZ94995.1"
                     /translation="MAKIVRTIILLMCLIAVPLFSVDNPFALPLGTIVIDAGHGGHDP
                     GAIRSFEGSLIVERELTLDIALRLERLLNEAHPSLNVVMTR"
BASE COUNT          475 a          641 c          603 g          485 t
ORIGIN      
        1 aacctgctct ccacaaaggc ggggatcgac gaggcgtata tcgatacgat cgccgcgcaa
       61 atcgccgcat tgatggagaa ggggtatcag gtgctgcttg tctcctccgg agcgatcggg
      121 atgggggcga aagagcttcg cttgaaagga ccggtgaaac agaccgccct ccgtcaggcg
      181 tgcgcctcga tcgggaaccc attgctgatg agctcttacc ggcgagcatt caaagcccac
      241 aatctgatct gctcccagat cctccttacc cgcctggatg taaacaatcg gcgcacatac
      301 gtgaacctcc gcaacagcgt ttcgaccctt ctggaattgg gggtagttcc catcttcaat
      361 gagaatgacg tcgtttccat cgattcgctc ggctccgcgt tcggggacaa cgatcgaatg
      421 agcgcattca tcgcgagcaa gatcgacgcc gagctcttga tcatcctcac cgacatcgac
      481 ggcctctatg atgcggatcc caaaaaggat cccgcggcga acctgttgag cgagatcgac
      541 acccttgatt cgacgatcct ctcctatgct tccggtgccg gttcgacgta cgccaccggc
      601 gggatgaaaa ccaagctcct tgcggcgaag atcgcttcgt tcgccgggtg cgggacgatc
      661 atcgccagcg gatatgaatc cgatatcatg gtgcggattc tggatggtga acaattggga
      721 acgtacatcc acccggcgaa agcgctcagc caacgccagc gatggatcct gaacaactcc
      781 tttcaggggg cgatcgtgat cgacgaggga gcgaagaacg ctctgttgag aaagaaaagt
      841 ctgctgccca agggggtggt gaaggtggac ggcaccttcg ctgaaggggc gatcattgaa
      901 gtgcggacac ccgacggcgc tccctttgcc cgggcggtct cctccttttc ctccaccgac
      961 ttgagacggg tcgccggtca tgactcgtcg gagttcgccg ccattttggg caactcgcac
     1021 aaaggggtga tcttccgacc ggaagatatg gtattcttgg agcatgacga ctaactatcg
     1081 aatctattcc gcccgtgata tgcacacctc ctacggcgtc gaccagctgg cgatcgccct
     1141 gatcgacagc gggagcgctg aggaactcat agaatcgatt gaactctacc agcagaacat
     1201 gccaagagtt cccttgttcg tcatcactca ggacgggcgg tttgacttcc cccggcttca
     1261 ggaactctac cgccggatct cgttgatcgt gttttccggc aatacctaca tcggagagaa
     1321 ggtgaacgcc gtcgcttcgg tgtgcaacac caattatttt cttcttcttc gcaccgacgc
     1381 cgtcgtggta accttcgaat acgaccggct tttgagtctg atgagccgaa gcaaccatcc
     1441 ggtggtcgct tcggcaatca tcgcgaatgc gtaccgcgag atcgtcccct gtctgcgaac
     1501 accgaccccc tttgaaaaaa tcctcgatcc cggctccgac tttccctcaa tggatccgga
     1561 caaacccgat cataccctct atcccgtgat ggggctcggc ttttatgacc gcgccctctt
     1621 tcaacggctt cgcggctatg acgagctgat tcacagtgat ttctttcaac tgatggatct
     1681 gggcctgcgc agctatctgt tcggatatcg gataaccctc agccccgatt tgctgattca
     1741 atttcccaat cgggaatcga ttatcgaaga tcgaagcgag cgtaccggat ttgagcgctg
     1801 ttatacgaag aacctctcga tccggcgggt gaaaggcaag aacaaagtgc gaaaaccacc
     1861 ggccaactac gatcgctcct cctaccgggg ggaagtgagg gagcggcatc agtggttgca
     1921 gaagatcgac tatgacgggt tggttaccgc gtggcaaaaa tagttcgaac aatcattctg
     1981 ctgatgtgtc tcattgccgt accgctcttt tcggtggata atcccttcgc ccttccgctc
     2041 ggcacgatcg tcatcgacgc cggtcacggc gggcacgacc ccggagcgat ccgctctttt
     2101 gaaggttcgc tgatcgtcga acgggagctg acgctggata tcgccctccg tctggaacgg
     2161 ttgctgaatg aggcccatcc gtccctgaat gtggtgatga cccg
//
LOCUS       DAOPUZ010000211         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_53021_length_2202_cov_3.081043, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000211 DAOPUZ010000000
VERSION     DAOPUZ010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53021_length_2202_cov_3.081043"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..749)
                     /locus_tag="PKN79_04185"
     CDS             complement(117..749)
                     /locus_tag="PKN79_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein CapC"
                     /protein_id="HNZ94996.1"
                     /translation="MGLWDVHSHLLPEIDDGYLDRAGCIRMLEIYRECGIDHIVATPH
                     IYNPFVRTAVERIRSTFEWAKNEAEALGISLHLGSELYIADQGVLRAIPILDRFVLVE
                     FSLALPPPNLKEQLRKLIDDGFVPIIAHVERYVWLDPKHALVHKLKEMGCLLQGNVEA
                     VESGAALPYLEAELLDILATDNHGNERLPLSLVQTMEKWPFVKKRMEEIW"
     gene            complement(831..1355)
                     /locus_tag="PKN79_04190"
     CDS             complement(831..1355)
                     /locus_tag="PKN79_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNZ94997.1"
                     /translation="MVLIYTGNGKGKTSAAVGQAVRALGHDGKVALAQFIKQDPDLLD
                     SGEARILKELGVTWGSFGAGFTWIEGNEKENRILASKGWETVKRWALSGVYDLIVLDE
                     FTYPLTLGYVDVEEIVSFITEHKDDEKFPHLVITGRGAPETLVAIADMVSEVGEVKHH
                     LANSGLEARPMIEF"
     gene            complement(1359..1679)
                     /locus_tag="PKN79_04195"
     CDS             complement(1359..1679)
                     /locus_tag="PKN79_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94998.1"
                     /translation="MAKEIYWLVDRGEYRLAYHRFLGLYEDTGDWRWGYNAALLLQGI
                     GELENAVVLMDEVYSGYPSNLVFQALSRMRDALRDQQKAEMQICGECLEEFTLVTQMY
                     TEGR"
     gene            complement(1709..>2202)
                     /locus_tag="PKN79_04200"
     CDS             complement(1709..>2202)
                     /locus_tag="PKN79_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ94999.1"
                     /translation="LTLPSGYYSTLSEDVSYAATRTLERAIESTDHLSVTSSRAAEAV
                     IKSEITFMDLSERIIERSESEGSEEKSYFLVQSAAVALSIKVMEVSTGLILYTSNYAD
                     KVTKEYLVGTRMFDPTSNTYKIIRRFTFTRSPSFLPLFEEILRSFERTIARQLAPRRL
                     PNG"
BASE COUNT          510 a          634 c          524 g          534 t
ORIGIN      
        1 cggttatccc tgacggcttc aaacgaaatg gtctgttcgt ccctcacgga tttgaagttc
       61 gcaatagtca tatcaagcag catgcgctac ctcctcaatc tcacccttcg gtggtattac
      121 caaatttctt ccatccgttt ttttacgaat ggccactttt ccatcgtttg gaccaagctt
      181 aacggcaatc gctcattacc atggttgtca gttgccagta tatcaagaag ttccgcttca
      241 agatacggca aagcggcgcc actttcaacc gcctcgacat tgccttgcaa cagacatccc
      301 atttccttca acttatgaac cagcgcatgt ttgggatcca accacacgta tcgttcaaca
      361 tgggcgatga tcggaacaaa gccgtcgtca atcaacttcc taagctgctc cttcaagttg
      421 ggaggaggaa gagcgagcga aaattcgaca aggacgaatc ggtcaagaat cggaattgcc
      481 cgcaagacac cctgatcggc tatgtacagc tcgctgccga gatggagtga tattccaagc
      541 gcctcggctt catttttcgc ccactcgaat gtagagcgga tccgctcgac ggctgttctg
      601 acgaaggggt tgtagatatg gggtgttgca acgatatggt cgataccaca ctcgcgataa
      661 atctcaagca ttcggataca tcccgcccgg tccaagtatc cgtcatcgat ttccggcagc
      721 aaatggctgt gaacatccca caatcccata cagccctccc tttgcgccta ttataccctt
      781 aattactcac aaggggaagg aaaaagaggg ctcattacct tacaaggcca tcaaaattca
      841 atcattggcc gggcttcgag cccggaattt gccagatgat gcttcacttc accgacctcg
      901 ctgaccatgt cggcgatcgc gaccagtgtc tcgggcgcac cccgtccggt gatcacgagg
      961 tggggaaact tctcatcatc tttgtgctcg gtgataaagg aaacaatctc ttccacatcg
     1021 acatacccaa gggtcaacgg ataggtgaat tcatccaaaa cgatcagatc gtacaccccg
     1081 ctcaacgccc atcgttttac cgtttcccat cctttggagg cgaggattcg attttccttc
     1141 tcattgcctt caatccaggt aaagccggca ccgaacgatc cccacgttac tccaagctct
     1201 ttgagtatcc gtgcctctcc actgtccagc agatcgggat cctgcttgat gaactgggcc
     1261 aacgcaacct tcccatcgtg tccgagagcc cgaacggcct gtcccaccgc ggcagaggtt
     1321 tttcctttgc cattgccggt atagatcagc accatcggtc aacgtccttc cgtgtacatc
     1381 tgcgtgacga gtgtaaactc ctcaagacac tccccacaga tctgcatctc cgctttctgc
     1441 tgatcgcgga gggcatccct cattcgcgaa agggcttgga aaaccagatt ggacgggtag
     1501 cctgaataga cttcatccat caacacgacc gcattttcca gttcgccgat cccctggagc
     1561 agcaacgccg cattgtagcc ccacctccag tcgccggtat cttcatagag accgaggaac
     1621 cgatgatacg ccagccgata ttcacccctg tcgaccagcc agtaaatctc cttggccatc
     1681 gcgtccttcg gcctgttggg agccagattc acccattcgg taaccgtcga ggggcgagct
     1741 gtcgggcgat cgttctttca aatgagcgga ggatctcctc aaagagcggc aggaaggagg
     1801 ggcttcgggt gaaggtgaac cggcggatga tcttgtaggt attgctggtc ggatcaaaca
     1861 tccgggttcc gaccagatat tctttggtga ccttatctgc ataattggag gtgtacagga
     1921 tcaatccggt cgatacttcc ataaccttga tcgagagcgc gacggccgca ctctgcacaa
     1981 ggaagtagga tttttcttcg cttccttcac tttcactccg ttctatgatc cgctctgaga
     2041 gatccatgaa ggtgatctcg ctctttatca ccgcttcggc ggcccgactg gaggtgacgg
     2101 agagatgatc cgttgattca atcgcccgct ccaacgttcg ggttgcggca tatgaaacgt
     2161 cctcgctcaa ggtggaatag tatccgcttg ggagcgtcag ct
//
LOCUS       DAOPUZ010000212         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_53794_length_2181_cov_3.529633, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000212 DAOPUZ010000000
VERSION     DAOPUZ010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53794_length_2181_cov_3.529633"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKN79_04205"
     CDS             <1..417
                     /locus_tag="PKN79_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNZ95000.1"
                     /translation="LVKQALASPPAVRGPYADGEYYAEEGAFGSSGWKYFVSLLVRNG
                     NIVHANWSAVDKDGRDKRQVAASGEYGMIKVSSLKKEWHEQALEAEMYLLKTQDPKQI
                     ALKADGKTDAIAGATITVGVFFDLAEAALANGPRTY"
     gene            complement(480..563)
                     /locus_tag="PKN79_04210"
     tRNA            complement(480..563)
                     /locus_tag="PKN79_04210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(527..529),aa:Leu,seq:gag)
     gene            complement(633..>2181)
                     /gene="pepF"
                     /locus_tag="PKN79_04215"
     CDS             complement(633..>2181)
                     /gene="pepF"
                     /locus_tag="PKN79_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNZ95001.1"
                     /translation="LNWASDTSNPANVERSSISSTLMTELGAALAFFDPELLSIDEEV
                     MNGYLADEAFEPYKVFLMRSMRYKAHVLSEREERIMALQRESAQTPRNAFSDLTNIDF
                     DFGRIGDKPLTQSTFSSFLMNEDRALRKKAYKQFYSRYNRNRHTLFRLYEGQVKQDIF
                     RHTVRSYPSSREMSLFADDVPTGVYDRLIESVHAGLPVLHRYYELRKKILGVRNLAHW
                     DVYVPLVSGVKAHTPYEEAVKIIGEALKPLGSEYVDTLTRGLTIERWVDRYENKGKRS
                     GAFSSGSYTGYPYILMNYKEDVLRDLFTLAHEGGHSMHSYYSARNNPFHHWDYSIFEA
                     EVASTFNEQLVANYMIESTDDVQTKRYIIAKQLDDVVATLFRQTMFAEFEHKIHKKAE
                     ASIPLTLDLVRSTYGDLLRQYFGPGVTFIDESNLEGLRIPHFYSPFYVYKYATGLSAA
                     IALSKEVLGGGEEERARYLRFLSNGGRYFPIENLKQAGVDMSTGKPVDDAIAHFAQRL
                     ELFETLS"
BASE COUNT          463 a          584 c          652 g          482 t
ORIGIN      
        1 ttggtgaaac aggccctcgc ttcacctccg gccgtcaggg gtccgtatgc ggacggtgag
       61 tattacgctg aagaaggggc cttcgggagt tcggggtgga agtatttcgt ttcgttgctc
      121 gtccgaaacg gaaacatcgt ccatgcgaac tggagcgccg ttgataagga cggtcgggac
      181 aagcgtcagg ttgccgcttc cggcgagtat gggatgatca aggtgagttc tctcaagaaa
      241 gagtggcacg agcaagcgtt ggaagcggaa atgtacctgt tgaagactca ggatcccaag
      301 caaatcgcat taaaggcgga cggaaagacc gacgcgatcg ccggagccac aatcactgta
      361 ggcgttttct tcgacttggc tgaagcggcg ttggccaacg gaccgaggac atactgattt
      421 ttaaaatcag ctctttgcaa tatgaacgct ccgggggttc cggagcgttt tgccgtatct
      481 gcggtagact ggactcgaac cagcacaaga tctctcccac tagcacctca agctagcgtg
      541 tctaccaatt ccaccactac cgcacatgaa ttagttgaac agggcaacct tacaatgaag
      601 acgaattgaa ggtcaagccc tttttccgaa tcctaactga gcgtttcaaa aagctccagc
      661 cgttgggcga aatgggcgat tgcgtcatca accggcttgc cggtcgacat gtccacgcct
      721 gcttgtttga ggttttcgat cgggaaatac cgtccgccgt tggaaaggaa cctcagatag
      781 cgggcccgct cctcttcacc tcctccaagc acctctttgg agagcgctat cgccgcactg
      841 aggccggttg cgtacttgta gacgtagaag ggggagtaga agtgtggaat cctcaaacct
      901 tccagattgc tttcatcgat gaacgtaacc cccggtccga agtattggcg gagaagatcg
      961 ccatatgtgc tgcgaaccag atcgagcgtc aaggggatgg aagcttccgc cttcttgtgg
     1021 attttgtgct cgaactcggc gaacatcgtc tgcctgaaga gggtggcgac cacgtcatcc
     1081 agctgcttgg cgatgatgta gcgtttggtc tgaacatcat cggtggactc gatcatgtag
     1141 ttggcgacca gctgctcgtt gaacgtcgag gcgacctccg cctcgaagat cgagtaatcc
     1201 cagtggtgga aggggttgtt gcgcgccgag tagtaggagt gcattgagtg ccccccttca
     1261 tgggcgagcg tgaagagatc gcgcagcaca tcctccttgt agttcatcag gatatagggg
     1321 tacccggtgt aggagccgga ggagaacgcg ccgctgcgct tccccttgtt ctcgtagcgg
     1381 tcgacccacc gctcgatcgt gagaccgcgg gtcagggtgt cgacatactc gctgccaagc
     1441 ggcttgagcg cctcgccgat gatcttcacc gcctcctcgt acggcgtatg ggctttgact
     1501 ccgctgacca gggggacgta cacgtcccag tgggcgaggt tccgcactcc gaggatcttc
     1561 ttgcgtagct cgtaatagcg atgaaggacc ggcaggccag catggacgct ctcgatcaac
     1621 cggtcgtaga cgccggtcgg cacgtcatcg gcgaagagcg acatctcccg gctggatgga
     1681 taggaacgaa cggtgtgcct gaagatatcc tgtttcacct gaccctcgta gaggcggaaa
     1741 agggtgtggc ggttccggtt gtatctggaa tagaactgtt tgtacgcctt cttcctgagc
     1801 gcccgatcct cgttcatcag aaaggaggag aaggtggatt gggtcagcgg cttgtctccg
     1861 atccggccaa agtcgaaatc gatgttcgtc aggtcgctga acgcgtttct cggcgtctgc
     1921 gcactttccc gctgaagcgc catgatccgc tcttcccgtt cactgaggac gtgggccttg
     1981 tagcgcatgc tccgcatgag aaagaccttg tacggctcga acgcctcgtc cgccagatag
     2041 ccgttcatca cctcctcatc aatcgaaagc agttcgggat cgaagaacgc aagcgccgcc
     2101 ccgagttcgg tcatcaaggt gctggagatt gaggagcgtt cgacgttggc cggattcgag
     2161 gtgtcgctcg cccagttgag a
//
LOCUS       DAOPUZ010000213         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_53881_length_2179_cov_3.968927, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000213 DAOPUZ010000000
VERSION     DAOPUZ010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53881_length_2179_cov_3.968927"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1712
                     /locus_tag="PKN79_04220"
     CDS             <1..1712
                     /locus_tag="PKN79_04220"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNZ95002.1"
                     /translation="PKHGIFREIPIRYGNQRIGLKNLQASDPIIKDDVSSGWATFRLG
                     DANVTIKGLKEYVISYTLEIGDDRNRYGDFFYYNIIGPGWQEEIGLLTFTVEFPHPID
                     PQKVSLTGGRSGSTLQRGEYMISSDRMTITGSAQKMKPGEALTLYIDLEEGYFSEVKK
                     FIDLTIPLYIGAIILFLLLGFHSFALWKRYGRDEPFVPVVRFEPPTDLSPLEVGYLFD
                     GTVDMKDLSSMLFYWADLGYIQIEELGKKEYRIIKLKDLVSDKEHERRLFNAFFKRGD
                     GTSVDLKNLSKSTTFAKDIEKTKSLTRSYFTGERELKDQKAERKRTVPFLYGVLLVVF
                     HAWGTTYSYPGGVIFTLPLTFGIGLLVLTAVAIPKIFDSWMFARKGKALLKSGAFLLV
                     YLASFGIIYIMYREEVLLSSALSLLFSASALGIPIIFATFGAITAKRSSYGQKILEEI
                     VGYRGFIEKVEIDKLKVMIKDDPELFYHVLGYAVVLGLENTWAKKFDSIAMTQASWYI
                     GSGGIHPFFYASMANNLTTGAISKALYTQASSGSGRGPISPSFGGGGFSGGGFSGGGG
                     GAW"
     gene            complement(1766..2044)
                     /locus_tag="PKN79_04225"
     CDS             complement(1766..2044)
                     /locus_tag="PKN79_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNZ95003.1"
                     /translation="MAKKIYVGNMSYNTSEAELRELFAQFGTVLSANIIIDRETHRPK
                     GFGFVEMEDNNAAEAAISQLDGKEFGGRNLRVNEAIAKSRPAYGSHRN"
BASE COUNT          471 a          590 c          602 g          516 t
ORIGIN      
        1 ttcccaagca tggaatcttt cgggagattc ccatccggta cgggaatcag cggatcgggc
       61 tgaagaatct tcaggcgagc gatccgatca tcaaggacga cgtctcctcc ggttgggcga
      121 ccttccggct tggtgacgcc aatgttacca taaaaggtct caaagagtat gtgatctcct
      181 acaccttgga gatcggggat gaccggaatc gctacggtga cttcttctac tacaatatca
      241 tcggtcccgg atggcaggaa gagatcggcc tccttacctt tacggttgag ttcccacatc
      301 cgatcgatcc tcagaaggtc tccttgaccg gcggcaggtc cggctcgacc cttcagcggg
      361 gtgaatacat gatctcttcc gatcggatga cgatcaccgg aagcgctcag aagatgaaac
      421 ccggggaagc gctgaccctc tacatcgatc tggaggaggg gtacttcagc gaggtgaaga
      481 agtttatcga tctgacgatt ccgctctaca tcggtgcgat catcctcttc cttctgctgg
      541 gatttcattc ctttgcgctc tggaagcgct acggtcggga cgagcccttc gtaccggtgg
      601 ttcgctttga gcctccgacg gacctttcac cgttggaggt cggctacctc tttgacggaa
      661 cggtggacat gaaagacctt tccagcatgc tcttctactg ggcggatctg gggtacatcc
      721 agatcgagga actggggaaa aaagaatatc gcatcatcaa gctcaaagac cttgtttccg
      781 ataaagaaca tgaacgaagg ctcttcaatg catttttcaa acgcggcgac ggaacaagcg
      841 tcgatctgaa gaatctttcc aagtcaacca ccttcgccaa ggatatcgag aagacgaaga
      901 gtctgacccg ctcctacttc accggggagc gggagctcaa ggatcagaag gccgaacgca
      961 agcgaaccgt tccgttcctg tacggagtcc tgctggtcgt cttccatgcg tgggggacca
     1021 cgtacagcta tcccggcggc gtgatcttca cgcttccgtt gacgttcggg atcggcttgc
     1081 tggttctcac cgcggtcgcg attccgaaga tcttcgattc gtggatgttc gcccgcaaag
     1141 gaaaagcgtt gttgaagagc ggcgcatttt tgctggtata cctcgcttcc ttcggcatca
     1201 tatacatcat gtatcgcgag gaagtccttc tctcttccgc tctttcactg ctcttcagcg
     1261 caagcgccct cggaattccg atcatattcg ccaccttcgg agcaatcacc gccaagcgca
     1321 gcagctacgg tcagaagatt ctggaggaga ttgtcggcta tcgcgggttc atcgagaagg
     1381 tcgagatcga caagctgaag gtgatgatca aagacgatcc cgagctcttt taccatgtac
     1441 tcgggtatgc ggtcgtcctt ggtctggaga atacgtgggc gaagaagttc gactcaatcg
     1501 ccatgacaca ggcctcctgg tatatcggct cgggtggaat ccatccgttc ttctacgctt
     1561 cgatggcgaa caacctcacc accggtgcga tttcaaaggc gctctacacc caggcctctt
     1621 ccggttctgg ccggggcccg atcagcccct ccttcggcgg aggcgggttc agcgggggcg
     1681 gattcagcgg gggaggaggg ggagcctggt aatgaaaaaa cggagcctgc gggctccgtc
     1741 ctcgttgctg aatcaatggt attggttagt tgcggtgcga tccgtaagcc ggacggctct
     1801 tggcgatcgc ttcgttgacc cgcaggttgc ggccaccgaa ttccttgcca tcgagctggg
     1861 agattgcagc ttccgccgca ttgttgtctt ccatctcgac aaacccgaaa cccttggggc
     1921 ggtgcgtctc tcggtcaatg ataatgttcg cactgaggac agtgccgaac tgggcgaaca
     1981 gttcccgaag ttccgcttcg gaagtgttgt aactcatgtt accaacatag attttctttg
     2041 ccatgtacta aagcatcctt cacaggctgc gcctgtttga tcaatattgc ttaatgtggc
     2101 gcagttgtga ctcttacctg ccaatgggag tgattcagca acgccgatcg gacaactagg
     2161 gaaaatgaaa cgagctgca
//
LOCUS       DAOPUZ010000214         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_54143_length_2171_cov_3.495747, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000214 DAOPUZ010000000
VERSION     DAOPUZ010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54143_length_2171_cov_3.495747"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKN79_04230"
     CDS             <1..270
                     /locus_tag="PKN79_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95004.1"
                     /translation="TYGFASDLMSDVLTILKDVIIMITGLSNMQAVRTAEMSDIRNIL
                     LVRNKQPSQAMIEIAEELGISISYTPYSLFRVSGILYGAGLKPVY"
     gene            complement(267..>2171)
                     /locus_tag="PKN79_04235"
     CDS             complement(267..>2171)
                     /locus_tag="PKN79_04235"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M16"
                     /protein_id="HNZ95005.1"
                     /translation="FIAGFKGIDPEDGEKAQNVILSTLKKIVRDGLDPELIETSLKRT
                     RFSQLEIPGGIPTGLRALTRATRAWMVDRSPSETIELVPTLNELEKRIKRNPRYFEDW
                     IKDHLLDNPHRILVTVKPDEEHQRRQNEAIAKRALHAAKQPGRNGIKEIIEQNKRFAA
                     FEEERDSTENLEKIPFLRLEDLPKEIRPNDHALKIIAGRELWIRTIPTNKIVYADIAF
                     TIDDLTEEEMLLLNLYTRLVQTTGLGELPYETVAQRIKRLTGGFSFFVEVGTSLQGEA
                     VHLLIVRIKTLAEDFDEALGFIQELLVGTNVDDRKQLRFVLNNYKSEFSEVVTYGATN
                     FASSYATRTFSEAAWDAELIGGLSQWFHLTGLEADSLGGRLKALQTKLANRRRLVAHL
                     SCEEPLTEDSVKSFETFITRFEDRGPLDKEPRSYEKVGPTSNPVVDLFKLPSTVSYSA
                     WALRSAKRGSVLQSAQVLLSQILTTGPLWETIRGQGGAYGVSASADVAEETFILSTYR
                     DPRIAGTFTDLKRVLEEAATRVFSEKEIEDSTIFIVGRELRPLSPGQDALLGFRRILY
                     SITDEFRLMRRTQLLELSNEDLNEGAKALLEQAERDGSNLVVLASGELLGKEAKKLEI
                     LNVESRRLPL"
BASE COUNT          415 a          650 c          618 g          488 t
ORIGIN      
        1 acctatgggt ttgccagtga cttgatgagc gatgtgctca ccatcctgaa agacgttatc
       61 atcatgatca ccggcctgtc gaatatgcag gccgttcgaa ccgccgagat gagcgatatc
      121 cgcaacattc tccttgttcg aaacaagcag cccagccagg cgatgatcga gatcgccgaa
      181 gaactgggca tcagtatctc ctacaccccg tactccctct tccgagtgag cgggattctg
      241 tatggcgccg gtttgaaacc cgtctattaa aggggcaggc gccggctttc cacgttaaga
      301 atttccagct tcttcgcctc tttccccagc agttcgccgc ttgccagaac gaccagattg
      361 cttccgtccc gctccgcttg ttcaagcagc gctttcgctc cctcattgag gtcttcgttt
      421 gagagctcaa gaagctgggt tcgccgcatc aggcggaact cgtcggtgat cgagtagaga
      481 atcctgcgga accccagaag cgcatcctgg ccgggtgaaa gcggcctgag ctcccgtccg
      541 acgatgaaga tcgtactgtc ttcaatctct ttttcactga aaacacgggt cgcggcttcc
      601 tcaagcaccc tcttcaggtc ggtgaacgtt ccggcgatcc tcggatcccg ataggtggaa
      661 agaatgaacg tctcctcggc cacatcggca ctggcggaaa ctccgtacgc tccgccttgc
      721 ccccggatcg tttcccaaag gggccccgtg gtaagaatct gcgagaggag aacctgagcg
      781 gattggagca cgcttccccg cttggcggag cgaagcgccc aggcgctgta gctgaccgtc
      841 gacggcagct tgaagagatc gacgaccggg tttgaagtcg gcccgacctt ctcgtagctc
      901 ctcggctctt tgtcaagcgg tccccggtct tcaaagcggg tgatgaacgt ctcgaagctc
      961 tttaccgaat cttccgtcaa aggctcctcg cagcttagat gggcgaccag ccttcggcgg
     1021 ttcgccagct tcgtctgcag ggctttgagc cgcccgccga ggctgtcggc ttcaagcccg
     1081 gtaagatgga accactggga aagcccgccg atcagctcgg cgtcccacgc cgcctcgctg
     1141 aaggttcggg tggcgtagct cgaggcgaaa ttcgttgcgc cataggtgac gacctcgctg
     1201 aactcgctct tgtagttgtt gaggacgaag cgcagctgct tcctgtcatc cacattcgtt
     1261 ccaacaagga gctcctgaat gaagcccagc gcctcatcga aatcctccgc caaggtcttg
     1321 attcgaacga tcagaaggtg aaccgcctct ccttgaaggg aagttcccac ctcgacgaag
     1381 aaggagaagc cgccggtcaa ccgtttgatc cgctgcgcca ccgtctcata aggcagctcg
     1441 ccaaggccgg tggtctgaac cagacgggtg tagaggttga gaaggagcat ctcctcttca
     1501 gtcaaatcat caatcgtgaa ggcgatgtcg gcgtagacga tcttgttggt gggaatcgtg
     1561 cggatccaga gctccctgcc ggcaatgatc ttcagggcat ggtcgttcgg ccggatctct
     1621 ttcggcagat cctccaaccg gaggaagggg atcttctcca gattctccgt gctgtcccgc
     1681 tcctcctcga aggcggcaaa gcgcttgttc tgctcgatga tctcctttat cccgtttctg
     1741 cccggctgtt tagcggcgtg gagcgcccgc ttggcgatcg cttcgttttg gcgcctctga
     1801 tgctcttcat ccggcttcac cgtgacaagg atccggtggg ggttgtcaag gaggtgatcc
     1861 ttgatccaat cctcgaaata ccgggggttc cgcttgatcc gtttttccag ctcgttgagg
     1921 gtcggaacca actcgatcgt ctccgagggc gaccggtcga ccatccacgc ccgggtggct
     1981 cgggtcaatg ccctgaggcc ggtgggaata ccgccgggaa tctccagctg gctgaagcgg
     2041 gtgcgtttca gcgacgtttc gatgagctcg ggatcgagac cgtcccggac gatctttttc
     2101 agcgtggaaa gaatgacgtt ctgcgccttt tcaccgtctt ccgggtcgat ccccttgaag
     2161 ccagcgatga a
//
LOCUS       DAOPUZ010000215         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_54344_length_2166_cov_3.724301, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000215 DAOPUZ010000000
VERSION     DAOPUZ010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54344_length_2166_cov_3.724301"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKN79_04240"
     CDS             complement(<1..592)
                     /locus_tag="PKN79_04240"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95006.1"
                     /translation="MIVLLLIFITSCFGCSCDIPADEHVFRVLSYNVENLFDATLDGT
                     EHAEYRDSKKWNERSYRLRLRNLGKILLDPALNHPDVIILQEIENERVVEDLLSFHLA
                     KKGYRSYAVARTEGSPISVGAISRHPIVRSSVHAVPGGRNILEIVISTPVEEVVIFAL
                     HAKSQIGGFNESELLRIENAKSVSLASRDHVDKAVLV"
     gene            complement(625..>2166)
                     /locus_tag="PKN79_04245"
     CDS             complement(625..>2166)
                     /locus_tag="PKN79_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNZ95007.1"
                     /translation="MRRRLLLKWLATGVTVIFGLTTPFFRLELGSSNGIVVLYSVLLG
                     LSVMAASWVSKRFSDRLGSKPLVVISTLFTLGFFALWAILPRTLHFAWFFGLGFLTNT
                     FVALINLLTFRLLTQVMPDDELVSFNSMVNFINGIVAFGVGMLSGFLANFTQGSLLFH
                     GTALGNGYTLVFIFGFALILVEALVALRIQEIGAYSSQAAAQVVFSMHGIRAVSMIER
                     LERTSDPAKRRMLMHNLGGNVNYLATSELRSILASPFHVDKLEAVRAIGDRPRKSLLD
                     DLIRVAQDDDSYVQLDAIAALGSYRKEEKAKSALVDLLLHGRWASVRSMASKSLARIS
                     DSDEYLNVVNELSRSAKHIDEVIDYLVAKRFMDKEGRFFQEFFIFVEQGRSGTFRQTG
                     YSVVASLLRFGPPSLASLYEERNLSSTKAYLAGFLSEARDLTMIDQNYNLIIQIFAKE
                     QWSKLIDLCLESLRSSDVQADSSLDNLKKGLLKVETMSLESFDVVDMIALLYFTYFIC
                     KSQNS"
BASE COUNT          523 a          595 c          628 g          420 t
ORIGIN      
        1 agacgaggac cgctttatcg acatggtccc tgctcgccaa ggagacgctt ttcgcattct
       61 cgattcgaag gagctcgctt tcattgaacc cccctatctg acttttggcg tgcagggcga
      121 agatgacgac ctcttcgacg ggagtcgaga tgacgatttc caatatgttc cgtcccccgg
      181 gaaccgcatg gaccgaggag cggacgatcg gatggcggct gatcgccccg acgctgatcg
      241 gcgaaccctc cgtccgggcg accgcataag agcggtaccc cttcttcgcc agatgaaagg
      301 aaagcagatc ctccacgacc cgttcattct caatctcctg aaggatgatc acatcgggat
      361 ggttgagggc cgggtcaagc aggatcttgc cgaggttcct gagccggagc cggtaggagc
      421 gctcattcca tttctttgaa tcccgatact cggcatgctc cgttccgtcg agggtcgcat
      481 cgaagaggtt ttccacgttg taggagagaa cccggaagac atgctcatcg gcgggaatat
      541 cgcacgagca tccgaaacat gaggtgatga agatgagaag aagaacgatc atgccccgta
      601 agggggaaaa gccgccgctt cgcttcagct gttctggctc ttgcagatga aataggtgaa
      661 atagaggagc gcgatcatgt cgaccacatc aaaagattcc aagctcatcg tttcaacctt
      721 cagcaatcct ttcttcaggt tgtccagact cgaatccgcc tgcacatcgc ttgaacgcag
      781 ggactccaga cagagatcga tcagcttgga ccactgctct tttgcaaaga tctgaatgat
      841 gaggttgtag ttctgatcaa tcatcgtcag atcgcgggct tcggagagga agccggcaag
      901 atacgctttc gtcgagctca ggttgcgctc ttcatacagg gaggccagac ttggcggacc
      961 gaagcgaagc agggaggcga cgaccgaata gccggtttga cggaacgtgc cgctgcgtcc
     1021 ctgttcaacg aagatgaaga actcctgaaa gaaccttcct tctttgtcca tgaagcgctt
     1081 ggcgaccaga tagtcgatca cttcgtcgat atgcttggct gaacggctga gctcattgac
     1141 cacgttgagg tactcatccg aatcgctgat ccgagcaagc gactttgacg ccatcgagcg
     1201 aaccgaagcc catcggccat ggagcagcag gtcgacgagg gcgctcttcg ccttctcttc
     1261 cttccggtag gagccgaggg cggcgattgc atcaagctga acataggagt cgtcatcctg
     1321 ggcgaccctg atcagatcat ccagcagtga ttttcgaggc cggtcgccga tcgccctgac
     1381 cgcttcaagc ttgtcgacat ggaaggggct ggcgagaatc gagcggagct cgcttgtcgc
     1441 aaggtagttc acgttccccc cgaggttgtg catcagcatc cgtcgcttgg cgggatcgct
     1501 tgtccgctcc agccgctcga tcatcgaaac cgcccggatt ccgtgcatcg agaacaccac
     1561 ctgggcggcg gcttgcgacg agtaagcccc gatctcctgg attctgagag cgaccagagc
     1621 ttcgaccagg atcagggcga aaccgaaaat gaagacgagg gtatacccgt ttccgagcgc
     1681 cgtgccatga aacagcaagc ttccttgggt gaagttcgcc aaaaacccgc tgagcattcc
     1741 gacgccgaaa gcgacgattc cgttgatgaa gttgaccatc gagttgaagg agacaagctc
     1801 atcatccggc atcacctggg tcaaaaggcg gaacgtcaag aggttgatca gcgccacaaa
     1861 ggtgttcgtg aggaacccga ggccaaagaa ccaggcgaag tggagggttc ggggaagaat
     1921 cgcccaaagg gcgaaaaacc ccaaggtgaa gagcgtcgaa ataacaacca gcggcttgga
     1981 gccgaggcgg tcggaaaaac gcttcgagac ccacgaagcg gccatcacgg aaagaccgag
     2041 caacacggaa tagagaacga cgatgccgtt ggacgagccc agttccaagc ggaagaacgg
     2101 ggtcgtgagg ccaaatatga ccgttacccc ggtggcgagc catttcagca agagccgtct
     2161 cctcat
//
LOCUS       DAOPUZ010000216         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_54358_length_2166_cov_2.487920, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000216 DAOPUZ010000000
VERSION     DAOPUZ010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54358_length_2166_cov_2.487920"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PKN79_04250"
     CDS             complement(<1..376)
                     /locus_tag="PKN79_04250"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNZ95008.1"
                     /translation="MLIAVDIGNSHIVVAVHSGKEWIHSFRIHSDQKKTTDEYFVVLE
                     ALMNNAKISRQDITKAVISSVVPNLTRAIAKNIYNLFSIQPLMVSHSVNTNLIRETIP
                     KELGMDLLANLAQAHYVHPDRCV"
     gene            complement(387..2023)
                     /locus_tag="PKN79_04255"
                     /pseudo
     CDS             complement(387..2023)
                     /locus_tag="PKN79_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606970.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2081..2152)
                     /locus_tag="PKN79_04260"
     tRNA            complement(2081..2152)
                     /locus_tag="PKN79_04260"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2117..2119),aa:Glu,seq:ctc)
BASE COUNT          453 a          584 c          591 g          538 t
ORIGIN      
        1 tcacacatct atcggggtga acgtagtggg cctgcgccaa gttggctaag aggtccatcc
       61 ccagctcctt ggggatcgtc tcgcggatca gattggtgtt cacggaatgg ctcaccatca
      121 gaggctggat cgagaagagg ttgtagatgt ttttggcgat cgcccgggtg aggttgggaa
      181 caacgctgct gatgaccgct ttggtgatat cctgacggct gatcttggcg ttgttcatca
      241 gcgcctcgag gacgacgaaa tactcgtcgg tcgtcttctt ctgatcgcta tgaatccgga
      301 acgagtggat ccactctttt ccactgtgca ccgcaacaac gatatggctg ttgccgatat
      361 caaccgctat caacatggca ccttcctcag agcgccaaag cgttcttcac tcctccgtag
      421 agttcggttc ggagcgcctt gattccctcg tccttaaaat actcttcaaa gctcatcatc
      481 ctgtcaatca ctcctcccgg agtgaattcg atgatccgat tcgcgatcga ttcgacgaac
      541 tgatggtcat ggctgttgaa cagaatattt ccggtgaatt caatcagccc gttgttgagg
      601 gccgtgatcg cctcaaggtc caagtggctg gtcggttcat cgaagatcag gacgttcgcc
      661 ccctcaagca tcatcttcgc aaggagacag cggaccttct ctcccccgct taacacggta
      721 agatccttca gtgcttcatc accgctgaag agcatccgcc cgaggaagga acggacgtag
      781 gtctcatcct ggtcgggact gtaccggcgg agccaatcca cgatcggaac atgctcctca
      841 aagagatggt tgttgttctt cggaaagtac gaaaggctcg tggtgacgcc ccaggtgtag
      901 gtaccgccgt cctgtttgtc attgccgctg aggatctcga agagggcgct cttggcggcg
      961 tggttcggcc cgacgaacgc gatcttgtcc ccggcgttca cgtgaagaga gaatttgccg
     1021 agcagcacct ctccatcgat cgatttggaa agggagtcga catccagaat attccggcct
     1081 acctcccgat tgggcttgaa ggcgacatag ggaaagcggc ggctggaggg acggatatca
     1141 tcgatcgtga gcttgtcgat cagtttcttt cggctggtcg cttgacgggc tttggcgaca
     1201 ttggaactga agcggtagat gaactccttc agttcggcga tcttttcctc ccgtctccgt
     1261 ttctcgtctc gctgctgttt tgcggcaagc tggctggaaa gataccaaaa gtcgtagttg
     1321 ccgacgtaca gctggatctt tccgtaatca atgtcggcga tatgggtgca caccgtattg
     1381 aggaagtgcc ggtcgtggga gaccacgatc acggtgttgt cgaagtcgga aaggaaatcc
     1441 tcaagccagt ggatcgactc caggtccaag tggttcgtcg gctcgtcaag gaggagaata
     1501 tcgggattcc cgaacagcgc ttgggccaac agagcccgga ctttgatcga gtcttccact
     1561 tcagccatct gtttttcatg gagcgcatcg gcgattccga gtccgctgag cagttgagcc
     1621 gcttcgctct ccgcttccca ccctccgagt tcggcgaaat cgccttccag ctcggcggcg
     1681 cgcagtccgt cctcttcggt aaactcctct ttggcataga tctcatcccg ttctttcatg
     1741 attgaataga gcttttcata ccccataatg accgtatcca gaaccgagta ctcgttgaac
     1801 gcgaaatggt cctgacgaag ggtggcgagc cgctgcccgg ctgtgacgat gatcttgccg
     1861 gtgtccggtt cgatttcacc gctgagaatc ttaaaaacgt ggattttccc gcaccgttcg
     1921 ctccaatgat cccataacag tttccggggg taaacttgat gtctacctcc ttgaagagaa
     1981 cttgcgtgcc gtatgacagg cctatgttgg atgctgtaat catcgatgtt tcctctgtcg
     2041 gaaaaacagg gcaaccggtg gttgccctgc ttcacactcg tagttcctag gggaatcgaa
     2101 cccctatttg aagactgaga atctcccgtc ctaaccgtta gacgaaggaa ccgattgctg
     2161 ggatgg
//
LOCUS       DAOPUZ010000217         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_54392_length_2165_cov_2.816588, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000217 DAOPUZ010000000
VERSION     DAOPUZ010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54392_length_2165_cov_2.816588"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PKN79_04265"
     CDS             complement(<1..534)
                     /locus_tag="PKN79_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNZ95009.1"
                     /translation="MNMHEKIKARAKELRSYTAENLSKMVKTKSYSSQEEDVARLIVT
                     LLEEAGFDEVYIDGLGSVIGRVGNGPKKLAFDAHIDTVEVGNPDLWNFDPFSGEIKDG
                     IVYGRGASDQKGGAASMITAGRILKELGYGGEYTVYFTFTVMEEDCDGMCWKYLIEEE
                     GLKPDLVVSTEPTSCRLY"
     gene            complement(576..1898)
                     /gene="ssnA"
                     /locus_tag="PKN79_04270"
     CDS             complement(576..1898)
                     /gene="ssnA"
                     /locus_tag="PKN79_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HNZ95010.1"
                     /translation="MADLVIKNTRIMQTEPPFQVLEGVDIVIDGNTITKVGRDAAKGV
                     AAKKVIDGTDKTVMPGNVCSHHHYYSGLSRGMLIQAGPQRDFIQVLKEWWWRLDRGLD
                     EEACYYSSLICSLDAIMAGTTAAIDHHASPSYIAGSLDTIAKGMEKVGIRGSTCYEIT
                     DRNGGMAEVEAGVEESRRFIERSKKSSLVKAMIGGHAPFTIPNEGLKLMGDLVEKTKV
                     GIHLHVAEDRYDVAHSHHRYGMDIVDRLDEYGLLTDNALLVHGLYLNEDEIRKINERG
                     VFFAHNPRSNMNNHVGYCSHIQDVKNLLIGTDGCGGNMFEELKIGFFKHKDEGLSWWP
                     PQYVEALNRGYRLVEKYFDGSFGRVEAGMVADLVITDYHNPTPLVQENAASHFIWGMS
                     SNCVESVIVDGKVVMENRAFAHLDVDEIYREAAKVAKRVWKEVDTIAP"
     gene            complement(1902..>2165)
                     /locus_tag="PKN79_04275"
     CDS             complement(1902..>2165)
                     /locus_tag="PKN79_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95011.1"
                     /translation="FCPYDGGPYKKKFTLFSRRDDFENSTNSGFLVEDGSILIRVDGK
                     LYPCELDKDGVLVGEEEGLSDEVAAIVEEVFASYSYLLGEVEE"
BASE COUNT          402 a          672 c          578 g          513 t
ORIGIN      
        1 atagaggcgg cagctcgtcg gctcggtgga aacgaccaga tcgggcttga ggccttcctc
       61 ttcgatcaga tacttccagc acattccgtc acagtcctct tccatcacgg tgaaggtaaa
      121 gtacacggta tactcgccgc cgtagcccag ctccttgagg attcgaccgg cggtgatcat
      181 tgaagccgcc ccgcccttct ggtcggatgc gcctcgaccg tagacgatac cgtccttgat
      241 ctccccgctg aacgggtcga aattccacag atcggggttt cccacttcaa cggtgtcaat
      301 atgagcatca aaggccagct tcttcggccc gtttcccact cggccgatca ccgagccgag
      361 gccgtcgatg tatacctcat caaagccggc ttcctccagc agggtaacaa tcaggcgggc
      421 tacatcttcc tcttgggagc tgtagctctt cgtcttcacc attttcgaga ggttctcagc
      481 ggtgtaggag cggagttctt tcgctcgagc tttgatcttt tcatgcatgt tcatcgttgt
      541 acctaccttc cttttcgtat atattcttcg ccttgttacg gggcgatagt gtccacttct
      601 ttccatactc gcttggcgac cttcgccgcc tcgcggtaga tctcatccac atcgagatgg
      661 gcgaaggctc gattctccat cacgaccttc ccatcgacga tgacgctctc gacgcagttg
      721 ctgctcatcc cccagatgaa gtgactggcg gcattctcct gaacgagcgg ggtcgggttg
      781 tggtaatcgg tgatgaccag atcggcgacc atccccgcct cgactcgccc gaaggagccg
      841 tcgaagtatt tctccaccag ccgatagccc cggttcagcg cctcgacgta ctgcggcggc
      901 caccaggaga ggccttcgtc cttgtgcttg aagaatccga tcttcagctc ctcgaacata
      961 ttcccgccgc agccgtccgt tccgatcaga aggttcttca catcctgaat gtgggagcag
     1021 tagccgacgt ggttgttcat gttcgatcgg gggttgtggg cgaagaacac ccctcgttca
     1081 ttgatcttcc tgatctcgtc ctcattcaga tagaggccgt gaaccaagag ggcgttatcc
     1141 gtcaggagcc catactcgtc gagccggtcg acgatatcca tcccgtaccg gtggtgactg
     1201 tgggccacgt cgtaccgatc ctcggcgacg tggaggtgga ttcccacctt cgtcttctca
     1261 acgaggtcgc ccatcagctt caatccctca ttggggatcg tgaagggggc gtgtcccccg
     1321 atcatcgcct tgacgaggct gctcttcttc gagcgctcga tgaagcggcg actttcctcc
     1381 acccccgcct cgacctcggc cattccgccg ttgcggtcgg tgatttcata acaggtggat
     1441 ccgcggatac cgaccttctc catccccttg gcgatcgtgt ccagagagcc ggcgatatag
     1501 gagggactgg cgtggtggtc gatcgcggcg gtcgtccctg ccatgatcgc atcaagggag
     1561 cagatcaggg agctgtagta gcacgcctcc tcatccagac cgcggtccag acgccaccac
     1621 cactccttga gaacctggat gaagtccctt tggggtccgg cctggatcag catcccccgt
     1681 gaaagtccgc tgtaatagtg gtggtgcgag cagacgttgc ccggcatcac cgttttatcg
     1741 gttccgtcga tcaccttctt ggcggcaacc cccttggcgg catccctgcc gaccttggtg
     1801 atggtgtttc catcgatgac gatatcgacg ccctccaaaa cttggaacgg gggctcggtc
     1861 tgcatgattc gggtattctt gattacgaga tctgccattt cttactcctc aacctctccc
     1921 aggaggtagc tgtaggaggc aaacacctcc tcgacgatcg cggcgacctc atcactgagg
     1981 ccttcctctt caccgacgag cactccgtcc ttatccagtt cacacggata gagcttgccg
     2041 tcgacccgga tcagaatcga accgtcttcg acgaggaagc cgctgttggt ggagttctcg
     2101 aagtcgtccc gacgactgaa gagggtgaac ttcttcttat agggcccgcc gtcatacgga
     2161 cagaa
//
LOCUS       DAOPUZ010000218         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_54394_length_2165_cov_2.756872, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000218 DAOPUZ010000000
VERSION     DAOPUZ010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54394_length_2165_cov_2.756872"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1298)
                     /gene="thrC"
                     /locus_tag="PKN79_04280"
     CDS             complement(30..1298)
                     /gene="thrC"
                     /locus_tag="PKN79_04280"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNZ95012.1"
                     /translation="MKFISTRGNAEPIGASQAIMQGLAPDGGLYIPESFPSLAHVNVH
                     EISSYPELAYEVLAPFFNGDPLAPYLAEICVNAFTFPAPLVQLEEGRFVLELFHGPTA
                     AFKDFGARFLAYSMEQLLQLQDRKLTILVATSGDTGGAVAAAFAGRKGIDVKVLFPKN
                     RVSARQQHQLTCWGGNIEAYEVDGSFDDCQAMVKAAFMDKEISRRWGLSSANSINLGR
                     LLPQMVYYVYGAVRVQAASGKKPHFIIPSGNVGNSCGAYWAKTMGAPIESITLALNAN
                     RTIVDYLKSGEYAPRASIETLANAMDVGSPSNMERLFALFGDLATMRLNVSAHSVDDP
                     AIRQTIKEVYEQYGYIICPHTATGVRVQKDFVSESPSIVVSTAHPAKFETVVEPLIGH
                     VLDVPPALEEILKKETRMRSIGTDYLELFQ"
     gene            complement(1310..>2165)
                     /locus_tag="PKN79_04285"
     CDS             complement(1310..>2165)
                     /locus_tag="PKN79_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HNZ95013.1"
                     /translation="FAAHISATYFPHSLIIDCSTSSELASHYVGWLKDRIHVITPNKK
                     AGTAEYSYYKELMDACLSTGSRFLYETTVGAGLPVIWTLKDLVQTGDHVHRIEGMLSG
                     TLAWLFSTYDGSVPFSTLVRQAKEMGYTEPDPRDDLSGMDVGRKTVILARELGGKIEV
                     ADVPIQSLVPESLEGVGLDEFMERLEEIDPIIEQAYRTARSKGEFLRYVGTVDEEGNC
                     EARLGSFGPDHPFSQATGTDNVILFVTDRYRTQPLVIKGPGAGREVTAGGVFSDILRL
                     SSYLGARI"
BASE COUNT          443 a          648 c          625 g          449 t
ORIGIN      
        1 attcggaagg cgccgaccac cgcgctgatc tactgaaaca gctcgaggta gtcggtcccg
       61 atcgatctca tgcgcgtctc ttttttcaga atctcttcaa gagcgggcgg cacgtcaaga
      121 acgtgcccga tgaggggctc aaccaccgtt tcaaacttcg ccgggtgggc ggtgctcacc
      181 acgatcgaag gggattccga aacgaagtct ttttgaaccc gcactccggt ggcggtatgg
      241 gggcagatga tgtacccata ctgctcatac acttccttga tcgtctgccg gatcgccgga
      301 tcgtcaacgc tgtgggcgct cacgttcagg cgcatcgtcg caaggtcgcc gaagagcgcg
      361 aaaagccgct ccatgttgct cggagagccg acatccatcg cgttggccag cgtttcgatt
      421 gaagcgcggg gggcatactc gccgctcttc aggtaatcga cgatcgtccg gttggcgttc
      481 agggcgaggg tgatcgattc gattggagct cccatcgtct tcgcccagta cgccccgcag
      541 ctgttcccca cgtttccgct ggggatgatg aagtggggct tcttgccact cgccgcctgg
      601 acccgaacag caccgtagac gtaatagacc atctgaggga gaagccgtcc gaggttgatc
      661 gaattagcgc tggacaggcc ccatcgcctg ctgatctcct tgtccatgaa ggcggctttg
      721 accatcgcct gacaatcgtc gaaggaaccg tcgacctcat acgcttcgat gtttccgccc
      781 cagcaggtca gctggtgctg ctggcgcgca ctcacccgat tcttcggaaa gagcactttg
      841 acgtcgatcc cttttcgacc ggcgaacgcc gcggccaccg ccccgccggt atcaccgctg
      901 gtggcgacca ggatcgtaag cttccgatcc tgaagctgca gcagctgctc catcgagtac
      961 gctaaaaacc gcgccccgaa atccttgaac gccgcagtcg gtccgtgaaa cagttcaagg
     1021 acgaaccgcc cttcctccaa ttgaacgagg ggtgcgggaa acgtaaacgc gttgacgcag
     1081 atctcagcca gatagggagc gagcggatct ccattgaaga agggagcgag cacctcatag
     1141 gcaagctcgg gataggaaga gatctcatgg acattcacat gagcaagaga gggaaaggat
     1201 tcggggatgt agagcccccc atccggagcc aagccctgca tgatcgcctg acttgctccg
     1261 atcggttcgg cattgccgcg cgttgaaatg aacttcatcg tccgttctcc taaatccggg
     1321 cgcccagata ggatgagagg cgaaggatgt cgctgaagac tcctcctgcg gtcacttccc
     1381 tgccggctcc cggtcctttg atcacaagcg gctgggtccg atagcgatcg gtgacgaaaa
     1441 ggatcacgtt gtccgtcccc gtcgcctgac tgaagggatg gtccggtccg aatgaaccga
     1501 ggcgggcttc gcagttcccc tcttcatcca ccgttccgac gtaacggagg aactctccct
     1561 ttgagcgagc ggtccggtac gcttgttcga tgatcggatc gatctcctcc agccgctcca
     1621 tgaactcgtc caggccgact ccttcaaggg attcgggaac gaggctttga atgggcacat
     1681 cggccacttc gatcttgccg cccagctcgc gggcgagaat caccgtcttt cgccccacat
     1741 ccatccccga aagatcgtct cgaggatcgg gttcggtgta tcccatctcc ttcgcctgac
     1801 ggaccaaggt cgaaaacgga accgatccgt cataggtgga gaagagccag gcgagggtac
     1861 cgctgagcat cccttcgatc cggtggacat gatcaccggt ctgcaccagg tctttcaggg
     1921 tccagatgac cggaagcccc gcccccaccg tcgtttcata gaggaatcgg ctgccggtgc
     1981 tcagacatgc atccatcaat tccttgtagt aggagtattc ggcggttccg gctttcttgt
     2041 tgggcgtgat gacgtggatg cggtctttca accatccgac ataatggctc gccaattcgc
     2101 tgctcgtcga gcaatcgatg atcagggagt gagggaagta tgtcgcactg atatgggcgg
     2161 caaac
//
LOCUS       DAOPUZ010000219         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_54541_length_2161_cov_3.860399, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000219 DAOPUZ010000000
VERSION     DAOPUZ010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54541_length_2161_cov_3.860399"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PKN79_04290"
     CDS             <1..959
                     /locus_tag="PKN79_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNZ95014.1"
                     /translation="ATTLGIVGLISFLFTMVLLINKVWSVINQIYRSAQSTNPFKRFM
                     GYMTFVIITTLLLALYVSVQSVFTDWYLNLIGYSVGAWSVVIKKVIPVLIIFIIFFLL
                     IFFVPNTKVQFSAALIGSVSGVVIITVFTQFTGLLTSRAATFSVIYGSFAALFMFLFI
                     CYVFWATIFFSVELAYVYQYRPTGVVSEGLPQSPATQMTDGTNIMMVIGANFRDGKGA
                     TTVKELLDRLVIPYDRLQGFLGALTNQGYIVATNNSRTSFIPNKPLDQIRLQDLVEHL
                     YGIETIDEMSRDTAGEAVAKQLAEKGISSLGTLTVENLLQRI"
     gene            complement(956..1774)
                     /locus_tag="PKN79_04295"
     CDS             complement(956..1774)
                     /locus_tag="PKN79_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNZ95015.1"
                     /translation="MLKHPIILAFLATMGTWGMTAIGAAFVFFFKTINRHVMNGMLGF
                     ASGVMIAASFWSLLQPAIELAEGGSLPPWAVAAIGFLAGGAFLWLSDQLIPHAHLDSK
                     PGESEGIPTNLRRSILLVLAITLHNIPEGLAVGVAIGAAAAGSGSQTMTGALIVAIGI
                     GLQNLPEGAAVSVPLRREGLSRRKSFFYGQASGIVEPIAGVLGAWLVTTMTPILPYAL
                     AFAAGAMIYVVIEELIPESQQVYGTADDHSATHWATIGTMLGFTIMMILDVALG"
     gene            1859..>2161
                     /locus_tag="PKN79_04300"
     CDS             1859..>2161
                     /locus_tag="PKN79_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95016.1"
                     /translation="MIFLEYETLEPIDALLWHAYPKHESIMETYDVGELTVEVLDHPS
                     LRSSIDLAVIAFSLLVFHNNEIIAVFQIEQEDLRSLAEKLGCSIRELQDEYRTKGML"
BASE COUNT          448 a          619 c          606 g          488 t
ORIGIN      
        1 acgccaccac gctgggaatc gtcggactga tctctttttt gttcacgatg gtgcttctga
       61 tcaacaaggt ctggtcggtg atcaaccaga tctatcgctc cgcccagtcg accaatccct
      121 tcaaacgctt catgggatac atgaccttcg tcatcatcac gacgctgctt ctcgccctct
      181 atgtgagcgt ccaatcggtg ttcacggatt ggtatctgaa tctcatcggg tattcggtcg
      241 gcgcctggtc ggtggtcatc aagaaggtga ttcccgtcct gatcatcttc atcatcttct
      301 tcctgctgat cttcttcgtt ccgaacacca aggtccagtt cagcgccgcg ttgatcggca
      361 gcgtctcggg cgtcgtgatc atcaccgtct ttacacagtt caccggcctg ttgaccagtc
      421 gggcggcgac gttcagcgtg atctacggat cgtttgccgc cttgttcatg tttctgttta
      481 tctgttatgt gttctgggcg acgatctttt tcagcgtcga gctggcatat gtgtatcaat
      541 atcgaccaac cggggtggtc agtgaaggcc ttccgcagag cccggcgacc cagatgaccg
      601 acggtaccaa catcatgatg gtgatcggtg cgaacttccg ggacggcaag ggtgccacga
      661 cggtcaagga gctcctcgac cgattggtca ttccatacga tcggcttcag gggttcctcg
      721 gcgctcttac aaaccaaggg tacatcgtcg cgacgaacaa cagccggaca agcttcattc
      781 ccaacaaacc ccttgatcag atccggctcc aggatcttgt cgaacacctc tacggaatcg
      841 aaacgatcga tgagatgagc cgggataccg ccggagaggc ggtcgcaaaa cagcttgccg
      901 agaaagggat ctcctcgctc ggcaccttga cggttgagaa tctgctccag cggatctagc
      961 cgagggcgac atccaaaatc atcatgatgg tgaagccgag catcgtgccg atcgtcgccc
     1021 agtgggtggc cgagtggtca tccgccgttc catagacttg ctgtgactcg gggatcagct
     1081 cttcaatgac cacgtagatc atcgctccgg cggcgaacgc aagggcgtag gggaggatcg
     1141 gggtcatcgt ggtgaccagc caggctccga ggactccggc gatcggttca acgattccgg
     1201 aggcctgacc gtagaagaag ctctttctgc gggaaagccc ctctcttcgc aaggggacgg
     1261 aaaccgctgc cccttcgggg aggttctgca agccgattcc gatcgccacg atcaacgctc
     1321 cggtcatcgt ctgggatccg cttcccgccg ccgctgctcc gatcgccaca ccgacggcga
     1381 ggccttcggg gatattgtgc agggtgattg ccaaaaccaa cagaatcgag cggcgaaggt
     1441 tggtggggat tccttcgctt tcacccggtt ttgagtccag gtgggcgtgg gggatcaatt
     1501 ggtcactgag ccagaggaac gccccgccgg cgagaaaccc gatcgcggcg accgcccacg
     1561 gaggaaggga tcctccttcg gcgagttcga tcgccggctg gagtagcgac cagaatgagg
     1621 ccgcgatcat cacgccgctg gcgaagccca gcatgccgtt catgacgtgg cggttgatcg
     1681 tcttgaagaa gaagacgaac gccgccccga tcgcggtcat tccccaggtt cccatggtcg
     1741 ccagaaatgc taatatgatt ggatgtttca gcatagagat atgctacggg tacgcccttc
     1801 tccggtcaag tggggatatc tcttgcctaa agggagttct tcccattatg gttgaacaat
     1861 gatcttcctt gagtacgaaa cccttgaacc gatcgacgcc ctgctttggc atgcataccc
     1921 gaagcatgag tcgatcatgg aaacctatga tgtcggagaa ctcaccgtcg aggtgctcga
     1981 tcatccgagt cttcgttcct cgatcgatct cgccgtcatc gcgttttcac ttctcgtgtt
     2041 ccataacaat gaaatcatcg ccgtcttcca gatcgagcag gaggatctgc gctcccttgc
     2101 tgaaaaactg gggtgcagca tccgcgagct tcaggatgaa taccggacga aaggaatgct
     2161 g
//
LOCUS       DAOPUZ010000220         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_54831_length_2154_cov_2.804669, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000220 DAOPUZ010000000
VERSION     DAOPUZ010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54831_length_2154_cov_2.804669"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKN79_04305"
     CDS             <1..397
                     /locus_tag="PKN79_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95017.1"
                     /translation="APPAADALAALSCANAGPATSATPIEMDIKLRTWVITFIEEAGE
                     TLRTKQVLEVLQNLSIQTVNIALRGVEIVDRWIRDDGTVYVLASYPVKNLKEAYRLQA
                     EELERKALADAIKADVLETYLQGQLEKVD"
     gene            485..859
                     /locus_tag="PKN79_04310"
     CDS             485..859
                     /locus_tag="PKN79_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNZ95018.1"
                     /translation="MNRIILYALILMILFLIVKKVGGGARISAKDVYALLQSDEEVIL
                     VDVRTEGEYYSGHIDGAIHLSLYDIGAEKPALLSDLDAPIIVYCQSGARSGIARRKLK
                     ALGYTNVRDMGGLMFWRYDLVK"
     gene            869..1492
                     /locus_tag="PKN79_04315"
     CDS             869..1492
                     /locus_tag="PKN79_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95019.1"
                     /translation="MHRVIVLKRLDELTVVDDGTIYHGFDQEWYSKLWQRRAGCGPTT
                     ASTLIRYNAKRNKSRTKASSIRLMEELWSFVTPGIMGVHTVAHFTRGLREYLVKKHLG
                     FEIAALSVPIDRANRPSFAQVREFLVSRLEADQPVAFLNLNNGKVENLDPWHWVTIVG
                     IEQRGADRPLLAHVYDGGRKLVINLTLWYETTTRPGGFVSLVEGGEG"
     gene            1553..>2154
                     /locus_tag="PKN79_04320"
     CDS             1553..>2154
                     /locus_tag="PKN79_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ95020.1"
                     /translation="MVYSANEQRYEKMQYARTGNSGLKLPRISLGLWHNYGDAYPISN
                     GRAMLRTAFDLGINHFDLANNYGPPAGSAEENFGKILKQDFAPYRDELIISTKAGYYM
                     WPGPYGEWGSRKYLLSSLDASLKRMGLEYVDIFYSHRFDPNTPLEETMGALVSAYRSG
                     KALYVGISSYTAEQTRKAYAILKDMGVPLLIHQPSYSMLNR"
BASE COUNT          527 a          519 c          629 g          479 t
ORIGIN      
        1 agctccgcca gcggctgatg cgttggcggc gctgtcctgc gcgaacgcgg ggccggcgac
       61 gagcgccaca ccgattgaga tggatatcaa gctgcgcacc tgggtgatca ccttcattga
      121 agaagcgggt gaaaccctga ggaccaagca agtcttggag gtgcttcaaa acctttccat
      181 tcagaccgtg aacatcgccc tgcggggggt ggagatcgtc gatcgatgga tccgggatga
      241 cggaacggtc tatgtgctgg cttcctatcc ggtaaaaaac ctcaaagagg cgtatcgcct
      301 tcaagccgag gagcttgagc gaaaagcctt agccgatgca atcaaggcgg acgtgctgga
      361 aacatatttg caaggccaac tggagaaagt cgactaggga aagcaatcga gtgaatcgac
      421 caccactgaa cgttgacgtt ggtggtggtt ttttgcaatc tttacacaca ggagtttttc
      481 agcaatgaac agaataatac tctacgcgct catattgatg attctcttcc tcatcgtcaa
      541 aaaagtgggt gggggagctc gaatctccgc caaggatgtc tatgcactgc ttcagagcga
      601 tgaagaggtg atcctggtgg atgtccggac tgagggtgag tattacagcg gacatatcga
      661 tggagcgatt catctttccc tatatgacat cggagcggaa aaacctgcat tgttgagtga
      721 tctcgatgct ccgatcatcg tatattgcca gagcggagcc cgcagcggca tcgccaggag
      781 gaaactgaaa gcgttggggt atacaaacgt tcgcgatatg ggtggtttga tgttttggcg
      841 gtatgatttg gtgaagtagg gaaagagcat gcatcgagtg attgtcctga agcgtttgga
      901 tgagctgaca gttgtagatg acgggacaat ctatcacggt ttcgatcagg aatggtactc
      961 caagctttgg cagcgaagag cggggtgcgg gccgacgacc gcaagcactc tgatccgcta
     1021 caatgcaaaa cgaaacaaaa gcagaacgaa agcgtcctca atccggctga tggaggagct
     1081 ttggagcttt gtcactcccg gaatcatggg cgtccacaca gtcgcccact tcacccgggg
     1141 attgagggaa tatcttgtca aaaaacactt gggctttgag attgccgccc tgtcggtgcc
     1201 gatcgatcga gccaatcgtc cttcgttcgc acaagttcgt gaattcctcg tttcccgttt
     1261 ggaagccgat cagccggtag cgtttttgaa tctgaacaac ggaaaggtgg aaaacctcga
     1321 tccctggcac tgggttacga tcgtggggat cgaacagcgg ggggcggaca gaccgcttct
     1381 tgcccacgtg tacgacgggg gaagaaagct cgtgatcaat ctcaccctct ggtatgagac
     1441 aaccacccgc ccggggggat tcgtcagcct cgtggagggc ggcgaaggtt gaaggagctg
     1501 cggtagctcc cgtttcttcg gtggtgtata ctgacagaga aggagaacgg tcatggtata
     1561 ttcagcaaat gagcaacgat atgaaaagat gcagtacgct cgaacgggaa acagcgggct
     1621 caagcttccc cggatttcgc tcggactttg gcacaactac ggtgatgcat acccgatttc
     1681 caacgggaga gcgatgcttc gaaccgcttt tgatctgggg atcaaccact tcgatctggc
     1741 caacaactac ggaccgcccg ccggctcggc ggaggagaac tttggcaaga tcctgaaaca
     1801 ggacttcgcc ccgtatcggg atgagctcat catctccacg aaagccggat actatatgtg
     1861 gccaggtcct tacggggagt gggggagccg gaaatacctt ctttcaagtc tggatgcaag
     1921 cttgaaacga atggggcttg agtacgtgga tatcttctac tctcaccgct tcgatcccaa
     1981 tactcccttg gaggagacga tgggggcttt ggtcagtgcg tataggagcg gcaaggcgct
     2041 ctatgtgggg atctcctcct acacagcgga acagactcga aaagcatacg ctattctgaa
     2101 agatatgggt gttcccttgt tgatccatca accctcctac tcaatgttga atcg
//
LOCUS       DAOPUZ010000221         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_55153_length_2145_cov_3.589474, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000221 DAOPUZ010000000
VERSION     DAOPUZ010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55153_length_2145_cov_3.589474"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKN79_04325"
     CDS             complement(<1..391)
                     /locus_tag="PKN79_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ95021.1"
                     /translation="MRKPTTFLIVDDHPLFRQGLVSVVEDNPHYMVIGQAASIAEAMD
                     LLEAEEPDMMLIDISLGEESGLELVKNVKALKPHIMMLVISMYDEVIYASTSLKAGAR
                     GYLMKQEAAKTLMGAIEEILKGKIYLSD"
     gene            complement(395..2077)
                     /locus_tag="PKN79_04330"
     CDS             complement(395..2077)
                     /locus_tag="PKN79_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ95022.1"
                     /translation="MNVPDDVSVVGFDGIEASRYTQPPLTTVIQPMHNLGTNAVAILE
                     RMIDGGSEEHVVLSSAVTYRESCGCAPTFRFDSTVTRIPSYATQGEERTINEFAALLR
                     MKNPKGLITQLNRAIDATIAESGVVERWHVYLAVIENLIEAEDEKHRMMISDVIASAR
                     VFVGERGSRYQASKRVALQSSFETLRRVSAMLAGTFELEALFTNLKQGLALFGITEGY
                     LVEFTPDEGSARLLIDLSGESFEKESSPLEVEYKQLLPSVYKPSFHKGRWILMPLVHG
                     SEPLGYLIVPSGVVSPALYDVLQEQISSSLKGTLLLEQIKSHEQTLIEQVALRTKDLI
                     RTNKNLSNEIKRRIELEQEVMEISAKTMERIGQELHDDLAQHLLGISLIAASARKSIN
                     ADNEKLDSSLEQISLLLAESISKLKTISRGLLPLEKDEKTFTKRIEALVADTQRYAKI
                     DIGIDADPDFEITDGDRALHVFRIIQEALTNAVKHSKSKNVVIKLERSEDEIGRVHLQ
                     ASVEDNGIGFAKNIRKSALGLRIMRSRASMANATLDIQSSDEGTRVSVCLEE"
BASE COUNT          439 a          580 c          582 g          544 t
ORIGIN      
        1 catcggaaag atagatcttg cccttgagaa tctcctcgat cgctcccatc agcgtttttg
       61 cggcctcctg cttcatcaaa tatccccggg ctcccgcttt cagggaggtg gaggcgtaga
      121 tcacttcatc gtacatcgag atgacgagca tcatgatatg cggcttgagc gcttttacat
      181 tcttcaccag ttccagaccg ctctcctcac cgagggagat gtcaatcagc atcatgtccg
      241 gctcttcagc ttcaaggagg tccatcgctt ccgcaatcga tgcggcttga cctatcacca
      301 tataatgagg attgtcttcc acgacactga ccaatccctg gcggaagagg ggatggtcat
      361 caacaatcaa aaacgtagtg ggctttctca ccggctactc ctctaaacat actgaaactc
      421 gggttccttc gtcagatgat tggatatcga gtgtcgcatt ggccattgaa gcccgactgc
      481 gcatgattct taggcccaat gcgcttttac gaatattctt cgcaaacccg atgccattgt
      541 cttcaacgga tgcttggagg tgtacccggc cgatttcatc ttcacttcgt tcaagcttga
      601 tgacgacatt ttttgacttg gagtgtttta ccgcattggt caacgcttcc tgaatgatcc
      661 tgaagacgtg caacgcccgg tcgccgtcgg taatttcaaa gtccggatcg gcatcgatgc
      721 cgatgtctat cttggcatat cgctgcgtgt cggcaacgag cgcctcgatc cgcttggtga
      781 aggtcttttc atctttctcg agggggagca ggccccggct gatcgtcttg agtttgctga
      841 tcgattcggc caacaggagg gatatctgct caagcgagga gtcgagtttt tcattgtcgg
      901 cgttgatgct tttccgggcg ctggcggcga tcagggagat tccaagcaga tgctgggcca
      961 gatcgtcatg gagttcctgg ccgattcgct ccatcgtctt ggcgctgatc tccatgactt
     1021 cctgttccag ttctattcgc cgtttgatct cattggagag attcttgttc gttcgaatca
     1081 agtcttttgt tcgaagggct acctgttcga tcagggtctg ttcatggctc tttatctgct
     1141 caagcagcaa ggtacccttc aggctgctgg agatctgctc ctgcaggacg tcgtaaaggg
     1201 ccgggctcac cacgcccgag ggaacgatca aatatcccag cggctcgctt ccgtgcacca
     1261 gaggcatgag gatccaccgg cctttgtgga acgatggctt gtatacggaa ggaagtagct
     1321 gtttatactc cacttcaagg ggagaggatt ctttttcaaa gctctccccg gagaggtcga
     1381 tcaaaagtcg ggctgaaccc tcatccggag tgaactcgac caagtacccc tcggtgattc
     1441 cgaagagggc aagaccttgc ttgagattgg tgaagagagc ttccaactca aaggtgccgg
     1501 cgagcatcgc actgaccctc ctaagcgttt caaaggagct ttggagagcg acccgcttgc
     1561 tcgcctggta ccggctgccg cgctccccca cgaacacccg ggcggaggcg atgacgtcgg
     1621 agatcatcat tcgatgcttt tcgtcctccg cctcgatgag gttttcaatg actgcgaggt
     1681 acacgtgcca acgctccaca acaccgcttt cagcgatcgt tgcatcgatc gcccggttca
     1741 gttgggtaat aagccctttg gggtttttca ttctcagcaa ggcggcgaac tcgttgatcg
     1801 tccgttcttc accttgggtt gcataggagg gaatccgggt gactgtcgag tcaaagcgga
     1861 atgtcggagc gcacccgcag gattcacggt acgtcaccgc cgaggagaga accacatgct
     1921 cctcgctgcc gccgtcgatc atccgctcca ggatcgctac ggcatttgtt cccaggttgt
     1981 gcatcggttg aatgacggtt gtcagcgggg gttgggtgta gcgtgacgcc tcgatgccgt
     2041 cgaacccgac gacggaaaca tcgtcgggaa cgttgatcga gtactttgcc agtgagcttc
     2101 acaccatcac gtccggtatg gagcattagg tggtgagagt gaagt
//
LOCUS       DAOPUZ010000222         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_55449_length_2138_cov_3.307249, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000222 DAOPUZ010000000
VERSION     DAOPUZ010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55449_length_2138_cov_3.307249"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKN79_04335"
     CDS             complement(<1..815)
                     /locus_tag="PKN79_04335"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNZ95023.1"
                     /translation="MMGYGSKRLLTLRVLGDSLAVILAWYLAGILRFYVIWGGVLPAL
                     PSFLSLVWLVWVLNIFFISQRGLYKESLGQSWRKETGDLFIASSNAFLFLLAVLYFFY
                     DNKFSRIAIGLYYLFAIVLLVFVRTVLATYISASYRKGRFTRNILLVGYGTRLEMYEE
                     ALHSKEIKGIRVVGQYDGQVQPIKDVKPLEGETLRAVVDAVKPDLVVIGYPSKAQEKQ
                     DAMIAQGLNLLTERVVVLPVIPESYAGTKISDFKWIPQLALNAAEIGVFERMM"
     gene            complement(873..1160)
                     /locus_tag="PKN79_04340"
     CDS             complement(873..1160)
                     /locus_tag="PKN79_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HNZ95024.1"
                     /translation="MDESFEDLLKLFAKTTDHDDMRQLLEELLTPNELKDLILRWNLM
                     KDLYRGKPQREIAATYGISLCKITRGSKILKQKDAYTKRILSDRYDDHLRI"
     gene            1269..>2138
                     /locus_tag="PKN79_04345"
     CDS             1269..>2138
                     /locus_tag="PKN79_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNZ95025.1"
                     /translation="MKTTTLTPQRMKAILAMQQKEITEYEIYTRVANRVKDEENRKIL
                     KKIGEEERRHAATWAKVTGKTLKPQMGKVRFYSFLARILGYTFVLKKMEMGEEKAIEG
                     YSHILDEMPQAKEIYEDEQKHEEALIAMLDEERLQYVGSMVLGLNDALVELSGTLAGL
                     TFALKNTRLIALSGLITGISATLSMASSEYLSSKNRNEENAGKSAMYTGVAYLIAVAA
                     MVLPYLLLSNYLVALGTMLAVVLLIIVLFTYYTSVAKDLPFGPRFREMALISIGVSGI
                     SFVIGILVKNWLGI"
BASE COUNT          528 a          596 c          549 g          465 t
ORIGIN      
        1 ttcatcatcc gctcgaacac tccgatctcg gcagcgttga gggcgagctg ggggatccat
       61 ttgaagtcgc tgatcttcgt tccggcgtag gattcgggaa tcacgggaag gacgaccacc
      121 cgttcggtga gcagattgag cccctgagcg atcatggcat cctgcttctc ctgcgccttt
      181 gacggatatc cgatcaccac aagatccggc ttcacggcat ccaccaccgc acggagcgtt
      241 tccccctcca acggtttgac gtctttgatc ggctgaactt gcccgtcata ctgaccgaca
      301 acccgaattc ccttgatctc cttggagtgc agggcttcct cgtacatctc aaggcgcgtg
      361 ccatagccga caagaagaat attgcgggtg aagcggcctt tgcgatagga ggcggagatg
      421 tacgtcgcaa ggacggttcg cacgaaaacc agcaacacga tggcaaaaag gtagtaaagc
      481 ccgatggcga tgcggctgaa tttattatcg tagaagaagt acagcaccgc gaggagaaaa
      541 agaaacgcgt ttgatgaagc gataaaaaga tcgcccgtct ccttccgcca cgattgtccc
      601 aacgattcct tgtacaaccc tcgctggctg atgaagaaga tgttgagtac ccaaaccagc
      661 cagaccagac tcaggaacga aggcaacgcc ggcaacactc cgccccagat cacatagaat
      721 cgcagaattc ctgccagata ccatgccaga atcaccgcaa gcgaatcgcc cagaacccgc
      781 aacgtcaaca accgtttgct cccgtagccc atcatcgata ccccttcttc atgcatccat
      841 cgtaccacag agtggctgga atgagtactc tcttatatcc gtagatggtc gtcataccgg
      901 tcgctgagaa tgcgtttggt atatgcatcc ttctgcttga gaatctttga acctcgagtg
      961 attttgcaca ggctgattcc ataggtcgcg gcgatctccc gctgcggctt cccccggtac
     1021 agatccttca tcagattcca ccggaggatc aaatccttca gctcattggg agtcagaagc
     1081 tcttcgagca attgcctcat gtcatcgtga tcggtcgttt ttgcgaacag cttcagcaga
     1141 tcttcaaaac tttcatccat ggcaacacct cccaaccctt gattctacag agagaaatat
     1201 cgccccgtca agcaaaccgt ttgccatctc cctctttggt gcgtatactc tactcaatgg
     1261 aggaattgat gaagacgaca acactgaccc cccagcggat gaaagctatt cttgcgatgc
     1321 agcagaaaga gattaccgaa tatgagatct acacgcgggt cgccaaccga gtgaaagacg
     1381 aagagaaccg gaagatcctg aaaaagatcg gcgaagagga gcgccggcat gcagccactt
     1441 gggccaaggt gaccggcaag accctcaaac cccagatggg aaaggttcgt ttttattcct
     1501 ttctcgcccg cattctgggg tataccttcg tcctgaagaa gatggagatg ggcgaagaga
     1561 aggcgatcga agggtactcg cacatccttg acgagatgcc tcaggccaaa gagatctatg
     1621 aggatgagca gaagcacgaa gaagcgctga tcgcgatgct cgacgaggag cgcctccaat
     1681 atgtgggttc gatggttctc gggctgaacg acgcccttgt tgaactctcc ggaacactgg
     1741 ccggactgac cttcgcgctc aaaaacaccc gcctcatcgc cttaagcggc ctgatcaccg
     1801 ggatcagcgc gaccttgtcg atggcctcga gcgaatacct ctcgtcgaag aaccgaaacg
     1861 aggagaacgc cggtaaaagc gcgatgtaca cgggagtggc gtacctgatc gccgtagcgg
     1921 cgatggttct cccctacctg cttttatcaa attatttagt ggcattgggc acgatgctgg
     1981 cagtagttct cctgatcatc gtcctcttta cctattatac ctcggtagcg aaggaccttc
     2041 ccttcggacc gcggttcagg gagatggctc tcatctcgat cggggtctcg ggaatctcgt
     2101 tcgtcatcgg aattctggtg aagaactggc tgggaatt
//
LOCUS       DAOPUZ010000223         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_55810_length_2128_cov_2.914616, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000223 DAOPUZ010000000
VERSION     DAOPUZ010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55810_length_2128_cov_2.914616"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PKN79_04350"
     CDS             <1..343
                     /locus_tag="PKN79_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNZ95026.1"
                     /translation="YMPPRNTKEQLARIQRLAQERNLMEISGVDINSSRQSFSCPELL
                     EENARHLIDSAWALVAHEKLSSVDPVYGLFHPENPYRDEALNQRIARYAAIARNSDVH
                     NPYTMIEEVRS"
     gene            340..1491
                     /locus_tag="PKN79_04355"
     CDS             340..1491
                     /locus_tag="PKN79_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ95027.1"
                     /translation="MTVFEDIKREIPPLIRGLSFDGSLGLIIHTLGPGPSALVLPSPS
                     KGDSMKETAKAWQTLFDEYVKKERVYPATVGVGELRYGLGTNYDEAVRGEGVSKLPTL
                     PPALTRSDVVRDKVAVVTGGAQGFGEGMVRSLVDHGAFVYVADLNREGAQALASELNY
                     DAMITVAKAVEVNVTDEASVQAMMDTVVSEVGGVDLFISNAGVLRAGSVKTMSLKDFA
                     FVTDVDYTGFFICTKFASRTMAFQNIPSGAYYTDIVTISSKSGLEGSNKNGAYAGAKF
                     GTIGLTQSFALELVEDNIKVNAVCPGNFLDGPLWSDPKRGLFVQYLETNKVPGAKTIE
                     DVRRAYEAKVPMNRGCTTADVMKAIFYIVEQTYETGQAVPVTGGQVMLN"
     gene            1502..>2128
                     /locus_tag="PKN79_04360"
     CDS             1502..>2128
                     /locus_tag="PKN79_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNZ95028.1"
                     /translation="MKTRAVRLYGINDMRIEEFELPAIKDDEILAKVITNSICMSDHK
                     AAAQGPNHKRIPKDIDKNPVMLGHELCGEIIEVGKKWKDKYHEGMRFTIQPAMNYKGT
                     LDAPGYSFPYIGGDATYVILPHMLMELDCVLPYAGPSYFLGSLAEPLSCVVGTFHAMY
                     HTTAGSYVHHMGIVEGGNAAILAGVGPMGMGAIDYAINNPDRRPGRLVV"
BASE COUNT          484 a          588 c          647 g          409 t
ORIGIN      
        1 ctatatgccg ccgagaaaca cgaaggagca gcttgcgcgc atccaaaggc tggctcagga
       61 gcggaacctg atggagatca gcggggtgga catcaattcc tcccggcaga gcttctcctg
      121 tcccgagctc ttggaggaga acgccaggca cctgattgac agcgcctggg ctctcgtagc
      181 ccatgagaag ctctcatcgg tcgatccggt ctacgggctc ttccaccccg agaatcctta
      241 ccgggatgaa gcgctgaatc agcggatcgc ccgctatgcg gcgatcgcca gaaacagcga
      301 tgtccataac ccctatacca tgattgagga ggtcagatca tgacagtctt cgaagatatc
      361 aaaagagaga ttcctccttt gatccgcggc ctgagctttg acggctccct cggcctgatc
      421 atccacacgc tggggccggg cccaagcgcc cttgtgctgc cttccccttc caaaggggac
      481 tcgatgaagg aaaccgccaa agcatggcag accctcttcg acgagtacgt gaagaaggag
      541 cgggtgtatc cggcgacggt tggcgtcgga gagctcagat acgggctggg gacgaactac
      601 gatgaagcgg tccggggtga aggggtgagc aagctgccga cccttccgcc tgcacttacc
      661 cgatcagacg tggtccgcga caaggtcgct gtcgtcaccg gcggcgctca ggggttcggc
      721 gaggggatgg tccgctcgct cgtcgatcat ggggcgttcg tctatgtcgc cgacctgaat
      781 cgggaagggg cgcaagcgct cgcttccgaa ctgaactacg atgcgatgat caccgtggcg
      841 aaggcggtcg aagtgaacgt gacggatgaa gcttccgttc aggcaatgat ggacacggtc
      901 gtctccgagg tcggaggcgt cgatctcttc atctccaacg cgggcgtcct gagagccggt
      961 tcggtgaaga cgatgagcct gaaggatttt gccttcgtca ccgatgtgga ctacaccgga
     1021 ttcttcatct gcaccaagtt cgcttcccgg acgatggcgt tccagaatat cccctcggga
     1081 gcttattaca ccgacatcgt gacgatcagc agcaaatccg gtttggaggg atccaacaag
     1141 aacggggcgt atgccggtgc gaaattcggg acgatcggac tgacgcagag ctttgctctg
     1201 gagttggtcg aagacaatat caaggtcaat gcagtgtgtc ccggaaactt cctcgacggt
     1261 ccgctttggt cggatccgaa gcggggactg ttcgtccaat acctcgagac gaacaaggtt
     1321 ccgggggcca aaacgattga agatgtacga cgggcctatg aagcaaaggt gccgatgaat
     1381 cggggctgca ccacggccga cgtgatgaag gcgatctttt acatagtcga acagacctat
     1441 gaaaccgggc aggcggtgcc ggtcaccggt ggacaggtca tgttgaatta ggagaattac
     1501 gatgaaaaca cgagcagtaa gactgtatgg catcaacgac atgcggattg aagagtttga
     1561 gcttcctgca atcaaagatg atgaaatcct ggcaaaggtc atcaccaaca gcatctgtat
     1621 gagcgaccat aaagccgctg cccaggggcc gaaccataag cggattccca aagatatcga
     1681 taaaaacccg gtcatgctcg gtcacgagct ctgcggggag atcatcgagg tggggaagaa
     1741 gtggaaagac aagtaccacg agggaatgcg gtttacgatc cagccggcga tgaactacaa
     1801 aggaacgctg gatgcccccg gatactcctt cccgtatatc ggaggggatg ccacgtacgt
     1861 catccttccc cacatgctga tggagctgga ctgcgtcctt ccgtacgccg gtccttccta
     1921 cttcctcgga agcctggccg agccgctgag ctgcgtcgtc ggaacgttcc atgcgatgta
     1981 ccacaccacc gccggttcgt acgtccacca tatggggatc gtcgaaggcg ggaacgcagc
     2041 gatcctggcg ggcgtcggtc cgatggggat gggcgcgatc gactatgcga tcaacaatcc
     2101 cgaccgccgt cccggtcgcc tggtcgtc
//
LOCUS       DAOPUZ010000224         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_55939_length_2124_cov_3.453842, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000224 DAOPUZ010000000
VERSION     DAOPUZ010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55939_length_2124_cov_3.453842"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2124
                     /locus_tag="PKN79_04365"
     CDS             <1..>2124
                     /locus_tag="PKN79_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ95029.1"
                     /translation="DVKKELKTYGKERLIRAYYDMLLIRKFETMLDMIKKEGVYQGIS
                     YNHLGPAHLSAGQESAAVGQAMVLDVDDQIFGSHRSHGEILAKSMSAIAQLSEKELLS
                     IMESYMGGNTFKVVEEHHKGKTVKDLAENFVLYGALAEIYAKKNGFNAGLGGSMHTFF
                     KPFGSMPNNAIVGGSGTIAVGAALYKKINRKKGIVIANIGDGSLARGPVYEALVLASM
                     DQYTTLWDDNPGYPPFLVNVFNNLYAMGGQPSGETMGYGIAARMGAGINQNSMHTERV
                     DGFNPLAVADATARKKALLLEGKGPAMLDTITYRYSGHSPSDAMTYRTKEELDAFRNQ
                     DPIEAYGEYLVKNKIASASELESYDALLEEKMRKTLEITVDPAISTMVDEAFIESVMF
                     SNGSVEKFDTTEPALLQTLEENPRVQQIAKRSRYAYDEEGKMYPAARLYQYRDAVFEA
                     MAHRFAIDPTMVAYGEDHRDWGGAFAAYRGLTELLPPSRFFNSPISEAAIVGSGVGYA
                     LAGGRAVVELMYCDFLGCAGDEVFNQMPKWQAMSGGQLTMPLVLRVSVGNKYGAQHSQ
                     EWTAMVGSVPGLKAMYPATPYDVKGMLNYALRGTDPVVFFESQKLYGIGEQFVKEGVP
                     EGYYEIPEGEPAIRKEGKDITLIALGPSLYTAMKASEKLAELGVDAEVIDLRWINPLK
                     YELLVESVKKTGKVVLVTDSAERGS"
BASE COUNT          482 a          628 c          652 g          362 t
ORIGIN      
        1 cgatgtaaaa aaagagctca aaacctatgg caaggagcgc ctgatccggg cctactatga
       61 tatgctcctg atccgcaagt tcgagacgat gctcgacatg atcaagaagg agggggtgta
      121 tcaggggatc tcctacaacc acctcggtcc cgcccacctc tccgccggcc aggaaagcgc
      181 cgccgtcggg caggcgatgg tccttgatgt ggatgaccag atcttcggct cccaccgcag
      241 ccacggagag atcctcgcca agagcatgag cgcgatcgct caattaagtg aaaaagagct
      301 gctttcgatc atggagagct acatgggcgg aaacaccttc aaggtcgtcg aagagcacca
      361 caagggcaaa accgtaaaag atcttgccga gaactttgtg ctctacggag cgctcgcgga
      421 gatctatgcg aagaagaacg gcttcaacgc cggcctcggc ggctcgatgc acaccttctt
      481 caagcccttc ggctcgatgc cgaacaacgc aatcgtcggc ggttcgggaa cgatcgccgt
      541 gggagcggcg ctctacaaga agatcaaccg caagaaggga atcgtcattg ccaatatcgg
      601 cgacggttcg cttgcccgcg gtccggtcta tgaagcgctc gtcctcgcct cgatggacca
      661 gtatacgacc ctctgggacg acaacccggg gtatccgccg ttcctcgtga acgtcttcaa
      721 caacctctat gcgatgggcg gccagccctc cggcgagacg atgggctacg gaatcgcagc
      781 ccggatgggg gcgggaatca accagaactc gatgcacacc gagcgggtcg acggcttcaa
      841 ccccctcgcc gtggcggatg cgacggcaag gaagaaggcc cttcttctgg aaggaaaagg
      901 cccggcgatg ctcgatacga ttacctaccg ctacagcgga cacagcccca gcgacgcgat
      961 gacctatcgg accaaggaag agctggatgc gttcaggaac caggatccga tcgaagcgta
     1021 cggagagtat ctggtgaaga acaagatcgc atccgcaagc gagcttgaat cgtacgacgc
     1081 ccttctggaa gagaagatgc gcaagaccct cgagatcacc gtcgatcccg cgatcagcac
     1141 gatggtcgat gaagcgttca tcgaatcggt gatgttcagc aacggaagcg tcgagaagtt
     1201 cgacacgacc gagccggctt tgctgcagac cctggaggag aacccccggg ttcagcagat
     1261 cgccaagcgc agccgctatg cgtatgacga ggaaggaaag atgtatccgg cggcccgcct
     1321 ttatcaatac cgcgatgcgg tcttcgaggc gatggcccac cgctttgcga tcgatccgac
     1381 gatggtcgcc tacggcgaag accaccgcga ctggggcggc gcgttcgccg cgtaccgcgg
     1441 tcttaccgag ctgcttcctc cctcccggtt cttcaactcg ccgatcagcg aagcggcgat
     1501 cgtcggaagc ggcgtcggct atgcccttgc cggcggtcgg gcggtcgtcg aactgatgta
     1561 ctgcgacttc ctcggctgcg ccggagacga agtcttcaac cagatgccca agtggcaggc
     1621 gatgagcggt ggccagctga caatgccgct ggttctccgc gtctccgtcg gaaacaagta
     1681 cggcgcccag cactcccagg agtggaccgc gatggtcggc tcggtgcccg gcctcaaggc
     1741 gatgtacccg gcgaccccct acgatgtgaa ggggatgctg aactacgccc ttcggggaac
     1801 cgatccggtt gtcttcttcg agagccagaa gctctacgga atcggggagc agttcgtcaa
     1861 ggaaggggtg cccgagggat actatgagat tcccgaaggg gagccggcga tccgcaagga
     1921 aggaaaggac atcaccctga tcgccttagg cccctccctc tacaccgcga tgaaggcgag
     1981 cgagaagctt gccgagctgg gcgtcgacgc cgaggtgatc gacctcaggt ggatcaaccc
     2041 cctgaagtac gagcttctgg tcgagagcgt gaagaaaacc ggaaaagtgg ttcttgtaac
     2101 cgattcggcc gaacggggca gcta
//
LOCUS       DAOPUZ010000225         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_56220_length_2117_cov_2.770611, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000225 DAOPUZ010000000
VERSION     DAOPUZ010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56220_length_2117_cov_2.770611"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PKN79_04370"
     CDS             complement(<1..534)
                     /locus_tag="PKN79_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNZ95030.1"
                     /translation="MTEREKRYARLLSKMIQVETISEPGIIHEEKFIKSEEVLANLFP
                     KVFSSFERLPIESGLLMRLKGKTEGEGVLLMSHQDVVSAEGEWTYPPFSGEIADGKIW
                     GRGTVDTKGALMCILQAAEELLEEGYIPEVDFYIASSWNEEVTGSGAPNAARYLHDHN
                     VRLQLLKIYRKEGNENED"
     gene            complement(531..1205)
                     /locus_tag="PKN79_04375"
     CDS             complement(531..1205)
                     /locus_tag="PKN79_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95031.1"
                     /translation="MEKSFDYEQYNNSIHRLGRIMNTIGILSLLATPFLFALVLGASL
                     SWSGFAKGLLRVAITYLPITIVEFLIYVPILGPGGSYLSFITGNIINMKLPCAYNARE
                     IAKTQAGTPEDSIITTLAVGVSSLVTMLVIFIGVLLLIPLTPVLNNPTLRPAFDNLLP
                     ALFGALGAQYFFKSWKISAIPLILMIALSIAIPALIAQTTIMMILIGAIAIAITYFFH
                     KKGALQ"
     gene            complement(1216..1950)
                     /locus_tag="PKN79_04380"
     CDS             complement(1216..1950)
                     /locus_tag="PKN79_04380"
                     /inference="COORDINATES: protein motif:HMM:NF027796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HNZ95032.1"
                     /translation="MDYFSQANSAPLYILVGVVLLFVAISCVLFIRKSYLAGLKMGMD
                     RSVLRTTITSSALFTVLPSISILLGVIALSGSLGVPSSWLRLSVVGNLSYEAIAAEAA
                     AQAMGVSLKDAASLTTGHLVTIILVMTTGISWGIIATIFFGKSYSRKSTQLLNKSKKK
                     EGKSFADWAMIAMFIGFCGTFIGSYVAEAVVFKRFIPLLTALVAALVMKGCEYLVKEK
                     HLAWMENFSLSLSMLIAMTAAVLLAL"
BASE COUNT          502 a          557 c          602 g          456 t
ORIGIN      
        1 gtcctcgttt tcattccctt ccttacgata aattttcaga agttggagac gaacgttgtg
       61 gtcatgaaga tatcgtgcag cgttcggagc tcccgatccg gtcacctcct cattccatga
      121 agaggcgatg tagaaatcga cttcggggat gtacccctct tcaagcagct cctcggccgc
      181 ctgaaggatg cacatcagcg ctcccttggt gtcgacggtt ccccggcccc agatcttgcc
      241 atcggcgatt tcgccgctga agggaggata tgtccactcc ccttcggcgg aaaccacgtc
      301 ctggtggctc atgagcagga ccccttcgcc ttcggtcttt cccttcaggc gcatcaaaag
      361 cccgctttcg atcgggagtc tctcaaacga tgagaacacc ttgggaaaga ggtttgcgag
      421 aacctcttca cttttgatga acttctcttc atggatgatt cccggttcac tgattgtctc
      481 cacctgaatc atcttcgaga gcagccgggc gtaccgtttt tcccgttcgg tcattggagg
      541 gctcctttct tatggaagaa ataggtgatg gcgatagcga ttgctccgat aaggatcatc
      601 atgatcgtcg tctgggcgat gagggccgga atcgcgattg aaagggcgat catcaggatc
      661 aaaggaattg cactgatctt ccagctcttg aagaagtact gggcgccaag cgctccgaaa
      721 agcgccggaa gcaggttgtc gaacgcgggg cggagggtcg ggttgttcag gacgggtgtc
      781 aggggaatca acaggaggac tccgatgaag atcaccagca tcgtcacgag ggatgaaaca
      841 ccgaccgcca acgtcgtgat gatcgaatct tccggagttc ccgcctgagt cttggcgatc
      901 tcccgggcgt tgtaggcaca cggcagcttc atgttgatga tattgccggt gatgaaggag
      961 agataggatc ctcccggtcc gaggatcggc acatagatca aaaattcgac gatcgtgatc
     1021 ggcagatagg tgatggcgac tcggagaagg ccctttgcaa atcccgacca ggaaagcgag
     1081 gctccgagca ccaacgcaaa caggaaagga gttgccagca gggagaggat cccgatcgtg
     1141 ttcataatcc gcccgaggcg gtggatcgag ttgttgtact gttcataatc aaacgatttt
     1201 tccattccgt tcctcctaca gggcaagcag aaccgctgcg gtcattgcga tcaacatcga
     1261 aagggagagg gaaaagttct ccatccacgc cagatgcttt tctttcacca gatattcaca
     1321 ccctttcatc accagagccg cgaccaatgc ggtgaggagc ggaatgaagc gcttgaacac
     1381 caccgcttcg gcgacgtagg aaccgatgaa cgttccgcag aagccgatga acatcgcgat
     1441 catcgcccag tcggcgaagc tctttccctc tttctttttc gacttgttga gcaattgggt
     1501 tgatttgcgg gagtaggatt ttccgaagaa gatcgtagcg atgatccccc agctgatccc
     1561 ggtggtcatc accaggatga tcgttaccaa atggccggtg gtcaacgagg cggcgtcttt
     1621 cagggaaacg cccatcgcct gagcggcggc ctcggcggcg atcgcttcat aggagaggtt
     1681 ccccaccacc gaaaggcgaa gccaggaaga gggcaccccc aacgatcctg aaagggcgat
     1741 gacccccagc aggatggaga tcgacggcaa cacggtgaac agggcgcttg atgtgatcgt
     1801 ggttctcagg acgctgcgat ccattcccat cttgaggccg gcaagatagc tttttcggat
     1861 aaagagcacg cagctgattg cgacgaagag cagaaccacc ccgaccagta tgtacagcgg
     1921 tgcgctgttg gcttgactga aataatccat tgtcacctcc attggaggca gtgtatcaaa
     1981 ttatggggat ggaggcgatg aatttttacg ccaatcgcgc caagagaaag gggaaaacga
     2041 ccacattccc gatcgagccg ccaagactcg tgaggaggaa caccactagg atgtggagga
     2101 cgcggttgcg ataccag
//
LOCUS       DAOPUZ010000226         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_56737_length_2104_cov_2.157150, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000226 DAOPUZ010000000
VERSION     DAOPUZ010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56737_length_2104_cov_2.157150"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1260)
                     /locus_tag="PKN79_04385"
     CDS             complement(97..1260)
                     /locus_tag="PKN79_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95033.1"
                     /translation="MIAPFQPHLAALLGEQLASGTFSRVSLFGGDRYTLRMSTAIECA
                     RVLSCAHGGGEHCRCPSCTKFATLSYSDLVIVSQRDHRQRIEAALAAFLHDPNGPWRN
                     RLIQTIRIPLLGFHPILLEEAGSALQNTAARAEGMNNLLYELTAEGVLERNRAEEYVR
                     DLRKTLKDFHSGLPKSTTVSINQVRSIDSWVHQTNISGQLRFVILEGIEQTNVAARNV
                     LLKLLEEPPKGVYFFLISEHPSRIMPTILSRSRQYLFPPLSEENRRSFIASYGLTESN
                     YDSLRSFFLTQSGVNIEESRLLVDRLLASLIEGRALDSRTIASITAEIEKMNSCEYVL
                     SILGEAIAKSVLSRRYVAKAVALIDETYAAGLLYNQNQRFTFEALYYRLLEER"
     gene            complement(1257..1784)
                     /locus_tag="PKN79_04390"
     CDS             complement(1257..1784)
                     /locus_tag="PKN79_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNZ95034.1"
                     /translation="MMQQVSVSLFGFVFNIVDIIVFVLMIVGGISGAFVGFAQTFTRR
                     GGYLIGFMTALLLTRWMQPFLSDTLGLSVFFSSLLSFVLLFIIGYTLIRLFGSMLENA
                     FEAIGLGGVNAFLGFIWGVCEMVIVSAFILFFLESTSLSELTPYLEQSQFVLRFVHRF
                     MPDTVRIIKETASSL"
     gene            complement(1781..>2104)
                     /locus_tag="PKN79_04395"
     CDS             complement(1781..>2104)
                     /locus_tag="PKN79_04395"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ95035.1"
                     /translation="HRADLVVSRSGTGVLEELKLFAKASILLPLGRKTSRGEQEANAR
                     HLEAKGATVVLYEPVDPETFVLTVRSLIDDPLRREALARTMGSLGDGSAARRIGEIIL
                     GWRNA"
BASE COUNT          486 a          607 c          568 g          443 t
ORIGIN      
        1 gcatctccat ctcgcggctt tggcttttaa ggatttcgac gatctgcttt gaatcgagtt
       61 gatcgatctt ttcaatcgcc cggcggacaa agttcttcat cgctcctcca acaatcggta
      121 atacaacgcc tcgaaggtga accgctgatt ctggttgtac agcagcccgg cagcataggt
      181 ttcgtcgatc agcgccaccg cctttgcgac atatcggcgg gaaaggacgc tttttgcgat
      241 cgcctccccc aatatactca aaacatactc gcagctgttc attttctcga tctcggcggt
      301 gatcgaagcg atcgttcggg agtcgagagc ccgtccttcg atcagcgaag caagcagccg
      361 atcgaccagg agcctgctct cttcgatatt cactccgctt tgcgtcagga agaaagatcg
      421 aagggaatcg tagttggatt ccgttaaacc gtaggaagcg atgaagctcc tcctgttctc
      481 ctcgctcagt ggagggaaca ggtattgacg actgcgggag aggatcgtcg gcatgatccg
      541 ggaggggtgt tcgctgatca gaaagaagta tacaccttta ggtggttctt ccaggagctt
      601 caacaggacg tttcgcgcag cgacattggt ttgttcgatc ccctccagga tgacaaaacg
      661 cagttggccg gagatattcg tctgatggac ccagctgtca atcgagcgaa cctgattgat
      721 cgacaccgtg gtcgattttg gcaggccgga atggaaatct ttcagggttt ttcggagatc
      781 acggacgtac tcctccgccc gattccgttc caacactcct tccgccgtca gctcatacag
      841 gagattgttc atcccctccg cccgagccgc ggtgttctgg agcgcggagc ccgcctcctc
      901 aaggaggatc ggatgaaacc ccaaaagcgg gatgcggatc gtttgaatca accgattacg
      961 ccacggtccg ttggggtcgt gcaggaatgc ggcaagcgcc gcttcgatcc tttggcggtg
     1021 gtcccgttga ctgacgatga ccaggtcgga gtatgacaag gtcgcgaact tggtgcagga
     1081 ggggcacctg cagtgttccc ctcctccatg ggcacaggag agaacccggg cgcactcgat
     1141 tgccgtgctc atcctcaggg tgtagcgatc gccgccgaag agtgaaaccc ggctgaacgt
     1201 gccgcttgca agctgctcgc ccaacaaggc tgccaggtgc ggttgaaacg gagcaatcat
     1261 aggcttgagg ccgtttcttt gatgattcgg acggtgtcgg gcatgaagcg gtggacgaag
     1321 cgcaggacaa actggctctg ctccagatag ggggtcaatt cactcaacga ggtcgactca
     1381 aggaaaaaga gaataaacgc ggaaacgatg accatctcac agacccccca gataaacccc
     1441 aggaatgcat tgactccccc aagcccgatc gcctcaaagg cgttttcaag catcgagccg
     1501 aagaggcgga tcaacgtata cccgataatg aagaggagaa cgaacgacag aaggcttgag
     1561 aagaagacac tcagtccaag ggtgtcggag aggaacggtt gcatccagcg agtgaggaga
     1621 agcgcggtca tgaacccgat caaataccct ccccgccggg tgaacgtctg ggcgaagccc
     1681 acgaaggcgc cgctgattcc tccgacgatc atcaatacga acacgatgat atcgacgatg
     1741 ttaaacacaa agccgaagag tgatacgctt acctgctgca tcatgcattc ctccatccca
     1801 gaatgatttc accaatcctt cttgccgccg atccgtcccc caggcttccc attgtccgcg
     1861 ccagcgcctc gcgcctcaga ggatcatcaa tcaacgatcg aaccgtcaaa acaaaggtct
     1921 cgggatcaac cggctcatag agtaccacgg tcgctccctt cgcttccaga tgccgggcat
     1981 tcgcttcctg ctcccctctg cttgtttttc ttccgagcgg aagcaggatg ctcgcctttg
     2041 caaacagctt cagctcctcc aacacccccg tcccgcttcg gctgaccacg agatccgccc
     2101 gatg
//
LOCUS       DAOPUZ010000227         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_58010_length_2072_cov_3.194844, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000227 DAOPUZ010000000
VERSION     DAOPUZ010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58010_length_2072_cov_3.194844"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PKN79_04400"
     CDS             complement(<1..859)
                     /locus_tag="PKN79_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HNZ95036.1"
                     /translation="MNNEHKNDKDWKESLKKRMNIEPGIHTGDKGPQKKYTFSFWYFF
                     IILMAFLAINSFMVSRQPNIVQIDYNQFKQLVENRTIKRVNIETERYVGYSFSLGQLA
                     TDGIIPDGGAQAFATYKVEDPSFVPLLDEMNVEYFATPPPKPGIFSSILSYLLPFIFI
                     VILWRIIFSRMGGGGQGVLSFTQNKAKIVAEGDTGIRFADVAGVDESKYELEEVVDFL
                     KNPDKYTEIGGKIPKGVLLVGPPGTGKTLLAKAVAGEAQVPFFRMSGADFVEMFVGVG
                     AARVRDLFRQ"
     gene            951..1655
                     /locus_tag="PKN79_04405"
     CDS             951..1655
                     /locus_tag="PKN79_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ95037.1"
                     /translation="MSEVVLITGGARRLGRVMAHSLLQKGYRVIVHYHTSEADAFELQ
                     RIHGAKIIQGDLSIPEAVEPLFREALTAYGVIDHVINNASCFERGTIGELTLEAYQRM
                     MNLHTTAPLFLSKALYHHLLERGRTGSIINIVDTRVSKPTASRVGYYLSKSALLEQTK
                     VLAVALGPTLRVNAVSPGAVLSNGDEEYFKTMRRILPLGRTGTAEEVAEAALYLLEAD
                     FVSGIELPVDGGQKLL"
     gene            1741..>2072
                     /locus_tag="PKN79_04410"
     CDS             1741..>2072
                     /locus_tag="PKN79_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95038.1"
                     /translation="MHRPDTRRVGVVAAVLLLAALPLSAIPLDDLLKQAEQNSSSMKN
                     LEITRSNVLLQQAAAKTDDGVKVSVNSGELKYTNPIDTKNNWDVSRQRVSLSGSSVEV
                     TLPNDGKTA"
BASE COUNT          458 a          577 c          581 g          456 t
ORIGIN      
        1 cctgtctgaa gaggtcccgg actcgggcgg ctccgactcc gacaaacatc tcgacgaagt
       61 ccgctccgct cattcggaag aacggcacct gcgcttcacc ggcgaccgcc ttggccagga
      121 gggtcttgcc ggttccgggc ggcccgacga gaagcacgcc cttgggtatc tttccgccga
      181 tctcggtgta tttgtcgggg tttttcaaaa agtcgacgac ttcctccagt tcatacttcg
      241 attcatccac tccggccaca tccgcaaagc ggatcccggt gtccccttcg gccacgatct
      301 tcgccttgtt ctgcgtaaag gagaggactc cctggcctcc gccgcccatc ctcgagaaga
      361 tgatgcgcca gagaatgaca atgaagatga agggcagcag ataggagagg atcgaactga
      421 agatgccggg tttcggcgga ggcgtggcga agtactccac gttcatctca tcgagcaggg
      481 ggacgaacga ggggtcttcc actttgtagg tggcgaacgc ctgcgctcct ccatcgggaa
      541 tgatgccgtc ggtggcgagt tggcccaggc tgaaggagta gccgacatag cgctccgttt
      601 cgatgttcac ccgcttgatc gtccggttct ccaccagctg tttgaactgg ttgtagtcga
      661 tctgcacgat gttgggctgg cggcttacca tgaaggagtt tatcgcaaga aacgccatca
      721 ggatgatgaa gaaataccag aatgaaaaag tatacttctt ttgcggacct ttgtcgccgg
      781 tatggatgcc cggctcaatg ttcatccgct tcttcaagga ttctttccaa tccttgtcgt
      841 ttttgtgttc attgttcatt acattaccct ttgtagtggg acgaatcccc ttgttcaaat
      901 atacttcctc aattgtcaat cgacaacaac cccgttataa tttgcatccg atgagcgaag
      961 ttgtcctgat aaccggggga gcccgacgcc tcgggcgcgt catggcccat agccttctcc
     1021 aaaaaggcta tcgggtcatc gtccattacc acacaagtga agcggacgcc ttcgaattgc
     1081 agagaatcca tggggcaaag atcatccaag gagatctttc gatccccgag gcggtcgagc
     1141 cgcttttccg ggaagccttg accgcatatg gagtaatcga ccatgtgatc aacaacgctt
     1201 cctgttttga gagggggacg atcggggagc tcaccttgga agcgtatcag cggatgatga
     1261 acctccacac aaccgctccg ctgttcttgt ccaaagcgct ctatcatcac ctgcttgagc
     1321 gcggccgaac cggctcgatc atcaacatcg tcgatacgag ggtctccaag cccaccgcct
     1381 cccgagtcgg gtattacctt tcaaaaagtg ccctgctgga gcagacgaag gtgctggccg
     1441 tagctctcgg gccgaccctc cgggtcaatg ccgtcagtcc gggagcggtt ctctccaacg
     1501 gggatgagga gtatttcaaa acgatgcggc gaatccttcc gctggggaga acaggaacgg
     1561 ccgaggaggt cgccgaagcg gctttgtatc ttctcgaagc ggattttgtt tccggtatcg
     1621 aacttccggt ggatgggggt caaaagcttt tgtagtttgt gtgaagaagc ccctcacagg
     1681 ttgccgaaga ggcgaaagat cacgatactt gatagtatgt aacgatagga gtgtagattg
     1741 atgcaccgac cagatacgag acgggtgggg gtagtcgccg cagtactgct tcttgccgcc
     1801 ctcccgcttt ccgcgatacc cctcgatgac ctgctcaaac aagccgagca gaactcctct
     1861 tcgatgaaaa acctcgagat cacccggagc aacgtcctcc ttcaacaggc cgcggcgaag
     1921 accgatgacg gggtgaaggt gagcgtgaac tccggagaat tgaagtatac caacccgatc
     1981 gacacgaaga acaactggga cgtctcccgc cagcgggtct cgctttccgg cagcagcgtg
     2041 gaggtgacgc tgccgaacga cgggaagacg gc
//
LOCUS       DAOPUZ010000228         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_58486_length_2061_cov_3.867896, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000228 DAOPUZ010000000
VERSION     DAOPUZ010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58486_length_2061_cov_3.867896"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /gene="lspA"
                     /locus_tag="PKN79_04415"
     CDS             complement(<1..434)
                     /gene="lspA"
                     /locus_tag="PKN79_04415"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNZ95039.1"
                     /translation="MSKTWRPLILTVFIVLADQLSKAWVVAAIPENTVGYRFFGDFLA
                     IVHVRNTAIAFSMGSALPIWIRLVFFVGLPVVLLVIVLLIYFNEHIPLSMPTRWTLAL
                     FMGGGVGNLIDRIFRSLRVVDFISVKFYGIFGLERWPTFNIA"
     gene            complement(448..1218)
                     /locus_tag="PKN79_04420"
     CDS             complement(448..1218)
                     /locus_tag="PKN79_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNZ95040.1"
                     /translation="MVSTDGMMRSELVAYLDEYLQIESFLSSDSSLNGLVVGGEDKRI
                     GKIGYAVDACQASFDQAAEEKVDFLLVHHGLFWGVPLAVTGSHYTRLATLIKHGIDLY
                     AAHLPLDAHPEIGNNIRIAKALDLEEIEPFAPYKGKTIGYKGRLQEPKTLKEIAGHLG
                     YEKTILLPFGKEKIRTIGIVSGGASRNVSDALNDGLDLYITGEVEHESYHVALEGGIN
                     VLGGGHYVTEVYGVRALAEHLRQRFGLEAVFIANPTGL"
     gene            1244..>2061
                     /locus_tag="PKN79_04425"
     CDS             1244..>2061
                     /locus_tag="PKN79_04425"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ95041.1"
                     /translation="MSERTSQVLPLSFEQASFEFDPALIKRLRNQRLADRIVGQPRAL
                     CSLEMGLSLPKAGYNIFVSGESQSGRHAAVRHAIEQVRDDLSGLRDIVYVCNFTQPDS
                     PHVLTFAPGEGSRFIDSLEQFNHSITLLSEESETFLSNAHSLVDSLIAQFPQKELERY
                     FFGLKGDITRQDAHIRRLGKADEALATRYLGNLVVDHSRSTKRPMIIESHPSMGNLFG
                     TIHAKDKPAHLSYHPGSLLESCGGFIIIDAPELFSKEGLWEALKRYLDATSLAQ"
BASE COUNT          460 a          626 c          545 g          430 t
ORIGIN      
        1 tcggctatgt tgaaggtcgg ccaccgttcc aggccgaaga ttccgtaaaa cttcacgctg
       61 atgaaatcca cgaccctgag ggagcggaag atccggtcga tgaggtttcc gactccaccg
      121 cccatgaaga gggcaagcgt ccatcgggtc ggcatcgaga ggggaatatg ctcattgaag
      181 taaatcagca gaacgatcac cagcaacact accggcagac ctacgaaaaa gaccagtcgg
      241 atccaaatgg gaagcgcaga acccatgctg aaggcgatcg cggtgtttcg gacgtggacg
      301 atcgccaaaa aatccccgaa gaaccggtag ccgacggtat tctccggaat cgcggcgacg
      361 acccatgctt tggaaagctg atccgcaagc acgatgaaca cggtgagaat gagagggcgc
      421 catgtttttg acatacggga aactatacta taagccggtt gggttggcaa taaacaccgc
      481 ttcgaggccg aaacgctgtc gaaggtgctc ggccagagcc ctcactccgt acacttccgt
      541 aacgtaatgt ccgccgccga ggacattgat tcccccttcc agcgccacat ggtagctttc
      601 atgctccacc tcgccggtta tatagagatc caagccatca ttcagtgcat cggagacgtt
      661 tcggctcgcc cctccgctga cgattccaat cgttcggatc ttctccttcc cgaagggcag
      721 aaggatcgtt ttctcatatc cgagatgccc ggcgatctcc ttcagcgtct tgggctcctg
      781 cagccgccct ttatagccga tcgtcttccc tttgtaggga gcgaacggtt cgatctcttc
      841 caagtcgagc gctttggcga tccggatgtt gtttcctatt tccggatgag catccaacgg
      901 caaatgcgcg gcatacagat cgatgccgtg tttgatgagg gtcgccaatc tcgtatagtg
      961 ggaaccggtt accgcgaggg ggactcccca gaacaggccg tggtggacga gcaggaaatc
     1021 caccttctcc tctgccgcct gatcaaaaga ggcctggcag gcatccaccg catacccgat
     1081 cttcccgatc cgtttatctt cgccgccaac caccagcccg ttgagcgagc tgtccgatga
     1141 gagaaagctc tcgatctgta gatactcatc caaataggcg accagttcgc ttcgcatcat
     1201 tccatcagtt gacaccatcg gtctctcctc ctatagttcg ctcatgagcg agcgcacctc
     1261 tcaagtattg ccgctttcct ttgaacaggc aagcttcgag ttcgatcccg ccctcatcaa
     1321 gcggctgcgg aatcaacggt tggccgaccg gatcgtcggt cagccaaggg ctttgtgttc
     1381 cttggagatg ggtctttccc tgccgaaagc tggatacaac atcttcgtca gcggggagag
     1441 ccagagcggc cgccacgccg ccgtccgtca tgcgatcgaa caggttcggg acgacctctc
     1501 cggcctcagg gacatcgtgt atgtctgcaa cttcacccaa ccggacagcc cgcacgtgct
     1561 cacgttcgcc cccggcgagg gctcgaggtt catcgattcg ctggaacagt tcaaccactc
     1621 gatcaccctc ctttccgagg agagcgaaac gtttctgagc aacgcccata gccttgtcga
     1681 ttccctgatc gcccaattcc cccaaaagga gcttgaacgc tatttcttcg gtctgaaagg
     1741 ggacatcacc cgccaggatg cccacatccg gcggctgggg aaagcggatg aagcgctggc
     1801 cacccgctat ctgggcaacc tcgtcgtcga ccacagccgc tcgacgaagc ggccgatgat
     1861 cattgaaagc cacccttcga tgggaaacct tttcgggacg attcatgcaa aggacaagcc
     1921 cgcccacctt tcctaccacc ccggttcgct cctggaaagc tgcggagggt tcatcatcat
     1981 cgatgcgccc gagctcttca gcaaagaagg actttgggag gcgttgaagc ggtatctcga
     2041 tgccactagc ttggcgcaaa a
//
LOCUS       DAOPUZ010000229         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_58540_length_2060_cov_2.909726, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000229 DAOPUZ010000000
VERSION     DAOPUZ010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58540_length_2060_cov_2.909726"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1667)
                     /locus_tag="PKN79_04430"
     CDS             complement(138..1667)
                     /locus_tag="PKN79_04430"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNZ95042.1"
                     /translation="MVVEAKAVNVETLVEVKNCTMVFPGVKALDNVDFTLLAGECHGL
                     VGENGAGKSTLSKCIIGDNRMTEGELYIQGNKIHIPSFNVRKSQEMGIAIVHQEFQLM
                     GDLNGLENIFIGRYTKRGPLIDWKELQKEGEALLEYLQCDVDLKVKVKHLRTAEKQII
                     QLAKAMLDNPKVIIFDELTSVLQEDGIENIFRIIRLLKEKGIGIIYISHRLDEIFECC
                     DRYTVLCDGKWINSGYVKDIDKPELIKMIIGRELKNVYPPKNEEFGETLLEVKNLTAH
                     DGSFRDVSLSVRAGEVVGISGLLGAGKTELIHAIYGNYKTTSGDVFVRGKQVEIKSPQ
                     HAIKLGLGVIPDERRLLGLNMLFNIQDNTTLPSMFMFKKWKIFQNHRAEEKAAFDIND
                     KLKLRYVSLSQLISKLSGGNQQKVVIAKWMLRNCDLFLLDEPTRGIDIGAKFEIYSLI
                     NELAKAGKGIVLVSPELEEIIGLSNKVYVMYEGKVMDTVEGDDINQEVIIQDLLGVKE
                     A"
     gene            complement(1658..>2060)
                     /locus_tag="PKN79_04435"
     CDS             complement(1658..>2060)
                     /locus_tag="PKN79_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95043.1"
                     /translation="HPKIFEKLTNLHKACLADPKLRHWAPHFAKLKNAERRNMVIYEM
                     IKASLRYEDEKDPKKKDRILDEILAYNEEDFAIAKEMFFDINPVGETGVKSSMFPYHE
                     IKRLIHNIRNPENPDNDIICSGIEALGWLWL"
BASE COUNT          434 a          584 c          477 g          565 t
ORIGIN      
        1 ggtggttctg aactttggaa gggcgatgct gcagatgata aacaaggcga tgaacaggaa
       61 gagaatcatc cattctttaa aaaaggcctt gtaatccttt ttaaacaacc tttcatcgcc
      121 gccaagtact tttgtgctca tgcttctttc actcctaata gatcttggat gatcacttcc
      181 tgattgatat cgtcgccttc aacagtatcc atcactttcc cctcatacat cacatacacc
      241 ttgttggaaa gtccgatgat ttcttccagt tccggtgaaa ccaatacgat tcctttcccc
      301 gctttcgcca gctcgttgat caatgaatag atttcaaact tcgctccgat atcgatgccc
      361 cgggtcggtt catccagcag gaacaggtcg cagttgcgca gcatccattt ggcgatgact
      421 actttctgct ggtttccgcc cgaaagcttg ctgatcaatt ggctgaggct cacatagcgg
      481 agcttcaact tgtcgttgat gtcaaaagcc gctttctcct ccgcccgatg gttctggaag
      541 atcttccact tcttgaacat gaacatcgac ggaagcgtcg tgttgtcctg aatattgaac
      601 agcatgttca aaccgagcag ccgccgctca tcgggaatca ctccgagccc cagtttgatc
      661 gcatgctgcg gagacttgat ctccacctgt ttcccccgta cgaagacgtc gccggatgtt
      721 gttttatagt ttccgtagat cgcatggatg agctcggtct tgccggcgcc cagcaatccg
      781 gaaatcccga cgacctctcc tgcccgaacg gacagcgaga catcgcggaa cgatccgtcg
      841 tgcgcggtga ggtttttcac ttccagaagt gtttcaccga actcctcatt ctttggcgga
      901 tatacattct tcagctcgcg gccgatgatc attttgatca attcgggttt gtcgatgtct
      961 ttcacatatc cgctgttgat ccatttcccg tcacagagaa cggtgtaccg gtcgcagcac
     1021 tcgaagatct catcaaggcg gtgggagatg tagatgattc ctatcccctt ctccttcaaa
     1081 agacggatga tccggaagat gttctcgatc ccgtcctcct gcagcaccga agtcagctcg
     1141 tcgaagatga tcaccttcgg attgtccagc atcgccttgg ccagctggat gatctgcttt
     1201 tcggctgtcc ggaggtgttt gaccttcacc ttcagatcca cgtcgcactg aagatattcc
     1261 aagagcgcct cgccctcctt ctgcagctcc ttccagtcga tgagcggacc tcgtttggta
     1321 tatcttccga tgaagatatt ctccagaccg ttgaggtccc ccatcagctg aaactcttgg
     1381 tggacgatcg cgatccccat ctcctgggac tttcgaacgt tgaaggaggg aatatggatt
     1441 ttattccctt ggatgtacaa ctctccctcc gtcattcgat tgtcaccgat gatgcacttc
     1501 gaaagcgtcg attttccggc tccgttctca ccgacaagcc cgtggcattc ccctgcgagc
     1561 agcgtgaagt ccacattgtc caacgccttg actccgggga acaccatcgt acagtttttt
     1621 acttctacca atgtctccac attgaccgcc tttgcctcta caaccatagc caaccgagag
     1681 cctcgattcc cgaacagatg atgtcgttgt cggggttctc cgggttcctg atgttgtgaa
     1741 tcaaccgctt gatctcgtga tacgggaaca ttgagctctt gaccccggtc tctccgaccg
     1801 gattgatgtc gaagaacatc tccttggcga tggcgaaatc ctcctcgtta taggcgagaa
     1861 tctcatccag gatccgatct tttttcttcg gatccttctc gtcctcatac ctgagcgaag
     1921 ccttgatcat ctcgtagatc accatgttgc gccgctcggc gttcttcagc ttcgcaaagt
     1981 gcggcgccca gtggcggagc ttcggatcgg ccagacacgc cttgtggagg ttggtgagct
     2041 tctcaaagat cttgggatga
//
LOCUS       DAOPUZ010000230         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_58559_length_2059_cov_3.617265, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000230 DAOPUZ010000000
VERSION     DAOPUZ010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58559_length_2059_cov_3.617265"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PKN79_04440"
     CDS             <1..675
                     /locus_tag="PKN79_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ95044.1"
                     /translation="SDYCNYPAEAEALPSVGTLYNPSLEMILALEPTHVISSAFVPDE
                     LLFSIEQANINVLSISAQETFEGTYDLIRAVGDVIHRQSQAEQLIGEMKAQVKAIEEK
                     AAPLSKVTVYMALDFGSFDSAATGDTFLHEMIEKAGGTNVAADGRYWTYSKELLVEKE
                     PQLILLSPRWGESEDATIREFKTTKPYSDLKATVKGFDADVVLRQGPRSAQALQILFD
                     LIDGER"
     gene            677..1657
                     /locus_tag="PKN79_04445"
     CDS             677..1657
                     /locus_tag="PKN79_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNZ95045.1"
                     /translation="MRRHLGWIILVATLTLALVSLTIGSSRIGLSETIGVFTGRNTSS
                     AQRYIILNLRLVRIIASFAAGTILALGGVSYQAVLRNPMADPFILGVSSGASFGVALA
                     IFFGVATRFGLPLSALLGATGTTLFILALASRKRSSNTVLLLSGVAANYILSAAMTLL
                     LFLNREQYQRILAWSLGTFSTATRNQVVIASLVSVLLLLFLIGNHRNLDLLLLDEGSA
                     RSSGLAVESVRYRLLLISSFATALCVSFFGVIGFIGLMAPHITRLILGAKHRRLIFPT
                     ALLGGFLLLLSDTLARVLLPSGELPVGIITSLLGVPLFLFLILKGRYRFG"
     gene            1650..>2059
                     /locus_tag="PKN79_04450"
     CDS             1650..>2059
                     /locus_tag="PKN79_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ95046.1"
                     /translation="MADAIVIDALTGGYENNTLFRKISLTFPKGAFIALAGPNGSGKS
                     SLLKFFYKQLKPEEGTIWVEKADLATLKQEVLAQNIGFVPQNGRLDFSFTVEETVAMG
                     RYANPQKDDAKRIYQAMETCSILNLRNTSVTRISG"
BASE COUNT          398 a          623 c          558 g          480 t
ORIGIN      
        1 agcgactact gcaactatcc ggcggaagcc gaagcgctcc caagtgtcgg gacgctctac
       61 aatccttccc ttgagatgat tcttgctctt gagccgaccc atgtgatctc ctcggcgttc
      121 gttcccgatg aactgctctt ttccatcgag caggcgaata tcaacgtgct ttcgatcagt
      181 gctcaggaga cgttcgaagg aacctatgat ctgatccggg ccgttggcga tgtcattcac
      241 aggcaaagcc aagcggagca gctcattgga gaaatgaagg cccaagtcaa ggcgatcgaa
      301 gagaaggccg ccccgctatc gaaggtcacg gtctacatgg ctctggattt cggctccttc
      361 gattcggcgg cgaccggcga tacgttcctc catgagatga tcgaaaaagc cggcgggacg
      421 aacgtcgccg cggacggccg gtactggact tactccaagg agctactggt cgaaaaggag
      481 ccccagctga tccttctttc gcctcggtgg ggagaaagcg aagatgctac gatccgtgag
      541 ttcaaaacaa ccaagccgta cagcgacctg aaggcgacgg tcaaaggctt cgatgcggac
      601 gtcgttctgc gccaaggtcc ccgaagcgcg caggcgcttc agatcctctt cgacctgatc
      661 gacggggaac gataggatga ggcggcacct cggatggatc atcctggtgg caacgctgac
      721 gctggcgctc gtctcgctta cgatcggctc ctccaggatt ggcttgtccg agacgatcgg
      781 agtgttcacc ggccggaaca cctcttccgc ccaacggtat atcatcctca acctgcgttt
      841 ggtgaggatc atcgcctcct tcgcggccgg gacgatcctc gccctcggag gcgtctccta
      901 ccaggcggtt cttcgcaatc cgatggccga tcccttcatt ctgggggtca gcagcggagc
      961 ctccttcggg gtcgcgctcg cgattttttt cggcgttgct acccggttcg gtcttcccct
     1021 ctccgccctg ctcggggcga cggggacgac cctcttcatc cttgccctgg caagccgaaa
     1081 acggtcaagc aacaccgtcc ttctgctcag cggagtggct gcgaactata ttctttcagc
     1141 agcgatgacg ctgctcctct ttctgaaccg ggagcaatat cagcggattc tcgcctggtc
     1201 gctgggaacc ttttccaccg caacccggaa tcaggtcgtc atcgcttccc tcgtctcggt
     1261 tcttcttctc ctcttcctca ttggaaacca tcggaatctt gatctgcttc tgcttgatga
     1321 aggttcggcc cgctccagcg ggctggcggt ggaaagcgtc cgctaccgcc tgcttctgat
     1381 ctcttcgttt gccaccgcct tatgcgtttc tttcttcggc gtcatcggtt tcatcggatt
     1441 gatggctcct catatcaccc gcctgatcct cggggcgaaa caccgacggc tgattttccc
     1501 caccgccctt ttgggcgggt tcctcctcct gctcagcgat actctggccc gggttctcct
     1561 gccctcgggg gagctgccgg ttggaatcat cacgagcctt ctcggcgtgc ccctcttctt
     1621 gttcctgatt ctcaaaggga ggtatcggtt tggctgatgc gatcgtcatt gacgccctga
     1681 ccggcgggta tgagaacaat acgttgtttc ggaagatctc cctcaccttc cccaaggggg
     1741 cgttcatcgc cttggccggc cccaacggca gcggcaagtc aagcttgttg aagtttttct
     1801 ataaacaact caaaccggag gaaggaacga tctgggttga aaaagcggat ctcgcaaccc
     1861 tcaaacagga agtcctggct caaaacatcg gattcgttcc tcaaaacgga aggttggatt
     1921 tttcttttac cgtggaagag accgttgcga tgggccgata tgcgaatccc caaaaggatg
     1981 atgcgaagcg gatttaccag gcgatggaaa catgttccat tctcaacctc cgaaacactt
     2041 cggtcacccg aatctccgg
//
LOCUS       DAOPUZ010000231         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_58563_length_2059_cov_3.085329, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000231 DAOPUZ010000000
VERSION     DAOPUZ010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58563_length_2059_cov_3.085329"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKN79_04455"
     CDS             complement(<1..548)
                     /locus_tag="PKN79_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ95047.1"
                     /translation="MKSLIAFWNILKKDVKTYYLKPPNISWGIIFPLSWTLMQLIRSP
                     DGSILTLLPGLIALSVLFGTTSMLAVTITFERSSRSFDRLLLAPISLNTLVLAKISGT
                     ILFGIFNAFVPVVFASFFIDLSAVTWTTVLLAVLMIAITSALTGLMIAVSAKQVFEAQ
                     TFSNFFRFPMLFLCGLFIPISSL"
     gene            complement(545..1507)
                     /locus_tag="PKN79_04460"
     CDS             complement(545..1507)
                     /locus_tag="PKN79_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ95048.1"
                     /translation="MIKASHLTKRYGDITAVDDISFDIRKGELFGFLGPNGAGKTSTI
                     NMIIGLSRPSAGTITVDGIDVASRTKKAQAIMGIVPDESNLYNELDGFENLCFCASLY
                     GMRKKEREEKAHYLLEQFRLENAGTRPFKAYSKGMRRKLTIAAALIHSPGILFLDEPT
                     TGIDVESSRHIREMIVKLKKKGTTIFLTTHYIEEAERICDRIAFICEGRIVASGTVPQ
                     LMDRVTHGHAIQFVTDKTSTSSIGDLQTRFEGSVVKTRSGNSLVMRSDRRIPLYPVME
                     FFHGKGIEVYEARELHPSLEDVFVELTGIESTVLRKEKEKGGFS"
     gene            complement(1509..1898)
                     /locus_tag="PKN79_04465"
     CDS             complement(1509..1898)
                     /locus_tag="PKN79_04465"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNZ95049.1"
                     /translation="MNKILKAIADETRMRILQLLLKHDYCVRALATELNLTEATISQH
                     LKVLREAGLLVGEKRGYFMHYAVERPVLVELSKSIDALAAIEHAPCKPETREDCLAED
                     GHDCAKKGTCSDEIRELCHGSDRNMER"
BASE COUNT          466 a          535 c          562 g          496 t
ORIGIN      
        1 aggctggaaa tcggtatgaa caggccgcag agaaagagca tcggaaagcg gaagaagttg
       61 gaaaacgtct gagcctcgaa gacctgcttg gccgataccg cgatcatgag cccggtcaat
      121 gcggacgtga tcgcgatcat caggaccgcg aggagaaccg ttgtccaagt caccgcactc
      181 agatcgatga agaagcttgc gaaaacgacc gggacgaagg cattgaagat gccgaagagg
      241 atcgttccgg aaatcttggc aagcaccagc gtgttcaggc tgatcggtgc caggagcaat
      301 cggtcgaagg atctggaact ccgttcaaag gtgatcgtga cggcgagcat cgaggtcgtg
      361 ccgaagagaa cgctcagcgc gatcagaccg ggaagtaggg tcagtatgct cccgtcagga
      421 gagcggatga gctgcatcag ggtccatgaa agggggaaga tgatgcccca gctgatgttc
      481 ggtggtttca ggtagtaggt ctttacatcc tttttcagga tgttccagaa cgcaatcagg
      541 cttttcaaga aaatcctcct ttttcctttt cctttctcag cacggttgat tcgatgccgg
      601 tcagctcgac aaagacatct tccagggaag gatggagttc ccgcgcttca tatacttcga
      661 tccctttgcc atgaaaaaac tccatgacgg ggtacagggg aatcctgcga tcggatcgca
      721 tgacaagcga attgccggat cgggtcttta cgacgctacc ttcaaaacgg gtctgcaggt
      781 caccgataga tgatgtactg gtcttatcag tgacaaattg gattgcatga ccatgggtga
      841 cacggtccat cagctgcgga acggttccgg atgcgacgat ccgcccctcg cagatgaatg
      901 cgatccggtc gcagatccgc tctgcctcct cgatatagtg ggtggtgagg aagattgtgg
      961 ttcccttctt ctttagtttc acaatcatct cgcggatatg tctggagctc tccacgtcga
     1021 ttcccgtcgt tggctcgtcc agaaacaaga tgccggggga gtggatgagg gcggccgcga
     1081 tggtcagctt gcgccgcatc cccttggaat aggctttgaa gggtcgcgtc ccggcattct
     1141 ccaacctgaa ctgttctaaa agataatgag ctttttcctc tctttccttc ttgcgcatcc
     1201 catagaggga agcgcagaag cagaggttct caaacccgtc cagttcattg tagagattgc
     1261 tttcatcggg aacgatcccc atgatcgcct gagctttttt cgtccggctg gctacatcga
     1321 tgccgtcgac ggtgattgtt ccggcactcg gtcgggacag gccgatgatc atgttgattg
     1381 tagatgtctt tccggcgccg ttcggcccca ggaagccgaa cagctctcct ttccgtatgt
     1441 cgaaggagat gtcatccact gcggtgatat cgccataccg cttcgtcagg tgcgaggcct
     1501 tgatcatctc atcgttccat attccggtct gaaccgtgac agagttccct gatctcgtcg
     1561 ctgcaggtgc ctttcttggc gcaatcgtgc ccgtcctcgg cgaggcagtc ctcccgggtc
     1621 tccggcttgc atggagcatg ctcgatggcc gccagagcgt cgatgctctt cgagagttcc
     1681 actaagaccg gtcgttcaac ggcataatgc atgaaatatc cccgtttctc tccgacaaga
     1741 aggcctgcct ccctcagcac cttcagatgc tgggagatcg ttgcttcggt caggttcagc
     1801 tcggtcgcaa gcgcccggac gcaatagtcg tgcttcagta gcagctgcaa gatgcgcatt
     1861 ctcgtctcat cggcgatcgc tttcagaatc ttattcatat ccatggtgtt cctccaagaa
     1921 tataattagt cagtcagcta atcaaataat atcgaagaat cctgattagg taaacaccta
     1981 attataaaag atcatcgttt tccgtgctca aggatgtgta atcccgatat tcatcgacaa
     2041 gtctgccagg gaagagatg
//
LOCUS       DAOPUZ010000232         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_58617_length_2058_cov_3.082376, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000232 DAOPUZ010000000
VERSION     DAOPUZ010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58617_length_2058_cov_3.082376"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKN79_04470"
     CDS             <1..329
                     /locus_tag="PKN79_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95050.1"
                     /translation="HYAHLFIVDNPVYEPFHPEPFDGMFTAHSHWGTVFLVKEGEVLA
                     CSTHARQPGTLLRDINGFVHHESSGITSTARVDANHFIFFHPYEPYALIAEKEAAVAR
                     LLVEVR"
     gene            402..1448
                     /gene="ispG"
                     /locus_tag="PKN79_04475"
                     /gene_synonym="gcpE"
     CDS             402..1448
                     /gene="ispG"
                     /locus_tag="PKN79_04475"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNZ95051.1"
                     /translation="MNDVHLVHIGNLTIGKGKPILVQTMYDDTLPVDMKRAEALLIRI
                     GKLNTIGCDIIRFSYPSKDDHEVFKYLAKASPIPIVADIHFDHTLALDAIEAGAHKIR
                     INPGNIGARWKVDEVVRAAKDRDIAIRIGLNSGSLPRREAASPAHLMKDTALEYLDWF
                     ERLGFTNTVVSLKGSDTDLTIEANRLFAKESSYPIHLGVTEAGSVISAVTRSTWALGT
                     LLSEGIGNTLRISITGEIEMEIQAGVELLRTLGLKKKGIRVVSCPRCGRHTFDTIGFL
                     SSVEEELLAIEKDLTVAIMGCQVNGPGEAKEADVAITGIGNAIFLYEKGVLSRKVTAQ
                     DAKEALLEAIRNAE"
     gene            1438..>2058
                     /locus_tag="PKN79_04480"
     CDS             1438..>2058
                     /locus_tag="PKN79_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNZ95052.1"
                     /translation="MQNKLVIKGAREHNLKNIDLTLTKNQLIIISGISGSGKSSLAFD
                     TIFAEGQRRYVESLSSYARMFLDKLDKPDVDYMEGLSPAIAIEQRSTQRNPRSTVGTV
                     TEIYDYFRLLWARVGTPHCHVCHRKISEMSVDQIIDSIFKAKDQSRIIVSSPVAIGKK
                     GEFRKVFEDAKAQGYHRLVVDGQQIDLEDGIPALDKQIKHSIDIIVD"
BASE COUNT          508 a          564 c          574 g          412 t
ORIGIN      
        1 accattacgc ccatctcttc atagtggaca atccggtgta tgaaccgttc catccggagc
       61 ccttcgacgg gatgttcacc gcgcacagtc actggggcac ggtgtttctt gtcaaggaag
      121 gagaggttct cgcctgttcg actcatgccc ggcagccggg caccctcctg agagacatca
      181 acgggttcgt acaccatgaa tcgagcggga tcacctcgac ggctcgggtg gatgcgaatc
      241 acttcatctt ctttcacccc tatgagccct acgccctcat cgcggaaaaa gaggccgccg
      301 tcgcccgact gcttgtcgaa gtccgataag ctacacaagc gtgtagtatc actattcaaa
      361 acgccaatcc tttagtacac tcaactcaag gagaaaaaac gatgaatgac gttcatcttg
      421 tccatatagg aaatctgacg atcgggaagg gcaagccaat cctcgtccaa acgatgtatg
      481 atgatacgtt gccggttgat atgaagaggg cggaggcgct gctcattcga atcggcaaac
      541 tgaatacgat cggctgcgac atcatccgtt tcagctaccc ctcgaaggat gatcatgagg
      601 tgttcaaata ccttgcgaaa gcctccccga tcccgatcgt cgcggatatc cactttgatc
      661 atactctggc gttggatgca atcgaagcag gagcgcacaa gattcggatc aacccgggaa
      721 acatcggagc gaggtggaaa gtcgacgagg tggtccgggc ggcgaaagac cgcgacatcg
      781 caatccggat cggattgaac agcggttccc tgccgcgcag ggaagcggct tcaccggccc
      841 atctgatgaa ggataccgcc ttggaatatc tggattggtt cgagcgtctc ggcttcacca
      901 acacggtggt ctccctgaag ggaagcgata ccgatttgac gatcgaggcc aatcgacttt
      961 ttgcaaaaga aagctcctat ccgatccacc tcggggttac cgaagccgga tcggtcatct
     1021 cggcggtaac ccgttcgacc tgggcgctcg gaaccctgct gagcgaaggg atcggaaaca
     1081 cgctcaggat ctccatcacc ggagaaatcg aaatggaaat ccaggcaggt gttgagcttt
     1141 taaggacgct cggtctgaaa aagaagggaa tcagagtcgt ttcctgtccc cgctgcggcc
     1201 gtcacacctt cgatacgata ggcttccttt catcggtgga ggaggagctg cttgcgatcg
     1261 aaaaggatct gaccgtggca atcatgggtt gtcaggtgaa cggtccgggg gaggcgaagg
     1321 aggccgatgt ggcgatcacc ggaatcggca atgcgatctt tctctatgaa aaaggcgtgc
     1381 tcagccggaa ggtgacggcc caagacgcga aagaggcgct tctggaagcg attcgaaatg
     1441 cagaataagc tggtcatcaa aggtgctcgg gaacacaact taaagaatat cgacctgacc
     1501 cttacgaaaa atcagctgat catcatcagc ggaatctccg gaagcgggaa atcctccctg
     1561 gcgttcgaca cgatctttgc cgaagggcag aggcggtacg tcgaatcgct ctcctcctat
     1621 gcccggatgt tcctcgacaa gttggacaag ccggatgtcg actacatgga aggcctctcc
     1681 ccggcgatcg cgatcgaaca gcgctcgacc cagcgcaacc cccgttcgac cgtcggtacg
     1741 gtgacggaga tctacgacta cttccgcctt ctttgggccc gggtcggaac cccgcattgc
     1801 catgtgtgtc accgaaagat cagcgagatg agcgtcgacc agatcatcga ttcaatcttc
     1861 aaggcgaaag atcaaagccg gatcatcgtc agcagccccg tggcgatcgg gaagaaaggg
     1921 gagttcagga aagtcttcga ggatgcgaag gcccaagggt atcatcgcct cgtcgtcgac
     1981 ggacagcaga tcgatcttga agacggcatt cccgccctcg acaagcagat caagcattcg
     2041 atcgacatca tcgtcgac
//
LOCUS       DAOPUZ010000233         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_58749_length_2055_cov_2.394500, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000233 DAOPUZ010000000
VERSION     DAOPUZ010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58749_length_2055_cov_2.394500"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKN79_04485"
     CDS             <1..329
                     /locus_tag="PKN79_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNZ95053.1"
                     /translation="NCDPTYITYPIPGNDDAIRAISLFVEIIANAVIDADNEAGIQII
                     ESLGDDDEPIEEPEVSKKDDDDDDEAIVYSTDDDADYSKFEDEEEEEDDRTDEDDDED
                     ETLYND"
     gene            342..1193
                     /gene="tsf"
                     /locus_tag="PKN79_04490"
     CDS             342..1193
                     /gene="tsf"
                     /locus_tag="PKN79_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNZ95054.1"
                     /translation="MVITADLVKKLRDATGAGMMDCKKALTEANGDFAEAEKILKKMG
                     LAAVAKRQDRATDNGRVFVKVAKNKAILINLTCETDFVATNKEFIALGEKICDVALEK
                     GYTEINDELNGMVNDLISIIKENMSLANLTVYDVGADELASTYTHGEGSLAVLVKIKA
                     GDPAVFEKEEVKEFANDLALHVAAFTPSYLSREKVDEKYIAEQVEIFTAQAAKLDKPA
                     KVVEGIVKGKLNKHLSEICFLDQAFVKDDTVTVAQKAKEIAKAVGTTIEIVDYAYLKA
                     GWASCAI"
     gene            1251..1805
                     /gene="frr"
                     /locus_tag="PKN79_04495"
     CDS             1251..1805
                     /gene="frr"
                     /locus_tag="PKN79_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNZ95055.1"
                     /translation="MHNVLESCETKMKKSVESLQREFATLRTGRASASLLDRIRVDYY
                     GAETPLNQVSTISTPEARTIVIQPWDKSLLGVIEKAILKSDLDLPPNNDGKLIRLNFP
                     PLNEERRKALVKTAKSIAEQSRVAIRNIRREAIDDLKKLEKSSEISEDEQRDGETRVQ
                     KLTDSYIEQINALSDEKEKEIMEI"
     gene            1809..>2055
                     /locus_tag="PKN79_04500"
     CDS             1809..>2055
                     /locus_tag="PKN79_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HNZ95056.1"
                     /translation="MSDDRLGLKHLGIIMDGNGRWAKKRNLPRTAGHLEGLKALKRVI
                     IEAANQQIEWVTFYAFSTENWKRTEEEVSYLMRLFTSK"
BASE COUNT          589 a          509 c          562 g          395 t
ORIGIN      
        1 cgaactgcga tcccacctat atcacctacc cgattccggg aaacgatgac gcgatccggg
       61 cgatcagcct gttcgtcgaa atcatcgcca acgccgtcat cgacgcggac aacgaagcgg
      121 gaatccaaat catcgaaagc ctcggtgatg atgacgagcc gatcgaagag ccggaagtct
      181 ccaaaaaaga cgatgacgat gacgacgaag ccattgtcta ctccactgat gacgatgccg
      241 attattccaa gtttgaggac gaggaagaag aggaagatga tcgcaccgat gaagacgatg
      301 atgaggacga gacgctctat aacgactaag gaggagcaca tatggtaatt accgctgatt
      361 tggtaaagaa actccgggat gccaccggtg ccggaatgat ggattgcaag aaagcgctca
      421 ccgaagcaaa cggcgacttt gccgaggctg agaaaatttt aaagaaaatg ggccttgccg
      481 ccgttgcgaa gcgccaagac cgggcaaccg ataatggccg ggtgttcgtc aaggtcgcaa
      541 agaacaaagc cattctcatc aacctcacct gcgagacgga cttcgttgca acgaacaagg
      601 agttcatcgc cctcggtgag aagatctgcg acgtagcgct tgagaagggc tacacggaga
      661 taaacgatga gcttaacgga atggtgaacg acctgatctc catcatcaag gagaacatga
      721 gtctcgccaa cctcaccgta tacgatgtcg gtgctgatga actggcctcc acctacaccc
      781 atggggaagg ctccctcgct gtattggtga agatcaaagc gggcgatccc gccgtcttcg
      841 aaaaagaaga agttaaggag tttgccaacg atctcgccct gcacgtagct gcgttcaccc
      901 cctcctacct cagtcgggag aaggtggatg agaagtacat cgccgaacag gttgagatct
      961 tcactgctca ggctgcgaag ctcgacaaac cggcaaaggt cgttgaggga atcgtcaaag
     1021 gcaagctgaa caaacactta agcgaaattt gcttcctgga tcaggccttt gtgaaggatg
     1081 atacggttac cgttgcccag aaagccaagg agatcgccaa agcggtcggc acgacgattg
     1141 agatcgtcga ctacgcctac ctcaaagccg gttgggcttc ctgcgccatc taagacaaga
     1201 atgaagaacc accactcact tccgtgagtg gtggctttat tggaggagtc atgcacaacg
     1261 tattggaaag ctgcgaaaca aaaatgaaga agtcggtgga aagccttcag cgcgagttcg
     1321 ctaccttgcg gaccggccgg gcatctgctt ctttgcttga tcggattaga gtcgattact
     1381 acggtgcgga gaccccgctc aatcaggttt cgacgatcag cacccccgag gcccgcacga
     1441 tcgtcattca accatgggat aaaagcctgc ttggcgtcat cgaaaaagcg attctgaaaa
     1501 gcgatctgga cctaccgccg aacaatgacg gaaagcttat ccggctcaac ttccccccat
     1561 tgaatgagga gcgacggaag gctttggtga aaaccgccaa gtcgatcgcc gaacaatccc
     1621 gcgtcgccat tcgaaatatt cgccgcgaag cgatcgatga tctgaaaaag cttgagaaat
     1681 cgagtgaaat cagtgaagat gagcaaaggg acggtgagac tcgagttcaa aagttgactg
     1741 attcctacat tgaacagatc aacgccctct ctgatgaaaa agagaaggag attatggaga
     1801 tctaacggat gagtgacgac cggcttggtc tgaaacacct tgggataatc atggacggaa
     1861 acggacggtg ggcgaagaag cggaacctgc cgcggaccgc cggccatctt gagggattga
     1921 aggcgctgaa gcgagttatc attgaagcgg cgaaccaaca gatcgaatgg gttacgtttt
     1981 atgccttttc aacggaaaac tggaagcgca ctgaagaaga agtatcctat ctgatgcggc
     2041 tgttcaccag taaac
//
LOCUS       DAOPUZ010000234         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_59337_length_2042_cov_2.562154, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000234 DAOPUZ010000000
VERSION     DAOPUZ010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59337_length_2042_cov_2.562154"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..393)
                     /gene="yajC"
                     /locus_tag="PKN79_04505"
     CDS             complement(25..393)
                     /gene="yajC"
                     /locus_tag="PKN79_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNZ95057.1"
                     /translation="MTTFITFGLIIAIFYFLIIRPQKKREKETKEMLASIKKGDKVVS
                     IGGIHGTVMIVKETTVVIKVDDNTRIEFSRNAISSIVDRKSDAPTARKKAKKTEEVAV
                     VEAEEVVAVEAEEVEEKSEK"
     gene            complement(556..1656)
                     /locus_tag="PKN79_04510"
     CDS             complement(556..1656)
                     /locus_tag="PKN79_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ95058.1"
                     /translation="MEKKSVSVTLNNVTKIFKDPKGKADVLAVDNADFVVEPGELVTL
                     LGPSGCGKTTTLRMIGGFELPTSGKVYIGSDDVTMLPPNRRDTATMFQSYGLFPHMSV
                     FDNVAYGLRLRKTEPDAIRKQVNEYLALVGLAGYGERAPGRLSGGQQQRVALARSLIV
                     TPSVLLLDEPLSNLDALLREQMRVEIKRIQRSLGITAVYVTHDRVEAMSLSDRVIVMK
                     DGKIRQIGAPDVIYEKPDSRFVAGFVGKAAFFPVTIGERKEGVWHCMLGDKVVHARSA
                     APDIVKNSEGVLMARPESLRLVDKGEGHLDGRVRMNVYLGHSLESYIQTAFGEVLVQV
                     DDPNARTIRKEGEEVSIEFPPDRVRLLGKSEE"
     gene            complement(1665..>2042)
                     /locus_tag="PKN79_04515"
     CDS             complement(1665..>2042)
                     /locus_tag="PKN79_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ95059.1"
                     /translation="PVGLENGITALHQIDPSIEEAAHDLGADVPTVFRTIVLPLVRPA
                     FISSMSYTFVRAMTAVSAIIFLTSAKWYHLTVKILSYSENIRFGLASVLCTVLIIIVL
                     GVFGLMQLLVKDEKLTQKSIAMR"
BASE COUNT          414 a          603 c          540 g          485 t
ORIGIN      
        1 tttcatgatt atctccaggg accgttactt ttccgatttc tcttcgactt cttcagcttc
       61 gacggcaacg acttcttcag cttcgacaac ggcaacctct tccgttttct tcgccttttt
      121 ccgagcagtc ggtgcatcgc tctttcgatc gacgatcgag ctgatcgcgt tgcgggagaa
      181 ctcgattcgg gtgttgtcat ccactttgat gaccacagtg gtctctttga cgatcatcac
      241 cgttccgtgg attccgccga tcgaaacgac tttgtcccct ttcttgatcg aggcgagcat
      301 ctccttcgtc tccttttccc gctttttctg cgggcggatg atcaggaaat agaaaatcgc
      361 aataatgagt ccgaacgtaa tgaacgtggt catcatcgag ccaccaccgg cggcggacgc
      421 ctcgggagct ggcatggcag ttaacaatga aagcatgggt attcctcact ttgcaatgaa
      481 atcgggtgcc gtgggcaccc aatttttcac gtaccgctta acgtaccatg ttcacttgtc
      541 gatagtcaag ttttcctact cttcgctttt accgaggagg cggacgcggt ccggcgggaa
      601 ctcgatggag acctcctccc cttcctttcg gatcgtccgg gcgttggggt catccacttg
      661 gacaaggacc tcgccgaagg cggtctgaat gtatgattca agactgtggc cgagatacac
      721 gttcatccgc acccttccgt cgaggtgtcc ctccccttta tcaaccagtc gcaggctctc
      781 cggccgggcc atcaatactc cttcggagtt cttgacgata tccggagcgg cggaccgtgc
      841 gtgcacgacc ttgtctccca acatgcagtg ccacactccc tctttccgct ccccgatcgt
      901 tacggggaag aacgccgctt tgccgacgaa gccggcgacg aagcgggaat cgggtttttc
      961 atagatcaca tcgggagctc cgatctgccg aatctttccg tccttcatca cgatcacccg
     1021 atcggaaagg ctcatcgctt cgacgcggtc gtgggtgacg tacaccgcgg tgattcccaa
     1081 ggaccgctgg atccgtttga tttccaccct catctgctcg cgcaggaggg cgtccaggtt
     1141 ggaaagcggc tcatcaagca ggaggaccga gggggttacg atgagcgacc gggcaagagc
     1201 aacccgctgt tgctggccgc cgctgagcct tcccggggcc cgctcgccgt aaccggcaag
     1261 accgaccagc gccagatatt cattcacctg cttcctgatc gcatccggct cggtttttct
     1321 cagcctcagg ccgtaggcga cgttgtcgaa gacgctcata tggggaaaga ggccgtagct
     1381 ttgaaacatc gtagcggtat cgcggcggtt gggcggcagc atcgtgacat cgtcgctgcc
     1441 gatatacacc ttgccgctgg tcggaagctc gaagcctccg atcatgcgca gcgtcgtcgt
     1501 ctttccgcat cccgagggtc cgagcagggt taccagttcc ccgggttcga cgacgaagtc
     1561 ggcgttgtca acggccagga catccgcctt gcctttcggg tctttaaaaa tctttgtaac
     1621 attgttcaac gttactgaaa cactcttttt ttccatactc gatcctaccg catcgctata
     1681 cttttttgag tcaacttttc atccttgacg agcagctgca tcagaccaaa cacaccgaga
     1741 acgatgatga tgaggacggt acacaacacg ctggccaaac cgaatcggat attctccgaa
     1801 taggagagaa ttttcaccgt caagtgatac cacttcgccg aggtaaggaa gatgatcgcg
     1861 ctgaccgccg tcatcgctcg cacgaaggtg taggacatgc tcgaaatgaa cgccggtctc
     1921 accaggggaa gaacgatcgt cctgaagacg gtgggaacat ccgcaccgag gtcgtgggcc
     1981 gcctcttcga tcgaagggtc gatctgatgg agcgcggtga tgccgttctc caaacccacc
     2041 gg
//
LOCUS       DAOPUZ010000235         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_59556_length_2037_cov_4.164480, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000235 DAOPUZ010000000
VERSION     DAOPUZ010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59556_length_2037_cov_4.164480"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..488
                     /locus_tag="PKN79_04520"
     CDS             33..488
                     /locus_tag="PKN79_04520"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNZ95060.1"
                     /translation="MKMTALYQSPLGPLYFIEEDERLVEVGFGERAETSTTPLLREAM
                     RQMDAYFNDTLTRFDLPLHPRGTEFQLKVWEALKTIPYGQTRSYRDIARIVGNVKAVR
                     AVGGANNKNPISIIIPCHRVIGKSGKLIGYGGGLEKKAWLLAFEKRTPS"
     gene            complement(525..962)
                     /locus_tag="PKN79_04525"
     CDS             complement(525..962)
                     /locus_tag="PKN79_04525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269054.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNZ95061.1"
                     /translation="MVNMEFIGMAAALTLSALGSGIGAGIASMAAVGGWKKCYVENKP
                     APFIMVAFAGAPLTQTIYGFLLMNFIANAIAGGIASTLALFVGVLGGLAIGLSAYMQG
                     KAAAAACDALAETGKGTANYFIVIGIVETVALFTLVFSLLALQ"
     gene            complement(1002..>2037)
                     /locus_tag="PKN79_04530"
     CDS             complement(1002..>2037)
                     /locus_tag="PKN79_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNZ95062.1"
                     /translation="LLEDVSEDDEDVPTLIEYRKGVGIIKPVFDILGTVPGYREHDIS
                     TWFLLFFTLFFAMIIGDAGYGLIFLLIAGAIHISMKKANTLVMLVYVLSIATILWGSL
                     TGTWFGSIEILQSIPFLQKLVIPQISNYPELFGIEAVTAQNTVMKFTFIIGTVQLSLA
                     CIINVVRKARIKDLSLVADLSWLIALLALYYIVLLLVIGAQVNIKALFATVGVAFVTI
                     LLFGAQGPGVSFIDGIKGGLAGFFTTFLDTISAFSNIMSYIRLFAVGMASVAIAQSFN
                     AMASGMLKGFALPAGILVLVIGHGLNLVMGLLSVVVHGVRLNLLEFSGQLGMEWTGIQ
                     YQPFAETVEE"
BASE COUNT          533 a          560 c          535 g          409 t
ORIGIN      
        1 caagaggatc gccggtcaat tatcctatca tcatgaagat gacggccctc tatcaaagcc
       61 cgctcggccc gttgtatttc attgaagaag atgaacgcct cgttgaagtc ggattcggcg
      121 aacgggcgga aacatccacc accccattgc tcagggaagc gatgaggcag atggatgcat
      181 atttcaatga caccctcacg aggttcgacc tgccgctcca cccgagaggc accgagtttc
      241 agctgaaggt gtgggaagcc cttaaaacga ttccctacgg ccaaacgcgt tcctaccggg
      301 atatcgcccg aatcgtcgga aatgtcaaag cggtccgggc cgtcggcggg gcgaacaaca
      361 aaaacccgat ctccatcatc atcccctgtc accgggtgat cgggaaaagc ggcaaattga
      421 tcggttacgg cggcggcttg gagaaaaaag catggctgct ggcatttgaa aagcggacac
      481 cgtcgtgatg tccgctttcc tacccgttgt tcggctaaaa aagattattg caacgcaagc
      541 aagctgaaga caagggtaaa gagagcaacc gtttcaacga taccgatgac gatgaagtag
      601 tttgcggttc cctttccggt ttcggcaaga gcatcacatg cagctgcagc agcctttccc
      661 tgcatatatg cggaaagacc gatcgccaat ccgccgagaa caccgacaaa caaagccaat
      721 gtcgaagcga tgccgccggc aatcgcattt gcgatgaagt tcatcaggag gaagccgtaa
      781 atcgtctgag tgagcggagc tccggcaaac gcgaccatga tgaagggagc gggcttattc
      841 tcgacataac acttcttcca gccgccgacc gcagccatgg aggcgattcc ggcgccgatt
      901 cccgatccga gagcagaaag ggtcaaagcc gctgccattc cgataaattc catattaacc
      961 atctcgttct ccttgtgttg ttccaaacaa cggtgaggta tttattcttc gaccgtttcc
     1021 gcgaacggtt gatattgaat cccggtccac tccatcccga gttgtccgga aaactccaac
     1081 agattgagcc gaacgccgtg aaccacgacg ctgagcagac ccatgaccag attgagtcca
     1141 tgaccgatga ccaataccag gattcccgcc ggaagcgcaa atcccttgag cattccgctt
     1201 gccatcgcat tgaaggattg ggcgatcgcc accgatgcca ttccgacggc aaagaggcga
     1261 atgtagctca taatattgct gaaggcgctg atcgtgtcca ggaacgtggt gaagaaaccg
     1321 gccaagcccc cctttatccc atcgataaac gatactcccg gtccttgcgc tccgaagagc
     1381 aggatcgtca cgaaagcgac tccgaccgtg gcaaagagcg ccttgatatt cacttgcgct
     1441 ccgatgacca gcagtaggac gatgtaatag agcgccagaa gagcgatgag ccaactgaga
     1501 tccgccacca gactgagatc tttaatcctg gccttgcgaa ccacattgat gatacacgcc
     1561 aaagaaagct ggacggttcc aatgatgaag gtgaatttca tcacggtgtt ctgggcagtg
     1621 accgcctcga tgccgaacag ctcggggtag ttggagatct gggggatcac cagcttctgc
     1681 aggaacggaa tgctttgaag gatttcaatg gatccgaacc acgttccggt gagtgagccc
     1741 cagaggatcg tggcgatcga gagcacatag acgagcatca ccagcgtatt cgctttcttc
     1801 atggagatat ggatcgcacc cgcaatgagc aggaagatca atccgtagcc ggcatctccg
     1861 atgatcatcg cgaagaacag ggtgaagaac agaaggaacc acgttgaaat gtcatgttcc
     1921 cggtatcccg gcaccgttcc gaggatatcg aacaccggct tgatgatgcc gacacccttc
     1981 ctgtactcga tcagggtcgg cacatcttca tcatcctcgc tgacatcctc aaggaga
//
LOCUS       DAOPUZ010000236         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_59613_length_2036_cov_2.851590, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000236 DAOPUZ010000000
VERSION     DAOPUZ010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59613_length_2036_cov_2.851590"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PKN79_04535"
     CDS             <1..419
                     /locus_tag="PKN79_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNZ95063.1"
                     /translation="GGPPNTTYGENIGVLALTRVYSVWVTGGAAVIAVLLSFFPKFGA
                     LIQTIPSPVLGGISMLLFGIIASSGLRTLVDSGVDYQDKRNLTISSVILVIGIGGGAL
                     SFAMGRDLMFTLEGVALATVVGIFLNLVLPRNGKHE"
     gene            535..1407
                     /locus_tag="PKN79_04540"
     CDS             535..1407
                     /locus_tag="PKN79_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNZ95064.1"
                     /translation="MHEYTVIDSDEVLAKLRNTWKENRVTTIAMDFEGEYNLHIYGEH
                     LCLIQIFDQTTFYLIDPFEISIPELKRFLEDETLEKIMFDCASDAALVRKNHEITLKK
                     IYDLRIAAKQLGMDGGLSKVLDHYLPDRMRRTSGSKKKHQQTNWLMRPLSREQIQYAL
                     EDVEHLFSLKALIIADLEKRGLKEKTQALMESAGLPKGPDRPAWTKYPAYRYLSKEER
                     ILLKHYYLAREHVAKRRNVPAVRIMNKKLVLKMAKEKPQSREEFESYTQRNDLLTALS
                     EAHAKAMKEIVSLA"
     gene            complement(1368..>2036)
                     /locus_tag="PKN79_04545"
     CDS             complement(1368..>2036)
                     /locus_tag="PKN79_04545"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNZ95065.1"
                     /translation="EEMRPIVGPATTILTLLNGIESEGVLAEAFGWEKILHGFAIGLN
                     STHVGNRIEFTAEGRIVFGERDNSPTDRVAQLSELFASAGIDAVVPRDIRLMQWNKFM
                     LNTAYNTISGLLSATYEQLDQKAVKELAYGVCAEVQAVALAEGVTIPDSFVEENHNLV
                     ITLGDGKTSMCQDLEAGRVTENEWFAGSVVALGEKHGIPTPICQTLSLLVQAKETISF
                     MALA"
BASE COUNT          460 a          594 c          566 g          416 t
ORIGIN      
        1 ggggcgggcc tccgaacacc acctacggtg agaatatcgg tgttcttgcc ctcacccgcg
       61 tgtactccgt gtgggtgacc ggaggtgctg cggtgatcgc ggtcctgctc tccttcttcc
      121 ccaagttcgg agcgctaatc cagacgatcc ccagtcccgt cctcggcggg atttcaatgc
      181 ttctcttcgg catcattgct tccagcggtc tgagaacgct ggtcgacagc ggtgtcgatt
      241 accaggataa gcgcaacctc acgatcagca gcgtgatcct cgtcatcgga atcgggggag
      301 gagccctctc cttcgcgatg ggcagggatc tcatgttcac cttggagggg gttgccctgg
      361 cgaccgtcgt gggcatcttc ctcaacctcg ttcttcccag gaacgggaag catgagtgac
      421 gagcatgcaa cactcggaac atcataaaga agaacaatcg aaagaatcat gtccatcggg
      481 gagccgaccg gctccccggt attctttcat cggcttttct gctacccttg agtgatgcat
      541 gagtatacgg taatcgacag cgatgaagtc cttgccaagc tgcgaaacac atggaaggag
      601 aaccgcgtca ccacgatcgc gatggatttc gaaggggagt acaaccttca catctacggc
      661 gagcacctct gcctcatcca gatctttgat cagacgacct tctacctgat tgatcccttt
      721 gagatctcga tccccgaact gaagcggttt ctggaggatg aaacgctgga aaagatcatg
      781 ttcgattgcg ccagcgatgc cgccttggtt cgaaagaacc atgagatcac cctgaagaag
      841 atctatgacc tcaggatcgc cgcgaagcag ctgggaatgg acgggggact ctcgaaggtg
      901 ctcgatcact accttcccga ccgaatgcgg cgcacgagcg gatcgaagaa aaagcatcag
      961 cagaccaact ggctgatgcg gccgctttcc cgagaacaga tccaatacgc cctggaggat
     1021 gtggagcatc tcttttccct caaagccctg atcatcgccg atcttgagaa gcggggactc
     1081 aaggagaaaa cccaagcatt gatggaaagc gccgggctgc caaaaggacc cgatagacca
     1141 gcctggacga agtatcccgc ctaccgctac ctctcaaagg aggaacggat tttgctgaag
     1201 cattattatt tggcgcgcga gcacgtcgcc aagcgaagga acgtgccggc ggtccgcatc
     1261 atgaacaaga agctcgtctt gaagatggcg aaagagaagc cgcagagccg ggaagagttt
     1321 gagtcctaca cgcaacgaaa cgatctgctt acggcgctgt cggaagctca cgccaaagcc
     1381 atgaaggaga tcgtctcctt ggcctgaacg agaagcgaaa gggtttggca gatcggggta
     1441 ggaatcccgt gcttctcgcc caaagcgacc accgatccgg cgaaccactc gttttccgtc
     1501 acccgacccg cttccagatc ctggcacatc gaggtctttc cgtcgcccaa ggtgatgacg
     1561 aggttgtggt tctcctcgac gaaggaatcg gggatggtga ccccttcggc caacgccacc
     1621 gcctgcactt cggcgcatac cccgtaggcg agctctttca ccgccttctg gtcaagctgt
     1681 tcgtaggtcg ccgacagcag cccgctgatc gtattgtacg cggtgttaag catgaacttg
     1741 ttccactgca tcaggcggat gtccctcggt acgacggcat cgatgcccgc agaagcaaag
     1801 agctccgaca gctgagcgac ccgatccgtc ggggaattgt cccgttcgcc gaagacgatt
     1861 ctcccttcag cggtgaactc gatccgattg cccacatggg tggagttcaa gccgatggcg
     1921 aagccgtgaa gaatcttctc ccacccgaac gcttcagcca ggactccttc actttcgatt
     1981 ccgttcagca gggtgagaat cgtcgtggcg ggaccgacga tcggtctcat ctcctc
//
LOCUS       DAOPUZ010000237         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_59712_length_2034_cov_2.051036, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000237 DAOPUZ010000000
VERSION     DAOPUZ010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59712_length_2034_cov_2.051036"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..>2034)
                     /locus_tag="PKN79_04550"
     CDS             complement(179..>2034)
                     /locus_tag="PKN79_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ95066.1"
                     /translation="NSTKRHFATLNAVLSGVKELRFEIDAARERLELYQRGTILFVDE
                     VHRWNKSQQDALLPWVENGTVILIGATTENPFFEVNAALVSRSRIFQLKPLTDKDLMK
                     IAHMTLSDKERGYGNYEITFEEGALEHLVDVSDGDARTLLNALELAVETTGEAGKPKY
                     ISLAAAEDSIQQKAVLYDKEGDYHFDVISAFIKSIRGSDPDAALYWLARMVHAGEEPR
                     FIFRRMLISAAEDIGLADPNALVVVEAAAAAFERIGLPEGRFHLAQAALYLATAKKSN
                     STMGFFDALKKVTEEAQREGPNHLRDNSRDAKGFGHGEGYLYPHAYSGHWVAQQYLPA
                     ALQGKIFYNPGDQGYEATIASEVQGRREEQLESIQSDVFVEHLSYSPPSSVEAIWRER
                     SAGERSTILRAIRKELFREHTVRRSDRVFIANAGSGFLLWEALRLTPEGLCVAQVSTK
                     QHAEVINHYAKTLEMLERPLVHIGDPASVLRSLHERLLFEHIYGRNILSRLHAEKEIL
                     ELFFEKLVKGGTLNIAQSIPSRSSRLSEFCDHPLLKEAEQVIYADETNPLINWDEKDL
                     EEAAKSYGFQGKTEVLSFTENRVFTSDQLEGYLKNSYLPASRTRIPPSSFQ"
BASE COUNT          403 a          546 c          590 g          495 t
ORIGIN      
        1 cttccaccag gaagctgaag cagtggcctg gaccagtgcg attcaacgtt gcttactatg
       61 ccaaaaacct acttctttgt caacaaccat aaatgacggg tgattgttca atccttggca
      121 aggcgcagga tcgcgatgct ttgacggtag gcgaggctct tcttctcaag agctttgatc
      181 actgaaacga cgaaggaggg atcctcgttc ttgaggcggg cagataggag ttcttcagat
      241 atccctcaag ctgatccgag gtgaagaccc ggttttcggt gaatgagagc acttccgtct
      301 ttccttggaa tccgtacgat tttgctgctt cttccagatc cttctcgtcc cagttgatca
      361 gggggttggt ttcgtcggca tagatgactt gttccgcttc tttgagcagc ggatgatcgc
      421 agaactcgct taatcgggag gaacgggagg ggatgctttg ggcgatgttc agggtgccgc
      481 ctttcaccag tttctcaaaa aaaagttcca atatttcttt ctccgcatgc aagcgggaga
      541 ggatgtttcg tccgtagatg tgttcaaaga ggagtcgttc atgtagggag cggaggaccg
      601 aagccgggtc tccaatgtga acgagcggcc gttccagcat ttcgagcgtt tttgcatagt
      661 gattgatcac ttccgcgtgc tgtttcgtgg acacctgggc tacgcacaag ccctccgggg
      721 tcaggcgaag tgcttcccag agaagaaagc cgctgcccgc gttggcgatg aaaacccgat
      781 cgcttcggcg aaccgtatgt tcgcgaaaga gctccttcct gatcgccctc agaatcgtcg
      841 accgttcacc cgccgatcgc tctcgccata ttgcctcaac cgacgaaggg ggagaatagg
      901 agaggtgctc gacaaacacg tcgctttgga tcgattctag ctgttcctcc cgccgccctt
      961 ggacctcgga agcgattgtc gcctcgtacc cctgatcgcc cggattgtaa aagatcttcc
     1021 cctgcaaggc tgccggcaga tactgctggg cgacccagtg accgctgtag gcgtgtgggt
     1081 agagatatcc ttcaccgtgc ccgaacccct tggcgtcgcg gctgttgtcg cgaaggtggt
     1141 taggaccttc gcgttgagct tcctcggtga cttttttcaa cgcgtcgaag aatcccatcg
     1201 tcgagttgga ctttttcgcg gttgcaaggt agagggcggc ctgagcgagg tggaagcggc
     1261 cttccggcag accgattcgt tcaaacgccg cggcggcggc ctcgaccacc accagtgcgt
     1321 tggggtcggc aagaccgatg tcttcggcgg cgctgatcaa cattcttcga aagatgaacc
     1381 gcggctcctc gccggcgtgg accattcggg caagccagta gagggcggca tcgggatcgc
     1441 tgcccctgat tgatttgatg aacgcgctga tcacgtcaaa gtgataatcc ccttccttgt
     1501 cgtagagaac cgctttttgc tggatcgaat cttcggcggc ggcgagggag atatactttg
     1561 gcttgcccgc ctcgccggtt gtttcgactg caagctcgag ggcgttcaag agggttcggg
     1621 catcgccgtc gctgacatct acgaggtgct ccaacgcccc ctcttcaaag gttatctcat
     1681 agttgccgta gccgcgttcc ttgtcgctta acgtcatatg ggcgatcttc atcagatcct
     1741 tatcggtcag cggcttgagc tggaagattc ttgagcggga taccaaggcg gcattgactt
     1801 cgaagaaggg gttctccgtc gtagcgccga tcaggatgac cgtcccgttt tccacccaag
     1861 gaagcagggc atcctgttgg cttttattcc aacgatgcac ctcgtcgaca aagaggatcg
     1921 ttccgcgttg atacagttca aggcgttcgc gtgccgcatc gatttcaaaa cggagttctt
     1981 tcactccgct gaggacggcg ttcaaggtgg cgaagtgccg cttggtcgag ttgg
//
LOCUS       DAOPUZ010000238         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_60196_length_2023_cov_3.388211, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000238 DAOPUZ010000000
VERSION     DAOPUZ010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60196_length_2023_cov_3.388211"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKN79_04555"
     CDS             complement(<1..219)
                     /locus_tag="PKN79_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95067.1"
                     /translation="MLHYAVYQKNSAIVYTLKKRVRMVFLLITLFLLWGLVLNIGEGV
                     SIRSMIHIVLLLIVTTIGVLYRDGWTFDV"
     gene            407..>2023
                     /locus_tag="PKN79_04560"
     CDS             407..>2023
                     /locus_tag="PKN79_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ95068.1"
                     /translation="MKKFLVITLSILLVGALFISCGGKKEAPAAAAPAAAPAAPAPAA
                     PAAPAPAAPAAPAPAPAKPVAAAPVDEVVFRLTNGAEPESLDPALVQGVPEHRIYMAI
                     FEGLVAMDPKTGNAIPGVAESWTGNEDGTQWTFKLRKNAVWSDGKAITAHDVVWSWLR
                     ILDPATGGPYAWFPAMFLVGAEEYNAGLAPASAVGIRALDDYTFQMDLLGPLPYAIDA
                     LTHYSFAIMPKHAIEKHGAAWTEPANFVGNGPFILADRVPQTSITVTKSPTYWDKANV
                     RLDKVIFYSSDSDTTNYNMYLNGETDWATNVPTDQIAAAEMRDDFHKSPQLATYYYVF
                     QTEKAPLDNPLVRKALAYSVDREALIEGITKAGQIPAWGIVPDMAGYKALEFPFDSMD
                     EAVEQAQKWLAQAGYPNGAGFPTVSILYNTSEGHKQIGEFIQQEWKKNLGINVVLENQ
                     EWGTYLNSRNVGDFQISRAGWVGDYQDPNTFLDMFITGTAMNGGQYSNEVYDILINEA
                     ARIDNPADRFGVLRTAEDIMINEDQALMNLY"
BASE COUNT          487 a          607 c          526 g          403 t
ORIGIN      
        1 cacatcaaag gtccagccgt cgcgatagag gactccgatc gttgtgacaa tcagaagcag
       61 gacgatgtgg atcatcgacc gaatggaaac accttcacca atgttgagaa ccaatcccca
      121 cagtaaaaac agggtgatca gaaggaacac catccgtaca cgctttttca acgtatagac
      181 gatcgccgaa tttttttgat agactgcata gtgtagcatg aggtactccg tctcaagtat
      241 accgatatta aaccctttgt taaggcttgt ttatgcgctg atttgcatat ccatacacca
      301 aaagcatgcc atcctctatg ctttttcctt gattcatgat taaatgagga ttaagcttca
      361 tggtatcgaa tagtgaatac cctaacgatg ataaggagag atgtttatga agaagtttct
      421 tgtgattact ttatctatcc tgcttgtcgg agcgctcttt atttcctgtg gaggaaagaa
      481 ggaagctcct gcagcagcag caccggcagc cgctcctgca gcaccggccc cggcagcacc
      541 cgcagcaccg gccccggcag cacccgcagc accggccccc gctccggcca agccggtagc
      601 agcagcaccg gttgatgagg ttgtcttcag acttaccaac ggcgccgagc ccgagtccct
      661 cgatccggct cttgttcagg gtgttcccga gcaccggatc tacatggcga tcttcgaagg
      721 cctcgttgcc atggatccca agaccggtaa tgcgatcccc ggcgttgctg aaagctggac
      781 gggcaatgaa gacggcaccc agtggacctt caagctgcgc aagaacgcag tctggtccga
      841 tggcaaggcg atcaccgccc acgatgtcgt ctggtcctgg ctgaggatcc tcgatcccgc
      901 aaccggcgga ccgtacgcct ggttcccggc gatgttcctc gttggagccg aagagtacaa
      961 cgcagggctt gccccggcga gcgccgtcgg aatccgcgct ctggatgact ataccttcca
     1021 gatggacctc ctcggaccgc ttccgtacgc aatcgacgcg ctgacccact acagcttcgc
     1081 gatcatgccc aagcacgcga tagagaagca cggcgcagcg tggaccgagc cggcgaactt
     1141 cgttggcaac ggacccttca tccttgccga ccgcgttccc cagacctcga ttaccgtcac
     1201 gaagagcccg acctattggg acaaagccaa tgtgagactg gacaaggtca tcttctactc
     1261 cagcgattcg gacaccacga actacaatat gtacctcaac ggtgagaccg actgggcgac
     1321 caacgtaccg accgaccaga tcgcagcggc cgagatgcgg gacgacttcc acaagtctcc
     1381 gcagcttgcc acctactact acgtcttcca gaccgagaag gctcctttgg acaaccccct
     1441 tgtccggaag gctctcgcct attcggtcga ccgcgaagcg ctgattgaag gcatcaccaa
     1501 ggccggacag atccccgctt ggggtatcgt cccggatatg gccggctaca aagcgcttga
     1561 gttcccgttc gattcgatgg acgaggcggt tgaacaggcc cagaagtggc ttgctcaagc
     1621 cggatatccc aacggtgccg gtttcccgac cgtttcaatc ctctacaaca cgagcgaagg
     1681 acacaagcag atcggcgagt tcatccagca ggagtggaag aagaacctcg gaatcaacgt
     1741 cgttcttgag aaccaggagt ggggaaccta cctgaacagc cgaaacgtcg gagacttcca
     1801 gatctcccgc gccggatggg ttggcgacta tcaggatccg aacaccttcc tcgacatgtt
     1861 catcacggga acagcgatga acggcggcca gtactccaac gaggtgtatg acatcctcat
     1921 caacgaggct gcccgaatcg acaatcccgc cgaccgcttc ggagttctca gaaccgctga
     1981 agacatcatg atcaacgaag atcaggctct gatgaacctc tac
//
LOCUS       DAOPUZ010000239         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_60224_length_2023_cov_2.094004, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000239 DAOPUZ010000000
VERSION     DAOPUZ010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60224_length_2023_cov_2.094004"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PKN79_04565"
     CDS             <1..481
                     /locus_tag="PKN79_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNZ95069.1"
                     /translation="AKLVHAVDVGYNLIDWKLRSDERVIVHERQNIMTLAEPSPPPTA
                     AVCDLSFRSIRKAASHILDLVGNTYLIALIKPQFEMENPSPSFDGVIEDPGLIVQVVT
                     QLFLRLAEESVGVFDLVASPIRGAKGNVEFLALLKKGQGLSLKLLTHQVEAQLELLQ"
     gene            complement(441..971)
                     /locus_tag="PKN79_04570"
     CDS             complement(441..971)
                     /locus_tag="PKN79_04570"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNZ95070.1"
                     /translation="MANHPKALLRALIIFLLLILYTTIIGIVSFPTVKRAFHESGVLQ
                     LIRNPKEQTQASLSKRMVSVVFAPLGTSFAVYTVEQPRLGSSASHDTFEALLSGVPLT
                     ALKDGAITYIASGTKLLGLTRSSNIIYLNLSKEFLASSEMELAYRQLKETASLLGAKD
                     IVVLIEGAQVELQPDE"
     gene            1046..>2023
                     /locus_tag="PKN79_04575"
     CDS             1046..>2023
                     /locus_tag="PKN79_04575"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HNZ95071.1"
                     /translation="MRIFTGLAIGEGIARGKAKIYRHTPIAVEREQIAKSQVEGEHSL
                     LDQAFRRARTALERRKGGHLEGEALEILETHLLLLQDPTYIKSIKEMIEEKLINAAWA
                     TEEVTRGIIHMLQASDDEVFRQRAIDIRDIGDSIVVHITGKGAHPSLVLDEPVILLAD
                     TLLPSEFFSLDRSNILGLSLDRGGLASHVAILARGLDIPTVIETHPASHAARDGDDVI
                     IDGIRGEVIIRADRQAAQNVIERYNAYLKHEAELLEGASLEARTKDGKHIPIQSNIQT
                     LGELSFAEAEGSDGIGLFRTEFFFMERSTLPSEEEQFRVYKEVLERVAPR"
BASE COUNT          478 a          543 c          580 g          422 t
ORIGIN      
        1 ggccaagctg gtccatgccg tggacgtggg atacaatctg atcgattgga agctgcgcag
       61 cgacgagcgg gtgatcgtcc atgaaaggca aaacatcatg actctcgccg agccgagccc
      121 ccctccgacg gcggcggtgt gcgacctttc cttccgttcg atccgcaaag cggcgtcgca
      181 catcctcgac cttgtaggaa acacctacct gatcgccctc atcaaaccgc agtttgaaat
      241 ggaaaaccct tcaccctcgt ttgacggtgt catcgaagac cccggcctca ttgttcaggt
      301 ggtcacgcag ctctttctcc gtctggccga ggagagtgtg ggagtcttcg atcttgtggc
      361 aagccccatc agaggagcaa aggggaatgt cgagttcctt gccctcctga aaaaggggca
      421 ggggctctca ctcaaactcc tcactcatca ggttgaagct caacttgagc tccttcaata
      481 aggactacaa tatccttcgc cccgagcagt gaagcggttt ctttgagttg acgatatgcc
      541 agctccatct ccgatgaagc gagaaactcc tttgaaaggt tcaggtagat gatgtttgag
      601 gaacgggtaa ggcccaagag cttcgtgccc gatgcgatgt atgtaatcgc tccatctttc
      661 aacgcggtca atggaactcc cgagagcagc gcctcaaagg tatcatggct tgccgacgat
      721 cccaggcgcg gctgctcaac cgtatagacg gcgaacgagg tgccaagcgg agcaaagacg
      781 acgctcacca tccgtttgga caaggatgct tgggtttgtt ccttcggatt ccggataagc
      841 tgaagaaccc cgctttcatg aaaggcgcgc tttaccgtcg ggaagctgac aattccgatg
      901 atcgtcgtat acaggatgag aagcaggaag atgataaggg cccgaaggag ggcttttggg
      961 tggttggcca tcgtcttatc gtagcaaaga gtgggctctt cttccagctt tacatcacgg
     1021 agcaaactcc gtatcatacc ggtatatgag gatttttacg ggtttggcaa tcggtgaagg
     1081 aatcgctcgg ggtaaggcaa aaatctatcg ccataccccg atcgccgttg aacgggagca
     1141 gattgccaag tcccaggtgg aaggtgaaca cagtctcctc gatcaggcgt ttcgacgcgc
     1201 ccgcacggcc cttgagcgac gcaagggcgg tcatttggaa ggagaagcgc tggaaatcct
     1261 ggaaacccac ctcctcctgt tgcaagatcc gacgtatatc aaatcgatta aggaaatgat
     1321 cgaagagaaa ctcatcaatg ccgcatgggc taccgaagag gttacccggg gaatcatcca
     1381 catgctgcag gcaagcgatg atgaagtgtt tcgacagagg gcgatcgaca tcagagatat
     1441 cggggactcg atcgtcgtac acatcaccgg caaaggcgct catccttcgt tggttctcga
     1501 tgagccggta atccttctgg cggacacgct gctgcccagc gagttcttca gcttggatcg
     1561 ctcaaacatt ctcggcctga gtttggatag aggcgggctg gcaagccacg tggcgattct
     1621 cgcccggggg ctggacatcc ccaccgtcat cgaaacgcat ccggcctccc atgcggctcg
     1681 agatggcgat gatgtgatca ttgacggaat ccggggtgaa gtgatcatcc gggcggatcg
     1741 ccaggcggct cagaacgtca tcgaacgtta caacgcctac ttgaagcacg aagcggaact
     1801 cttggaagga gcgagcttgg aggcccggac caaggacggg aaacacatcc ccattcaatc
     1861 caatatccaa accctcggcg agctgtcctt tgccgaggcg gagggcagcg acgggatcgg
     1921 tctcttcaga acggagttct tcttcatgga gcgttcgacg cttccaagcg aagaggagca
     1981 gttccgggta tacaaggaag tgctcgagcg ggtcgctccg aga
//
LOCUS       DAOPUZ010000240         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_60269_length_2022_cov_2.326894, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000240 DAOPUZ010000000
VERSION     DAOPUZ010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60269_length_2022_cov_2.326894"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PKN79_04580"
     CDS             complement(<1..843)
                     /locus_tag="PKN79_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNZ95072.1"
                     /translation="MSDVLQRLMNQEVLLATVSGVGSTKAELIRYFDESVEAIDLITT
                     KSFQNEPNPGNREPIICEVEKGTFGNSVGLRNPGLEVALHELRELRKTHSFRALLNVS
                     LSASTPEDFVTLVKAFDEVADLVELNFSCPHAEPGYGSTIGENLTLAAHYVRFIKANT
                     VECEAPLFVKLTPNVEDIASIAKAVVKAGADGIVAINTVGPLLYIDPVAGKPILQNRL
                     GGKGGQSGKAIFPIALKAIKEIREALGTGIPIIGMGGVDSGADAAKLIEAGANVVGIG
                     SALGT"
     gene            complement(840..1706)
                     /gene="pyrF"
                     /locus_tag="PKN79_04585"
     CDS             complement(840..1706)
                     /gene="pyrF"
                     /locus_tag="PKN79_04585"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNZ95073.1"
                     /translation="MTYRELLETSAKQVGNVVCMGLDVKPELLPHAEQGIRHRLNTYF
                     QQLFRRMNLSGLLPAAFKPNIGYYAVLDNPREEEFSGSLALADLFDLLENFFPNIPII
                     LDSKRGDIATSSRNYADEAFKRWRANAITVAPYMGSDSVGPFIQDSTFGTYILNRTSN
                     PGGKDLQNLILHSGIPLYEAVAKQIIAYNAGKGHVGAVVGATNPEELKAIAKTYAEQS
                     IPLLIPGVGSQGGSATDVMRTLGEVNYPLPLVRINSSSALTHPWKKGPAPDDWLDQCE
                     GSLRTLIEECSL"
     gene            complement(1703..>2022)
                     /locus_tag="PKN79_04590"
     CDS             complement(1703..>2022)
                     /locus_tag="PKN79_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNZ95074.1"
                     /translation="THVLLIEDLISTGLSSTSAVEAIVAAKGQCPYTLAIFTYGFAAA
                     EEAFAALNPKAAFHTILTYEVMLSSALKEGYISRSEAESLEAWSSDPFGWGIERGFMR
                     EEG"
BASE COUNT          399 a          600 c          561 g          462 t
ORIGIN      
        1 cgtgccgaga gcggatccga tgccgacgac gttcgccccc gcttcgatga gcttcgccgc
       61 atccgcccct gaatcgacac cgcccattcc gatgatcggg atccccgtcc cgagcgcttc
      121 ccgaatctct tttatcgcct tgagggcgat cggaaagatc gcttttccgc tttgcccgcc
      181 ctttccgccg agcctgttct ggaggatcgg cttcccggca acgggatcaa tgtagaggag
      241 cggtccgacc gtgttgatcg caacgatccc gtccgccccc gctttgacga ccgctttggc
      301 gatcgaagcg atatcttcaa cgttcggcgt caatttcaca aacaggggcg cctcgcattc
      361 gacggtgttc gccttgatga agcgcacgta atgggcggca agcgtaaggt tctccccgat
      421 cgtcgagccg taccccggct cggcgtgcgg gcaggagaag ttcagttcca caagatcggc
      481 gacttcatca aacgctttga cgagtgttac gaagtcttcg ggcgtggagg cggagagcga
      541 cacgttcaac agagctcgga aggagtgggt tttcctgagc tctcgaagtt catggagggc
      601 cacctccagc ccgggatttc tcagccctac cgagtttccg aacgttcctt tctccacttc
      661 gcagatgatc ggttcgcggt tccccgggtt cggttcgttc tggaagctct tggtggtgat
      721 gagatcgatc gcctccaccg actcatcgaa gtagcggatc agttccgctt ttgtggaacc
      781 caccccgctg acggtggcaa gaagaacctc ttgattcatc aagcgttgaa gcacatcact
      841 catagcgagc actcctcgat gagagttcgc agagaaccct cgcattgatc gagccagtca
      901 tccggcgccg ggcccttttt ccacggatgg gtcaaggcgc tggagctgtt gatccgaacc
      961 aatggcaagg gatagttcac ctcgccgagg gtcctcatca catcggttgc ggagcctccc
     1021 tggcttccca ctcccggaat caacagcgga atcgactgct cggcatatgt tttggcgatt
     1081 gctttcagct cctccggatt ggttgccccg accaccgctc cgacatgccc cttcccggca
     1141 ttgtaggcga tgatctgctt ggccaccgct tcatagagcg gaatcccgct gtgcaggatg
     1201 aggttctgca gatccttccc tccgggattg ctcgttcgat tgagaatgta tgttccaaac
     1261 gtggaatcct gaatgaacgg tccgacggag tcgctgccca tatagggggc gacggtgatc
     1321 gcgttcgctc tccaccgttt gaacgcttca tcggcgtagt tccggctgct tgtcgcaatg
     1381 tcaccacgct ttgaatcgag gatgattgga atattgggaa agaagttttc caagagatcg
     1441 aagagatcgg ccagggcgag cgaacctgag aactcctcct cccggggatt gtccagcaca
     1501 gcatagtagc cgatattcgg cttgaacgca gccggcagga ggcccgaaag attcatccgg
     1561 cgaaagagct gttggaagta ggtgttcagc cggtggcgga ttccctgttc agcatgggga
     1621 agaagctccg gcttcacatc caagcccata cacaccacgt ttccgacctg ctttgcactt
     1681 gtttcaagca attccctgta cgtcatcctt cctccctcat gaagccccgc tctattcccc
     1741 atccgaaggg atcgctgctc catgcctcga ggctttccgc ctcggatcgt gagatatacc
     1801 cttctttcag ggcgcttgag agcatcactt cataggtgag aatcgtatga aatgccgctt
     1861 tcgggttcaa tgcggcgaac gcttcttcag ctgccgcaaa cccataggtg aagatcgcca
     1921 gcgtataggg gcactgcccc ttggccgcga cgatcgcttc gacggcgctc gtcgatgaaa
     1981 gaccggtgga gatcagatct tcgatcaaaa ggacatgggt gt
//
LOCUS       DAOPUZ010000241         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_61125_length_2003_cov_3.927618, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000241 DAOPUZ010000000
VERSION     DAOPUZ010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61125_length_2003_cov_3.927618"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..601
                     /locus_tag="PKN79_04595"
     CDS             62..601
                     /locus_tag="PKN79_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95075.1"
                     /translation="MKHVTAVLLLMFIGTHVVFGSTNVELMTDCTSLISLFGETISAR
                     IEARLDLSDEFALMLPVELMAEKGRGQPIYLESGLFLAYHPFSKAAAIYASLIQLGIL
                     FERRYGETKTFFLNEVGFGYTFRLPFGLLIEPRMTIKDPNSVFTSDYQELKETFSSYP
                     MIRFALYGGWSFTLPSATT"
     gene            614..1459
                     /locus_tag="PKN79_04600"
     CDS             614..1459
                     /locus_tag="PKN79_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95076.1"
                     /translation="MKRWMNTIVAILLIIPISGCTMGLEMKKKHAVTEEQIISGVSAI
                     LDAQKETVWPYLAEEMDVLSRGGIPSAEEIIRNTLAEELGMEYLEFCYVVGTGETQED
                     LNLVTEYAKTLLDEEGKAELERSLVQVREILLAEAGDLARRLAPSQRVPFWKDMQKLV
                     TRTMVLFTAGIVYACMPTTVFWGKVSAAAAIAVAAGIVSETVMAIWRYYQFGGDPDEA
                     FGEWLKGVTTEPELSYALTASVMAVGKTMKRGPVVTGILICVFAIYNVIDIVKPMLKR
                     YNFNV"
     gene            1534..>2003
                     /locus_tag="PKN79_04605"
     CDS             1534..>2003
                     /locus_tag="PKN79_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HNZ95077.1"
                     /translation="MITRLKEDILKRFLRYVQVDTMSDDRVVDTKRPSTDGQWDLLKM
                     LASELVELGVDDVHIDEKGIVIAKIPSNMKKAVPVVGFMAHVDTADDVPGNGVKPRVI
                     EKYDGGDIPLNEEHSIIAVENPELSDYVGETLVVTDGTTLLGSDDKAGIAEIMSA"
BASE COUNT          445 a          517 c          614 g          427 t
ORIGIN      
        1 agagcatagg gcactttgct tacctacaga atagacgtaa tggtgtgaag ctcacgaaga
       61 gatgaaacac gtcacagcgg tattgctcct catgtttatc ggaacacatg ttgtcttcgg
      121 ctccacgaat gttgagttga tgaccgattg cacctcgttg atctcgctct tcggcgagac
      181 gatttcggct cgcattgaag cccggctcga tctgagtgac gagtttgctc tgatgctgcc
      241 cgttgaactg atggcggaaa agggcagggg gcaaccgatc tatctggaga gcggcctgtt
      301 tctcgcctac catccgtttt ccaaggcggc ggcaatctat gcatctctca tccagttggg
      361 aatcctcttt gaacggcggt acggggaaac gaagacattc tttctgaacg aggtgggctt
      421 cggctacacc ttccgtcttc ccttcggact gctcattgag ccgcggatga ccataaaaga
      481 tcccaacagt gttttcacaa gcgattatca ggaattgaag gagaccttca gttcctatcc
      541 gatgattcgg ttcgctctct acggcgggtg gtccttcacc ctgccgtcgg caacaaccta
      601 agaggaggaa gtgatgaagc gatggatgaa tacgatcgtg gcaatcctgt tgatcatccc
      661 catttcgggg tgcacgatgg ggttggagat gaagaagaaa cacgctgtga cggaggagca
      721 gatcatcagc ggagtctcgg caatccttga tgcccagaag gagaccgtct ggccgtacct
      781 ggccgaggag atggatgtcc tgagtcgagg gggcattccg tcggcggagg agatcattcg
      841 aaacaccctt gccgaagagc tggggatgga gtatctggag ttctgctacg tcgtcggcac
      901 cggggagact caggaggacc tgaatctggt gacggagtac gcgaaaacgc tcctggacga
      961 ggagggaaag gcggagctgg aacgttcgct tgtccaagtc agggagatcc tgcttgccga
     1021 ggcgggggat ctggccaggc gccttgcacc cagtcaacgg gtgccgttct ggaaggacat
     1081 gcagaagctc gtgacccgga cgatggtcct cttcaccgcc ggaatcgtgt atgcgtgcat
     1141 gccgacgacg gtcttctggg ggaaggtgtc cgccgccgcg gcgatcgccg tggccgcggg
     1201 gatcgtcagc gaaacggtaa tggcgatctg gcggtactac cagttcggcg gcgatcccga
     1261 tgaagcgttc ggcgagtggc tcaaaggggt tacgaccgag cccgagctct cctatgcgtt
     1321 gaccgccagc gtgatggcgg tgggcaaaac catgaagcgg ggaccggtcg tgacgggaat
     1381 cctcatctgt gtttttgcga tctacaacgt catcgacatc gtaaaaccga tgctgaaacg
     1441 gtacaatttc aacgtctagg ccgtctcgtt tcctcctcaa tgttgatgag gtccgcactt
     1501 tgtgtgatac tgaatcaatc tgaggaggaa gatatgatca cccggttgaa agaagatatt
     1561 ctcaagcggt ttctccgcta cgtccaagtc gacacgatga gcgacgatcg cgtcgtcgac
     1621 accaagcgcc cctctaccga cgggcagtgg gatctcttga agatgctcgc ctccgagctt
     1681 gtcgaacttg gagtcgatga tgtgcatatc gatgagaagg ggatcgtcat tgcgaagatt
     1741 ccctccaaca tgaagaaagc ggtgccggtc gtcggcttca tggcgcacgt cgataccgcc
     1801 gatgatgtgc cgggcaacgg ggtaaagccc cgggtgatcg agaagtatga cggaggggac
     1861 atccccctca acgaggaaca ctcgatcatc gcggttgaaa accccgagct ttccgactat
     1921 gtcggagaga cgctcgtcgt caccgacggg acgaccctgc tcggtagcga cgacaaggcc
     1981 ggaatcgccg agatcatgag cgc
//
LOCUS       DAOPUZ010000242         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_61134_length_2003_cov_2.768994, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000242 DAOPUZ010000000
VERSION     DAOPUZ010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61134_length_2003_cov_2.768994"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PKN79_04610"
     CDS             <1..1152
                     /locus_tag="PKN79_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNZ95078.1"
                     /translation="RGKKIALIGPLGNHPYAMYGGYTAPVHLQGSAKWEETVPKRSKT
                     IKEALETYAEVEFQPGCVIFESSIERSIFFPGEVEQQDGPLAKVPSTDTSRIIDAVKT
                     AQNADVVVLVVGDLVGLFQQGTTGEGSDANTLRLPGVQEELMEAVLATGKPTVVLLVS
                     GRPYTVNSAVKRAGALVAAWLPGQGGGEAIAQTLFGEKNFSGKTTLSFPFSAGAMPYT
                     YNHSKKSGGLPRQSAFGARYPFGFGLSYTSFTYPVAKLKTDTVDLDGEVEVLVTVKNT
                     GAVDGEEVVQLYIHDRHASLVRPVKELKGFARVPLKAGEEKTVTFTFKTDLLSFYKNG
                     KRILEGGEFDLFIGSSSRDIHHSFLLTVKDGVRILDFNSWNDRSQVMIT"
     gene            complement(1211..>2003)
                     /locus_tag="PKN79_04615"
     CDS             complement(1211..>2003)
                     /locus_tag="PKN79_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNZ95079.1"
                     /translation="NSRDHRKVIVIDGNIGYTGGMNIADEYINRTIKYGHWKDTAVKI
                     TGDAVWSLTQMFLQLWSFSTGVTEQFALYQPTITAKTDGFVQPFGDNPLDSHNVAENA
                     YIQVINMAKRYVYITTPYLVLDNEMLTALKIAAQSGVDVRIVTPYIPDKKGVFLVTRA
                     NYYKLLEAGVKIYEYTPGFIHAKMFVSDDHVAIIGTINMDYRSFYLHFENAIVLYGSS
                     AIKHVYEDIERTIMLGKPITTKLLKARPWHQRLGGMILKIAAPML"
BASE COUNT          465 a          542 c          540 g          456 t
ORIGIN      
        1 agaggtaaga agatcgccct catcggccct ttgggcaatc atccatatgc aatgtacggc
       61 ggctataccg caccggtcca ccttcaaggt tccgccaagt gggaagagac cgttcccaag
      121 agaagcaaga cgatcaagga agcgctcgaa acctatgccg aagtcgagtt ccaaccggga
      181 tgcgtcatct tcgagtcctc aatcgaacgg tcgatcttct tccccgggga agtcgaacag
      241 caggacggtc ccttggcgaa agttccttcg accgatacca gccggatcat cgatgcggtc
      301 aagaccgctc agaatgccga tgtcgtcgtc cttgtcgtcg gagatctggt cggcctcttc
      361 cagcagggaa cgaccggtga gggcagtgat gccaacaccc tcaggctccc gggagtccag
      421 gaggagctga tggaagcggt gcttgccaca ggcaagccca ctgtcgttct tctcgtcagc
      481 ggtcggccgt acaccgtcaa cagcgcggtt aaacgggccg gagcgctggt ggcagcatgg
      541 ctgcccggcc aaggcggtgg agaagcgatt gcccagactc tcttcgggga gaaaaacttc
      601 agcggcaaga ccaccctctc ctttcccttc agtgccggtg cgatgccgta tacctacaat
      661 cacagcaaga aatccggcgg tctgccccgc caaagtgcgt tcggtgcccg atatccgttc
      721 ggattcggtc tttcctacac ctcctttacc tacccggtag cgaagctcaa gactgatacg
      781 gtagaccttg acggcgaagt cgaggtgctc gttacggtaa agaatacagg tgccgtcgat
      841 ggtgaagagg tcgtgcagct ctacatccac gaccgtcacg cttccttggt gcggccggta
      901 aaagagctca aagggttcgc tcgagtcccg ctcaaagcag gagaagagaa gaccgtgacc
      961 tttaccttca aaaccgatct cctctccttc tacaagaacg gaaagcggat tcttgaagga
     1021 ggtgaattcg atctcttcat cggatcgtca agtcgggaca ttcatcattc cttcctcttg
     1081 acggtcaagg acggagtgcg aatccttgat ttcaattcat ggaatgatcg ctcacaggtg
     1141 atgattacat aaacgcaaaa agcaacccat gcagaaacac ccggcatacc gcatgtcggg
     1201 tgtttccctg tcaaagcatc ggagctgcga tcttgagaat catcccaccc agtctttgat
     1261 gccagggccg agctttgaga agcttggtcg taatcggttt acccaacatg atcgtacgtt
     1321 cgatatcttc atacacgtgc ttgattgccg aggagccata aaggacgatc gcattttcga
     1381 agtgaagata gaacgaacgg taatccatgt tgatcgtccc gatgatcgcg acatgatcgt
     1441 cgctgacgaa cattttggca tggatgaatc cgggggtgta ttcatagatt ttcaccccgg
     1501 cttcaaggag tttgtaatag ttcgcccgcg taacgaggaa cacgcccttc ttgtcgggga
     1561 tgtagggagt cacgattcga acatccactc cgctttgggc ggcgatcttc aaagcggtca
     1621 gcatctcatt gtccaacaca aggtaggggg tcgtgatata gacgtagcgt ttggccatgt
     1681 tgatcacctg gatatacgcg ttctccgcta cgttgtgact atcaagggga ttatccccga
     1741 agggctggac gaagccgtcg gtcttcgccg taatcgttgg ctgatacagg gcgaactgtt
     1801 cggtgactcc cgttgaaaag gaccagagct gaaggaacat ctgggtcaac gaccagaccg
     1861 catcgccggt gattttgacc gctgtatctt tccaatgccc atatttgatc gttcgattga
     1921 tatactcatc ggcgatattc attccgccgg tatatccgat gttcccgtca atgacgatca
     1981 ccttccggtg atcgcggctg ttg
//
LOCUS       DAOPUZ010000243         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_61942_length_1985_cov_3.969948, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000243 DAOPUZ010000000
VERSION     DAOPUZ010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61942_length_1985_cov_3.969948"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1518
                     /gene="prs"
                     /locus_tag="PKN79_04620"
     CDS             223..1518
                     /gene="prs"
                     /locus_tag="PKN79_04620"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNZ95080.1"
                     /translation="MSIIKPQKLGIISGPGSEYFTGKVVKHLRRLYLERYYKLSSALA
                     KRHGISEEEILKTVTLTDDLSNKRIPRSKAPTTFQCPDFTIKTKYVRFANGEVKAEIL
                     DPVRGLRVFIIHDLSNNEPVRVSGSDEPLRLTINDHLVFLLTTINALTLAGADSVTLV
                     IPIYPYSRQHKKSTREALTAAMLGRIFEMLKVERIITLDIHSREIENSFGHLHLENLH
                     ASYQTLIALRKLVDFSDPNLVVVAPDTGAISRNKFYAQALHRPLAMLYKERDYSVVSK
                     DARNSNIKSINLLGDVKGKTVLIADDMLATGGTMIVAMRELTNLGAEKIICMVSLPYF
                     NGTAVEDFDQAYREGIFYRIIGTNAVTHGRDLLDKEWYIQADITELFARVISRLHHGR
                     SISPLLDNRRFIQILIDNTQANPKAPLPDAAATDPDQED"
     gene            1587..>1985
                     /locus_tag="PKN79_04625"
     CDS             1587..>1985
                     /locus_tag="PKN79_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ95081.1"
                     /translation="MKMSNRISSFQASPIRRLSPYARSAVKRGITVYHLNIGQPDIKT
                     PQVVLDAIRSYNEPIIAYGSSEGDERLRNALPAYYATYGVDIKPEDILITTGGSEALL
                     FTLMTLCDPGDQVIIPEPFYTNVSSFAHSAM"
BASE COUNT          490 a          568 c          493 g          434 t
ORIGIN      
        1 taaacaaaaa actacgctca cctagttttg gttagttact gcggaagcat ggagcttcat
       61 tccatacagg aaaagaccca gacttccaat tatctggaaa aagatcatga ctgctttcat
      121 accggctcaa ccctaacaag tttaacccaa gaggtaaagt aggagaccgt caacggttca
      181 gtgtggattg aactttaaaa gcccttgttg tatggttggg ctatgagcat catcaaaccg
      241 caaaagcttg gaatcatatc agggccgggc tcggagtatt ttaccggaaa agttgtcaag
      301 catcttcgga ggctctattt ggaacggtac tacaagcttt ccagtgctct tgccaagcgg
      361 cacggcatca gcgaggagga gatccttaaa accgtcaccc tcaccgatga cctttccaat
      421 aaacggattc cccgctcaaa ggcccccacc accttccaat gccccgattt cacgatcaag
      481 acgaagtatg tgcggtttgc aaacggtgaa gtgaaagccg agattctcga tccggtgcgg
      541 ggtctcaggg tcttcatcat ccatgacctt tcgaacaatg aaccggtgcg agtcagcgga
      601 tccgacgagc ccctgaggct tacgataaac gaccacttgg tgtttttatt gaccacgatc
      661 aatgcgctca ccctggccgg ggcggattcc gtaacgctgg tcattccaat ctatccctat
      721 tcccgccagc ataaaaagag cacgcgggaa gcgctgaccg ccgcgatgct gggtcggatc
      781 ttcgagatgc tcaaggtcga gcggatcatc acgctggaca tccactcccg ggagatcgag
      841 aactccttcg gacaccttca tctggagaac ctccacgctt cctatcagac gctcatcgct
      901 ctgcgcaagc tcgttgattt cagcgatccg aacctggtgg tggtcgcccc ggatacgggg
      961 gcgatcagcc ggaacaagtt ctacgctcag gcgctccacc gtccgcttgc gatgctctat
     1021 aaggagcgcg attactcggt ggtcagcaag gatgcccgaa actccaacat caaatcgatc
     1081 aacctgctcg gagacgtcaa aggcaagacc gtcctgatcg ccgacgacat gctggctaca
     1141 ggcggaacga tgatcgtagc gatgcgcgag ttgaccaacc tcggagcgga gaagatcatc
     1201 tgcatggtca gtcttccgta tttcaacgga acagcggtgg aggacttcga tcaagcgtat
     1261 agagaaggca tcttctatcg gatcatcggg acaaacgccg tgacgcacgg ccgcgatctg
     1321 ctggataaag agtggtacat tcaggcggac atcaccgagc tttttgcccg ggtgatcagc
     1381 cgccttcacc acggccgctc gatcagcccg ctgttggata accgccggtt catccagatt
     1441 ctcatcgaca acactcaagc caatccaaaa gctcctcttc cggatgctgc cgctaccgac
     1501 ccggatcaag aagattaatc tttgatccgg gtgagtcagc agcctccctt acgggtggta
     1561 accatcatac cataggagtg accatcatga agatgtccaa ccgaatttca tcatttcaag
     1621 cttctccgat ccgccggctt tcgccgtatg cccgttcagc cgtaaaacgg gggatcaccg
     1681 tatatcacct gaatatcggc caacccgata tcaagactcc ccaagtcgtt cttgatgcga
     1741 tccggagcta caacgagccg atcatcgcct acggctccag cgagggagac gagcgccttc
     1801 gaaacgcgct tcccgcctat tacgcaacat atggagtgga tatcaagccg gaggatattt
     1861 tgattactac cggcgggagc gaagcactgc tctttacgct gatgacactc tgtgatccgg
     1921 gcgatcaggt gatcatcccc gaaccctttt atacgaacgt ctcctccttt gcccactcgg
     1981 cgatg
//
LOCUS       DAOPUZ010000244         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_62349_length_1977_cov_3.506764, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000244 DAOPUZ010000000
VERSION     DAOPUZ010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62349_length_1977_cov_3.506764"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1977)
                     /locus_tag="PKN79_04630"
     CDS             complement(<1..>1977)
                     /locus_tag="PKN79_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNZ95082.1"
                     /translation="ALMEKASQEQQRIMEQYRQGHITQEERYNRVIEVWTQTNEQLTD
                     ELMEELRKDQQGFNPLFLMADSGARGSKTQIRQLGGMRGLMARPSGDVIEFPIKSNFK
                     EGLSIIEFFISTNGARKGLSDTALKTAEAGYLTRRLVDISQDVVVNEDDCNTINGIWR
                     GAIKDGDEIVESISERIVGRVPVEDILHPFSREVLAKANVEIDEATALAIEESGLESV
                     LLRTVLTCEAKHGVCAKCYGRNLATNRPVVIGEAVGIIAAQSIGQPGTQLTMRTFHVG
                     GTASTSAEENKLTFNYPVIIGTISGARVVRESDQINVFTRKGTIEYFRANAIIEEGSY
                     DTLLVEDGSTVAKGTPIYVKKGKNVLSKENGSVRIVDGRVFLLGHSTLHTVKAGSELL
                     VDTGAFVEAKRTIVTFDPFSEPILAEESGFASFVDIKLGTTLIEEVNEETGNIEKKIT
                     EHSLESLQPRIEITSEKKGKGDVLAVYLLPGGSYIQVADNAKVTKGMILAKLLKEGIK
                     TKDITGGLPRVGELFEARRPRNAAILAKVSGLVSFGNIVKGKRTIAVTDSYGHEFKHM
                     VPMGRNLLVRDGDNVEAAEALCDGQIDPHDILEILGENALQSFLVDEVQEVYRMQGVN
                     INDKHLGVIVRQMLRKVEIAHVGDTNLIHGQQVD"
BASE COUNT          356 a          601 c          539 g          481 t
ORIGIN      
        1 tgtcgacttg ctggccatgg atcagattcg tatcgccgac gtgagcaatc tccaccttgc
       61 gcagcatctg gcggacgatc acaccgaggt gcttgtcgtt gatgttcact ccctgcattc
      121 gatagacttc ctgcacttca tccaccagga atgattgcag ggcgttctca ccgaggattt
      181 ccagaatatc gtgggggtcg atctgaccgt cgcacagcgc ttcggcagcc tcgacgttgt
      241 caccgtcgcg aaccagaagg ttgcggccca tcggaaccat atgcttgaac tcatgaccgt
      301 aggaatcggt gaccgcgatc gtgcgcttgc ccttgacgat gtttccaaag ctcaccaatc
      361 cgctgacttt cgccagaatc gcggcgttgc gcgggcggcg ggcctcaaac agttcgccga
      421 cccggggaag accgccggtg atatccttgg ttttgatccc ctctttgagg agcttcgcca
      481 ggatcatgcc cttggtaact ttcgcgttgt cggcgacttg aatgtaggaa ccgccgggaa
      541 ggaggtagac ggcgagtaca tcgcccttgc ccttcttctc gctggtaatc tcaatccgcg
      601 gctgcaatga ttcaaggctg tgttcagtga tctttttctc gatgtttccc gtctcctcat
      661 tgacctcttc gatcagcgtg gttccgagtt ttatatcgac aaacgaggcg aatccgctct
      721 cttcggcgag aatcggttcg ctgaacgggt cgaaggtgac gatcgtcctc ttggcctcga
      781 cgaacgcgcc ggtatcgacc agcagctcac ttcccgcttt gacggtatga agcgtggagt
      841 gtccgagtag gaacactctg ccatcaacga ttcggaccga tccgttctcc ttggagagga
      901 cattcttgcc cttcttcacg tagatggggg ttcccttggc gacagtcgag ccgtcttcaa
      961 ccaaaagcgt atcgtagctt ccctcttcaa tgatcgcgtt ggctcggaaa tactcgatcg
     1021 tccccttgcg ggtgaagacg ttgatctgat cgctttcgcg cacaactctc gccccgctga
     1081 tcgtaccgat gatcaccggg taattgaagg tgagcttgtt ttcttccgcg ctggtcgagg
     1141 ccgttccacc aacgtggaac gttctcatcg tcagctgcgt tcccggctgg ccgatcgact
     1201 gcgcagcgat aattcctacg gcttcaccga tcaccaccgg gcgattcgtc gccagattgc
     1261 gcccgtaaca cttggcacac accccgtgct tcgcctcgca ggtgagaacg gttcgaagga
     1321 gcacgctctc caagcccgac tcctcgatgg caagagcagt cgcttcgtcg atctcgacgt
     1381 tcgccttggc gagcacttcg cggctgaaag gatgaaggat gtcctcgacg ggaacccgtc
     1441 cgacgatccg ttcgctgatc gattcgacga tctcatcccc atccttgatc gcaccgcgcc
     1501 agatgccgtt gatggtattg cagtcatcct cgttgactac cacgtcctga ctgatatcaa
     1561 ccagacgccg ggtcagatag cccgcttcgg cggtcttcaa tgcggtgtcg gagagaccct
     1621 tgcgggctcc gttggtcgag atgaagaatt cgatgatcga aagaccttcc ttgaagttcg
     1681 atttgatcgg gaattcgatg acgtctccgc tggggcgggc catcaggccg cgcattccgc
     1741 cgagctggcg gatctgcgtc ttcgatcccc gagcgccgct gtcggccatc aggaagaggg
     1801 ggttgaaccc ctgctggtct ttcctcagct cctccatcag ctcgtcggtg agctgctcgt
     1861 tcgtctgggt ccacacctcg atgacgcggt tgtaccgctc ctcctgggtg atatgaccct
     1921 gccgatactg ctccatgatc cgctgctgct cctggctcgc cttttccatc agcgctt
//
LOCUS       DAOPUZ010000245         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_62831_length_1967_cov_3.406904, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000245 DAOPUZ010000000
VERSION     DAOPUZ010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62831_length_1967_cov_3.406904"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PKN79_04635"
     CDS             <1..499
                     /locus_tag="PKN79_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ95083.1"
                     /translation="GATKEAMVWGFRIGFLTYGAKGLTDRQHDALNKKTLAAIRTSVS
                     NCDRPGQSLLIKAMQEGQRYEIDKQAVFSEMKQRYLVLKETLKRFEGDENLIPYPFNS
                     GYFMAFKTRKSAEELRRHLLDRYEVGTINIADTTLRVAYCSVDLEKVGELIELVYRAA
                     GEIWN"
     gene            490..867
                     /locus_tag="PKN79_04640"
     CDS             490..867
                     /locus_tag="PKN79_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNZ95084.1"
                     /translation="MELKSKLYLVNEQGEKFMGIGVLWLLEEIARCSSLRKAALVLGI
                     SYSKAYGMIENLEKGLDVKMLERKRGGSSREGATLTKAAERFIGIYRTFHLEAKERLE
                     APYERLVEAMKSVMEEDDEHKEL"
     gene            848..>1967
                     /locus_tag="PKN79_04645"
     CDS             848..>1967
                     /locus_tag="PKN79_04645"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNZ95085.1"
                     /translation="MSTKSYDFTISSLGKAKVPNPIIMGDKHGDVQVDYVRDSDHILF
                     GIEAVMNEVGRQVPRFEETVELAGPREKIFFNPKHVHAAIATCGGICPGLNNVIRSVV
                     RCFWYRYGVRRISGIQYGYQGLLASSPWPIIPLDPDVVDDIQEKGGTILGSARGGGKQ
                     VEEIVDSLERLNVNILVTVGGDGTLRGARDIAEEVGRRGLKIAVIGIPKTIDNDLSLI
                     QSSFGVDTAVQMAVPVVRGAHVEAKNSIHGIGLVKVMGRESGFIAANTSLAQSDVNFC
                     LIPENPFDLEGPNGFLAHLKKRVLDRDHAVVLVSEGAGQDLLPETGEVDASGNPKFHD
                     IGIYLKDEIIKYFKKEGIEVNLKYIDPSYIIRSAPADAD"
BASE COUNT          484 a          492 c          584 g          407 t
ORIGIN      
        1 cggggcgacg aaggaagcga tggtctgggg gttcaggatc ggctttctca cctatggagc
       61 gaaaggactt accgaccgac agcacgatgc attgaacaaa aagacgctcg ccgcgattcg
      121 gacctcggtt tccaactgcg atcgtcccgg gcagagcctg ttgatcaagg cgatgcagga
      181 gggacagcgg tatgagatcg acaagcaggc ggtcttctcc gagatgaagc agcgctatct
      241 tgtgctcaag gaaacgttga agcgctttga aggagatgag aacttgatcc catatccctt
      301 caacagcgga tatttcatgg cattcaagac aagaaaaagc gccgaggagc tgaggcgcca
      361 tctcttggac cggtatgaag tgggcacgat caacatcgcc gatactactc tacgcgtcgc
      421 ctattgctcg gtggacttgg aaaaagttgg agagctgata gaattggtct atcgggcggc
      481 aggtgagata tggaattgaa gagcaagttg tatctggtca atgaacaggg cgaaaaattc
      541 atgggcatcg gagtattgtg gcttctggag gagatcgccc gttgttcctc attgcggaag
      601 gccgccctcg ttcttggcat ttcctattct aaagcgtatg gtatgattga gaaccttgaa
      661 aagggactcg atgtgaagat gcttgaacgc aagcgcggcg gatcctcgcg ggagggcgcc
      721 actcttacga aagccgccga acggtttatt ggaatctatc gaacctttca tcttgaagcc
      781 aaggagcggc tcgaggcgcc gtacgaaagg cttgtcgaag cgatgaagag cgtcatggag
      841 gaagatgatg agcacaaaga gctatgattt cacgatttcc agtttaggca aagcgaaagt
      901 ccccaacccg atcatcatgg gggacaagca cggggatgtt caggtggact atgttcgaga
      961 cagcgaccac atcctcttcg gaatcgaagc ggtgatgaat gaggttggcc gtcaagtccc
     1021 ccgttttgaa gaaaccgtgg aacttgcagg gccgagggag aagattttct tcaatcccaa
     1081 gcatgtccat gcggcgatcg ctacctgcgg cggaatctgc ccgggattga acaacgtcat
     1141 ccgatcggtc gtccgctgct tctggtatcg gtatggagta cgtcgaatca gcggaatcca
     1201 atacgggtat caaggcctgc tcgcctccag tccctggccg atcatcccgc tcgatcccga
     1261 tgtggtggat gacatccaag agaagggtgg aacgattctg ggttccgccc gcggcggcgg
     1321 aaagcaagtc gaagagatcg ttgattcgct tgagcggctc aacgtgaaca tcctcgttac
     1381 cgtcggtggc gacggaaccc tccgcggagc gcgggatatc gccgaagaag taggccggcg
     1441 cggtctgaag atcgctgtca tcggcatccc caagacgatc gacaacgacc tttcgttgat
     1501 ccaatcctct ttcggagtgg acaccgccgt tcaaatggcg gttcccgtcg ttcggggagc
     1561 gcacgtcgaa gcaaaaaact cgattcacgg aataggattg gtgaaagtga tggggcggga
     1621 aagcggcttc atcgctgcaa acacctcgct cgcccaaagc gacgtgaact tctgcctgat
     1681 tccggagaac cccttcgatt tggagggacc gaacggtttc ctggcccact tgaagaagcg
     1741 ggttcttgac cgcgaccacg cggtcgtcct cgtcagtgaa ggcgcaggtc aggacctgct
     1801 gcccgaaacc ggcgaagtgg atgcttcggg caatccgaaa ttccacgata tcggaatcta
     1861 cctcaaagat gagatcatca aatacttcaa gaaggaagga atcgaagtaa acctgaagta
     1921 tatcgatccc tcctacatca tccgcagcgc gccggcggat gcggatg
//
LOCUS       DAOPUZ010000246         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_63034_length_1963_cov_2.148323, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000246 DAOPUZ010000000
VERSION     DAOPUZ010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63034_length_1963_cov_2.148323"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /locus_tag="PKN79_04650"
     CDS             <1..1570
                     /locus_tag="PKN79_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNZ95086.1"
                     /translation="STKSMNNGLDFLIEAVQSVFDTKKKQQPLHIKGMKTDASKEEQE
                     QIDKTRFSVVNEIVDRVERRRMDSSEHTFQDTIDRVVANKWLGIPIFALVIWVVFSIS
                     QSWLGPWLADLFVGWIDAFYGLVESVLGENVHPVLSSLLLDGIIGGVGAVVGFLPLIM
                     VLFFMLALLEDSGYMARVALVMDPYFKKIGLSGKSIIPMIVSTGCAIPGVMATRTIKN
                     ERQRRTTALLAPFMPCGAKLPIIALFAGVFFGDNVWVGTSMYFLAIFVIILGGLIIRK
                     ITGDTSKSYFIMELPEYRIPSIKRATISMLNRAKAFIVKAGTIILISNAVVHMLQTFN
                     WRFQVVGTSAPETSILASIATPMAWLFIPLGFGIWQFAAAAVTGFIAKENVVGTLAVT
                     FAITNFIDVEEFALISGGSDVMTVFGIGAVAALSYLVFNLFTPPCFAAIGSMNSEMES
                     PKWLWGAIAFQLGMGYVLSFFIYQIGTLVTTGSVGSGFLGGLIAVLAMIGYVVYLMRK
                     GSSKQMIPMAQEVA"
     gene            1572..1724
                     /locus_tag="PKN79_04655"
     CDS             1572..1724
                     /locus_tag="PKN79_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HNZ95087.1"
                     /translation="MTLMDILVGAIIAGMVGAAAWYLFRNKKKGGTCTGCSCCPLAEG
                     CGSRKE"
     gene            complement(1725..>1963)
                     /locus_tag="PKN79_04660"
     CDS             complement(1725..>1963)
                     /locus_tag="PKN79_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ95088.1"
                     /translation="FRFPMLFLCGLFIPISSLPLVLRPLSFCLPLTYGTDLLRGAITG
                     VRAFTPVISFGMLLVFSVALFLISRCKIDRKWIL"
BASE COUNT          407 a          536 c          591 g          429 t
ORIGIN      
        1 ttccaccaag tccatgaaca atggcctcga tttcctgatc gaagcggtcc aaagcgtctt
       61 cgatacgaag aagaaacagc agccgcttca catcaaggga atgaagaccg atgcctcgaa
      121 ggaggagcag gagcagatcg acaagactcg cttctcggtg gtgaatgaga tcgtcgaccg
      181 ggtcgagcgc cgcaggatgg attcttcgga gcacaccttc caggatacga tcgaccgggt
      241 cgtcgccaac aaatggctgg ggattccgat ctttgcgctc gtcatctggg tggtgttctc
      301 gatctcccaa tcgtggcttg ggccctggct tgccgatctc ttcgtcggtt ggatcgacgc
      361 tttctacggc ctggtcgaat cggttctggg ggagaacgtg catcccgtgc tctcctcgct
      421 gctgctggac ggaatcatcg gcggtgtagg ggccgtggtc ggcttcctgc ccttgatcat
      481 ggtgctgttc ttcatgctcg cccttcttga ggacagcgga tacatggcgc gcgtcgcgct
      541 ggtgatggat ccgtatttca agaagatcgg gctttcggga aaatcgatca ttcccatgat
      601 cgtgagcacc ggatgtgcga ttcccggggt catggcgacg aggacgatca agaacgagcg
      661 gcagcgaaga accacagcgc tgctggctcc cttcatgccg tgcggagcga agctgccgat
      721 catcgccctc ttcgccggcg tgttcttcgg ggacaacgtc tgggtcggga cgtcgatgta
      781 tttcctggcg atcttcgtga ttatcctcgg cggcctgatc atccggaaaa tcaccggaga
      841 cacctcgaag tcctacttca tcatggagct tcccgagtat cggatcccca gcatcaagcg
      901 ggccacgatc tcgatgctga accgggcgaa agcgttcatc gtcaaagcgg gtacgatcat
      961 cctgatcagc aatgccgtcg tgcacatgct tcagaccttc aactggcggt tccaggtcgt
     1021 cggcacctcc gcacctgaaa cgagcatcct tgcaagcatc gctaccccga tggcctggct
     1081 gttcattcct ctcggcttcg gaatctggca gttcgcagcc gccgcggtca ccggcttcat
     1141 cgccaaggag aacgtcgtcg gaacgcttgc cgtcaccttc gcgataacga atttcatcga
     1201 tgtcgaagag ttcgccctga tctcaggagg ctccgatgtg atgaccgtct tcggaatcgg
     1261 agcggtggcc gcgttgtcct acctggtgtt caacctgttc acacctccgt gctttgcagc
     1321 gatcggatcg atgaactccg agatggagtc gcccaaatgg ctctggggcg cgatcgcgtt
     1381 tcagctgggg atggggtacg tgctttcatt cttcatctac cagatcggca ccttggtcac
     1441 gacgggttcc gtcggttcgg ggttcctggg cggactgatc gccgtccttg cgatgatcgg
     1501 ctatgtggtc tatctgatga ggaaaggctc ttcgaaacag atgattccca tggcccaaga
     1561 ggtggcttga tatgacgctg atggatattc tggttggagc gatcatcgcg ggcatggtag
     1621 gagccgctgc atggtatctg tttcgaaaca agaagaaagg gggcacctgc accgggtgct
     1681 cctgctgtcc gcttgccgag ggctgcggga gcaggaaaga gtagctacag gatccatttc
     1741 cgatcgatct tgcatcggga gatcaaaaac agcgcaaccg agaagaccag caacattcca
     1801 aacgatatca cgggagtgaa tgccctcact cccgtgattg cgcctctcag gagatcggtt
     1861 ccgtaggtga gcggcaggca gaacgaaaga gggcgcagaa caaggggaag gctggaaatc
     1921 ggtatgaaca ggccgcagag aaagagcatc ggaaagcgga aga
//
LOCUS       DAOPUZ010000247         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_63063_length_1962_cov_3.417410, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000247 DAOPUZ010000000
VERSION     DAOPUZ010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63063_length_1962_cov_3.417410"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PKN79_04665"
     CDS             complement(<1..340)
                     /locus_tag="PKN79_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ95089.1"
                     /translation="MNNELGAPLLEMQHISKDYFGNEVLSDINFSLRRGEILGLVGEN
                     GAGKSTLMKILFGMDEIHQTGGYGGSIFIEGDEVRFTSPIDALASGIGMVHQEFSLLP
                     DSTATENILLN"
     gene            complement(459..1670)
                     /locus_tag="PKN79_04670"
     CDS             complement(459..1670)
                     /locus_tag="PKN79_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HNZ95090.1"
                     /translation="MKKTLAIAMVVLFAASMVFAAGSKETAPAKSTAFHVGIVTGTVS
                     QSEDDLRGAEKLIEEYGDVKSGGIINHVTYPDNFMEEQETTISVIAGLADDPLMKAVI
                     VNQAIPGTTEAFRIIKEKRPDIITIAGEPHEDPPVIQTAADLAVNNDFVSRGYLIIKT
                     AHDLGIKTFVHVSFPRHMSYETLSRRAAIMAETAKDLGMRFVYETAPDPVSDVGIAGA
                     QQYILEKVPAWLEQYGKDAAFFVTNDAHTEPLLKQLLAYGGYFIEADLPSPLMGYPGA
                     LGLDLSKEAGDFAAILAKVEKAVVDKDGAGRFGTWAYSYGYVTTAGLGTHAINVIKGE
                     SELTKLSDIMKAYGKYTGSAKWNGAFYNDVTTGVVARNHVLIYQDTYMMGKGYMGAAD
                     VVVPEKYFSIK"
     gene            complement(1784..>1962)
                     /locus_tag="PKN79_04675"
     CDS             complement(1784..>1962)
                     /locus_tag="PKN79_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNZ95091.1"
                     /translation="VFPKRTALVLGSEGRGISQLTEKLCDHIVAIPTTGNIDSLNVSV
                     AAGIFLYEIRRQQA"
BASE COUNT          421 a          534 c          533 g          474 t
ORIGIN      
        1 gattgagcaa aatattctcc gttgccgttg aatccggcag cagtgagaac tcctggtgaa
       61 ccattccgat accactggca agagcatcga tcggcgaggt aaatcgcact tcgtccccct
      121 cgatgaagat cgatcctcca tatccacccg tctggtggat ctcatccatg ccgaagagaa
      181 tcttcatcaa agttgatttg ccggctccgt tttcccctac aagtcccaaa atctccccgc
      241 gtctgagaga gaaattgata tcggaaagaa cttcattgcc gaagtaatct ttgctgatgt
      301 gttgcatctc caaaagaggc gccccaagtt cgttattcat gatgtttcct taactatcca
      361 tggatgtgac caatacctcg aaaagggatt gatcccagcc acaagacgcc caaaaggggg
      421 agagtgacct ctcccccatg caccgggcgc tttggggctt atttgatgga gaagtatttc
      481 tccgggacga ctacgtccgc agctcccata taccctttac ccatcatata ggtgtcctgg
      541 tagatcaaca cgtggttgcg ggcgacgaca ccggtggtca catcgttgta gaatgcaccg
      601 ttccacttgg cgctgccggt gtacttgccg tatgccttca tgatgtcgct gagcttggtc
      661 aattcgcttt cgcccttgat gacgttgatc gcgtgggttc caagtccggc ggtggtaacg
      721 tatccatagg agtacgccca cgttccgaag cgaccggcgc catccttgtc gacgacggcc
      781 ttctcgacct ttgcgagaat cgcggcgaag tcgccggcct ctttggagag gtcaaggccc
      841 agtgctccgg gatagcccat cagcgggctg ggcaggtcgg cttcgatgaa gtatccgccg
      901 tacgccagaa gctgcttcag aagcggctcg gtgtgagcgt cgttggttac gaagaatgca
      961 gcatctttgc cgtactgttc gagccaagcg ggaaccttct caagaatgta ctgctgggca
     1021 ccggctattc cgacatcgct taccgggtcg ggagcggtct catagacgaa gcgcataccg
     1081 agatccttgg cggtctcggc catgatcgca gcccggcggc tgagggtctc atagctcatg
     1141 tggcggggga acgagacgtg gacgaacgtc ttgatgccca gatcatgagc ggtcttgatg
     1201 atcaggtatc cgcgggaaac gaagtcgttg tttaccgcaa gatcggcggc ggtctgaatc
     1261 accggcggat cttcgtgcgg ctctccggcg atcgtgatga tgtcggggcg cttctccttg
     1321 atgatccgga acgcctcggt ggtgccggga atcgcctgat tgacgataac cgccttcatc
     1381 aacgggtcgt ccgccagacc ggcgatgacg ctgatcgtcg tctcctgttc ctccatgaag
     1441 ttgtccggat aggtaacgtg gttgatgatt ccaccactct ttacatcacc atattcttca
     1501 atgagttttt ccgcaccgcg caggtcgtct tccgattgag aaaccgtacc ggtcacgatt
     1561 ccgacgtgga atgccgtact cttggcggga gcggtttctt tggaacctgc agcaaagacc
     1621 atggaagctg caaacagcac gaccatggca atagctaatg tctttttcat tgctactctc
     1681 ctttagcggg atattttccc tatccgtaca taatacgtaa aagcattgat cgcggcaaga
     1741 agagagcacg ggtttatgca agatatgtaa atatggcgta tatttatgcc tgctggcgtc
     1801 gaatttcgta gagaaagatc cccgcggcga cggaaacatt cagtgaatcg atgttgccgg
     1861 tcgtcggaat cgccacgatg tggtcgcaca gcttctccgt caactggctg attccccttc
     1921 cctcgctgcc cagcacaagg gcggttcgtt tcggaaagac cg
//
LOCUS       DAOPUZ010000248         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_63273_length_1958_cov_3.041513, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000248 DAOPUZ010000000
VERSION     DAOPUZ010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63273_length_1958_cov_3.041513"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKN79_04680"
     CDS             complement(<1..704)
                     /locus_tag="PKN79_04680"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNZ95092.1"
                     /translation="MEKKPIDWANLSFGYLPTDWRYVSVYKHGAWDDGGLTDDPNVVM
                     SECAGALQYAQTCFEGLKVYTTVDDRIVAFRPDLNAQRMYETAKRLVMPPFPVDRFLS
                     ALDEVVTANKAFVPPYGSGASLYIRPYMYGSGAIIGVAPAPEYTFRIFTTPVGPYFKG
                     GVKPLRLLVSEYDRAAPNGTGHIKAGLNYAMSLYAGLEAKKQGFDENMYLDSQTRTYI
                     EETGGANFIFVTKDGKV"
     gene            complement(813..1358)
                     /locus_tag="PKN79_04685"
     CDS             complement(813..1358)
                     /locus_tag="PKN79_04685"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNZ95093.1"
                     /translation="MNIYDFSVNGMDGKKVDLSTFSGKALLIVNTATRCGFTPQYTEL
                     EKLYESYRDRGFEILDFPSNQFREQAPEDIDEIDRICRVDYGTEFPRFDKIEVNGAGE
                     NPLYTFLKAEKSFAGFDPSHALGKKIASIVQEADPDYEKNSDIKWNFTKFLVDRKGNV
                     VRRFEPTHSFDDISKAIEAVL"
     gene            complement(1462..>1958)
                     /locus_tag="PKN79_04690"
     CDS             complement(1462..>1958)
                     /locus_tag="PKN79_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNZ95094.1"
                     /translation="HYHALFGDDAPLCYVLEPEKAQCLYQSALADDGKPRSVGGDLDT
                     LMAGLACGDPSPLAWEVIDAYADGFIVIPDELAAKGMRIYASPIPGDPQVKSGESGAG
                     GLGLLATIMGNSAYAGLREQLGLDETSQVLFISSEGDTDPVHYKEIVWEGSSPVSKQY
                     YNEY"
BASE COUNT          434 a          551 c          506 g          467 t
ORIGIN      
        1 acctttccgt ctttcgttac gaagatgaag ttcgcccctc cggtctcttc gatatacgtc
       61 cgggtctgag aatcaaggta catgttttca tcaaacccct gctttttcgc ttccaggccg
      121 gcatagaggc tcatcgcgta gttcaacccc gccttgatgt ggcccgttcc gttcggggcg
      181 gcacggtcat actcggaaac aaggagccga agcggtttca cgccgccttt gaaataggga
      241 ccgacggggg tggtaaaaat tctaaacgta tactcgggag ccggagcgac gccgatgatc
      301 gctcccgatc cgtacatata cggccgaata tacagactgg cgcctgaacc gtagggcgga
      361 acgaacgcct tgttcgccgt caccacctca tcgagagccg agaggaagcg gtcgacgggg
      421 aacggaggca tgacgagccg cttggcggtt tcatacatcc gctgggcatt gagatcgggg
      481 cggaacgcga cgatccggtc atcgacggtc gtgtagacct tcagtccttc gaagcaggtc
      541 tgggcgtact gcaacgctcc ggcgcactcg ctcatgacga cgttgggatc atccgtcaat
      601 cctccgtcat cccatgcacc atgtttgtac accgaaacat accgccaatc ggtaggaaga
      661 tacccgaagc tcaaattcgc ccaatctatc ggctttttct ccacgctcat gcccccaaaa
      721 agatattgag accattgtat cgagcttcat gcacttcgtc tataattatg caatattatt
      781 gtatatagcg tgaaaaagaa atcatcgctt ccctagagaa ccgcttcgat cgccttggag
      841 atatcgtcaa agctgtgggt cggctcgaag cgccgaacga cgtttccctt ccggtcgacc
      901 agaaacttgg tgaaattcca cttgatgtca ctgttcttct catagtcggg atccgcctcc
      961 tgcacgatgg aagcgatctt cttgccgagg gcatgggaag gatcgaagcc ggcaaagctc
     1021 ttctctgctt tcaggaacgt gtacagcgga ttctcccccg ccccgttgac ttcgatcttg
     1081 tcaaaccgag ggaactccgt cccatagtcc actcgacaga tccgatcgat ctcatcgata
     1141 tcctcgggcg cctgctctct gaactggttg ctcgggaaat cgaggatttc aaaccctctg
     1201 tctctatagc tctcatagag cttctccagc tcggtgtatt gcggggtgaa gccgcagcgg
     1261 gtagcggtgt tcacgatcag aagcgccttg ccggaaaagg tcgacaagtc gacttttttt
     1321 ccatccatcc cgttcacgga aaaatcatat atgttcatgt gttgtctcct attatattgc
     1381 cttatattat attttgcaca atatatatgg gaataataaa gctcctcacc tgtcccgtca
     1441 agagatgtga ggagccaaat cttagtattc gttgtaatac tgtttactga ccggtgaaga
     1501 gccttcccaa acgatttctt tgtaatggac cgggtcggtg tccccttcac tggagatgaa
     1561 gagcacctga cttgtttcat caagtcctaa ttgctcgcgc agtccggcat aggcgctgtt
     1621 gcccatgatc gtggccagaa gacccaatcc tccggctccg ctttctccgc tcttcacttg
     1681 aggatcaccg ggaatcggcg aagcatagat ccgcatcccc ttcgctgcaa gctcatcggg
     1741 tatgacgatg aagccgtcgg cgtaggcatc gatgacctcc cacgccagag gagagggatc
     1801 gccgcaggcg aggccggcca tcagggtatc aagatcgccg ccgacgctgc ggggcttgcc
     1861 gtcgtcggcc aatgcgcttt gatagaggca ctgggccttc tccggttcca gaacgtagca
     1921 aagcggagcg tcatccccga agagcgcatg gtagtggc
//
LOCUS       DAOPUZ010000249         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_63536_length_1952_cov_3.962045, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000249 DAOPUZ010000000
VERSION     DAOPUZ010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63536_length_1952_cov_3.962045"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..146)
                     /locus_tag="PKN79_04695"
     tRNA            complement(73..146)
                     /locus_tag="PKN79_04695"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:Asp,seq:gtc)
     gene            252..827
                     /locus_tag="PKN79_04700"
     CDS             252..827
                     /locus_tag="PKN79_04700"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ95095.1"
                     /translation="MKTEIAPLHSKLLHTLRLLLRPPTMADSEAVFAWSSDPEVTRYL
                     RYQTHNSITHTRKIVKSWITQAKDPYFFFWIIEEISTEQAVGSIGIQVVSFNDNHGEV
                     GYCFNRSVWNKGYCTEALKEILRFGFEEARFNRIQASHSTNNPASGRVMEKAGMMREG
                     GPMKDFYKSLQSGYQDTYLYGITKAAWRLLH"
     gene            865..1764
                     /locus_tag="PKN79_04705"
     CDS             865..1764
                     /locus_tag="PKN79_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNZ95096.1"
                     /translation="MQASILRFLHRIATPFLDLLANGASFFGEETFVIAIVLLVFWNI
                     NKQKGFALYMNVLTSVLIMGILKAVVRAPRPFVVLEEIAGKRMETATGYSFPSGHTTT
                     AASFYTSIALLLKKRICSIIAAIMIVLVGVSRLYLGVHWPNDVFAGLLIGVSISFLLY
                     RWSLQLFEDRVRLVRFSIRYGLIATFVSLILSVLLNMELVDPVAFNDLMKTLALAGAG
                     LLGFGFEEQIVRYRVEASLTKKILRYLLGMGVVIAIIASKALFPPSIYAISSFIRYSL
                     VGIWATVLFPITGKSLHLFDDER"
     gene            complement(1724..>1952)
                     /locus_tag="PKN79_04710"
     CDS             complement(1724..>1952)
                     /locus_tag="PKN79_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HNZ95097.1"
                     /translation="WPNLLSYRLSYLVDYFGMEYNAHHALDDALMCGRIFHKQCDGHL
                     DSMFSLRRFLITYKVEPKVIVHHRTGGDFFL"
BASE COUNT          451 a          560 c          473 g          468 t
ORIGIN      
        1 ccgagcaata ctctcatctc ttctccttaa gacagcgaaa ccgccctgga ctccaaggcg
       61 gtttcgaaca gtcgaggatg acgggactcg aacccgcgat ctacggcgtg acaggccgtc
      121 gcgataacca acttcgctac accctcaatg caagatgaac gctacacgat tgctcttctt
      181 ttgtcaacag cttgaaaaga gcccctcacg gttgtcaaga attgagcaag agtatacgct
      241 aagccatcat gatgaaaacc gagatcgcac cgcttcactc caagcttctc cataccctca
      301 ggcttctgct gaggccgccc accatggctg acagcgaagc ggtcttcgcc tggtcgagcg
      361 atcccgaagt gaccaggtat ctgcgctatc agacccacaa ctcgatcacc catacccgca
      421 agatcgtcaa atcgtggatt acccaagcca aagaccccta cttcttcttt tggatcattg
      481 aagagatttc gacagaacaa gctgttgggt cgatagggat tcaggtcgtc tcgttcaacg
      541 acaaccacgg ggaagtcggc tattgcttca atcgttccgt ctggaacaaa gggtactgca
      601 ccgaagcatt gaaagaaatt cttcgcttcg gatttgaaga ggcgcgcttc aaccgaatcc
      661 aggcttccca ctccaccaac aacccggcaa gcggccgggt gatggaaaaa gcggggatga
      721 tgcgggaagg cggtccgatg aaggattttt acaaaagctt gcagagcggc tatcaggaca
      781 cctacctcta cggcatcacc aaagcggcgt ggcgcctctt gcactgagca cacggcgctt
      841 ctatacttcc acacaggagg atatatgcaa gcttcaattc tgcgcttttt acatcggatc
      901 gcaaccccgt ttctggatct gcttgccaat ggtgcaagct tctttggtga agaaaccttc
      961 gtcatagcga tcgttctcct ggtcttttgg aatatcaata agcagaaagg cttcgccctc
     1021 tatatgaacg tcctcacctc ggtcctgatc atggggatcc tcaaggcggt cgtccgggct
     1081 cctcggccgt tcgtcgttct ggaagagatc gccggcaagc ggatggaaac cgccacaggc
     1141 tactccttcc cgagcgggca cacaactacc gcagcctcct tctacacctc catcgccctt
     1201 ctcctgaaga aaagaatatg ttcaatcatc gccgcgatca tgatcgttct cgtcggagtg
     1261 agccgcctct atctcggtgt tcattggccg aatgacgtgt tcgcaggtct tctcatcggg
     1321 gtgagcatca gtttcctcct ctatcgttgg tctcttcagc tttttgagga tcgggtgcgc
     1381 ctggttcgtt tcagcatccg ctacggtctt atcgccactt ttgtctcctt gattctttcc
     1441 gttctgctga atatggaatt agtcgacccc gtggccttca atgacctgat gaaaaccctt
     1501 gcactcgccg gagccgggtt gctcggcttt ggatttgaag agcaaattgt tcgctacaga
     1561 gtcgaagcat cgctgacgaa gaagattctt cgctatctcc ttggaatggg ggtggtcatc
     1621 gccatcatcg cctcgaaggc cctcttcccg ccctcgatct acgccatcag ctcctttatc
     1681 cgctacagcc tcgtaggaat ctgggcgacg gtgcttttcc cgatcacagg aaaaagtctc
     1741 cacctgttcg atgatgaacg atgaccttcg gctcaacctt gtaggtgatc agaaaccggc
     1801 gaaggctgaa catcgaatcg agatgaccgt cacactgttt atggaagatc ctgccgcaca
     1861 tcaatgcatc atccaaggca tggtgggcgt tgtactccat cccgaagtaa tcgaccagat
     1921 atgacaaccg gtaactgaga aggtttggcc ag
//
LOCUS       DAOPUZ010000250         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_63612_length_1951_cov_2.768460, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000250 DAOPUZ010000000
VERSION     DAOPUZ010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63612_length_1951_cov_2.768460"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PKN79_04715"
     CDS             complement(<1..589)
                     /locus_tag="PKN79_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNZ95098.1"
                     /translation="MLCLTLTASTLAENKALVEKNRSWIGAAELRLDHLDEDELKVAS
                     AFPSTVDLPLILTYRRASDGGLCTKNEKDRLQTLYEAAKGGWAYVDIEEDVKKSDLKC
                     KDPLFEGRVDLEESLRARNIRIIRSYHDFEGVPEDLFGRVNRLAGKGDIPKVAVTPQS
                     LLDVIMLFQVEEQLAHIKEKIIIGMGQLGIPTRILY"
     gene            749..>1951
                     /locus_tag="PKN79_04720"
     CDS             749..>1951
                     /locus_tag="PKN79_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNZ95099.1"
                     /translation="MNDISFTLSERARTALVGANGSGKSTLLQIIAGLVSADIYECAH
                     PKEMRISYLPQSDIVLEDGTAFEQVELAYNRFLPLVDEQHSIEERLATGELSDYLLQR
                     LHEIQETLLDEGYHDRKTEIAIILKGLGFHDDDLHRPCSEFSGGWQMRIALAKVLAEK
                     PHLLLLDEPTNYLDIEARYWLRNYLRVYPESVMIVSHDRSFLDETVNEVFELFHGQLK
                     RYKGTYSQYEEQRELEIQSLIHAYEQQQEEIKKQKEFIERFRAKASKARQVQGRIKQL
                     EKLEIIEIPEHLRNLSFSFPPAPHSGKQVLKIENLTKRYGSLTVFEGFDSIITKGERI
                     AVVGKNGTGKSTLLRILAGIDTDYQGSLTVGSGVRIGYFAQDAKQALDDSNTVLDELA
                     SVASTADIP"
BASE COUNT          456 a          533 c          518 g          444 t
ORIGIN      
        1 tgtagaggat tcgcgtggga atccccaatt gccccattcc gatgatgatc ttctctttga
       61 tgtgggcaag ctgttcctcc acctgaaaga gcatgatgac atccaacagc gattgagggg
      121 ttaccgccac tttcgggata tcccccttgc ctgccaggcg attgacccgt ccgaacaggt
      181 cttcggggac cccttcaaag tcatgatagg atcggatgat ccggatattt cgggcgcgta
      241 agctttcttc cagatccacc cgcccttcaa agagggggtc cttacacttc agatcgcttt
      301 ttttcacgtc ctcttcgata tcaacatacg cccacccccc ctttgcagcc tcatagaggg
      361 tttggaggcg atctttttcg tttttggtgc acaatcctcc atcgcttgct cggcgatagg
      421 taagaatgag cgggagatcg accgtcgagg gaaaggccga tgccactttc aattcatctt
      481 cgtcgaggtg atcgagccgc agttcggcgg cgccgatcca tgagcggttt ttctccacca
      541 atgctttgtt ttcagccaat gtcgaagcgg tgagcgttaa gcaaagcaag gaaaacctcc
      601 tgtggattgt caccgcaagc gcgggtgtgg tagactccat catagcgcct acagcagggc
      661 taatcaacac ccgcttggcc aagggagagg gagtttggct accttacagg tacagcggtt
      721 atccattgca ttcgccgatc gacagctgtt gaatgatatt tcttttacgc tttccgagcg
      781 ggctcgaacc gctctggtcg gggcgaacgg cagcggtaaa tccaccctct tgcaaatcat
      841 tgcaggattg gtgagcgcgg atatctacga atgcgcccac ccgaaagaga tgcggatctc
      901 gtaccttccg caaagcgata tcgtcctgga ggatggaacg gcgtttgaac aggtcgagct
      961 tgcctataat cgctttcttc cgcttgtcga tgaacagcac tcgattgaag agcgtcttgc
     1021 aacgggtgag ctttccgatt atctccttca gcggcttcat gagatccagg agactctcct
     1081 tgatgaggga tatcatgacc gaaagacgga gatcgcgatc attctcaagg gcctcgggtt
     1141 ccatgacgac gatcttcatc gtccttgcag cgagttttcc ggcggctggc agatgcgcat
     1201 cgcacttgca aaagtcttgg cggagaaacc ccatctcctc ctcttggacg aaccgaccaa
     1261 ctacctcgac attgaagccc gctactggct gagaaactac ctgagggtct atcccgagtc
     1321 ggtgatgatc gtcagccacg accgctcctt tctggatgag acggtcaacg aggtgtttga
     1381 actctttcac ggtcaattga agcgctataa gggaacgtac agccaatatg aagagcagcg
     1441 ggagcttgag attcaaagcc tcatccatgc ctatgaacaa cagcaggagg agatcaaaaa
     1501 gcagaaggag ttcatcgagc gcttccgggc gaaagcatcc aaagcccgcc aggtccaagg
     1561 gcgaataaaa cagctggaga agctggaaat cattgaaatt cccgagcacc tgagaaatct
     1621 ttccttctcc ttcccccccg ctccgcacag cggcaagcag gtattgaaga ttgaaaacct
     1681 caccaagcgc tacggttcgc ttaccgtctt cgaggggttc gactcgatca tcaccaaagg
     1741 ggaacggatc gccgtcgtcg gaaagaacgg aacggggaaa tcgactcttt tgagaatcct
     1801 tgcagggatc gacaccgatt accagggaag cctgaccgtc ggatcggggg ttcggatcgg
     1861 ctacttcgcc caggacgcga agcaggccct cgatgattcg aacacggtgc ttgatgagct
     1921 tgcaagcgtc gcatcgaccg ccgacattcc c
//
LOCUS       DAOPUZ010000251         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_63825_length_1947_cov_2.446089, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000251 DAOPUZ010000000
VERSION     DAOPUZ010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63825_length_1947_cov_2.446089"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..>1947
                     /locus_tag="PKN79_04725"
     CDS             62..>1947
                     /locus_tag="PKN79_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNZ95100.1"
                     /translation="MLKKILASIFVLLFTIGISAKDLMLADLPIRYGKERFVERIEER
                     TGGIREPIGLVLSGGSARAFAHIGVLRYLEEIGIVPDFIVSNSMGSIVGLLYAAGLSP
                     DQILEVTSSTSLETLFDLALPLKGGLLDSSRFLERISSILGRDLRLEDLEIPIIVTAE
                     DLVTKRQIQISEGDFYTVLVASFALPVYFPPVSYLGHRLIDGGITNLVPVGIAGEFTD
                     SIILSTTFYENEELNLNNPVTILNVTIDISKRRRGIEEMKALEDSFIWIRNDVESISF
                     MEFAKVLYLQEKGYEAAQANGEALGRLKRGISVSSFAEKRERLDEAIRAADRQYAAFE
                     HLRRFDDPQLLGIGLWSTLAGEVRPYLLDDFTIGISYTASRQALSFELNGGFAFNWAR
                     SGQMRTVPAIKTRLYYYFLDHWRVAFGGGVFYDARKEGFLFSYDGLLEGRYSLGRIAD
                     LRISQTFEGLFNDRNSRYVEAFPGSTCLATSTVTLELPSLKWLEKGSVDLAYQAYGDY
                     QRVRSFLNPYGSVKIRDPFIGIYLEGKTSVRFALDGKGDVPLFISDRFRTNHDPLITQ
                     GHDLTVSSNPSKYLAVVNLGFGYQPVSYRPTFGEMFLFEDSRVGVFADLLFNGDFFFS
                     VGFEA"
BASE COUNT          390 a          521 c          568 g          468 t
ORIGIN      
        1 catcaattgt ttgttgttat ttggatcttg atggggtaag gtttcctcac ggaggagata
       61 catgctcaag aagattcttg caagcatctt tgttttacta tttaccatcg gtatctctgc
      121 gaaagacttg atgcttgccg atctgccgat ccggtatggg aaggagcggt tcgtcgagcg
      181 gatcgaagag cgaaccggcg ggattcgcga accgatcggt ctggtgctca gcggcggttc
      241 ggctcgggcg ttcgcccata tcggcgttct ccggtatctt gaggagatcg ggattgtgcc
      301 cgatttcatc gtctccaact cgatgggctc gatcgtgggc ctgctctatg ccgccggcct
      361 ttctcccgat caaatcttgg aggttacttc ctcgaccagt ctggaaacgc tcttcgatct
      421 ggcgctcccc ttgaagggcg gcttgctcga ctcttcccgc tttctggagc ggatctcgtc
      481 gatcctcgga cgcgacctcc gtctggaaga tcttgagatc cccatcattg tgaccgctga
      541 ggatcttgta acgaaacgcc agatccagat cagcgagggg gacttctaca ccgtgctggt
      601 cgcctccttc gcgcttccgg tctactttcc cccggtctcc tacctagggc atcgcctgat
      661 cgacggggga atcaccaatc tggttcccgt gggaatcgcc ggtgaattca ccgattcgat
      721 catcctctcc acgaccttct acgaaaatga ggagctgaac ctgaacaacc cggtgacgat
      781 tctgaacgtc acgatcgaca tctccaagcg acggagggga atagaggaga tgaaggcgct
      841 ggaggattcg ttcatctgga tccgaaacga cgtcgagtcg atctccttca tggagtttgc
      901 caaagttctg tatcttcagg agaaggggta tgaagcggca caggcgaacg gcgaagcact
      961 ggggcggctg aagaggggaa tttccgtatc ctcgtttgcc gaaaaaagag aacggcttga
     1021 tgaggcgatc cgggccgccg atcggcaata cgccgccttt gaacacctta ggcggtttga
     1081 tgatcctcag ctgctgggaa tcggactgtg gtccaccctc gccggcgagg tgcggccata
     1141 cctcctcgat gatttcacca taggaatctc ctatacggcg tccaggcagg cgttgagctt
     1201 cgagctgaac gggggctttg cgttcaactg ggcccgaagt ggacagatgc gcaccgtccc
     1261 cgcgatcaag actcgcttgt actactactt tctcgaccat tggcgggtcg ctttcggggg
     1321 cggggtcttc tatgacgcga ggaaagaggg attcctgttt tcctacgatg ggctgcttga
     1381 ggggcggtat tcgcttggca ggatcgccga tctgaggata agccagacgt tcgaagggct
     1441 cttcaacgac aggaacagca ggtacgtgga ggcgttccct ggatcgacct gtctggcgac
     1501 gagcaccgtc acgctcgaac tgccaagctt aaaatggctg gagaaaggct cggtggatct
     1561 cgcctatcaa gcgtatgggg attatcaacg ggttcgaagc tttctcaacc cgtacgggag
     1621 tgtgaaaatc cgcgatccgt ttatcggaat ctatcttgaa gggaagacca gcgtccggtt
     1681 cgcgttggat ggcaagggcg atgtaccgct cttcatctcc gatcggtttc gaacgaacca
     1741 cgatccgctc ataacccaag ggcacgatct taccgtttca agcaatcctt cgaagtatct
     1801 ggccgtggtg aacctcgggt ttgggtatca gccggtgtcc taccggccga ccttcggcga
     1861 gatgttcctc ttcgaggaca gcagagtagg agtatttgcc gacctgctct tcaacggtga
     1921 tttcttcttc tcggtgggct tcgaggc
//
LOCUS       DAOPUZ010000252         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_63866_length_1946_cov_2.429402, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000252 DAOPUZ010000000
VERSION     DAOPUZ010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63866_length_1946_cov_2.429402"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PKN79_04730"
     CDS             complement(<1..171)
                     /locus_tag="PKN79_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase"
                     /protein_id="HNZ95101.1"
                     /translation="MEERIGLIIRGINNIYTVESGEEQYLCRIKGKQLEGVEGEYNPL
                     AVGDRVSFVVTGT"
     gene            235..792
                     /locus_tag="PKN79_04735"
     CDS             235..792
                     /locus_tag="PKN79_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNZ95102.1"
                     /translation="MYSFTRCKVSSCPRYATHISEYCLAHDPRQDLAPTLSFPVLDSA
                     SLSNWNCTEEDFSGKRILGSLFSYSTFHGVSFVKTTILNSNFSFCLFEECVFDESTIR
                     YVIFSGSTFTQCMFMNSSITHTNFNGSVMRDCDASGSDLYYSSFAHAHIHNTKMEDCN
                     VRKADFRHTLQDNLSFRWSNYREVL"
     gene            792..1337
                     /locus_tag="PKN79_04740"
     CDS             792..1337
                     /locus_tag="PKN79_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ95103.1"
                     /translation="MRIHQHFSVVGFCNTYVIGDNATKDALLIDPGHADIELIRLLEQ
                     HGYTIKAVLLTHRHSGHTEGLGLLKKLYDFTTYAYEDPRHPIIPDEILTIGPFEIFSY
                     LVGGHSRDSVVYKIKDALFVGDALRAAALGKTHTHRLAEHLKDELNRIFSSLDPQTLI
                     FPGHGPPSTIQSELLFNQSLR"
     gene            complement(1322..>1946)
                     /locus_tag="PKN79_04745"
     CDS             complement(1322..>1946)
                     /locus_tag="PKN79_04745"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNZ95104.1"
                     /translation="QGLVVHPAAGNWDNTLANGLLHRYGERILSVQDEGSAGVRPGIV
                     HRLDKETSGLMVIALTQAAHRALGAQFKDRSTSKIYIALVAGKVKNAKGTIDLHIRRD
                     PSNRKKMAVCSDDTGRQALTEYTVLRQFRDCALLYLVLHTGRTHQIRVHLSSIGHPIV
                     GDPLYGREVEEGMMLHAKSLAFTHPVTGERMTFTAPLPRRFFDYLKL"
BASE COUNT          419 a          572 c          477 g          478 t
ORIGIN      
        1 cgttcccgta acgacgaagc tgactcgatc gccgacggca agggggttgt attcgccctc
       61 aaccccttcc agctgctttc ccttgatgcg gcagagatac tgctcttccc cgctctctac
      121 ggtataaata ttattgattc ctcgaatgat cagtccaata cgttcttcca taccttcact
      181 gtacaacctt ctcttcaccc ttgacaacct tccatttctt gatagggtgt gatcatgtat
      241 tccttcaccc gctgcaaagt gagctcctgc ccccgttatg cgacccacat cagcgagtat
      301 tgtctggctc acgatccaag gcaggatctc gcgcccaccc tctcttttcc cgttctcgac
      361 agtgcgagtc tgagcaattg gaactgcacc gaggaagatt tttccgggaa acgaattctg
      421 ggctcgcttt tcagctactc gacctttcac ggggtttcat ttgtcaagac gaccattctg
      481 aactccaatt tctcgttttg cctctttgag gagtgcgtct ttgatgaaag cacgatccgc
      541 tatgtgatct tcagcggctc cacgttcacg cagtgcatgt ttatgaactc ttccatcacc
      601 cataccaact tcaacggatc ggtcatgagg gactgcgacg caagcggcag cgatctgtac
      661 tattcctcgt ttgcccacgc ccatatccac aacacgaaga tggaggattg caacgttcgg
      721 aaggcggatt ttcgtcatac cctccaggac aacctctcct tccgctggtc caattaccgg
      781 gaggtgctgt gatgaggatc caccagcact tctccgtcgt cggattctgc aacacctacg
      841 taatcgggga caatgccaca aaggatgctc ttctcatcga tcccggtcat gcggatattg
      901 aattgatccg gctcttagag cagcatggct atacgatcaa ggccgtcctt ctcacccatc
      961 gccacagcgg acacaccgaa gggttggggc ttttgaaaaa gctctatgat ttcactacgt
     1021 atgcgtatga agatccccgc catccgatca tccccgatga aatcctgaca atcggtccgt
     1081 ttgaaatctt ttcttacctg gtcggagggc acagcagaga ttcggttgtg tataaaatca
     1141 aagatgctct gttcgtgggc gatgcgctca gggcggcggc cctcggaaaa acccataccc
     1201 accgccttgc ggaacacctt aaggatgaat tgaatcggat cttctcctcc ctcgacccgc
     1261 aaaccctcat cttccccggc cacggtccgc caagcacgat ccaaagcgaa ttgttgttta
     1321 atcagagctt gagatagtcg aaaaaccttc gcggcagcgg tgcggtaaac gtcattcgtt
     1381 ctcccgtcac cggatgtgta aaggcgaggc ttttggcatg gagcatcatc ccctcttcga
     1441 cttcccggcc gtaaagggga tcacccacga tcggatgacc gatcgaggaa aggtgcaccc
     1501 tgatctgatg ggttcgtccg gtatgcagga cgagatacaa gagcgcgcaa tcgcggaact
     1561 ggcgaaggac ggtgtattcg gtcaatgcct gacgccccgt atcgtcggaa cacaccgcca
     1621 ttttctttcg attgcttgga tcccgccgga tatgcagatc gatggtccct ttggcattct
     1681 tcactttccc ggcaaccaag gcgatgtaga tcttgctcgt cgatcgatcc ttgaactggg
     1741 cgccgagcgc ccgatgggcg gcttgcgtaa gggcgatgac catcaacccg ctggtttcct
     1801 tgtcgaggcg atggacgatt cccggacgaa cgccggcgct gccttcatcc tgaacggaga
     1861 gaatccgttc gccatagcga tggagcaatc cgttggccag cgtattgtcc caatttccgg
     1921 cggcggggtg tacgacaagc ccctgc
//
LOCUS       DAOPUZ010000253         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_64128_length_1941_cov_2.527041, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000253 DAOPUZ010000000
VERSION     DAOPUZ010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64128_length_1941_cov_2.527041"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..564)
                     /locus_tag="PKN79_04750"
     CDS             complement(22..564)
                     /locus_tag="PKN79_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNZ95105.1"
                     /translation="MKTVLVLLAQGFEEIEAITPIDLLRRAGAKVTVATLKEDRAAGA
                     HGITVLSDTTLEKLGDRLFDCVVLPGGGEGSENLAASAAVLTKVIQIAQEGVVAAICA
                     APAVVLGKTGLLDGRKVTGYPGTAELVEGLVFEDDGVVVDGNLITAKGPAFAMDFALA
                     IIKKLFGGEQERKIRLGILR"
     gene            complement(574..1641)
                     /locus_tag="PKN79_04755"
     CDS             complement(574..1641)
                     /locus_tag="PKN79_04755"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNZ95106.1"
                     /translation="MANVDLRIQNIEAMASPAEVFARFPLDKDGSKAIASYRQTIAKI
                     IRGEDSRLLAIVGPCSIHDPKAALEYAQKLAVFAKEVEDQLFVVMRTYFEKPRTVGGW
                     KGLILDPDMDGSYDIEGGISLARSLLVEITHMGLPVGCEVLDPIIVQYIDDLISWSSI
                     GARTTESQVHRNLASGLSVPVGFKNSTSGELINAVYAVKSARSNASFIGMDKNGVSKI
                     FRTTGNELGHLILRGGDDGPNYYEDDVEHARDLMEKEGLLPAIVVDCSHANSRKDHHR
                     QKRVLRSIVDQVRWGERSIRGFMLESNLFEGCQRIGEHPADLIYGVSVTDPCIGWDET
                     VRILRRAYGILKDRTQDVPEL"
     gene            complement(1674..>1941)
                     /locus_tag="PKN79_04760"
     CDS             complement(1674..>1941)
                     /locus_tag="PKN79_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNZ95107.1"
                     /translation="VIPEGGALVALIKLDIEAFAQKMALSVNEAKTEAARYINKLREE
                     VNKELNVFSRLSKVHLQEDDFERTPTQKIKRFLYLRKKDESSDA"
BASE COUNT          384 a          603 c          516 g          438 t
ORIGIN      
        1 cgaagccgat ggttttcacg attacctcag aattcctaag cgaatcttcc gttcttgctc
       61 gccgccgaaa agcttcttga tgatcgcaag agcgaagtcc atggcgaacg ccggaccctt
      121 ggcggtgatg aggttgccgt cgacgaccac accgtcatcc tcgaacacga ggccttcgac
      181 cagctcggcg gttccgggat aaccggtaac cttcctgccg tccaaaaggc cggtctttcc
      241 caagaccacc gccggggcgg cgcagatcgc cgcgacgacc ccctcctggg cgatttggat
      301 caccttcgta aggacggcgg cggaagctgc gaggttttcc gagccctctc ctccgccggg
      361 caggacgacg cagtcaaaga gccgatctcc caacttctcc agggtcgtgt cgctgagaac
      421 cgtgatgccg tgcgcgccgg cagcccgatc ctccttaagc gtcgcgacgg tcaccttcgc
      481 acccgcccgc ctcagcaggt cgatcggggt gatcgcctcg atctcttcaa agccctgagc
      541 caaaagaacc aataccgttt tcatcccttc ctcctataat tccggcacgt cctgagtgcg
      601 atctttgagg attccatagg cgcgccgcaa gattcgcacg gtttcatccc agccgatgca
      661 cggatcggtg acggagacac catagatgag atcggcgggg tgttcgccga tgcgctggca
      721 tccctcaaag agattcgatt cgagcatgaa gccccgaatg gatcgctcgc cccaccgaac
      781 ctgatcgacg atcgagcgaa gaacccgttt ttgacgatga tgatctttgc ggctgttggc
      841 gtgggagcaa tccaccacga tcgccggcag gagcccctcc ttctccataa gatcccgggc
      901 gtgttcgaca tcatcctcgt agtagttcgg cccgtcatcc cctccgcgaa ggatgagatg
      961 ccccagttca ttaccggtcg ttctgaaaat tttggaaacg ccatttttat ccataccgat
     1021 gaacgaggcg tttgagcggg cgctctttac cgcatagacc gcgttgatca gttcgccgct
     1081 ggtcgaattc ttgaatccga cgggaacgct cagtccgctt gccagatttc ggtgcacctg
     1141 gctttccgtc gtccgcgcac cgatcgacga ccagctgatc agatcgtcga tatactgaac
     1201 gatgatcggg tcgagcacct cgcagccgac gggaagaccc atgtgggtga tttccaccag
     1261 cagcgagcgg gccaggctga tccccccctc gatgtcgtag gatccgtcca tgtcgggatc
     1321 gaggatcagg cccttccagc ctccgacggt tcggggcttc tcaaaatagg tcctcatcac
     1381 gacgaacagc tggtcttcca cctctttggc gaagacggcg agcttctgtg catactccag
     1441 cgccgccttc ggatcgtgga tcgaacaggg gccgacgatt gccagcagcc tcgaatcttc
     1501 accgcggatg atctttgcga tcgtctgtcg atacgaagcg atcgccttgc tgccgtcttt
     1561 atcaagggga aagcgggcga acacctcggc aggactcgcc atcgcctcta tgttttgaat
     1621 tcgaaggtct acgttagcca tacgcttggt atagcatatc cgctgctttc tcctcaagca
     1681 tcggaactct catctttttt tcggaggtat aaaaaccgct tgatcttctg agtgggagtc
     1741 cgttcaaagt catcctcttg caggtggact ttgctgagac ggctgaagac attcagctcc
     1801 ttgttcactt cttcccgcaa cttgttgata tagcgcgccg cttcggtctt cgcttcattc
     1861 acgctcaagg ccatcttctg tgcgaacgct tcgatatcca gcttgatgag agcgacgagc
     1921 gctccgccct cgggaatcac c
//
LOCUS       DAOPUZ010000254         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_64557_length_1932_cov_3.234949, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000254 DAOPUZ010000000
VERSION     DAOPUZ010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64557_length_1932_cov_3.234949"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PKN79_04765"
     CDS             <1..814
                     /locus_tag="PKN79_04765"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNZ95108.1"
                     /translation="GALGAGVGAGTLVKIVGTSSCDLTVAPLTSHFSDTKGLGGVVEH
                     SVLPGMWGIEAGQSGVGDIFDWYVNTFLGTTHQALMDEAATLRPGQSGLLSLDWHHGN
                     RSILQDQNLSGLILGLDLSTTPAEVYRALIEATAFGSKVILDRLQACSVQLEKVVLGG
                     GIPMKNPLLVQIYADVHERPMHLAECEYATALGSAIASSVAAGIHPSIEAAQKAIVPP
                     STRVVHPNEKATAVYRRLYPIYRTLHDGFGVADAAVDLYPVMKELLALKSGG"
     gene            complement(798..>1932)
                     /locus_tag="PKN79_04770"
     CDS             complement(798..>1932)
                     /locus_tag="PKN79_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNZ95109.1"
                     /translation="TDGLIEEVSALLGGKERVFEETGIAFQPFNTIYQLVAHKRDRGE
                     VFAAAKHYLALPDLLAYWLTGVMKNERSHASTTQLYNPKTRDWAWPIIDELGFDRSLF
                     GEIVDSGTVLGPLTVDLQHEVGASDKVVVIASAAHDTASAVAAVPAIAGQVPLYISSG
                     TWSLLGSELGEPLTTVEAMESGFTNEVAANSQIRYLKNIMGMWIQQECVRHWESQEGK
                     LTWKELDEQTLLEEAYQGSIDVNDLRFLKPNTYDNLMVDRIDAYLEEHGMEKPKNKGQ
                     YMVAIYRGLATAYAEAIGDLERVLGVSFASLNIIGGGSKNEILNQWAADATGLTVLAG
                     PVEATALGNLIVQSWATGELASLQEGRDLIRTLHKVKTFTPRS"
BASE COUNT          342 a          616 c          580 g          394 t
ORIGIN      
        1 cggagcgctc ggggcggggg taggcgccgg aacgctggtg aagatcgtgg ggacttcctc
       61 ctgcgatctg acggttgctc ccctgacgag ccacttctcc gataccaagg gtcttggcgg
      121 agtggtcgag cactccgtcc ttccggggat gtggggaatc gaagcgggcc aaagcggggt
      181 tggagacatc ttcgactggt atgtgaacac cttcctcgga accacccatc aagcgctcat
      241 ggatgaggcg gcaacgctga gacccggtca aagcggtctg ttgagccttg attggcacca
      301 tgggaaccgc tcgatcctgc aggaccagaa cctgagcggc ctgatcctcg gcctggacct
      361 gtcgaccact cccgccgaag tctaccgcgc gctcatcgaa gcgaccgcat tcggctccaa
      421 ggtgatcctt gatcggctac aagcgtgcag cgtgcagctt gaaaaggtcg ttcttggcgg
      481 cggcattccg atgaaaaacc cccttctggt tcagatctat gcggatgtcc atgagcggcc
      541 gatgcatctt gccgaatgtg aatacgcaac cgccctcggc agcgcgatcg catcaagcgt
      601 cgctgcgggc attcatccct cgatcgaagc cgcccaaaag gcgatcgtcc ccccatcgac
      661 ccgggttgtc catccgaatg agaaagcgac ggcggtctac cgtcgcctct atccgatcta
      721 tcgaacgctc catgacggct tcggcgttgc agatgccgcc gtcgacctgt atccggtgat
      781 gaaggagctc cttgccctca agagcggggg gtgaacgtct tcaccttgtg gagggttcta
      841 atcaggtcgc gcccttcctg cagggaggca agctcgccgg tggcccaact ctggacgatc
      901 aggtttccca gcgctgtcgc ctcgaccggg ccggcaagca ccgtcaagcc ggtggcatcg
      961 gcggcccact ggttcagaat ctcgttcttc gatcctccgc cgatgatgtt cagcgaggca
     1021 aacgagactc cgagcacccg ctcaagatcc ccgatcgcct cggcgtacgc tgtggcgagc
     1081 ccccggtaga tggcgaccat gtactggcct ttgttcttcg gcttctccat cccgtgctct
     1141 tcgaggtagg catcgatccg gtcaaccatc agattgtcgt aggtgttggg cttgagaaat
     1201 cgaaggtcgt tgacatcgat cgaaccttga taggcttcct ctagcagggt ctgttcatcc
     1261 agttccttcc aggtcagctt cccctcctgg ctttcccagt ggcgaacgca ctcctgttgg
     1321 atccacattc ccatgatgtt cttgaggtag cggatctgcg agttggcggc aacctcgttg
     1381 gtgaagccgc tttccatcgc ctcgacggtg gtcagcggct cgccgagctc gctgcccaag
     1441 agcgaccacg tcccgctgga gatgtagagg ggcacctgtc ccgcgatcgc gggaaccgcc
     1501 gccaccgcgc tcgccgtgtc gtgggcggcg gaggcgatca ccaccacctt atccgaagct
     1561 ccgacctcgt gttggaggtc caccgtcaag gggccgagca ccgtcccgct gtcgacgatc
     1621 tccccgaaga gggagcggtc gaagccgagc tcatcgatga tcggccacgc ccagtctcgg
     1681 gttttcgggt tgtacagctg ggtggtcgaa gcgtgggagc gctcattctt catcactccg
     1741 gtcagccagt aggcaagcag atccggcagg gccagatagt gcttcgccgc ggcgaacacc
     1801 tccccccggt cccgcttgtg cgccacgagc tgatagatcg tgttgaacgg ctggaaggcg
     1861 atcccggtct cctcgaagac ccgctccttt cctcccagaa gcgcgctcac ttcctcgatc
     1921 aggccgtcgg tt
//
LOCUS       DAOPUZ010000255         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_64927_length_1925_cov_3.798930, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000255 DAOPUZ010000000
VERSION     DAOPUZ010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64927_length_1925_cov_3.798930"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PKN79_04775"
     CDS             <1..304
                     /locus_tag="PKN79_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNZ95110.1"
                     /translation="GYIWGIVGMFIAVPLTSVMQILFANIKSLRPFAIMISSGKSYQR
                     ERARQKALKSYQRRTQHRKSEQPAGEQGNESSEDSMAKESVNMGDFVLPEQFGEKK"
     gene            301..1167
                     /locus_tag="PKN79_04780"
     CDS             301..1167
                     /locus_tag="PKN79_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HNZ95111.1"
                     /translation="MIEFPSLMEEDARRYLLSSNPSYDVLIETVLTFYRDWGRRFVFR
                     DRHDPYSVLLGEVMLQQTQTSRVVDKYRYFLAEWPSFKALARAPLEEILIAWKGLGYN
                     RRALNLKKAAEMTEAWNWTVPNDYKVMRSLPGVGKATAAALLAFCYQEKSIYLETNIR
                     RVLLHYYFPDQTDVHDRALEAILSELIVLIDDPRSWYYALMDYGVLLKALVPNPNTKS
                     AHYAKQSRFEGSKRQVRAALLHCVAEQGAIALSSVTEILKEYDSTYLAQSIEELIKEG
                     FLLLEKDSLSIR"
     gene            complement(1118..>1925)
                     /locus_tag="PKN79_04785"
     CDS             complement(1118..>1925)
                     /locus_tag="PKN79_04785"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ95112.1"
                     /translation="TLVSTILFFGRNNPAIAGPKQRAPWPLYLNGMLGVLILNFNFHS
                     FTALGSSLAMALTVFGQSVLSLVFDLNGWLGMKKRSFSPKKVLSLAVSGLGIVVMASK
                     GVGSFSWIYILIAFAAGAITITQMILNSTFAIYKGPMKASQWTYIAGMSAAVPFYFLT
                     RPSATIEGFARLPGVPFIQIIAGGVLSVTVLISTAFVIVKLPAIYSALLLSASQILAS
                     VAIDAFFLKIFSPNLLFGALLMLLGMAGNLLADRSTGSKENPSQEGETLP"
BASE COUNT          461 a          502 c          569 g          393 t
ORIGIN      
        1 ggggtatatc tgggggatcg tcgggatgtt catcgccgtt ccgctgacca gcgtgatgca
       61 gattcttttc gccaatatca agagcctgag gccctttgcg atcatgatca gcagtggaaa
      121 aagctaccaa cgggagcggg cccgccagaa agcccttaag agctatcaac ggcggaccca
      181 gcaccggaaa tctgaacagc ctgccggcga gcagggcaat gaatcgagcg aggactcgat
      241 ggcgaaagaa tccgtcaata tgggtgactt tgtccttccg gagcagtttg gcgaaaagaa
      301 atgattgaat ttccttcgct tatggaagag gatgcccgaa gatatctcct gtcatccaac
      361 ccctcttatg atgtattgat tgaaacagtc ttgaccttct accgggattg gggacgccgg
      421 ttcgtcttcc gcgatcgtca cgatccgtat tcggtgctcc ttggcgaggt gatgcttcag
      481 cagacccaaa catcccgggt ggtcgacaaa taccggtatt tccttgccga atggccctct
      541 ttcaaagccc tcgcccgcgc tccgctagag gagatcctca tcgcttggaa gggcctcgga
      601 tacaaccggc gggcgttgaa tctgaagaaa gccgccgaga tgaccgaagc atggaattgg
      661 accgttccca atgactacaa ggtgatgcgc tcgctccccg gcgtgggaaa agccaccgca
      721 gcagccctct tggccttctg ctaccaagaa aaatcaatct atctggaaac gaatattcga
      781 cgggttctgc tgcattacta tttccccgat cagaccgatg tccatgaccg ggcgttggaa
      841 gcgatcctca gtgagctcat tgtgctcatt gatgatcccc gttcctggta ctacgcgctg
      901 atggattacg gggtgctgct caaagccctg gttccgaatc ccaacaccaa aagcgcccac
      961 tatgcgaaac aaagccggtt tgaagggtcc aagcgccaag tccgggcggc acttcttcat
     1021 tgtgtcgccg aacagggggc gatcgctctt tcatccgtta ccgagatctt gaaagagtat
     1081 gattcaacat accttgctca atctattgag gagctcatca aggaagggtt tctccttctt
     1141 gagaaggatt ctctttcgat ccggtagaac gatcggcaag caggttgccc gccatcccca
     1201 gaagcatcag gagcgctccg aagaggaggt tgggggagaa gatcttcagg aagaacgcat
     1261 cgatcgccac cgatgcgaga atctgactcg ccgagagaag cagggcggag tagatcgccg
     1321 ggagcttcac gatgacgaac gccgtggaga tcaggacggt caccgagagg acgccgcctg
     1381 cgatgatttg gatgaacggc acgcccggca atctggcgaa tccttcgatc gtcgccgaag
     1441 gccgagtgag aaagtagaag gggaccgcgg cgctcatgcc ggcaatgtag gtccattggc
     1501 tggctttcat cgggccttta tagattgcga aggtggagtt gaggatcatc tgggtgatcg
     1561 tgatcgcacc ggcggcgaag gcgatgagga tgtagatcca tgaaaacgag ccgacgccct
     1621 tggaggccat cacgacgatt cccaggccgc tgaccgccag gctcagcact ttcttcgggg
     1681 agaaagagcg cttcttcatc cccaaccatc cgttgaggtc aaatacaagg gaaagaaccg
     1741 attgaccgaa gacggtaagc gccatcgcca aggaggaacc gagggcggtg aaggaatgaa
     1801 agttgaagtt gagaataagg acgccgagca tcccgttgag gtagagaggc cagggggctc
     1861 gttgtttcgg accggcgatc gcggggttgt tccgcccgaa gaagaggatc gtcgagacga
     1921 gggtg
//
LOCUS       DAOPUZ010000256         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_65201_length_1919_cov_2.835837, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000256 DAOPUZ010000000
VERSION     DAOPUZ010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65201_length_1919_cov_2.835837"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKN79_04790"
     CDS             complement(<1..451)
                     /locus_tag="PKN79_04790"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNZ95113.1"
                     /translation="MSDAIIGFHAIEEGLKSASGGSVLYIAREMCSKAEVLENLARMN
                     RKTIVRKVTGTELDRLAGSSNHRGALLVLGSRSKSSSASRQLTVQEFCSNLKEEEGAL
                     VLILDGITDPQNLGAILRSADQFSVSLVIIPSRRSAQSNQTVVKVSSG"
     gene            complement(454..1578)
                     /gene="dnaJ"
                     /locus_tag="PKN79_04795"
     CDS             complement(454..1578)
                     /gene="dnaJ"
                     /locus_tag="PKN79_04795"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNZ95114.1"
                     /translation="MAKRDYYEVLGVEKTATLDEIKKAYRKLAIANHPDRNQGDKAAE
                     DRFKEATEAYDVLSDEKKRQMYDQFGFAGVDGGSGPRDYSNVYRDFSDIFGSGFGGGF
                     EDIFSSFFGGGGRASRAHRGPEPGSSLRYDIEIDFKDAIFGTKVEISYSHQVACDVCG
                     GSGSESSAGMKTCPTCHGSGQVSRGSGFFTIASTCPTCSGNGQIIENPCQGCHGTGLK
                     RKQQKVKVTVPAGVDTGSRVVLRGMGDAGPNGGASGDLYVYINVRPHKYFIRQGDNLF
                     VQIPISITQAALGAEIMVPTIDGKKIKLSIPSGTQHGKMLRLRQYGATRLNSTDRGDM
                     YVKIDIQVPKRLNSKAKRLMQELSDALGEVTEPNPVSFED"
     gene            complement(1657..>1919)
                     /gene="dnaK"
                     /locus_tag="PKN79_04800"
     CDS             complement(1657..>1919)
                     /gene="dnaK"
                     /locus_tag="PKN79_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709838.1"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNZ95115.1"
                     /translation="DKGSAANRSRIEKAVAHLKKDMEGSDVEAIKAKTEALKQASYKL
                     AEEMYKQTQAEAGGPQTETESTAGSSSDTVKNAEDADFEVVD"
BASE COUNT          384 a          561 c          506 g          468 t
ORIGIN      
        1 cccccgagga gactttgacc accgtctggt tggattgggc gctccgcctg cttgggatga
       61 tgacaaggga gacggaaaac tggtcggcgg agcggaggat cgccccgaga ttttgggggt
      121 ccgtgatccc gtcgaggatc agcacgagag caccttcctc ttccttcagg ttcgagcaga
      181 actcctgaac cgtcagctga cgggaagcgg atgagctttt acttcgggaa ccgaggacaa
      241 gaagagctcc ccgatgattg gaggagccgg cgagtcgatc gagttcggtt ccggttacct
      301 tccgaacgat cgtttttcga ttcatccgcg ccagattttc cagcacctcg gctttcgagc
      361 acatctcccg ggcgatatag aggacggagc cgccggaggc gcttttaagt ccctcttcga
      421 tcgcgtgaaa gccgataatc gcatcgctca tcatcaatcc tcaaaagaaa ccggattcgg
      481 ttcggtgacc tcgccgaggg cgtcactgag ctcctgcatc agtctttttg ctttagaatt
      541 cagccgttta ggaacttgga tatcaatttt cacgtacata tccccccgat ccgtcgaatt
      601 gagccgggtg gcgccgtact ggcggagacg gagcatcttc ccgtgctggg ttcccgacgg
      661 gatgctgagc ttgatcttct tgccgtcgat cgtcggcacc atgatctccg ctccgagagc
      721 ggcctgagtg attgagatcg ggatctggac gaagagattg tctccctgac ggatgaagta
      781 cttgtgcggt ctgacattga tgtagacgta cagatcgccg cttgctcctc cgttgggtcc
      841 ggcgtccccc attccccgca gaaccaccct gctgccggta tcgactccgg cgggaacggt
      901 gaccttcacc ttctgttgct tgcgcttcaa gccggtgccg tgacacccct gacagggatt
      961 ctcgatgatc tgcccgtttc cgctgcaggt gggacaggtc gaagcgatcg tgaagaagcc
     1021 gctgcctctg cttacctgac cggagccgtg gcaggtggga caggttttca tccctgccga
     1081 actttcactc cccgaccccc cgcagacatc gcatgccact tggtgggagt aggagatttc
     1141 aactttcgtt ccaaaaatcg catctttaaa atcgatttca atgtcatagc gaagggaaga
     1201 gcccggttcg ggaccccgat gggctcttga ggctcgtccg cctcccccga agaaggagga
     1261 aaaaatatcc tcaaacccgc caccgaagcc ggaaccgaag atatcgctaa agtcacgata
     1321 gacgttggaa taatcacgcg ggcccgaacc gccatccact cctgcaaagc cgaattggtc
     1381 gtacatctgt cgcttcttct catccgagag aacatcatac gcctcggttg cttccttgaa
     1441 gcggtcctcg gccgccttgt caccctgatt gcgatcgggg tgattggcga tcgcaagttt
     1501 gcgatatgct tttttaattt catccaaggt agcggtcttt tccaccccaa ggacctcata
     1561 ataatctcgt ttcgccacag tttcctcgtc tacaccatca tgtcggccgg agccgacatg
     1621 atggtaatca acgtattcta tcgtccttca aggccgttag tctacgacct caaagtcggc
     1681 atcctcagca ttttttaccg tatccgaaga ggagccggcc gtactttccg tctcggtctg
     1741 agggcctccg gcttccgctt gggtctgttt gtacatctct tcagccagtt tataagaagc
     1801 ctgcttgaga gcctcagtct ttgccttaat agcttcgaca tcgctgcctt ccatgtcttt
     1861 cttaagatgc gccaccgctt tttcgatccg ggaacggtta gcagcgctcc ccttgtcac
//
LOCUS       DAOPUZ010000257         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_65417_length_1915_cov_3.149462, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000257 DAOPUZ010000000
VERSION     DAOPUZ010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65417_length_1915_cov_3.149462"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /gene="uvrC"
                     /locus_tag="PKN79_04805"
     CDS             complement(<1..1264)
                     /gene="uvrC"
                     /locus_tag="PKN79_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNZ95116.1"
                     /translation="MAKKETRYTVGSEIETFNTVVSEPVAPSLQARELPGQSGVYLMK
                     DKDDRIIYVGKAKDLRKRVTSYFLANRSAKTAALVRKIVTIEYIITGNEYEALVLENN
                     LIKKYAPHYNISLKDGKSYPVIRITNEPFPKVLKTRQIINDGSTYYGPYPGARMLEQY
                     LDLIYKLFPLRYCSTPLRKREKPCLYYHIGLCSGPCAGLIGAEEYGRYITEVRNLLEG
                     KVDGLLTQLHEEMTKASRELRFEEAATKRDQAETVKMISTDQQVQEFSEENRDYAAIE
                     MRGPLCTISLMQMREGQVIGRALYRAETFGDETETLLAFLIQYYADGMKLPQYLYVSH
                     EIEVDLIRRYFLEHFGGILEVSLPIEGKHYRILRMARENATRDVEKRLRNKDNTEALH
                     LLKELLGLESEPVLIEGFDVSHLQGKYTV"
     gene            complement(1314..>1915)
                     /gene="thyX"
                     /locus_tag="PKN79_04810"
     CDS             complement(1314..>1915)
                     /gene="thyX"
                     /locus_tag="PKN79_04810"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNZ95117.1"
                     /translation="NFTFHIKMPIFVARQWVRHRTARVNEISGRYSVMKDEFYVPDSR
                     HINLQSEDNKQGRMDEAVDPALAKKVQEILAADQKRSYETYRQLLEMGLARELARINL
                     PLSMYTEWYWQMDLHNLFHFLKLRLDPHAQWEIRVYAEQILKIIRLVCPIAVEAFEQH
                     KVGSATFSASELEALKDVLDGKENPLTGRALQLFEEKLK"
BASE COUNT          360 a          538 c          540 g          477 t
ORIGIN      
        1 cgacggtgta tttgccctga aggtgggaaa cgtcgaagcc ttcgatcaaa acgggttcgc
       61 tctccagacc gaggagctct ttcaacagat ggagcgcttc ggtgttgtcc ttgttcctca
      121 gtcgtttttc gacatcccgc gtcgcgttct ctcgggccat ccgcaggata cgatagtgtt
      181 ttccctcgat gggaaggctc acctccaaga tgcccccgaa gtgctcaagg aagtagcgcc
      241 gaatcagatc gacctcgatc tcatgcgaga cgtagaggta ttggggaagc ttcatgccgt
      301 cggcataata ttggatgaga aacgccagaa gcgtctccgt ctcgtccccg aacgtctccg
      361 cccgatagag cgcccgtccg atcacctgcc cctcccgcat ctgcatcagg ctgatcgtgc
      421 agagcggacc gcgcatctcg atcgcggcgt agtcgcggtt ttcttcgctg aactcctgga
      481 cttgctgatc cgttgaaatc atcttcaccg tttccgcctg atcgcgcttc gtcgccgctt
      541 cttcaaagcg cagctcccta ctggctttgg tcatctcctc atgaagctga gtgagcaacc
      601 cgtcgacctt tccctccaga aggttccgaa cctcggtgat gtatctgcca tactcctccg
      661 cgccgatcaa gccggcgcat ggaccgctgc agagcccgat gtggtagtac agacagggtt
      721 tctcccgttt ccgcaaggga gtcgaacagt agcggagcgg aaagagcttg tagatcagat
      781 cgaggtattg ttccaacatc cgcgcacccg ggtaggggcc gtagtaagtt gacccgtcgt
      841 tgataatctg gcgggtttta aggactttgg gaaacggttc attggtgatt cgaatcaccg
      901 gataggattt tccgtctttc agggagatgt tgtagtgggg agcgtatttt ttgatgaggt
      961 tgttctccag gacgagcgcc tcatactcgt ttcccgtgat gatgtattcg atagtgacga
     1021 ttttgcggac caaggcggcg gtcttggcgc tccggttggc cagaaagtag ctggtcaccc
     1081 gcttgcgcaa atccttcgct ttgcccacgt agatgatccg atcatccttg tccttcatca
     1141 gatacacgcc gctctggccg ggcagctccc gagcctgaag gctcggagcg accggttcgc
     1201 tgacaaccgt gttgaaggtc tcgatctccg aacctacggt gtaccgtgtt tccttctttg
     1261 ccatggatta actgtaccca ctccccagtt ctttgacaat tgcgggggtt tcgctacttc
     1321 agtttctcct caaagagctg aagagcccgg ccggtcaggg ggttttcttt tccatcgagc
     1381 acgtctttga gggcttcaag ctcgcttgcg ctgaacgttg cgctgccgac cttgtgttgc
     1441 tcgaacgctt cgacggcgat cggacatacc aagcggatga tcttcaatat ctgttcggcg
     1501 tagacgcgga tctcccattg ggcgtgcgga tcgagccgaa gtttgaggaa gtggaagagg
     1561 ttgtgcagat ccatctgcca gtaccattcg gtgtacatcg agagcggaag gttgatccgg
     1621 gcgagctccc gggccaagcc catctccaga agctgccggt aggtctcata gcttcgcttc
     1681 tggtcggcgg caaggatctc ctggaccttt ttggccaagg cgggatcgac cgcttcatcc
     1741 atccggcctt gcttgttatc ctcgctctgg aggttgatgt gccggctgtc aggaacgtag
     1801 aactcatcct tcatcactga ataccggccg ctgatctcgt tgacccgggc ggttcggtgc
     1861 cggacccact gccgggcgac aaagatcggc atcttgatgt ggaaggtgaa gttca
//
LOCUS       DAOPUZ010000258         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_65487_length_1914_cov_3.102205, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000258 DAOPUZ010000000
VERSION     DAOPUZ010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65487_length_1914_cov_3.102205"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKN79_04815"
     CDS             <1..512
                     /locus_tag="PKN79_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ95118.1"
                     /translation="NSVFYELTYEGYAPGGVAIIIDTLTDNKNRTASEVRSTLTKGGG
                     TLGTTGSVSYMFQTKGMIIYDDQKYTEEEIFEAALDHGADDVLHEDGMIEVYTAPGDF
                     ANVLESLQASGFEEESAEIAKIADQTVELDDERTRKVLKILERLEELDDVQQVFSNLE
                     IPDGFEDEE"
     gene            517..1008
                     /locus_tag="PKN79_04820"
     CDS             517..1008
                     /locus_tag="PKN79_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNZ95119.1"
                     /translation="MRILGIDPGFAQTGWGVVETTRQHTRLVSYGVIRTTTKENDSSR
                     IHFIAKQVGLLAVEHGVEICGMEDIYFAKNISSAIPVAKVIGSIMHELTAREIPIRFF
                     SPPVIKSSVVGYGGADKTQVQEMVRILLWLEKIPRPDHAADALAAALCLQAHESTRIR
                     MNS"
     gene            1008..1628
                     /gene="ruvA"
                     /locus_tag="PKN79_04825"
     CDS             1008..1628
                     /gene="ruvA"
                     /locus_tag="PKN79_04825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNZ95120.1"
                     /translation="MINALIGELVSVKEGSMIVRSNNIEYVLLISNQSASRFSSLDKE
                     ERKLIKILTSLQVREDSLTLYGFYDDAEREAFEQLQTVSGIGPKQALKILSGINVRHL
                     AEALDSGNIKTLATIPGIGPKTAQKMVLALRNVLVLDEPKMKQTTSRTAVWADVVNAL
                     VEMGFERRRSEEVIGDLEIEFAREIGELSRQSAEELLFRHALKNLS"
     gene            1637..>1914
                     /locus_tag="PKN79_04830"
     CDS             1637..>1914
                     /locus_tag="PKN79_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ95121.1"
                     /translation="MDHYNQLLENSVVSASFQEIADRQENVLRPKMLSDFQGQARLKE
                     NLSIFIQAALQRGEALDHTFLIGPPGLGKTTLASIIANEMGSEIRMTSA"
BASE COUNT          508 a          475 c          528 g          403 t
ORIGIN      
        1 agaattcggt cttctacgaa ctcacttacg agggatacgc tcccggcggg gttgcgatca
       61 tcatcgatac ccttaccgat aataaaaacc gaaccgccag cgaggtccgc tcgacgctca
      121 ccaaaggcgg aggaacgctc ggtactaccg ggtccgtctc ctacatgttc caaacaaaag
      181 gaatgatcat ctacgacgat cagaaatata ccgaagaaga gatcttcgaa gcggcccttg
      241 accacggagc cgatgatgtg cttcatgaag acgggatgat cgaggtatac accgctcccg
      301 gcgactttgc caacgtcctt gaatcgctgc aggcatcggg ttttgaagaa gaaagcgccg
      361 agatcgcgaa gatcgccgat cagacggtcg agcttgatga tgaacgaact cggaaagtgc
      421 tgaaaatatt ggagcgtttg gaagagctgg acgatgtcca gcaggtattc tcgaaccttg
      481 agattcccga cggcttcgag gatgaagaat aaggggatgc gtattcttgg aatcgatccc
      541 ggattcgccc aaacaggctg gggggttgtc gaaacaacgc gacaacacac ccggcttgtt
      601 tcatatgggg taatccgaac gacaaccaaa gagaacgatt ccagtcgaat ccatttcatc
      661 gccaaacaag tcggcctgtt ggctgtcgag cacggcgttg agatctgcgg aatggaagat
      721 atctactttg ccaaaaacat ctccagcgcg atccccgtag cgaaggtgat cggatcgatc
      781 atgcacgaac tgaccgcccg agagattccg atccgcttct tcagtcctcc ggtgatcaaa
      841 agttcggtcg tcggctatgg aggagcggac aagacccagg tgcaggagat ggtccgaatt
      901 ttactttggc ttgaaaagat tccccgaccc gaccacgctg cggacgcctt ggccgcagcc
      961 ttgtgtctgc aagcccatga atcgactcga ataaggatga acagctgatg attaatgccc
     1021 tgataggtga gctagtctcc gttaaagaag gatctatgat cgttcggtca aacaatatcg
     1081 agtatgtcct gttgatctcc aaccaaagcg caagccgatt ctcaagcctt gataaggaag
     1141 aacgcaagct catcaagatt ttgacaagct tgcaggtgcg ggaagactcc ttgacgttgt
     1201 acgggtttta tgatgatgcg gaacgggaag cgttcgaaca gcttcaaacg gtcagtggaa
     1261 tagggccgaa gcaggcgttg aagattctca gtggaatcaa tgtccggcat ctggcggaag
     1321 cattggacag cggaaacatc aaaacgctcg ccacgattcc cggcatcggt ccaaaaaccg
     1381 cccaaaagat ggtccttgcg ttgcgcaatg tgctggtgct cgatgaaccg aagatgaaac
     1441 aaacgacctc tcggacagca gtatgggcgg atgttgtaaa cgcccttgtt gagatgggtt
     1501 ttgagcgccg ccgttcggaa gaagtcatcg gagatttgga aatcgaattc gccagagaga
     1561 tcggtgaatt gagtcgccaa agtgctgaag agctgttgtt ccgccatgcg ctgaagaacc
     1621 tgagctagga gaggatatgg atcattacaa ccaattgctc gaaaactcgg tagtttcagc
     1681 ctccttccag gagatcgccg accggcagga aaacgtcttg aggcccaaga tgctttcgga
     1741 tttccaaggc caggcacggc tcaaggagaa cctctccatt ttcattcaag ccgctctaca
     1801 gcggggcgaa gcgcttgatc atacgtttct gatcggaccg ccggggttgg ggaaaaccac
     1861 cttggcatcg atcatcgcca atgagatggg atcggagatc cgcatgacca gcgc
//
LOCUS       DAOPUZ010000259         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_65620_length_1912_cov_2.530425, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000259 DAOPUZ010000000
VERSION     DAOPUZ010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65620_length_1912_cov_2.530425"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PKN79_04835"
     CDS             complement(<1..251)
                     /locus_tag="PKN79_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95122.1"
                     /translation="MVEKQLQELIAGWDGSLQWRYAHLANAAALLWEETEGINWLGFY
                     LIDKETDKLILGPFQGKAACTEIPIGKGVLDAAGQRIVG"
     gene            complement(245..913)
                     /locus_tag="PKN79_04840"
     CDS             complement(245..913)
                     /locus_tag="PKN79_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNZ95123.1"
                     /translation="MESKRGIPIPTIKRFPSYLRLLNVFKEEGREMASATMLAEELNL
                     KPIQVRKDISFTGIEGKPKVGFEVDKLINAIISVLGWDNATDAIIIGAGHLGSALARY
                     EGFGNYGLRIVAAFDTAEEKWGTNVGDVPIHPFDHLHRYIEENHVNIAVLTVPADEAQ
                     HVADQLVALGIMAIWNFAPKDIKVPSRVVLQRTDLATSFAVLSAKVKRVMESREFNEE
                     DDSW"
     gene            complement(913..1761)
                     /gene="ispH"
                     /locus_tag="PKN79_04845"
     CDS             complement(913..1761)
                     /gene="ispH"
                     /locus_tag="PKN79_04845"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNZ95124.1"
                     /translation="MKIVLAKTMGYCGGVSRALDLAEEAIAYATEHALPVYSLGKLIH
                     NPQVIASLAERGMSIIEHSKGYEPGVLVVRAHGISDTLRKEFVDAGFLLFDATCPVVR
                     HNLSNIAHWAKTRPILIIGHKRHPDVLAMQGVHVDGKPIKSFLITNAEETSGLIDRPY
                     AVFVQTTFDQVLLEEIRGSLEAFHHVVYINEICPSSISRRMAALDLARQCDAALVIGG
                     KQSANTKALAELVSNEGKKAYHIEDSTEIPVEAFSYGTLGILAGASTPSSVIEEVVQT
                     LQRSKT"
BASE COUNT          423 a          552 c          493 g          444 t
ORIGIN      
        1 tcaccaacga tgcgctggcc cgccgcatcg agcaccccct ttccgatcgg aatctcggtg
       61 catgccgcct tcccctgaaa ggggccgagg atgagcttgt ctgtttcttt atcgatgagg
      121 tagaacccca gccagttgat tccttccgtc tcctcccaaa gcagggcagc ggcattggcg
      181 aggtgggcat accgccattg aagcgaaccg tcccatcccg caatcaactc ttgcaactgc
      241 ttttctacca cgaatcatcc tcttcattga actcgcggct ttccatcacc cgctttacct
      301 tggcggaaag aacggcgaat gaggtcgcca agtcggtgcg ctgaagaacg acccgggagg
      361 gaactttgat gtctttgggg gcgaagttcc agatcgccat gatccccaag gcgaccaatt
      421 gatcggctac gtgctgggct tcatccgccg gaacggtgag cacggcgata ttgacgtggt
      481 tttcttcgat ataccggtga aggtgatcga atggatggat cggaacatcc cctacattgg
      541 ttccccactt ctcctctgcg gtatcgaagg cggctacgat cctgaggccg tagtttccaa
      601 acccctcata gcgggcgaga gcggaaccaa ggtgccccgc cccgatgatg atcgcgtcgg
      661 tggcgttgtc ccacccgagg acggagatga tcgcattgat cagcttatcc acctcaaagc
      721 ctactttcgg tttgccctcg atgccggtga aggagatgtc cttgcggact tggatcggct
      781 tgagattcag ctcctcggca agcattgtcg cacttgccat ctccctgcct tcctctttga
      841 acacgttcag cagccgcaga taggagggaa accgcttgat cgtcgggatt ggaattcctc
      901 gcttgctttc cattacgtct tgctcctttg cagagtctgc accacctcct caataacact
      961 tgaaggggtg gaggcgccag ccagtatccc cagcgtaccg tatgaaaatg cctcaacggg
     1021 aatttccgtg ctgtcttcaa tatgatacgc tttttttccc tcatttgaaa ccaattccgc
     1081 aagcgctttg gtattggcgc tttgctttcc cccgatcacc agcgcggcat cgcactgtcg
     1141 cgcgaggtca agcgccgcca tccggcggct gatcgaagaa ggacagatct cgttgatgta
     1201 cacaacatga tgaaaggcct caagagagcc tcgaatctct tcaagcagca cttggtcaaa
     1261 cgtggtctgg acgaacaccg catatggtcg atcgatcaaa cctgacgtct cttccgcatt
     1321 tgtaatgagg aaacttttta tcggtttccc gtcgacatgc acaccctgca tcgccagcac
     1381 gtccggatgc cttttatgac cgatgatgag gatcggacgg gttttcgccc aatgggcgat
     1441 atttgaaagg ttatggcgca ccaccggaca cgtcgcatcg aaaagaagaa aaccggcatc
     1501 aacaaactct ttccgcaacg tatcgctgat tccatgggcg cggacgacca gcaccccggg
     1561 ttcatacccc ttagaatgtt cgatgatgct catcccgcgc tccgccaacg aagcgatgac
     1621 ttgagggttg tggatcagtt ttcccagcga atagacggga agggcgtgct ccgttgcata
     1681 ggcgatcgct tcttcggcaa gatccagcgc ccggctcacc ccgccgcaat accccatcgt
     1741 ctttgcaagt acgatcttca tgcattgagt atgtcaaaaa aaaagccgct cgataagggg
     1801 agcggctcga caaaatacaa aagcactact tggctttatc ctcgatctga acgttcttct
     1861 gcgccttgct gatccagtac agcaaggaag gcgcaaggag gttggaggag ta
//
LOCUS       DAOPUZ010000260         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_65695_length_1910_cov_2.718598, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000260 DAOPUZ010000000
VERSION     DAOPUZ010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65695_length_1910_cov_2.718598"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="PKN79_04850"
     CDS             <1..159
                     /locus_tag="PKN79_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HNZ95125.1"
                     /translation="EKIAKAGDPGFTGFTFVERFAFYEQSKKAYAIVATGESALYANI
                     ILKKGVVL"
     gene            complement(156..1043)
                     /locus_tag="PKN79_04855"
     CDS             complement(156..1043)
                     /locus_tag="PKN79_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ95126.1"
                     /translation="MSLVLRASDYFYDPSFPIKVLERDPEIPYPLHSHDFWELIVIVS
                     GSGTHFDTAGSFELTSGNVFVIPPGFKHGYRDVRDLRLFNILFDISMFEESFFDLSNM
                     SGYQALLKVEPSYRHDGSLSTLMQLTPAQQARVLPLLEHMLVESDSTESEIGAKAGAF
                     AILAQLLVMLFRFYAEQPRKDNQTVMRIADALSFLEAHPDRSVSTSELMELTNMSAST
                     LNRHFKNATGLSPIEFHIQRRIKHACRLLRSSDMSVSEISEATGFEDSNYFSRQFRNV
                     MGITPLSYRKNRSMWYRLP"
     gene            1128..>1910
                     /locus_tag="PKN79_04860"
     CDS             1128..>1910
                     /locus_tag="PKN79_04860"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HNZ95127.1"
                     /translation="METTSYQYAKERYRTIGVDTDAVLKRLDRIPISIHCWQGDDVGG
                     FEAPDAELSGGGIQVTGSYPGKAQTIEQLRADLEEVLSIVPGPHRISLHASYGEFGSE
                     FVDRNEIEPKHFQGWVDWAQANNVHLDFNGTFFSHPMANDGYTLASKDEEIRQFWIEH
                     AKRTRKIAAWIGKQQGSPCILNTWIPDGAKNHTADKMGYRAILKESLDEIFATEYPRE
                     HMRDALETKLFGIGSEAFVVGSHEFYMNYAARNGVMLCLDMGH"
BASE COUNT          449 a          486 c          584 g          391 t
ORIGIN      
        1 gaaaaaatcg ccaaagcggg tgatccgggc ttcaccgggt tcaccttcgt cgagcgcttt
       61 gcgttctatg agcagtcgaa gaaggcgtat gcgatcgtgg cgaccggcga aagcgccctg
      121 tacgcgaaca tcattctcaa aaagggcgtc gttctctaag ggagccgata ccacatcgag
      181 cggtttttcc gataggagag cggcgtgatt cccatcacgt tgcgaaactg ccggctgaag
      241 tagttggagt cttcgaatcc ggtcgcttcg ctgatctcgc tgaccgacat gtcgcttgag
      301 cggagaagcc ggcacgcatg cttgattctc cgttggatgt ggaattcgat gggggagagg
      361 ccggtcgcgt tcttgaagtg ccggttgagg gtgcttgccg acatgttggt cagttccatc
      421 aattcgctcg ttgaaacgga gcggtcggga tgggcttcca aaaagctgag ggcgtcggca
      481 atccgcatga cggtctggtt gtccttccgc ggctgctcgg cataaaagcg aaacagcatc
      541 accagaagct gcgcaaggat cgcgaacgct ccggccttcg ccccgatctc gctttcggtc
      601 gaatccgatt ccacgagcat gtgttcaaga aggggaagaa cccgcgcctg ctgggcgggg
      661 gtgagctgca tcagcgtcga aagcgagccg tcatggcggt aggagggctc caccttcaaa
      721 agcgcctggt agccgctcat gttgctcaaa tcgaaaaatg attcctcgaa catcgagatg
      781 tcgaagagga tgttgaagag gcgcagatcg cggacatccc gatagccgtg tttaaagccg
      841 ggaggaatga cgaagacgtt tccgctggtc agctcgaacg agccggcggt gtcgaagtgg
      901 gtcccgcttc ccgagacgat gacgatcagt tcccagaagt cgtggctgtg aaggggatag
      961 ggaatttccg gatcccgttc aaggaccttg atggggaagg aagggtcgta aaaatagtcg
     1021 gaagcccgga gtactaagct cattatgaca aaatagtgct aaaatatgcc tattatgtca
     1081 agacaaaaat ggtaaagtgg gggtaacatt atagatagga ggagtgtatg gaaaccactt
     1141 catatcagta cgcgaaagaa cggtaccgaa cgatcggcgt tgataccgat gcagtcctca
     1201 agcggcttga tcggattccg atctcgattc attgctggca gggcgatgat gtcggcgggt
     1261 ttgaagctcc cgatgccgag ctcagcggcg gtggaattca ggtcaccggc tcctatcccg
     1321 gcaaagcgca gacgatcgag cagctccgag ccgatcttga agaggtgctt tcgatcgttc
     1381 ccggtccgca taggatcagc ctccatgcga gctacggcga gttcggctcc gagttcgtcg
     1441 accgaaacga gattgaaccg aaacactttc agggatgggt ggattgggcg caagcgaaca
     1501 acgtccacct tgatttcaac ggaacgtttt tcagccaccc catggcgaat gatggctata
     1561 ccctggcaag caaggatgaa gagattcgtc agttctggat cgagcatgcc aaacgaaccc
     1621 ggaagatcgc agcgtggatc ggaaagcagc agggatcgcc ctgcattctc aatacgtgga
     1681 ttcccgacgg ggcgaaaaac cataccgccg acaagatggg ctatcgggcg atcctcaagg
     1741 agagccttga tgagattttc gccaccgagt atccccgcga gcatatgcgg gacgcccttg
     1801 agaccaagct cttcggcatc ggcagcgaag cgttcgtcgt cggctcgcac gagttctaca
     1861 tgaactatgc cgcccggaac ggggtgatgc tctgcctcga catggggcac
//
LOCUS       DAOPUZ010000261         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_66232_length_1900_cov_3.305691, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000261 DAOPUZ010000000
VERSION     DAOPUZ010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66232_length_1900_cov_3.305691"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..795)
                     /locus_tag="PKN79_04865"
     CDS             complement(181..795)
                     /locus_tag="PKN79_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ95128.1"
                     /translation="MRKIVVLDGYALNPGDLDWTPLEAYGELTVYDRTEPKDVVKRIG
                     DAEIIFTNKTILTGETIIQAPKLKYIGVLATGYNVLDMGAAREKGIVVTNIPAYSTDA
                     VAQFTFALLLEITNRVQRHSDAVIRDNRWTTCKDFCFWDYPLIELANKKMGIIGYGAI
                     GKAVGKVAKALNMEVLRRFNIILDYSRNEAYFKPNNRFSAPFKY"
     gene            complement(825..>1900)
                     /locus_tag="PKN79_04870"
     CDS             complement(825..>1900)
                     /locus_tag="PKN79_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="KamA family protein"
                     /protein_id="HNZ95129.1"
                     /translation="GRFNFELEKLRPKKSWPARLKENMEYFRSDPYLWDILITGGDAF
                     MSSVKSLREILEAVLQMAKDKVEDNEKREEPYAVMKRVRLGTKLPVYLPQRVTGELAD
                     TLAWFKRESAKIGIEQCVIQTHFSSAMEVTPDTEKAVGRILKAGWAVTNQEVFTVAAS
                     RRGHSAKLRKVLNDIGVLPYYNFTVKGFRENRALFATNARSVQELVEESSIGAIAPRY
                     HARIRSFIFNAKEMKEQIDSVRESDEIPFISPDRNTINLPGVGKSNTYRTIGLTDDGR
                     RILRFEFDHTRPHSKVIEEMHHVDIVESKSIARYLRQLEAMGEDPKEYESIWGYSAGE
                     MEERSPVFEGVIESKETPASPLL"
BASE COUNT          334 a          594 c          509 g          463 t
ORIGIN      
        1 ccgcgtgggg cttgttctgg ctcttcgcag gcggcctcgc ctacacgctc ggcgcggtgt
       61 tctttctctt agaacgcatc cgctattttc acttcgcgtg gcacctgttc gtcgtcgcgg
      121 gcactgcgtg tcatgtggtg gcggttgtgg cgtattcgag gtgaattagt cccgtctttg
      181 tcagtacttg aacggcgcgc tgaagcggtt gttcggtttg aaataggcct cgtttcgtga
      241 gtaatcgagg atgatgttga accgtctcag cacttccatg ttgagcgctt tggcgacctt
      301 tcccaccgcc ttgccgatcg ccccgtagcc gataatcccc atcttcttgt tcgcaagttc
      361 aatcagggga taatcccaga agcagaagtc cttgcaggtc gtccacctgt tgtcccgtat
      421 caccgcatcg ctgtgccgtt gcacccggtt ggtgatctca agcaggaggg cgaaggtgaa
      481 ctgggccacc gcgtcagtcg agtaggcggg gatattcgta accacgatgc ctttttctct
      541 cgccgctccc atgtccaaga cattgtatcc cgtcgccaag actccgatgt acttcagctt
      601 cggcgcttgg ataatcgtct ccccggtgag aatcgtcttg ttggtgaaga tgatctcggc
      661 atcgccgatc cgtttgacaa catcctttgg ctccgtccgg tcatatacgg tcaattcacc
      721 gtaggcttca agcggagtcc aatccagatc cccgggattc agggcatatc catccagtac
      781 gacgatcttt ctcatggatg gaatcctacc acgaagatcc gagattatag aagcggtgac
      841 gccggcgttt ccttggattc gatcacccct tcgaagacgg gactccgctc ctccatctcg
      901 ccggcgctgt acccccagat cgactcatac tccttcggat cctcccccat cgcctcaagc
      961 tgccggaggt accgggcgat cgacttggac tcgacgatgt cgacgtggtg catctcctcg
     1021 atcaccttgg agtgcggccg ggtatggtcg aactcgaagc ggaggattct ccgcccgtca
     1081 tcggtaagcc cgatcgtccg gtaggtattg gactttccca ctcccggaag attgatcgta
     1141 ttgcggtcgg gggagatgaa ggggatctca tcgctttctc tgacactgtc gatctgctcc
     1201 ttcatctcct tagcattgaa gataaacgag cggatcctgg cgtggtagcg cggcgcgatc
     1261 gcaccgatgc tcgactcctc aaccagctcc tgcaccgaac gggcgttcgt cgcaaacagc
     1321 gcccggttct ccctgaaccc cttcaccgtg aagttgtagt agggaagcac tccgatgtcg
     1381 ttgagaacct tcctcagctt cgccgagtgt ccccttcggc tcgccgccac ggtgaacacc
     1441 tcctggttcg tcaccgccca gccggccttc agaatccgac ccaccgcctt ttccgtatcg
     1501 ggggtgacct ccatcgccga ggagaagtgg gtctggatca cgcactgctc gatcccgatc
     1561 ttcgcgctct cccgcttgaa ccaggcaagg gtgtcggcaa gctccccggt gacccgctgg
     1621 ggaagataga cgggtagctt cgttccgagg cggacccgct tcatcacggc atagggctct
     1681 tccctcttct cgttgtcttc aaccttgtcc ttcgccatct ggagcaccgc ttccaggatc
     1741 tccctcagcg acttcaccga gctcatgaac gcatcccctc cggtaatcag gatgtcccag
     1801 aggtacggat cgctgcggaa gtactccatg ttttccttta gccgggccgg ccagctcttc
     1861 ttcggtcgga gcttctcaag ctcgaagttg aagcgcccca
//
LOCUS       DAOPUZ010000262         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_66244_length_1900_cov_2.812466, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000262 DAOPUZ010000000
VERSION     DAOPUZ010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66244_length_1900_cov_2.812466"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..433
                     /gene="kduI"
                     /locus_tag="PKN79_04875"
                     /pseudo
     CDS             1..433
                     /gene="kduI"
                     /locus_tag="PKN79_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606964.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            439..1242
                     /locus_tag="PKN79_04880"
     CDS             439..1242
                     /locus_tag="PKN79_04880"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HNZ95130.1"
                     /translation="MDFLKDQFSVKGKVAWVTGGSYGIGLSIALAYAKAGAKIAFNDI
                     NQELVDKGLAAYKEAGFDVKGYVCDVTDEQAVQKTAKLIEQDLGPVDILVNNAGIIRR
                     TPMHEMSIEDWRLVVDIDLTAPFIVAKAVLPSMMERRSGKIINVCSMMSELGRETVAA
                     YAAAKGGLKMLTRNIASEYGKYNIQCNGIGPGYIATPQTAPLREKQADGSRHPFDSFI
                     IAKTPAERWGTPEDLMGPALFLGSKASDFVNGHVLYVDGGILAYIGKQP"
     gene            1430..>1900
                     /locus_tag="PKN79_04885"
     CDS             1430..>1900
                     /locus_tag="PKN79_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNZ95131.1"
                     /translation="MTNKKKKYVYFFGSGKVEGTVKMRDLLGGKGANLADMTSIGLPV
                     PPGFTISTEACAYYSANDGAYPEGLREEILEHLATLEKMMGAKLGDAANPLLVSVRSG
                     AASSMPGMMDTILNLGLNPKSVQGLIAKTGNERFAWDSYRRFMQMFGDVVMGVPH"
BASE COUNT          472 a          491 c          544 g          393 t
ORIGIN      
        1 ggcgttcccc gccaagcaca tcgtcttcgc cgaggcgaag cacgttcccg ccggagccgc
       61 cgccgactcc aacaagcgga cgatccacca atacatccac cccgaggttc tcgacacctg
      121 tcagctttcg atggggttga cggagctgga aagcggcagt gtgtggaaca cgatgcccgc
      181 ccactcccac gagcgccgga tggaggtgta cttctatttc gaggttaaga aggatcagac
      241 cgtcttccac ctgatgggcg aaccgacaga aacccgccac atcatcgttc acaatgagga
      301 agcggtcatc aacccctcgt agtcaattca cagcggagtg gggacgagca actacacgtt
      361 catctggtcg atggccggtg aaaaccggac ctataccgat atagactgga tcgccacgga
      421 agatttgagg taaagaagat ggattttttg aaagatcaat tctccgtcaa ggggaaggtc
      481 gcctgggtga ccggcggcag ttacggcatc ggtttgagca tcgcattggc atatgccaag
      541 gcgggagcga agatcgcgtt caacgacatc aatcaggagc tggtggacaa gggacttgcc
      601 gcctacaaag aagcggggtt cgatgtaaaa ggatatgtgt gcgatgtgac ggatgaacag
      661 gcggtccaaa aaacagcgaa gctgatcgaa caggatctcg gaccggtcga tatcctcgtg
      721 aacaacgccg gcatcattcg gcgcaccccg atgcacgaga tgagtatcga ggattggcgg
      781 ctggtcgtcg atatcgattt gaccgccccc ttcatcgtcg ccaaagcggt gcttccctcg
      841 atgatggagc gaagaagcgg aaagatcatc aacgtctgtt cgatgatgag cgagctggga
      901 agagaaaccg tcgcagccta tgcggcggcg aagggtggat tgaagatgct gacccgcaac
      961 atcgccagtg aatatgggaa atacaatatc cagtgcaacg gaatcgggcc cgggtacatc
     1021 gccacgccgc agactgcgcc gctgagggag aagcaggcgg acggatcgcg ccatcccttc
     1081 gattccttca tcatcgccaa gacacccgcc gagcgctggg gtactcccga ggatctgatg
     1141 ggtccggctc tcttcctcgg ttcgaaggcg agcgacttcg tgaacggcca tgtgctctat
     1201 gtcgatggag gaattctcgc ctacatcggc aaacaaccgt aaggtgatac agaatcaatc
     1261 acggggaagg atgcaccttc cccgtttccg tttttggtac aatgttttac tttttactcc
     1321 gctcaaatag tttctgaaca agaaattgac tctctgttga ttgacagtaa gcggaggaac
     1381 cgataggatt tgttcgttat caatctgaaa taatttagag gagtcatgaa tgacgaacaa
     1441 gaaaaaaaag tatgtctatt tctttggttc aggaaaggtt gaaggaacgg tcaagatgcg
     1501 ggatctgctc ggtggcaaag gcgcgaacct cgccgatatg accagcatcg gtcttccggt
     1561 gcctccgggc ttcacgatca gcaccgaagc gtgcgcgtac tactccgcca atgacggagc
     1621 gtatcccgaa ggccttcggg aagaaatcct tgaacatttg gctacccttg aaaagatgat
     1681 gggtgcaaaa ctcggggatg ccgccaaccc gctgctggtc tcggtccgaa gcggagccgc
     1741 ctcttcgatg ccggggatga tggatacgat tttgaacctc ggactcaatc cgaaaagcgt
     1801 ccaaggtctt attgcaaaaa ccggtaatga gcgattcgct tgggatagct atcgccgctt
     1861 catgcagatg ttcggcgatg tcgtcatggg ggttccccac
//
LOCUS       DAOPUZ010000263         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_66314_length_1899_cov_2.848698, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000263 DAOPUZ010000000
VERSION     DAOPUZ010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66314_length_1899_cov_2.848698"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PKN79_04890"
     CDS             <1..709
                     /locus_tag="PKN79_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNZ95132.1"
                     /translation="RISWRMYVQGWGVPFEDEEAKRIIAEAKELLAREEIASSFERGM
                     KVVYSMMKVKSDRHTISTGRHVFHFLRDEGSGFSLPDMVAQEDAVGFFATSSALDLEA
                     ILDDDPVKTLTLKLLADRLAEVLAQEAQSLIADLWVERPRSIIRPAIGYPSWSDHSEK
                     KTLFELLEVKERIGISLSSSYAMNPPSSVCGAVIGGEGVRYFTVKDVSRAQLERYARR
                     KGVPDRELASFLKDMGY"
     gene            713..1666
                     /gene="metF"
                     /locus_tag="PKN79_04895"
     CDS             713..1666
                     /gene="metF"
                     /locus_tag="PKN79_04895"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNZ95133.1"
                     /translation="MRVTEIIQASKKPLFTFELVPPLKGRTLEPLMETIRTLMEFDPS
                     YINVTNHQAEVVYIEREDGLLQRKTVRKRPGTTALAALIQHTFDVPTVAHLICGGMSR
                     EQIEDVLIELHFLGIENILALRGDPPAGEKRFIPMPDGHAYSSQLVGQIVDLNHGRYL
                     DDQYRDGEATRFSIGVAGYPEKHAEALNMTVDLANLKKKVDAGAQYIVTQMFFDNGKY
                     FSFVNACRSIGITVPIIPGLKPIASKRDLSLIPQTFGVDLPEALTSELEGVDSNAEIT
                     EIGVQWCIAQSKELYAAGVPSIHYYTLGKARNVARIVRAVY"
     gene            complement(1663..>1899)
                     /locus_tag="PKN79_04900"
     CDS             complement(1663..>1899)
                     /locus_tag="PKN79_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNZ95134.1"
                     /translation="ICVDCHVHRIANRLGWIATGTPEESEQALMKVMPRRFWIPLNEL
                     LVRYGQVICISVSLFCSRCPEETVCPRVGVTKTR"
BASE COUNT          403 a          558 c          564 g          374 t
ORIGIN      
        1 gcggatcagc tggcggatgt acgttcaggg gtggggcgtt ccctttgagg atgaggaggc
       61 gaagcggatc atcgccgagg cgaaggagct tcttgccagg gaagagatcg catcctcctt
      121 cgaaagggga atgaaggtcg tgtattcgat gatgaaggtc aaaagcgacc gccatacgat
      181 ttccaccggt aggcatgtct ttcatttcct tcgggatgaa ggaagcggct tcagccttcc
      241 cgatatggtg gcccaagagg acgccgtcgg cttcttcgcc acctcctccg ctcttgatct
      301 ggaggcgatc ctcgatgacg atccggtaaa gaccctgacc ctgaagctgc ttgccgaccg
      361 gcttgccgag gttcttgctc aggaggctca atccctcatc gccgatctgt gggtggaaag
      421 accccgctcg atcatccggc ccgcgatcgg ctacccctca tggagcgacc acagcgagaa
      481 gaagaccctg ttcgagctgt tggaggtgaa ggagcggatc ggcatctcgt taagctcgag
      541 ctatgcgatg aatcctccat cgtcggtctg cggagcggtc atcgggggcg aaggggtccg
      601 atatttcacc gtcaaggacg tgagccgggc gcagcttgag cgctacgccc gaagaaaggg
      661 cgttcccgat cgggagcttg cctcctttct gaaggatatg ggatactgag ccatgcgtgt
      721 aaccgaaatc atccaagcct ccaaaaaacc cctcttcacc ttcgaactgg ttccgccgct
      781 caaaggccgg acgctcgagc ccctgatgga aacgatccgg accctgatgg agttcgaccc
      841 ttcctacatc aacgtgacga accaccaggc ggaggttgtc tacattgagc gggaggatgg
      901 gctccttcag cggaagacgg tacgcaagcg ccccggcacc accgccctgg cggcgctcat
      961 ccagcacacc ttcgacgttc cgacggtggc gcacctgatc tgcggcggga tgagcaggga
     1021 gcagatcgaa gacgtcctca tcgagcttca ctttcttgga atagagaaca ttctcgccct
     1081 cagaggcgac cctcccgccg gggagaagcg cttcataccg atgcccgacg ggcatgccta
     1141 ctcaagccag ctggtcgggc agatcgtaga tctcaatcac ggaaggtatc tggatgatca
     1201 ataccgggac ggggaagcga cccggttctc gatcggagtg gccggatacc ccgagaagca
     1261 tgccgaagcg ctgaacatga cggtcgacct ggcgaacctg aagaagaagg tcgatgccgg
     1321 ggcacagtac atcgtcaccc agatgttttt cgacaacgga aagtattttt ccttcgtgaa
     1381 cgcgtgccgc tcgatcggga tcaccgtccc catcatcccc ggtctcaagc ccatcgcctc
     1441 gaagcgggat ctcagcctta ttccgcaaac cttcggcgtc gatcttcccg aagcgttgac
     1501 ttccgagctt gaaggggtcg attccaacgc cgagatcacc gaaatcggcg ttcaatggtg
     1561 catcgcccag tcgaaagagc tctatgccgc aggggttccc tccattcact attatacgct
     1621 ggggaaagcc cggaacgtgg cccggatcgt tcgcgccgtc tattaacggg ttttggtcac
     1681 tccgactctg ggacacaccg tttcttccgg acagcgggag cagaagagac tgaccgaaat
     1741 gcagatcacc tgtccgtagc ggacgagcag ttcgttcaag gggatccaga agcgccgggg
     1801 catcaccttc atcagcgcct gttcactctc ttcaggagtc ccggtggcga tccagccgag
     1861 acggttggcg atccggtgga cgtggcagtc gacgcagat
//
LOCUS       DAOPUZ010000264         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_66442_length_1896_cov_4.124389, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000264 DAOPUZ010000000
VERSION     DAOPUZ010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66442_length_1896_cov_4.124389"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PKN79_04905"
     CDS             complement(<1..742)
                     /locus_tag="PKN79_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ95135.1"
                     /translation="MLKRFVSYYRPYKGLFLLDLGMAVLSSILAILFPALTRHLLSVE
                     IPAGNLRAMVVIFVLMIAIYLIQTVSQYIRITWGHILGVKMETDMRNELFAHLQRLSF
                     GYFDRTKTGHMMSRITNDLFIIAEVAHHGPEDFLISLATIVGSYVLMFIYSPPLALIS
                     LIPFPFMVFYGSYFGHKMRVTFRRVRSTVADVNSNVENSLMGIREVKSFGRETFQTEK
                     FQEVNNILKESKMQQYKVMGSYHALMGLF"
     gene            complement(742..>1896)
                     /locus_tag="PKN79_04910"
     CDS             complement(742..>1896)
                     /locus_tag="PKN79_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNZ95136.1"
                     /translation="DVYVATPGRILDYQKMKKVDFRQFDIFVVDEADRLFDMGFYPDI
                     QKMFSLLRPVKERQTMLFSATLSTKVRHLAWQFMNEPKEIEVQPEEITVKEITQELYH
                     ISKDEKFGLLLQLLKQDEPASCLIFTNTKARCVEVSKRLSMNGYQSKYLMGDLPQRKR
                     LEIIGWMKSGKLRFLVATDVAARGLQIDDLALVVNYDIPEDYESYVHRIGRTARAGKS
                     GKSITLADEEYVFNLEAIEEFIQMKIPVIWPEDLPEVEDKSASYTFRDLVPRTGVRSS
                     SARRGGTPSPSRGRKQAPRRAPERKQRRPRPAAPPKQHPPRRSAKAYADLSALPLEER
                     LAYYKKEYLGNQQPADEPVKKTSSPKPVDAKEEQAKKKGFFARLFANRGK"
BASE COUNT          412 a          527 c          518 g          439 t
ORIGIN      
        1 tgaagagccc catcagggcg tggtaggaac ccatcacctt gtactgctgc atctttgatt
       61 ctttgagaat gttgttcacc tcttggaact tctccgtctg aaaggtttcc cggccgaacg
      121 atttgacttc ccgaatcccc atcagcgagt tctccacgtt gctgttcaca tcggcgacgg
      181 tcgagcggac ccgacgaaac gtcacccgca tcttgtgtcc gaaatacgac ccgtaaaaca
      241 ccatgaacgg gaagggaatc agactgatca acgcaagcgg tggactgtag atgaacatca
      301 ggacatagga gccgacgatc gtcgccaagc tgatcagaaa atcctcaggg ccatggtgcg
      361 ccacctcggc gatgatgaac aaatcgttgg tgatccggct catcatgtgt ccggtcttgg
      421 tccgatcgaa atacccgaac gagagccgct gaaggtgggc aaacagctca ttgcgcatat
      481 ccgtttccat cttcaccccg aggatatgcc cccaagtgat ccggatatat tgactgacgg
      541 tctggatcag atagattgcg atcatgagga caaagatcac caccatcgcc cgaagatttc
      601 cagcgggtat ttcaacgctc aaaaggtgcc gggtcagcgc agggaacaga atggcaagga
      661 tggaagacag caccgccatc cccagatcca acaagaacaa acccttgtag gggcgatagt
      721 acgagacaaa acgcttcagc atcatttccc ccggttcgca aacaatcggg caaagaatcc
      781 tttcttcttc gcttgttcct ctttggcatc aaccggtttt ggtgaagaag ttttctttac
      841 cggctcatcc gccggttgtt gattcccaag atattccttt ttgtagtaag caagccgctc
      901 ttccagcgga agggcggaaa ggtcggcgta tgctttggcg ctgcggcgcg gcggatgctg
      961 cttcggcggt gcggccggcc tcgggcggcg ttgtttccgt tccggggcgc gcctgggcgc
     1021 ctgtttcctg ccccggctcg gtgaaggggt gcccccacgg cgcgccgatg aggatcggac
     1081 tccggtccgc ggaaccagat cgcggaacgt gtaggatgcg ctcttgtcct cgacctcggg
     1141 aagatcctcc ggccagatga ccggaatctt catctggatg aactcctcga tcgcctcaag
     1201 gttgaagacg tactcttcat cggcgagagt gatcgatttg ccgctctttc cggctcgggc
     1261 ggtccggccg atccggtgga catagctttc gtaatcttcg gggatgtcgt agttgaccac
     1321 cagagcgagg tcgtcgatct gaagaccccg cgcagcgaca tccgttgcga cgaggaagcg
     1381 gagctttccg ctcttcatcc acccgatgat ctcaaggcgc tttcgctgcg gcagatcgcc
     1441 catcagatat ttgctctgat atccgttcat gctcagtcgc ttggagactt caacacagcg
     1501 agctttcgtg ttggtgaaga tcaggcagga ggcgggctca tcctgcttca gcagctggag
     1561 caagagaccg aacttttcat ctttcgaaat gtggtacagc tcctgggtga tctccttgac
     1621 ggtgatctcc tcgggttgaa cctcgatctc tttcggctcg ttcatgaact gccatgcaag
     1681 gtgacgaacc tttgtggaca gcgtcgcact gaaaagcatc gtctggcgtt ctttcaccgg
     1741 ccggagcaag ctgaacattt tttggatgtc cggatagaag cccatatcga agaggcgatc
     1801 cgcttcatcc acgacgaaga tgtcgaattg acgaaaatcg acctttttca tcttttgata
     1861 atccagaatg cggccgggcg tcgctacata cacgtc
//
LOCUS       DAOPUZ010000265         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_66696_length_1891_cov_3.389978, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000265 DAOPUZ010000000
VERSION     DAOPUZ010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66696_length_1891_cov_3.389978"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="PKN79_04915"
     CDS             <1..176
                     /locus_tag="PKN79_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ95137.1"
                     /translation="APFASWGSLVGDAVDGMTLYPWRLIFPAIAMTLFLFAMNFFGDG
                     LRDAFDPQSKNEL"
     gene            189..1223
                     /locus_tag="PKN79_04920"
     CDS             189..1223
                     /locus_tag="PKN79_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ95138.1"
                     /translation="MSPVNDREVVLDVQNLKTYFKTDAGIVKAVDGVSFTLHKGQTLG
                     IVGESGCGKSVTNLSIMRLIPSPPGKVVGGEAIFEGTDILALDEKEMRTIRGNKISMI
                     FQDPMTSLNPFLRISTQMIETIRLHTKGISKKEALERSIEMLKLVGIPSAEQRVHAYP
                     HQFSGGMRQRVMIAMALSCNAEILIADEPTTALDVTIQAQILELIQNLSKKMGTSVII
                     ITHDLGVVAGMCDEVCVMYAGRIVEKAETEKLYAFPAHPYTEGLIRSVPRMDTTKKGQ
                     RLYSIEGQPPNVIDLPPCCPFHPRCPKAMDVCRASYAPITDLGGGHEVACWLYNSEEE
                     KAAALKEVQA"
     gene            1220..>1891
                     /locus_tag="PKN79_04925"
     CDS             1220..>1891
                     /locus_tag="PKN79_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ95139.1"
                     /translation="MKQPILQVRDLKQHFPITSGMILQRQVGAIRAVDGISFDVFEGE
                     TLGIVGESGCGKSTAVRSISQLYKPTGGSVIFNGIELTTADPKTMLTARRDIQMIFQD
                     PYASLNPRMTVRSIIAEPLVIYNRRKLLDPPLSKLEIEHRVEELMERVGLSKVFKNRY
                     PHEFSGGQRQRIGIARALALKPKIILADEPVSALDVSIQSQILNLLSDLQKEFNLTYI
                     FIAHDL"
BASE COUNT          435 a          542 c          527 g          387 t
ORIGIN      
        1 aagccccgtt cgcttcgtgg ggttcgctcg tcggcgacgc ggtcgacggg atgaccctct
       61 atccgtggcg cctgattttc cccgcgatcg ctatgaccct ctttttgttc gctatgaact
      121 tcttcggaga cggcctgcgc gacgcgttcg acccgcaatc aaagaatgag ctgtaggagt
      181 atgatggcat gagtccagtc aacgatagag aggtggtctt agacgttcaa aacctcaaaa
      241 catatttcaa gaccgacgcc ggcatcgtca aggcggtcga cggggtctcc ttcaccctgc
      301 acaaaggaca aactctggga atcgtcggtg agagcggctg cggaaagagt gtaacgaatc
      361 tttcgatcat gcgtctgatt ccctctcctc cgggaaaggt ggtcggcgga gaggcgatct
      421 tcgaggggac ggacatcctt gctctggatg agaaagagat gcgcacgatc cggggcaaca
      481 agatttccat gatcttccag gacccgatga caagcctgaa tccctttttg agaatttcaa
      541 cgcagatgat cgaaacgatc cgcctccata cgaagggcat cagcaaaaaa gaagcgcttg
      601 agcgctccat cgaaatgctc aagctcgtgg gaataccgtc ggcggagcag cgggttcacg
      661 cttatcctca tcagttctcc ggcggtatgc gccagcgggt gatgatcgcg atggcgcttt
      721 cctgcaatgc ggagatcctc atcgccgacg agccgaccac ggcgttggat gttaccatac
      781 aggctcagat tctcgaactc atccagaatc tgagcaaaaa aatgggcacc agcgtcatca
      841 tcatcaccca cgacctgggc gtcgtggccg gaatgtgtga tgaagtgtgc gtgatgtacg
      901 ccgggagaat cgtggagaaa gcggaaacgg aaaagctcta cgcgttcccc gcccacccct
      961 acaccgaagg cctgatcaga agcgttcccc ggatggacac tacgaagaaa ggccagcggc
     1021 tctattcgat cgaaggccag ccgccaaacg tgatcgacct tcccccttgc tgtcccttcc
     1081 atccccgttg tccgaaggcg atggatgtgt gccgcgcatc atatgcgccg atcaccgacc
     1141 tcggcggggg gcacgaggtc gcctgctggc tctataacag tgaagaagag aaggctgcag
     1201 ccttgaagga ggtgcaagca tgaaacagcc tattctccaa gtccgagatc tgaagcagca
     1261 ttttccgatt acttcgggaa tgatcctcca acgccaggtc ggagcgatcc gggcggtcga
     1321 cggcatctcc ttcgatgtgt ttgaagggga aactctggga atcgtcggag agtcggggtg
     1381 cggaaaatcc actgcggtcc gctcgatcag tcagctctat aagccgaccg gcggctcggt
     1441 gatcttcaac ggaatcgaat tgaccaccgc cgatcccaaa acgatgctca ccgcccggcg
     1501 ggatatccag atgatctttc aggatcccta tgcatcgttg aacccccgga tgacggtgcg
     1561 ttcgatcatc gccgagcctt tggtaatcta taaccgccgg aagctgcttg atccgccgct
     1621 ttccaagctt gagatcgaac accgggtcga agaactgatg gaacgggtcg gtctttcaaa
     1681 ggtgttcaag aaccgctacc cccacgagtt cagcggcggg cagcgccaga gaatcggaat
     1741 cgctcgagcc cttgcgctca agccgaagat cattctggcg gatgagccgg tctccgctct
     1801 ggacgtctcg atccagtcgc aaatcctcaa tctgctcagc gacctgcaga aagagttcaa
     1861 cctgacctat atcttcatcg cccacgacct c
//
LOCUS       DAOPUZ010000266         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_66874_length_1888_cov_2.442444, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000266 DAOPUZ010000000
VERSION     DAOPUZ010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66874_length_1888_cov_2.442444"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKN79_04930"
     CDS             <1..344
                     /locus_tag="PKN79_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNZ95140.1"
                     /translation="GDIIVADANFGCGSSREHAPIAIKESGVSCVIARSFARIFYRNS
                     INIGLPILECPEACADIKAGDTVVVDFSTGVITNKRSGNTFQSEPFPPFMQELIQEGG
                     LANYVAKGGIA"
     gene            344..1396
                     /gene="leuB"
                     /locus_tag="PKN79_04935"
     CDS             344..1396
                     /gene="leuB"
                     /locus_tag="PKN79_04935"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNZ95141.1"
                     /translation="MEKKRIAVIAGDGIGVEITREALNVLKKIAPDGFVFEHYKAGGE
                     ALDAFGVPLPEETLNGCKQSDAVLLGSLGGPKWDTVPSHLRPEKGLLGLRGGLGLFCN
                     LRPAILYPQLAGACPLKNELVEGGLDIMVVRELTGGIYFGERGREGDSAFDTERYSKA
                     EVERIGRKAFEIAMNRSKRLCSIDKANILISSQLWRETINALSKEYPEVSVTHMYVDN
                     AAMQLVRNPRQFDTIVTNNIFGDILSDEAAQITGSIGMLPSASLGEGGVGMYEPIHGS
                     APDIAGKDIANPIAMILSAALMLRHSFGMEREAKRIEDAVSGVLDQGYRTADIMSGGM
                     TLVGTNEMGRRITEAL"
     gene            1409..>1888
                     /locus_tag="PKN79_04940"
     CDS             1409..>1888
                     /locus_tag="PKN79_04940"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNZ95142.1"
                     /translation="MKRRSAQMTEGPQRAPHRSLMKALGWTDSEIHMPIIGVVSGANE
                     IIPGHIHLDRIVEAVKSGVRHGGGNPVAFPVIGVCDGIAMGHTGMKYSLVSRELIADS
                     IEVVANAHPFDALVFVPNCDKIVPGMLMAAARINIPSIFVSGGPMLAGKKDGREPINL
                     "
BASE COUNT          403 a          535 c          601 g          349 t
ORIGIN      
        1 agggggatat catcgtcgcc gacgcgaact tcggctgcgg ctccagccgg gagcacgctc
       61 cgatcgcaat caaggagagc ggagtctcgt gcgtcatcgc ccgctccttc gcccggatct
      121 tctaccgcaa ctcgatcaac atcggcctgc cgatcctgga atgccccgag gcgtgcgccg
      181 acatcaaggc cggagacaca gtcgttgtgg atttctccac cggggtgatc accaacaagc
      241 gaagcgggaa caccttccaa agcgagccgt ttcccccctt catgcaggag ctgattcagg
      301 aaggcggcct tgcgaactat gtggccaagg gagggatcgc gtgatggaaa agaaacggat
      361 cgcagtcatc gccggtgacg gcatcggggt ggagatcacc agagaagcgc tcaacgtgct
      421 gaagaaaatc gccccggacg gttttgtctt tgaacactac aaagcgggag gcgaagccct
      481 tgatgccttt ggagttcctc ttcccgagga gacgctgaat ggatgcaaac aaagcgatgc
      541 tgtcctgctc ggatcgctcg gggggccgaa atgggacacg gtcccttcgc atctgcgccc
      601 ggaaaagggg ctcttgggtc tgcgcggcgg gctcgggctg ttctgcaacc tccgtccagc
      661 aatcctgtat ccccagcttg ccggggcgtg tccgttgaag aacgagctcg tcgaaggcgg
      721 gttggacatc atggtggttc gggagctcac cggggggatc tacttcggag agcggggccg
      781 ggaaggggat tcggcgttcg atacggagcg ctacagcaag gcggaggtcg agcggatcgg
      841 acgaaaagcg ttcgagatcg ccatgaaccg aagcaaacgg ttgtgttcga tcgacaaggc
      901 gaacatcctg atttccagcc agctgtggcg cgagacgatc aatgccctct ccaaggagta
      961 tcccgaggtt tcagtgactc atatgtacgt cgataatgcg gcgatgcagc tggtgaggaa
     1021 tccccgtcag ttcgacacga tcgtcaccaa caacatcttc ggggatatcc tcagcgacga
     1081 ggcggcccag atcaccggct cgatcgggat gctgccttcc gcctccctcg gcgagggagg
     1141 ggtggggatg tatgagccga tccacggttc cgctccggac atcgccggaa aggatatcgc
     1201 caacccgatc gcaatgattc tttccgcagc gttgatgctt cgccactcct tcgggatgga
     1261 gcgtgaagcg aaacggatcg aggatgcggt aagcggcgtg cttgatcagg ggtatcgaac
     1321 cgctgacatc atgagcgggg ggatgaccct cgtcggaacg aacgagatgg ggcgccgcat
     1381 aaccgaggcg ctttgaggag gatgtgatat gaaacgtcgt tcagcacaga tgaccgaagg
     1441 gccgcagcgc gccccccaca gatcgttgat gaaagcgctg ggctggaccg attccgagat
     1501 ccatatgccg atcatcggag tcgtcagcgg agccaatgag atcattcccg ggcacatcca
     1561 cctggatcgg atcgtcgagg cggtgaagag cggagtccgc cacggcggag gaaaccccgt
     1621 cgcctttccg gtgatcgggg tctgcgacgg aatcgccatg ggacatacgg ggatgaagta
     1681 ctccctcgta agccgggagc tgatcgccga ctcgatcgaa gtagtggcca acgcccatcc
     1741 cttcgacgca ttggttttcg ttccaaactg cgacaagatc gtccccggaa tgctgatggc
     1801 cgctgcccga atcaacatcc cctcgatctt cgtttccggc ggtccgatgc ttgccgggaa
     1861 aaaggatgga cgtgagccga tcaacctc
//
LOCUS       DAOPUZ010000267         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_66983_length_1886_cov_2.928454, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000267 DAOPUZ010000000
VERSION     DAOPUZ010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66983_length_1886_cov_2.928454"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PKN79_04945"
     CDS             complement(<1..917)
                     /locus_tag="PKN79_04945"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNZ95143.1"
                     /translation="MKLYRRYIFKSTINVGLGALFLTTLMLISVDLFANLDTYLSHEL
                     PLATIAYLTLLGAPQAVLFALGPSLLFSATFFLSQLHANNEYICLIGSGIRPLKIILP
                     IIAVGFIASILQFAFAEYVKLPLSKERLILEEELFGLRSTQDNRNITLHDPEGRYVLY
                     ARHYRAQDNLLREVLLLLFDEQGNLTHRLDAQQGRYDEDSGQWRFHGVQSQEVGEVVS
                     TRTLEAYDDPRINLDPSYFRGAGGDIDQMDIQTALQYLHWMKRLDPNRYTPLAADFVK
                     RILDALNPLVLIVIAAAIRYSLKKNILLFA"
     gene            complement(914..>1886)
                     /locus_tag="PKN79_04950"
     CDS             complement(914..>1886)
                     /locus_tag="PKN79_04950"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNZ95144.1"
                     /translation="LGDLGEHREILALRCNGISPIRLYLPILLISLGITAATFITADY
                     ILPVSSARYRELYTSLMQNLPTLELVDGGINTIGNKTLINKHVNENRIDELILFDSSS
                     REDGKILTAPSATVTLDDPLNFVYLLELDRPRLLYTRSGGEWALAQAERARLFLDFSK
                     QVGSLGTVLPSQLSSKQLQAEIAQYRISFDEEMKHYNERLSALREEVKAGTKSKAELK
                     EFKKQKPINFYLQYYTAELHKKYALSTACTILLFLTFVLAHLRLRHGKLIGFGLSMIT
                     AVIYWYLLFFAQLQIFTYPISAALFIWAPNIILGTLGILLLLIMRRS"
BASE COUNT          475 a          444 c          546 g          421 t
ORIGIN      
        1 gcaaagagaa ggatgttctt tttcagcgag tatcggatcg cggcggctat gacaatgaga
       61 accagcggat tcaatgcatc caggatccgt ttaacgaaat ccgcagccag cggtgtatag
      121 cgattcggat caaggcgctt catccaatgc agatactgaa gagccgtttg gatatccatt
      181 tgatcgatgt caccgcccgc tcccctgaaa taggaaggat cgagattgat ccgagggtca
      241 tcataggctt caagagttct cgttgaaacc acctccccca cctcctgact ttgcacgccg
      301 tgaaacctcc attgcccgct atcttcatca taccggcctt gttgggcatc gaggcggtgg
      361 gtgaggttcc cctgctcatc gaagagaagg agcagcacct cccgcaatag gttgtcctga
      421 gctcgatagt gacgggcgta gaggacatac cgtccctcgg gatcgtggag ggtgatgttc
      481 ctgttgtcct gcgtcgatcg cagtccaaac agctcctctt cgagaataag ccgctctttg
      541 gaaagcggaa gcttcacata ttcggcaaaa gcaaactgga ggatcgaagc gatgaatccg
      601 accgctatga tcgggaggat gatcttcaaa ggccggattc cgctgccgat caggcagata
      661 tactcattgt tggcatgaag ctgggagaga aagaaggtcg cactgaacag cagggaggga
      721 ccgagggcga agagcaccgc ctggggggcg ccgagaagcg taagatatgc gatcgtggcg
      781 agcggaagct catgggagag ataggtgtcg aggttggcga acagatcgac gctgatgagc
      841 atcagggtcg tcaggaagag tgcgcccaaa ccaacgttga tggtactttt gaagatatag
      901 cggcggtaga gtttcatgag cgcctcatga tgagaagcag aagaatcccc aacgtgccga
      961 ggatgatgtt cggagcccag atgaacagcg cggcggagat cggataggtg aaaatctgca
     1021 gctgggcgaa aaagagcaga taccagtaga tgaccgcggt aatcatggat agtccgaagc
     1081 cgatcagttt gccgtggcgg agacgaagat gggccaggac gaaagtgaga aagagcagga
     1141 tcgtgcacgc cgtgctcaac gcatattttt tgtgcaattc cgccgtgtag tattgaaggt
     1201 aaaaattgat cggtttttgt tttttgaact ccttgagttc cgctttgctt ttggttcccg
     1261 ccttgacctc ctcccgcaga gcgctcaatc gttcattata gtgcttcatc tcttcatcga
     1321 agcttattcg gtattgagca atctcggctt gaagctgttt actgcttagt tgactcggta
     1381 gaacggtgcc caatgaaccg acctgtttgg agaaatccaa aaaaagccgg gctcgctcgg
     1441 cttgggcaag cgcccactcg cccccgcttc gggtgtaaag aagccggggt cggtccagtt
     1501 caagaagata gacgaaattc agcggatcat ccaacgtcac ggtcgccgag ggtgctgtaa
     1561 ggatttttcc atcctctcgg gatgaagagt cgaagagaat caattcatcg atcctgtttt
     1621 cgttgacatg tttattgatg agggtcttat tgccgatcgt gttgattcct ccatccacca
     1681 attcaagagt gggcagattc tgcatcagcg aggtatagag ctcccgatac cgggctgaag
     1741 aaacaggcag gatataatcg gcggtaataa acgtggcggc ggtaatgccc aatgaaatca
     1801 ggagaatggg cagatagaga cggatcggag agattccgtt gcatcgcaag gcaagaatct
     1861 cccggtgctc gccaaggtct cccaag
//
LOCUS       DAOPUZ010000268         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_67574_length_1875_cov_3.578571, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000268 DAOPUZ010000000
VERSION     DAOPUZ010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67574_length_1875_cov_3.578571"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PKN79_04955"
     CDS             <1..1112
                     /locus_tag="PKN79_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNZ95145.1"
                     /translation="AIMPAYTLAKILHLKNHRTEAYDRLAHLLLPHDWVNFYLTGEYF
                     TEAGDASGTAYYSPEKRAWEPEMLRAVDEKKHLEHMLPTLIESYEIGGRLCKTAAEEL
                     GLRPGIVVATGAGDNMASAIGTGCTSGGDLVMSMGTSGTLFGYSDTLVADREGRLSAF
                     CSSTGGYLPLLCTNNCTLSTEVIRNLFSRDVKTLDMLAGKAPIGSQGVTVLPFFTGER
                     VPYLPYGKGVIAHLDSQNTTEENIARAALESAVFALRGGMDTFSELGFTPSRLILTGG
                     GANSKIWRQIASDVFNLAVIVPSSTESAALGVALQALWALEGGSITEIANEHVLFDED
                     KMHQPDRMAVEQYAEAYRRYSTYVQALTPIFIRRI"
     gene            1112..>1875
                     /locus_tag="PKN79_04960"
     CDS             1112..>1875
                     /locus_tag="PKN79_04960"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606587.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNZ95146.1"
                     /translation="MSYFVGEKEYFKGIGKIRFEGKASRNPLAFKHYNAGERVGDKTM
                     AEHLRFSTAYWHSFCADGSDPFGKPTIVFPFDSSDPFAKADAAFEFFTKLGTPFYAFH
                     DVDVFNDIDDGRAYEKRYQEVADGLLARQKASGVKLLWNTSNVFSHPRYMNGAATNPD
                     FNVVARAAVQIRKSLEVNVQLGGLNYVFWGGREGYMSLLNTNMKREQEHLARMLIIAR
                     DYARSIGFTGKFLIEPKPMEPTKHQYDYDAATTIGFL"
BASE COUNT          426 a          528 c          527 g          394 t
ORIGIN      
        1 atgcgatcat gcccgcctat acgctggcga agattcttca cttgaagaac catcgaacgg
       61 aagcgtatga ccgattggct cacttgcttc ttccccatga ctgggtcaac ttctatctca
      121 ccggcgagta tttcaccgaa gccggagatg cttcagggac agcctactac agtcccgaga
      181 aacgggcttg ggagcctgag atgctccggg cggtcgatga gaagaaacac ctcgaacata
      241 tgcttccgac cctcatcgaa tcgtatgaga tcggaggacg gctctgtaaa accgccgccg
      301 aagagcttgg actgagaccg gggattgtcg ttgccaccgg agccggggac aacatggcca
      361 gcgcgatcgg aacgggatgc acctccggag gggatctcgt tatgagcatg ggaaccagcg
      421 gaaccctctt cggatacagc gataccctcg tcgccgaccg ggaaggaagg ctctccgcgt
      481 tctgttcttc aaccggggga tatcttcccc tgctgtgcac caacaactgc acgctttcaa
      541 cggaagtgat tcggaatctg ttttcccgcg atgtgaaaac cctcgacatg ttggcaggca
      601 aggcaccgat cggttcccaa ggggtgacgg ttcttccctt cttcaccggc gagcgggttc
      661 cctatctgcc atatggaaag ggagtaatcg ctcatctgga ttcacagaac accaccgagg
      721 agaatatcgc ccgagcagcg ctggagagcg ccgtcttcgc tcttcggggc ggaatggata
      781 cgttcagtga actcggcttc accccttcgc gtctcatcct caccggagga ggggcaaact
      841 cgaagatctg gcgccaaatc gcaagcgacg tgttcaatct cgcggtcatt gttccctctt
      901 ccacggaaag tgccgcattg ggtgtggccc ttcaggcgct gtgggcgctc gagggcggtt
      961 cgatcacgga gatcgccaac gagcatgtgt tgttcgatga agataaaatg catcaacccg
     1021 accggatggc ggtcgaacaa tatgccgagg cgtacaggcg ctacagcacg tacgttcagg
     1081 cccttacacc catttttata aggagaatat gatgagttat tttgtcggtg aaaaagaata
     1141 tttcaaggga atcggaaaga ttcggttcga aggaaaagca tcaagaaacc ccctcgcatt
     1201 caagcattac aacgccggcg agcgggtcgg agataagacg atggcggagc acctgcgctt
     1261 ctccaccgcc tattggcact ccttctgtgc cgacggaagc gatccgttcg gcaagcccac
     1321 gatcgtcttc ccctttgact cgagcgatcc gtttgccaag gcggatgcgg cgttcgagtt
     1381 tttcaccaag ttgggaactc cgttctatgc gttccatgat gttgacgtgt tcaacgatat
     1441 cgatgatggc cgcgcatatg aaaagcgcta tcaggaggtt gccgacggct tgttggcccg
     1501 ccagaaagcc tccggcgtca agcttctttg gaacacgtcc aacgtgtttt cccacccccg
     1561 ctacatgaac ggggcggcga ccaaccccga cttcaacgtc gtcgcccggg ctgcggtcca
     1621 gatcaggaaa agcctggagg tgaatgttca gctcggtgga ttgaactacg tcttctgggg
     1681 tggacgggaa gggtatatga gcctgctgaa caccaatatg aagcgggagc aggaacacct
     1741 tgcccgcatg ctgatcatcg cccgcgatta cgcccggtcg atcggcttca ccggaaagtt
     1801 cctgatcgag cccaagccca tggagccgac caagcatcaa tacgactatg atgccgcaac
     1861 gacgatcggc ttcct
//
LOCUS       DAOPUZ010000269         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_67577_length_1875_cov_3.370330, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000269 DAOPUZ010000000
VERSION     DAOPUZ010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67577_length_1875_cov_3.370330"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..849)
                     /locus_tag="PKN79_04965"
     CDS             complement(235..849)
                     /locus_tag="PKN79_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNZ95147.1"
                     /translation="MNKEFINCDTIRDNTLKLAHHMYKNDGFIPDIIYASLRGGAYMA
                     NVFSEYYKMVKLNRKERPVFYAAVVARSYGYLRNQTRVMVDGWTYTPEFLRSGDKILL
                     VDDIFDTGKTVNALGEIIMQKGIPRNDLKIVVYDYKVPLYKKGEPLPIQPDYYVRKHV
                     LHSEEEEVWIHYNCHEFLGLSAKEIDEQFQDPEVRNILHEVRND"
     gene            complement(856..1611)
                     /gene="map"
                     /locus_tag="PKN79_04970"
     CDS             complement(856..1611)
                     /gene="map"
                     /locus_tag="PKN79_04970"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNZ95148.1"
                     /translation="MIQLKSEEQIKRIGEACTLTARLMEELETFIEAGMSTKDIDQYC
                     HDFIIRHKGIPSFLNYGGFPASACVSVNEEVIHGIPSTKKIIREGDLVSVDLGINLNG
                     YFSDMARTFIVGETKEEYRKLCEVTRKSLDLGIEAAGKNGARIQDIGKEVSRHCSQHG
                     YGVVRDFTGHGVGLAVHEAPEIPNYSAPFLPNPRIREGMVLAIEPMINLGTHKVSILE
                     DNWTVVTQDGLPSAHFEDTVAITRDGLVILTRG"
     gene            complement(1592..>1875)
                     /locus_tag="PKN79_04975"
     CDS             complement(1592..>1875)
                     /locus_tag="PKN79_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HNZ95149.1"
                     /translation="LEHLEEIDHLISRFSLNRPLDRIDYVDRNILRISVFSLLYANVH
                     PHVIIDEAVKLSQDFSSEVNYKFINGILDAMNKQVFEKREGMNYDSTEE"
BASE COUNT          404 a          468 c          493 g          510 t
ORIGIN      
        1 tgtcttgaag gatacgttca tcgtgagctc cctgcagttg gatggtgaga cgttcctggt
       61 cgccacgcag aagcacggga tgtattctgt tcaggttgac aaggccaacg tgaacaagaa
      121 tgacggcacg aatgttcaag cgatcagcaa ggcggaagaa tacgagttct gatgattcat
      181 tggttttcta aaggatagcc cccttctttt gtgtggaagg gggtttcgtt ttcatcagtc
      241 gtttcgcact tcgtggagaa tgtttcttac ctcgggatcc tggaactgct catcaatctc
      301 cttggcggag agaccgagga actcgtggca gttgtagtgg atccagactt cctcttcttc
      361 gctgtggaga acgtgcttgc ggacgtagta gtccggctgg atgggaaggg gttccccttt
      421 tttgtagagc ggaaccttgt agtcgtagac cacgattttc aggtcgttgc gggggatccc
      481 tttctgcatg atgatttccc cgagggcgtt gacggtcttc ccggtatcga agatgtcatc
      541 gacgaggagg atcttgtctc cgctgcgaag gaattcgggt gtatacgtcc acccgtcaac
      601 catgacccgg gtctgattcc tcaggtatcc gtaggatcgg gcgacgacgg cggcatagaa
      661 gaccggtcgc tcttttcggt tgagcttcac catcttgtag tattcgctga agacgttcgc
      721 catgtacgcc ccaccgcgca acgatgcata gatgatgtcg ggaataaagc catcattctt
      781 gtacatgtga tgcgcaagct ttagcgtatt gtctcgaatc gtgtcgcagt tgatgaattc
      841 cttgttcatg tgaacctacc ccctggtcag aatgactaag ccgtcccttg taatcgctac
      901 agtatcctca aaatgggcgg aaggcaaccc gtcttgggtg accaccgtcc agttgtcctc
      961 caggatcgag actttatggg ttccgagatt gatcatcggt tcgatcgcca acaccatccc
     1021 ctccctgatc cggggattcg ggaggaaggg ggcgctgtag ttgggaatct ccggtgcttc
     1081 atgaaccgca agaccgactc cgtggccggt gaagtcgcgg accactccat agccatgttg
     1141 agaacagtgc cgggagacct ctttaccgat atcctggatt cgagccccat tttttccggc
     1201 ggcctctatt cccaggtcga gcgatttccg ggtcacttcg cacagcttcc ggtactcttc
     1261 cttcgtctcg ccgacgatga atgtccgggc catatcgctg aaatatccgt tgagattgat
     1321 cccgaggtcg acagaaacca gatctccttc cctgatgatc tttttcgtgg agggtattcc
     1381 atgaatcact tcttcgttta ccgagacgca agcgcttgcg ggaaatccgc cgtagttcag
     1441 aaaggaagga attcccttat gccgaataat aaaatcgtgg cagtattggt cgatgtcttt
     1501 cgtgctcatt cccgcttcga tgaacgtttc caactcttcc atgaggcggg cggtcaaggt
     1561 acacgcttcg ccgattcgtt tgatctgttc ttcactcttc agttgaatca tagttcattc
     1621 cttcccgctt ttcgaacacc tgtttgttca tcgcatccag aattccgttg atgaacttgt
     1681 agtttacttc gctggagaaa tcctgactga gcttcaccgc ctcatcgatg atcacatggg
     1741 ggtggacatt tgcatacaga agggaaaata cgctgattcg aaggatgttg cgatcgacat
     1801 aatcaattcg atcaagagga cggttcaaac tgaatcgact gatcagatga tcgatctctt
     1861 caagatgttc aaggg
//
LOCUS       DAOPUZ010000270         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_68746_length_1855_cov_4.061667, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000270 DAOPUZ010000000
VERSION     DAOPUZ010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68746_length_1855_cov_4.061667"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PKN79_04980"
     CDS             complement(<1..585)
                     /locus_tag="PKN79_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HNZ95150.1"
                     /translation="MSTHTRFGYDFIKEPFLPEGKDEYYLRFAQNGKDPGTYRNLRSD
                     EIAILKSNLNHSPNWDNLLVRDPFDPLLIRNSEFYGLVRLGTVSVGTVSFHDFTVPVG
                     IVNSRIVSCDIGDSCSIMECTYLSHYIIDDDVVLYRIGEMQSTNHAKFGNGIVKDGED
                     PEVLITIDVVNEAKGRSIRPFDGMIPADAFLWSTY"
     gene            672..1745
                     /locus_tag="PKN79_04985"
     CDS             672..1745
                     /locus_tag="PKN79_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ95151.1"
                     /translation="MTEYDLPRRMKRLLKLAQRVSAWDPDELTPDRIAELNGKAHPSS
                     LLIRRLFGKPARNITTKTFVIPVNDGKVTGYLFEKEDSRGISDLLPLIIYYHGGGWVW
                     GNMDRYNIICAHLADITGATVLSVDYRLAPQHRFPTAVEDCYNTLLWAAAGCRYWKND
                     PDRIFLVGDSAGGNLAAVVSRLARDRKGPAIGGQVLIYPITDGRMRTASYAEYKDSPT
                     LTDRQMAFYIHNYQREPKDTLDPNFSPLLAKDHSRLPPTLIIGAEYDPLRDDGVLYAD
                     ALASADTPVKYLEVKKGLHGFFSYPKATGTDEAQSAITQFIRGKPVEQVALIGKKEWL
                     KAEKLELKKIKKQAKHYIETEIG"
BASE COUNT          440 a          542 c          486 g          387 t
ORIGIN      
        1 gtacgtcgac cacaaaaacg catcggcggg aatcattccg tcaaagggtc ggatgctccg
       61 acctttcgcc tcgttgacga catcgatggt gatgaggacc tcggggtctt ctccgtcctt
      121 cacgatgccg ttgccgaact tcgcgtggtt ggtgctctgc atctccccga tccgatagag
      181 cacaacgtca tcgtcgatga tgtaatggct taagtaggtg cactccatga tcgagcagga
      241 atctccgatg tcacagctga cgattcggga gttcacgatg ccgacgggaa cggtgaaatc
      301 gtgaaaactc accgtaccca ccgataccgt accgagccgg acgaggccgt agaactcact
      361 gtttcggatc aacagcggat caaagggatc tctgaccaaa agattgtccc agttcggact
      421 gtggttcaga ttgctcttga gaattgcgat ctcatcgctt ctcagatttc ggtatgtgcc
      481 cgggtctttc ccgttctgcg caaagcggag atagtactca tccttgccct cgggcaggaa
      541 cggctccttg atgaaatcat aaccaaatcg agtatgtgtg ctcatatgga aacagtatac
      601 tcccctcttt ccttccaata caaccgatga agcttcttga agagtccatt gagatagact
      661 acgatgaatt gatgactgag tatgacttgc ctcggcggat gaagcgattg ctgaagttgg
      721 cgcaacgggt ttccgcttgg gatcccgatg aactgacccc tgatcggata gccgagttaa
      781 acggcaaagc ccatccctcc tccctgttga tccgccggct cttcggaaag ccggctcgaa
      841 acatcaccac caagacgttc gtgattcccg tcaacgatgg aaaagtcacc ggctacctct
      901 ttgaaaaaga ggactcgcgg ggaatcagcg atctgctgcc cctgatcatc tactatcacg
      961 gcggcggctg ggtatggggg aacatggatc gctataacat catctgcgcc cacttggcgg
     1021 atatcaccgg agccacggtc ctttccgtcg actaccgcct tgctccgcag caccgttttc
     1081 cgaccgccgt tgaggattgc tacaacaccc ttctctgggc agccgccggc tgccgctatt
     1141 ggaagaacga ccccgatcgg atctttcttg tcggcgacag cgccggagga aacctcgccg
     1201 cggtggtcag ccgtctggcg cgggaccgca aaggtccggc gatcggaggc caggtcctga
     1261 tctatccgat caccgacggc cggatgcgaa ccgccagcta cgccgagtac aaggactccc
     1321 ccaccttgac cgatcgacag atggcgttct acatccacaa ctaccaacgc gaaccgaagg
     1381 ataccctcga cccgaacttt tccccgctgc tcgccaagga ccacagccgt cttcccccca
     1441 ccctgatcat cggggcggag tacgatccgc ttcgggatga cggcgttctg tacgccgatg
     1501 cgctcgcttc ggcggatacg ccggtcaaat accttgaggt gaagaaaggg ctccacggct
     1561 tcttttccta tccgaaagcg actggaacgg atgaggccca gagcgcgatc acccagttca
     1621 tcaggggaaa gcctgttgaa caagtggccc tcatcggaaa gaaagagtgg ctcaaagcgg
     1681 aaaagcttga gctgaagaag atcaagaagc aagcgaagca ctacatcgag accgagatag
     1741 gctagttctc taaagccacc ggcggaatca agggttgctg cagtgaagat acgcaaaacg
     1801 tctcaaaaca ccgttgaaat cctcaatccc atgcatatcg ccacgatcac ctata
//
LOCUS       DAOPUZ010000271         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_68750_length_1855_cov_3.308889, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000271 DAOPUZ010000000
VERSION     DAOPUZ010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68750_length_1855_cov_3.308889"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /gene="sbcB"
                     /locus_tag="PKN79_04990"
     CDS             <1..1177
                     /gene="sbcB"
                     /locus_tag="PKN79_04990"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HNZ95152.1"
                     /translation="IHRINAEFSRPDTTVAGFNSIRFDDEFIRNALYRNFFDPYRREY
                     EHNNSRWDIIDLVRATHDLRPEGIRWPERNEVNRPVFRLTSLTEANGIEHLGAHDALV
                     DVRATIAIARLIKEKQPRLYQYAYDLRSKNRVKRLLKAPFGGEHTSLILHTNPLFSSE
                     YGNTRLILPITYLKTNANAVVCFDLSCEVEPLLSATEETLMNTPGVFTISINKCPFVS
                     PVNVLTEELERKLGIDSALAATKAAYIRTHPNVIAVIHRVKDTFERIDDVDYQLYDAF
                     FTNRDQQVFETLRQAEPREKMGFLNQVQDPRFGTLLFRHVGRNWPEAFTEEERKRWIS
                     HAANRTLNPPGDGKMTWKFFYRSIEEKLESVDTTPREKQLLAELKEYGTALEAKIFG"
     gene            1222..>1855
                     /locus_tag="PKN79_04995"
     CDS             1222..>1855
                     /locus_tag="PKN79_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNZ95153.1"
                     /translation="MLEKLAEYQSQLEQIDLQLADGATMRDMNLYKKLTQERSHLQPI
                     VDELTRLKEIADQIADAKELLKEETDTEMIALTREELENLEAALEKTTSKVKLLLIPP
                     DPLAGKDIIMEIRAGTGGDEAALFAADLFRMYSHYADTRSWKMDILSSNETGIGGYKE
                     LIVSISGKDVYASLQYESGAHRVQRVPETESGGRIHTSAVTVAVLAEAEET"
BASE COUNT          471 a          546 c          486 g          352 t
ORIGIN      
        1 cattcacagg atcaacgccg agttcagcag gcccgacacc accgtcgccg gtttcaattc
       61 gatccgcttt gacgatgagt tcatccgcaa tgccctctat cgaaactttt tcgatcccta
      121 tcgacgggag tatgaacaca acaactcccg gtgggacatc atcgacctgg tccgtgcgac
      181 ccacgatctg agaccggaag gaatccggtg gcctgagcgc aatgaagtga accgccccgt
      241 gtttcgactc acttccttga ccgaagcgaa cggaatcgag catctcgggg cgcacgatgc
      301 cctggttgac gtccgagcta cgatcgcaat cgctcgtctc atcaaggaaa aacagccaag
      361 gctctatcag tatgcctatg acctgcgctc aaagaaccga gtgaaaagac tgctgaaagc
      421 cccctttggc ggggagcaca cctccctgat tctgcacacc aaccccctct tctcctcgga
      481 atatggcaac acccgcctca ttctgccgat cacctacctc aaaacgaacg ccaatgcggt
      541 cgtgtgcttc gatctgagct gcgaagtgga accgctcctc tcagcgacgg aagagacgct
      601 gatgaatacg ccgggagtct ttacgatttc gatcaacaaa tgcccgttcg tctcaccggt
      661 gaacgtgctt accgaggagc tggaacggaa gcttgggatc gattccgccc tcgctgcgac
      721 gaaagccgcc tatatcagga cgcacccgaa cgtgatagcc gtgatccacc gggtgaaaga
      781 cactttcgag cggatcgacg acgtcgatta ccagctctac gacgcgttct tcaccaacag
      841 ggaccagcag gtctttgaga cccttcgcca agcggagccg agggaaaaaa tgggctttct
      901 caatcaggtt caggatcccc ggttcggaac tctcctcttc cgccatgtcg ggcggaattg
      961 gccggaggcg ttcaccgaag aggaacggaa acgttggatc agccacgctg ccaatcgcac
     1021 cctcaatcca ccgggcgacg gcaaaatgac gtggaagttc ttctatcgga gcatcgaaga
     1081 gaaactcgag tcggtcgaca ccacccccag ggagaaacag ctccttgccg agttgaaaga
     1141 gtacggcacc gctcttgagg caaagatttt cgggtaatac cccagctatg caaagcaaac
     1201 ctccttttgt atacttcctc tatgcttgag aagttagccg aataccaaag ccaattggaa
     1261 cagatcgatc ttcaactcgc cgacggtgcg acgatgagag acatgaacct ctacaagaaa
     1321 ctcacccagg aacgcagcca ccttcagccg atcgtcgatg agctgacccg actaaaggag
     1381 atcgccgatc agattgccga cgcgaaagaa ctgctgaaag aggagacgga caccgagatg
     1441 atcgccctga cccgcgagga gcttgagaat cttgaagctg cattggagaa aacaacctcc
     1501 aaagtaaaac tgcttctgat tccccccgat ccgctggcgg gaaaagacat catcatggag
     1561 attcgggcag gcaccggcgg agacgaagcg gctctcttcg ccgccgatct gtttcggatg
     1621 tattcgcatt atgccgacac tcgctcctgg aagatggata tcctctcctc caacgagacg
     1681 ggaatcggcg gatacaagga actgatcgtt tcgatcagcg ggaaggacgt atacgcatcg
     1741 cttcagtatg aaagcggcgc ccaccgcgtt cagcgcgttc cggaaacgga aagcggagga
     1801 agaatccata cctcggcggt caccgtcgcg gtgctcgctg aagcggaaga gaccg
//
LOCUS       DAOPUZ010000272         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_68981_length_1851_cov_3.396993, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000272 DAOPUZ010000000
VERSION     DAOPUZ010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68981_length_1851_cov_3.396993"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..887
                     /locus_tag="PKN79_05000"
     CDS             126..887
                     /locus_tag="PKN79_05000"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HNZ95154.1"
                     /translation="MSGATEIYYNELMRFFGLIKQNEADSIDRAAALLVQEIKDDRII
                     NVIGTGGHSNIGAWEMFYRAGGLRCINAILDPGTSLEPGALRSTIIERSLGYGTAVLT
                     AYEVNEGVLIIVNAYGLNTMTIEVALEARKREIKTIGVTSRTFSESVPKDHPARHPSK
                     QNLCDVVDVHIDTHMPVMDAIVPIDGLLAPVGPTSTILNAFCLNMLVVRTVELCMDQG
                     IEPPVNISANVAFTPEMTEYSRRMMEKYKHRLYHY"
     gene            902..1723
                     /locus_tag="PKN79_05005"
     CDS             902..1723
                     /locus_tag="PKN79_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNZ95155.1"
                     /translation="MASNIRVLVFGAHPDDDDLQCGGTAIKLSKAGHTVKFVSCTNGG
                     TGHHEIGGIELVRRRYEEAQRAAKVAGIAEYQVLDIHNGELEASVMNRKTIIRIIREF
                     KPDLIITHRPYDYHPDHRITSQLVQDASYIMSVPNMLPLTEAMTEFPVICYMSDTFQK
                     PIPFSPDIVIGIDDVFDRKVEMIHSHTSQMYEWLPYNRGVLHTVPTGDEERKEWLREH
                     FLDPRDRADRYRNRLIELYGEAEGKAIRYAEAFEVCEYGRPLALTRTEIENVFVL"
BASE COUNT          471 a          515 c          478 g          387 t
ORIGIN      
        1 gagaacagca agaatcatca gctcagccct ctacgccatg gagattctta tattctggat
       61 acccgcctca aatacacttt gaccttcccc atttgggatg atagattgaa caaggaggtg
      121 atctcatgag tggtgcaact gagatttatt acaacgaact tatgcgattt ttcggcttga
      181 taaagcagaa cgaggccgat tccatcgata gagccgccgc gctgttggtt caggagatca
      241 aggatgatcg gatcatcaac gtcatcggaa ccggcggaca ttccaacatt ggagcatggg
      301 agatgttcta tcgggcagga ggacttcgat gcatcaacgc catcctcgac ccaggtacct
      361 cattggagcc cggagcgctt cgctccacga tcatcgaacg ctccttaggc tatggtacgg
      421 cggtccttac cgcctatgag gtgaacgaag gtgtgctcat catcgtcaac gcgtacggct
      481 tgaacaccat gacgatcgag gtcgccttgg aagctcgaaa aagagaaatc aagacgattg
      541 gggtgaccag cagaacgttc tccgagtcgg taccgaagga tcatcccgcc cgccatccga
      601 gcaaacagaa cctctgtgat gtcgtcgatg tccacatcga tacccatatg ccggtcatgg
      661 atgcgattgt tccgatcgac ggccttctcg cgccggtagg accgacctcg acgatcctca
      721 atgcattctg cttgaatatg ctcgtcgtcc gaaccgtcga actatgcatg gatcagggaa
      781 tcgagcctcc ggtgaacatc agcgccaacg tcgcatttac tcccgaaatg acggagtata
      841 gccgccggat gatggaaaaa tacaaacaca ggctctatca ctactaaaag aggagtacat
      901 catggcttca aacattcgcg tactggtctt cggggctcat cccgatgacg atgacctcca
      961 atgcggcggc accgcgataa aactcagcaa ggccggacat acggtaaagt tcgtctcctg
     1021 caccaacggg ggaaccgggc atcatgaaat cggcggaatc gaacttgtcc gcaggagata
     1081 tgaagaggcc cagagggccg caaaagtcgc cggtatcgcc gaatatcagg tgctggatat
     1141 ccacaacggc gagcttgagg cttccgttat gaacagaaag acgatcatca ggatcatcag
     1201 ggagttcaag cccgacctca tcatcaccca ccgcccgtat gactaccatc ccgaccaccg
     1261 catcacctcc caactcgtcc aggacgcgtc gtacatcatg agcgtcccca atatgcttcc
     1321 cctcaccgag gcgatgaccg agttcccggt gatctgctat atgagcgaca cgttccaaaa
     1381 accgattccg ttcagccccg acatcgtcat cgggattgat gacgtcttcg accggaaagt
     1441 cgagatgatc cactctcata cttcccagat gtatgaatgg ctcccctaca atcgcggagt
     1501 gctccacacc gttccgaccg gcgatgagga gcgaaaggaa tggctcagag agcactttct
     1561 ggatccccgg gatcgggccg accggtatcg gaaccgcttg attgagctct atggcgaagc
     1621 tgaaggaaag gccatccggt atgccgaagc gtttgaagtc tgtgaatacg gccggccgct
     1681 tgccttgact cgcaccgaga ttgaaaacgt ttttgtcctc tagattcgaa aaggaaaccc
     1741 gcaatgagca tctttgaaac gatcatgctg gcctgcttcg gcatggcatg gccggtgaat
     1801 atcatgaaaa cagtcaggtc caaaaccgcc cggggccgga gtctgatgtt c
//
LOCUS       DAOPUZ010000273         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_68996_length_1851_cov_2.894766, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000273 DAOPUZ010000000
VERSION     DAOPUZ010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68996_length_1851_cov_2.894766"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKN79_05010"
     CDS             complement(<1..375)
                     /locus_tag="PKN79_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ95156.1"
                     /translation="MKKALVIVLSLVFAMGVVFAAGAAEKAAVPKIGIAVPSPDHGWT
                     GGIGWWADKAVDELQKQYPGKYEFRVLHADGYAKQISDVEDLMVWGMDYLVILPHESA
                     PLTPVVKEAHAAGVKCIVVDRGL"
     gene            complement(390..1364)
                     /locus_tag="PKN79_05015"
     CDS             complement(390..1364)
                     /locus_tag="PKN79_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ95157.1"
                     /translation="MAEHKIKEYIKNYRIKDHSLGMAFIALFVIATIVGWPNFLKVRN
                     LTNILRQVSYTGIIGLGMTFVIISGGIDLSVGSMAAFVGGVSIFFLNLFGPDSTLALV
                     LTFFFALVFGSLLGALNGVMVAKFRMAPFIVTLGTMSIFRSLIQYFSNAGTILSVNMG
                     FGKLGSSIFLGLPVPVWFFIIIGVSLHIILNYTSFGRYLCATGANEQVARYSAIKVSL
                     VKFVPYVITGFTVGVTAIMWSSRLNCINPSDCAGYEMDAIAAAVIGGTLMSGGKGSII
                     GTMMGAVMLGIINNMLVMGGISAFLQQAVRGFVIIVAVLLQYNSDSNK"
     gene            complement(1357..>1851)
                     /locus_tag="PKN79_05020"
     CDS             complement(1357..>1851)
                     /locus_tag="PKN79_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ95158.1"
                     /translation="KGIVMDYDIPKNITLISLKEKYVKRGLIDKHSEEKSGKEYVKGF
                     NIVAASHKAPLRFFSGGNQQKVYLARWIDTKPRVLILDEPTRGIDVNAKREIYEFIHH
                     LADEGISCIVISSEMEEVIGMCDRVYVMREGRIAGHLEKEHITEENIVFNATGIQKGV
                     KRDG"
BASE COUNT          437 a          524 c          452 g          438 t
ORIGIN      
        1 caaaccccgg tcaacgacga tgcacttgac gccggcagcg tgtgcttctt tcacaaccgg
       61 agtcagcgga gcgctttcgt ggggaagaat gacaagataa tccatccccc agaccatcag
      121 gtcctcaaca tcggagatct gctttgcata cccgtcggcg tgcaagaccc ggaactcata
      181 cttgccggga tactgcttct gaagctcatc gaccgctttg tcggcccacc agccgattcc
      241 gccggtccag ccgtggtcgg gagaaggtac tgcgattccg atcttcggaa ctgcggcttt
      301 ctcggctgca ccagcggcga agacgactcc catggcaaat accaaagaga gaacgattac
      361 taaagctttt ttcatacata gcctcctgtt tatttgttac tgtcgctgtt gtactgaagc
      421 agtactgcga caataattac aaaacccctg actgcttgtt gcaggaaagc gctgatgcct
      481 cccatgacaa gcatgttgtt gataatgccg agcatcactg cccccatcat cgtaccgatg
      541 atgctcccct tccccccgct catcagcgtt ccgccgatga ccgccgcggc aattgcgtcc
      601 atctcgtagc cggcgcaatc gctggggttg atgcagttga gcctgcttga ccacatgatc
      661 gccgtgacac cgacggtgaa tccagtgatc acgtacggga cgaatttcac gagagagact
      721 ttgatcgccg agtatcgggc aacctgctcg tttgcaccgg tcgcgcagag ataccggccg
      781 aagctcgtgt agttgagaat gatatggagt gaaaccccga tgatgatgaa aaaccatacg
      841 ggcaccggaa gaccgagaaa gatgctgctt ccaagctttc caaaccccat attcactgaa
      901 agaatcgttc cggcgtttga aaagtattgg atgagcgatc tgaagatcga catcgtaccc
      961 agcgttacga tgaacggggc cattctgaac tttgcaacca tcactccgtt cagagctccc
     1021 aagagggatc cgaagaccag agcgaagaaa aacgtgagga ccagcgcaag cgtcgaatcg
     1081 gggccgaaca ggttgaggaa gaagattgaa actcccccca cgaaggccgc catcgacccc
     1141 accgagagat cgatgccgcc gctgatgatg acgaatgtca tccccaggcc gatgattccc
     1201 gtataggaaa cttgccgaag aatgttggtc aggtttcgaa ccttgagaaa gttcggccaa
     1261 ccgacgatgg ttgcaatcac gaacaaagcg atgaacgcca ttcccagcga gtggtctttg
     1321 atccgatagt tcttaatgta ttctttgatc ttatgctcag ccatctctct tgaccccttt
     1381 ttgtatcccg gttgcattga atacgatatt ctcctcggtg atgtgctcct tctccagatg
     1441 cccggcgatt cttccttccc gcatcacgta cacccggtcg cacatcccga tcacttcctc
     1501 catttcgctg ctgatcacga tgcaactgat tccttcatca gcaagatggt ggatgaattc
     1561 atagatctcc cgtttcgcat tgacatcgat gccgcgagtg ggctcatcaa gaatgagaac
     1621 ccggggtttc gtgtcgatcc accgcgccag gtacactttc tgctggtttc ccccactgaa
     1681 gaaacggaga ggcgctttgt gcgaagccgc gacgatgttg aaccctttga cgtattcctt
     1741 gccgctcttt tcctcactgt gcttatcgat caaacctcgt ttcacgtact tctctttgag
     1801 cgaaatcagg gtgatattct tgggaatgtc atagtccatg acgattccct t
//
LOCUS       DAOPUZ010000274         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_69284_length_1846_cov_3.185371, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000274 DAOPUZ010000000
VERSION     DAOPUZ010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69284_length_1846_cov_3.185371"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKN79_05025"
     CDS             <1..390
                     /locus_tag="PKN79_05025"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNZ95159.1"
                     /translation="ATRMMNPTATEEQIVAAVAEIVTIAGGKPCPPIFLGIGLGGTME
                     RAAYLSKRALLREVGVPHSVERYAKLEEKILAGVQATGVGAGGLGGKITALNVAIEYE
                     PTHIAGLPMGVSINCWADRIAHIVWEG"
     gene            393..911
                     /locus_tag="PKN79_05030"
     CDS             393..911
                     /locus_tag="PKN79_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HNZ95160.1"
                     /translation="MRTLHLPISEEDLQSLRAYDAVSFSGTLYVGRDQVHQRLARLLE
                     EGKPLPVELEGQAIYYMGPSPPLPGMIMGSCGPTTSARMDPFTPQLLGKGLKVLIGKG
                     PRSKAVHDALKKEKAVYVQAFGGCGALYAKTIVSVEPVAFEDLGPEALLRLEVRDFPA
                     IVAIDSHGGSVF"
     gene            1053..1832
                     /locus_tag="PKN79_05035"
     CDS             1053..1832
                     /locus_tag="PKN79_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HNZ95161.1"
                     /translation="MLRKIIFAITPVSLIFLLAGCPILIEPSVLDDVLPSVAQMISDE
                     GGDLYTFSVSDLPTPSTLEERFSYTYGYMLYNTLLQQDEIANLNAPYFAKGALDANAG
                     SAIFTADEMNRILFEVQLMLLQIAQQELEALSKANYEYAHAFLEENKTREGIITTESG
                     LQYTVIKPSDGPHMDPSLSPRVRFTLTDGQGEIVDSAESRLLSLANLTPGFIEGLLLM
                     NVGGRYRFWLAPDLTYPDGGESNPAGPNALLIADVTLLGLE"
BASE COUNT          381 a          521 c          550 g          394 t
ORIGIN      
        1 gccacccgga tgatgaatcc caccgccacc gaagagcaga tcgtagcagc ggtcgccgaa
       61 atcgtcacga tcgccggagg gaagccctgc cccccgatct tcctcggaat cgggctgggc
      121 ggtacgatgg agcgcgccgc ctacctcagc aagcgggcgc tccttaggga ggtgggggtg
      181 ccccacagcg tggagcggta tgcgaagctt gaagagaaga tccttgccgg ggtccaggcg
      241 accggggtcg gtgccggagg cctcggagga aagatcaccg cgttgaacgt ggcgattgag
      301 tatgaaccca cccatatcgc aggcttgccg atgggggttt caatcaactg ctgggcggat
      361 cggatcgccc atatcgtctg ggagggctga ctatgagaac tttacacctt ccgatcagtg
      421 aagaagatct gcaatcgctt cgggcatatg atgcagtgag cttttcgggt acgttgtatg
      481 tcggtcgcga ccaagtgcat caacggctgg cgcgactgct tgaagagggg aagcccctgc
      541 cggtggagct tgaggggcag gcaatctatt atatgggacc gtcacccccg ctgccgggga
      601 tgatcatggg ctcctgcggt ccgacgacga gcgcccgcat ggatcccttc accccgcagc
      661 tgcttggaaa ggggttgaag gtcctgatcg gaaaaggtcc tcgatccaag gcggtccatg
      721 acgctcttaa aaaagagaag gcggtctatg tgcaggcgtt cggcggctgc ggcgccctct
      781 acgccaaaac gatcgtcagc gtcgagccgg tggcgttcga agatctgggg ccggaggcgc
      841 ttctgcggct ggaagtcagg gactttcccg cgatcgtcgc catcgattcc cacggagggt
      901 cggtattctg atgcaatcga ggggattttt ctggatttat ccccccaagt gcgatacagt
      961 ttctgcatgc aggttgatta ccgcccatcc tcgtggggtg aagggtcggc ggtgatcaaa
     1021 atggtcgtat ccgtccgatg aaaggattca gagtgctgcg aaagataatc tttgcaatca
     1081 ccccggtgag tttgatcttt ctcctcgcag ggtgtccgat tctcatagag ccgtctgttt
     1141 tggatgatgt tcttcccagt gtcgctcaga tgatcagcga tgaaggcggg gatttgtata
     1201 cgttttccgt cagcgatctg ccgacccctt ccacattgga agagcgtttc agctacacct
     1261 atgggtatat gctttacaac accctgcttc agcaggacga gattgccaat ctgaacgccc
     1321 cgtatttcgc caaaggggcg ttggatgcaa acgccggttc agcgatcttc accgccgacg
     1381 agatgaatcg gatcctgttt gaagttcaac tgatgctcct gcaaatcgcc cagcaggagc
     1441 ttgaagccct cagcaaagcc aattatgaat acgcccatgc gttcttggaa gagaataaga
     1501 cccgcgaggg aatcatcact accgaaagcg gtcttcagta tacggtcatc aagccgagcg
     1561 acggacctca catggatccc tccctcagtc cccgagtcag gttcaccctc accgacggac
     1621 agggggagat cgtcgattcc gctgagagtc ggctcctctc cctcgccaac ctcactcccg
     1681 ggtttatcga ggggctcctc ttgatgaacg taggggggcg ttaccgcttc tggctcgccc
     1741 ccgatctgac ctatcccgac ggcggggaat ccaaccccgc cggtcccaac gccctgctaa
     1801 ttgccgacgt taccttgctt gggctcgagt gagaatacgt acatcg
//
LOCUS       DAOPUZ010000275         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_69480_length_1842_cov_4.839955, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000275 DAOPUZ010000000
VERSION     DAOPUZ010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69480_length_1842_cov_4.839955"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PKN79_05040"
     CDS             <1..1272
                     /locus_tag="PKN79_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNZ95162.1"
                     /translation="LFEAVPVQEIEDVVKESITGQYARRIWFLYEWLMDRRLSLDDVT
                     QGNYIPALDPKQQYAVAGIRSPRHRVVDNLPGTPAFCPLVTKTPELLEFESLALQDRA
                     STLVSTIPKEILSRAAAFLLLKDSRASFTIEHESPTPDRLARWATAIGQAGRVSLSKE
                     ELLRLQELVIGESVFIKPGFRTEGGFVGEHEPMTGFPIVEHASSRSEDVIPLVEGILA
                     YEQRTRDSFDPVVSAASVAFGFVQVHPFSDGNGRIHRYLIHHILAQRGFNPPGIIFPV
                     SHAMLKRAQEYQRVLRVYSSSILPFIEWTVTPDYNIHVLNETADYYRYFDATEYTLFL
                     YRCVQDTIEDGFEQEVSHIIAYDRFQAGLQRLGEMPDRSVQLLYQFLRQHNGTLPKRA
                     KEKEFKELSVQMIKEIEAIYAEAFGTGSSLE"
     gene            1330..>1842
                     /locus_tag="PKN79_05045"
     CDS             1330..>1842
                     /locus_tag="PKN79_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNZ95163.1"
                     /translation="MNVLITGATSGIGFETALLFAKNGAHVWGASRSPIASPSDRIVF
                     RRMDVTNEESVKKSVEEIWNEAVKTTGKGIDVVVHSAGYGIGGSAEDTPIDDAIAQFD
                     TNYFGVLRVNRVLLPRMRAQGSGRVVILGSIAGRISIPFQSHYSATKFALEAYTEALR
                     LEGGPFGLTAT"
BASE COUNT          437 a          501 c          486 g          418 t
ORIGIN      
        1 ctctttgaag cggttcctgt tcaagagatt gaagatgtcg tcaaagagtc catcaccggc
       61 caatacgccc gtcgaatctg gtttctctat gagtggttga tggatcgcag gctctcattg
      121 gatgacgtga ctcagggcaa ctatatcccg gctcttgatc cgaaacaaca atatgcggtc
      181 gccggtatcc gctcaccgcg gcatcgagtg gtcgacaatc ttccgggaac tcccgctttt
      241 tgtccgcttg taacaaaaac acctgagctg ctggaatttg aatcgcttgc cttgcaagat
      301 cgtgcctcga ctctcgtatc gacgatacca aaggagattc tatcaagagc agccgcattt
      361 cttttgttaa aagattcacg agccagtttt acgatcgaac atgaatctcc caccccggat
      421 cgacttgcac gatgggcgac ggccatcggg caggcgggtc gtgtttcact gagtaaagaa
      481 gagctccttc ggcttcagga gcttgtcatc ggagaatcgg ttttcatcaa gccgggtttc
      541 cgaaccgaag gcgggtttgt cggagaacat gaaccgatga cgggattccc gatcgtcgaa
      601 catgcgagca gccgaagtga agacgtgatc cctttagtgg agggcattct tgcatatgaa
      661 caacggaccc gagattcctt tgaccccgtc gtgagtgctg caagcgtggc attcgggttt
      721 gtccaagttc acccgttcag cgacggcaac ggaaggattc atcgctatct gattcaccat
      781 atcctggctc agcgaggctt caatccaccc ggaatcatct ttcccgtctc ccatgcgatg
      841 ctgaaacggg cacaggagta tcagcgggtt ctacgagtct actcctcctc gattctgccg
      901 ttcatcgagt ggaccgtcac ccccgattac aacattcacg ttctcaacga aaccgccgac
      961 tactaccgct acttcgatgc caccgaatac actctcttcc tctatcggtg tgtgcaggat
     1021 acgatcgagg atggttttga gcaagaagta tcgcacatca tcgcttatga ccgctttcaa
     1081 gcaggtcttc agcgccttgg cgagatgccg gatcgaagcg ttcagctcct ctaccaattc
     1141 ttgagacaac acaacggaac gcttccaaag cgggctaaag agaaggagtt caaagagctt
     1201 tccgttcaga tgattaagga aatagaagcg atctatgccg aagcgttcgg aaccggctct
     1261 tccttggaat gaagatgtgg taggatggtt cataaagaaa atgaatcttt ctatcttgga
     1321 gcatgaatga tgaacgttct tatcaccgga gcgacctccg gcatcggctt tgaaaccgct
     1381 cttctctttg caaagaatgg agctcacgtc tggggagcga gccggagtcc catcgcctct
     1441 cccagcgatc ggatcgtctt cagacggatg gatgtcacaa acgaggagtc ggtaaagaaa
     1501 agcgtcgaag agatctggaa cgaagcggtg aagacgacgg ggaaaggaat cgacgtcgtc
     1561 gtccactccg ccggatacgg aatcggcggc agcgccgagg acaccccgat tgatgatgcg
     1621 atcgcccagt tcgacaccaa ctacttcgga gtcctccgag tcaaccgggt tctccttccc
     1681 cgcatgcgag cgcagggaag cggcagggtc gttatcctcg gctcgatcgc gggacgaatc
     1741 agcatcccct ttcaaagcca ttacagcgcc acgaagttcg ccctggaggc gtacaccgaa
     1801 gcgttgcggc ttgaaggcgg gccattcggc cttaccgcca cg
//
LOCUS       DAOPUZ010000276         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_69566_length_1841_cov_3.369541, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000276 DAOPUZ010000000
VERSION     DAOPUZ010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69566_length_1841_cov_3.369541"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PKN79_05050"
     CDS             <1..1137
                     /locus_tag="PKN79_05050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ95164.1"
                     /translation="KEATVGNLVGGLDQLSKARKVTRVTGTGTFRDEKTLLVKTSDGD
                     LLYTFNDLILAVGSRPAHIPGIPYEDERIWDSTKALELSEVPKHLLIIGGGIIGMEMA
                     RIYHGLGSRITIVEMADEIIPPADRDVRQVLLKSVKSKYAIHTKTKVGKVEAHEKTLK
                     VFLEGNETSPIEADAVLIAVGRKANSDLIGIENTSIAVNKRGWIEVDDQMKTSVEHIY
                     AIGDVTGDPMLAHRASYQAKVAAEVASGKKSGFTPLGIPSVAYTSPELAWVGLTENEA
                     REKGIAYRKGVFPWQASGRALSAMAPNGLSKILFDEETGRVLGAAIAGKNAGELIGEA
                     VLALEMGAVALDLGLSIHPHPTLNESLSMAAQLAEGIATDLMNR"
     gene            1238..>1841
                     /locus_tag="PKN79_05055"
     CDS             1238..>1841
                     /locus_tag="PKN79_05055"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNZ95165.1"
                     /translation="MNISVLQEARYGYKPKLPTILTSGACKLVPVFGKKTCSAGDSEA
                     IASLFPNTYGAPIVSYAQGENNGAKTVRNVGVILSGGQAPGGHNVISGLFDAMQAANP
                     ENKLYGFKGGPSGILKDDYRIIDAEYLSAFRNTGGFDIIGSGRTKLETVEQFETCARV
                     CKKHEIGALIIIGGDDSNTNAAVLAEYFLEKNHGIQVLGCP"
BASE COUNT          425 a          505 c          570 g          341 t
ORIGIN      
        1 aaggaagcca cggtaggcaa cctcgtcgga ggtttggatc agctttcgaa ggcgcggaag
       61 gtcacgcgcg tgaccggaac aggaacgttc agggatgaga aaacgctact cgtgaagacg
      121 agcgacgggg atcttctcta caccttcaac gatctgattc tcgcagtcgg ttcgcggccg
      181 gcacacatac cgggaatccc ctatgaggat gagcggatct gggactccac caaggccctt
      241 gagcttagcg aggtgccaaa gcatctgctc attatcggcg gggggatcat cgggatggag
      301 atggcccgga tttaccacgg gttgggaagc aggatcacga tcgttgagat ggcggatgag
      361 atcatccctc cggcggatcg ggatgtgcgg caggttctct taaagagcgt gaagtccaag
      421 tatgcgatcc ataccaagac gaaggtcggc aaggtcgaag cacacgaaaa aaccctcaag
      481 gtctttctgg agggcaatga aacctctccg atcgaagcgg atgccgtcct gatcgcggtc
      541 ggccgaaagg cgaacagcga cctcatcgga atcgagaaca cctcgattgc cgtgaacaag
      601 cgcggttgga tcgaggtcga tgaccaaatg aagaccagcg tcgagcacat ctatgcgatc
      661 ggggacgtga ccggcgaccc gatgcttgcc caccgggcca gctatcaggc caaggtggcg
      721 gccgaggtgg caagcggaaa gaagagcggc ttcaccccgc tgggcattcc ctcggtcgcc
      781 tatacgagcc ccgagcttgc gtgggtcggt ctaacggaga acgaggctag ggagaaggga
      841 atcgcctaca ggaagggggt cttcccctgg caggccagcg gccgggccct cagcgcgatg
      901 gccccaaacg gcttgtccaa gatcctcttt gatgaggaga cgggccgggt tctcggggca
      961 gcgatcgccg gaaagaacgc cggcgagctg atcggggaag cggtcctcgc tctggagatg
     1021 ggggcggtcg ctctggattt gggcctttcc atccatcccc atccgacgct caacgagtcg
     1081 ctcagcatgg ccgcccaact ggccgaggga atcgcgacgg atctgatgaa tcgctagatg
     1141 tacatggatg cgcctttgga acaccgctct tgaactggag cggtgtttta ttctaatgtt
     1201 catgaagtga aagatttctt ttcaaggagt accatctatg aacatttcgg ttttgcagga
     1261 agcccgctac ggctataagc ccaagttgcc gacgatcctt acaagcggcg cgtgcaagct
     1321 ggttcccgtt tttggcaaga agacatgcag cgccggtgac agcgaggcga ttgcctccct
     1381 ctttcccaat acctacggcg caccgatcgt cagctacgcg caaggcgaga acaacggagc
     1441 aaaaaccgtt cgcaacgtcg gagtgatcct ctccggcgga caggctcccg gcggacacaa
     1501 cgtgatcagc ggactctttg atgcgatgca ggcggcgaac cccgaaaaca agctctacgg
     1561 cttcaagggc ggtccgtcgg gaatcctcaa ggatgattat cggatcatcg acgctgaata
     1621 tctttctgcc ttcagaaaca ccggtggttt cgacatcatc ggcagtggcc ggaccaagct
     1681 ggaaacggtc gagcagttcg agacctgcgc cagagtgtgc aagaagcatg agatcggcgc
     1741 gctcatcatc atcggcggag acgactcgaa caccaacgcc gcggtattgg ccgaatactt
     1801 tctggagaag aaccacggca tccaggtgct tggctgtccc a
//
LOCUS       DAOPUZ010000277         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_69699_length_1839_cov_2.539798, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000277 DAOPUZ010000000
VERSION     DAOPUZ010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69699_length_1839_cov_2.539798"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PKN79_05060"
     CDS             complement(<1..576)
                     /locus_tag="PKN79_05060"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNZ95166.1"
                     /translation="MRARLGKAREEAVTEALLRAPTLVDAIAALRPTRYAALARSYDQ
                     SGDIQSIELVLLKMEIALYREIAQYLDGVEGRFVAELLGKIEIDNLKNSIRLWYSSII
                     HRRPIRHRSEYLFKEVILHPVDWTALINALSWDGVVEATANTPYHPILPEYTEGSLRE
                     EGLFQLESRLDRVWYEEIRKATAWLKKTDRQK"
     gene            complement(615..941)
                     /locus_tag="PKN79_05065"
     CDS             complement(615..941)
                     /locus_tag="PKN79_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95167.1"
                     /translation="MRSDIINEVLTVEDRAQQIVRDAEREAREIITNAQTEANAFVRD
                     ALKQDRILAQETIAQAEADLVDQLTEYEASLDRSSRLTAEEVKEIAQAIVIEVCKTNL
                     DGISES"
     gene            complement(1021..1650)
                     /locus_tag="PKN79_05070"
     CDS             complement(1021..1650)
                     /locus_tag="PKN79_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95168.1"
                     /translation="MQPAFTVSGVLIQKDGTPYIQYSSPIYRITTNEEGEEKHDRDDT
                     LYLFTDQDGRFILNEVKAGSYLFDLKVDDDWYGVRFDVPEQDAKTMGINTILLLESYQ
                     VDDPAFEGRVSYFDKVAVLLEAQIEDAFHVEVADEYDARLTLNVIEQTDEESFWNAIF
                     PSFDEDPFFGFDDGFVTDDDFYFDPLVFDEMIGDWESPPTEQTVTTAAP"
     gene            1649..>1839
                     /locus_tag="PKN79_05075"
     CDS             1649..>1839
                     /locus_tag="PKN79_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95169.1"
                     /translation="MLILTTKLSLPRGESEYTKELPVENSVGPLEKTATVLVVLVESP
                     LYNALPKGRLGTAIGYLNN"
BASE COUNT          413 a          513 c          457 g          456 t
ORIGIN      
        1 tttttgacga tcggttttct tcagccaagc ggtcgccttg cgaatctctt cataccacac
       61 ccggtcgagc cggctctcca attgaaacag cccctcttcc cgcaaagacc cttccgtata
      121 ctccggaaga atcggatggt agggggtgtt tgccgtggcc tctaccaccc catcccatga
      181 gagcgcattg atgagcgcag tccaatcgac cggatgaaga atcacctcct tgaagaggta
      241 ttcactccga tgacgaatcg gacgacggtg gatgatcgac gaataccaga gtcgaatcga
      301 attcttcagg ttgtcgatct cgattttccc aagcagttcc gctacgaacc gtccctccac
      361 tccatccaga tattgggcga tttcccgata cagggcgatc tccatcttga gcagaaccag
      421 ctcgatggat tggatatccc cgctttgatc atacgaacgg gccaaggcgg catatcgggt
      481 cggtcttaag gcggcaatcg catccaccag ggtcggagca cgcaaaagag cctcggtaac
      541 cgcttcttcc cgcgctttac cgagtcgggc acgcatcttc gcattgacaa atccgtacgt
      601 atctattgag ctcattatga ctcgcttatt ccatccaaat tggttttaca cacctcgatg
      661 acgatcgcct gggcgatctc tttcacttct tcggcggtaa gtcgggaaga gcggtccaat
      721 gaggcttcat attcggtcaa ttggtcgacc aggtccgctt cggcttgagc aatcgtttct
      781 tgggccagga ttcgatcttg tttcagcgca tcccggacaa atgcattcgc ttccgtctgg
      841 gcattggtga tgatttcacg agcttcccgc tccgcatctc ggactatttg ctgagcccga
      901 tcttcaaccg ttaatacttc gttgataata tcactgcgca cgtgccaacc tcccaccctt
      961 gcaactcgat atgctttctc tcatatgttc ttctttttgt tcaaaggtgt caagcaaaca
     1021 ttagggcgca gcggtggtga ctgtttgttc tgtgggaggt gattcccaat caccgatcat
     1081 ctcgtcaaag accagcggat cgaagtagaa gtcatcgtcc gtcacgaagc cgtcgtcgaa
     1141 gccgaagaac gggtcctcat cgaatgacgg gaagatcgca ttccagaagc tctcctcatc
     1201 cgtttgctcg atcacgttga gggtcagacg ggcatcatac tcatccgcca cttcgacatg
     1261 gaaggcatct tcaatctgag cctcaagaag tacggcgacc ttatcgaaat agctgactcg
     1321 accttcaaag gcggggtcgt ccacctgata ggactcaaga agcaatatgg tgttgattcc
     1381 catcgtcttg gcatcctgtt cgggcacatc gaagcggact ccataccaat catcatccac
     1441 cttcaaatca aagaggtagg atccggcttt cacctcattg aggatgaacc gtccatcctg
     1501 gtcggtgaag aggtagagcg tatcgtcccg atcatgtttc tcctcgccct cctcgttggt
     1561 cgtgatccga tatatcggcg atgagtattg gatgtacggg gttccatcct tttggatgag
     1621 cactccgctg acggtaaatg ccggctgcat gctgattctc acgacgaagc tttctctccc
     1681 ccgcggcgaa agcgaataca cgaaagagct tccggtggag aattcggtgg gaccgctgga
     1741 gaaaacggcg acggtgttgg tcgtattagt ggaaagccca ttgtacaatg cgcttccgaa
     1801 gggacgcctc ggcacggcca tagggtacct gaacaacca
//
LOCUS       DAOPUZ010000278         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_69991_length_1834_cov_3.272063, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000278 DAOPUZ010000000
VERSION     DAOPUZ010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69991_length_1834_cov_3.272063"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="PKN79_05080"
     CDS             <1..1081
                     /locus_tag="PKN79_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HNZ95170.1"
                     /translation="SSPKLIKQNGGKHTATKSLAESLGLTVSGRRFVIWDSFTDNLTY
                     MSPSLKLFDDGLWVHLWGFETKVFLNIREVEDSDGVYAELYERIGGQGIANFEEEIMA
                     LRLRPVTEAMENLRSESFFSLLSSIFDRTSSSKAERTLLLSLGEAYAHLTTAYELLHP
                     QTKKILNHPPRDPDVKAIMETVKRLNTLFADHSTRLFSQSRLLLDELEVIVSSAFFLN
                     PFVGDDAGIEEVITLAERLQLCRFYAEKLNKAGFVGDDQRKACQSGAIVVCAHRAYRK
                     GEKPQETLARLLEEERVRTYGLVNEYQGVIWFDKERMQELIFLSALSIAMNEPEFDPT
                     SYVKTLFDAQLNASYRLKRLLALPE"
     gene            1087..>1834
                     /locus_tag="PKN79_05085"
     CDS             1087..>1834
                     /locus_tag="PKN79_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNZ95171.1"
                     /translation="MEPYHIDRTALEHFCTHVFIALGLSEEEAVDSSEILVSADARGI
                     ASHGVARLWRYENGLRLGIMSGGIQPTILRETPISLVLDANGAMGMSLSRKTMDQVIE
                     KAKVSGVGFASIRNSNHFGIAGYYSEMAARSDMIGIAMTNTAALGVPTFGTTAMFGTN
                     PIAVAIPGHEGKLFSLDMSTTGVTRGKIEVYNREGHPLPSGWAVDPTGAVTSDAARLL
                     DDMLYQRGGGLLPLGGAGEELGGHKGYGLAV"
BASE COUNT          405 a          547 c          508 g          374 t
ORIGIN      
        1 ctcctccccc aagctgataa aacaaaacgg gggcaagcac accgccacca agtcgctggc
       61 ggaaagtctc gggttgacgg taagcggccg tcgcttcgtc atctgggatt ccttcaccga
      121 caatcttacc tatatgagtc cttcactgaa gctctttgat gacggactct gggtccacct
      181 ctgggggttt gaaacgaagg tgttcctcaa tatccgtgaa gtcgaagact ccgatggcgt
      241 ctatgccgag ctgtacgaac gaatcggcgg tcagggaatc gccaactttg aagaggagat
      301 catggctctc cgcctccgcc cggtcaccga ggcgatggag aacctccgct cggaaagctt
      361 cttctccctc ctttcgtcga tctttgaccg gacaagctcc tccaaagcgg aacgaacgct
      421 tctcctctcc ttgggagaag cgtatgctca cctgacgacc gcctacgagc tgcttcaccc
      481 tcaaacgaaa aagatcctca accatccgcc gcgtgatccc gatgtaaaag cgatcatgga
      541 gaccgtcaaa cgcctcaaca ccctctttgc cgaccactcc acccgtctct tcagccaaag
      601 ccggctgctc cttgacgagc tggaggtgat cgtcagctcg gcgttcttcc tcaatccgtt
      661 cgtcggagat gacgccggga tagaagaggt gatcaccctt gccgaacggc ttcagctctg
      721 ccgcttctac gccgagaagc ttaacaaagc gggatttgtc ggtgatgatc agcgcaaagc
      781 gtgccaaagc ggtgcgatcg tggtgtgcgc ccaccgagcg tacagaaaag gggagaagcc
      841 acaggaaacg cttgcccgac ttttggagga ggaacgggtt cgcacctacg gcttggtgaa
      901 tgagtatcag ggggtcatct ggtttgacaa ggaaaggatg caggagctca tcttcctcag
      961 tgcgctctcc atagcaatga acgagccgga gttcgatccc acctcctacg tgaaaaccct
     1021 tttcgatgct caattaaacg cctcctaccg actcaaacgc ctcctcgccc tcccggagtg
     1081 acaaggatgg aaccgtacca tatcgaccga acggcattgg aacacttttg tactcacgtt
     1141 ttcatcgccc tcggcctttc agaggaggag gcggtcgact cctccgagat tcttgtcagt
     1201 gcggatgccc gcggaatcgc aagccacggt gtcgcccgcc tctggcgcta tgaaaacggc
     1261 cttcggctcg ggatcatgag cggcggaatt caaccgacga ttcttcggga aaccccgatc
     1321 tccctcgtcc ttgatgccaa tggggcgatg ggcatgagcc tcagcaggaa gacgatggat
     1381 caagtgattg aaaaggcgaa ggtttccggt gtcggattcg cctccattcg aaacagcaac
     1441 cacttcggaa tcgccggata ctactcggag atggccgccc gaagcgatat gatcggcatc
     1501 gctatgacaa acaccgccgc ccttggcgtt cccacgttcg gaaccacggc gatgttcggc
     1561 acgaatccga tcgccgtagc gattcccgga catgagggga agttgttcag ccttgacatg
     1621 tccacgacgg gagtgactcg gggaaagatc gaagtgtaca accgggaagg acatcccctt
     1681 ccttcaggat gggcggtcga tccgaccgga gcggtaacct ccgatgccgc ccggcttctt
     1741 gatgacatgc tctatcaacg gggcggcggt ctccttccgc ttggcggagc cggcgaggag
     1801 ctcgggggac acaagggtta tggcctggcc gtcc
//
LOCUS       DAOPUZ010000279         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_70360_length_1828_cov_2.874788, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000279 DAOPUZ010000000
VERSION     DAOPUZ010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70360_length_1828_cov_2.874788"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="PKN79_05090"
     CDS             <1..1474
                     /locus_tag="PKN79_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNZ95172.1"
                     /translation="YYRNTREEEFIANSTRHPMRITMKLGAKKDGTLTGVYMDVRANT
                     GPYGNHCLTVPMNACSKTLPLLKCDNMKFDVYTYYTNIPPTGAYQGYGAPKGTYAMMT
                     ALAELADGLGIDYYDLVDKNKIESGYMLEILRGLGEGREGKVVPVGSCGLGEALAKGA
                     RMIDWGKKEKGPGGDWKIGKGFAMIQQGSGLPGLDHSNAWAKLLPDGNIQLFSGGADI
                     GTGLDTIHAKIAAEGLFVTLDQINVTSGDTDSCPFDTGAYASSGTYFSGNATYYAIED
                     LKRNILDEAAHQLGEHVEDLALRAPGEVYSTKTGASVSYATLGYNALQGDGRGQVMGK
                     GSFVTEHNSIPYGAHFVQVAVNTRTGQIKVEKYYALQDAGTPINPEMALCQMYGAALK
                     SIGHTLYEDMTIDEKGYIQNVRLGDYGVPMITEQPEDFKAILIDVDDEVGPYGAKSIS
                     EISTNGAAPAIAVAIHDAVGVWMRSWPFTPEKLLREMGKL"
     gene            1584..>1828
                     /locus_tag="PKN79_05095"
     CDS             1584..>1828
                     /locus_tag="PKN79_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HNZ95173.1"
                     /translation="MKDKTAIKTMIEKLKTLKSEGMYLDDFFLTWDKTDDELAAVFQV
                     AEILRAMREANISPKVFSSGLGISNFRDNSTRTRFSFA"
BASE COUNT          439 a          499 c          530 g          360 t
ORIGIN      
        1 ctactatcgg aatacccggg aagaggagtt catcgccaac tccacccggc acccgatgcg
       61 gatcacgatg aagctcggag cgaagaagga cggaacgctc accggagtat acatggatgt
      121 ccgggcgaac accggtccgt atggaaacca ctgtctgacc gttccgatga acgcctgttc
      181 gaagacgctg ccgctcctta aatgcgacaa catgaaattc gacgtctaca cctattacac
      241 caacatcccg ccgaccggag cgtatcaggg ctacggcgcc cccaaaggca cctatgccat
      301 gatgaccgcc cttgcggagc tggcggatgg gcttgggatc gattactacg acctggtgga
      361 caagaacaag atcgagtcgg gctatatgct tgaaatcctc cgcggcctcg gtgaaggccg
      421 agagggcaag gtcgtcccgg tcggctcctg cggtcttggg gaagcgcttg ccaagggcgc
      481 gaggatgatc gactggggca agaaggaaaa gggccccggc ggagattgga agatcggcaa
      541 gggctttgcg atgatccagc agggcagcgg cctgcccggt ctcgaccaca gcaatgcctg
      601 ggcgaaactg cttcccgacg ggaacatcca gctcttttcc ggcggagcgg atatcggaac
      661 cggtttggat acgattcacg ccaagatcgc tgccgaaggc ctcttcgtca cccttgacca
      721 aatcaacgtt accagcggtg atacggacag ctgtcccttc gataccggcg cgtatgcctc
      781 ttcggggacg tacttcagcg gaaatgccac ctactatgcg atcgaagatc tgaagcggaa
      841 catcctcgat gaagccgccc accagctcgg cgagcacgtt gaggatctcg cccttcgtgc
      901 acccggggag gtgtactcga ccaaaacggg agcatcggtc tcctacgcca cgctggggta
      961 taatgcgttg cagggcgacg gtcgcggtca ggtgatgggc aagggttcct tcgttacgga
     1021 gcacaactcg attccgtacg gagctcattt tgtccaggtc gcggtcaaca cccgaaccgg
     1081 gcagatcaag gtcgagaagt actacgccct tcaggacgcg ggaaccccga tcaatccgga
     1141 gatggcactc tgccagatgt acggagcggc gttgaagtcg atcggccata ccctctacga
     1201 ggatatgacg atcgatgaga aggggtatat ccagaacgtc cgcctgggcg actatggcgt
     1261 gccgatgatc accgagcagc cggaggattt caaggcgatc ctgatcgacg tcgatgacga
     1321 ggtcggtccc tatggagcga aatcgatcag tgagatttcg accaacgggg cagccccggc
     1381 gattgcggtt gcaattcatg atgcagtagg agtttggatg aggtcatggc ccttcactcc
     1441 cgagaagctg ctccgggaga tgggcaagct ctgatcccct tgaatgcaac aaactgcaac
     1501 aaatttctgg acaataatat tctgctgtgg tagagtatta ttgtccggat ttttttcgga
     1561 acattaccac ggaggctgca tgaatgaaag acaaaaccgc cattaaaaca atgatcgaga
     1621 agctcaaaac gctgaagagc gaagggatgt atctcgacga cttcttcctt acctgggaca
     1681 aaaccgatga tgaacttgcc gcagtgtttc aagtcgccga aattcttcgc gcgatgcggg
     1741 aagcgaacat ctcgccaaaa gtgttttcaa gcggtcttgg aatttccaac ttccgcgata
     1801 attcaacgcg aacccggttc agctttgc
//
LOCUS       DAOPUZ010000280         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_70595_length_1824_cov_3.858112, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000280 DAOPUZ010000000
VERSION     DAOPUZ010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70595_length_1824_cov_3.858112"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PKN79_05100"
     CDS             complement(<1..1040)
                     /locus_tag="PKN79_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ95174.1"
                     /translation="MKKVLGITLVLVTCVLLGVSAQGAKEQVVKPLEFSVFYSDNATL
                     PFKDTWLTVTEIQKRTGVKVNWEIIPIADYKTKVSLALNTGTNAPDVILYQSTKGENA
                     ALALNGAIVPISDYPEWTPHFNARVKEFGLENDVDLLKLKDGKRYYMPALFDVPFYDG
                     GLILREDLLKKYNLPVPKTFDDLYRVLKEFKRHAPDSYPLTILAGPRVLYRMTMPSWG
                     VSLGKNGASGTNTLSWDYDKKQYFAGAISEEYKSYITFFRKLYAEGLLDPEMAEPIDG
                     DRWTQKLATGKAMASYAYYDQIGGVEANSTIPGFKLQMYPALAGPAGAHHQEKSRTGG
                     GVMFPIKTSRRA"
     gene            complement(1118..1612)
                     /locus_tag="PKN79_05105"
     CDS             complement(1118..1612)
                     /locus_tag="PKN79_05105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ95175.1"
                     /translation="MPDMLVKLYELPGSSELLKDLAKEKIRIHRALAPDKERVLNWVG
                     EHSTAGARSEADVCFANQPISLFLATRKREILGFACYNTTAKNFFGPTHVLKSMRGKG
                     IGKALLIASLEALKAEGYAYAIIGGVGPAEYYEKTVGATIIEGSTPGIYRDYLGSGLV
                     FDDE"
     gene            complement(1605..>1824)
                     /locus_tag="PKN79_05110"
     CDS             complement(1605..>1824)
                     /locus_tag="PKN79_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNZ95176.1"
                     /translation="QLALANALKEVTQVCVFALRNPYDLSSLNPSIRSYAAWSYTPMT
                     FEAIARVLKGEIQATGLLPVHLGGRTDA"
BASE COUNT          405 a          524 c          456 g          439 t
ORIGIN      
        1 tcggcacgac ggctggtttt gatcgggaac atgacaccgc caccggttcg gctcttctcc
       61 tggtggtgag ctccggccgg accggcaaga gccggataca tctgcagctt gaagcccgga
      121 atggtgctgt tcgcttcgac tccgccgatc tggtcgtagt acgcatagga agccatcgcc
      181 ttgccggttg caagcttctg ggtccagcgg tcgccgtcga tcggctcggc catctcggga
      241 tccagcagac cttcggcata gagcttgcgg aagaacgtga tgtaactctt gtactcttcg
      301 ctgatcgctc cggcaaagta ctgcttcttg tcgtagtccc agctcagggt gttcgtgccg
      361 ctcgcgccgt tcttaccgag ggagactccc catgagggca tcgtcatgcg atagagaacc
      421 cgcgggcctg ccagaatcgt gagcggatag gagtcgggag catggcgctt gaactctttc
      481 agcacgcgat acagatcgtc aaacgtcttg ggtacgggaa gattgtactt tttcagcaga
      541 tcttcgcgga gaatcaatcc accgtcgtag aacggtacat caaagagagc cggcatgtaa
      601 taccgtttgc cgtctttgag tttcaacaga tcgacatcgt tctccagacc gaactccttg
      661 actcgagcgt tgaagtgagg ggtccactcc ggataatcgc tgatcggcac gatcgctccg
      721 ttgagcgcaa gagccgcatt ttcacccttc gttgactgat acaagatcac atccggagcg
      781 ttggttccgg tgttcaacgc aagggagact ttcgttttgt aatccgcgat cgggatgatc
      841 tcccaattca ccttcactcc tgtccgcttc tgaatctcgg tgaccgtgag ccacgtatcc
      901 ttgaacggaa gcgttgcatt atcggagtag aatacggaaa actccaaggg tttaaccacc
      961 tgttccttcg ccccttgagc gcttacgcca agaaggacac atgtgaccaa cacgagtgtg
     1021 atacctagga cttttttcat agataccctc ctttaagtta tatgtgaaaa acctcaaccg
     1081 gtttctcatt cactgctatg ggatgcattt catcccccta ttcatcatca aaaaccaatc
     1141 cgcttccaag gtaatcccga tagatgcccg gagtcgaacc ttcaataatc gtcgctccca
     1201 ccgttttctc atagtactcg gccggaccta ccccgccgat gatcgcatat gcataccctt
     1261 ccgccttgag ggcctccagg ctcgcgatca ggagcgcttt tccaatcccc tttccccgca
     1321 tcgatttcag cacatgcgtc ggaccgaaga agttcttcgc cgtcgtgtta taacaggcaa
     1381 accccagaat ctcccgcttc ctcgttgcaa gaaagagaga aataggctga tttgcaaagc
     1441 acacgtccgc ttcgcttctt gcccctgccg tggagtgttc acccacccag ttcaataccc
     1501 gctccttgtc gggagcaagg gctcgatgga ttcgaatctt ctccttcgca agatccttga
     1561 gcagctcgct tgagccggga agctcataga gtttgaccag catgtcaggc atcggttctt
     1621 cctcccaaat gaaccggcag gagaccggtc gcttgaattt cacctttcaa aacccttgcg
     1681 atcgcttcaa aggtcatcgg cgtatacgac catgcggcgt aggaacgaat cgaaggattc
     1741 aaggaggaaa ggtcgtaggg attccgcaag gcgaatacgc acacctgagt tacctccttt
     1801 aaagcatttg ccaaggcaag ctgt
//
LOCUS       DAOPUZ010000281         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_70919_length_1819_cov_2.078231, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000281 DAOPUZ010000000
VERSION     DAOPUZ010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70919_length_1819_cov_2.078231"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PKN79_05115"
     CDS             complement(<1..526)
                     /locus_tag="PKN79_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ95177.1"
                     /translation="MQLLPDIYQLSGSLAGLGGDQILGPWDECNSYLVDGGSSYILID
                     AGNGENSGLIFKRMEMWGLDPDKISTLLLTHAHWDHAGGARLFKDRGVEILSSKETAD
                     SVASADHRCAGFLYHREMTPVTVDRIVGDREIVTIDALEFEAIHLPGHTLGCMAYLLT
                     FNDRRILFSGDVIGT"
     gene            complement(536..1156)
                     /locus_tag="PKN79_05120"
     CDS             complement(536..1156)
                     /locus_tag="PKN79_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNZ95178.1"
                     /translation="MLDVYERYPQLEGIRKEIEAAADLLVKAAKKGKMILVAGNGGSA
                     ADSEHIVGELMKSFIRQRPIERSVAETLIEVDQEHGSYLAMKLQSPIKAIALPTHASL
                     SSAFANDVDPHLTFAQLTLGWGEEGGVFWGISTSGNAKNVNLAASVAKAKGMKVLAFT
                     GSDGGELAKKADVAIRAGESETYKVQELHLPIYHYLCIRIEEALFS"
     gene            complement(1149..>1819)
                     /locus_tag="PKN79_05125"
     CDS             complement(1149..>1819)
                     /locus_tag="PKN79_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ95179.1"
                     /translation="PLWDDIEIVRYFEERFGIPTFLENDANAWALAEWYWGAGVGSSS
                     MIFLTFGTGLGAGLILDSRLYRGVNGLAGEVGHIRIADDGPMCYYKKGSWESYCSGCG
                     ISGLYKIATGQERSAKEVCALADANDPAAKKVLEESAAKLGLGIALLIDLLNVERIII
                     GSIFERSEHLFRSIMEEVIEKEALQTSRESCTILPSGLGDRLGDMAALGVARDGYQRS
                     TRNA"
BASE COUNT          360 a          573 c          491 g          395 t
ORIGIN      
        1 gggtgccgat cacatcgccc gagaagagaa tcctccggtc attgaacgtg aggaggtacg
       61 ccatgcagcc cagagtgtga ccgggaagat ggatcgcctc gaattccaga gcatcgatcg
      121 ttacgatctc ccggtcgcct acgatccgat cgacggttac cggagtcatc tccctgtggt
      181 acaagaagcc cgcgcaccga tgatcggcgg aggccaccga gtcggcggtc tccttgcttg
      241 acaggatttc cactccccga tctttgaaga ggcgggctcc ccctgcgtga tcccaatggg
      301 cgtgggtgag aagcagggtt gagatcttat cgggatcgag gccccacatc tccatccgct
      361 tgaagatgag gccgctgttc tccccgttcc ccgcatcgat gaggatgtat gaagacccgc
      421 cgtcgacgag gtagctgttg cactcgtccc acggaccgag gatctgatca ccgccgaggc
      481 cggccaatga tccggaaagc tgatagatat ccggcagcag ctgcatgcgc ggctcctagg
      541 aaaaaagggc ctcttcgatc cggatgcaca gatagtggta gatcggcagg tgaagctcct
      601 ggaccttgta cgtttcgctt tctcccgccc tgatcgcaac atccgccttt ttggccagct
      661 cgccgccgtc gctgccggta aaagcaagaa ccttcatccc tttcgccttg gcgacgcttg
      721 cggcaaggtt cacgtttttt gcgttgccgc tggtcgagat gccccagaag accccccctt
      781 cctcgcccca gccgagggta agctgggcga aggtaaggtg gggatcgaca tcattggcaa
      841 acgcgctgct caaggaagcg tgggtcggaa gagcgatcgc cttgatcgga ctctgaagct
      901 tcatcgccag atacgagcca tgctcctggt ccacctcgat gagcgtttcc gccacgcttc
      961 gctcgatcgg ccgctgtcgg atgaacgatt tcatcagctc cccgacgata tgctcgctgt
     1021 ccgccgccga tccgccgttt ccggctacaa gaatcatctt gcctttcttt gcggctttga
     1081 ccaaaaggtc cgccgcagct tcaatctctt tcctgattcc ctcaagctga ggatatcgtt
     1141 catacacgtc aagcattgcg tgtgctcctt tggtacccgt cccgggcgac tccgagcgcc
     1201 gccatgtctc caagccgatc accgagaccg gagggaagaa tcgtgcagga ttcccggctt
     1261 gtctgaagag cttctttttc gatgacctct tccatgatcg accggaacag gtgttcgctg
     1321 cgctcgaaaa tgcttccgat gatgatccgt tcgacattca gcaaatcgat caggagcgcg
     1381 atccccaatc cgagcttggc ggcgctttct tcgagcacct tctttgccgc cgggtcgttc
     1441 gcatcggcaa gggcgcatac ctcttttgcc gaacgttcct gtcctgtagc gatcttgtat
     1501 aaaccgctga tgccgcatcc gctgcaatag ctctcccacg agcctttttt ataataacac
     1561 atcggtccgt catcggcgat gcggatgtgc cccacctcgc cggcgaggcc attgaccccc
     1621 ctatagagcc gggaatccaa gatcagcccg gccccgaggc cggtgccgaa ggtcaggaaa
     1681 atcatcgacg agctgccgac ccccgccccc cagtaccatt cggcaagagc ccaggcgttt
     1741 gcgtcatttt ccagaaacgt gggaatcccg aaccgctctt cgaaatacct gacgatttcg
     1801 atatcatccc acagcggca
//
LOCUS       DAOPUZ010000282         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_70948_length_1818_cov_3.874645, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000282 DAOPUZ010000000
VERSION     DAOPUZ010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70948_length_1818_cov_3.874645"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /gene="pheS"
                     /locus_tag="PKN79_05130"
     CDS             complement(<1..270)
                     /gene="pheS"
                     /locus_tag="PKN79_05130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606342.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 2 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNZ95180.1"
                     /translation="MMDIDVKNLHPLEIRLLRHVSLGEAIDAKRLIGELGYNVGQCNQ
                     SFSWLEAKGFIKEEARIRRTLYELTDVGRFQAQEGTPAERIFQFLK"
     gene            complement(341..>1818)
                     /locus_tag="PKN79_05135"
     CDS             complement(341..>1818)
                     /locus_tag="PKN79_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNZ95181.1"
                     /translation="CENNGYGMSASRDRMVSAPSIAKRAEGYNMPHNTVDGNDVEAVY
                     AAAREAVRSIRSTGRPYLLEVLTYRLCGHSKSDDCHYRTKEEEAEWEAKDPITRFCRT
                     LIEDELFSEDEVSEIILSGRKQVEEAAMRARAVKDEHLTFEEAEALVYAPEDPQVYGV
                     VNQTSRLTYREAIRFALAEEMARDKAVHLIGEDIGLYGGCFKVTGDLHANFQDRVHET
                     PVSEEAITGMGVGASFLGIRPVVEIMYGDFATLASDPIINHAAKAHFISAGQLSAPVV
                     VRMPVGSGTGHGAQHTQSLESMFTNVPGLIVVAPSCPGDAKALLKSAIRSNNPVLFFE
                     HKGLYDTIGPVGDKEYLMPLGKAIVKKRGSDVTIVAYSKAVTTALSAASILSAQDEIE
                     VEIIDLATLKPLDAETILRSVRKTGRLLVVHDSPEFAGFGAEILAAVAGDESAFRSLK
                     TVPKRLCGKELPIPFAQELEKNAVVSKEEIVKSVRLLFTAG"
BASE COUNT          353 a          525 c          534 g          406 t
ORIGIN      
        1 cttgaggaac tgaaagattc gctcggccgg ggtgccctct tgggcttgga acctgcccac
       61 atcggtcagt tcatacaagg tccgccgtat ccgcgcttct tcttttataa agcctttcgc
      121 ttcaagccag ctgaagctct ggttgcattg gccgacattg tacccgagct cgccgatcag
      181 gcgtttggcg tcgatcgcct ctcccaagga cacatgcctc aggagacgga tctccaaggg
      241 atggaggttc ttcacatcga tatccatcat caatactcct cttcggaccg gatggcccaa
      301 gtggtttgta ttctatcgat tgagcgggaa aaaggaaagg ctatccggcg gtgaagagca
      361 agcggacgct ctttacgatc tcttccttcg acaccactgc atttttttcc agttcctggg
      421 cgaagggaat cggaagctct ttgccgcaga ggcgcttcgg cacggtcttc aaggaacgga
      481 aagccgactc gtcgccggcg accgcagcga ggatctccgc accgaagccg gcgaactcgg
      541 gtgaatcatg aacgacaagc aatcggccgg tctttctgac cgacctgagg atcgtctcgg
      601 catcgagggg cttgagcgtg gcaaggtcga tgatctcgac ctcgatctcg tcctgagcgg
      661 aaaggatcga agcggcggac aaggccgtcg tcaccgcttt ggagtatgca acgatcgtca
      721 cgtcgcttcc ccgcttcttt acgatcgctt tgcccagcgg catgagatac tccttgtccc
      781 cgaccggccc gatagtgtcg tacaagccct tgtgttcgaa aaacagcacg gggttgttgg
      841 agcggatcgc actttttaaa agcgctttgg catcccccgg gcacgacgga gcaacgacga
      901 tcaggcccgg aacgttggtg aacatcgatt cgagggattg ggtgtgctga gctccgtgac
      961 cggtgccgct gccgacgggc atccggacga cgaccggggc ggagagctgg ccggcgctga
     1021 tgaaatgggc tttcgccgcg tggttgatga tcgggtcgct ggcaagggtg gcgaagtctc
     1081 catacatgat ctcgacgacc ggtcggatgc ccagaaacga ggcgccgacc cccatcccgg
     1141 tgatcgcttc ttcgctgacg ggggtttcat ggacccggtc ctgaaagttc gcatgcagat
     1201 cgccggtcac tttgaagcat ccgccgtaga gaccgatatc ctccccgatg aggtgcaccg
     1261 ccttgtcacg ggccatctcc tcggccaatg caaagcggat cgcttcccga taggtcaagc
     1321 ggctcgtctg attgacgacg ccatagactt gaggatcttc cggagcgtag accaacgcct
     1381 cggcttcttc aaaggtgagg tgttcgtcct tgacggctcg agccctcatg gcggcttctt
     1441 cgacctgttt acggccggag agaatgattt cggaaacctc atcttcgctg aaaagttcat
     1501 cttcgatgag ggtccgacaa aaacgggtga tggggtcttt cgcctcccat tccgcttcct
     1561 cttcttttgt tcgatagtga cagtcatcgc ttttggagtg tccgcacagg cggtaggtaa
     1621 gcacttccag aagatacggc ctgccggtgg aacggataga tcggaccgcc tcccgagctg
     1681 ccgcgtacac cgcttcgaca tcattgccgt cgacggtgtt gtggggcatg ttgtatcctt
     1741 ccgcccgctt ggcgatcgag ggggcgctga ccatccgatc acgggaagcg ctcatcccgt
     1801 agccgttgtt ctcgcaga
//
LOCUS       DAOPUZ010000283         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_70959_length_1818_cov_3.120250, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000283 DAOPUZ010000000
VERSION     DAOPUZ010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70959_length_1818_cov_3.120250"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PKN79_05140"
     CDS             <1..553
                     /locus_tag="PKN79_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNZ95182.1"
                     /translation="VVIICYSGQTAGQALGALKLAGFNAKSLLSGMGAWKDEPTVTGM
                     NALSARNNVSSPKGEEQEILWEAAKTYFASVGKDGNKLIKSQDLYDALQANPKAFKVI
                     DIRGEKDFNEGHVIGSSHTAWAQFGSILPDLNKKDRIVIVCFSGQTAGQTVGVLRTMG
                     FDAYSLLGGINNGWKPAGLPLEK"
     gene            588..675
                     /locus_tag="PKN79_05145"
     tRNA            588..675
                     /locus_tag="PKN79_05145"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:621..623,aa:Sec,seq:tca)
     gene            677..>1818
                     /gene="selA"
                     /locus_tag="PKN79_05150"
     CDS             677..>1818
                     /gene="selA"
                     /locus_tag="PKN79_05150"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNZ95183.1"
                     /translation="MYETLPQVEALLQHPSIEKWFVHVSRPVAADVLRITIEEFRKRI
                     GTEQKGFDLDEIISACEKALATISCQRLQPVINATGVIIHTNLGRAPISRENWERTGL
                     LNYSYTNLEFDVAKGKRGKRSSFTSLLLTKMSGAEDALVVNNNAAAIFLILSAFAKRK
                     EVIVSRSELVQIGGGFRIPDILKASGAKLVEVGTTNITTLSDYLDAINEKTAMVLRVH
                     RSNFALRGFTEEVGVRDLKNHLPSDLLLVVDQGSGVFDPRIPSETTVGRHIASGADLV
                     SFSADKALGSVQAGCVVGKRELVAKLGKHPLFRTMRIGKTVSSLLEATLIDLLNGVDQ
                     PVGTLLFRSIAEMRKTGSSILERIKSERLSPEGHRVLNEGEALALDP"
BASE COUNT          397 a          562 c          508 g          351 t
ORIGIN      
        1 ggtcgtcatc atctgctact ccggccagac cgccgggcag gccctcggag cattgaagct
       61 cgccggcttc aacgcgaaat ccctcttaag cggcatgggt gcctggaagg acgaacccac
      121 ggtaaccggt atgaacgccc tctctgcacg caacaacgtc tcctccccca agggtgaaga
      181 gcaggagatc ctctgggaag ccgcgaagac ctacttcgct tccgtcggca aggatggaaa
      241 caagctcatc aaatcccagg atctctatga tgcccttcag gcaaacccga aagccttcaa
      301 ggtgatcgac atccgcggcg agaaggactt caacgaagga cacgtcatcg gctcgagcca
      361 caccgcctgg gcgcagttcg gatcgattct tcccgatctc aacaagaagg accgcatcgt
      421 catcgtgtgc ttcagcggcc agaccgccgg ccagaccgtc ggagtcctcc gcacgatggg
      481 cttcgatgca tactctctcc ttggcggcat caacaacggg tggaaacccg ccggtctccc
      541 gctggagaaa taatcgcatc cacaaaaggg gccgcttggc cccttttgga agcagatggg
      601 tcctggggat cccgcggtct tcaaaaccgt tggctgtagt aatcacagcg gcaggttcga
      661 ttcctgcctc ttccgtatgt atgaaaccct ccctcaagtg gaagccctcc tgcagcaccc
      721 ttcgatcgag aagtggttcg tccacgtcag ccgccccgtg gcggccgatg tgcttcggat
      781 cacgatcgaa gagtttcgaa aacggatcgg aactgaacag aaaggattcg atcttgatga
      841 gattataagc gcttgtgaaa aggcccttgc gacgatctcc tgccagcggc tgcaaccggt
      901 catcaacgcg accggcgtga tcatccacac caaccttgga cgggccccga tcagcaggga
      961 gaactgggaa cgcaccggtc ttttgaacta ctcctacacc aacctggagt tcgacgtggc
     1021 caagggcaaa cggggaaagc gttcttcgtt cacctctctt ttgttgacga aaatgagcgg
     1081 tgccgaagac gccctcgtgg tgaacaacaa cgccgcggcg atcttcctga tcctctccgc
     1141 cttcgccaaa cggaaggaag tgatcgtcag ccgctcggag ctggtgcaga tcggcggggg
     1201 cttcaggatt cccgatatcc tcaaagcctc gggagcgaag ctcgtggaag tgggtacaac
     1261 aaatataaca accctctccg attaccttga tgcgatcaat gaaaaaaccg cgatggtcct
     1321 ccgggtccat cgctcgaact tcgcccttag ggggttcacc gaggaagtcg gcgtccgcga
     1381 cctgaaaaac catcttccct ccgatctgct gctggtcgtc gaccagggct ccggggtgtt
     1441 cgatccgcgg attccttccg agaccaccgt cggccggcac atcgcctcgg gtgccgacct
     1501 cgtctccttc tccgccgaca aggctctcgg ctcggtgcag gccgggtgcg tcgtggggaa
     1561 acgggaactg gtcgccaagc ttggaaagca tcccttgttc aggacgatgc ggatcggaaa
     1621 gaccgtctcc tccctcctgg aagccaccct gatcgatctg ctcaacggag tcgaccagcc
     1681 ggtgggcacg ctccttttca gaagcatcgc tgaaatgcga aagacgggaa gttcgatcct
     1741 ggaaaggatc aagagcgagc gcctctcccc cgagggtcat cgtgtcctca atgagggaga
     1801 ggcgctcgct cttgatcc
//
LOCUS       DAOPUZ010000284         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_71007_length_1817_cov_3.422815, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000284 DAOPUZ010000000
VERSION     DAOPUZ010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71007_length_1817_cov_3.422815"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PKN79_05155"
     CDS             <1..992
                     /locus_tag="PKN79_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95184.1"
                     /translation="MMAQSSYESAKLNFANTLYSQIGALLNNEKSIKSTTQKLEDAKK
                     ELSDKLVLGQVKKGSLVYQAQDQAIRLSEGTLKSLEASRDLLIRQFETIMGVPYPDAI
                     TDIREPNLSLAMNAEGSTSVASKKIDVQKAKETLALRIAQYTNRTLVAGGNISYANSQ
                     INTNLGPMTQNSLTLGGSAALAGKSFTVQGAVGGTYDFDKGTLTPVVAISGTWSNDPA
                     FAKDALEIRQLENEVLLAEIAYNNAVDEYIQSSIEVQNGIASWQLEYALLLQTIRYNK
                     DLLKQQRELFENGLVTKKQVEDAAFTVELDEYDLKAMLLKGLQLENEIKRLMM"
     gene            1004..>1817
                     /locus_tag="PKN79_05160"
     CDS             1004..>1817
                     /locus_tag="PKN79_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ95185.1"
                     /translation="MFEMQRSDTATETLVKNQFKKKQRRRRARRFFTWIFILALIAGG
                     LYLYSFYKENNRFPWAEERAPISFVRETDSEVYESIFTQTIDLSSYVEPNDIQQVILR
                     ATGTVTDVYVKEGDSVKKGDVLIALDDINERFDVAQSQDALQAAILTGASDREIELAE
                     LRLTNARNKLDYTKAYANFDGVVASVKVSEGDYFTAGQTAMTIVDRSTLKATVEIDEI
                     DMQYVELGQKAVLYFDSYTDGSIEGYVSYIPMLGRYTNQGIGVMDVELTIEHP"
BASE COUNT          467 a          497 c          512 g          341 t
ORIGIN      
        1 agatgatggc ccagtcgagc tatgaatctg cgaagctgaa cttcgccaac accctctaca
       61 gccagatcgg cgcacttttg aacaatgaaa agtcgatcaa gagcaccact cagaagcttg
      121 aggatgcaaa aaaagaactc agtgacaaac tggttctggg gcaggttaaa aagggcagtc
      181 tcgtctatca ggctcaggat caggcgatca ggctgagcga aggcaccctc aagagccttg
      241 aggcgtcccg ggatctgctg attcgccagt ttgaaaccat catgggtgtc ccgtatcccg
      301 atgcgatcac cgacatccgg gaacccaatc tttcccttgc gatgaacgcg gaaggctcga
      361 cctccgtcgc ttcgaagaag atcgacgttc aaaaggcgaa ggagaccctc gccctccgga
      421 tcgcccaata caccaacagg acgctggtgg caggcgggaa catctcctac gccaacagcc
      481 agatcaatac caaccttggt ccgatgacac aaaacagtct caccttggga ggttcggccg
      541 cgctggccgg caagagcttc accgtccaag gggcggttgg cggaacctac gatttcgaca
      601 aggggaccct gacgcccgtg gtcgccatct ccggaacctg gtccaacgac cccgccttcg
      661 ccaaggatgc gctggagatc cggcagcttg agaacgaggt gctgctggcc gagatcgcat
      721 ataataatgc cgtggatgag tacatccaaa gctcgatcga ggtgcagaac ggcatcgcct
      781 cctggcagtt ggagtatgcg ctcctcttgc agacgatccg gtacaacaag gatctgctga
      841 agcagcagcg ggaactcttt gagaacggcc ttgtgacgaa gaaacaggtg gaagacgccg
      901 cgtttaccgt tgaacttgat gaatacgacc tgaaggcgat gctgctgaaa ggcttgcagc
      961 ttgaaaatga aatcaagcgc ttgatgatgt aaggagaaag agcatgtttg agatgcagag
     1021 aagcgatact gcaacggaaa ccctcgtgaa gaaccaattc aagaagaaac aaaggaggcg
     1081 gagagcccgc cgcttcttca cctggatctt cattctcgcc ctgatcgccg gcgggctcta
     1141 cctctacagt ttttacaagg agaacaaccg cttcccctgg gcggaagagc gggctccgat
     1201 cagcttcgtc cgggaaacgg actccgaggt ctatgaatcg atcttcaccc agacgatcga
     1261 tctctcctcc tacgtcgaac ccaacgatat ccagcaggtg attctccggg cgaccggaac
     1321 cgttacggac gtatacgtga aggaagggga cagcgtgaag aagggcgatg tgctgatcgc
     1381 cctagatgac atcaatgagc gcttcgatgt ggcgcaaagc caggatgccc tgcaggcggc
     1441 gatcctcacc ggtgcaagtg accgggagat cgagttggcg gaacttcggt tgaccaacgc
     1501 caggaacaag ctggattaca ccaaagccta cgccaacttc gacggcgtcg tcgcttcggt
     1561 gaaggtctcc gaaggggact acttcaccgc cggacagacg gcgatgacga tcgtcgaccg
     1621 gtcgacgctg aaggcgaccg tcgaaatcga cgaaatcgat atgcaatatg tcgaactcgg
     1681 ccagaaagcc gttctttact tcgattccta caccgacggc tcgattgaag gatatgtcag
     1741 ctacattccg atgctcggcc ggtataccaa tcaggggatc ggggtgatgg acgtcgaatt
     1801 aacgattgaa catccgc
//
LOCUS       DAOPUZ010000285         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_71016_length_1817_cov_2.829171, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000285 DAOPUZ010000000
VERSION     DAOPUZ010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71016_length_1817_cov_2.829171"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="PKN79_05165"
     CDS             <1..1697
                     /locus_tag="PKN79_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNZ95186.1"
                     /translation="AVLDELTQTLLALKERQKVANQALLGVEEMRRYLAESKWQHLDL
                     RVGSVKEGKSEQLEEKLSPYGAFVEPIDEEFVTVLSLRRDLAQIDPLLDKFGWTEESD
                     PLLQKQAVVLVRTRLETEHQKLIDKRGEIEQSVKRAIAERKEELHAIWSNLRLNELSD
                     QIKSYFSYTRNTTLFSGWVPADQAESVRNAIVEASGGQCVVEWTEATEVPRESVPVAV
                     TSPKVLEPFQNMVNNYATPEYGTVNPTIFVMIAYLSMFGLMFGDVGQGLVLLLVGLFG
                     SRSYRRNPEKKEGLLTRNVTNLLVYLGLSSMLFGALFGSYFGLPLLPPLWFNFEAAVE
                     GHAPATALVRDVYGILGITIRFGIIIIYTGLVLNWVNLFRKKSFVPMLLDKNGVVGGV
                     LFGTGIYMAFGFVGSSYRSFPSDPWIIPTLVICLILLFAKPYVQHFVDLKQGRTDKTF
                     GQVAAGSIMEFLVDVLEIFTGYLSNTLSFMRVAGLGIAHASLMGTFKYLQSMVGGVAG
                     VLIFIAGNLLVIVLEGLSAGIQSLRLNYYEFFSRYFTGRGIAYRPVGLKRSSSDNR"
BASE COUNT          378 a          501 c          521 g          417 t
ORIGIN      
        1 gggcggtcct cgatgaactg actcaaaccc tgcttgcgct taaagagcgc caaaaagtcg
       61 ccaaccaagc cctgttgggg gtggaggaga tgcgccgata cctcgctgaa tccaagtggc
      121 aacatctgga tctgcgagtc ggaagcgtga aggagggaaa gagcgaacag ctggaggaga
      181 agctctcacc gtacggcgca ttcgtcgagc cgatcgacga ggagtttgta accgtgcttt
      241 cccttcggcg ggatctcgcg caaatcgatc cgcttttgga taagttcggc tggacggagg
      301 aaagcgatcc gctgcttcag aagcaggcgg tcgtgcttgt ccgaacccgt cttgaaacgg
      361 agcaccaaaa gctcattgat aaacggggtg agattgagca atcggtcaaa cgagcgatcg
      421 ccgaacgcaa agaagagctc catgcgatct ggtcgaatct ccggctcaac gagctcagcg
      481 accagattaa gagctatttc tcctataccc gcaatacgac cctcttcagc ggatgggttc
      541 ccgccgatca agctgagagc gttcgaaacg caatcgtcga agcaagcggc gggcagtgcg
      601 tcgtggagtg gacggaggcg acggaggttc cccgtgaaag cgttccggtc gcggtaacca
      661 gccccaaagt gctggaaccg ttccagaaca tggtcaacaa ctacgccaca ccggagtacg
      721 gcaccgtcaa cccgacgatc ttcgtgatga tcgcctacct ttcgatgttc ggcctgatgt
      781 tcggcgatgt aggacagggg ctcgtcctgc tgttggtcgg actcttcgga tcgcggagct
      841 accgccgcaa tccggagaag aaggaagggc tcctgacgcg caatgtcacg aacctgctcg
      901 tctacctcgg gctctcctcg atgctcttcg gagcgctgtt cggatcctac ttcggcctgc
      961 cgctcctgcc gccgctgtgg ttcaacttcg aagcggcggt cgaagggcat gctcccgcca
     1021 ctgcactggt ccgtgacgtg tacgggattc tcggcattac gatcaggttc ggaatcatca
     1081 tcatctacac cggcctcgtt ttgaactggg tcaacctgtt caggaagaaa tccttcgttc
     1141 cgatgctgct tgacaagaac ggggtcgtcg gcggtgtcct gttcgggacc ggaatctata
     1201 tggcgttcgg gttcgtcgga tcgagctatc gctccttccc ctccgatccc tggatcatcc
     1261 ccacgctcgt catctgcctg attctgctct tcgccaagcc gtatgtgcaa cacttcgtcg
     1321 accttaagca agggaggacg gacaagacgt tcggccaggt ggccgccggc tcgatcatgg
     1381 agttcctggt ggatgttctg gagattttca ccgggtatct ctccaacacc ctttccttca
     1441 tgcgagttgc cggtcttggc atcgcccacg cttcgttgat gggaacgttc aagtatcttc
     1501 agtcaatggt cggtggagtg gcgggtgttc tcatcttcat cgcgggcaac ctcttggtaa
     1561 tcgtcctcga gggcctcagt gcaggtattc aatcgctcag gttgaactac tacgaattct
     1621 tctcaaggta ttttaccggt cgaggaatcg cttacaggcc agttggactc aagcggtctt
     1681 cgtccgacaa tcgataaatt ttagtgcatt tgataggagg gtcttgtatg accaaatcga
     1741 tgttcagaag gaaagtctcg atgagcttag ttgcaacggc cttggtactg gtaggttcag
     1801 gcttcatctt cgccccg
//
LOCUS       DAOPUZ010000286         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_71071_length_1816_cov_3.616695, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000286 DAOPUZ010000000
VERSION     DAOPUZ010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71071_length_1816_cov_3.616695"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /gene="thrH"
                     /locus_tag="PKN79_05170"
     CDS             complement(<1..112)
                     /gene="thrH"
                     /locus_tag="PKN79_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968032.1"
                     /note="catalyzes the formation of serine from
                     phosphoserine; also has phosphoserine:homoserine
                     phosphotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNZ95187.1"
                     /translation="MKIVCLDLEGVLVPEIWINVAKRTGIEELRRTTRDEP"
     gene            complement(156..887)
                     /gene="cobS"
                     /locus_tag="PKN79_05175"
     CDS             complement(156..887)
                     /gene="cobS"
                     /locus_tag="PKN79_05175"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNZ95188.1"
                     /translation="MSLASALRLLTRFRIPGKEIEQSGRTLFWFPIVGALLGCISCLF
                     ALLPLSAPIRSVLVLIAGVYFTRALHYDGVADLADGLGGGFTKERSLEIMRDSHIGAF
                     GTIALILVVLLQYSLLLDVVEHPPMLILVPALGRLAQVWAASLMNYARQGEGMASSLV
                     RRAKWHHALLPTLQVGLVLLLLFVLHNPYAIPVLAASGGIVLSTTVLCLISRKRLGGI
                     TGDVLGSVEVFGETAALLGIVFALA"
     gene            complement(884..1387)
                     /gene="cobU"
                     /locus_tag="PKN79_05180"
     CDS             complement(884..1387)
                     /gene="cobU"
                     /locus_tag="PKN79_05180"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNZ95189.1"
                     /translation="MIGGGRSGKSTWAEAYARSLGGKRVYLATAEAFDEEMQARIRAH
                     QARRSGQFITIEEPLQLSGRIMNLPSDVRVCLVDCLTVWLGNLMHYEKDLEAYLEELY
                     RVVDDPPCELILVTNETGLGIVPADPMSRIFRDRAGWMNQRLALLADRVVLLMAGIPL
                     AIKGTLP"
     gene            complement(1287..>1816)
                     /gene="cbiR"
                     /locus_tag="PKN79_05185"
     CDS             complement(1287..>1816)
                     /gene="cbiR"
                     /locus_tag="PKN79_05185"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HNZ95190.1"
                     /translation="LIQSEKSVIDLLQRTGVDPLLLGIETLGYPLDLVDSIISRYGLG
                     VTLDIGHLHLMGYDVEDHVKRYASRCRVFHLHGVNEGTDHLGLHRGDREKIDRFLDLL
                     ASLEDGIERVVTLEVFDQESFEGSMEVLHERTREEKPGNLGDRGWTKREKHVGGSIRS
                     IPRREAGLSGDRRSV"
BASE COUNT          441 a          575 c          461 g          339 t
ORIGIN      
        1 ccggctcatc ccgggtcgtc cggcgcagct cttcgattcc cgttcgcttt gccacgttga
       61 tccagatctc cggcaccagc accccttcca aatccaaaca gacaatcttc atatcgtatg
      121 ctccaattgg ctttagtatt gcaaagagtg ttcacctatg caagagcgaa cacgatcccg
      181 aggagggcgg cggtttcacc gaacacctcg acgcttccca gcacatcgcc ggtgattccc
      241 cccaaacgct ttcgggaaat caggcaaagg accgtcgtgc tgaggacgat ccctccagaa
      301 gcggcaagaa ccgggatcgc atagggatta tgtaaaacaa acaggagcag gagaaccaaa
      361 ccgacctgaa gggtgggaag cagggcatga tgccacttcg ccctacgcac caacgaagaa
      421 gccatcccct ccccttggcg ggcgtagttc atcagtgaag ccgcccacac ctgcgccaac
      481 cggcccagag cgggaacaag gatcagcatg gggggatgtt cgaccacgtc gaggagaagc
      541 gaatactgca gcaaaacaac gaggatcagc gcgatcgtgc cgaacgcccc gatatggctg
      601 tcccgcatga tttccaagga tcgttctttt gtaaagccgc cacccagtcc gtcggcaaga
      661 tccgccacgc cgtcatagtg aagggcccgg gtgaaatata ctccggcgat gaggacgaga
      721 acgctgcgaa tcggggcgga aagtggaagc agggcaaaaa ggcaggagat acaacccaga
      781 agagccccca caatgggaaa ccagaagagg gtccgcccgc tctgctcgat ctccttgccg
      841 gggatgcgga atcgggtaag aagacgaagc gcgcttgcca ggctcatggc agtgttcctt
      901 tgatcgccaa ggggatgccc gccatgagga gaaccacccg atccgccaac aaagcaagcc
      961 gttgattcat ccaccccgcc cggtcacgaa aaatccggct catcggatcg gcgggaacga
     1021 tccccaaccc cgtttcattg gtcaccagaa tcagctcgca ggggggatca tccaccaccc
     1081 ggtacagctc ctcaagatat gcttcaagat ccttttcgta atgcatcagg tttcccagcc
     1141 agacggtaag gcaatcgacg aggcacaccc ggacatcgga gggaaggttc atgatccgcc
     1201 cgctcagctg aagaggctct tcgatcgtga tgaactgccc gcttcggcga gcttgatgag
     1261 ccctgattcg cgcctgcatc tcctcatcaa acgcttcggc ggtcgccaga tagacccgct
     1321 tcccgccgag ggatcgagcg tatgcttccg cccacgtgct tttcccgctt cgtccacccc
     1381 cgatcaccaa ggttaccggg tttttcttcc cttgtccgct catggagcac ctccatcgat
     1441 ccttcaaaac tttcctggtc aaacacttca agagtgacca cccgttcgat cccatcttcc
     1501 aacgaagcaa gcagatccaa gaatcgatcg atcttctccc gatcgccccg atgaagaccg
     1561 aggtgatccg tcccctcgtt caccccatga agatgaaaca cccggcaccg gcttgcatag
     1621 cgcttcacat ggtcttccac atcatacccc atcagatgaa ggtgtcctat atccaacgtt
     1681 acacccaacc cataacgact gatgatggaa tcgaccagat caagcggata gcccagcgtc
     1741 tcgatgccca gcaggagggg atcgactccc gttctttgca gcagatcgat gacgcttttc
     1801 tcactctgaa tcagcc
//
LOCUS       DAOPUZ010000287         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_71200_length_1814_cov_3.151222, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000287 DAOPUZ010000000
VERSION     DAOPUZ010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71200_length_1814_cov_3.151222"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /gene="rpsB"
                     /locus_tag="PKN79_05190"
     CDS             complement(<1..500)
                     /gene="rpsB"
                     /locus_tag="PKN79_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002667735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNZ95191.1"
                     /translation="MSVVTMKSLLESGVHFGHQTKRWNPKMARFIFSERNGIHIIDLQ
                     KTSACIIEAYDAVRGVVKQGKSVLFVGTKKQAQQAIETEAKRCGMPYVNHRWLGGMLT
                     NFSTIKKSIATLKKIERMENDGTFNALTMKEISLLEKQKEKLEKNLGGIKDMKDIPDA
                     LFVIDPR"
     gene            complement(570..1142)
                     /locus_tag="PKN79_05195"
     CDS             complement(570..1142)
                     /locus_tag="PKN79_05195"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNZ95192.1"
                     /translation="MSKTIILASKSLGRKLLLEEAGFEVLIQPSKADEEHQRESVEKS
                     VRLLAMRKLEAFLRDRPSPTHPVIASDTLLAFEEIELGQPETREAAFSQLRLLSGATH
                     HVYSAWALWVNGDVHQGCDRCAVTFKELSDDEIEAYLDTGEWVGAAGSYRYQGRGKEL
                     VRSTTGDESTVIGLPMNQIFGILAKALPDW"
     gene            complement(1135..>1814)
                     /locus_tag="PKN79_05200"
     CDS             complement(1135..>1814)
                     /locus_tag="PKN79_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ95193.1"
                     /translation="TADIPRLRTYLGSFLFSGDDVFKRVGILSGGERSRLALLKTLMH
                     PVNLLILDEPTNHLDIAAKKALLQAIREYDGTLIFVSHDAYVIQEAATSILYLTEENP
                     PELFPGDWSYFSYRLEQKEAIPAEAAKEEKKPARSYREERQKHNRLVSLRRQAEESLL
                     TLDVIETELQEVQRLMSQPENYSVEEKILKLVKEEERLRKNLADEEAAWLTLEEERMK
                     LEEELYE"
BASE COUNT          358 a          504 c          461 g          491 t
ORIGIN      
        1 cgcgggtcga tgacgaagag agcatcggga atatccttca tatccttgat gccgccaagg
       61 ttcttctcga gcttctcttt ctgtttctcc aagagcgaga tctctttcat ggtcaacgcg
      121 ttgaaggtac cgtcattttc cattcgctcg atttttttga gtgtggcgat ggattttttg
      181 atcgttgaaa agttcgtgag cattccaccg agccagcgat ggttgacata cggcatgccg
      241 cagcgtttgg cttcggtttc gatcgcctgc tgggcctgtt tctttgttcc gacaaagagg
      301 acgctctttc cttgtttgac gacgcctctg accgcgtcat acgcctcaat gatgcacgct
      361 gaggttttct gtaaatcgat gatgtgaata ccgtttcgtt cgctgaagat gaaacgggcc
      421 attttcgggt tccaccgttt ggtctggtgg ccgaagtgta cgcctgactc aagcaggctt
      481 ttcatggtta ctacggacat aatcctccta ttgacgcacc aaaagcacgt cacccttctc
      541 ctgttacaca ccttgcggtg gagatttccc taccaatccg gcagagcttt agccagtatc
      601 ccaaaaattt gattcattgg caagcctatc accgtgcttt catccccggt ggtcgaccgg
      661 acgagctctt tgccccggcc ttgatagcgg taggaaccgg cggctccgac ccattcaccc
      721 gtatcaaggt aggcttcgat ctcatcatca ctcaattcct tgaatgtcac cgcacatcga
      781 tcacagccct gatgcacatc tccattcacc cacaacgccc acgctgaata tacatgatgg
      841 gtggctccgc ttaagagcct cagctgggaa aaggcggctt ctctggtttc gggctgccca
      901 agctcgatct cctcgaaagc gaggagcgta tcactggcga tgacgggatg ggttggggaa
      961 ggccggtctc gaagaaacgc ctcaagcttg cgcattgcca ataatcggac gcttttctcg
     1021 acgctttctc tttggtgctc ttcatccgct ttgctgggtt ggatcaagac ttcaaacccc
     1081 gcttcttcca gcaagagttt tcttcccaga gattttgatg ccagaataat ggtcttactc
     1141 atacagctct tcctccaatt tcatccgctc ctcttccaac gtgagccacg cggcttcttc
     1201 atcagcgagg tttttccgta atcgttcctc ttctttcacc aatttcagga tcttttcttc
     1261 aacggagtag ttctcaggtt ggctcatcag ccgttgaacc tcctgaagct cggtttcaat
     1321 cacgtcgaga gtgaggagag actcttccgc ctggcggcga agggagacca ggcggttgtg
     1381 cttctgtctc tcttcgcggt aggagcgggc gggctttttt tcctctttcg ccgcttctgc
     1441 gggaatcgcc tccttctgct ccaggcgata gctgaaatag gaccaatccc ccgggaagag
     1501 ctcgggggga ttctcttccg ttaaatagag aatgcttgtc gctgcttcct gaatgacgta
     1561 ggcgtcgtga ctgacgaaga tcagggttcc gtcgtactcc ctgatcgctt gaagaagcgc
     1621 cttcttcgcc gcgatgtcca agtggttcgt cggttcatcc agaatcaaca ggttcaccgg
     1681 gtgcatgagc gtcttcaaaa gggccaagcg ggagcgctcg ccgccgctta agattccgac
     1741 ccgcttgaac acatcgtcgc cgctgaagag gaaggagccg aggtatgttc tcagccgggg
     1801 aatgtcggcg gtcg
//
LOCUS       DAOPUZ010000288         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_71655_length_1807_cov_2.600457, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000288 DAOPUZ010000000
VERSION     DAOPUZ010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71655_length_1807_cov_2.600457"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1095)
                     /locus_tag="PKN79_05205"
     CDS             complement(91..1095)
                     /locus_tag="PKN79_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ95194.1"
                     /translation="MHGCPTLLTGHNADDQLETLLRRLFTAGSLSSLQGIRSRLEQDG
                     ITILRPVLNCPHKDLEAYLVEGGWAWSEDSTNKAETYQRNLLRRRMTGDLLSIFEGAY
                     TALEVQTGRFADLAVFLESSVEKAFEGVDVRGDEASFSLSWFQLLPSTVQELLLFRLA
                     GADSSSAFIANVHRALKADKHAIMESGSHRIVVHRGVVTWKRNEDPWSYAIEAIGDEI
                     VLPGNLVFRREAENRDSTLLRIDGTVLENPVLRLSRDGDEFAGEGGTANVAKILSSQG
                     LPPALRVPVLVDRSGIVAIFARVYGGRDRLAKRFKAPLARRLTNIYSVSRRIDDEIYE
                     "
     gene            complement(1097..1450)
                     /locus_tag="PKN79_05210"
     CDS             complement(1097..1450)
                     /locus_tag="PKN79_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ95195.1"
                     /translation="MNTIERTPPEVIRTLIDKEGRIAVAFSCGADSLALLVGVCEVRD
                     PEDIVALYVNHRLRPEEELEAELVLNRKNAERLGVVFDVLDLGPGAVEDLAKERKKGV
                     EEAARTLRYQLLLRA"
     gene            complement(1401..>1807)
                     /locus_tag="PKN79_05215"
     CDS             complement(1401..>1807)
                     /locus_tag="PKN79_05215"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNZ95196.1"
                     /translation="LIIQPLTYMNRSGSVLNYFRTEKQLIVVCDQLDLPPGMIRIRRK
                     GSSAGQRGLQSIIDAHGSEDFIRIYVGIGRPERGKSVVEYVLAPSDDPLLEVGIQQAA
                     EALVKLLQGFGISEVMDEYNRKNTPRSDQNPH"
BASE COUNT          387 a          556 c          443 g          421 t
ORIGIN      
        1 ggaccgcgat tcagaggatt gttgtcgtcc ttcttcggac cctgccaata ctggttcgga
       61 tcccgctttt caggaccttg atcctgactg tcattcatat atttcatcat ctatccttct
      121 gctcacacta tatatatttg tcaaccggcg ggcaagggga gctttaaagc gtttggccaa
      181 tcgatcccgc cctccgtaga ctctggcgaa aatcgcgaca attccgcttc gatccaccaa
      241 tacgggaact ctcaatgccg gcggcaatcc ctggcttgaa aggattttcg ccacgttcgc
      301 cgttccccct tcccctgcaa actcatcgcc gtcccggctg agccgaagaa ccgggttctc
      361 caataccgtt ccgtcgattc gaagcagcgt tgaatccctg ttttccgctt ccctccgaaa
      421 gaccagattc cccggcagaa cgatctcatc cccgatcgct tcgatcgcat aggaccaagg
      481 atcttcgttc cgtttccacg taacgacccc ccggtgcacc acgatgcggt ggcttccgct
      541 ttccataatg gcgtgcttgt cggccttcag ggccctgtgc acgttcgcaa tgaatgcgga
      601 ggagctgtcc gccccggcca gccggaagag gagaagctct tgaaccgtag aaggcagaag
      661 ctgaaaccag gaaagggaaa acgatgcttc gtctccccga acgtcaacac cttcaaacgc
      721 tttttccacg gatgactcaa gaaacacggc aaggtcggca aaacgcccgg tctgtacttc
      781 caaagcggtg tacgcgcctt caaagattga aagaaggtcg cccgtcatcc gtcttcgaag
      841 aagatttcgt tgataggtct ccgctttgtt ggttgaatct tccgaccacg cccatccacc
      901 ttcgacaaga tatgcttcaa ggtctttgtg aggacaattc aatacaggtc gaagaatcgt
      961 gattccgtct tgttccagcc tgctcctgat cccctgcagg gatgaaaggc tccctgcggt
     1021 gaagaggcgc ctcaacagcg tttccagctg atcatcggcg ttgtggccgg taagcaacgt
     1081 tgggcagccg tgcattttat gcgcgaagca acagctgata gcgcaatgtc cgggcagcct
     1141 cctcgacccc cttctttcgc tcctttgcaa gatcttcgac tgcaccgggt ccgaggtcaa
     1201 ggacgtcaaa aaccacgccg agtcgctctg cgttcttcct gttgagcacc agctccgctt
     1261 ccagctcttc ttcaggacgc aagcggtggt tcacatacag agcgacgata tcttcgggat
     1321 cccgaacctc gcacaccccg accagcagag cgagcgaatc cgcgccgcag ctgaaggcca
     1381 cggcgattct tccctccttg tcaatgaggg ttctgatcac ttcggggggt gttctttcga
     1441 ttgtattcat ccatcacctc cgatatccca aatccctgca gaagcttgac aagcgcctcg
     1501 gccgcttgtt gtattcccac ctccaaaagg gggtcgtcgc tcggtgcaag gacgtattcc
     1561 acgacggatt tcccccgttc aggtcggcca ataccgacat atatgcgaat gaaatcctca
     1621 ctgccatgcg catcaatgat cgattgaagc ccccgctgcc ccgccgacga tccttttcgc
     1681 cggatccgaa tcatccccgg cggcagatcg agctggtcgc agacgacgat cagctgtttt
     1741 tccgtccgga aatagttgag cacggaaccg cttcgattca tataggtaag cggctgaatg
     1801 atcagtg
//
LOCUS       DAOPUZ010000289         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_72063_length_1801_cov_2.439290, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000289 DAOPUZ010000000
VERSION     DAOPUZ010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72063_length_1801_cov_2.439290"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKN79_05220"
     CDS             <1..201
                     /locus_tag="PKN79_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNZ95197.1"
                     /translation="IHRPENYAQLLDRFHFPASVNIEADRYRTTTPFYTVEQLANYEL
                     KEDLGANLEMYNKAWETIRYVD"
     gene            233..1639
                     /gene="purB"
                     /locus_tag="PKN79_05225"
     CDS             233..1639
                     /gene="purB"
                     /locus_tag="PKN79_05225"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNZ95198.1"
                     /translation="MQEFNHQTFISPFTWRYGSEAMRRIFSEVHKRTLLRTIWIALAR
                     AQMQVGLVTEEQLAELEATKDRIDIARATQIESEIHHDLMAEIHTWAEQCPNGGKIIH
                     LGATSMDVLDNMDALRLTEALDLTISKTRELLILFKEKMEAYRELPTMAFTHIQPAEP
                     STVGYRFAQSAQDLKEDLEELIRVRSSIRGKGVKGAVGTAASYAQLLEGTGLTAIKLE
                     EAVMEEIGLAAYTASTQIYTRKQDLRVAHALSGLAATLSKFFFDFRILMSPPIGEWSE
                     YFASKQVGSSAMPFKRNPINSEKINSLCRLVSTHVDLFWQNAATTVLERSLDDSANRR
                     IAIPEIFLATDEILVTAIRTVGRMTVHEEAVGRNLNAYGLFAASERLLMELGRRGADR
                     QAMHELIRNHSLKAWEAVRSGKENPLAKSLSRDKTVLAFVTEEEVLEFLKVEAYIGDA
                     VLRTTMVAKEIDAFLNRD"
BASE COUNT          408 a          578 c          515 g          300 t
ORIGIN      
        1 atccatcgcc cggagaacta cgcccagctt ctggatcggt tccacttccc cgcatcggtc
       61 aacatcgaag cggatcggta tcggaccacc acccccttct acaccgtcga acagctggcg
      121 aactacgagc tgaaggagga tctgggggcg aacctcgaga tgtacaacaa agcctgggag
      181 acgatccgct acgtagattg accccgaaag gcctctcttg tacagtttac ccatgcaaga
      241 gttcaaccat cagacattca tatctccctt cacctggcgc tatggctccg aagccatgcg
      301 ccggattttc agcgaagtcc acaagcggac gctccttcgg acgatctgga tcgccctcgc
      361 ccgggcccag atgcaagtcg gcctggtcac cgaagaacag cttgccgaac tggaagcgac
      421 gaaggatcgg atcgacatcg cccgggcgac acagatcgaa agcgagatcc accacgatct
      481 gatggcggaa atccacacgt gggcggagca gtgcccaaac gggggaaaga tcatccacct
      541 cggagccacc agcatggacg tcctggacaa catggatgcc ctccgcctta cagaagcgct
      601 ggatctgacg atcagcaaga cccgggagct gctcatcctc ttcaaggaga agatggaagc
      661 ctaccgggaa cttcccacga tggcgttcac ccacatccaa cccgccgagc cctccaccgt
      721 cggctaccgc ttcgcccaaa gcgcccaaga cctgaaggag gatctggagg agctgatccg
      781 ggtccgctcg tcgatccggg gcaagggggt gaagggggcg gtcggaaccg ccgccagcta
      841 tgcccagctt ctggagggaa ccggcctcac cgccatcaag cttgaagagg cggtcatgga
      901 agagatcgga cttgcggcat acaccgcttc gacgcagatc tacacccgca agcaggacct
      961 tcgcgtcgcc catgccctct cgggccttgc cgccaccctt tcaaagttct tcttcgattt
     1021 ccggattctc atgagccccc cgatcggcga gtggagcgaa tacttcgcct ccaaacaggt
     1081 cggctcctct gcgatgccct tcaagcgcaa cccgatcaac agcgaaaaga tcaacagcct
     1141 ttgccgcctc gtctcgaccc atgtcgacct tttctggcag aacgccgcga ccaccgttct
     1201 ggagcggagc ctggatgact cggcgaaccg ccggatcgcg atccccgaga tcttcctcgc
     1261 caccgacgag atcctcgtca ccgcaatcag gacggtaggc cggatgacgg ttcatgagga
     1321 agcggtcgga aggaacctca acgcgtacgg cctctttgcg gcaagcgagc gcctcctcat
     1381 ggagcttggc cgccgcggag cggaccgtca ggcgatgcac gaattgatcc gcaaccacag
     1441 cctgaaagcg tgggaggcgg tccgaagcgg caaggaaaat ccccttgcca agtcgctgag
     1501 cagggacaag acggttttgg ccttcgtcac cgaggaagaa gttcttgaat tcctgaaggt
     1561 cgaagcgtat atcggcgatg cggtcctgag gacgacgatg gtcgctaaag agatcgatgc
     1621 gttcctcaac cgagactgag gaatccgctg aagatcagga agtacagccc catccggaca
     1681 tagcggatct gcagggcgag caggaccctg aggatcggca gcttcgccat cccggcaagg
     1741 gtgcagatcg tggagaacgg cagcggcgcg atccccccga tcgccacgcc ccacgcgccg
     1801 t
//
LOCUS       DAOPUZ010000290         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_72219_length_1798_cov_3.854274, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000290 DAOPUZ010000000
VERSION     DAOPUZ010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72219_length_1798_cov_3.854274"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKN79_05230"
     CDS             complement(<1..816)
                     /locus_tag="PKN79_05230"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNZ95199.1"
                     /translation="MTSHHPIFITDALTLKGNLFLAPLAGYTDAPFRQLCIEGGADFT
                     YTEMVSVEGLARKGEKTIALLPRAKDEKEYAIQLFLGELASLRSALAIVKEHQPTMID
                     INAGCPVPKVTKTGAGSALMKNPPLIREIVRIIKEESGIPTSVKFRIGWDHESRNYLE
                     FADAAIEGGADLLTLHARTRAQGYAPFAQWDCLTSLKIHLAKKGLTIPLIGSGDLFEA
                     EDAVRMLQETNIDGVMFARGAVGNPVIFNQAKALLEGKEKPLLTATDKVNLLLT"
     gene            complement(813..1592)
                     /locus_tag="PKN79_05235"
     CDS             complement(813..1592)
                     /locus_tag="PKN79_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HNZ95200.1"
                     /translation="MKLFDTHAHIGLIQDDQMSQLLAVQMAKVKGVTHVVSITNSLAD
                     FEKTYENLKSNDSVLHAVGVSPTEVLNPGQNWEERVMANAKRDRVIAIGETGLDYHHY
                     AQARSQQIELMLKHLDIARILEKPIIIHNRDAKDDLLPILFEKLPPKGGILHCFGEDW
                     AFARKVLDLDLYFSFAGNLTFKHNRNLHEVAYRLPLERILIESEAPFMTPHRYKGERN
                     KIHYLVETLYAIAEIRDMAVEDVSEVIYDNSFRAFALKQES"
BASE COUNT          387 a          475 c          479 g          457 t
ORIGIN      
        1 ggtcagcagg agattcactt tatcggttgc ggtcaagagc ggtttttcct ttccttccaa
       61 aagcgctttt gcctgattga agattaccgg gttcccgacg gcccctcggg cgaacatcac
      121 cccgtcgatg ttcgtctctt gaagcatccg aaccgcatct tccgcttcaa agagatcgcc
      181 cgatccgata agaggaatgg tcaatccttt ctttgccaga tggattttca acgaggtcaa
      241 acaatcccac tgcgcaaaag gagcgtagcc ttgggcgcgg gtccgggcgt ggagggtcag
      301 aagatccgca cctccttcaa ttgccgcatc ggcgaactca aggtagttcc ggctctcatg
      361 atcccatcct atccggaact tcaccgaggt ggggattccg ctctcttcct tgatgatccg
      421 gacgatctcc ctgatcagtg gcgggttttt catcaatgcc gatcccgctc ccgtcttggt
      481 cactttgggc acggggcagc ccgcgttgat gtcgatcatc gtcggttgat gctcttttac
      541 gatggcaaga gcggatctga gggaggcaag ctcacccagg aagagttgaa tcgcatactc
      601 cttctcatcc tttgcccggg gcagcagcgc gatcgttttt tccccctttc gggcaagccc
      661 ttccacgctg accatttcgg tataggtgaa atccgcccct ccttcgatgc acaactggcg
      721 gaagggagca tcggtatacc ccgccaaagg ggcaaggaag aggtttcctt tgagggtgag
      781 ggcatcggtt atgaatatcg ggtggtgact ggtcatgact cctgcttgag ggcgaatgcc
      841 ctgaagctgt tatcatagat aacctcggaa acatcttcaa ccgccatgtc ccggatttca
      901 gcgatcgcat acaacgtttc aacgaggtag tgaatcttgt tgcgctcccc cttgtagcga
      961 tggggcgtca tgaacggcgc ctcgctttca atgaggatgc gttccagcgg caagcggtag
     1021 gcgacctcat ggaggttccg attgtgtttg aaggtaaggt ttcccgcaaa ggaaaagtac
     1081 aaatcaagat ccaacacctt ccgggcgaac gcccaatcct cgccgaagca gtgaaggatg
     1141 ccccccttgg gcggaagctt ttcaaagaga atcggaagca gatcatcctt tgcgtcgcgg
     1201 ttgtgaatga ttatcggctt ttccaggatt cgggcgatat ccagatgctt cagcatcagc
     1261 tcaatctgtt ggctccttgc ctgggcgtag tggtgatagt cgagtcccgt ctccccgatg
     1321 gctatgaccc gatcccgctt ggcgttggcc atgacccgct cttcccaatt ctgtcccggg
     1381 ttgagaacct ccgtgggcga gactcccacc gcatggagaa ccgaatcgtt ggatttgagg
     1441 ttttcatagg ttttctcaaa atcagcgagg ctgttggtga tcgagacgac atgggtgacg
     1501 ccttttacct ttgccatttg cactgccaac agctgactca tttgatcgtc ctgaatcagt
     1561 ccgatgtgag catgcgtgtc gaataacttc ataccaactc cttgagaaga ttcgctatcg
     1621 ggaattcttt caccataata ccatggggta tttcagtcgt caacgttgca atttctaagc
     1681 tatcaaacaa ttacgatgtg gtagtttgcg tttgacaatt tcaatggtgc gtggtagttt
     1741 tacagcgata tgaacaaaga ccacttgcgg gggagcaggg tgagtcaaca gtcggacc
//
LOCUS       DAOPUZ010000291         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_72230_length_1798_cov_3.292599, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000291 DAOPUZ010000000
VERSION     DAOPUZ010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72230_length_1798_cov_3.292599"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PKN79_05240"
     CDS             complement(<1..588)
                     /locus_tag="PKN79_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ95201.1"
                     /translation="MELFSVTKSGSEPLAYRMRPRTLEEYVGQEAIIGEGRLLRRAIQ
                     ADQLSSVIFYGPPGTGKTTLARVIANSTKRHFATLNAVLSGVKELRFEIDAARERLEL
                     YQRGTILFVDEVHRWNKSQQDALLPWVENGTVILIGATTENPFFEVNAALVSRSRIFQ
                     LKPLIGDDLMKIAHMTLSDKERGYGNYEITFEEGAL"
     gene            complement(588..1250)
                     /locus_tag="PKN79_05245"
     CDS             complement(588..1250)
                     /locus_tag="PKN79_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95202.1"
                     /translation="MTRRAKLITTILILFLVLLGGCTSTKQVKPLGGEQLYRELANTL
                     VEAAYGMDTTTLMAKTVEELLPANALPMNRIAGIPLFEEQLSRFKDEVNTAFLSTAWE
                     IAPLLEPIIQTIPWSDDLALVASLRSGVATLQRESRDELLSVITPILSDALKPAASRW
                     TALIDWYHLYRESVKLLNIEVLPALDEDIKEHLTLVYLERYCAALGDEEHRLRSQTTE
                     VR"
     gene            1447..>1798
                     /locus_tag="PKN79_05250"
     CDS             1447..>1798
                     /locus_tag="PKN79_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HNZ95203.1"
                     /translation="MQEQKMMHTAIARERTILKNVYLWMTAGLALSGLVAWLVAGSPA
                     LLRAMVGNPLSFLILVIAQFSLVFYLTARLDRMSASSAIGAFLAYAALNGAMLSTIFI
                     VYTQALIARAFFTTA"
BASE COUNT          365 a          476 c          518 g          439 t
ORIGIN      
        1 caacgccccc tcttcaaagg ttatctcata gttgccgtag ccgcgttcct tgtcactcaa
       61 cgtcatatgg gcgatcttca tcagatcgtc cccgatcaac ggcttgagct gaaagattct
      121 tgagcgggac accagggcgg cattgacttc aaagaagggg ttttccgtcg tggcgccgat
      181 caggatgacc gtcccgtttt ccacccaggg aaggagggcg tcctgctgac tcttgttcca
      241 gcgatgcacc tcatcgacga agaggatcgt ccctcgttga tacagttcaa gacgttcgcg
      301 tgccgcatcg atctcaaagc ggagctcctt cactccgctg aggacggcgt tcaaggtggc
      361 gaagtgccgc ttggtcgagt tggcgatcac ccgggcgagg gtggtttttc ccgtccccgg
      421 aggtccgtag aagatgaccg aggagagctg gtcggcctgg atcgcccgtc tgaggagccg
      481 tccttccccg atgatcgctt cctgacccac atactcctcc aaagttcggg gtctcatccg
      541 gtatgccagc ggctcggacc cggatttggt cacggaaaac agttccatca ccgtacctcg
      601 gtcgtttggc tgcggaggcg atgctcttca tcgccgaggg cggcgcaata gcgctccaga
      661 tatacaagcg tcaaatgctc cttgatgtcc tcatccaacg cgggaagaac ttcaatgttc
      721 aagagtttga cgctttcccg atagaggtga taccaatcga tgagagctgt ccatcggctt
      781 gccgccggct tcagggcatc gctgagaatc ggggtgatga ccgataggag ttcatcccga
      841 ctctcccgtt gaagggtggc gactcctgag cggagagatg cgacgagggc gaggtcatcg
      901 ctccagggta ttgtttggat aatcggctcc aagaggggag cgatctccca agcggtggaa
      961 agaaaggccg tattcacttc atctttgaag cgggagagct gttcttcaaa cagggggatg
     1021 ccggcgatcc ggttcatggg aagggcgttt gccggaagga gctcctcaac ggttttcgcc
     1081 atgagggtcg tcgtgtccat tccatacgcc gcttcgacca atgtgttggc aagctcccga
     1141 tagagctgct cgcccccaag gggcttcacc tgtttcgttg acgtgcatcc gccaagcagg
     1201 acgaggaaga gaatgagaat ggttgtaatc agcttggccc ggcgagtcat ctcagttcag
     1261 ctccttataa cagtgaagat catcttcatt caaaactata tgctttattt acgataagcg
     1321 caaagcatct tttggagaac gcctgccgag gaggctcttt ctttgtgaag tttccgtgaa
     1381 gagtgtgatt ctccattgac ctttgggtga tttgtgctca cattaaaggt atattggagg
     1441 tggatgatgc aggaacagaa gatgatgcac accgcgatcg cccgcgagcg gacgatatta
     1501 aaaaatgtat acctatggat gaccgccggt cttgctctca gtgggcttgt cgcctggctt
     1561 gtcgccggca gcccggcgct cctcagggcg atggttggga accctttgag ctttcttatt
     1621 ttggtcattg cccaattttc tctggtcttc tatttgaccg cccggctgga tcgaatgagc
     1681 gcttcgagcg caatcggagc gtttttggcg tatgccgcgt tgaacggagc gatgttgagc
     1741 acgattttca tcgtttacac gcaggccctg atcgcccggg cgtttttcac cacggctt
//
LOCUS       DAOPUZ010000292         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_72232_length_1798_cov_3.267355, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000292 DAOPUZ010000000
VERSION     DAOPUZ010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72232_length_1798_cov_3.267355"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..910
                     /locus_tag="PKN79_05255"
     CDS             143..910
                     /locus_tag="PKN79_05255"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95204.1"
                     /translation="MNTSSTSYTPIDLPSLAKKVNPKLAKVIPRCMYRKLERLLHVKE
                     INEFFAAHHDDDTRTFLDAVVSHLDLKIVIEGQGIEALHALEGERVIFASNHPYGGPE
                     AMALFSIVHQLFPDAKLVAQSFLKFIKPIETACVFNKKEVKTLMEAANNRSSLIFYPA
                     GYCSRILSNGEVFDYSWQSSFVRIARKQRMPILIIYTDGELSRKVHRWTRLRKLLRIK
                     LSIETIFLVDEMFKLKGKTLRMVVGSAITPEMIDEAK"
     gene            1202..>1798
                     /locus_tag="PKN79_05260"
     CDS             1202..>1798
                     /locus_tag="PKN79_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNZ95205.1"
                     /translation="MPMNMQYERAFGLDLSKKTFHGCILDGPDLGSRHFFTGEMGQQG
                     KAKLAGRLCKGDLVLMEAGTASFSLARFLLENTEAEVVVLNPAKLYNIFNSSLKNDKV
                     DSEKIARIARTMPRDEWPTVSIPTKEEQAERSAVSLEVFLAREITMLVNRLYALFNSM
                     GHPFIKKSDLKTEESRLTLANRLLCEDSLAIEQAIMLSD"
BASE COUNT          457 a          462 c          493 g          386 t
ORIGIN      
        1 ccgtgaggtc gtacggcgct ttttcagtga aaggcgcacg gttgaaggga tcgacatagc
       61 ggtcttgccg ccggcggtcg acttttacaa ggcgatcata aaaaaggtga gataaaagca
      121 aaaatgtgct aaaatacacg ccatgaatac aagctcgacg tcctatacac cgattgacct
      181 tccttccctt gcgaagaagg tcaatcccaa gcttgcaaag gtcatccctc gctgcatgta
      241 cagaaagctg gagagacttc ttcacgtcaa agagatcaac gagttctttg cagctcatca
      301 cgatgatgat acccggacgt ttctcgatgc agtggtctcc catctcgatc tgaagatcgt
      361 catcgaaggg caggggatcg aagccctgca cgctcttgaa ggggaacggg taatcttcgc
      421 ttccaaccac ccctatggag gaccggaggc gatggccctg ttctccatcg tccatcagct
      481 ctttcccgat gcgaagctgg tggctcaatc cttcctgaag ttcatcaagc ccattgaaac
      541 ggcgtgcgtg ttcaacaaga aagaagtgaa gaccctgatg gaagcggcga acaaccgcag
      601 cagcctaatc ttctaccctg ccggatactg ttcgcgaatc ctttccaacg gagaggtctt
      661 cgactacagc tggcagtctt cgttcgtccg aatcgcccga aaacagcgga tgccgattct
      721 catcatctac accgacggtg agctgagcag gaaagtccat cggtggaccc ggctacggaa
      781 gctcctcagg atcaagcttt caatcgagac gatcttcctg gtcgatgaga tgttcaagct
      841 gaaaggcaag accctcagga tggtcgtcgg atctgcgatc acgcccgaga tgatcgacga
      901 agcaaaatag aatgactact gtaaggatcg gagaggaact ctaatcttta cagctggagg
      961 ttcgatgata ttcttaactg acggagttag gaagccggtg gccgagtgtc ctgatttgta
     1021 aggattgggt gtagcccgag accttgaaag caaaaatatt ccaaaccggt catcgaagtc
     1081 tatcgtaggg catgtcgtgg aggctcaggc ctcccgatat gaccctgtaa ataaaaatgg
     1141 tggatcacga tccgtgtcca tacagccttc cagctctgaa tattcataag gaggtgctgc
     1201 catgccgatg aacatgcagt acgaacgggc attcgggttg gatctgtcga agaagacctt
     1261 ccacggatgc atcttggacg gccccgatct gggaagtcgc catttcttca ccggagagat
     1321 ggggcaacag ggcaaggcca agcttgcagg caggctttgc aagggggatt tggtgctgat
     1381 ggaggcgggg accgcatcgt tctcgctggc gaggttcctg ttggagaaca ccgaggcgga
     1441 ggtggtggtg ctcaatccag caaagctcta caacattttc aacagttcct taaaaaatga
     1501 caaagttgac agcgaaaaga tcgcgagaat cgccaggacc atgcccaggg acgagtggcc
     1561 cacggtctcg atcccgacca aggaggaaca agccgaacgg tcggccgtct ctcttgaggt
     1621 gtttctcgcc cgggagatca cgatgctggt caaccggctg tacgcactgt tcaactcgat
     1681 gggccatccc ttcatcaaga agtcggacct gaaaacggaa gagtccagat tgaccttggc
     1741 gaaccggctg ctgtgcgagg actcccttgc aatcgagcag gcaatcatgc tttccgac
//
LOCUS       DAOPUZ010000293         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_72246_length_1798_cov_2.699943, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000293 DAOPUZ010000000
VERSION     DAOPUZ010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72246_length_1798_cov_2.699943"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..>1320
                     /gene="aceE"
                     /locus_tag="PKN79_05265"
     CDS             145..>1320
                     /gene="aceE"
                     /locus_tag="PKN79_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNZ95206.1"
                     /translation="MLLARNVSSYVRWNAMAMVAKANRDYPGIGGHIASFVSYSAIYE
                     TGFNWFFKGPNAKYGADLVFFQGHSSPGMYARAFVEGRLTEDHLNHFRREVGGKGLSS
                     YPHPYLMPDFWQFPTVSMGLGPMMGIYQARFMKYLEARGFKEVGDRKVWVFMGDGESD
                     EPESLGALSLASRENLDNIIIVVNANLQRLDGPVRGNGKIIQELEGRFRGAGWNVIKM
                     IWGTEWDSILERDTKGVLLRKLNSMVDGEFQTIQSRGPAYLREKLFDDDYLKSLVADK
                     SDKELWQLTRGGHDPRKIYKAFHSAVNHTGEPTVIIFKTVKGYGMGSGEASMGAHNVK
                     EMDKESLLAFKERFHVPLADDQLTELPFIKPDPKSPEAEFLKRRREIMGGPVPYRNPD
                     "
     assembly_gap    1321..1330
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1331..>1798
                     /gene="aceE"
                     /locus_tag="PKN79_05270"
     CDS             <1331..>1798
                     /gene="aceE"
                     /locus_tag="PKN79_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608136.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNZ95207.1"
                     /translation="EIPSLDEFAEFLASSGKREISTTMAYVRMLTKLVKDKKIGRHIV
                     PIVPDEARTFGMEGLFRQLGIYSPAGQLYEPQDSESLMWYHEERDGQILEEGITEAGA
                     ISSWIAAATSYANNGVCMIPFYTFYSMFGFQRIGDFVWAAGDMHAKGFLMGGTA"
BASE COUNT          393 a          527 c          546 g          322 t
ORIGIN      
        1 cgaagggcca cagaaggcga accacctgct gatcgaattg atcgcttcgg cgagaaacca
       61 gggggttcga accagtccgg gcgtcatcag cccctatgag aacacgatga tcaatgaacc
      121 ggaagcgatc atccccgccg acgattgctc ctggcgagaa acgtcagctc ctatgtccga
      181 tggaacgcga tggcgatggt ggcgaaagca aaccgcgact atcccgggat cggaggacac
      241 atcgcctcct tcgtttccta ctcggcgatc tatgagaccg gcttcaactg gttcttcaag
      301 ggtcccaatg cgaaatacgg ggcggatctg gtgttttttc agggacacag ctccccgggg
      361 atgtacgccc gggcatttgt cgaaggccgt ttgacggagg atcacctgaa ccacttccgc
      421 cgggaagtcg gaggaaaggg gctttcctcc tacccccacc cctatctgat gcccgacttc
      481 tggcagttcc ccaccgtcag catgggcctt ggcccgatga tggggatcta tcaggcgcgc
      541 tttatgaaat accttgaagc ccgcggattc aaggaggtcg gcgaccgaaa ggtctgggtg
      601 ttcatggggg acggtgaaag cgatgagccg gagtccctcg gagcgctctc cctcgcatca
      661 agagaaaacc tcgacaacat catcatcgtg gtgaacgcca acctccagcg cctggacggt
      721 ccggtccgcg gcaacggaaa gatcatccag gagctggaag gccgcttccg cggtgccggc
      781 tggaacgtca tcaagatgat ctggggcacg gagtgggatt cgatccttga gcgcgatacc
      841 aagggtgtgc ttctcaggaa gctcaattca atggtcgacg gcgagttcca gacgatccaa
      901 tcccgcggac cggcgtatct tcgcgagaag ctcttcgatg acgactacct caagagcctt
      961 gtcgccgaca agagcgacaa ggagctgtgg cagctcaccc ggggcggaca cgacccccga
     1021 aagatctaca aggcgttcca ttccgcggtc aaccatacgg gggagccgac ggtcatcatc
     1081 ttcaagacgg tcaaggggta tggaatgggc agcggtgagg cctcgatggg agcgcacaac
     1141 gtcaaggaga tggacaagga aagcctgctc gccttcaagg agcgcttcca cgtcccgctt
     1201 gcggacgacc agctgaccga gctgccgttc atcaagcccg atccgaagag ccccgaggcg
     1261 gaattcctca agcgccggcg agagatcatg ggcgggccgg ttccctaccg gaatcccgac
     1321 nnnnnnnnnn gagattcctt cactggatga gtttgccgag ttcctcgcct ccagcggcaa
     1381 gcgcgagatc tccacgacga tggcgtatgt ccgaatgctc accaagctgg tgaaggacaa
     1441 gaagatcggc cgacatatcg tcccgatcgt ccccgacgag gcgcgaacct tcggcatgga
     1501 ggggcttttc aggcagcttg gcatctactc ccccgccgga cagctctacg agccgcagga
     1561 ttcggaatcc ctgatgtggt accacgaaga gcgggacggc cagattctgg aggaaggaat
     1621 caccgaagcg ggggcgatca gctcctggat cgctgcggcg acgagctacg cgaacaacgg
     1681 cgtgtgcatg attcccttct acaccttcta ttcgatgttc ggattccagc ggatcggcga
     1741 cttcgtgtgg gcggcgggcg acatgcacgc caaaggcttc ctcatgggag gaaccgcc
//
LOCUS       DAOPUZ010000294         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_72618_length_1792_cov_2.866436, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000294 DAOPUZ010000000
VERSION     DAOPUZ010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72618_length_1792_cov_2.866436"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKN79_05275"
     CDS             complement(<1..235)
                     /locus_tag="PKN79_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNZ95208.1"
                     /translation="MNKPKRTFLGELKGWADALVFAVFAVLLINQYLFQLFVIPTPSM
                     VSTLDIGDRVFVSKTIYGVELYPTGPKVGSKNRQ"
     gene            383..697
                     /locus_tag="PKN79_05280"
     CDS             383..697
                     /locus_tag="PKN79_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95209.1"
                     /translation="MKQKIDLPPVEEVVLPKLFNLRPGYYLLGLMILVVLLLIFLLGF
                     LPGIRKGGRYVTFEAPLSETGILLDGKYLGSTTHQYFVPSGNHTIACQSRSDLCRNGD
                     TR"
     gene            831..1718
                     /locus_tag="PKN79_05285"
     CDS             831..1718
                     /locus_tag="PKN79_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ95210.1"
                     /translation="MEYPPQFPYQPVYANLHNDLEALQLRNSQPIIDLALSFISNDTM
                     KSDAERFFPVETKSAPVQAGIQELPSVGKPITLDAGNLTIEGYSYTGSSFIMGDGITP
                     QSGYAPVSTKDFVLAKRPVSQYEWALFVQENPQWAKSAMNGSSYLAGLSLSPQFSTNR
                     PIYNVSYHAARAFVQWLGEKSGKEVFLPTEAMWSQAAYSQKHTEYDTSLALSERSVPL
                     LGLLGGVWEMTDSHYIPNGRNAGYDRLQELAETYGLGKQVIVKGGSMISKDVTIDSVG
                     VIEPDSVHDYIGFRIGWFE"
BASE COUNT          450 a          467 c          453 g          422 t
ORIGIN      
        1 cctgtcggtt ttttgaaccg accttcggcc cggtgggata cagttcgact ccgtaaatgg
       61 tttttgagac gaacacccga tcaccgatgt ccagcgtcga aaccatcgaa ggcgtaggaa
      121 tcacgaagag ctgaaacaga tactggttga tcagcaaaac tgcgaagacg gcgaagacca
      181 atgcatctgc ccaccctttc agctcgccca ggaaggtgcg tttcggctta ttcatcttgt
      241 gatggaggcg aactgccttg cgatgggtaa gatatcgaac ggtcaaactc tccaggcggg
      301 ttaagaatcg gtccatatga ggcaaggtat cacaacgggt attggttgac aaggcaatga
      361 gggatggcta ccttacttgt gtatgaaaca aaagatcgat cttcctccgg tggaagaggt
      421 ggttctcccg aaattattca atctgagacc cggatattat ctcctcggtc tcatgattct
      481 cgtcgtgctg ctcttgatct ttcttttggg atttcttccc ggaatccgca aaggcggccg
      541 atacgtgacg tttgaggcgc cgttaagtga aacgggaatc ctgctggatg ggaagtattt
      601 ggggtcgacg actcaccaat acttcgttcc cagcggcaac catacgatag cctgtcaaag
      661 ccgatcagat ctatgccgaa acggagatac acgttgacca tcccgttttt cttgcgaatc
      721 tgattcaccg aacacgggta attcccccac caccggtcac ccttaccgaa gaagaaacag
      781 cttcgatcgt ttcctttctc ctcaatgaag ttcaggagat cagcaaatcg ttggaatatc
      841 ctccccaatt tccctatcaa ccggtctatg ccaatctcca caatgacttg gaagctctgc
      901 aactaaggaa tagccaacca atcatcgatc tcgccctgag cttcatcagc aacgatacga
      961 tgaagagcga tgcggaacgg ttctttcctg tagaaaccaa atccgctccg gtccaagccg
     1021 gtattcaaga gctcccgtcg gtcggaaaac cgattaccct cgatgcgggc aaccttacga
     1081 ttgagggtta ctcctatacc ggttcatcct ttatcatggg ggacggaatt acaccgcaaa
     1141 gcggttacgc acctgtctct accaaggatt tcgttttggc caagcgcccg gtatcccagt
     1201 atgaatgggc gctcttcgtt caggagaatc cccaatgggc gaaaagcgcg atgaacggtt
     1261 cctcatacct cgccggcctc tccctgtcac cacagttttc aaccaaccgg ccgatctaca
     1321 atgtcagcta tcatgcggcc cgggcatttg tccaatggct cggtgaaaaa agcggtaagg
     1381 aggtgtttct tccaactgaa gcgatgtgga gccaggcggc atacagccag aaacataccg
     1441 agtatgatac gtcgctcgct ctttccgagc gctccgttcc cctccttggt cttcttggag
     1501 gagtctggga gatgacggac agccactaca ttccgaatgg gagaaatgcc ggctatgatc
     1561 gcctgcagga gcttgccgag acctatggtc ttgggaagca ggtgatcgtc aagggcggca
     1621 gtatgatcag caaagacgtc acgatcgatt cggtgggcgt aatcgaaccg gatagcgtac
     1681 atgattacat cgggttccga atcggatggt ttgagtgatg aagaactcca aggatcacat
     1741 caagatgctc caaaagaacc gcaaggcata cttcaactat gaggtggttg aa
//
LOCUS       DAOPUZ010000295         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_72988_length_1786_cov_3.686886, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000295 DAOPUZ010000000
VERSION     DAOPUZ010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72988_length_1786_cov_3.686886"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /gene="mtaB"
                     /locus_tag="PKN79_05290"
     CDS             complement(<1..1269)
                     /gene="mtaB"
                     /locus_tag="PKN79_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNZ95211.1"
                     /translation="MRVCVYTLGCRLNQCESEAIADSFVKEGFEIVGEQDGADLYIVN
                     TCTVTSKAEQKARRMIRRFAQSSPTLVTGCYAQLNREELESLGKEVLVVSLDRKARLL
                     KLASHLRVAMESSFSLFEGMKSFTGEDATVFDYAAATFSYHSRAYLKIQDGCDNSCAY
                     CRVHVARGKAQSLDRAIVIERAKALEEAGFNEIMLTGVNLTMYDHRNEGLGGLLEALL
                     GELGDLRIRLSSMEPDHIDERLLGVLEDPRMQPHFHIPVQSGSDSVLKRVGRKYTIAE
                     LTAILDRLKTVKDDPFLAADVITGLPGEGEREFEETYMFLKEIGFSALHVFPFSPRPD
                     TPLFNAKDRVPESVRDERAKILRTLSAELTDAYLRRQEGKQGEVIVQGRKGGFWCGVT
                     GNYVDVRIVNPPLFAQRGMLLEGVFGKKADR"
     gene            complement(1266..>1786)
                     /locus_tag="PKN79_05295"
     CDS             complement(1266..>1786)
                     /locus_tag="PKN79_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNZ95212.1"
                     /translation="LKEGAVVMDTCSVKLYPARVMKRLLPPTIQAIATHPMFGPDSGR
                     DGLEGLPFVMDNISAREETFRWWIDEFTSWKMDVLMMSCDQHDREAAWSQGITHFIGR
                     TLAELNLGSTRLATKGYQTLMGVVEQTCNDPLQLFYDLQRYNPYTKQMRMGLRGALVT
                     TMEALKEQEEIL"
BASE COUNT          352 a          573 c          484 g          377 t
ORIGIN      
        1 gcgatcggcc ttcttgccga acacgccctc aaggagcatt ccccgctggg caaagagcgg
       61 cggattcacg attcttacat ccacgtagtt gcccgtcacc ccgcaccaga agccgccttt
      121 ccgcccctgg acgatgacct cgccttgttt cccctcctgc cggcgcaggt aggcatcggt
      181 gagctcggcg ctcaatgttc ggagaatttt cgcccgttca tcccggaccg actccggcac
      241 ccggtccttc gcgttgaaga gcggcgtatc gggccggggg gagaagggaa agacgtggag
      301 ggcgctgaag ccgatttctt taagaaacat gtaggtttcc tcgaactccc gctccccttc
      361 gcccggaagg ccggtgatca cgtccgcagc cagaaaggga tcgtccttga cggttttcaa
      421 ccggtcgagg atcgccgtca gctcagcgat cgtgtacttc ctgcctaccc gcttcaggac
      481 ggaatccgat ccgctttgca cgggaatgtg aaagtgcggc tgcatccggg gatcttccaa
      541 taccccgagc aggcgctcgt cgatatgatc cggctccatc gaggagagcc ggatccgaag
      601 atcgccgagt tctcccagga gcgcttccag cagcccgccg agcccctcgt ttcgatgatc
      661 gtacatcgtg aggttcaccc cggtgagcat gatctcgttg aagcccgcct cttcaagggc
      721 tttcgcccgc tcgatgacaa tggcccgatc gaggctttgc gccttgcccc gggcgacatg
      781 gacccggcag tatgcgcaac tgttgtcgca cccgtcctga atcttcaggt acgcccggct
      841 gtggtaggaa aaggtggcgg cagcgtaatc gaagaccgtc gcgtcttcac cggtgaaact
      901 cttcatccct tcgaagaggg agaatgaact ctccatcgcc actcgaagat gcgaagcaag
      961 tttcagaagc cgggccttcc tgtctagcga aacgaccagc acctctttgc cgaggctctc
     1021 aagctcctct cggttcagct gggcatagca gccggtgacc aatgtcggtg agctttgagc
     1081 gaatcggcgg atcatccgcc gggctttctg ctccgctttc gaggtgacag tgcaggtgtt
     1141 gacgatatag agatcggcgc cgtcctgttc accgacgatt tcaaaccctt ctttgacgaa
     1201 ggaatcggcg atcgcttcgc tttcacactg attcaaccgg catcccaggg tatagacgca
     1261 gaccctcaaa ggatctcctc ctgctccttc agcgcctcca tcgtcgtcac cagcgctccc
     1321 cgcaggccca tccgcatctg cttcgtgtag ggattatacc gctgcagatc ataaaagagc
     1381 tgcaaggggt cgttgcacgt ctgctccacc acccccatga gggtctggta ccctttggtc
     1441 gccaagcggg tcgaacccag attcagctcg gcaagcgtcc gcccgatgaa gtgggtgatt
     1501 ccctggctcc acgccgcttc acgatcgtgc tgatcgcagc tcatcataag cacatccatc
     1561 ttccacgacg tgaactcatc gatccaccat cggaaggtct cttcccgagc actgatattg
     1621 tccatgacaa agggcaagcc ctccagtcca tcccttcccg aatcgggacc gaacatcggg
     1681 tgggtcgcga tcgcttgaat cgtcggtgga agcaggcgct tcatcacacg agcgggatac
     1741 agtttaaccg aacacgtatc catgacgacc gccccttcct tcagat
//
LOCUS       DAOPUZ010000296         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_73051_length_1785_cov_3.767630, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000296 DAOPUZ010000000
VERSION     DAOPUZ010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73051_length_1785_cov_3.767630"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..>1785
                     /locus_tag="PKN79_05300"
     CDS             145..>1785
                     /locus_tag="PKN79_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNZ95213.1"
                     /translation="MGIREEIRHRVLLLDGAMGTMIQSLRLPPSAFTYRGRTAAGLAE
                     VLNLTQGEAITSIHRAYIEAGADIIETNTFNANRISLSEYHLEDEVAPINRRAVALAK
                     EASRDAERRVYIAGVLGPTASGLSFSAKVDDPTARTHDFDFFLKAYREQAKALIEAGC
                     DLLLIETVFDTLVAKSAIIACREELEAAGKDLPIMVSVTFSDGSGRTLSGQSLGAFIA
                     TLAPFDLFSIGLNCSTGPKEMIPLLNELHAISPCYTSAHPNAGFPDEDGRYTLAPDHL
                     AGELKPLLAEGFLNIVGGCCGTTPDHIRALKRILPASRPRSVPPKKPALLLAGLDLVE
                     VKRDTLLIVGERTNVAGSRIFRRRIERKQWEEALLVARDQIAEGADLLDICMDDPLLD
                     SKEAIVSFLRYIASDPTIAKAALMIDSSDWSVIEAALGEVQGRGIVNSISLKEGEELF
                     IRRAKKIASFGHAFVVMLFDEAGQAASYERKLAIAERSYTLLVEAGIEPSSIIFDPNV
                     LAIGTGIAEHDAYAKATIDATRELKKRYPRSYISGGISN"
BASE COUNT          376 a          536 c          519 g          354 t
ORIGIN      
        1 tgcttgagaa ggcggaggag tggcgcgtgg agatgatcgt ttcaaatgaa cttgtcaacg
       61 aggtcgtcga agcgctgctt tccgcccacc cgtatgaaat gcccgcctac catctctacc
      121 ccgtattgac caaggagagc tttgatgggc attcgtgaag aaatccgaca ccgggtcctg
      181 ctcctcgacg gggcgatggg tacgatgatc caaagtcttc ggctgcctcc ttccgccttc
      241 acctatcggg gaagaaccgc cgcgggcctt gccgaagtct tgaatcttac ccaaggtgaa
      301 gcaatcacct cgattcaccg ggcgtatatc gaagcgggtg cggacatcat cgagaccaat
      361 acgttcaacg ccaatcggat cagtttgagc gagtatcacc ttgaagacga agtggcgccg
      421 atcaaccggc gggcggtcgc cctcgccaag gaagcgagca gggatgccga gcgaagggtg
      481 tatatcgccg gagtgctcgg tcccacagct tccggccttt ccttttccgc caaagtggat
      541 gatcccaccg cccgaaccca tgatttcgac ttcttcctta aggcctaccg cgagcaggcc
      601 aaagccctca tcgaagcggg gtgcgatctc ctgctcattg aaacggtttt cgacacgctg
      661 gttgccaaaa gcgcgatcat tgcctgccgg gaggagctcg aagcggcagg caaagacctt
      721 ccgatcatgg tcagcgtcac gttcagcgac ggcagcgggc gaaccttgag cggtcagagc
      781 ctgggggcgt tcatcgccac cctcgccccc ttcgatctct tcagcatcgg gctgaactgc
      841 tccaccggac cgaaggagat gattcctctc ctaaacgaac ttcacgcgat ctccccctgt
      901 tatacgtcgg cccacccgaa cgccggtttc cccgatgagg acggcaggta caccctcgct
      961 cccgatcacc ttgccggaga gctgaagccg cttcttgcag aggggttcct gaacatcgtc
     1021 ggagggtgct gcggaacgac ccctgaccac atcagggctc tcaagagaat ccttcctgca
     1081 agcaggcctc gatcggttcc accgaaaaag cccgctctgc tgttggcggg actggatctg
     1141 gtcgaggtga agagggatac cctgctcatc gtgggcgaac ggacgaatgt cgccggatcg
     1201 cggatcttcc gccgccgcat cgaacggaaa caatgggaag aagccctcct cgtcgcccgg
     1261 gatcagatcg ccgaaggtgc cgatcttctg gatatctgca tggatgatcc gctcttggat
     1321 tccaaagagg cgattgtctc gttcttacgg tatatcgcca gcgaccccac gatcgcaaaa
     1381 gcggccctga tgatcgattc ctccgactgg tcggtcatcg aagcggcgct tggggaggtc
     1441 caggggcggg ggatcgtcaa ttcgatctcc ctgaaggaag gagaggagct cttcatccgc
     1501 cgggcgaaaa agatcgcttc cttcggccat gcgttcgtgg tgatgctctt tgacgaagcg
     1561 gggcaggccg caagctatga acggaagctt gcgatcgccg agcgttctta caccctcctt
     1621 gtcgaggcgg gaatcgagcc ctcttcgatc atcttcgatc cgaacgtgct ggcgatcgga
     1681 accggcatcg ccgagcacga cgcctatgcc aaagcgacga tcgatgccac ccgggaactg
     1741 aagaagcggt atcctcgttc gtatataagc gggggaatca gcaac
//
LOCUS       DAOPUZ010000297         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_73136_length_1784_cov_2.598612, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000297 DAOPUZ010000000
VERSION     DAOPUZ010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73136_length_1784_cov_2.598612"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..841)
                     /locus_tag="PKN79_05305"
     CDS             complement(5..841)
                     /locus_tag="PKN79_05305"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HNZ95214.1"
                     /translation="MEQQKPLATREVQLLELEILKAFDAFANKHSIPYHLSYGTLLGA
                     VRHKGFIPWDDDIDVLVPRPEYERLLDLLEREGLGKRFTVASPRNRSKTYPYPYAKLL
                     LNDTVVYEPKLDSKFSKSSIWIDIFPLDGLPNTKARRTMTFFWALALKNLLYTAIVSP
                     KHVSGIQKLAVLLLGPITRMIGIGRWVDWTERFATRHSYDEAKLVGNLVLTEGSQEAI
                     AKAMISETITLEFEGHPFPAPKHYDEYLTQLYGAYMELPPVEQRTTHLGNQAFLAETG
                     HV"
     gene            963..>1784
                     /locus_tag="PKN79_05310"
     CDS             963..>1784
                     /locus_tag="PKN79_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95215.1"
                     /translation="MKKAIALVLLISVLLTGIFAISHLSVPLGDRVYEILRSAELRGM
                     IDRVDTVKPYPRSIVVKHLEAILQHPDVKASERTEIMQILKRFDNSFTSPKGFNEALT
                     LGGYQSYWDEYKTGVVFGVEAGTTITKSLTVSDSYDARIRLRPYMRGDISDFLSFQMD
                     FGLVFDRLDSRLFPLNDFTFDTDGKYDSIFNIAEEHRFYYGQFNEPELALSFLGGNLQ
                     LRWGAMARDWGVARNNLMIAGSARPFQGIETSITFTSWLNYQFITGTLGKFSPNSL"
BASE COUNT          398 a          448 c          476 g          462 t
ORIGIN      
        1 cttctcacac atggccggtc tccgcaagaa atgcctgatt tccgaggtgg gttgttcgtt
       61 gttcgacggg tggcagctcc atatatgctc catagagctg agtaagatat tcgtcatagt
      121 gtttgggagc ggggaaggga tgtccttcaa actcaagagt gattgtttcg gatatcattg
      181 cttttgcaat tgcttcctgg cttccctcgg taagtacgag attgcccacc agcttcgcct
      241 catcatagga gtgtcgggtg gcgaaccgtt cggtccaatc cacccatctc ccgattccaa
      301 tcatccgagt gatcgggccc aagagcaaga ccgccaattt ttgaattccg gaaacgtgtt
      361 tcggtgagac gatcgccgta tagagcaggt ttttcaaggc caacgcccaa aagaacgtca
      421 tggttctgcg tgcttttgtg tttggcagcc cgtcaagggg aaagatgtcg atccaaatcg
      481 atgatttgct gaatttggaa tcgagcttcg gctcatagac gaccgtatcg ttgaggagga
      541 gctttgcata cggatacggg taggttttgg atcggttccg gggggaggcg acggtaaatc
      601 gttttcccaa accttctctt tccaggagat ccagaaggcg ttcatactcg ggccggggaa
      661 caaggacgtc gatatcatca tcccaaggga taaacccttt gtggcgcacc gccccgagaa
      721 gggtgccgta ggagaggtga tagggtattg aatgcttgtt tgcaaatgca tcgaacgctt
      781 tgagaatctc aagctcaagc aactgaacct cgcgggtggc aagcggtttc tgttgttcca
      841 cggagtgctc ctctctgtag ggactgtacc acacccgccg gggaataatc cacctctgct
      901 tgacagagtg agccctcgct ttcatagtaa gagttgcgta ttgaccttaa ggagttttat
      961 tcatgaagaa agcgattgca cttgtccttc tcatatcggt tttgctaacc ggtatttttg
     1021 ccataagcca cctgtccgtc ccgttgggag accgggtata tgaaattctt cgttccgccg
     1081 aattgagggg aatgatcgat cgggtagaca cggtcaaacc ctatccccgt tcaatcgtgg
     1141 tgaaacatct tgaagccatt cttcaacatc cggatgtgaa agcctccgaa cggacggaga
     1201 tcatgcagat cctcaagcgg ttcgacaact ccttcacttc gccgaagggt ttcaacgaag
     1261 ctctcacctt gggcggctat caaagctatt gggatgaata caagacgggt gttgtcttcg
     1321 gggtggaagc cggcaccacc ataacgaaat cgctgacggt atcggactcc tacgatgccc
     1381 gaatccggct tcgaccctac atgcggggcg acatctccga tttcctttcc ttccagatgg
     1441 acttcggtct ggtttttgac cgtcttgaca gccggttgtt tcctctgaac gattttacat
     1501 ttgacaccga tgggaagtac gattctattt tcaatatagc ggaggaacat cgcttctact
     1561 atggacagtt caacgagccg gagcttgcct tgagcttcct tggcggaaac ctgcagctcc
     1621 gctggggggc gatggcgcgg gattggggtg ttgctcgaaa caacttgatg atcgcgggat
     1681 ccgcccgacc gtttcaggga atcgagacct caatcacctt cacctcgtgg ttgaactacc
     1741 agttcatcac cggaacgctt gggaagtttt cgcccaattc cctc
//
LOCUS       DAOPUZ010000298         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_73689_length_1775_cov_4.319186, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000298 DAOPUZ010000000
VERSION     DAOPUZ010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73689_length_1775_cov_4.319186"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PKN79_05315"
     CDS             complement(<1..130)
                     /locus_tag="PKN79_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNZ95216.1"
                     /translation="MLYLQLFLSFFQIGLFSIGGGYVAMPLLQHQTVDLHGWLTQAQ"
     gene            complement(130..741)
                     /locus_tag="PKN79_05320"
     CDS             complement(130..741)
                     /locus_tag="PKN79_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNZ95217.1"
                     /translation="MKTEIKKGKPEVNVKFFLRLFWETFTLSMSGFGGGFVIVSLMRK
                     KMVAKLGWIDQDEMMDFVIIAQSAPGSASVNVAMMVGYHLARTPGALVATLGTVLPPM
                     IIVSIISVIYTILQGNVWVDAAFLGMRPVVAAIVLQVSWKFFKPFLKVKAIPAILLFI
                     ISFVLLYILKVSMIALILVMLALSTTANLLDMRRRKKRMEEDI"
     gene            complement(753..>1775)
                     /locus_tag="PKN79_05325"
     CDS             complement(753..>1775)
                     /locus_tag="PKN79_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNZ95218.1"
                     /translation="CFYLDTARNRSVHHHPVMNDEVAGKIIDKSMALARNIQFVFQGG
                     EPSLAGLSFFERFVSRVNERQGEGQRITWAFQTNGVGLDRSWADFFKAHGFLVGVSFD
                     GTRRLHDLHRVDRQGLGSGRSVLNTIDLFTKEGVDFNILSVVTNDLVDNVQMLYSFLN
                     EHGLHYQQYIPCIDPMQGRKRFLSAEGYERFLKAMFDLWFDSFMNQTPVSNRFFDNLV
                     GMIAGYPPESCDMAGICSVQYVFEANGDVYPCDFYCTDEHRLGTILFDEFSTLDERRR
                     GMRFIEESSNTIDGCETCPWKHLCRGGCKRYRNDDGYRFCSTMMGFFPYVIERLHYVA
                     KQITPP"
BASE COUNT          459 a          505 c          434 g          377 t
ORIGIN      
        1 actgggcttg ggtgagccag ccgtgaagat cgaccgtctg gtgttgaagc aacggcatcg
       61 ccacataccc gccgccgatt gaaaagagac cgatttggaa gaagcttaag aacagctgga
      121 gatagagcat catatgtctt cctccatccg cttcttcctc ctcctcatgt caaggaggtt
      181 ggccgtggtg gagagcgcca gcatcaccag aatcagtgcg atcatgctga ccttcagaat
      241 atagagcagg acgaaactga tgatgaagag cagaatcgcc ggaatcgctt tcactttgag
      301 aaacggtttg aagaacttcc acgaaacctg aaggacgatc gctgcgacca ccggtctcat
      361 cccgagaaac gccgcgtcga cccagacgtt gccctgaagt atggtgtaga tgacgctgat
      421 gatcgagacg atgatcatcg gcggcagaac cgtccccaac gtcgcgacaa gcgcacccgg
      481 agttcttgcc aggtgataac cgaccatcat cgccacgttc accgaggcgg atcccggcgc
      541 ggattgggca atgatgacga agtccatcat ctcatcctgg tcgatccagc caagctttgc
      601 gaccatcttc ttgcgcatca ggctgacgat gacaaatcct ccgccgaagc cggacatgct
      661 caaggtgaat gtctcccaga acaggcggag aaagaatttt acatttactt ccggtttccc
      721 cttcttgatt tcagttttca tgcagtaccc cactaaggag gagttatctg cttggccacg
      781 tagtgcaatc gttcaatcac ataggggaaa aaccccatca ttgtcgaaca gaagcgatac
      841 ccgtcatcat ttcgataacg cttgcaaccg cctctacaca agtgcttcca cggacatgtc
      901 tcacacccat cgatcgtgtt agaactctcc tcgatgaagc gcattccccg gcgtcgttca
      961 tcgagggtcg aaaattcatc gaaaaggata gttcccaatc gatgctcatc agtgcaatag
     1021 aaatcgcaag gatacacatc cccgttggct tcgaaaacgt attgcacgga acagatgccg
     1081 gccatatcgc aactctcagg gggatagccg gctatcatcc ccacgagatt atcgaaaaaa
     1141 cggtttgaga ccggggtttg gttcatgaat gaatcaaacc acaagtcgaa catggccttg
     1201 aggaatcgtt catacccctc ggcactcaaa aaacgcttcc tcccctgcat cggatcaatg
     1261 caggggatgt actgttgata gtgtagccca tgctcgttca ggaaggaata gagcatttgc
     1321 acattatcaa cgagatcgtt cgtcacaacg ctcaggatgt tgaagtccac cccttccttt
     1381 gtgaacagat caatcgtatt caacacgctc cggccacttc ccagaccttg gcggtcgacg
     1441 cgatggaggt catgaagccg gcgcgtccca tcaaacgaga cccccaccag aaacccatgg
     1501 gctttgaaga agtcagccca agaccggtcc aaccccaccc cgttggtctg aaacgcccag
     1561 gtgatccgtt gcccctcccc ctgccgttca ttgacgcgcg aaacaaagcg ctcgaagaat
     1621 gaaagaccgg ccaaggaagg ctcccctccc tggaagacga actggatgtt ccgagcaaga
     1681 gccatgctct tgtcgatgat cttgcccgca acctcatcgt tcatgaccgg gtgatggtgg
     1741 acgctcctgt ttctcgcagt atcgaggtaa aaaca
//
LOCUS       DAOPUZ010000299         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_73758_length_1774_cov_3.709133, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000299 DAOPUZ010000000
VERSION     DAOPUZ010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73758_length_1774_cov_3.709133"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKN79_05330"
     CDS             <1..591
                     /locus_tag="PKN79_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNZ95219.1"
                     /translation="YTVASLISFRNGNPDKPNYRRYNIKSVEGRIDDYDSIREAVARR
                     YQKVLNENLERPGLLLIDGGHGQVNAAREVLDDLGIFDIPVIGLIEGSETIVFGDGKE
                     DLRLDHGNEALRLLIAVRDECHRFAHASNQAARSRTASFQLLQSVDGIGKKRSEALMA
                     QYGSLEAVLKKDPEDLAKEAKLPLAVAQRLIKQLSL"
     gene            complement(586..1614)
                     /gene="holA"
                     /locus_tag="PKN79_05335"
     CDS             complement(586..1614)
                     /gene="holA"
                     /locus_tag="PKN79_05335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNZ95220.1"
                     /translation="MSSYLLLGPEAGAKADEVKRIRSELAAKYGDGLEFERFYPFDGE
                     QGELFASLANDSLFSDHRLILISQLESAGAAFLNELAGYLANPSPSATVVLASSETSI
                     TKKIMDAIPKERTRIFYDLLEGEKSAWIRTHFRRLGLTITSEAVELLIAMTEDNTQEL
                     RTVCNQLALFWQHDSPTKPIDEEAVQTYLHHSRVEDAFTLFPHLAAGDLKKALAGLHA
                     LLGSGDYQTPILLVSGLLWQFRRLLSVAEGFEEGKDEPEVFSNAHVLLKNSAIRRPKD
                     KNTYREGAKHYSSKDIRSIIIALAQADIEVKQSRSEEIPLILERLIYRIINKKGKELT
                     TASFATLL"
BASE COUNT          368 a          487 c          524 g          395 t
ORIGIN      
        1 tacaccgtcg catcgctcat ctcgtttcga aacggaaacc ccgataaacc gaactatcga
       61 cgctacaaca ttaaaagcgt cgaaggacgg atcgacgatt atgattcgat ccgcgaggcg
      121 gtcgcccgca ggtatcagaa ggttctcaac gaaaatctgg aacggccggg tctgctgctc
      181 attgacggag gacatgggca agtcaatgcg gcccgggaag tccttgacga tctggggatt
      241 ttcgatattc ccgtcatcgg gctgatcgaa gggtcggaga cgatcgtctt cggggacggg
      301 aaagaggatc ttcgcctcga tcacggcaat gaagcgctga ggctcctcat cgccgttcgg
      361 gatgagtgcc atcgcttcgc ccacgcctcc aatcaggccg cccgctcccg caccgcttcc
      421 ttccaattgc ttcagtccgt cgatggaatc ggaaaaaaac gaagtgaggc gttgatggct
      481 caatacggaa gccttgaggc ggtgctgaaa aaagatccgg aggatctggc caaagaggcg
      541 aagctgccgc ttgccgtcgc tcaacggctg attaaacagc tctccctata gcagtgtcgc
      601 gaaggatgcc gtagtgagct cttttccctt cttgttgatg atccggtaga tcagccgctc
      661 gaggatgagg ggtatctctt cgcttcggct ttgtttcacc tcgatatcgg cttgggcgag
      721 ggcgatgatg atcgaacgga tatccttcga ggagtagtgc ttcgctcctt cccgataggt
      781 gttcttatcc ttcggacgcc ggatggcgct gtttttcaga aggacatggg cgtttgaaaa
      841 cacctcgggt tcatccttgc cctcttcaaa gccttcggcg accgaaagca aacgtctaaa
      901 ttgccacaac aagccgctga cgagcagaat gggagtctgg taatcaccgc tgcccaacag
      961 ggcgtgaaga ccggcgaggg cttttttgag atcgccggcg gcaaggtggg ggaagagggt
     1021 gaacgcatct tcgacccggc tgtggtgaag gtacgtttga accgcctcct cgtcgatcgg
     1081 tttcgtcggc gaatcgtgct gccagaagag ggcgagctgg ttgcacaccg tcctgagctc
     1141 ctgggtgttg tcctccgtca tggcgatcag gagctccacg gcttcggacg tgatcgtaag
     1201 gccgagcctg cggaagtggg ttctgatcca cgcggatttc tcaccttcca ggagatcata
     1261 gaagattcgg gttcgttcct tggggatcgc atccatgatc ttcttcgtga tcgaggtttc
     1321 gctcgatgca agaacgaccg tagcgctcgg gcttggattg gccaagtatc cggccagctc
     1381 attgaggaat gcagcgccgg cgctttccag ttggctgatg agaatcaggc gatggtcgct
     1441 gaagagcgag tcgttggcaa gtgaagcgaa cagctctccc tgttccccgt caaacggata
     1501 gaagcgctcg aactccaagc cgtcgccgta cttcgccgcc agctcgcttc gaattcgttt
     1561 cacctcgtcc gccttcgccc cggcttcggg gccgagaagg agatagctgc tcatgaaaag
     1621 tccctgatga gaagttggag aatcccgtgg acccggcatg agcgggtgaa gatcgggccc
     1681 atcgcggagt tggccggcct gagttcaatc atgtcttcac cgggataata gccattcagc
     1741 gtgattcctt cctcgccatc ctccaccgtc gccg
//
LOCUS       DAOPUZ010000300         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_74051_length_1770_cov_3.099708, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000300 DAOPUZ010000000
VERSION     DAOPUZ010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74051_length_1770_cov_3.099708"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKN79_05340"
     CDS             <1..878
                     /locus_tag="PKN79_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ95221.1"
                     /translation="MVFQNYAIFPHLTVRKNVEFGLKSRKYSREEITKQAEKYMRLMQ
                     IQQFAERMPAQLSGGQQQRVALARALCIEPSVLLMDEPLSNLDAKLRVEMRSVIKDTQ
                     RDVGITTIYVTHDQEEAMAISDRIAVMDEGVIQHIGTPKDIYQRPANLFVATFIGRTN
                     IVDAELVVKEGKTFLKVGSFDVEMTNVEKKHAKNQKVIVSIRPEEYKILPKEEKGIKA
                     EVKESVFLGLNTHYFVRTERGNDVEVVQESTIDSIIPPGETISLGLNVSKINLFTSDG
                     SANILKGVKNDLANV"
     gene            891..>1770
                     /locus_tag="PKN79_05345"
     CDS             891..>1770
                     /locus_tag="PKN79_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ95222.1"
                     /translation="MFAKKRFDVWSGVTLGFVILFGTFLVYPMFGILRQSVLNEGGEF
                     TLQHFVKFFSQKYYSMTIWNSLKVTISITLVTLLIGIPFSYFYTFYHIKGSKVIFVVS
                     ILCAMSAPFIGAYAWILLLGRGGVITQFLKNQLNINIGSIYGFKGILIVQSLKLYPLV
                     FLYMNGAFRNIDSYLLEASASLGCVGIKRFFRVVLNLSMPTILAASLLVFMQAFADFG
                     TPILIGEGYRTFPVEIYNQYLGEVGTDYNFASAISVIAIIMTALVFFLQKYATSKYNF
                     SMNATRPVEKKKAKGIR"
BASE COUNT          438 a          455 c          464 g          413 t
ORIGIN      
        1 gcatggtgtt ccagaactat gcgatcttcc cccaccttac cgtccgaaaa aacgttgaat
       61 tcgggttgaa aagccgaaag tattcgagag aggagattac aaaacaggcg gaaaagtata
      121 tgcgcctgat gcagatccaa cagtttgccg aacggatgcc cgctcagctg tccggaggac
      181 agcaacagcg ggttgccctt gcccgagccc tttgcatcga gccttccgtc ctgttgatgg
      241 atgagcctct ttccaacttg gatgcaaagc ttcgggttga gatgcggtcg gtcatcaagg
      301 acacgcagcg ggacgtgggg atcaccacaa tctacgtgac gcacgaccag gaggaggcga
      361 tggcgatcag cgaccgcatc gcagtcatgg atgagggcgt gatacagcac atcggcactc
      421 cgaaggatat ctatcaacgc ccggccaatc tgtttgttgc aaccttcatc ggtcgaacga
      481 acatcgtgga tgccgaactg gtcgtcaaag agggaaagac gtttctcaag gtcggctcct
      541 tcgatgtcga gatgaccaac gttgaaaaga agcatgcgaa gaaccagaag gtgatcgttt
      601 ccatccgacc cgaagagtac aagatcctcc cgaaagaaga aaagggaatc aaggccgagg
      661 tgaaggagag cgtcttcctt gggctcaata cccactactt cgtccgaacc gaacgaggta
      721 acgatgtcga agtggttcag gaatcgacga tcgattccat cattcccccg ggcgagacga
      781 tcagtctcgg attgaatgtt tcaaaaatca acctgttcac ttctgacggg tcggccaaca
      841 tcctcaaggg tgtgaaaaac gatttggcca acgtgtaggg gagggaagag atgtttgcga
      901 aaaaacgttt cgatgtatgg agcggagtga cgctggggtt cgtcattctc ttcggaactt
      961 tcctcgtcta cccgatgttc ggcatccttc gccaatcggt tttgaatgag ggcggggagt
     1021 tcacacttca acactttgtg aagttcttct cgcagaagta ctattccatg acgatttgga
     1081 acagcctcaa agtcaccatt tcgataactc tcgtaactct cctgatcggc atcccgttct
     1141 catacttcta taccttctac catatcaaag ggtcgaaggt catcttcgtc gtcagcatcc
     1201 tctgtgcgat gagcgctccg ttcatcgggg catacgcctg gattctgctg ttgggccggg
     1261 gcggggtgat cacccagttt ttgaaaaacc agctcaacat caacatcggc tcgatctacg
     1321 ggttcaaggg gatcctgatc gttcaatcgc tcaagctcta tccgttggtg ttcctctata
     1381 tgaacggggc gttcaggaat atcgacagct atctgcttga agcctccgcg agcctcggat
     1441 gcgtcggcat caagcgcttc ttcagggtgg tgctcaacct ttcgatgccg acaatccttg
     1501 cagcttcctt gctggtgttc atgcaggcgt ttgccgactt cggaacgccg attctcatcg
     1561 gagaagggta cagaaccttc ccggtcgaaa tctacaacca gtatctcgga gaagtcggaa
     1621 ccgattacaa cttcgcttca gcaatcagcg tcatcgcgat catcatgacc gctttggtgt
     1681 ttttcctcca gaaatatgca acgagcaagt acaacttttc gatgaatgca acccggccgg
     1741 ttgagaaaaa gaaggcaaag ggcattcgag
//
LOCUS       DAOPUZ010000301         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_75066_length_1755_cov_2.847059, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000301 DAOPUZ010000000
VERSION     DAOPUZ010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75066_length_1755_cov_2.847059"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1038)
                     /gene="tsaD"
                     /locus_tag="PKN79_05350"
     CDS             complement(19..1038)
                     /gene="tsaD"
                     /locus_tag="PKN79_05350"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNZ95223.1"
                     /translation="MNVLGIETSCDECAASVVVDGKTILSNVIATQTEEHRVYYGVVP
                     ELASRIHTEWIAPTVQAAIDEAGVEIDGVAVTNRPGLLGSLLVGVSFAKGYAAAKGVP
                     LIGIDHIRAHLYAPQIEHELDYPYLGVLVSGGHTVICKVTGYDDLEVLGTTIDDAIGE
                     AFDKVAKHYDFGYPGGIAIDRLAQKGNPLAFRFPGPKIREKTNPYDISYSGLKSAVIN
                     HLDKYWDGKSEKSSANIAASFQRTAINMLIKRIRAALADTGLKSISVGGGVAANSYLR
                     SELKKFGSGGYTVAFPSMELCTDNAAMIAALGYHYLKDGIRDRYDMKASARVSAFKKQ
                     YTPGG"
     gene            complement(1035..1244)
                     /locus_tag="PKN79_05355"
     CDS             complement(1035..1244)
                     /locus_tag="PKN79_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95224.1"
                     /translation="MKKRMVFTPAAPREVIEILVSHPARFRPIVGQSWDPNVADFLIE
                     MYPEAIEQGFALEEIALMVAEDEEA"
     gene            1344..1415
                     /locus_tag="PKN79_05360"
     tRNA            1344..1415
                     /locus_tag="PKN79_05360"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1376..1378,aa:Thr,seq:cgt)
     gene            complement(1499..>1755)
                     /locus_tag="PKN79_05365"
     CDS             complement(1499..>1755)
                     /locus_tag="PKN79_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95225.1"
                     /translation="DKGFYDYDPFETMDFDGVGKLVRMASDLGRTANSDLVLGICGEH
                     GGDPKTIEFFNEVGLDYVSCSPFRVPIARLAAAQAVIKQK"
BASE COUNT          377 a          504 c          456 g          418 t
ORIGIN      
        1 ccagatcgat gctcacactc agcctcctgg ggtgtactgt ttcttaaacg cactgacacg
       61 tgcacttgct ttcatatcat agcggtcccg gattccatcc ttgagatagt ggtatcccag
      121 agcggcaatc atcgccgcat tgtcggtaca taactccatg gagggaaaag cgaccgtata
      181 tccgccgctc ccaaacttct tcagttcgct gcgaaggtag ctgtttgcgg ccactccccc
      241 accgacgctg atgctcttca gcccggtatc cgccaaggcg gctcgaatcc gcttgatcag
      301 catattgatt gctgttcgtt gaaaggaggc ggcgatgttc gcactcgatt tctcgctctt
      361 gccatcccaa tatttgtcca gatggtttat cacggcgctc ttcaatccgg agtatgagat
      421 atcgtagggg ttcgtctttt cgcggatctt cggcccgggg aagcggaagg caaggggatt
      481 gcctttttga gcgagccggt cgatcgctat ccctcccgga tacccgaagt catagtgttt
      541 ggcgaccttg tcaaatgcct cgccgatcgc atcatcgatc gtggtcccca acacttccaa
      601 gtcatcatag ccggtcacct tgcagatcac cgtatgtccg ccggaaacaa gaactcccag
      661 atagggatag tcaagctcgt gttcgatctg aggggcatag aggtgtgccc ggatatgatc
      721 gattccgatc aggggaaccc ctttggctgc ggcgtaccct ttggcgaagc tcacacccac
      781 cagaagcgag ccgagaagac cgggccggtt ggtaaccgct acaccatcga tttcgactcc
      841 ggcttcatcg atggcggctt gaacggtcgg agcgatccat tcggtatgaa ttcgagaggc
      901 cagctcgggc acgactccat agtagacccg gtgctcctcg gtctgcgtcg caatgacatt
      961 gctgagaatc gtttttccat cgacaacgac gctcgctgcg cactcatcac aggaggtctc
     1021 gattccaagg acgttcatgc ctcttcatcc tcggcgacca tcagcgcgat ctcctccaga
     1081 gcgaaaccct gttcaatcgc ttccggatac atctcaatga gaaaatcagc cacattcgga
     1141 tcccagcttt gaccgacgat cggtctgaaa cgggctggat ggctgacgag tatttcaatc
     1201 acttcacggg gtgctgcggg ggtaaatacc atacgttttt tcaatcaatt tcctcagagc
     1261 cagcctactt caaacaccaa tactggtcaa gttatcccat cccttgcata aaatcactcg
     1321 gtacggtatt atgccctcgt tatgccaaaa tagctcaggg gtagagcggc tcactcgtaa
     1381 tgagcaggtc aagggttcga ttcccttttt tggctgaagc ggctctcagc cgctttttct
     1441 ttctttatcc tgttgcattg caagaaaaag aatcccgggc cgaagcccgg gaagattgct
     1501 acttctgctt gatcaccgct tgggcggcag ccaggcgggc gatgggaacg cggaaggggg
     1561 aacaggagac gtagtcgagt ccgacttcgt tgaagaactc gatcgtcttc ggatcgccgc
     1621 cgtgctcgcc gcagataccc agcacgagat cggaattcgc cgttcggccg aggtcgctgg
     1681 ccattcgaac cagcttgccc actccgtcga aatccatcgt ctcaaacggg tcgtagtcgt
     1741 agaagccctt gtcgg
//
LOCUS       DAOPUZ010000302         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_75163_length_1753_cov_3.485866, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000302 DAOPUZ010000000
VERSION     DAOPUZ010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75163_length_1753_cov_3.485866"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1753
                     /locus_tag="PKN79_05370"
     CDS             <1..>1753
                     /locus_tag="PKN79_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNZ95226.1"
                     /translation="PFSAKHFVQALPASVKRIAVLDRTKEPGSLGEPLYLDVITALNQ
                     MNRTGIKVIGGRYGLSSKDFSPTHAKAIFDHLAGPAFSNFTVGIKDDVTNRSIEIKEQ
                     INVIPKDVVSCMFWGLGSDGTVGANKNSVKIIGDNTDLNAQAYFSYDSKKSGGITISH
                     LRFGKSSLEMPWLVDHADFVACHNPAYIGRYDMLAPLRKGGVFLLNSQIPSDEVFDHL
                     TREMQEQIIEKELRFFNINALEIAEGVGLGSRINTVMQAAFFKISEVLPEEEAIALIK
                     EYVKKTFLRKGEDVVKMNWAAIDGASDALHRVEIPKTITKSYEPAPLIPEDADRFSKK
                     IIEPIMHLKGNNIPVSEMSFDGTLPTGTAKLEKRGVAPFVPKWIAENCIQCNQCVQSC
                     PHAAIRAKQIEPERLKDAPETFKVLKSISKNDRDLQFKIQVYTEDCQGCGVCIETCPS
                     KVKSLEFSPIQVERDAGEIANAEFFEQLPYDIIDGSALNSIKGLQFKQPLFEFSGACA
                     GCGETPYVKMVSQICGDRMIVANATGCSSIYSGTFPTTPYTVRQSDGQGPAWGNSLFE
                     DNAEYGLGMRLAVDANRE"
BASE COUNT          386 a          535 c          485 g          347 t
ORIGIN      
        1 cccttcagcg ccaagcactt cgttcaagcg cttcccgctt ccgtaaaacg cattgccgtt
       61 cttgaccgga ccaaagagcc gggcagtctc ggcgagccgc tttatctgga cgtcatcacc
      121 gccctcaacc agatgaaccg aaccggaatc aaggtcatcg gcggccgcta cggtctttcc
      181 agcaaggact tctccccgac ccacgcgaag gcgatcttcg atcacctcgc cggtccggcg
      241 ttcagcaact tcaccgttgg catcaaggat gacgtcacca accgctccat cgagatcaag
      301 gagcagatca acgtcattcc gaaggatgtc gtatcgtgca tgttctgggg gctcggttcg
      361 gacggcaccg tcggagcgaa caagaactcc gtcaagatca tcggcgacaa caccgatctg
      421 aacgctcagg cgtacttctc ctacgactcc aagaagagcg gcggcatcac gatcagccac
      481 cttcgcttcg gcaagagctc gcttgagatg ccgtggctgg tcgaccacgc cgatttcgtc
      541 gcctgtcaca atccggcgta tatcggccgc tacgacatgc tcgctccgct tcgcaagggt
      601 ggcgtgttcc tcttgaacag tcagattcct tccgatgagg tcttcgacca tctgacccgc
      661 gagatgcagg agcagatcat cgaaaaagag cttcggtttt tcaacatcaa cgcactcgaa
      721 attgctgagg gcgtcggtct cggatcccgg atcaacacgg tgatgcaggc ggcgttcttc
      781 aagatcagcg aagtccttcc cgaagaggag gcgatcgccc tgatcaagga gtacgtgaag
      841 aagacgttcc tccgcaaggg tgaggatgtc gtcaagatga actgggcggc gatcgatggc
      901 gcctccgatg cgctccatcg ggtcgagatc cccaagacaa tcaccaagag ctatgagccg
      961 gctcccctga tccccgagga tgccgaccgc ttctcgaaga agatcatcga gccgatcatg
     1021 cacctcaagg gcaataacat tccggtttcc gagatgtcct tcgacggaac gcttcccacc
     1081 ggtacggcca agcttgagaa gcgcggcgtc gcgccgttcg ttcccaagtg gatcgccgag
     1141 aactgcatcc agtgcaacca gtgcgtgcaa agctgtccgc acgctgcgat ccgggcgaag
     1201 cagatcgagc ctgagcgcct gaaggatgct cccgagacgt tcaaggttct gaagtcgatc
     1261 tccaaaaacg atcgggatct tcagttcaag attcaggtgt acaccgaaga ctgtcagggt
     1321 tgtggcgttt gtatcgaaac ctgtccttcg aaggtcaaga gcctcgagtt cagtccgatt
     1381 caggtcgagc gcgatgccgg cgagattgcc aatgcggagt tcttcgagca gctcccgtac
     1441 gacatcatcg acggaagtgc cctcaactcg atcaaggggc ttcagttcaa gcagccgctc
     1501 tttgagttca gcggagcctg cgccggctgc ggcgagactc cctatgtaaa gatggtctcc
     1561 cagatctgtg gcgatcggat gatcgtcgcc aacgcaacag gttgttcctc gatctacagc
     1621 ggaacgttcc cgaccacccc gtacaccgtt cgccaaagcg acggtcaggg tcctgcctgg
     1681 ggcaacagcc tcttcgagga caacgccgag tatggtcttg gaatgcgcct cgccgtcgat
     1741 gcgaaccgcg aac
//
LOCUS       DAOPUZ010000303         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_75250_length_1752_cov_2.922216, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000303 DAOPUZ010000000
VERSION     DAOPUZ010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75250_length_1752_cov_2.922216"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PKN79_05375"
     CDS             complement(<1..833)
                     /locus_tag="PKN79_05375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ95227.1"
                     /translation="MFDKRKTTLGGQPFIVRPYQSQAADALLGDLGRGRGFGTIVLPC
                     GSGKTIVGLEIMSRLKTSTLIVTTNVVAVHQWMREILDKTTLEREDVGEYTGNLKERK
                     PITVCTYQVLTYRKSKTAPFAHMDLLTKGRWGLIIYDEVHMLPAPVFKVTAELQATFR
                     VGFTATLVREDGREDEVFSLVGPKRYDVPWIELQQQGFIAQAYCHEIRLDLPKELELE
                     YALATKRQKYRIASENPLKIEVVRSLIERHSDDFILIIGQYIDQLEEVARDFLERYYK
                     EA"
     gene            complement(1017..1271)
                     /locus_tag="PKN79_05380"
                     /pseudo
     CDS             complement(1017..1271)
                     /locus_tag="PKN79_05380"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
     gene            complement(1171..>1752)
                     /locus_tag="PKN79_05385"
     CDS             complement(1171..>1752)
                     /locus_tag="PKN79_05385"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNZ95228.1"
                     /translation="GLGEPVFGKLDALLAHAILSIGAVKGIQFGDGFALSAMRGSHAN
                     DQRTKDGFLSNHSGGILGGISSGQEIVFQAAIKPTSSIGIGQRTVDKEGNDVTLTVTG
                     RHDPCICGRAVVVIEAMTAITSSTSITRRSGKMHERYSPDRPKRPQPPSGCPPPGQRS
                     VSERSDPFLRIGEISRAYAYLSNQRDQPVERLR"
BASE COUNT          357 a          499 c          482 g          414 t
ORIGIN      
        1 gcttctttgt aatacctctc cagaaaatcc ctggccacct cctcaagctg gtcgatgtac
       61 tggccgatga tcaggataaa gtcatcgctg tgccgttcga tgagcgagcg gaccacctcg
      121 atcttcaacg ggttttcact ggcgattcga tatttttgac gcttggtggc caaggcatat
      181 tcaagctcca gctctttcgg cagatccagg cgaatctcgt ggcagtacgc ctgggcgatg
      241 aacccctgct gctgaagttc gatccacgga acgtcgtatc gcttcggtcc gaccaagctg
      301 aagacttcat cctcccgccc gtcttcccgg accaaggtgg cggtgaaacc gacccggaac
      361 gtcgcctgca attccgccgt cactttgaaa acgggcgcgg ggagcatatg gacttcatcg
      421 tagatgatga gaccccaccg tcctttggtc aggagatcca tgtgagcaaa cggggcggtt
      481 ttgctcttcc ggtaggtaag gacctgatag gtgcacaccg ttatcggttt ccgttctttc
      541 agattgccgg tatattcacc gacatcttcg cgctcgagcg tggtcttgtc gaggatttcc
      601 ctcatccact ggtggaccgc caccacgttg gtggttacga tcagggtgct ggttttcaat
      661 cggctcatga tttccaagcc gacgatcgtt tttcccgatc cgcaaggcag gacgatcgta
      721 ccgaagccgc ggccccggcc aagatctccc aaaagggcat cagccgcttg gctctggtac
      781 ggtcggacga tgaacggctg tccgccaagg gtcgtcttac gtttgtcaaa catgagagga
      841 ggacctttct caagaggaat tcgatcatcc accgggtagc cgagcttgat gagacgaagc
      901 ttgagctcgc cgcgcaggta ccgttcaacg aggaaagagt cctcgtcctt gacgacgaga
      961 atcttcatca gctccttgcg gtgtttgatt tccagtcgga ttttttcaat ggaaatcgtt
     1021 aaaagataat aggcttcatc agtgctttcg gtcaggacca gagaccccca tcggccggcg
     1081 atgtcccgaa cgaaaaagag tacgctttcc ggcggcggaa actttgacca ctttttaatc
     1141 atttcaacga tcgcttcccc gtcaagcccc tcaccggagg cgttccacag gctgatctcg
     1201 ctgattcgat aggtatgcat atgctcggga gatttcacca attcgcaaaa acggatcaga
     1261 tctttccgac acgcttcgct gtccgggtgg tggacatcca gaaggaggct gcggtcgctt
     1321 tggacgatca ggggagtatc tttcatgcat ctttcccgaa cgccgtgtaa tagaggtcga
     1381 ggaggtgatg gcggtcatcg cttcgatgac gacgaccgct cgaccgcaga tgcacgggtc
     1441 gtggcggccg gtcacggtga gcgtcacatc gtttccctcc ttgtcaaccg tccgctgccc
     1501 gatgccgatc gagctggtcg gcttgatcgc ggcttggaaa acgatctcct gaccgctgga
     1561 gattccgccc aggatgcctc cgctgtggtt gctcaaaaac ccgtctttgg tccgctgatc
     1621 attggcatgg ctgcctcgca tcgcggacaa ggcgaacccg tcgccgaatt gaatcccttt
     1681 taccgctccg attgaaagga tcgcgtgggc gaggagcgcg tcgagttttc cgaacaccgg
     1741 ttccccgaga cc
//
LOCUS       DAOPUZ010000304         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_75306_length_1751_cov_3.185142, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000304 DAOPUZ010000000
VERSION     DAOPUZ010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75306_length_1751_cov_3.185142"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PKN79_05390"
     CDS             <1..351
                     /locus_tag="PKN79_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95229.1"
                     /translation="ILGLYLPRFTSLLYGLLFIALALVGTLLFVLMVGTNMNVTYWNL
                     NILFINPLLFCVAFRPKKQGKVLLFGLGSMIVLLALELLNAPFHQDSTLLIALLLPIY
                     LSRAAFQGRTEIKQ"
     gene            complement(310..870)
                     /locus_tag="PKN79_05395"
     CDS             complement(310..870)
                     /locus_tag="PKN79_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95230.1"
                     /translation="MSKAKRFFPDLKSLIVSTVVILLLLIFAVVVTDHNNVRRLHRYL
                     WEAEAAKEACYSLIEQRLGYAKALVRIIDNQVDTDRVEEVIGRWDAAASVDEASVLYK
                     TLDDELALLQRKAVEHESYRAWSPYFDRMYLLELELVDISAHYQERAEFFNAQKGGFP
                     ARLAAKRHNLEDLLLFDFGSSLKGRP"
     gene            complement(857..>1751)
                     /gene="arcC"
                     /locus_tag="PKN79_05400"
     CDS             complement(857..>1751)
                     /gene="arcC"
                     /locus_tag="PKN79_05400"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNZ95231.1"
                     /translation="PKHVTVQSQYEAARKTASHIARLIQAGHRVLIAHGNGPQVGYVL
                     LRSDIAKDVLHQVPLDSCVADTQGAIGYQLQMALHNELTELGVTCPVVTVVTQVEVDA
                     NDPSFYNPTKPIGSFLTRNEAEERKTKDGWNVVEDAGRGYRRVVPSPKPKRIVELDSI
                     RSLLENGSIVIAAGGGGIPVIRDEDGMLRGKEAVIDKDLAAALLAKELNADLFVISTA
                     VEKVFLNWGKPTQKSLDLITSTEAKQYIKEGHFAPGSMLPKVEAIVDFVEVTNNTGLI
                     TDPANIHGALYRDKGTKIVKG"
BASE COUNT          358 a          493 c          454 g          446 t
ORIGIN      
        1 atcctcggct tgtaccttcc ccgcttcacc tcgctgctct atggactact ctttatcgcc
       61 cttgctctgg tgggtaccct gctcttcgtg ttgatggttg gaaccaacat gaacgtgacc
      121 tattggaact tgaacatcct gttcatcaac cctcttctct tctgcgtcgc gttccgtccc
      181 aaaaaacagg gcaaggttct cctcttcggt ctcgggtcga tgatcgtcct gcttgcgttg
      241 gagcttttaa acgctccgtt ccatcaggat tcgacccttc tcatcgcgct tctgctgccg
      301 atctatcttt cacgggcggc ctttcaggga agaaccgaaa tcaaacagta agagatcctc
      361 aagattgtga cgttttgcgg ccaagcgtgc cggaaatccg cccttctgcg cattaaaaaa
      421 ttcagcgcgt tcctgatagt gggccgagat gtcgaccagc tccagctcca acagatacat
      481 ccggtcaaag tacggcgacc aggcgcggta tgattcatgt tcgaccgcct ttcgttgaag
      541 aagggcaagc tcatcgtcga gggttttgta aagaacgctt gcttcatcta cggaagcagc
      601 tgcatcccat cgaccgatca cttcttccac tctgtcggta tccacttgat tatcaatgat
      661 gcgaacgagc gccttggcgt agccgaggcg ctgttcgatc agtgaatagc acgcttcttt
      721 agccgcttcc gcttcccaca gatagcgatg aaggcgccgg acgttgttgt gatcggtaac
      781 aacgacggca aaaatcagaa gaagaaggat gacgaccgtt gagacgatga gggatttcag
      841 atcgggaaaa aatcttttag cctttgacaa tcttcgtccc tttgtcccga tagagcgctc
      901 cgtggatgtt tgccgggtcg gtgatcaagc ccgtgttgtt cgtaacttcg acgaaatcaa
      961 cgattgcctc gactttcgga agcatcgatc cgggggcgaa gtgtccttct ttgatatact
     1021 gtttcgcctc ggtggaggtt atcaggtcca agctcttttg cgtgggcttg ccccagttga
     1081 gaaacacttt ttccaccgcc gtggagatga caaacagatc ggcgttcagt tccttggcca
     1141 gcagggctgc tgcaagatcc ttgtcgatga ccgcttcctt ccctcggagc attccgtctt
     1201 catccctgat caccggaatc cctccacccc ccgcagcgat gacgatcgag ccgttttcca
     1261 aaagggaacg gatcgaatcc agctcgacga tccgtttggg cttgggcgaa ggaaccaccc
     1321 ggcgatagcc gcgtccggca tcttcgacta cgttccatcc atccttggtt ttccgctctt
     1381 cagcttcatt tcgggtcagg aaggaaccga tcggtttggt gggattgtaa aaggacggat
     1441 cattcgcatc gacctcgacc tgcgttacca ccgtcacgac cgggcaggtc actcccagct
     1501 cggtaagctc attgtgcaga gccatttgaa gctgatagcc gatcgctccc tgcgtgtccg
     1561 ccacgcagct gtcaagcggc acctggtgca gaacatcttt ggcgatatcg cttcgaagca
     1621 ggacgtatcc gacttgagga ccgttgccgt gggcgatgag cacccggtga ccggcttgga
     1681 tcagacgggc gatatggctg gcggtttttc gggccgcttc atattggctt tgaaccgtaa
     1741 catgctttgg a
//
LOCUS       DAOPUZ010000305         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_75366_length_1750_cov_3.401770, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000305 DAOPUZ010000000
VERSION     DAOPUZ010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75366_length_1750_cov_3.401770"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PKN79_05405"
     CDS             <1..846
                     /locus_tag="PKN79_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ95232.1"
                     /translation="LRKGRIPLFIIVLFISALLILLPVVVLFYTSLVPYVMVPGAKAF
                     AAMSWDNWKTVIKDPISVRSLKNSLFLGIAGATVGSFLSVLVAYVIVKVKTKAAGVLE
                     SISFLSFSFPGIVIGIGFMWYFVRTPLYATIWALFFGYIATYLPYGIRPMTSAFIQIH
                     GSLEESSLVCGASFTTTLRRIVLPLLVPGFVSAWILLATMFFRELSISVVLSRPGTET
                     LAVQILRFSEDGQWGKLSALGLIMIFVSTAMVLAVNALGAYFKPGAVREKKRGGVKAG
                     LKAAV"
     gene            complement(851..>1750)
                     /locus_tag="PKN79_05410"
     CDS             complement(851..>1750)
                     /locus_tag="PKN79_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNZ95233.1"
                     /translation="AFPIQDALPLSSYWMICLLGIILGGAGSLYNLVLLKTQKLYKKV
                     SNTRIRVMIPFFLAGLLSLVFPAVLGGGHAAMEYLNLSSGLGFLTVLFLVKFIFSMIS
                     FGSGAPGGIFFPLLILGAIIGALFGTFSILYLGLDSAYMNNFIILAMTGFFSAIVRAP
                     LTGIVLLLEMTGSFTSMLPLALVAIIASTTADLLKSTPIYHSLLENQRKEAGSANLPD
                     VGQTTTVEMVVHHGSPASDNRVKDLDLPDNCLLIGIRRDGKEIIPRGGTLIRANDYII
                     FLINLHEEADHRDTLTKLFSVHE"
BASE COUNT          395 a          440 c          495 g          420 t
ORIGIN      
        1 ctgagaaagg gaaggattcc gctgttcatc atcgttctgt tcatcagcgc cctgttgatt
       61 ctgcttcccg tcgtagttct gttctacacc tcgcttgttc cgtacgtcat ggtacccggg
      121 gcaaaggcgt ttgcagcgat gagttgggac aactggaaaa ccgtcatcaa ggatccgatc
      181 tcggtgagat cgctcaagaa cagcctcttc ttgggcatag cgggtgctac cgtcggttcc
      241 ttcctctccg ttctggtggc gtatgtgatc gtgaaagtga agacgaaggc cgcaggcgtc
      301 ttggagtcga tcagcttcct ttccttctca tttccgggaa tcgtcatcgg tatcggcttc
      361 atgtggtatt tcgtgcggac tccgctctac gccacgatct gggcgctctt cttcggatac
      421 atcgcaacct acctgccgta tgggattcgg ccgatgacca gcgccttcat ccagatccat
      481 ggcagcttgg aagagtcctc gctggtctgc ggggcttcct tcacgacgac gctgcgccgc
      541 atcgtcctcc cgcttcttgt tccggggttt gtttccgctt ggattctgct ggctacgatg
      601 tttttccggg agctttccat ctcggtcgtt ctttcccgtc cgggaaccga gacgttggcg
      661 gtgcagatcc tccgcttcag cgaggatggc cagtggggca agctcagcgc ccttggactg
      721 atcatgattt tcgtctccac tgcgatggtt ctcgcggtga acgccctcgg cgcctacttc
      781 aaacccgggg cggtgaggga gaaaaagagg ggaggagtca aggcagggct caaagcggcg
      841 gtataaggat ttattcatgt accgagaaca gcttggttaa tgtatctcgg tggtcggctt
      901 cctcgtgaag gtttatcaga aaaataatgt aatcgttggc tcggatcagg gtgcctccgc
      961 gtggaatgat ctctttgcca tccctgcgga ttccgatcag gaggcagttg tccggaagat
     1021 caagatcctt tacccggtta tcggatgcag gcgagccatg atgcacaacc atttcaacgg
     1081 tggtcgtttg ccccacgtcg ggaaggttgg cggaaccggc ttccttgcgc tggttttcaa
     1141 gaagcgaatg ataaataggt gtgcttttta aaagatctgc ggttgtggag gcgatgatgg
     1201 cgaccaacgc aagaggaagc atgcttgtaa acgatccggt catttccaac aggagaacga
     1261 taccggtaag cggcgcgcgc acgatcgctg aaaagaatcc cgtcatggcc aaaatgatga
     1321 aattgttcat atatgccgaa tcaagcccca gatacaggat ggagaatgtt ccgaagaggg
     1381 ctccgatgat tgcaccgagg atcagcaagg ggaagaagat gccgcccggg gctccggatc
     1441 cgaaacttat catcgaaaag atgaatttga caagaaaaag cacagtgaga aaccccagac
     1501 cgcttgacag gttcaggtat tccatcgcgg catggcctcc tcctaaaaca gcggggaata
     1561 cgaggctgag aagacccgca aggaagaacg ggatcatcac ccgaattcgt gtattggata
     1621 cttttttata cagcttttgt gttttcagca gaacgaggtt atacaatgaa ccggcacctc
     1681 ccaagatgat gccgagcagg cagatcatcc aataagagct caagggaaga gcgtcctgaa
     1741 tgggaaaagc
//
LOCUS       DAOPUZ010000306         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_75800_length_1743_cov_2.839455, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000306 DAOPUZ010000000
VERSION     DAOPUZ010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75800_length_1743_cov_2.839455"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..>1743
                     /locus_tag="PKN79_05415"
     CDS             105..>1743
                     /locus_tag="PKN79_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ95234.1"
                     /translation="MFLKSLELYGFKSFADRVKLQFSEGITSLLGPNGCGKSNIVDAI
                     KWVLGEQSTKTLRASKMEDVIFNGTDLRKPLQVAEVILVLSNENGDLPIDEPEVEIKR
                     RIFRTGESEYYINRNRARLMDIRDLFFDTGVGKSAYSILEQGKIDQILSSRPEDRRYI
                     FEEAAGISRYKVQSLEAKRKLERTDENIAQVETILREVKRNYDTKKGQAEKAKNYREL
                     RKEQFTLEVDIQLSTLVSYSKLREVRLEQERTFSRHYQAKRAELETFDESLANLREEL
                     RTLGASRIAIQTELQRLDEATKGRGEKLDLLTVRYRDFLNQQESAKSRSEGIQGQINR
                     DKAHIAERTEHITQIDEQIRQLEGELEGFLASLGKNRKTIEDHINQINKLEKSNARLE
                     EETDALSLEVKELSDAIVIQLEEQLRSSGYTTKARNEARSILLSELEHIKQSILKEQE
                     FLAKLKAADLSVETLFKRQHEFFSTLGDSLASLSGHFTAYEGTQASFLDELIAPEGTI
                     AKKQSLDLAIAQKRKEIIRNRERVSHLREENLHLQSQM"
BASE COUNT          483 a          459 c          470 g          331 t
ORIGIN      
        1 ccagcttcat caaccccaca gatcaacgtc ataccctatt acctccgccg cctgatatgc
       61 tataatcgct ctcagatatc gtcaagataa gaagagggtg tgcgttgttt cttaaatcac
      121 ttgaactcta tggattcaaa tcatttgccg atcgggtgaa gctccaattc tccgagggga
      181 tcaccagtct cctgggaccg aacgggtgtg gcaaatccaa tatcgtcgat gcgatcaaat
      241 gggttctcgg cgagcaatcc accaagacgc tccgggcatc gaagatggaa gatgtgatct
      301 tcaatggaac cgacctgcgc aaaccccttc aggtcgccga agtgattctc gtcctctcca
      361 acgagaacgg cgatctgccg atcgacgagc cggaggtcga gatcaagcgg cggatcttcc
      421 gcaccgggga aagcgagtac tatatcaacc gcaatcgcgc ccggttgatg gacatccgcg
      481 atctcttctt cgataccggg gtcggcaaga gcgcctactc gattctggag cagggaaaaa
      541 tcgaccagat cctttcctcc cggccggagg accggcgcta catcttcgag gaagcggcgg
      601 gaatcagccg atacaaggtg caaagtctgg aagcgaagcg caagctggag cgaaccgatg
      661 agaacatcgc tcaagtggaa acgattctca gggaagtgaa gcgcaactac gatacgaaga
      721 aaggacaggc ggaaaaagcg aagaactatc gagagcttcg aaaagagcag ttcactctgg
      781 aagtcgatat ccagctttcc accctagtca gctattcgaa gctgagggag gtccggctcg
      841 aacaggagcg gacgttctcc cggcactatc aggcgaaacg ggcggaactg gaaaccttcg
      901 acgagagcct cgccaatctg cgcgaagagc tgaggacctt gggcgcctcc cgcatcgcta
      961 ttcagaccga gctccagcgc cttgatgagg cgacgaaggg aaggggcgaa aaacttgatc
     1021 tgctgacggt gcggtaccgg gatttcctga atcagcagga aagcgcaaaa agccgcagtg
     1081 aggggattca aggccagatc aatcgcgata aagcgcatat tgccgagcga accgaacata
     1141 tcactcagat cgatgaacag atcaggcaat tggagggaga gttggaaggg tttctggcct
     1201 cccttgggaa aaaccggaag acgatcgaag atcatatcaa tcagatcaac aaactcgaga
     1261 agagcaatgc ccggctcgaa gaagagaccg acgcgctgag ccttgaggtc aaggagctca
     1321 gtgatgcgat cgtcattcaa ctggaagaac aactgcgctc aagcggatat accaccaaag
     1381 cccgaaatga agcccgaagc attctcttgt cggagttgga gcatatcaag caatcgattc
     1441 tcaaagaaca ggagtttctc gccaagctga aagccgccga cctttccgtc gagacactgt
     1501 tcaaacggca acatgaattt ttttccacct taggcgactc attggcttca ctttccggtc
     1561 attttaccgc ctatgaaggg actcaggcat ctttcctgga tgagctcatc gcccccgaag
     1621 ggacgatcgc aaaaaaacaa tcattggatc tggcgatcgc tcagaaacga aaagagatca
     1681 tccgcaaccg ggagcgggtt tcccacctca gggaggagaa ccttcatctc cagagtcaga
     1741 tgg
//
LOCUS       DAOPUZ010000307         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_76169_length_1737_cov_3.441141, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000307 DAOPUZ010000000
VERSION     DAOPUZ010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76169_length_1737_cov_3.441141"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PKN79_05420"
     CDS             complement(<1..470)
                     /locus_tag="PKN79_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNZ95235.1"
                     /translation="MSDNALYVSYNTVHKLVKSLSERLLDSGYDPEVIVAIGSGGFIP
                     ARIMKTFINRPIYAVGISYYGVDNKHRDHPTKIQWIDEVKSQLRGKKVLLIDEVDDSR
                     VTLAYCIGELLKYEPEEIAVLVLHNKRKEKDVEFPSEIKRYFSGMETDDVWIKYP"
     gene            complement(473..1033)
                     /gene="hpt"
                     /locus_tag="PKN79_05425"
     CDS             complement(473..1033)
                     /gene="hpt"
                     /locus_tag="PKN79_05425"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNZ95236.1"
                     /translation="MNNSLSIPPLAKDLVRVLIDAATIERRVDELARQITEDYQNLEG
                     DLILVGVLKGSFIFIADLARKMMKKHVIDFIALSSYDGGTSGNVRLLMDTRENMENKH
                     VLIVEDILDSGNTLDYLTRSFMTRSPRSVKTAVLLDKPERHEVDIKVDYIGFTIPDVW
                     VVGYGLDYNERYRTLPYIAEMKPQER"
     gene            1204..>1737
                     /locus_tag="PKN79_05430"
     CDS             1204..>1737
                     /locus_tag="PKN79_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ95237.1"
                     /translation="MRLTKNQAHKALSIMVRSRRFEELVAQFFHHKEMHGTTHLSIGQ
                     EAAQAGLSLALKEGDWIVPTHRCHGHTIARGSGEEVMFSEMFGSRHGIVKGLGGSMHM
                     TDVQTWNTGSSSVVGSGVNLATGLGFALKQQGSDSLSVAIFGDGASSRGAVHESMNLA
                     SVLELPVLFFCENNGYGM"
BASE COUNT          361 a          453 c          522 g          401 t
ORIGIN      
        1 ggatacttga tccagacatc atcggtttcc attccgctga aataacgttt gatttcggag
       61 gggaactcca cgtccttctc cttgcgcttg ttgtgcaata ccaatacggc gatctcctcg
      121 ggctcatact ttaacagttc accgatgcag tacgccagag tgacccggct gtcatcgact
      181 tcgtcgatca gcaggacttt ttttccccga agctggcttt tcacctcgtc gatccattgg
      241 atcttcgtcg gatggtcgcg gtgcttgttg tctaccccgt agtaggagat gcccaccgca
      301 tagatcggtc ggttgatgaa ggtcttcatg atgcgggcgg ggatgaagcc gccgctgccg
      361 atcgcgacga tcacctcggg gtcatacccc gaatcgagca gccgctcact gagcgatttg
      421 acgagcttgt gaacggtgtt gtaggaaaca tacagagcgt tgtcactcat gcttacctct
      481 cctgaggttt catctcggcg atgtagggaa gggttcggta gcgttcgttg taatcgaggc
      541 cgtagccgac gacccagacg tcgggtatcg tgaagccgat gtaatcgacc ttgatatcga
      601 cctcgtgccg ctccggtttg tcaagcagga cggcggtctt taccgaacgc gggcttcggg
      661 tcatgaagct ccgggtcagg taatcgaggg tgttcccgct gtcgaggatg tcttcgacga
      721 tcaaaacatg cttgttctcc atgttttccc gggtgtccat cagaaggcgg acgttaccgc
      781 tggttccccc gtcgtaggag gaaagggcga tgaaatcgat gacgtgtttt ttcatcatct
      841 tgcgcgccag atcggcgatg aagatgaagg agcccttgag gactccgaca agaatcagat
      901 ctccctccag gttttggtaa tcttcggtga tctggcgggc cagctcatcg actcgacgtt
      961 cgatcgtggc ggcgtcgatc aggacgcgga ccaagtcttt ggctagtggc ggaatggaca
     1021 agctgttgtt cataggaacc cccttgggta cgatgtaagg attatataaa aagaggctgt
     1081 tgtcaacgag agctcataaa acgattcctc aacattgaca ggggaaggat tggcgcgtac
     1141 gatacaaaag accaccaact catgtgggaa aggcgggact gcatatcctg ccggggagca
     1201 cagatgagac tgacaaagaa ccaggcgcac aaagcgcttt cgatcatggt tcgttcgcgc
     1261 cgattcgagg aattggtcgc tcaatttttt caccataaag agatgcatgg gaccacccat
     1321 ctttcaatcg ggcaggaggc cgctcaagcc ggtctttctc tggcgttgaa agagggggat
     1381 tggatcgtcc ccacccatcg ctgccacggc catacgatcg cccggggcag cggcgaagag
     1441 gtgatgttca gcgagatgtt cggctcgcgc catgggatcg tgaagggcct cggcggctcg
     1501 atgcacatga cggatgtcca gacgtggaac accggctcct ccagcgtggt cggaagcggt
     1561 gtgaaccttg cgaccgggtt gggcttcgcc ctcaagcagc agggatccga ttcgctttcc
     1621 gtggcgatct tcggcgacgg ggcgagcagc cggggggcgg tgcacgaatc gatgaacctc
     1681 gcctcggtgc tggagctgcc ggtgctcttt ttctgcgaga acaacggcta cgggatg
//
LOCUS       DAOPUZ010000308         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_77461_length_1719_cov_3.134615, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000308 DAOPUZ010000000
VERSION     DAOPUZ010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77461_length_1719_cov_3.134615"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKN79_05435"
     CDS             <1..299
                     /locus_tag="PKN79_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNZ95238.1"
                     /translation="LIITNCIVMGRVEAFALTNKPLPSLVDGLASGLGYAYVLLLISV
                     VRELMGSGSLWGYRILGSWWTNWAIMIMPPGGFFILAIAIWMIRETVGKEVSHA"
     gene            292..885
                     /locus_tag="PKN79_05440"
     CDS             292..885
                     /locus_tag="PKN79_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNZ95239.1"
                     /translation="MPSHVSAFAVFFASIFTSNILLTNYLGMCSFLAVSKEMKTSVGL
                     GAAVTFVLAVTTPLNWLVYHYLLIPFNLEYLRFIVFIIVIAAFVQLTEMTLERYSEPL
                     YQSLGIFLPLITVNCAILGASLFMVIREYTFVTSLLFGIGSGIGWLLAIVAMSGIRTK
                     LRTANIPPALEGPGISLIIAGFMAMAFMGFSGMIAVS"
     gene            896..>1719
                     /locus_tag="PKN79_05445"
     CDS             896..>1719
                     /locus_tag="PKN79_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNZ95240.1"
                     /translation="MIVSLVISVAVISLISVFLALLMVVADATIGNYGIVKVDINDGT
                     RELEVKGGMPLLSTLTSQGVFIPSACGGRGSCGLCKVQVLSGVGDYLPTELPFISKEE
                     QAQNIRLSCQIKVKQNIAIKIPEELFNVRQYKTTVERIRDLTHDIKEVTLRLAPGETI
                     TPKAGQYIQFVVPEYGDLDESVYRAYSVASPPDDSTRVELEIRLVPKGIATTYVHTIL
                     KEGDEVTINGPYGDFYVRDTDRPMICIAGGSGMAPIKSILLDLKNKGSKRKVTYFFG"
BASE COUNT          343 a          513 c          484 g          379 t
ORIGIN      
        1 gactgatcat caccaactgc atcgtcatgg gccgggtcga agcgttcgcc ctcaccaaca
       61 agcccctgcc ctccctggtg gacggacttg caagcggact cggctacgcc tacgtccttc
      121 tgcttatctc ggtggttcgg gagctgatgg gatccgggag cctctggggt tatcggattc
      181 tcggctcgtg gtggaccaac tgggcgatca tgatcatgcc gccgggcggc ttcttcatcc
      241 tcgcgatcgc gatctggatg atccgcgaga ccgtgggaaa ggaggtgtct catgcctagt
      301 cacgtaagcg cctttgccgt ctttttcgct tccattttca ccagcaacat cctgttgacc
      361 aactacctcg gaatgtgttc cttcctggcg gtttccaagg agatgaagac ctccgtcggt
      421 ctcggagcgg cggttacctt cgtgttggcg gttactacgc cgctgaactg gctggtgtat
      481 cactacctcc tcattccgtt caaccttgag tacctgcgct tcatcgtctt catcatcgtc
      541 atagcggcgt tcgtccagct gacggagatg actttggagc gatacagtga acccctctac
      601 caatcgcttg gaatcttcct gcccctcatc accgtgaact gcgcgatcct cggagcgagc
      661 ctcttcatgg tgatccgcga gtatacattc gtcaccagcc tgcttttcgg catcggaagc
      721 ggaatcggat ggctgctggc gatcgtggcg atgagcggaa tccgaacgaa gcttcgaacc
      781 gccaacatcc cccccgcctt ggagggaccg ggaatctctc tgatcatcgc gggcttcatg
      841 gcgatggcgt tcatgggctt cagcggcatg atcgcggttt cctaaggagg atcgcatgat
      901 tgtttcatta gtcatcagcg tcgcggtcat cagcctcatc agcgtgtttc tggcgctgtt
      961 gatggtggtc gccgatgcga cgatcggaaa ctatggaatc gtgaaggtgg acatcaacga
     1021 cggaaccagg gagcttgagg tgaaaggcgg gatgccgctc ctttccaccc tcacctccca
     1081 aggggtgttc attccttcgg catgcggtgg acggggctcg tgcggactgt gcaaagtgca
     1141 ggtgctctcc ggtgtcggcg attacctgcc gacggaactg ccgttcattt ccaaagaaga
     1201 gcaggctcag aacatccgcc tttcctgtca gatcaaagtc aagcagaaca tcgcgatcaa
     1261 gatccccgag gagctcttca acgtccgcca gtacaagacg accgtcgagc gaatccgcga
     1321 tctcacccac gacatcaaag aggtcaccct ccggctggct cccggggaga ccatcacccc
     1381 caaagcgggc cagtacatcc agttcgtcgt tcccgaatac ggggatctgg atgaaagcgt
     1441 ctaccgcgcc tactcggtcg cctccccgcc ggatgacagc acccgggtcg aattggagat
     1501 tcgtctggtg cccaaaggaa tcgctacaac gtacgtccac accatcctga aggaggggga
     1561 cgaggtgacg atcaacggcc cgtacgggga cttctacgtc cgcgataccg accggccgat
     1621 gatctgcatc gccggaggct cgggaatggc cccgatcaaa tcgattcttc tggatctgaa
     1681 gaacaaaggc agcaagcgga aggtaaccta cttcttcgg
//
LOCUS       DAOPUZ010000309         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_77727_length_1715_cov_3.325301, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000309 DAOPUZ010000000
VERSION     DAOPUZ010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77727_length_1715_cov_3.325301"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="PKN79_05450"
     CDS             complement(<1..1385)
                     /locus_tag="PKN79_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNZ95241.1"
                     /translation="MNGIWKLSPRDRHAIAPHCPSFLETPFIDCTLPGDVHSALLKNG
                     LIPDPFVGTNELEIQWVGRHDWVLEKPFTLAEEDFACGTPFITFTMADTMITLFINDR
                     QVGTMYNQFRRWRFEIGEYLQVGENTIKLIFESAENAAIRESRQIPYSEYPVHAKHRN
                     LIRKTQCHSGRDWGPCIMTFGIYEPVKIEFVDEGFIDSVKFTTVETGASSFDVTVEVE
                     YAPLEAIKALDFTATVAGSSQTVTHAFGPGRSNIASLTFAVSDVRRWHPNGEGKAVLY
                     PLTVTAGSQRVTRLVGFRTLEVETLEDDEGDPLPTFVVNGERIFAKGANWIPIDSFPS
                     RMTGERYEHLLQSMVDANMNMVRLWGGGMYEHKWFYDLCDEKGILIWHDMMFSCAIYP
                     ATDPFLSNVEEEIRYQVNRLQAHPSIALWCGNNEDVVGKTVKAADPSRRWWPSSPSRS
                     EGEHIDNRGDM"
     gene            complement(1475..>1715)
                     /locus_tag="PKN79_05455"
     CDS             complement(1475..>1715)
                     /locus_tag="PKN79_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ95242.1"
                     /translation="GIVNELVEQGISIPEQVKVIGYDNIRATSFCRIPLTTVSQSTIH
                     IGEQAAKALLQLINKERTEPLHQLIGPVLVRRHSL"
BASE COUNT          373 a          448 c          486 g          408 t
ORIGIN      
        1 tgcatatctc cacggttgtc gatgtgttca ccttcacttc ttgaagggga tgacggccac
       61 caccttcggg acgggtcggc cgctttgacc gttttgccga caacgtcctc gttgtttccg
      121 caccacaggg cgatcgaggg gtgagcttga agacgattga cctgatagcg gatctcttct
      181 tcgacgttgg agaggaaggg atcggtggcg ggatagatcg cacagctgaa catcatgtcg
      241 tgccagatga ggattccctt ctcatcacag agatcgtaga accatttgtg ttcgtacatc
      301 cctccgcccc aaagccggac catgttcatg ttcgcatcga ccatcgattg gagaaggtgc
      361 tcatagcgct ccccggtcat tcgggaaggg aaggagtcga tcggaatcca gttcgctccc
      421 ttggcgaaga tccgctcccc gttgacgacg aaggtcggca acgggtcgcc ttcatcatcc
      481 tcgagcgttt caacctccaa cgtcctgaag ccgaccagtc gagtaactct ttgagatccg
      541 gcggtgaccg tcaagggata gagcaccgcc tttccctctc cgttgggatg ccagcgccgg
      601 acatccgata cggcaaaggt caatgatgcg atattcgacc gtcccgggcc gaaggcatga
      661 gtgaccgttt gggaagaacc ggcgaccgtc gcggtgaaat caagggcttt gatcgcttca
      721 agcggggcat attcaacctc gaccgttacg tcaaatgagg atgctcccgt ttcaaccgtg
      781 gtgaatttca cgctgtcgat gaacccctcg tcgacgaact cgatcttgac cggttcgtag
      841 attccgaatg tcatgatgca cggaccccaa tcccggccgc tgtggcattg ggtcttgcgg
      901 atcaggttcc gatgtttcgc atgaaccggg tactcggaat atggaatctg cctgctttcc
      961 ctgatcgctg cattctcggc gctttcaaag atgagtttga tcgtattctc tccgacctga
     1021 aggtattcac cgatttcaaa gcgccacctt cggaattgat tgtacatcgt tccgacttga
     1081 cgatcgttga taaagagcgt gatcatcgta tcggccatcg tgaacgtgat gaacggggtt
     1141 ccacaagcga aatcttcttc agccagggtg aatggtttct ccaagaccca atcatgccgg
     1201 ccgacccact ggatctccaa ttcgttcgtt ccgacgaacg gatcggggat aaggccgttc
     1261 ttcaggaggg cagagtgaac atccccggga agagtgcagt cgatgaaggg ggtctcgagg
     1321 aacgagggac aatggggggc gattgcgtga cggtcgcgcg gggaaagctt ccaaatacca
     1381 ttcatgtcga ttgtatgcat ctgagattgc ctccatggcg agtataccac aggagagggg
     1441 agtttctcac ttatccatgg agatttgatg gtttttacaa ggagtgcctg cgaacaagga
     1501 cgggcccgat gagttgatgc aacggctccg ttcgctcctt gttgataagc tgaagaagag
     1561 ccttggcggc ctgttcaccg atgtgaatgg ttgactgact gacggtcgtc aggggaattc
     1621 gacaaaacga ggtggcccgg atgttgtcgt agccgatgac cttcacttgc tcgggaattg
     1681 aaattccctg ctcaaccagt tcattgacga ttccg
//
LOCUS       DAOPUZ010000310         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_78472_length_1705_cov_2.615758, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000310 DAOPUZ010000000
VERSION     DAOPUZ010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78472_length_1705_cov_2.615758"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PKN79_05460"
     CDS             <1..405
                     /locus_tag="PKN79_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNZ95243.1"
                     /translation="LPGEAGLDSIKHGCDVGECGACTVLIDGKPFNSCIYLAIWAEGK
                     TILTLEGLSTTKGAISDIQQAFIDEGAVQCGICTPGFIMASIPIIESDVPATRDEIKR
                     QIAGNLCRCTGYEHIVDAIEKTQKKRIAERAR"
     gene            complement(427..741)
                     /locus_tag="PKN79_05465"
     CDS             complement(427..741)
                     /locus_tag="PKN79_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95244.1"
                     /translation="MVRRDVCVYHGSEGRDVEGGAMNKDERRIVGTSITGDPIYELDP
                     DRPPLTEEEQRRMDMLAGIFDCIVVGSDGKKYVSSDEDGLYHPLKRNGYPDLSIKVPL
                     SE"
     gene            835..>1705
                     /locus_tag="PKN79_05470"
     CDS             835..>1705
                     /locus_tag="PKN79_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNZ95245.1"
                     /translation="MQPYVPALGVLFLATIVLLVTRAILKHRIKVLNAREPKPRYLTV
                     LELVHKILLPVLFLVTLSFALTLTQFSTTVRKIINISFLMIGTYIAIRAINHIVGTTF
                     INLVEKDPRRTTQANNLKVLLSFIQLLVWAAGILILLSTLGVDITTAVAGLGIGGIAV
                     AVAAQGTLGDLFSYFVILFDHPFGIGDYIVFDDKSGTVEKIGVKSTQIRVKNGEILVV
                     ANKDLTASRIHNYKRMEKRLLVATHNFPFDTPYEKLEKIPSLIAEVVSSLKSFENITL
                     DRSHFKTIGKATYE"
BASE COUNT          385 a          524 c          420 g          376 t
ORIGIN      
        1 ctccccggcg aagcggggct cgattcgatc aagcatggat gcgacgtcgg cgaatgcggg
       61 gcgtgcaccg tcctcattga cggcaaaccg ttcaactcct gcatctatct ggcgatctgg
      121 gcggaaggaa agacgattct caccctggag gggctctcga ccacaaaggg agcgatcagc
      181 gacattcagc aggcgttcat cgatgaaggg gcggttcagt gcggaatctg caccccgggc
      241 ttcatcatgg cttcgattcc gatcatcgaa agcgatgtac cggccacccg cgacgaaatc
      301 aaacggcaga tcgccggaaa cctctgccgc tgcaccgggt atgaacacat cgtcgatgcg
      361 atcgaaaaaa cgcagaagaa acggatcgcg gagcgggctc gctaggtctc agcattattc
      421 ttcctctcat tccgacagcg gcaccttgat ggagagatcg ggatacccgt tcctctttaa
      481 cggatgatac aaaccgtcct catcggagga cacatacttc ttgccatccg accccaccac
      541 gatgcaatcg aagataccgg ccaacatatc catccggcgc tgctcctcct cggtcagcgg
      601 cgggcggtcg ggatccagct cgtagatcgg atcccccgtg attgaggtgc ccacgatcct
      661 tctctcgtcc ttattcatcg ctcctccttc gacatcccgt ccttcgcttc catgatagac
      721 gcaaacgtcc cgtctgacca catcatggcc ttgcacgccg accgctggaa ctaacccgcc
      781 cattccgcta cactacggag catgaatact ataacctctt actttcctgc ctcagtgcaa
      841 ccgtacgtac cggcgctcgg cgtcctgttt cttgccacga tcgtcctgtt ggtcacccgg
      901 gcgattctca agcatcgaat caaagtactc aatgctcggg aacccaaacc ccgatacctc
      961 accgtcttgg aactggttca caagattctg cttccggtgt tgttccttgt taccctctcg
     1021 ttcgcgctca cccttacgca attcagcacg acggtgcgaa agatcatcaa catcagcttc
     1081 ctgatgatcg gaacctacat cgcgatcagg gcgatcaacc acatcgtggg aacgaccttc
     1141 atcaatctgg tggaaaagga tccgagacga accactcagg cgaacaatct caaggttctt
     1201 ttgtccttta tccagctgct cgtctgggct gccggcatcc tgattcttct ttcgactctg
     1261 ggggtggaca tcaccaccgc ggtcgccggg ctgggaatcg gcggcatcgc cgtcgccgtt
     1321 gcggcgcagg ggacgctggg cgacctcttc agctacttcg tcatcctctt cgaccatccc
     1381 ttcggaatcg gcgattacat cgtctttgat gataaaagcg gaacggttga aaagatcggg
     1441 gtgaaatcca cccagatccg ggtcaagaac ggcgagatcc tggtcgtcgc gaacaaagat
     1501 cttactgcca gccgaatcca caactacaag cggatggaga agcggcttct tgtggctacc
     1561 cataacttcc cctttgacac tccgtatgaa aaactggaga agatcccttc tttgatagcc
     1621 gaggtcgtct cctcgctcaa atcctttgaa aacatcacgt tggatcgatc tcacttcaaa
     1681 acgatcggga aagcgacata tgaat
//
LOCUS       DAOPUZ010000311         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_78677_length_1702_cov_2.564056, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000311 DAOPUZ010000000
VERSION     DAOPUZ010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78677_length_1702_cov_2.564056"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..593
                     /locus_tag="PKN79_05475"
     CDS             15..593
                     /locus_tag="PKN79_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95246.1"
                     /translation="MTEARKRNPFVAYPHAELSCVYSTLKKRLAQEGIRFESSFVAGY
                     DKRFLFSGRLRLHHHIRLSFFDTLGYFIEAARLDGPRELIESFGEVAIPAYAPHTFKR
                     DMIQVGTHYQRYLFDVFSYPSYLRFDIVGALFNQANPIVELLPARTGFMDLEAFDLGF
                     GASFGVQTPAGDLLFSVGVSIDGAWSISMGIS"
     gene            566..1531
                     /locus_tag="PKN79_05480"
     CDS             566..1531
                     /locus_tag="PKN79_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607951.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNZ95247.1"
                     /translation="MEHLNGYLLMMERYNAYGRYLQERYGHPVYRIGVDAHFTCPNRI
                     GGLGGCVFCDEAASKAVYQEGVGEAARDLEGRIRSVSKQIARGKEFIERRYGSEHFSL
                     YYQSHTNTYDSIENLKKIYQSGLDEGPFVELIVSTRPDCITEEIVELLASFKDRVEAV
                     VVELGLESSDEGSLVRLGRNHDVASYRRSVGLLRSAGIGVYTHIMLGLPKEDSSTVVK
                     TAHLLNEMGTDGVKIHDLHVLRSTVLEDWHESGEVTVASSDRHLASLVTFLRHLEPRI
                     VVARLICETPRSRRIAPRFPTPKQQIITRLSALLEEGGYRQGDLL"
BASE COUNT          360 a          428 c          499 g          415 t
ORIGIN      
        1 aaggaggtgc ttgtatgaca gaagcaagga agagaaatcc ctttgtcgcc tatccgcatg
       61 ccgagctttc ctgtgtatat tccacgctga aaaagcggtt ggcccaagag gggatccggt
      121 ttgagtcatc ttttgtcgcc gggtacgaca aacggttcct tttcagcggt cgcttgcggc
      181 ttcatcacca catccggctc tccttttttg atacgctcgg ctatttcatt gaggccgccc
      241 gtcttgatgg accgcgggaa ctgatcgaat cgtttggaga agtggccatt ccggcttacg
      301 ctccgcacac cttcaagcgg gatatgatcc aggtgggaac gcactatcag cgctacctgt
      361 tcgatgtgtt cagctatccc agctaccttc gcttcgatat tgtcggagcc ttgttcaacc
      421 aggcgaatcc gatcgttgaa ctcctgcccg ctcgaaccgg tttcatggat cttgaagcgt
      481 tcgatctcgg tttcggagcg agcttcggcg tccagactcc cgccggtgat cttctcttct
      541 ccgtcggtgt ttcgatcgat ggagcgtgga gcatctcaat gggtatctcc tgatgatgga
      601 gcggtacaac gcctacggca ggtacttgca ggagcgatac ggccatccgg tgtatcggat
      661 cggggttgac gcccacttca cctgcccgaa ccggattggg ggccttgggg ggtgcgtctt
      721 ctgtgacgag gccgcttcaa aagcggtcta tcaggagggg gtgggtgaag ccgcccggga
      781 tctggaagga cgaatacgtt ccgtctccaa gcagatcgcc cggggcaagg agttcatcga
      841 gaggcgatat gggagtgaac atttcagtct atactatcaa tcccacacca atacgtacga
      901 ttcgatcgag aacctgaaga aaatctatca aagcggattg gatgaaggac cgttcgtcga
      961 gctgatcgtc tccacccgtc ccgactgcat cacggaggag attgtcgagc tccttgcctc
     1021 tttcaaagac cgggtggagg cggtcgtcgt cgagctcgga cttgagtcga gcgatgaggg
     1081 ttcgctcgtc cggcttggaa gaaaccatga tgtggcgtca tatcgaagga gcgtcgggtt
     1141 gttacgttcg gcgggaatcg gtgtttatac acatataatg cttgggctcc cgaaagagga
     1201 ttcttcgaca gtggtaaaga ccgcccatct gttgaacgag atggggacgg atggagtgaa
     1261 aattcatgat cttcacgtcc tgcgttccac cgtactggag gattggcatg aaagtggcga
     1321 ggtgaccgtc gcttcgagcg atcgacacct cgcttcactg gtcaccttcc tgagacatct
     1381 ggaaccgagg atcgtcgtgg cgcggctgat ttgcgaaaca ccgcgaagca gacgaatagc
     1441 gccgcggttt ccgactccca agcaacaaat catcacccgg ctttcagcgt tgctggaaga
     1501 ggggggatat cgccaaggag atttgctttg acagggcata agctggaaat aattattcgc
     1561 cgaatcggta ccgtgctgat gatattcgtg gtatgctgta tagggcattt ctctttttcc
     1621 caacgggcag gaagcatatc gcttgattgg gtgccttttg gggataaggg attgcacctc
     1681 ttagtgtatg cagcctacgg gt
//
LOCUS       DAOPUZ010000312         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_79413_length_1692_cov_2.287111, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000312 DAOPUZ010000000
VERSION     DAOPUZ010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79413_length_1692_cov_2.287111"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PKN79_05485"
     CDS             complement(<1..831)
                     /locus_tag="PKN79_05485"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNZ95248.1"
                     /translation="MRKALCFGSLNIDYVYDVPHILRPGETLASTNRSIYPGGKGLNQ
                     SIALAKAGVATFHAGAVGEGGELLLETLQDSGVDTRFVRTIEGLSGHTVIQRDANGQN
                     NIILYGGSNQAITEEQIDETLQYFSSGDYLLLQNEINLIPSIMTKAAEIGMVIVLNPS
                     PIDEKLLAYPLELVDIFLLNEIEAMDIVGSESPPDELLRSLSSKFPEAQIVLTLGEEG
                     ALYLDRKGGKILKHGIFDVEVVDTTAAGDTFTGYFIATLAKTNDPQEALRVASLASAL
                     V"
     gene            complement(880..1686)
                     /locus_tag="PKN79_05490"
     CDS             complement(880..1686)
                     /locus_tag="PKN79_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HNZ95249.1"
                     /translation="MYTKDKTALLDNPGIPLAVLPTPEETFRSMADDMVDTIRERNTA
                     GKRTVFIVPVGPVGQYPYFVQRVNEERVSLKNVWFFNMDEYLDEHDRPIDFDSHLSFR
                     GFMHREVYQKIRKELVMDTKQRIFPDPDHPRLLTETLERLGGADVCYGGLGINGHVAF
                     NEPDEISVEEFKELKTRVADIAMDTQIANAIGSLNGALDAMPRRSVTIGFNEIYASRK
                     IRLYCFRDWHRAVVRHAAHGPISTRFPVTILQEHPDCRITMSSNVAEPCW"
BASE COUNT          379 a          462 c          452 g          399 t
ORIGIN      
        1 caccaaagca gacgcaagcg aagccaccct gagggcttct tgaggatcgt tcgttttggc
       61 caaggtcgca atgaagtagc cggtgaacgt gtctcccgcc gcagtggtat ccacgacctc
      121 cacatcgaag attccgtgtt taagaatctt tccacctttg cggtcaagat agagagcccc
      181 ctcctcaccg agggtgagaa cgatctgcgc ttcgggaaat tttgaagaaa gggagcgaag
      241 cagctcatcg gggggtgatt cggatccgac gatatccatc gcctcgattt cgttcagaag
      301 gaagatgtcg accagctcca agggataggc aagcagtttt tcatcgatcg gggaggggtt
      361 cagcacgatg accattccga tttccgccgc tttggtcatg atcgagggga tgaggttgat
      421 ctcattctga aggaggagat aatcaccgga agaaaagtat tgaagcgttt catcgatctg
      481 ctcttcggtg atcgcctgat tcgagcctcc gtacaggatg atgttgttct gtccgtttgc
      541 atcgcgctgg atgacggtgt gcccggacaa tccttcaatt gttcggacga atcgggtatc
      601 cactccactg tcctgaagcg tctcaagcag cagctcgccc ccttcgccga cggcgccggc
      661 gtgaaaggtg gcgactcccg cttttgcgag ggcaatcgac tgattcaggc cctttccgcc
      721 tggatagatc gatcgattcg tcgaagcgag cgtctctccc gggcgaagaa tatgcggtac
      781 gtcatagaca tagtcgatat tcaatgaacc aaaacacaac gcctttctca tgattgactc
      841 ctttctaaaa cgtatcctat caaagaaagg accggaactc taccaacaag gctcggcgac
      901 attgctgctc atcgtgattc ggcaatcagg atgctcctgc aagatcgtta ccggaaaacg
      961 ggttgaaatc ggaccgtggg cggcatggcg aaccactgcg cgatgccaat cacggaagca
     1021 gtaaagccgg atttttcttg aagcgtaaat ctcattgaat ccgatcgtta ccgatcgcct
     1081 gggcatcgca tccagcgctc cgttcaacga accgatcgca ttcgctatct gcgtgtccat
     1141 agcgatgtcc gccacccgag tcttgagttc cttgaattcc tctaccgaaa tctcatcagg
     1201 ctcgttgaac gcgacgtgac cgttgatccc gagccctccg tagcagacat ccgccccgcc
     1261 aagccgttca agggtttcgg tgaggagacg agggtgatcc ggatccggga agatccgctg
     1321 cttggtatcc atcacgagct ctttccggat cttttggtat acctcgcggt gcatgaaccc
     1381 gcggaagctc agatgactgt cgaaatcgat cggccggtca tgctcatcaa ggtactcatc
     1441 catgttgaag aaccatacgt ttttcagtga aacgcgctct tcattcaccc gctgtacgaa
     1501 ataggggtat tgccccaccg gtccgacggg gacgatgaag acggtccgct tgcctgcggt
     1561 gtttcgttcc ctgatcgtat cgaccatgtc atccgccatc gagcggaacg tctcctccgg
     1621 tgtgggaagg accgcgagcg gaatcccggg attgtccaac aatgctgttt tgtcttttgt
     1681 atagatcgcc tt
//
LOCUS       DAOPUZ010000313         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_80801_length_1674_cov_2.153181, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000313 DAOPUZ010000000
VERSION     DAOPUZ010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80801_length_1674_cov_2.153181"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="PKN79_05495"
     CDS             complement(<1..791)
                     /locus_tag="PKN79_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ95250.1"
                     /translation="MNRTRRNLILLFSSLGLLLLWQGIAKSVEIPVIMPSLGPIVRAL
                     GELASGPAFSANLLATILRAIRSFLIIVITGTVLGLIGGRNQTVSLILRPFVTTLKAT
                     PVMSVILLAFIWFSSGSVPLFSAFLMGFPVMYLQIEHGVKQLDPNLGEMCDLYDVPPK
                     TKLFYYTLPSLAPWFVAGSRATLSMIWKVVIAAEVLTVPRHGVGSRMQLAQVQLQTDV
                     VLSWTLVAIALTALGDLFFEGVVSVVAKTRSTVQRRRVDALLKRF"
     gene            complement(800..>1674)
                     /locus_tag="PKN79_05500"
     CDS             complement(800..>1674)
                     /locus_tag="PKN79_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ95251.1"
                     /translation="IREADPGTIRLGVMAGPTGFSSAGLDQPDVELQVFPSPNEVIAR
                     LANGELDMAALPTNVAANLYNKGVEIRLAAVIGNGMLSLISSKGGTDIEDLYGATLHL
                     PGAGSTPDQMARMLLAWAGLVEGKDIFFDYSVAAPAQLAQLLIAGKVDYALLPQPFVS
                     MVLNRSAQATQLLDVQELYAELTGSPTYPMSVLVVSEKFASTYPASFRKVLNGYKDSV
                     NWVNGNPDLAARKIEELGIMAKALAQSAIPHCNLVYVPASEAKSAVTFYFEQLHSLDP
                     ASIGGSVPGSGLYL"
BASE COUNT          380 a          489 c          502 g          303 t
ORIGIN      
        1 tagaaacgtt tgagaagcgc atcgaccctc cttcgctgta cggttgaacg agtcttggcc
       61 actacggaca cgacgccctc gaagaacaga tctcccagag cagtgagcgc gatcgccacc
      121 agcgtccaag agagcaccac gtcggtctga agctggactt gggcaagctg catccgcgaa
      181 ccgaccccat ggcggggaac ggtgagcact tcggcagcga tgaccacttt ccagatcatc
      241 gacaaggtcg cccggcttcc ggcgacgaac cacggggcga gggatggcag agtgtagtag
      301 aagagcttcg tcttcggcgg gacatcatac aggtcgcaca tctccccgag gttgggatcc
      361 aactgcttca cgccgtgctc gatctggagg tacatcacgg ggaagcccat cagaaatgcg
      421 gaaaagagcg gcacgctccc actggagaac cagatgaacg caagaagaat cacgctcatc
      481 accggcgtcg ccttgagggt ggtgacgaag ggcctcagga ttagcgaaac agtttgatta
      541 cgtcccccga tcagaccgag aaccgtgccg gtgatgacga tgatgagaaa agagcggatc
      601 gcccggagaa tcgtggccaa caggtttgcg gaaaaagcgg ggcccgaggc caattcaccc
      661 agcgcacgaa cgatcggtcc gagcgagggc atgatcacgg ggatctccac actctttgca
      721 atcccctgcc acagcaggag aagaccaagc gaagagaaga gaagaatcag atttcgcctg
      781 gtcctgttca tcaaatcacc tacagataca gtccgcttcc cggtacgctg ccgccgatcg
      841 aagcgggatc gaggctatgc agctgctcga agtagaacgt taccgcactc ttcgcttcgc
      901 ttgccgggac atacaccagg ttgcagtggg ggatcgcgct ttgagcaagc gctttggcca
      961 tgattccaag ctcttcgatt ttccgggcgg ccaagtcggg attgccgttc acccagttca
     1021 ccgaatcctt gtagccgttc agcacttttc tgaagctcgc aggataggtc gaggcgaact
     1081 tttcgctgac cacgaggacg ctcatcggat aggtcggact cccggtcaat tcggcgtaca
     1141 gctcttggac atccaacagt tgagtggctt gggcgcttcg attgagcacc atcgagacaa
     1201 agggttgcgg caggagagcg tagtcgacct tgccggcgat cagaagctgg gccagctgag
     1261 cgggggccgc caccgagtaa tcgaaaaaga tatcctttcc ttcaacaaga cccgcccatg
     1321 caagcagcat ccgcgccatc tggtcggggg tggagcccgc tccgggcaga tggagcgtcg
     1381 ctccatacag gtcctcgata tccgtaccgc ccttcgaaga gatcagggaa agcatcccgt
     1441 tgccgatgac cgcggcgaga cggatttcga cccctttgtt gtagaggttc gcagcgacgt
     1501 tcgtcggaag agccgccatg tccagttcgc cgttggccaa gcgcgcgatc acctcgttcg
     1561 gcgagggaaa tacctgaagc tcgacatccg gctgatccag tccggccgac gagaatccgg
     1621 tggggccggc catcacgcca aggcggatcg ttcccgggtc ggcttcccgg atcg
//
LOCUS       DAOPUZ010000314         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_81329_length_1667_cov_1.933002, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000314 DAOPUZ010000000
VERSION     DAOPUZ010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81329_length_1667_cov_1.933002"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..774
                     /locus_tag="PKN79_05505"
     CDS             46..774
                     /locus_tag="PKN79_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HNZ95252.1"
                     /translation="MKIQLETIPVWDGLNSGSECYICDLMKEAEEDALTFYLGPSVMN
                     PETRVRVNEHGFCHAHWNALVARKKAQAVALMSDTYLKRTRELLEGDINNLRKAKTAN
                     QVKKAVKKFAESLNKREAGCLVCTSMENRLERYLYTTCYLWGDDPEFATALSKGKGFC
                     LHHAGLLLQTAPKALSSDKVAEFTRSLIETQLKNLDRIAEDIHWMTQKYKSENKDKPW
                     GTGEDAHKRGVEKMVGTVRVIDPV"
     gene            complement(758..1267)
                     /locus_tag="PKN79_05510"
     CDS             complement(758..1267)
                     /locus_tag="PKN79_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95253.1"
                     /translation="MSAKNRYSDIMLFDAVRKSLGSFLSSDEILLDWFKPKASVAHAL
                     ANHLYSSLGIEESDSLWVDVGVHGADIMVHDRSGKQILGIIFSFTYLSSNQQEQLLLL
                     EQGGCRMTLGLAFLPQKEYILTYRSKKGRLDYYHYVKPSGEMNKLKEKEVRTGIDAGQ
                     LTLGIKRGR"
     gene            complement(1264..1650)
                     /locus_tag="PKN79_05515"
     CDS             complement(1264..1650)
                     /locus_tag="PKN79_05515"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNZ95254.1"
                     /translation="MGEKKHVTAEFVPGKWEFTTDGGKTFPIGKTSTPYDYLLGALSG
                     CLFATFNDLAIKMKVEWEHVSFDVNGEKKATPPTTFEWVSIDVVAKGVSDQERFTKAF
                     ETATRYCSIYTTISKVAEMKWNIEFA"
BASE COUNT          400 a          425 c          460 g          382 t
ORIGIN      
        1 gactgaggaa cgaatgaagc agttgatggt gatgaagggc aaggcatgaa gatccagctt
       61 gagacgattc cggtatggga cgggctgaac agtggaagcg aatgctatat ctgcgacctc
      121 atgaaagaag cggaagaaga tgcgcttacg ttctatctgg gcccttcggt gatgaatcct
      181 gagactcggg tccgggtcaa tgagcatgga ttctgtcatg cccactggaa cgccctcgtt
      241 gccaggaaga aagctcaggc ggtagccctg atgagcgata cctacctcaa acggaccaga
      301 gagctccttg aaggggatat caacaacctc aggaaagcaa agacggccaa tcaagtcaaa
      361 aaggcggtga agaagtttgc cgaatccctc aacaaacgag aggcggggtg cctcgtctgc
      421 acgtcgatgg aaaaccggct tgagcgctac ctctatacga cctgttatct gtggggggac
      481 gatccggagt ttgcgacagc gttgtcgaag gggaaagggt tctgtcttca tcatgcgggc
      541 cttttgcttc agacggcgcc caaagcgctt tcgtccgaca aggtcgccga gttcacccgt
      601 tctctgatcg agacacagct taagaacctc gatcggatcg ccgaggatat tcactggatg
      661 acgcagaagt acaagtcgga aaataaggac aagccttggg gcaccggcga ggatgcccat
      721 aaacggggcg tggagaagat ggtgggaacg gtgcgggtca tcgaccccgt ttgatgccga
      781 gcgtcaattg acctgcatca atgccggttc ggacttcctt ttccttgagc ttgttcatct
      841 ctccgctcgg cttcacatag tggtagtagt ccaatcggcc tttcttcgac cgataggtga
      901 ggatgtactc cttttgcggg aggaacgcca agccgagggt catcctgcac cctccctgtt
      961 ccagaagaag caattgttcc tgctggttgg aggagaggta ggtgaaggag aagatgattc
     1021 ccagaatctg cttccccgag cggtcatgca ccatgatgtc ggcgccgtgc acccctacat
     1081 ccacccaaag cgaatcgctc tcttcgatgc cgagcgagct gtacaagtga ttggccaacg
     1141 catgggcgac gctggctttc ggtttgaacc agtcaagaag gatctcatcg cttgaaagaa
     1201 aagaccctag gcttttcctg acggcgtcaa agagcatgat atcggagtat cgattcttcg
     1261 cgctcatgca aattcaatgt tccacttcat ttcagcgact ttgctgatgg tggtgtagat
     1321 cgaacagtag cgcgtggcgg tttcgaacgc cttggtgaag cgctcctgat cggaaactcc
     1381 tttggccacg acgtcgatcg aaacccattc gaaggtcgtc ggcggtgtcg ccttcttttc
     1441 accgttcaca tcgaagctca cgtgttccca ttcgactttc atcttgatcg ccagatcatt
     1501 gaatgtggca aagagacacc ccgaaagcgc ccccaaaaga taatcatagg gagtggaggt
     1561 ctttccgatc gggaaggttt ttccgccatc agtagtgaac tcccactttc caggaacaaa
     1621 ttctgcagtc acatgtttct tttcacccat ctatagctcc tgtattc
//
LOCUS       DAOPUZ010000315         1660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_81813_length_1660_cov_3.017445, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000315 DAOPUZ010000000
VERSION     DAOPUZ010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81813_length_1660_cov_3.017445"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /locus_tag="PKN79_05520"
     CDS             complement(<1..1654)
                     /locus_tag="PKN79_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNZ95255.1"
                     /translation="MGNLDFADLIGRSIELLRDAPHVRQWVHSRFEMIMVDEYQDSNT
                     AQFLLLKLLVGPETFVGVVGDDDQSIYRFRGAEVKNILTFASSFPKARTIKLEQNYRS
                     TTQILDVANSIISKNADRHDKNLWSARKGGRKPKLFYVHDEEDEARRIVTLLREDHNY
                     NGSAVLYRTNAQSVPFETTFKRYGIPYKVVGALQFYEREEVKDALALLYLLTNPKDEV
                     SFRRIINKPARGIGPGSVDKIIGFAPQTDGDLLRAAALAVKNRMLSPKAAQGAELAIR
                     LIDEGRKLIVDGNLVDGVTFLIRESGLYEHYQEQDYLNTTAKAGNLEALVNALSAYES
                     SFDGLVAFLEQLTLDRTTLGTDDPRDKEGVTLITMHNTKGLEFDRVFVTGLEEGLFPG
                     RQNEGDEDIQEERRIFYVAVTRARNELYLFSSRVRKIWGRTTFQHPSRFIADIDPALI
                     RVEGSPSLGGYNSFTRRESSGWGSSGSSMIRQGFGPGAKQFEVRHEQQGDAPLFPVGQ
                     KVYSDAYGEGEVVALRGEGEREVIDVVFATGKKASYLGRFAAL"
BASE COUNT          341 a          494 c          455 g          370 t
ORIGIN      
        1 caagggcggc aaagcggccc aggtagctgg ctttctttcc ggtggcgaag acgacgtcga
       61 tcacctcccg ttccccttca ccccgaagag cgaccacttc cccctcccca tatgcatcgc
      121 tgtacacctt ctggccgacg ggaaacaggg gggcatcccc ctgttgctca tgcctcacct
      181 cgaactgttt cgcgccgggg ccgaatcctt gacggatcat gctcgaaccg ctgctccccc
      241 atccggacga ttccctcctc gtaaacgagt tgtacccccc caaagaagga gatccttcaa
      301 cccggatcag agcgggatcg atatcggcga tgaagcgact tgggtgttgg aacgtggtgc
      361 gtccccagat cttccggacc ctcgaggaga agagatagag ctcgttccga gcccgggtga
      421 ccgcaacgta gaagattcgc cgctcctcct gaatatcctc gtccccttca ttttgacggc
      481 cgggaaagag cccttcctcc aagccggtga cgaacacccg atcgaactcc aaacccttgg
      541 tgttgtgcat cgtaatgagg gtcacccctt ccttgtctcg aggatcgtcc gttccaaggg
      601 tggttcgatc gagagtcagc tgctccagga atgcgacaag tccgtcaaac gaggactcat
      661 acgccgaaag ggcgtttacc agcgcttcga ggttgccggc cttcgctgtg gtgttgaggt
      721 agtcctgttc ctgataatgc tcgtagagac ccgactcgcg gatcaggaac gtcaccccgt
      781 ccaccagatt tccatcgaca atgagctttc ggccttcatc aatgaggcga atcgcgagct
      841 cggctccctg ggcggcttta ggggaaagca tccggttttt gaccgccaag gcggcagccc
      901 gcagaagatc cccatccgtc tgtggagcga agccgatgat cttgtcgaca ctgccggggc
      961 cgatcccccg agcgggtttg ttgatgattc ttcggaagga gacttcgtcc ttcgggttcg
     1021 tcaacaggta tagaagagcc aaggcgtcct tcacttcttc ccgttcgtag aattgaagag
     1081 ctcccaccac tttgtaggga atcccatatc gtttaaacgt cgtttcgaaa ggaacgctct
     1141 gggcgttggt tcgatagagc accgcgctgc cgttgtagtt atggtcttcc cgaagaagcg
     1201 tgacgatcct gcgagcctca tcctcttcat cgtgcacata gaagagcttg ggcttccgtc
     1261 caccctttct ggcgctccaa aggttcttat cgtgccgatc ggcattcttg gagatgatgc
     1321 tgttggccac atccaaaatc tgagtggtcg aacggtagtt ctgctccagt ttgatcgttc
     1381 ttgcttttgg aaacgaggag gcgaaggtaa ggatgttctt cacttccgcc ccccggaacc
     1441 gatagatcga ctggtcgtca tcgccgacga ccccgacgaa ggtctcggga ccgacaagga
     1501 gcttcaacag aagaaactgg gcggtgttgg agtcctgata ctcgtcgacc ataatcatct
     1561 cgaagcggga gtggacccac tgtcgcacat ggggcgcatc tcggagcaac tcgattgagc
     1621 gcccgatcag atcggcgaaa tcaaggtttc ccaccgctcg
//
LOCUS       DAOPUZ010000316         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_81946_length_1658_cov_3.429819, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000316 DAOPUZ010000000
VERSION     DAOPUZ010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81946_length_1658_cov_3.429819"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKN79_05525"
     CDS             <1..260
                     /locus_tag="PKN79_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HNZ95256.1"
                     /translation="LLPQSAVTTSRGSSSVLKRNEDGTTTRVPVTVKYLGEGISQLLS
                     GDLKAGDIVVTRRTDTSNPFASMGKGSTMSMSMESPDGAPR"
     gene            257..931
                     /locus_tag="PKN79_05530"
     CDS             257..931
                     /locus_tag="PKN79_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ95257.1"
                     /translation="MSDHVIALRNVSRYYVMGEFIVKALDGVSVSIKRGEFTSIMGPS
                     GSGKSTMMNLIGCLDTPTDGQILIDGEDTSGMNEAELAFIRNQKVGFVFQQFNLLGKL
                     TALENVVTPLLYAGYRPRERRRMAINALERVGLADRMYHRPNELSGGQKQRVAIARAL
                     VNNPSILLADEPTGALDTRTGNQIMELFDEINSEGRTVILVTHDRELGLKAKRQIYLR
                     DGKIEV"
     gene            933..>1658
                     /locus_tag="PKN79_05535"
     CDS             933..>1658
                     /locus_tag="PKN79_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ95258.1"
                     /translation="MVIENIKLAFNAMRGNKMRTALSLLGIVIGVASVVAILTIGDSA
                     SQSIKDAIAIGGLDMVTIYPSPGQRNTGIFNEEFASMLRRDVEGIDVVLPQNSSMGRI
                     RVSQEATDASVVGVLSDYGDALNLEYTQGSFFDAMDNINRRQVAVLGSSVAEELFPEQ
                     EAVGRYISLFRNQAKNYLVVGVLKSKDPTFNLSYDDSVFIPYNTYSQRFIRTTNVGAF
                     VVKVTDGFDTIAVSDRITEYLDDI"
BASE COUNT          372 a          464 c          504 g          318 t
ORIGIN      
        1 tccttttgcc tcaaagcgcc gtgaccacct cgcggggcag ttcctccgtc ttgaagcgga
       61 acgaggatgg aaccacgacc cgggttccgg tgacggtgaa gtacctcggc gaggggatca
      121 gccagctgct tagcggggat ctcaaagccg gagacatcgt ggtcacccgc cgaaccgata
      181 cgagcaatcc ctttgcctcg atgggcaagg gctccaccat gagtatgtcg atggagagcc
      241 ccgatggagc accccgatga gtgatcatgt gatagccctt cggaacgtga gccgctacta
      301 cgtgatgggc gaattcatcg tcaaagcgtt ggacggcgta tcggtctcga tcaagagggg
      361 ggagttcacc tcgatcatgg gtccttcggg gagcggcaag tcgacgatga tgaacctcat
      421 cggctgtctt gacacgccca ccgacggcca gatcctgatc gacggagagg ataccagcgg
      481 gatgaacgaa gcggagcttg ccttcatccg gaaccagaag gtgggtttcg tgttccagca
      541 gttcaacctc cttgggaaac tcaccgccct ggagaacgtg gtcaccccgc tgctctatgc
      601 cggctacagg ccgcgtgaac gaagaaggat ggcgatcaac gcgcttgagc gcgtaggcct
      661 cgccgatcgg atgtatcacc ggccgaatga gctcagcggg ggacagaagc agcgcgtcgc
      721 catcgcccgg gcgctcgtga acaacccctc gattcttctg gcggacgaac cgaccggggc
      781 gctcgatacg agaacgggga atcagatcat ggagctcttt gatgagatca acagcgaagg
      841 gcggacggtg attctggtga cccacgaccg tgaactgggt ctgaaggcga agcgtcagat
      901 ctatcttcgc gacgggaaaa tcgaggtgtg agatggtcat agaaaatatc aagctcgcct
      961 tcaatgcaat gcgcgggaac aagatgagga ccgccttatc cctgctcgga atcgtcatcg
     1021 gagtagcatc ggtggtcgcc atcctcacga tcggggacag cgcctcccaa tcgatcaagg
     1081 acgcgatcgc catcggcggc ctggacatgg tgacgatcta tccctcaccc ggacagcgga
     1141 atacgggaat cttcaatgag gagttcgcct caatgcttcg acgggatgtg gaggggatcg
     1201 atgtcgtcct gccgcaaaac tcatcgatgg gtcgcatacg ggtgagtcag gaggccaccg
     1261 acgcttcggt cgtcggcgtg ctttccgatt atggggatgc gctgaatctt gaatacaccc
     1321 agggatcatt cttcgatgcg atggacaaca tcaatcgccg ccaggtggcg gtgctcggca
     1381 gcagcgtcgc cgaggaactc tttcccgagc aggaagcggt gggccgatat atcagccttt
     1441 ttagaaacca ggcgaagaat tatctggtcg tcggcgttct gaagagcaag gatccgacgt
     1501 tcaatctttc ctacgacgac agcgtcttca tcccctacaa cacctacagc cagcgcttca
     1561 tccggacgac gaacgtcggt gcattcgtcg tgaaggtcac cgatgggttc gatacgatcg
     1621 ccgtcagcga caggatcacc gaatacctcg atgacatc
//
LOCUS       DAOPUZ010000317         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_82024_length_1657_cov_2.951311, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000317 DAOPUZ010000000
VERSION     DAOPUZ010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82024_length_1657_cov_2.951311"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKN79_05540"
     CDS             complement(<1..333)
                     /locus_tag="PKN79_05540"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNZ95259.1"
                     /translation="MGHNTFGNHFSVTTFGESHGPALGVIVDGVESGFTVDLEAIQKE
                     MDRRRPGGNPSGTERNEIDKLIILSGLYEGVTTGTPIAMILESTNQRPRDYSHLKDVF
                     RPGHADWTF"
     gene            complement(336..851)
                     /locus_tag="PKN79_05545"
     CDS             complement(336..851)
                     /locus_tag="PKN79_05545"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNZ95260.1"
                     /translation="MSHIFICGIKHSGKTTIGKLLAKRLGRRWYDLDREIERKIGEPI
                     RTFYRSFGQEAFQQLEVEVLEAIIGEENLVISLGGGAADNDPLMKMVKERGTLVYLYL
                     EEAHLLERILRGGIPPFLDEEDVEGSFHRLYERRNRRYSKDCNHLIQLPFDQAKEENV
                     ELVLSSLGLKE"
     gene            complement(853..1323)
                     /locus_tag="PKN79_05550"
     CDS             complement(853..1323)
                     /locus_tag="PKN79_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95261.1"
                     /translation="MVAIYVLPLLTLLLNFLAFGSCLRFLFSRQGLYWFIPLLLTLFL
                     IVPNALTLYTVASDPNSFISTGGILTYQPLGLSLLWYLIIITFHYALKKTIRINRYEA
                     DMRKNLHEARYQAKIESRQLADREKSRKERFAGNRSVVPRTNTHPPAWVELFED"
     gene            1389..>1657
                     /locus_tag="PKN79_05555"
     CDS             1389..>1657
                     /locus_tag="PKN79_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HNZ95262.1"
                     /translation="MKLHNEIDLELLIHRNRPREIDVRGPFYRDTTAIIHSSAFRRLK
                     HKTQVFFAPSNDHICTRMEHCLHVASIASTICKGLGLDSDLAWAIG"
BASE COUNT          375 a          447 c          454 g          381 t
ORIGIN      
        1 gaacgtccag tcggcatgac cgggacggaa aacatccttg aggtgggagt agtcccttgg
       61 gcgctgattg gtcgattcga ggatcatcgc gatcggagtt cccgtggtga caccttcata
      121 caagccgctg aggatgatca gtttgtcgat ctcgtttcgt tccgtcccgc tgggatttcc
      181 ccccggacga cggcggtcca tctctttttg aatcgcttca aggtcgacgg tgaagccgct
      241 ttcgaccccg tcgacgatga cgccgagagc cggtccgtgc gactcgccga aggttgtcac
      301 ggaaaaatgg ttgccgaatg tattgtgacc cacgtctact ccttcaagcc aagggaagaa
      361 agcaccaatt caacattctc ctccttcgct tggtcaaagg gtaattgtat caaatggttg
      421 caatccttgg aatagcgtcg attgcgccgc tcgtagagcc gatgaaacga cccttcaacg
      481 tcctcctcgt ccagaaaagg gggaattcct ccccggagaa tccgttccaa gaggtgggct
      541 tcctcgagat acaaatacac cagcgttccc cgttccttga ccatcttcat caagggatcg
      601 ttgtcggcgg ctccgccgcc aaggctgatg acgaggttct cttcaccgat gatcgcctcc
      661 agcacctcga cctccaactg ctggaaggct tcttgaccga aggagcggta aaacgttcga
      721 atcggctcac cgatcttccg ttcaatctcc cggtcaaggt cataccaccg gcggcccagc
      781 cgcttggcaa gcagctttcc gatcgtggtt tttccgctgt gcttgattcc acagataaag
      841 atgtggctca tttcaatcct cgaacagctc gacccaggct ggcgggtggg tgttggtccg
      901 gggaactacg gagcggtttc cggcaaagcg ttctttcctg cttttttccc ggtccgccaa
      961 ttggcggctt tcaatcttcg cctgatagcg ggcttcatgg aggttttttc tcatatccgc
     1021 ttcatagcga ttgattcgga ttgttttctt caacgcgtaa tgaaaggtga tgataatcaa
     1081 ataccaaagc agggaaagac cgagcggctg ataggtgagg atgccgccgg tggaaatgaa
     1141 cgagttcggg tcgctggcga ccgtgtagag ggtaagcgcg ttcggaacga tcaaaaaaag
     1201 ggtgagaagg agggggatga accaatacag cccttggcgt gaaaacagaa agcgcaggca
     1261 cgatccgaag gcgagaaagt tcagtaaaag cgtcaacaac ggtaacacat agattgcaac
     1321 catggatcct ccgctggaat aggttacttt ttccatgata ccataatcat cggatgttgg
     1381 ggaatggtat gaagttgcac aatgagatcg atcttgaact tttgatacac cgaaatcggc
     1441 cgcgggagat cgatgtccgc ggtccgtttt atcgggatac gacagcgatc atccactcgt
     1501 cggcgtttcg tcgcctcaag cacaagaccc aggtgttctt cgccccctcg aacgaccaca
     1561 tctgcacccg gatggagcac tgcctccacg tcgcctcaat cgcatcgacg atctgcaagg
     1621 ggctcgggct cgacagcgac ctggcatggg cgatcgg
//
LOCUS       DAOPUZ010000318         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_82244_length_1654_cov_3.517824, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000318 DAOPUZ010000000
VERSION     DAOPUZ010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82244_length_1654_cov_3.517824"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>1654
                     /locus_tag="PKN79_05560"
     CDS             18..>1654
                     /locus_tag="PKN79_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95263.1"
                     /translation="MGVIEPLLAEYAPFLNPYYTQDTALTGNLFFTARIGKGNVLGLD
                     GRISLDGSLSTVRLGRFESSAGFLFTAAVVEDLVDVRSAALSAEGYQLSYSGEINLTD
                     SFTQGSARLLQIDDNQLLATLGIRGRMNHVLTLDFSTPLVRELAVGMDVSLKDQRLVH
                     GAGEVHLFDMDYPFTLNVDLSLLEFSLEQEGALRISGTLMPIITIAFDAFYFTLPISR
                     TSISGKALLSIDHYSSFLFESEQISIHNLTVGPTVYSIDAQLSVSSDELFIRNARFTD
                     WVNTYSGNAHYQGSPLFSLLKHRFLQPFSFNLSIFRDESPIIQTTLIGSSNRLQSMIT
                     TNGLSLGSFVSALEGTYLDLTLLGSSDLSSEINLSGSAELRSRAFSLSTKIGAEGDSF
                     RLYDSFFRWKELTFSGDLLSIANEEARLLGSFEHIRHLSYIDQLSRLDVQVGVALPGV
                     QNLFLLPDRAKPLLTQGIGTAWITIGEPLIFGEGGFTGGTHVLDGDGTALIVDGSFLN
                     GRYEYRTRTLSARLDPSFGIGATIRADLRPESFGVKV"
BASE COUNT          335 a          483 c          408 g          428 t
ORIGIN      
        1 gtttcaatca tttctctttg ggcgtcattg agccgctgct tgccgagtat gctccatttt
       61 taaaccctta ttacactcaa gataccgcgc tgacgggaaa cctctttttt accgcccgaa
      121 tcgggaaggg caacgtcttg ggacttgacg gacggatcag tctggacggc tcgctcagca
      181 cggtgcggtt gggccgcttt gaaagctcgg cggggttcct ctttaccgcg gcagtcgtgg
      241 aagatctggt cgatgtgcga agcgccgctc tctccgccga agggtatcaa ctctcctata
      301 gcggcgagat aaacctgacc gattctttca cccagggcag cgccagactt cttcagatcg
      361 atgacaacca gttgcttgcg acgctgggca ttcgcggcag aatgaatcac gtcctaacgc
      421 ttgatttctc gaccccgctg gttcgtgagc ttgcggtggg aatggatgtc agcctgaagg
      481 atcagcggct tgttcacgga gcgggagagg tgcacctttt tgatatggac tatcccttca
      541 ccctcaacgt cgatctttca cttttggaat tcagccttga gcaggagggg gcgctccgca
      601 tcagtggaac gttgatgccg atcatcacga tagcctttga cgcgttctat ttcacccttc
      661 ccatcagccg cacctcgata agcggaaaag cccttctttc catcgaccat tactcctcat
      721 tcctctttga aagcgagcag atctccattc acaaccttac ggttggacct accgtgtata
      781 gcatagatgc ccagttgagt gtttccagcg atgagctctt tattcgcaac gctcggttca
      841 ccgattgggt caacacctac agcggcaacg cccattacca aggctcaccc ctgttctctt
      901 tgttgaaaca ccgctttctc caaccctttt ccttcaatct ctcgatcttc cgcgatgagt
      961 cgcccatcat ccaaaccacg cttatcggtt cttcaaaccg actccaatcg atgatcacaa
     1021 ccaatggtct ttccctcggc tcgttcgttt ccgcccttga aggcacctat ctggatctca
     1081 cgcttctcgg ttcgagcgat ctttcttccg aaatcaacct ctccggttca gcggagctcc
     1141 gcagccgtgc attctctctc tcgacgaaaa tcggtgccga aggcgatagc ttccgcttat
     1201 atgattcatt cttccgatgg aaagagctta ccttcagcgg ggatttgctt tcgatagcga
     1261 atgaggaagc acggcttttg ggatcctttg agcatatccg ccacctttcg tacatcgacc
     1321 agctcagccg gctcgatgtg caggtgggtg ttgcgctgcc gggagttcaa aacctcttct
     1381 tgcttcccga tcgtgcaaaa ccgcttctta ctcaaggaat cggaacggca tggatcacga
     1441 ttggcgaacc gctgatcttt ggcgaaggcg gctttaccgg aggaacccac gtcttggacg
     1501 gtgacggtac cgcactcatc gtcgacggtt cattcctgaa tggcagatat gaataccgaa
     1561 cgcgtacgct ttccgcccgg ctcgatccct ccttcggtat cggggcgacg atcagagccg
     1621 acctgagacc ggaatccttt ggtgtgaagg tgga
//
LOCUS       DAOPUZ010000319         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_82292_length_1654_cov_2.025016, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000319 DAOPUZ010000000
VERSION     DAOPUZ010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82292_length_1654_cov_2.025016"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..736
                     /locus_tag="PKN79_05565"
                     /pseudo
     CDS             101..736
                     /locus_tag="PKN79_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            783..>1654
                     /locus_tag="PKN79_05570"
     CDS             783..>1654
                     /locus_tag="PKN79_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ95264.1"
                     /translation="MKRIVTLVLIVALLVPTVLFAAGAKEAAAKETYIAVISMGYMHE
                     YWQAVRLGTENAAKELGIRTTYEAPPSEDMVDLQINMVENAIQTKPDGLLLAPLDAEA
                     LVPYIDKAVKAGIPVVLFDAGANTDTFASLVATNNENAGAIAAQNMIRLLGDKGTIGL
                     IVHSATDQSAIKRRDGFLNYMKANSKINVLPPVYSGGEHEESANLMVDLLRANPDIAG
                     VYATNEGAAVGAGIGAKEAGKAGKVTIVGFDSGAQQISFLEDGTIAGFVSQDPVTIGY
                     LGVKTLYDVLQGKPV"
BASE COUNT          365 a          476 c          449 g          364 t
ORIGIN      
        1 gtggcccttt cgggggtggt cgccggattc gctctcgcca aaggagcttc aaacttcgtc
       61 ggcattctga tcggtgcctt gatcggactg ctttgcggtc atctcggggg tcatcgtagc
      121 gtatctggaa cttcccgcgt tcattgcgac cctcggaatg atgatgagcg cccggggaat
      181 gtccttggtc cttacccaag gccggcccgt atatattcca aacaacaaga ccttcaacct
      241 gattcacaac tacaacctgt tcaaatatat ccctttgccg gtcatctact tcgcggtgat
      301 cgcgcttctc acccattttt tgctgaaacg gacatcgacc ggtcgctacc tgttcgccac
      361 cggcagcaac gaggaagcgg cccgcctgtc gtgaatcaac gtccggctcg tcaaattgtt
      421 ctcgtatggt ttctgcggct tcatgtgcgg cctttcagga gtgatccttc ttgcccgcct
      481 caactccgct caaccggcga tcggctccgg gtatgagctt gatgcgatcg ccgcggtggt
      541 gatcggggga accagcctgt cgggaggcgt cggccagatc tcgggaacga tcatcggtgt
      601 tctgatcatg agcacgatga agaatggcct gaacctgatg cacgtttcac agttctggca
      661 gcaagtcatc atcggcggtg tcgtcattct tgctgtatac atcgatataa agcggcgcga
      721 agcacagcgc ctgtaacgtt tgaatgctca cgtggtgggt aataaaaaat tggaggaaac
      781 acatgaaaag aatcgtcaca ctcgtattga tcgtggccct tctcgttccg accgttctct
      841 ttgctgccgg tgctaaggag gcggcggcca aagagacata catcgccgtc atcagcatgg
      901 ggtacatgca tgagtactgg caagcggttc gtcttggaac cgaaaatgca gcaaaggagc
      961 tgggcatccg aaccacctat gaagctccgc catccgaaga tatggtcgac ttgcagatca
     1021 acatggttga aaacgcaatt cagaccaaac ccgacggctt gttgctcgca ccccttgatg
     1081 cggaggctct ggtcccctac atcgacaaag cggtcaaggc cggaatcccg gtggttctct
     1141 ttgacgccgg agcaaacacc gacaccttcg cctcgcttgt cgcgaccaac aacgaaaatg
     1201 ccggcgccat cgccgcccag aacatgatca ggctgcttgg agacaagggc accatcggat
     1261 tgatcgtcca cagcgcaacc gatcaatcgg cgatcaagcg tcgggacggc tttttgaact
     1321 atatgaaagc caactccaag atcaacgttc ttccccccgt ctacagcggt ggagagcacg
     1381 aggaatccgc caacctgatg gtcgacctcc ttcgggcgaa cccggatatc gccggtgtct
     1441 acgccacgaa tgaaggcgcc gccgtcggtg ccggaatcgg tgccaaggaa gccggcaagg
     1501 ccggaaaagt cacgatcgtc ggcttcgact ccggagcgca gcagatctcc ttccttgagg
     1561 acggaacgat cgccggcttt gtttcccagg atccggtaac catcggatat cttggtgtaa
     1621 aaacccttta cgatgtgctt caaggcaagc cggt
//
LOCUS       DAOPUZ010000320         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_82348_length_1653_cov_2.683980, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000320 DAOPUZ010000000
VERSION     DAOPUZ010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82348_length_1653_cov_2.683980"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..>1653)
                     /gene="infB"
                     /locus_tag="PKN79_05575"
     CDS             complement(54..>1653)
                     /gene="infB"
                     /locus_tag="PKN79_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNZ95265.1"
                     /translation="RLVSLYDETIIASDEDENEELVERAPIVTVMGHVDHGKTKLLDA
                     IRSTKVAEGEYGGITQHIGAYKVNLPGKGDVVFLDTPGHSAFSMMRARGAQLTDIVIL
                     VVAANDGVMPQTREAIDHAKAANVPIVVAINKIDLPDAKPERVMQQLSDLGLMPEEWG
                     GQTLFCQISALQKLGIEELLDTVLLQAEMLELKAAVNCRAEGKVLESRVDQGRGIVAS
                     VLIERGTLRQGDYYVAGIYAGRVRAMFDDTGNKIEEAGPSTPVEIIGLSDIPGAGDPF
                     QVTEDERQARQVGAKRQELERLGESRNVKKVTLESLYSKIQEGEIQDFNVIIKGDVQG
                     SVEALQQSLEKLSNNEIRLNVIRASAGAIIESDVTLASASDALVIGFNVRPTPRAQAL
                     ADQEKIEIRKYNIIYDAIDDIRSAMEGMLAPELREIEIGTVEVRETFKVPKVGLIAGC
                     MVTKGKVKRNSYVKVIRDSVQISPERLKISSLKRFKDDAREVIEGFECGIGLEHFHDL
                     RVGDILEIYEHKEIARKLELNGQV"
BASE COUNT          319 a          483 c          438 g          413 t
ORIGIN      
        1 cgtggagatc gcttccgcta tctttgcctc aaggcgttgt cgtacaaagt cactcatacc
       61 tgtccattaa gctccagttt tctggcaatt tccttgtgtt cataaatttc gaggatatcg
      121 cctactctca gatcatggaa gtgttcgaga ccgataccgc attcaaaccc ttcgatgact
      181 tcacgggcat catccttgaa gcgtttcagg gatgagatct taagccgctc gggactgatc
      241 tggacactgt ctcgaatcac ctttacatag ctgtttcgct tgacctttcc cttggtgacc
      301 atgcaacctg cgatcaggcc gaccttcggc actttgaagg tttcgcgaac ctcgaccgta
      361 ccgatctcaa tctctcgaag ctcgggagcc aacatccctt ccatcgcgct gcgaatatca
      421 tcgatcgcat cgtaaatgat gttgtatttc cggatttcga tcttctcctg gtcggcaagc
      481 gcttgggcac ggggagtcgg tcgaacgttg aagccgatga ccaacgcatc ggatgcgctg
      541 gctaaggtca catcgctttc aatgatcgct ccggcgctgg ctcgaatgac gttgagccga
      601 atctcattgt tggaaagctt ctccagcgat tgttgaagcg cttcaaccga gccttgcacg
      661 tctcccttga tgattacgtt gaagtcttga atttcaccct cttgaatctt cgaatagagt
      721 gattcaaggg tgaccttctt cacgtttctg ctttcaccca agcgctccag ctcttgccgc
      781 ttggcgccga cttggcgagc ttggcgttca tcctcggtga cttggaacgg atcgcccgct
      841 ccgggaatat cggagaggcc gatgatctcc acaggggtgg agggaccggc ttcttcgatc
      901 ttgtttccgg tatcgtcaaa catcgcccgg acgcggccgg catagatgcc cgcaacgtaa
      961 tagtctccct gacgaagggt tccccgttca atgaggacgc tcgcgacgat accccgacct
     1021 tggtcgaccc gtgattcaag caccttgcct tcggctcggc agttcaccgc cgctttcagt
     1081 tccagcatct ccgcttggag aagcacggta tcgagcagct cctcgattcc gagtttctga
     1141 agagcggaaa tctggcagaa gagggtctgt ccgccccact cctcgggcat caggccgagg
     1201 tcggagagct gctgcatcac ccgttcgggc ttggcatcgg gaagatcgat cttgttgatg
     1261 gcgacaacga tcgggacgtt ggccgccttg gcgtggtcga tcgcctcgcg ggtttggggc
     1321 atgacgccgt cattggccgc gacgaccaga atgacgatgt cggtcagttg agcgccgcgg
     1381 gcccgcatca tgctgaatgc ggagtgcccg ggggtatcca ggaacaccac atctcctttc
     1441 cccggcagat tcactttata cgcaccgata tgctgggtga tgccgccgta ctccccttcg
     1501 gccacctttg tcgaacgaat cgcatcaagc agcttcgttt ttccatggtc gacgtgtccc
     1561 atgacggtga cgatcggagc ccgctcgaca agttcctcat tctcatcctc atcgctggcg
     1621 atgatcgtct cgtcgtagag ggagaccaat cgg
//
LOCUS       DAOPUZ010000321         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_82500_length_1651_cov_2.754386, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000321 DAOPUZ010000000
VERSION     DAOPUZ010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82500_length_1651_cov_2.754386"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..>1651
                     /locus_tag="PKN79_05580"
     CDS             65..>1651
                     /locus_tag="PKN79_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ95266.1"
                     /translation="MTSLPLFFPTLEEALSDALGGPEKRDLSDHPYHKSLKGTWDFIL
                     YDNPLSVDERVLDEKNSLSWKPVIVPGSWSIQGFDKPHYTNVIIPFEDDPPHPPQVNP
                     TGVYRTAFTVDNHWKNRRTVLEVGSAESYVELYLNGAFVGLSKDTRLEACFDLTDYLK
                     EGSNTLVLIVVRYSDSSYIEDQDQWWFGGIHRDVTLTSLPKKTLSDIKVTPTLNEALT
                     EGIIEIRTPGNQVIEALDVRVYTDAGKLIEQEILNAEEGAIVGSLKVASPTLWSSETP
                     NLYYLGISSLNEHLVIPIGFRTIELHRGNLLINKKRVLIKGVNRHEHDERTAKTISVE
                     SMVHDIKLMKQHNFNAVRTAHYPNDPRWYELCNRYGLYIIDEANIESHSNYDALCREE
                     SYAAAFLDRVQRMVRRDFHHPCIIGWSLGNEAGYGENHDAAAAWIRRYDPLRVIHYEG
                     AVRPEWGQGAHTLESLKRGSFATDLVCPMYPPIALIEEWDRTTNADRDPRPLIMSEYS
                     HAMGNSNGSLSDYWDLIRSSR"
BASE COUNT          406 a          478 c          412 g          355 t
ORIGIN      
        1 ttcgctgttt ctttcccatg ccatctggca acatccgcaa gtaacgagtc tgaaccgact
       61 tcccatgacc tcgctgcccc tgttctttcc gacgttggaa gaggctctct ccgatgccct
      121 cggaggaccg gaaaagcgag atctttcaga tcatccctat cataagagtc tgaaaggaac
      181 atgggatttc atcctttacg acaatcccct cagtgttgat gaacgcgtgc tcgatgagaa
      241 gaattccctt tcgtggaaac cggtcatcgt tcccggttcc tggagcatcc aaggctttga
      301 caaaccccac tacaccaacg tcatcattcc cttcgaggat gatccgcccc atcctcccca
      361 agtgaatccc accggtgtct atcgcaccgc gttcaccgtt gacaatcatt ggaaaaaccg
      421 aagaacggtc ttggaggttg gcagtgcgga aagctacgtc gagctctacc tcaacggagc
      481 cttcgtcggc ttgagcaagg acacccgcct cgaagcctgc ttcgatctca ccgattactt
      541 gaaggaaggc agcaacactc ttgttctcat cgtcgttcgc tacagcgaca gttcctacat
      601 cgaagatcag gatcagtggt ggttcggggg aatccatcgt gatgtaaccc ttacatccct
      661 ccccaagaaa acccttagtg acatcaaagt gactccgacg ctcaatgaag ccctgaccga
      721 aggaatcatc gagatacgca cccccggaaa tcaggtgatc gaggccttgg atgtgcgagt
      781 gtacaccgat gcagggaaac tcatcgagca ggaaattttg aatgccgagg aaggagcgat
      841 cgtcggttcc ttgaaggtcg cttcaccgac gctgtggagc agcgaaacgc ctaacctcta
      901 ttacctggga atctcttctc tcaatgagca tctggtcatt cccatcggct tcagaacaat
      961 cgagcttcat cggggaaatc tgctcatcaa caaaaagcgg gttctcatca aaggagtaaa
     1021 ccgccatgaa catgatgagc gaaccgccaa gacgatcagc gtcgaatcga tggtccacga
     1081 tatcaagctg atgaaacagc acaacttcaa tgcggtcaga accgcccact atcccaacga
     1141 tccaaggtgg tacgagctgt gcaaccggta cggcctgtac atcatcgatg aggcgaatat
     1201 cgagagccat tcaaactatg acgcgctctg ccgggaggaa tcatacgctg cggcgttcct
     1261 ggatcgagta cagcggatgg ttcgccgcga ctttcatcac ccgtgcatca tcggctggtc
     1321 gctcggcaat gaagcgggat acggagaaaa ccacgatgct gcagcggcat ggattcgccg
     1381 ctatgatccc ctcagggtca ttcattatga aggggcggtc cgtcccgaat ggggacaagg
     1441 agcccacacc cttgaaagct tgaaacgtgg atcgttcgcc acggacctcg tctgcccgat
     1501 gtatcccccg atagccttga tcgaagagtg ggaccggacc accaatgcgg accgggatcc
     1561 ccgccccttg atcatgtcgg aatacagcca tgcgatgggg aactccaacg gctccttgag
     1621 tgattactgg gatctcatcc gctcttcccg t
//
LOCUS       DAOPUZ010000322         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_82953_length_1645_cov_3.336478, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000322 DAOPUZ010000000
VERSION     DAOPUZ010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82953_length_1645_cov_3.336478"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PKN79_05585"
     CDS             complement(<1..495)
                     /locus_tag="PKN79_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ95267.1"
                     /translation="MRTLIKNVRLIMPDRVMGDARLVIRDGLIDDFGTEAYPNGEFHE
                     VIDGKGAYLSPGFVDTHVHGGNGTSFHDGNEEAIRNVLSVHLKGGTTSILPTLTSLTH
                     EQYLNILSLFADLDLRGEAVPDIEGIHMEGPYCAGSVLGAQDVKTYRTVDYREVDAYL
                     EIYPK"
     gene            complement(500..1333)
                     /locus_tag="PKN79_05590"
     CDS             complement(500..1333)
                     /locus_tag="PKN79_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ95268.1"
                     /translation="MARKRRLINSVLQSLALLFISLLFVLPIVFCVFTSFKESIEIIK
                     EVTFFPKKWSLVNYDYVFVRGAKYLKYYLNTIIITMSSVIVTLLLSALSGYSFAKLPF
                     KGSNALLASILFVLAFPLAAILIPIYIMEYNLGLLNTYIGLILPNLLLILPFSIFIMR
                     GTFINLSRELEDSAEIDGCSVVQTWWKIMLPLAQNALVIVFVSGFYSVWGEYTISKSL
                     ATKDSIMPISVALTLLKSEDWNYGVLAAAITMSIIPPITIFAIFQNRLVEGMVEGAIK
                     G"
     gene            complement(1335..>1645)
                     /locus_tag="PKN79_05595"
     CDS             complement(1335..>1645)
                     /locus_tag="PKN79_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ95269.1"
                     /translation="EAAEIDGAVGFKKLIYIIIPNLSETFAVVGIWAVLQCLKLFVTP
                     NVLTKGGPGNATIVLYQKIYNEAFMNYEMGYASSIAFILTALVMVFSILNLKISRKEE
                     "
BASE COUNT          436 a          424 c          411 g          374 t
ORIGIN      
        1 cttcggatag atttccagat acgcatcaac ctcgcgataa tccacggtgc gatacgtttt
       61 gacatcttga gccccaagca ccgatcccgc acagtaaggc ccttccatgt gaataccctc
      121 tatgtcgggg acagcttcac cgcgaagatc caaatcggca aagaggctga gaatgttaag
      181 atactgttca tgggtgagac tggtcagcgt cgggaggatc gaggtcgttc ctcccttcag
      241 atggacactc agcacatttc tgatcgcttc ttcattccca tcatgaaagc tcgtcccgtt
      301 gcctccgtgg acatgggtat cgacaaagcc ggggctcaga tacgccccct tgccatcaat
      361 aacctcatgg aactccccgt tcggatacgc ttcggtgccg aagtcatcga tcaagccgtc
      421 acggatgacc agacgggcat cgcccatcac cctgtccggc atgatgagac ggacgttttt
      481 gatgagcgtt ctcatccgac taccccttta tggcgccttc gaccatcccc tcgacaaggc
      541 gattctggaa gatcgcgaag atcgtgatcg gcggaatgat cgacatcgta atcgcggccg
      601 ccagcactcc gtaattccag tcctcactct tcaacaatgt caaggcaacg gatatcggca
      661 tgatcgaatc cttcgtcgcc aacgatttgg aaatggtgta ttcaccccac acgctgtaga
      721 acccggagac gaagacgatc accaacgcat tctgggcaag gggaagcatg attttccacc
      781 atgtctgcac gacgctgcag ccgtcgattt cggccgagtc ttccaactcg cgggaaaggt
      841 tgatgaacgt tcctcgcatg atgaagatgg agaatggaag aatcaacaga agattcggta
      901 gaatcagacc gatatatgtg ttcaagagcc caagattgta ctccatgatg taaatcggaa
      961 tgagaatcgc cgcaagcgga aaggcaagca cgaacaggat cgaggctagc agcgcgttgc
     1021 ttcctttgaa aggaagcttg gcgaatgaat atcctgaaag agcggaaaga agaagggtca
     1081 caatcaccga actcatcgtg ataatgatcg tattcagata gtatttgagg tacttcgcac
     1141 ctcggacaaa cacgtaatcg tagttgacca atgaccattt cttggggaag aacgttactt
     1201 ctttgataat ctcgatcgac tccttgaaag acgtgaagac gcagaacacg atcgggagga
     1261 cgaagagcag cgagatgaac agtaatgcca acgattggag aacggagttg atcagtcgtc
     1321 gttttctcgc catattactc ctccttcctg cttattttca agttcaggat ggaaaagacc
     1381 atgaccagcg cggtaagaat gaacgcaatg gatgaagcat aacccatctc atagttcatg
     1441 aacgcttcat tgtagatctt ctggtaaagg acgatcgtgg cgttgcccgg ccctcccttg
     1501 gtgagaacgt tcggggtcac aaagagtttg agacattgca gaaccgccca tattcccacc
     1561 acggcaaatg tttccgacag gttcggaatg atgatgtata tgagtttctt gaacccgacg
     1621 gcgccatcga tttcggccgc ctcga
//
LOCUS       DAOPUZ010000323         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_82983_length_1645_cov_2.432704, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000323 DAOPUZ010000000
VERSION     DAOPUZ010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82983_length_1645_cov_2.432704"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PKN79_05600"
     CDS             <1..280
                     /locus_tag="PKN79_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95270.1"
                     /translation="RQVYDQEGDQNSLTMQITLHRTRIKQLGSEIKGKQNQIKIQELE
                     AQIEIEKQKIEHIADQIGALRAQIDQLNSAIAKRRTLIRMLEGTGNDG"
     gene            273..1457
                     /gene="mltG"
                     /locus_tag="PKN79_05605"
     CDS             273..1457
                     /gene="mltG"
                     /locus_tag="PKN79_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNZ95271.1"
                     /translation="MADSKNSNVTTRSMKVTIGEKKSQVPPKKRNDEEKEPVKPHPPI
                     KNKSEGKVALRKPIVPPPIGKRKGYRRPMKELLLISLLAIVILAGMGAVGWNISRKAP
                     FDDEVVAVTIEAGMSATEVSFILEEAGIIDDRKHFLDYVVSRQAASLLKSGTYILGKN
                     STHEEILKALSSTGRVLELLVYPGYTLKQIDEMLTVRTGSKPGAFVKAAREVGSAYGL
                     RFVEGWFLGGTYPVEIGERSARDLALSMFFATEEVVRTLSDSPLLDRYSIEELIIIAS
                     MIQAETQDQSQMGGIASVIHNRLEKYEPLGIDATTRYELDDWENPIPVEALEQKTPYN
                     TRRKTGLPPSGIAAPSKAALHAAFYPETTPYFYYLHGFDKRIHYAVDYEEHKQNIEKY
                     RK"
     gene            complement(1487..1571)
                     /locus_tag="PKN79_05610"
     tRNA            complement(1487..1571)
                     /locus_tag="PKN79_05610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1535..1537),aa:Leu,seq:caa)
BASE COUNT          440 a          419 c          451 g          335 t
ORIGIN      
        1 gcgtcaggtg tatgatcaag aaggagatca aaactctctt acgatgcaaa tcaccttgca
       61 tcgaactaga atcaagcagt tgggctctga aatcaaggga aaacaaaacc agatcaaaat
      121 tcaggagctt gaggctcaga ttgagatcga gaagcagaag attgagcata tcgccgacca
      181 gatcggagcc ctccgcgccc aaatcgacca gctcaactcc gccatcgcca aacgacggac
      241 cttgatccgc atgctcgaag gaaccggcaa cgatggctga ttcgaagaac tccaacgtca
      301 ctacgcgctc aatgaaagta acaatagggg agaagaagtc tcaggttccg cctaagaaac
      361 ggaatgatga agagaaggaa cccgtaaaac ctcatccgcc gattaagaat aagagtgaag
      421 ggaaggtcgc gttgcgaaag ccgatcgttc cgcctccgat tgggaagcgc aaaggatacc
      481 gcaggccgat gaaggagctt ctgctcatct ctttgttggc gatcgttatc ctggccggga
      541 tgggagcggt ggggtggaac atctcgcgca aggctccatt tgatgatgaa gtagtcgctg
      601 tcacgatcga ggcgggaatg agtgcgaccg aggtaagctt tatacttgag gaagccggga
      661 tcatcgatga ccggaagcac tttctcgatt atgtcgtcag ccggcaagcc gcctccctgc
      721 tcaagagcgg cacctatatc ctcggaaaga actcaaccca tgaagagatt ctcaaagcgc
      781 tttcctctac cggtcgggtt ctggagctgc tcgtctatcc cggatatacc ctgaagcaga
      841 tcgatgagat gttgaccgtt cggacgggaa gcaaaccggg ggctttcgtg aaggccgccc
      901 gggaggtcgg ttcggcgtat gggctgaggt ttgtcgaagg gtggttcctg gggggaacct
      961 atccggtcga gataggagaa cgaagcgccc gggatctcgc tctttcgatg tttttcgcca
     1021 ccgaggaggt cgttcggacg ttgagcgatt cacctctgtt ggatcgatat tccatcgaag
     1081 agctgatcat catcgcctcg atgatccaag cggagactca ggaccaaagc caaatgggag
     1141 gtatcgcctc ggtgatccac aatcgtcttg agaagtacga gccgctcgga atcgatgcga
     1201 cgacccggta tgagttggat gattgggaaa acccgattcc cgtcgaggct ctggaacaga
     1261 agaccccgta caacacccgc agaaagacgg gcctgccccc aagcggcatc gccgccccct
     1321 ccaaagcggc tctgcatgcc gcattctatc cggagacgac cccatatttc tactatctcc
     1381 atggcttcga taagcggatt cactacgccg tcgattacga agaacacaag caaaacatcg
     1441 agaagtatcg gaaatgagaa caaggttgcc tgagcaacct tgtttctacc ggcggtcgga
     1501 gtcgaaccga cacggattat tcatccatca gattttgagt ctgacgtgta taccaatttc
     1561 accacgccgg cgcggtgaga atcatgctac caaaaggatg gtaaaaaagc aacagcgtaa
     1621 ataaaattgc tcgatcgttt attgt
//
LOCUS       DAOPUZ010000324         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_83109_length_1643_cov_3.496222, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000324 DAOPUZ010000000
VERSION     DAOPUZ010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83109_length_1643_cov_3.496222"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKN79_05615"
     CDS             complement(<1..606)
                     /locus_tag="PKN79_05615"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNZ95272.1"
                     /translation="MISSVVTTSGVLYRQHADLLHEIGDLHVYVKGQTIPLHGIDYHL
                     EDGFRLSANIPYPAIVSSGSTFFFHTIEEVLAFIRRFPHPAVQVPKMIREAAGLLASG
                     KLVAFPTETVYGLGADATNVEAVKRIFTAKERPTFDPLIVHIAHLEQLNGVVLDWDDR
                     AQTLAEHFWPGPLTLVVKKDPAIDDLVTAGSRTVAVRMPANP"
     gene            complement(645..1061)
                     /locus_tag="PKN79_05620"
     CDS             complement(645..1061)
                     /locus_tag="PKN79_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNZ95273.1"
                     /translation="MKHYRKELSFHLPHRRGLVNITSEVQKAIDESGIREGLVLVNAM
                     HITSSVFINDDESGLHHDWEVWLEGLAPEKPYSRYRHNGYEDNADAHLKRTIMGRETV
                     CAITDGKLDFGTWEQIFYYEFDGKRTKRVLIKIIGE"
     gene            1135..>1643
                     /locus_tag="PKN79_05625"
     CDS             1135..>1643
                     /locus_tag="PKN79_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ95274.1"
                     /translation="MKLLITADIHMGRIPSVGGLLNLSGTSAWKKIVDSAIEHEVDAL
                     ILVGDVVDDNELWFSVYGHILDGLNRLEEAGIRVFGVAGNHDSSVFARLSVDFPFLTI
                     LGQGGNWERVEFNGIELAGWSFSKETVAQNPFDQFTKTDSSKLTLGLLHTDLGGSQSK
                     YAPTKIEDF"
BASE COUNT          366 a          426 c          459 g          392 t
ORIGIN      
        1 cgggttggcc ggcatccgaa ccgcaacggt ccggctgcct gccgttacca ggtcatcgat
       61 cgccggatct ttcttcacca cgagggtaag gggccccggc caaaagtgtt cggccagggt
      121 ttgggcgcgg tcatcccaat ccagtacgac accgttgagt tgttcaagat gggcgatgtg
      181 gacgatcagg ggatcgaagg tggggcgttc ctttgctgtg aagatccgtt ttaccgcttc
      241 cacattggtc gcatccgctc ccaagccata caccgtttca gtgggaaacg ccaccagctt
      301 gccggaggcg aggaggcccg ccgcttccct gatcatcttg gggacttgaa cggcgggatg
      361 ggggaatctt ctgatgaagg cgaggacttc ctcaatcgtg tggaaaaaga acgtggagcc
      421 ggatgaaacg attgcaggat aggggatgtt cgccgaaagg cggaacccat cttcgaggtg
      481 ataatcgatt ccatgcaacg gtatcgtctg acccttgaca tacacatgaa gatcgccgat
      541 ttcatgaaga agatctgcgt gttgccgata gaggactccg gaagtggtga caacgctgga
      601 aatcatcacc ttactctacc caaaaacgga ttcttcactc aagctcattc accgatgatt
      661 tttatcagaa cccgcttggt ccgcttgccg tcgaactcgt agtagaagat ctgctcccac
      721 gttccgaaat ccagctttcc gtcggtgatc gcacagaccg tctcgcggcc catgatcgtc
      781 cgcttcaagt gggcgtcggc gttgtcctca tacccgttgt gccgataccg gctgtagggc
      841 ttttccggcg caagtccttc cagccatacc tcccaatcgt gatggagacc ggattcgtca
      901 tcgttgatga agacgcttga agtgatgtgc atcgcgttga ccagcacaag accttcccgg
      961 atgccgcttt catcgatcgc tttttgaact tccgaggtga tgttcaccag gcctctgcgg
     1021 tggggcagat ggaagctcaa ctcttttcga tagtgtttca tagtcgtttc tccttgctct
     1081 acgatacctg aagcggtaag gtaggtctat ggtatactca tccatggagc gaagatgaaa
     1141 ctattgataa ctgccgatat ccatatggga cggattccaa gcgtgggagg actgctgaac
     1201 ctcagtggaa cctcggcatg gaaaaagatc gtcgacagcg cgatcgaaca tgaagttgac
     1261 gccctgatcc ttgtcggaga tgtcgtggac gacaatgaac tctggttttc cgtgtatggc
     1321 catattctgg atgggctgaa ccgtcttgaa gaggcgggaa tcagagtgtt cggggttgcg
     1381 gggaatcatg actcctcggt attcgcccga ttgagcgtcg atttcccctt cctcacgatt
     1441 cttggtcaag gggggaattg ggagcgcgtg gagttcaacg ggatcgagct ggcaggatgg
     1501 tcgttctcca aagaaaccgt tgcccaaaat ccttttgatc agtttacaaa aacagattcc
     1561 tcaaagctca cgctcggtct gttgcatacc gaccttggcg gaagccaaag caagtacgct
     1621 cctacgaaga tcgaggactt cca
//
LOCUS       DAOPUZ010000325         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_83902_length_1633_cov_3.638150, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000325 DAOPUZ010000000
VERSION     DAOPUZ010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83902_length_1633_cov_3.638150"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PKN79_05630"
     CDS             <1..782
                     /locus_tag="PKN79_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNZ95275.1"
                     /translation="IIIDRADRYGLAQLYQLRGRVGRSDRQGYAYLFYPPDSSLNELA
                     IKRLRIISELTELGSGFKIALKDMEMRGTGNLLGRQQSGQVASVGLDMYIRILDEAIR
                     TLQNEGEKEEDRQVLLELDYTGFIPDSYIKTPSVKFEVYRKISSIQNDEQLDALRTEL
                     ADRYGQVPEEVENLLYIARIKILCRKLSITTLSDRRGVVRIEFGKVADLNIDKVMNLI
                     ALSNKKVRLDRNVLNIMYIEVETVSLKDKALFLLEHLERLL"
     gene            793..1521
                     /gene="trmB"
                     /locus_tag="PKN79_05635"
     CDS             793..1521
                     /gene="trmB"
                     /locus_tag="PKN79_05635"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNZ95276.1"
                     /translation="MNETIFKDFPELRVVDTREIGSDRKIKSYVLRTAGLKKREARAI
                     ETYWPRYGLSFKEEMLDYSVLFGNDNPVVIEIGFGNGAATAHIAKMRSEYNYIGLEVF
                     LTGIAKLLNAVGKEGLDNIKMMRFDAVEVLKHMIADASIAGFHIFFPDPWPKKRHVKR
                     RLIQKPFAELLARKLIKGGYIYCVTDWQEYADQMLEVFDSIPDLTNPYGGFAPPIPWR
                     LKTSFEKKGIEKEHPINEVWVEKA"
BASE COUNT          415 a          423 c          449 g          346 t
ORIGIN      
        1 cgatcatcat cgaccgggcg gaccgctacg ggcttgccca gctctaccag ctccgcggcc
       61 gggtaggccg cagcgaccgt caggggtatg cctatctctt ttatccgccg gacagctcgc
      121 tcaacgagct ggcgatcaaa aggctgcgca tcatcagtga actgaccgag ctgggcagcg
      181 gattcaagat cgccctgaaa gatatggaga tgcggggaac gggcaatctg cttggacgac
      241 agcaaagcgg acaggtcgcc tcggtcggac tggacatgta catccgaatc cttgatgaag
      301 cgattcgaac ccttcagaac gagggggaaa aagaggagga tcgccaagtc cttctggagc
      361 ttgattatac cggctttatt cccgattcct acatcaaaac tccgagcgtg aagttcgagg
      421 tctatcggaa gatctcctcc atccagaacg acgagcagct ggatgcctta cgaaccgagc
      481 tggccgaccg ctatggccag gttcccgaag aggtggagaa ccttctctac atcgcccgaa
      541 tcaagatcct ctgccggaag ctttccatca ccacgctgag cgaccgcaga ggagtcgtcc
      601 ggatcgaatt cggaaaagtg gcggatttga acatcgacaa agtgatgaac ctgatcgccc
      661 tttccaacaa gaaggtgcgg cttgatcgaa acgtgctgaa catcatgtat atcgaggtgg
      721 aaacggtgag cctgaaggac aaggcgctct ttttgctgga gcaccttgag cggcttttgt
      781 agaggtgtct tgatgaatga aacaatcttc aaggatttcc ccgagctgag agtggtcgac
      841 acccgggaaa tcggaagtga tcggaagata aaaagctatg tgctcagaac cgccggtttg
      901 aaaaagcggg aggcgagggc gatcgaaacg tattggcccc gctacggcct ttccttcaaa
      961 gaggagatgc tcgattattc cgttctcttc ggaaacgaca atccggtggt gatcgaaatc
     1021 gggttcggca atggggcggc gaccgctcat atcgccaaga tgcggagcga atacaactat
     1081 atcggcttgg aagtgtttct tacgggaatc gccaagctgc tcaacgctgt aggaaaagaa
     1141 gggttggaca atattaaaat gatgcgcttt gatgccgtcg aggtactcaa gcatatgatc
     1201 gccgatgctt caatcgccgg attccacatc ttctttcccg atccatggcc caaaaaacgc
     1261 cacgtcaagc ggcggctcat ccaaaagccg ttcgccgagc tgctggcccg gaagctcatc
     1321 aaaggcgggt atatctattg cgttaccgat tggcaggagt acgccgatca gatgcttgag
     1381 gtatttgact ccattcccga tctcacaaac ccctatggtg gattcgcccc tccgattcct
     1441 tggcgcctaa agacctcttt tgagaagaaa gggatcgaga aagaacaccc gatcaacgaa
     1501 gtgtgggttg agaaagcata agaaaaggga ggggcgaacc cctcccaagt cctacaaatt
     1561 gactctcatg caatcagaaa tgtagtgagc ttctgattgt cccggattct cgatccttac
     1621 gcctcctcct ggg
//
LOCUS       DAOPUZ010000326         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_83905_length_1633_cov_3.485425, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000326 DAOPUZ010000000
VERSION     DAOPUZ010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83905_length_1633_cov_3.485425"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="PKN79_05640"
     CDS             complement(<1..743)
                     /locus_tag="PKN79_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNZ95277.1"
                     /translation="MRRSGKRSAFLILEEYEHYLALQKRLSEATIEVYLFEAGLLLEA
                     FEDPELVTVQEIEAFIIASSEQRSLSPRSIAKTQSALRSFFTFLQLQKIRADNPLSLI
                     RTPKKEESLPQVATYDEIEHLFSVINTADYLGIRDKTLFEVIYSCGLRISEAASIDIE
                     DYQDCQLRVIGKRNKMRLIPVGEVAQASLAYYLQEARPHLLSRSKPSRALFIGRRGQR
                     LTRQAISKRFDEYAALAGLTMSVHTLRHS"
     gene            complement(727..>1633)
                     /gene="ftsZ"
                     /locus_tag="PKN79_05645"
     CDS             complement(727..>1633)
                     /gene="ftsZ"
                     /locus_tag="PKN79_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNZ95278.1"
                     /translation="DMVFITAGMGGGTGTGAAPIVAELSKSMNILTVAVVTTPFAFEG
                     KKKLTLAQAGIEKLRKHVDTLIIIPNQYLLRVVENNTPIKQAFLMADEVLYMGVQGIS
                     ELITEPGEINIDFADVRTVMKGKGDALMGIGFGEGANRAVDAARQAISNPLLESASID
                     GAKSVLVNLSGGDSLTLQEYQDVVELVTENCADDALIIAGQAYNPELGEQIKVTVVAT
                     GFERKTEQVGVENTDMEMIKRRFSTPQTHQEREHAHEPEPPSIEVFRWEDLRKQLRQG
                     ASTSSDDYTIPTVLRYGRGKDEEER"
BASE COUNT          326 a          465 c          453 g          389 t
ORIGIN      
        1 aagctgtgcc ggagggtgtg gacgctcatt gtgaggcctg ccagggcggc atactcatca
       61 aagcgcttgc tgatcgcctg ccgagtgagc cgctgccccc ggcgaccgat aaagagcgcc
      121 cgactgggtt tggagcgcga gagaagatgg ggcctcgcct cctgaagata atacgccagc
      181 gacgcctgag ccacctcgcc gacggggatg agccgcatct tgttgcgctt gccgatgacc
      241 cgaagttggc agtcctgata gtcttcaata tcgatcgatg ccgcttcgct gattctgagt
      301 ccgcaggaat aaatcacttc aaagagggtc ttgtccctga tgcccaggta gtcggcggtg
      361 ttgatgaccg agaagagatg ttcgatttca tcataggtgg ccacctgagg aagcgactcc
      421 tccttcttcg gagtccggat gagcgagagc gggttgtcag ctcggatttt ttgaagctga
      481 aggaacgtga agaaggaacg aagggcgctc tgcgtcttgg cgatgcttcg aggagacagc
      541 gatcgttgct cactcgaagc gatgatgaac gcttcgatct cttgaacggt cacgagttcg
      601 ggatcttcaa acgcttcaag caagaggcct gcctcaaaga ggtagacctc gattgttgcc
      661 tcgctgagcc gcttttgaag agcaaggtag tgctcatact cctccaagat caaaaaagcg
      721 cttcgcttac cgctcctcct catctttgcc tcggccatac cgtaaaacgg ttggaatcgt
      781 atagtcgtca ctgctggtcg aagcgccctg tcttagctgt ttccgcaagt cctcccaacg
      841 aaacacttca atcgaaggcg gttcgggttc atgggcgtgc tcccgctcct gatgggtctg
      901 cggcgttgag aatcggcgtt tgatcatctc catatcggtg ttttcgactc cgacctgctc
      961 ggtttttcgc tcaaaacccg tggcgaccac ggttactttg atctgctcgc caagctcggg
     1021 gttgtacgct tgcccggcga tgatcagcgc atcatcggca cagttttcgg taaccaattc
     1081 gacgacatcc tgatattcct gcaaggtgag gctgtctccg cccgaaaggt tcaccagcac
     1141 gcttttcgct ccgtcgatcg aagcgctttc cagcaagggg ttgctgatcg cctgacgagc
     1201 ggcatcgacg gcccggttgg cgccttcacc aaaaccgatg cccatcagcg cgtctccctt
     1261 gcctttcatc accgtacgaa cgtcggcgaa gtcgatgttg atctctcccg gttcggtgat
     1321 cagttcactg atcccctgca ctcccatgta caaaacctcg tccgccatca gaaacgcctg
     1381 cttgatcggg gtgttgttct ccacgacccg aagcaggtat tggttgggaa taatgatgag
     1441 cgtatcgaca tgctttcgca gtttttcgat tccggcctga gcgagagtca gtttcttttt
     1501 tccttcaaac gcaaagggcg ttgtcacgac cgcaacggtc agaatgttca tgctcttgga
     1561 aagctcggcg acgatcgggg cggctcccgt tcccgtcccg cctcccatcc cggcggtgat
     1621 gaagaccatg tcc
//
LOCUS       DAOPUZ010000327         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_83988_length_1632_cov_3.680406, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000327 DAOPUZ010000000
VERSION     DAOPUZ010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83988_length_1632_cov_3.680406"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKN79_05650"
     CDS             <1..191
                     /locus_tag="PKN79_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNZ95279.1"
                     /translation="SKEATASTVRVLIESTDGTESWSTIGVSKDIIQASWSALSDSIE
                     YKLIKDGVVPNGECEKEI"
     gene            200..1462
                     /gene="leuC"
                     /locus_tag="PKN79_05655"
     CDS             200..1462
                     /gene="leuC"
                     /locus_tag="PKN79_05655"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNZ95280.1"
                     /translation="MTMTQKILAKAAGKASVRAGELIMADLDLVLGNDITSPVAINEF
                     PKFGKETVFDREKVALVPDHFTPNKDIKAAEQVKAMRTFAHDHGIVNYFEVGQMGIEH
                     ALLPEKGLVGAGEVVIGADSHTCTYGALGSFATGVGSTDMASGMATGKAWFKVPSALK
                     FNLTGTLNRHVSGKDVILHIIGLIGVDGALYQSMEYSGEGVKNLSIDDRFTIANMAIE
                     AGAKNGIFPVDEVTLDYLAEHCPRTPRIFEADEDAEYEAVYEIDLSAIKSTVAFPHLP
                     SNTRPIDEVGEVKIDQVVIGSCTNGRISDLRQAAAVLKGKKVAPWVRALIFPATQKIW
                     KQAMNEGLFEIFVDAGCAVSTPTCGPCLGGHMGILAEGERAVSTTNRNFVGRMGHPKS
                     EVYLASPAVAAASAVTGYITDPNTIEEA"
BASE COUNT          377 a          469 c          509 g          277 t
ORIGIN      
        1 attcgaagga agcgaccgcc tcgacggtcc gcgtcttgat cgaatcgacc gacgggacgg
       61 agagctggtc cacgatcggg gtaagcaagg acatcattca ggcgagctgg tccgccttaa
      121 gcgacagcat cgagtataaa ctcatcaagg acggggttgt tcccaacggt gagtgcgaga
      181 aggagattta ggcgtgggca tgacgatgac acagaagatc ctcgcaaaag cggcgggcaa
      241 agcgtccgtc cgcgcaggcg agctgatcat ggcggacttg gacctggtgc tgggcaatga
      301 catcacctcc ccggtcgcga tcaacgagtt tccgaagttc gggaaggaaa cggtcttcga
      361 cagggagaag gtcgcccttg ttcccgacca tttcaccccg aacaaggaca tcaaagcggc
      421 cgagcaggtc aaagcgatgc gaacgttcgc ccacgatcat ggaatcgtca actactttga
      481 agtcggacag atggggatcg aacacgccct gctgcccgag aaaggcctgg tgggcgccgg
      541 agaagtggtc atcggagcgg acagccatac ctgcacctac ggggcgctcg gttctttcgc
      601 caccggcgtc ggctccaccg acatggccag cggaatggcc accggcaaag cgtggttcaa
      661 agtcccctcc gccctgaagt tcaacctcac gggaacactg aacaggcacg tctcgggcaa
      721 agacgtcatc ctgcacatca tcggtctcat cggggtcgac ggggcgcttt atcaatcgat
      781 ggagtacagc ggagaagggg tgaaaaacct ttcaatcgac gatcgcttca cgatcgccaa
      841 catggcgatc gaagcgggag cgaagaacgg gatctttccc gtcgacgagg taacgctcga
      901 ctatcttgcc gaacactgtc cccgcacccc ccggatcttc gaggcggacg aggacgccga
      961 atacgaggcg gtctacgaga tcgatctttc ggcgattaag agcaccgtcg cctttcccca
     1021 tcttccctcc aacacccgtc cgatcgacga ggtcggcgaa gtgaagatcg accaggtggt
     1081 gatcggttcc tgcaccaacg gtcggatcag cgacctgagg caggccgcag cggtcctaaa
     1141 ggggaagaag gtggcgccgt gggtgcgggc gttgatcttc ccggcgaccc agaagatctg
     1201 gaagcaggcg atgaacgaag ggctgtttga aatcttcgtg gatgccggct gcgccgtctc
     1261 cacccccacc tgcggcccgt gcctcggggg gcacatgggg attctggcgg aaggggagag
     1321 ggcggtgagc accacgaacc ggaacttcgt cggacggatg ggacatccca agagcgaggt
     1381 gtaccttgca agtcccgccg ttgccgcggc aagtgccgtt accgggtaca tcaccgaccc
     1441 gaacacgatc gaggaggcat aagatgagag tgttaggaac agtatttcgc tatggggaca
     1501 acgtcgacac cgacgtgatc attcccgccc ggtatctcaa cacctccaac cacgccgaac
     1561 tggcgctcca ctgcatggag gatctcgacg cgaccttcaa ggacaaggtg aagaaggggg
     1621 atatcatcgt cg
//
LOCUS       DAOPUZ010000328         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_84080_length_1631_cov_2.945431, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000328 DAOPUZ010000000
VERSION     DAOPUZ010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84080_length_1631_cov_2.945431"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="PKN79_05660"
     CDS             complement(<1..1505)
                     /locus_tag="PKN79_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95281.1"
                     /translation="MGDRRLKRISMILLTLGVLFAQLWANEAVLLTKNQVSFYEIAEY
                     RFDVRGKTKPSALRELIVPVDGDPLFASEEKLIAALDSKRQLLINTRLFTSVSYEYEF
                     IAFHNGIASFAATFFIEDSKTLLILPYPKFSNDQLGLLLGVKVYEKNILGMLGTLTLT
                     ASTAQNDGGITGWEKRKDTIDLDIAGLPLFRTKLDLSFTYERVKGGGQGAFEVDTTLS
                     HLKILDTDLAINAHADFAPDSDFTTWNPQSWGIGFTYGPFMQYGGRYTLKTTVEFDGE
                     GLRDLYLTNSITQHNVHFFSLPLLFNLFMDLDIPIDEKIVRSANISATLGFEFPLPFG
                     FRFSASTKPLAEYRNTIDFGAGDLTPVSLITSASITRSKTDWVGNFRKGSSLSFSYVR
                     QDYIQANTERDNWHIEGTASWFPLIAFDVNPSIQLTGFYAGGGVKKTFLPSKTRKLGD
                     YFRGFLSSSSALASFDDTALEWALIASVNLSMDFINFGFARSYASPFLDIG"
BASE COUNT          414 a          412 c          438 g          367 t
ORIGIN      
        1 aaaccgatgt ccaggaaggg gcttgcgtac gatcttgcaa agccgaagtt gataaaatcc
       61 atggaaagat tgacgcttgc gatcaacgcc cattcgagag ccgtgtcatc gaaggaagca
      121 agggcgcttg aggacgagag gaagccgcgg aaataatctc cgagcttccg tgtttttgaa
      181 ggcaggaagg tcttcttcac tccccctccg gcgtagaagc cggtcagctg gatcgaagga
      241 ttcacatcga aagcgatgag ggggaaccag cttgccgtgc cttcaatatg ccagttgtcc
      301 cgttcggtat tcgcctggat ataatcctga cggacatacg agaaggaaag cgagcttccc
      361 ttgcgaaagt ttccaaccca atcggtcttg cttcgggtga ttgaggcgga agtgatcagg
      421 gaaaccggcg tcagatctcc tgccccgaaa tcgatcgtat ttcgatactc cgcaagcggt
      481 ttggtcgaag cggagaaccg gaacccgaac ggtaacggaa actcaaaccc caacgtcgcg
      541 ctgatgttgg ccgagcgcac gatcttttca tcgatcggaa tatccagatc catgaaaaga
      601 ttgaacaaca gcggaagact gaagaagtgc acgttgtgct gggtaatcga attggtgagg
      661 taaagatccc gcagcccctc tccgtcgaac tcgacggtgg ttttcaaggt ataacgtccg
      721 ccgtactgca taaacggacc gtaggtaaac ccaattcccc aagattgggg gttccacgtg
      781 gtgaaatccg aatccggcgc gaaatcggcg tgcgcattga tcgcaagatc ggtgtcgagg
      841 attttcagat gcgagagggt cgtatccacc tcgaacgcac cctgtccgcc tcccttgacc
      901 cgctcgtagg tgaagctcag atcaagcttc gtcctgaaga gaggcaagcc ggcgatgtcc
      961 aagtcgatcg tatccttccg cttctcccag ccggtgatcc ctccgtcatt ttgcgcggtg
     1021 ctggccgtca gcgtaagagt tcccagcatt ccgaggatat ttttttcata gacttttact
     1081 ccaagcaaga gacccagctg gtcgttggag aacttcggat acggcaggat cagcagggtc
     1141 ttggaatcct cgatgaagaa ggttgcggca aaggaggcga ttccattatg gaaagcgata
     1201 aattcatatt cgtaggaaac ggaggtaaag agtctggtgt tgatcagcag ctgccgtttg
     1261 gagtcaagag cggcaatcag cttttcttca ctggcgaaga gcggatcgcc atcgacgggc
     1321 acgatgagct cccgaagggc gctcggtttc gtctttcccc tgacatcgaa ccgatattcg
     1381 gcaatttcat agaagctcac ctgattcttg gtgagaagaa ccgcctcgtt tgcccaaagc
     1441 tgtgcaaaga gcactccgag ggtgaggaga atcatagata ttcgcttcaa tcgacggtca
     1501 cccatacctc accacctgcc gcttaccata ggtacatatc cagcctctgg gaaaactatc
     1561 tacctgtcta gttagtatat aggtaaacca agattatgca attacgttac agcgaaacag
     1621 tatacagttt t
//
LOCUS       DAOPUZ010000329         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_84318_length_1628_cov_2.985378, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000329 DAOPUZ010000000
VERSION     DAOPUZ010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84318_length_1628_cov_2.985378"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..1246
                     /locus_tag="PKN79_05665"
     CDS             302..1246
                     /locus_tag="PKN79_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95282.1"
                     /translation="MRILQKSILFAFVLTIGTFGLFAAPLSEQPVEQVQEPPAVIVES
                     SRRSSVTLTLDFTAEGETYRYSYTQQIVQAPGTPVRLPSRSQVIFNPGQIGFLASWSD
                     GTNTYEAGETITMPDTDTSLTATYEEGVLFSDPIHEIEYYSSDRVVTVPTVPDDDEDR
                     VFVGWYDRQSDTLLTEKTYERTTSGAFFRPVYKSLVFTRIALHSYSSNAVPRNTALSL
                     SFAYRNRGDFVMNDITASLECDHGLVRILDPQPQPRTLRPNESSHHRDFFGRRTSEDS
                     FTFVIDGSVPSGSELPFTLTVTDEDGVSFSKTFTLIVQ"
     gene            complement(1301..>1628)
                     /locus_tag="PKN79_05670"
     CDS             complement(1301..>1628)
                     /locus_tag="PKN79_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNZ95283.1"
                     /translation="GKVAYIDDSTNSGTITRNKLGFLASDSVMGYNEVSTYVSTLFYF
                     LSTEYTLGEAGEDGRFIPGRCARIMVDGKEVGIFGEIHPQVLENWKSDTPTVGCEIDL
                     DTLLGY"
BASE COUNT          346 a          486 c          428 g          368 t
ORIGIN      
        1 agaatctcgg aattgatttc tccgggcaga acctcactgc aggtaaagtc cgttcattgt
       61 tggcttgcgt gaagcagagc tccggaacac aacacaatcc cgtgtttgat gccccgggtg
      121 gcaatttcat ccggggcaat tgcttatggg cttgttccgg ggtgggatac tgcaaaagtc
      181 aaggctacga taccgccggg ctccgattgt ggagagtgtg tgtacctttc ccggcaattc
      241 ttgtcaacga gagaaggctt tgctatgttc ttcggtagat cagcaacaag gagtcaacca
      301 tatgagaata cttcaaaaat ccattctttt tgcgtttgtc ctcaccatag gaaccttcgg
      361 gctgttcgcc gccccccttt cggagcagcc cgtcgaacag gtccaggaac cacccgccgt
      421 catcgtcgaa tcatcccggc gttcgtcggt caccctcacc cttgacttca ccgccgaggg
      481 agagacctac cggtactcct atacccaaca gatcgttcag gcaccgggaa cgccggttcg
      541 cctgccctcc cgcagccaag tcatcttcaa cccgggccag atcggattcc tcgcatcctg
      601 gagcgacggg acgaacacat acgaagcggg agaaacgatt accatgccgg atacggatac
      661 atcccttacg gcgacctacg aagagggggt cctcttctct gacccgatcc atgaaatcga
      721 gtactacagc tccgatcggg tggttaccgt tcctaccgtt ccggacgatg acgaggatcg
      781 ggtcttcgtc gggtggtacg atcgccaaag cgataccctt cttaccgaaa agacctacga
      841 acggacgaca agcggcgcct tcttcaggcc ggtgtataag agcctcgtct tcacccggat
      901 cgctctgcac tcgtacagca gcaatgcggt tcccagaaat accgccctca gcttgtcctt
      961 cgcttaccgt aatcggggcg atttcgtaat gaacgacatc actgcctcgt tggaatgcga
     1021 tcatggtctt gttcggatcc tcgatcctca accgcaaccc agaaccctcc ggcccaacga
     1081 aagcagtcat catcgggatt tctttggtcg aagaacctcg gaggactcgt tcaccttcgt
     1141 catcgacggt tccgttccga gcggaagcga gctgccgttc actctcaccg tgaccgatga
     1201 ggatggagtc tccttctcga agacgttcac gttgattgtg caataacctg atattcacag
     1261 attgactgtc aggcccggga catcccgggc ctgatgtcgt tcagtacccc agaagcgtgt
     1321 caaggtcgat ctcgcacccg acggtgggag tatcgctctt ccagttctcc agcacttggg
     1381 gatggatttc accgaagatg ccgacctcct tcccgtcgac catgattcgg gcgcatctgc
     1441 ccgggatgaa gcgcccgtcc tcgcccgctt cccccagggt gtactccgtc gagaggaagt
     1501 agaagagggt ggacacgtag gtggatacct cgttgtagcc catcaccgaa tcgctggcga
     1561 gaaagccgag cttgtttcgg gtgatcgttc cgctgttcgt cgaatcatcg atgtaggcga
     1621 ccttgccg
//
LOCUS       DAOPUZ010000330         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_84333_length_1628_cov_2.736173, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000330 DAOPUZ010000000
VERSION     DAOPUZ010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84333_length_1628_cov_2.736173"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PKN79_05675"
     CDS             <1..254
                     /locus_tag="PKN79_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNZ95284.1"
                     /translation="LDEMFALEKELENFTFVPALSEPKEEDKWEGEVGLITDVVRRLS
                     GDAANSEAYLCGSPGMIDACIAVLTELHMPEEHIYYDKF"
     gene            266..742
                     /locus_tag="PKN79_05680"
     CDS             266..742
                     /locus_tag="PKN79_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95285.1"
                     /translation="MKQTPEYDRIQTQMQKGVITLEGFLGDDPRKLVDIIASDTMTVH
                     NLNTTCALIAGKMEYFRDLGFEGLGEFITLDEHFEVKVDSVRGLLPSPFGGPGMYRKV
                     NTTVVNKTLGRQITYTDLHIHLIGDHCFFQGEGSTYRLEPKELVEILEVESLPSEE"
     gene            complement(830..>1628)
                     /locus_tag="PKN79_05685"
     CDS             complement(830..>1628)
                     /locus_tag="PKN79_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNZ95286.1"
                     /translation="RDEAEIAGYRDVLSIIHENYEAIPLSKNYILQLHKVLYGHLNNP
                     MAGRIKSVQNYISVSYTHGETKTLFTPVAPFETEDALERICEEYNRVVGNMELEPLLA
                     VPIFIHDFLCIHPFNDGNGRMSRLLTTLLLYRNGFYVGKYISLEAKINKHKDLYYDAL
                     QESQYGWHEGEEDFLPFVKYLLGVILSAYKDFEERFEIVEKKLPAKEMVRKAILNKIG
                     YFNKQDIRELCPSLSTSSLEGSLRALVAEGFIRREGKGKGTRYLRSD"
BASE COUNT          414 a          420 c          402 g          392 t
ORIGIN      
        1 ttcttgacga aatgttcgcc ttggaaaaag agctggagaa cttcaccttc gttccagcgt
       61 tgagcgaacc caaagaggaa gacaaatggg aaggcgaagt aggattgatc accgacgtcg
      121 tccgccgctt aagcggtgat gcggccaaca gcgaggcata cctgtgcgga agcccgggga
      181 tgatcgatgc atgtatcgct gttcttacgg agcttcacat gccggaagag cacatctact
      241 atgacaagtt ctaaggggga agatcatgaa gcagacgccc gaatatgatc gaatccagac
      301 tcagatgcag aaaggggtca tcacccttga aggatttctc ggcgatgatc cccgcaagct
      361 cgtggacatc atcgcttccg atacgatgac ggttcacaac ctcaacacca cctgcgcttt
      421 gatcgccggg aagatggagt actttcggga tctcggtttt gaaggccttg gggagttcat
      481 caccttggat gagcattttg aagtcaaggt cgattccgtc cgagggcttc ttccctcgcc
      541 cttcggaggc ccggggatgt accggaaagt gaacacgacc gtggtgaaca agacgctcgg
      601 tcgccagatc acctataccg acctgcacat ccacctcatc ggcgaccact gcttcttcca
      661 aggcgaagga tcgacctacc gcctcgagcc gaaagagctg gttgaaattc ttgaagtcga
      721 atcgcttcca agcgaggagt gagcaaggca gaaagcacga tgatgccaaa cggcgggtat
      781 gattgtcccc actttccgat tccaccgggt gcttttattg gaaagaaaat caatccgatc
      841 gcagatatct cgtgcccttg cctttccctt cccttcgtat gaacccctcg gcgaccaaag
      901 ccctgagaga tccctcaagg gaactggtgc ttaaggacgg gcacagttcc cggatatcct
      961 gtttgttgaa atagccgatc ttgttcagaa tcgccttgcg caccatttcc tttgcaggaa
     1021 gtttcttctc cacgatttca aatcgttctt caaaatcctt gtaggcggaa agaatcacac
     1081 caagaagata ttttacgaaa ggcagaaagt cctcctctcc ttcatgccag ccgtactgtg
     1141 attcctgaag ggcatcataa tagagatcct tgtgcttgtt gatcttggcc tccaaagaga
     1201 tatacttccc cacataaaat ccgttccgat ataaaagaag cgtggtcaac aggcggctca
     1261 ttcttccatt tccatcgtta aaaggatgga tgcacagaaa atcatggatg aagatcggaa
     1321 cagccagaag gggctccagc tccatattgc ctaccaccct gttatattct tcacagattc
     1381 tttccaacgc atcttccgtc tcaaacgggg caaccggggt aaaaagggtc ttcgtttcac
     1441 catgtgtata cgatacgctg atgtagttct gaacactctt gatccgtccg gccataggat
     1501 tgttcagatg gccatacaga accttatgga gctgcaggat gtagttcttg gaaaggggaa
     1561 tggcttcata gttttcatgg atgatggaaa gcacatcccg atagccggct atctccgctt
     1621 cgtctctg
//
LOCUS       DAOPUZ010000331         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_84778_length_1622_cov_2.749840, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000331 DAOPUZ010000000
VERSION     DAOPUZ010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84778_length_1622_cov_2.749840"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKN79_05690"
     CDS             <1..741
                     /locus_tag="PKN79_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNZ95287.1"
                     /translation="HGGINIIFTSDEETGSGMDGFPYEKLDCEYCYTVDGGRRYEIQS
                     ECFNAATVKVGFEGVSYHLGAARGRMVNALTMASAFIGALPQAESPEATDGRYGYYCA
                     HTIEGSIQHVDLTVYLRDFELDNLDRRIEVLKGLARTIEGLYPGGKVDLQAKHIYYNM
                     AQIAKDKPIALDTLFAAGKALGFELKNEVIRGGTDGSRMANEAGIPCPNIFTGGYNVH
                     SRYEWAALPAMVDATRLILKIIELGAAL"
     gene            738..>1622
                     /locus_tag="PKN79_05695"
     CDS             738..>1622
                     /locus_tag="PKN79_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95288.1"
                     /translation="MKRIGTLVLLLLVNSLIFSAIHVQSGPTIPGALIDHTEEALNTV
                     MGPRLKENQELRGFLTEEADRYLLTVNLDGRELMIPIPLSWEAEPSYISDSLVYDGLA
                     LLDELPPLMIDVISKTSVLVENPDPSVRVGDRFWAIDARGNRIGLLGVRRVDESRLLL
                     QQEGGKTLQVGMGLVQGPEIPVYLSMSIDAQRVFGVHAGACIPLRLYPFSLAVEGGTL
                     GGREFSLGAGVKARVSFSQLFGTGWTPFRALSFNAAVSGGGAYSLGRGWGGYVQGTFL
                     LEYALGRWIIFAGGGERMR"
BASE COUNT          313 a          459 c          514 g          336 t
ORIGIN      
        1 cacggcggaa tcaacatcat cttcaccagc gacgaggaga ccggatcggg aatggacgga
       61 ttcccctacg agaagctcga ctgcgagtac tgctacacgg tcgacggagg caggcgctac
      121 gagatccaga gcgaatgctt caacgccgcg acggtgaagg ttggctttga aggggtgagc
      181 taccacctcg gagccgcccg gggccggatg gtcaacgccc ttacgatggc gagcgccttc
      241 atcggcgccc tcccgcaggc ggagagcccc gaggcgacgg acggacggta cggctactac
      301 tgtgcccata cgatcgaagg ttcgattcag cacgtcgacc tgaccgtgta tctgcgggac
      361 ttcgagcttg acaacctcga tcggcggatc gaggtgctga agggtctcgc ccgcacgatc
      421 gaaggcctct atcccggtgg aaaggtcgat cttcaggcga agcacatcta ctacaacatg
      481 gctcagatcg ccaaggataa accgattgct ctggacaccc tcttcgccgc ggggaaggcg
      541 ctgggctttg agttgaagaa cgaggtgatc cgcggcggaa cggatggatc ccgcatggcg
      601 aacgaagcgg ggattccctg cccgaacatc ttcaccggcg gatacaacgt ccactcccgc
      661 tatgaatggg ccgcccttcc ggcgatggtc gatgcgaccc gcctgatcct caagatcatc
      721 gaactcggag ccgcattgtg aagcgaatcg ggactctggt tcttctgctc ttggtaaact
      781 cgctgatctt ttcggcgatt cacgttcaaa gcgggccgac gattcccggt gcgttgatcg
      841 accacacgga agaggccctc aacacggtga tgggcccgcg cctcaaagag aatcaagagt
      901 tgaggggttt cctcaccgaa gaggcggacc ggtatctcct gacggttaac cttgatggcc
      961 gggagctgat gattccgatt cccctttcat gggaggcgga gccgagctat atctccgact
     1021 cgctggtcta cgacggcctc gccctgcttg atgagcttcc cccgttgatg attgatgtca
     1081 tctcgaagac aagcgttctt gttgaaaacc ccgatccttc ggttcgcgtc ggcgatcgct
     1141 tctgggcgat cgacgcccgg ggaaacagga tcggcctgct tggcgtccga agggtcgacg
     1201 aaagccggct tcttttgcag caggagggcg gaaagaccct tcaggttggg atggggctcg
     1261 tgcagggacc ggagataccg gtatatctct ccatgagcat cgatgctcaa cgggtctttg
     1321 gggtccatgc aggtgcatgc attccccttc gtctctatcc attctccctc gccgttgagg
     1381 gaggcacctt gggcggtagg gagttttcgc tgggagccgg ggtaaaagct cgggtgagct
     1441 tttcccagct cttcggaacc ggctggactc cttttcgggc gctgagtttc aatgccgctg
     1501 tttcgggagg aggagcctac tcccttggca gggggtgggg agggtatgtt cagggaacct
     1561 ttcttctgga gtacgccctc ggtcgttgga tcatcttcgc cggaggcgga gagcggatgc
     1621 gg
//
LOCUS       DAOPUZ010000332         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_85106_length_1618_cov_3.284069, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000332 DAOPUZ010000000
VERSION     DAOPUZ010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85106_length_1618_cov_3.284069"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKN79_05700"
     CDS             complement(<1..444)
                     /locus_tag="PKN79_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HNZ95289.1"
                     /translation="METAMVGGSFDPIHRGHLHLIHTVASRSPYRRFILVPVSQNHFK
                     PDADPAPGSDRVAMANLAVEAYPEIYPDDPKLEFVVDDCELHREGPSYTYDTIRELYL
                     KYPIKGRLGLLLGDDLLAQLKKWHRYKELKELVRFIVIRREGATVE"
     gene            complement(444..1514)
                     /gene="obgE"
                     /locus_tag="PKN79_05705"
     CDS             complement(444..1514)
                     /gene="obgE"
                     /locus_tag="PKN79_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNZ95290.1"
                     /translation="MIGFSDETYIDVASGDGGNGAVSFRREKFVPRGGPDGGDGGRGG
                     DVVFVVSHNLRTLAHLKRQRSFRAERGQNGSSARKYGRDGVDVVIPVPPGTVLKDPES
                     GEILVDLTDHERYVFLKGGRGGQGNWHFRSSTNLAPRYARPGQKGVALRIGVELLIIA
                     DIGFVGFPNAGKSSLLNTLTNARSAVADYPFTTKVPHLGMMRHHERDIVLADIPGLIE
                     GASDGSGMGIKFLKHISRTLGLAYLIDLSDDRYLTAFDTLTEELKAFDPELVGKERVI
                     IGTKIDEEGTDVRLAELKAKYPEETVIGLCVYFNEGVDAVKEAFYRIVERVDPATVED
                     KGFQAEMDMDAHYEMEKAPWEE"
BASE COUNT          324 a          483 c          453 g          358 t
ORIGIN      
        1 ctcgacagtc gcaccttcgc gacgaatgac gatgaagcga acaagttctt tgagctcctt
       61 gtatcgatgc catttcttca attgggcgag cagatcatcg ccgagcagca ggccgagccg
      121 tcccttgatc ggatacttca ggtagagttc cctgatggta tcgtaggtgt atgagggacc
      181 ttcccggtgc agttcacagt catcgacgac gaactccagc ttgggatcat ccgggtagat
      241 ctccggatac gcttcaactg caaggttggc catcgccacc cgatcgctcc ccggagcggg
      301 atcggcgtca ggtttgaaat ggttttgact caccggtacg agaataaagc gtcgatacgg
      361 tgagcgggaa gccacggtgt ggatgaggtg caagtgaccg cggtggatcg gatcgaacga
      421 tccgccgacc attgcggttt ccatcactct tcccacggcg ctttctccat ctcgtaatga
      481 gcatccatat ccatctccgc ttgaaacccc ttgtcctcga ccgtcgcagg gtcaacccgt
      541 tccacgatcc gataaaacgc ttcttttacc gcatcgactc cctcgttgaa atagacgcag
      601 agaccgatga ccgtttcctc ggggtatttc gctttcaatt cggcgaggcg cacatccgtt
      661 ccttcctcat cgatcttcgt gccgatgatg acccgttcct ttccgacgag ctcgggatcg
      721 aacgccttca gctcttcggt gagggtgtcg aaggcggtga gataccgatc atcgctgagg
      781 tcgatcaggt aggcaagccc gagggttcgg ctgatgtgct tgaggaactt gatccccatc
      841 cccgagccgt cgcttgcccc ttcaatgaga ccgggaatat ccgccagcac gatatcgcgc
      901 tcatggtggc gcatcatccc caggtgggga actttcgtcg tgaagggata atcggcgaca
      961 gcgctgcggg cgttggtgag cgtgttgagg agactggact ttcccgcgtt cggaaagccg
     1021 acaaagccga tgtcggcgat gatcaggagc tcaaccccga ttcggagagc cacccccttc
     1081 tgtcccggcc gggcatagcg gggagccaga ttggtggacg agcggaagtg ccagttaccc
     1141 tgtccgcccc gtccgccttt gagaaaaacg tagcgctcat ggtccgtcag gtccacgagg
     1201 atctcaccgc tttcgggatc cttgagaacc gttcccgggg gaacgggaat gactacatcc
     1261 actccgtccc gaccgtactt gcgggcggat gaaccgttct gtccccgttc cgcccggaag
     1321 gagcgctgcc gcttgaggtg ggcgagcgtt ctgaggttgt gcgagacaac gaataccaca
     1381 tcgccgcccc gccctccgtc gcccccgtcc ggtccgcccc ggggaacaaa cttttcacgg
     1441 cggaaggata cagcaccgtt gcctccgtct ccggaggcaa cgtcgatata cgtttcgtca
     1501 gaaaatccaa tcatccagag gtcctcgaat gaaccttact ccgttacgat gcttatatat
     1561 cttctgtttc gtctgctgtg gaagaggaca tgtcccgcct ccttcgcaaa caaggtat
//
LOCUS       DAOPUZ010000333         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_85111_length_1618_cov_3.106846, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000333 DAOPUZ010000000
VERSION     DAOPUZ010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85111_length_1618_cov_3.106846"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>770)
                     /locus_tag="PKN79_05710"
     CDS             complement(<1..>770)
                     /locus_tag="PKN79_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNZ95291.1"
                     /translation="MILEALNNWGKTLRMRGRPKIVLAKNYEEALALYEKYQKHILGV
                     ITDVSYEMAGVHNKYAGFALAEELHRRNSELPILLQSNELSMKEAAEKSGTHFIYKRS
                     SSLFTQLASYMRTNYGFGPFIFRNPATGAEIARVETMRALQHILPDIDLTSFEYHFRN
                     EHFSRWLKAQSLYTIAAKIKALKIPEGGDVARIRDEMIETIRSYRAERTKGVIAQFSP
                     SNYDETLFFSRIGTGSLGGKGRGLAFIDMVLRQAGIPA"
     assembly_gap    771..780
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<781..1359)
                     /locus_tag="PKN79_05715"
     CDS             complement(<781..1359)
                     /locus_tag="PKN79_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNZ95292.1"
                     /translation="MYTLDPTWLPFSNMMLNHIYNVLLICNDYDRFLLEEDGRIEEEL
                     YIEYTQLRLSSPPKITYASGREEALELLETRKFELVITMLDLGPESVEDLASSIKEVE
                     PEMPIIALSPSSGHRRNKLIKGSDSIAIDYFFYWQGDPTIFLAMIKLVEDRMNLDHDI
                     AEADVGLIILVEDSVRFYSSYLPMIYTCLIQQT"
BASE COUNT          353 a          398 c          469 g          388 t
ORIGIN      
        1 ttcgccggaa tgcccgcttg gcgcaggacc atgtcgatga acgccaggcc gcggcctttg
       61 ccgcccagcg agccggtccc gatccggctg aagaagaggg tctcatcgta gtttgacggg
      121 gagaattggg cgatgacccc cttggtccgt tcggcgcggt agcttcggat cgtttcgatc
      181 atctcgtcgc ggattcgcgc cacgtcgcct ccttcgggaa tcttcaacgc cttgattttc
      241 gcggcgatcg tatagaggct ctgcgctttc agccagcggg aaaagtgttc attgcggaag
      301 tggtattcaa aggaggtcag atcaatatcc gggaggatgt gctgcagggc gcgcatcgtc
      361 tccacccggg cgatctccgc cccggtcgcg ggattccgga agatgaaggg accgaagccg
      421 tagttggtcc gcatatagga agccagctgg gtaaagaggg agctgcttcg tttgtagatg
      481 aagtgggttc cgctcttttc cgccgcttct ttcatcgaaa gctcgttgga ttgaagaaga
      541 attggaagtt cgctgttgcg gcgatggagc tcttcggcaa gggcgaaacc cgcatatttg
      601 ttgtggacgc ccgccatctc gtaggagacg tcggtgatga cccccaagat gtgtttttga
      661 tatttctcgt acaacgcgag ggcttcctca tagtttttcg ccaggacgat cttgggccgc
      721 ccccgcatcc gcagcgtttt accccagttg ttcagggctt cgagaatcat nnnnnnnnnn
      781 agtctgttga atcaggcacg tgtagatcat cggcagatac gaagaataga agcggacgga
      841 gtcttcgacg aggatgatca ggccgacatc cgcctcggcg atatcgtggt cgaggttcat
      901 ccgatcttcc accagcttga tcatcgccag gaagatcgtg ggatcgccct gccagtagaa
      961 gaagtaatcg atcgcgatcg agtcgctgcc tttgatgagc ttgtttcggc ggtggccgga
     1021 ggaaggggaa agggcgatga tcggcatctc cggctccacc tccttgatgg aggaagcaag
     1081 atcctccacc gactcgggtc cgagatcgag catcgtgatg accagctcga acttccgggt
     1141 ctcaagcagc tcgagcgcct cctcccgtcc ggaagcgtag gtgatcttcg gcggactgga
     1201 gaggcgcagc tgggtatact cgatatacag ctcctcttcg atccggccgt cctcctccag
     1261 gaggaagcgg tcgtaatcgt tgcagatgag gagtacgttg tagatgtggt tgagcatcat
     1321 gttgctgaac ggcaaccaag tcgggtcgag ggtatacatg aaaaaagtgt agcacgagtg
     1381 cataattttg catagtttca ttaatttact gtatatttta tatatccgca aaatataggt
     1441 ggaataatct ttgaattcct aaaatcaggt aggctaaatt tcaacttagg cgaattggcc
     1501 tgctttacaa accaaaaagt tttgtctatt gtgctagcca ataggagcga gatgtatgta
     1561 taagctttca gattttatga atgagctcga acgcaggaac cccgcccaac cggagttc
//
LOCUS       DAOPUZ010000334         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_85906_length_1609_cov_2.884813, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000334 DAOPUZ010000000
VERSION     DAOPUZ010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85906_length_1609_cov_2.884813"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKN79_05720"
     CDS             complement(<1..323)
                     /locus_tag="PKN79_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ95293.1"
                     /translation="MSIKTRTNYLHIAISILLAILAILFVAPIIIVFMNSFKSKLFIS
                     SEPFAFPTSETFTGYANYVEGARKINFFKAFGYSLFITTFSVTAVVGTTSMLAWYITR
                     VKSLFT"
     gene            complement(320..1162)
                     /locus_tag="PKN79_05725"
     CDS             complement(320..1162)
                     /locus_tag="PKN79_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ95294.1"
                     /translation="MDKTLRRWAPLFVLPALICFIIAFLVPMIMGLVLSFFKFSTVTD
                     GVFVGWSNYKRIFTDMDFVTSLWMNVRFTVVTVISINILAFFLALLLTQGVKGTNVYR
                     TIFFMPNLIGGIVLGWIWLLIINGILLPKNATIITDAKYGFWGLVALANWQNIGYMMV
                     IYIAGIQTINRDLIEAAQIDGANSWIRLKSVILPSVMPAITICTFLTLTSGFKIFDQN
                     LALTAGNPARQTEMLALNIYNTFYTRTNFEGVGQAKAVLFTILVAIIALTQLAATRQK
                     EVEA"
     gene            complement(1225..>1609)
                     /locus_tag="PKN79_05730"
     CDS             complement(1225..>1609)
                     /locus_tag="PKN79_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ95295.1"
                     /translation="NVGTENYLCINSQVSAEKQALADQFLTWLFSSDTGKAIVKNDLM
                     FITPFNTFKDHELPTDPLAKEVIRWMNKDGVSSMPWAFSVIPSGQWKTDFGADLGQYI
                     NGKMSWSDVAKRAVASWKTEYDLTK"
BASE COUNT          398 a          390 c          449 g          372 t
ORIGIN      
        1 gtaaagaggg atttcacccg ggtgatgtac caagccagca tgcttgtggt gccaaccact
       61 gcggtcacgc tgaacgtggt aatgaagagc gaatacccga atgctttgaa gaagttgatc
      121 tttcttgccc cctcgacgta gttcgcatac ccggtaaacg tttcggaagt gggaaaggcg
      181 aacggttcgc tggagatgaa gagcttcgat ttgaacgaat tcatgaagac gatgataatc
      241 ggagcgacaa acaggatcgc aagtatcgcc agaagaatcg agatcgcgat atgaagatag
      301 ttggttcggg tcttgatgct catgcctcaa cctccttctg cctcgttgct gcaagctggg
      361 tcagggcgat gattgcgaca aggatcgtaa agagaaccgc tttggcttgc cccacccctt
      421 cgaagttggt ccgggtgtag aaggtgttgt agatgttgag ggcgagcatc tccgtctgcc
      481 gggcgggatt gcccgctgtc aaggcaaggt tctggtcgaa gattttaaag ccgctggtca
      541 gggtaagaaa ggtacagatc gtaatcgccg gcatcaccga gggaaggatg acgctcttga
      601 ggcggatcca gctgtttgcc ccgtcgatct gggctgcttc gatcaggtca cggttaatcg
      661 tctggattcc ggcgatgtag atcaccatca tatatccgat attctgccag ttcgccaagg
      721 caacaagccc ccagaacccg tattttgcat cggtaatgat cgtcgcattc ttcggcagca
      781 ggattccatt gatgatgagg agccaaatcc atccaaggac gattccgccg atgaggttcg
      841 gcatgaagaa gatcgttcga tagacgttcg tcccttttac cccctgagta agcagcagag
      901 cgaggaaaaa cgccagaata ttgatggaga taacggtgac aacggtgaat cggacgttca
      961 tccaaagcga tgtgacgaaa tccatatcgg tgaagattcg cttatagttg ctccatccga
     1021 caaacacgcc gtcggtgacg gttgaaaact tgaaaaatga aagcaccaat cccatgatca
     1081 tcggaaccag aaacgcgatg ataaaacaga tgagagcggg taacacgaac aggggcgccc
     1141 atcgccgcaa ggtcttatcc attgatgtat ccttttatgc acagaggggc cgtttagaaa
     1201 cggccccaat gcacctatga gaaattattt agtaagatcg tattccgttt tccaggaagc
     1261 gacggcccgc ttggcgacat cgctccagct catcttgccg ttgatgtact ggccaagatc
     1321 ggcaccgaaa tcggtcttcc actggccgct tggaatgacg ctgaacgccc aaggcatgct
     1381 ggagacgccg tctttgttca tccagcggat gacttccttg gcaagcgggt cggtcggcaa
     1441 ttcatgatcc ttgaaggtgt tgaacggcgt gatgaacatc aggtcgttct tcacgatcgc
     1501 cttaccggtg tcgcttgaga agagccaggt gaggaactgg tcggcaagag cctgcttctc
     1561 ggcgctcact tggctgttga tgcagaggta attctccgtt ccgacgttc
//
LOCUS       DAOPUZ010000335         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_86586_length_1601_cov_3.128719, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000335 DAOPUZ010000000
VERSION     DAOPUZ010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86586_length_1601_cov_3.128719"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKN79_05735"
     CDS             complement(<1..271)
                     /locus_tag="PKN79_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ95296.1"
                     /translation="MFEQLKKEYNVERTYTLQDIFARNLKERRRKLGLTQAKLAGKIG
                     VSTSFITEIETSRKAPSFSTIEKIGRALEVPCWTFFCEDGDKFTGD"
     gene            complement(368..>1601)
                     /locus_tag="PKN79_05740"
     CDS             complement(368..>1601)
                     /locus_tag="PKN79_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNZ95297.1"
                     /translation="LIDGIIDYLPSPSEIPPAQGIAAKSGDPIQVPFKPDGPPLALIF
                     KIHVDREAGAMNYVRVYSGTIKKGTALMNISRKKRERVNRIMRMYADRAEELSELKAG
                     DIGVIIGFKEGRTGDTIGSEGAQILLEQIHFPVPVISIAIEPNTTSDGDKLRGALSIL
                     SQEDPTFSWRDDEETGQLIISGMGELHLEVFVNRLTKEMKIKARVGQPQVTYRESITK
                     EASGSEEFSRIIANKEHTAGVTMTVRPYRTGNGNRFINRANTRGIPHEFVEAVERGVT
                     NAFMGGIRYGYETCNVEVEVTDIAYNELTASTFAYEAASAMCFDKVANEANPVIMEPV
                     MRMTIVVPTQYVGEAISSVTSRKGIVTSIESRAFTEQIHAQAPLSELFGYSTVLRSAT
                     QGRGTFSLEFDHYQEKGV"
BASE COUNT          327 a          436 c          424 g          414 t
ORIGIN      
        1 catcaccggt gaacttgtcc ccgtcctcgc agaagaacgt ccagcaggga acttcgagag
       61 ctcgaccgat cttttcaatc gtggaaaagg agggggcttt ccgggaggtt tcaatttcgg
      121 tgatgaatga ggtggaaacc ccgatttttc cagcaagttt ggcttgggtc aaccctaatt
      181 ttcgccgtcg ctccttcaaa ttgcgggcaa aaatatcctg taatgtgtat gttcgctcca
      241 cgttgtactc cttcttcagc tgttcaaaca aataaaaaac gaaaatgtta cattaaccga
      301 tccactatcg tatattcata cacgaataat gcgaattcgc aagggtgaaa ccggttatat
      361 agaaacatta tacacctttt tcctgataat gatcaaattc caatgagaat gtgcctcttc
      421 cttgcgttgc cgaacgcaag accgtcgaat acccgaagag ctcggacagc ggagcctggg
      481 cgtgaatctg ttcggtgaat gcccgcgatt cgatcgaggt gacgattccc ttccggctgg
      541 taacgctgct gatcgcttcg ccgacatatt gggtaggaac gacgatcgtc atccgcatga
      601 ccggttccat gatgaccgga ttcgcttcgt ttgcgacctt gtcgaaacac atcgcgctcg
      661 ccgcctcgta ggcgaaggtg ctggcggtca actcattgta ggcgatatcg gtcacttcga
      721 cctcaacgtt gcacgtttcg tacccatacc ggattcctcc catgaatgcg ttggtaactc
      781 cccgttcaac cgcctcgaca aactcatggg gaatgccgcg ggtgttcgcc cggttgatga
      841 agcggtttcc atttcccgtg cggtaggggc ggaccgtcat cgttacgccg gcggtgtgtt
      901 ctttgttggc gatgattcgg ctgaattctt cgctgcccga agcttccttg gtgatcgatt
      961 cgcggtaggt gacttgaggc tggccgaccc gggctttgat cttcatttcc ttggtcaggc
     1021 ggttcacgaa cacttcgagg tggagctcgc ccattccgct gatgattaat tggccggttt
     1081 cctcatcatc ccgccaggag aaggtaggat cctcctggct gaggatgctt aaagcgccgc
     1141 gcagcttgtc accgtcgctc gtcgtgttcg gctcgatcgc gatcgagatg acgggaacgg
     1201 ggaaatgaat ctgctccagc aggatctgag ctccttcact gccgatcgta tccccggtcc
     1261 gaccttcttt gaacccgatg atcactccga tgtcgcccgc tttcaattcg ctcaattctt
     1321 cagcgcggtc ggcgtacatg cgcatgatcc ggttgacccg ttcgcgcttt ttcctggaga
     1381 tgttcatcaa cgcggtgcct ttcttgatcg taccgctgta tacccgaacg tagttcatcg
     1441 ctcccgcttc ccgatccaca tggattttga aaatcaaggc aaggggagga ccgtccggtt
     1501 tgaaggggac ttgaatcgga tccccgctct ttgccgcgat tccctgggcc ggcggtatct
     1561 cgctcgggga aggaaggtaa tcgatgatgc cgtcgatcag g
//
LOCUS       DAOPUZ010000336         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_86598_length_1601_cov_2.639069, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000336 DAOPUZ010000000
VERSION     DAOPUZ010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86598_length_1601_cov_2.639069"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKN79_05745"
     CDS             <1..366
                     /locus_tag="PKN79_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNZ95298.1"
                     /translation="INRPIYAVGISYYGVDNKHHEHPTKIQWIDEVQNQLRGKRVLLI
                     DEVDDSRVTLAYCIGELLKYEPEEIAVLVLHNKRKEKDVGFPPEIKRYFNGVETDDVW
                     IKYPWDAADIEEHTAKEEA"
     gene            363..>1601
                     /locus_tag="PKN79_05750"
     CDS             363..>1601
                     /locus_tag="PKN79_05750"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNZ95299.1"
                     /translation="MSKVVSIVGAQWGDEGKGRMIDYLAQSSQVVIRFQGGDNAGHTV
                     INDKGKFALHIIPSGIFNPDTMNIVGAGTVVNFDTMAEELASITKAGVEVDNLFIDVR
                     AHLIMPYHRQLDGAQERSKSEKAQIGTTGRGIGPCYSDKATRSGLRAGDLHDLERART
                     RLEMLLPQKNRELAFYGLPEADIDELMSLLERWAKTYRDKIIDTLPVVRDAYETGQKI
                     LLEGQLGVMRDLDWGIYPYTTSSSPTSGGAAVGSGLGPRRIDEIIGVTKVYSTSVGGG
                     PFMTELFDEDGEKLRSIGKEFGATTGRPRRCGWFDAVATEFSCWINDFTALALTKLDV
                     LDTFETIKICTGYMIGGEYYNHLPETALQELAKPVYEEHKGWMSDTTGARSWDDLPKE
                     AQAYCKRIEELVGCPIKYISV"
BASE COUNT          367 a          467 c          500 g          267 t
ORIGIN      
        1 atcaaccgcc cgatctatgc ggtggggatc tcctactacg gagtcgacaa caagcaccac
       61 gaacacccga ccaagatcca atggatcgac gaggtgcaga accagctccg gggcaaacgg
      121 gtcctgctga tcgacgaggt cgatgacagc cgggtaacct tggcgtactg catcggtgaa
      181 cttttgaagt atgaaccgga ggagatcgcc gtgctggtcc tgcacaacaa gcgcaaggaa
      241 aaggatgtgg ggttcccccc tgagatcaag cggtacttca acggagtgga aaccgatgat
      301 gtctggatca aatatccgtg ggatgcggcg gatatcgaag agcacaccgc caaggaggaa
      361 gcatgagcaa ggttgtatca atcgttggag cccaatgggg cgatgaggga aagggtcgaa
      421 tgatcgacta cctcgcccag tcttcgcagg tggtgatccg cttccagggc ggggacaacg
      481 ccggtcacac cgtcatcaac gacaagggca agttcgccct ccacatcatt ccttccggaa
      541 tcttcaatcc cgatacgatg aacatcgtcg gggcgggaac ggtagtgaac ttcgacacaa
      601 tggctgaaga gcttgcttcg atcaccaaag cgggagtcga ggtggacaac ctcttcatcg
      661 atgtccgagc ccacctgatc atgccctacc accgccagct cgacggcgcc caggagcgct
      721 cgaagagcga gaaagcccag atcggcacca ccggccgggg gatcggcccc tgctacagcg
      781 acaaggcgac gcgaagcggc ctgagggccg gcgatcttca tgaccttgag cgcgcccgaa
      841 cccgccttga gatgctgttg ccccagaaga accgggagct tgccttctac ggccttccgg
      901 aagcggatat cgatgagctg atgtccctgc tggaacggtg ggcgaaaacc taccgggaca
      961 agatcatcga cacccttccg gtcgtccggg atgcgtatga gaccgggcag aagatcctgc
     1021 ttgaagggca gctcggggtg atgagggatc tcgactgggg aatttatccc tacacgacca
     1081 gctcctcccc caccagcggc ggagcggcgg tcggcagcgg cctgggtccg cgaaggatcg
     1141 acgagatcat cggggtcacc aaggtctact cgaccagcgt cggcggcggc ccgttcatga
     1201 ccgagctgtt cgatgaggat ggggagaagc tccgctcaat cggaaaggag ttcggagcga
     1261 cgaccggccg gccccgccgg tgcggctggt tcgatgcggt ggccacggag ttctcctgct
     1321 ggatcaacga cttcaccgcc ctcgccctga ccaagctgga cgtgctggac acctttgaga
     1381 cgatcaagat ctgcaccggg tacatgatcg gcggggaata ctacaaccac cttccggaaa
     1441 ccgcccttca ggagctggcc aaaccggtct atgaagagca caaggggtgg atgagcgaca
     1501 cgacgggcgc ccgctcctgg gatgacctgc ccaaggaggc gcaggcgtac tgcaagcgga
     1561 tcgaagaact ggtaggctgt ccgatcaagt acatctccgt c
//
LOCUS       DAOPUZ010000337         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_87549_length_1590_cov_2.715961, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000337 DAOPUZ010000000
VERSION     DAOPUZ010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87549_length_1590_cov_2.715961"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PKN79_05755"
     CDS             complement(<1..462)
                     /locus_tag="PKN79_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HNZ95300.1"
                     /translation="MNLAIYYSTSHDCAANLAGEAYLMDLPYSRILYLWVNDPCVVIG
                     RFQNPHSECNLEAMKKDGVQLVRRQSGGGAVYHDRGNLCFTLIGDKETATKEENFVLV
                     IDALAELGITAEQSGRNDILFDGRKISGNAFQTTKTKFVHHGTLLVKSDLSV"
     gene            509..1219
                     /locus_tag="PKN79_05760"
     CDS             509..1219
                     /locus_tag="PKN79_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNZ95301.1"
                     /translation="MNIVLFEALEPRNTLDLNDYRAKHIKKILKLKEGDSFKAGVINT
                     STGVSTITSVGEKSIEFTYTEEKPAEPLYPITLLVGMVRPISMRRILREAVSLGVGRL
                     ILVPTDTGEKSYVQAGLYTSGEYRSILLDGAMQSGHCQLPEVVFTASLDEEYPYEGVK
                     IALDNIEGSISLDRVEMNPEQGATLAVGPERGFSDRERILLGERGFILASFGTRILRT
                     ETICSAAVGVLLSRLGFL"
     gene            1243..>1590
                     /gene="thyX"
                     /locus_tag="PKN79_05765"
     CDS             1243..>1590
                     /gene="thyX"
                     /locus_tag="PKN79_05765"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNZ95302.1"
                     /translation="MAHNVVEAAEAILDKEFKVLDHGFIRLVDYLGSDERIVQSARVS
                     YGDGTKTYRQDKGLINYLLRNDHTSPFEQVVLTFHIKLPIFVARQWIRHRTARVNEIS
                     GRYSVMKDECYVPA"
BASE COUNT          343 a          424 c          458 g          365 t
ORIGIN      
        1 caccgagaga tcgctcttca cgagcagcgt cccgtggtgg acgaacttcg tcttcgtcgt
       61 ctggaacgcg ttgccgctga tctttctgcc atcaaaaagg atatcgtttc gtccgctttg
      121 ttcggcggtg atccccagct cggcgagggc gtcgatgacg aggacgaagt tctcctcctt
      181 cgtggcggtc tccttatcac cgatcagggt gaagcagagg ttcccccggt cgtggtagac
      241 cgcccctccg ccgctttgac ggcgaaccag ttggactccg tctttcttca tcgcttcgag
      301 gttgcactcg ctgtgggggt tctgaaagcg tccgatgacg acacaagggt cgtttaccca
      361 caaatacaga atacggctat agggcaaatc catcaaatat gcttcgccgg ccagattcgc
      421 cgcgcagtcg tgactcgttg aataataaat tgccaggttc atgttcaaga gtgtaccggg
      481 accgtgctcg tcatgctata ctctgcgcat gaatatcgtg ctctttgagg cgttggaacc
      541 cagaaacacc cttgacctga atgattaccg cgccaaacac atcaaaaaga tcctgaagct
      601 gaaggaaggc gactccttca aagccggggt gatcaatacc tccaccggcg tttccacgat
      661 cacgagcgtg ggggagaaga gcatcgagtt cacctatacc gaggaaaaac cggctgagcc
      721 gctgtacccg atcaccctgc tggtcgggat ggtcaggccg atcagcatgc ggagaatcct
      781 gcgcgaagcg gtctcattgg gggttgggcg gctgattctc gtcccgacgg acaccgggga
      841 gaagtcctac gtccaagcgg ggctgtacac gagcggcgag taccgctcga ttctccttga
      901 cggggcgatg cagagcggtc attgtcagct ccccgaagtc gtctttaccg cttctctgga
      961 tgaggaatat ccgtatgagg gagttaagat cgctttggat aacatcgaag gttcgataag
     1021 ccttgatcgc gttgaaatga atccggagca aggagccacc cttgcggtgg ggccggaacg
     1081 ggggttcagc gaccgcgagc ggattcttct cggggagcgg gggttcatcc tcgcttcgtt
     1141 cggaacgcgg attcttcgga ccgagacgat ctgcagcgcc gccgtcgggg tcttgctcag
     1201 tcgtttgggt ttcctttaaa ttggaaacgg aggaggtaag ctatggcaca taacgtggtt
     1261 gaagcggcgg aagcgattct ggacaaggag ttcaaggttc tcgatcatgg gttcatccgc
     1321 ctggtcgact atcttggaag cgatgaacgg attgttcaaa gcgcccgggt ctcctacgga
     1381 gacggaacga aaacgtatcg ccaggacaag ggattgatca actacctttt gcgcaacgat
     1441 catacctccc cctttgaaca ggttgtcctt acctttcata ttaaacttcc catctttgtg
     1501 gccaggcagt ggattcgcca ccgaaccgcc cgtgttaacg agatatcagg tcgctattcc
     1561 gttatgaagg acgaatgcta tgttccggca
//
LOCUS       DAOPUZ010000338         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_87947_length_1585_cov_3.436601, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000338 DAOPUZ010000000
VERSION     DAOPUZ010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87947_length_1585_cov_3.436601"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PKN79_05770"
     CDS             <1..280
                     /locus_tag="PKN79_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNZ95303.1"
                     /translation="GEPEFIDESFFEDDPPPITESSVGLSDAITDEELMEQALQIVVE
                     RKGASASFLQRRLKIGYNRAARLVEQMEEEGWVGPANGSKPRELLRIP"
     gene            281..1081
                     /locus_tag="PKN79_05775"
     CDS             281..1081
                     /locus_tag="PKN79_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNZ95304.1"
                     /translation="MYASRNGVIIPKEEAVVPITSREVQSGFYVYESLRVLGGFVTRI
                     NDHLDRLLASAKGIHLTHPFTKESIHRWVDELIEAEHIGDATVRITLYGGADPVLFIV
                     PVELLRYPDSYYRDGVKAITFEGERLFPTHKSGSLLLNYVALEEAKRFGGFEALLVDR
                     NGRVLEGTRSNFFAFSNNTLHTADEDLVLGGITRSEVLRCAKELGFMIDLNPIYLEDL
                     FAGRFTEVFISATSMAAMPLHTIDDLRFSAPFERTRALHDCVRARQWQ"
     gene            1189..>1585
                     /locus_tag="PKN79_05780"
     CDS             1189..>1585
                     /locus_tag="PKN79_05780"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ95305.1"
                     /translation="MNFSELPLHEQVLTGIERTGFEQCTDVQQRVLPVTLAGKDVMVQ
                     SKTGSGKTAIYAITILQSFITAQADNREVKALIIAPTRELAVQIKADIDELAGGVKNF
                     RVGCFFGGVGYAKQDEWIEKGLDVYVATPG"
BASE COUNT          350 a          411 c          463 g          361 t
ORIGIN      
        1 aggggagccg gagttcatcg atgagtcgtt ctttgaggac gatcctcccc cgatcaccga
       61 gagcagcgtc gggctcagtg atgcgatcac cgatgaggag ctgatggaac aagcacttca
      121 aatcgtggtg gaacggaaag gtgccagcgc ctccttcctg caaaggcggt tgaagatcgg
      181 atacaaccgg gcggcccgcc tcgtcgagca gatggaagaa gaagggtggg tcggaccggc
      241 gaacggttca aagccgcgtg agctgttgag gatcccctaa atgtacgcat cgagaaacgg
      301 ggtgattatc cccaaggaag aggcggtcgt gccgatcacc tcccgcgaag tccagagcgg
      361 gttttacgtg tatgagtctt tgcgggttct gggcggattc gtcacccgca tcaacgacca
      421 ccttgatcga ttgcttgcct ccgcaaaagg aattcatttg acccatccct ttacgaagga
      481 atcgatccac cgctgggtgg atgaactcat cgaagcggaa cacattggcg atgcgaccgt
      541 tcgaatcacc ctctatggcg gggcggatcc ggttctcttc atcgttccgg ttgaactttt
      601 gcgttatccc gattcctatt accgggatgg agtgaaggca atcacctttg aaggagagcg
      661 gttgtttccc acccataaga gcgggagtct gctgctcaac tatgttgccc ttgaagaagc
      721 gaagcggttc ggaggattcg aggcgttgct ggtcgatcgg aacgggcggg tgctggaagg
      781 aacgcgcagc aacttcttcg cattctccaa caatacgctt catacggcgg atgaggatct
      841 cgtccttggc ggaatcaccc gaagcgaggt gctgcgctgt gccaaagagc tcggattcat
      901 gatcgatctg aatcctatct atctggaaga tctgtttgcc ggccgcttta ccgaagtgtt
      961 catttcagcg accagcatgg cggcgatgcc gcttcacacg atcgatgacc ttcgtttttc
     1021 cgctcccttt gaacggaccc gggcgctcca cgattgcgtg cgcgcccgtc agtggcaata
     1081 gcgggaattg gtggacagag cttctctttt actttatcgt tgaaggaggc gccggcgcct
     1141 gtcttgttgt gattatgtta cttcttataa aggtgaatga tataccctat gaatttttct
     1201 gaattacccc tccatgagca ggtcctcacc ggtattgaac ggaccggctt tgaacagtgc
     1261 accgatgtcc agcagcgggt gcttccggta acacttgccg gaaaggacgt gatggttcaa
     1321 tcgaaaaccg gcagcggaaa gacagccatc tacgccatta cgattctcca aagctttatt
     1381 accgcccaag ccgacaacag agaggtcaaa gccctcatca tcgctccgac ccgggagctg
     1441 gcggtgcaaa tcaaggcgga tatcgatgaa ttggccggcg gcgtgaagaa ctttcgagtc
     1501 ggctgttttt tcggtggcgt cggctatgcc aagcaggatg aatggatcga gaagggcctt
     1561 gacgtctatg tggcgacacc cgggc
//
LOCUS       DAOPUZ010000339         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_88298_length_1581_cov_4.063565, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000339 DAOPUZ010000000
VERSION     DAOPUZ010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88298_length_1581_cov_4.063565"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1571)
                     /gene="gdhA"
                     /locus_tag="PKN79_05785"
     CDS             complement(234..1571)
                     /gene="gdhA"
                     /locus_tag="PKN79_05785"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNZ95306.1"
                     /translation="MYNLSDFMNELERRNPAQPEFIQATREVLESIIDAVNENPVYEK
                     HRILERLTEADRIFSFRVEWEDDEGNLQVNRGYRVQFNNALGPYKGGLRFHASVTLGT
                     LKFLAFEQTLKNSLTTLPMGGGKGGSDFSPKGKSDGEIMRFCRSYMSELQKYIGPDTD
                     VPAGDIGVGAREIGYLYGQYKRLRDENTGVLTGKGLNYGGSILRPEATGFGAMYFANS
                     MLALRGDSLTGKTVALSGFGNVAWGAAQKATHLGAKVVTISGPDGYIYDEEGVGTPEK
                     WAYMSYLRLSNNDVVEPYAKEFGAVFVPRKKPWEVKVDFAFPCAIQNELSLADAKALV
                     ANGVQGVVEISNMGCQADAMYYFLEKKILFAPGKAANAGGVAVSGLEMTQNAMHIAWT
                     AEEVDEKLKEIMLKIHNACVKEGTQPDGYINYVKGANITGFRKVADTMVQLGY"
BASE COUNT          318 a          462 c          416 g          385 t
ORIGIN      
        1 gtagcagttg gataccaacc ccatacaatc cggggcgtaa ttctttgcca agtaggatga
       61 ttcctcgccg ccgggtggct cccacactgt gtgcaagaag cggaaattcc ggataatttc
      121 ctgaaaacat cgatccggac gatgaacaaa tctccgcttc cgtcgagctt ctagtgtcat
      181 gaaaagaggc gacctccaaa aggtcgccgg aaaatgtaca aagttggaca aggttaatag
      241 ccgagctgga ccatcgtatc ggccactttt ctgaagccgg tgatgtttgc acccttcaca
      301 tagttgatgt atccatccgg ttgcgtccct tccttgacgc aggcgttgtg gattttcaac
      361 atgatttcct taagcttctc atccacctct tcagcggtcc aggcgatatg catcgcgttc
      421 tgtgtcatct ccaatccgct gacggcgact ccgccggcgt tggccgcctt gccgggggcg
      481 aagaggatct tcttttcaag gaaatagtac atcgcatccg cctgacaccc catgtttgaa
      541 atctcgacga ctccctgcac cccgttggca acaagggctt tcgcatcggc cagactgagc
      601 tcattctgaa tcgcgcaggg gaacgcaaaa tcgactttaa cctcccatgg ctttttccgg
      661 ggaacgaaca cggcgccgaa ctcttttgca tacggctcga ccacatcgtt gttggaaaga
      721 cgaaggtagg acatatacgc ccatttctcc ggagttccca ctccctcttc atcgtagatg
      781 tacccgtccg ggccgctgat cgtaaccact ttcgctccga ggtgggtcgc tttctgagcg
      841 gctccccagg cgacgtttcc gaatccggaa agggcgacgg ttttccctgt caggctgtct
      901 cctctgagag ccagcattga attggcgaag tacatcgctc cgaacccggt ggcttcaggc
      961 cggaggatcg atccgccgta gttcaggccc ttgccggtga ggactccggt gttctcgtcg
     1021 cgcaggcgct tgtactgccc gtagagatat ccgatctccc gagctcccac gccgatgtcc
     1081 ccggcgggaa cgtcggtatc gggcccgatg tacttttgga gctcgctcat gtaggagcgg
     1141 cagaaccgca tgatctcccc gtcgcttttc cctttggggc tgaagtcgct tccccctttt
     1201 cccccaccca tcggcagcgt cgtcagactg tttttaaggg tctgctcgaa ggcgaggaat
     1261 ttaagggttc cgagggtgac gcttgcatga aagcgcagtc ctcccttata ggggccgagg
     1321 gcgttgttga actgaacccg gtatccgcgg ttcacttgaa gattcccctc atcatcctcc
     1381 cattcgactc ggaacgagaa gatgcggtcc gcctcggtca ggcgctcgag gattcgatgc
     1441 ttttcataga cgggattctc attcaccgcg tcgatgatcg actcaagcac ttcacgggtc
     1501 gcttggatga actccggttg ggcggggttc ctgcgttcga gctcattcat aaaatctgaa
     1561 aggttataca tacatctcgc t
//
LOCUS       DAOPUZ010000340         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_88571_length_1578_cov_3.342745, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000340 DAOPUZ010000000
VERSION     DAOPUZ010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88571_length_1578_cov_3.342745"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PKN79_05790"
     CDS             complement(<1..653)
                     /locus_tag="PKN79_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNZ95307.1"
                     /translation="MTIAPFLHMLPILVVILSGYVMGRLLPIDSKTLATVAADFFMPA
                     LLFISMATTDLEGSLVSSLIGSSISITAMLLVVAFIWAKLAGQEPSKTVPSLVFMNSG
                     FLAIPLMKLYGGNLGMNMSVIFDFTQSFAMFTVGVFIITGGLKASSVKAAFASPIIWA
                     TVSGLLVRTLAIPIPETIRQIFLYAGDGAPALASFALGVSLKDFKLTLKVELISALFV
                     "
     gene            complement(739..>1578)
                     /locus_tag="PKN79_05795"
     CDS             complement(739..>1578)
                     /locus_tag="PKN79_05795"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNZ95308.1"
                     /translation="VETVSGESIRDTYADLIYTDESQQTLRYFEPAVITASEQKEAEA
                     VIRLFAKWLPTQNNLIHYRLEEYYKGVERTLVIIKPDNWRYASSRPGMIIDMFSRSGL
                     RIVAIKVLKMSVAQAVRFYGPTKEGLKKRLAPIYGMQARELLEREFNIPLTEELEKTL
                     TESFGDMYGEEQFERIIEFMAGIKTYERPEEEWEEPGLVKSMILVYEGKDAISKIRTI
                     LGATDPTKAAAGTIRREFGSNICINAAHASDSVESAVREMGILEVEKNHLGGVLQHYV
                     AHR"
BASE COUNT          360 a          417 c          441 g          360 t
ORIGIN      
        1 acgaacaatg cgctgatcaa ctcgactttc aaggtgagct tgaagtcttt cagggagacg
       61 ccaagggcga agcttgcaag agccggggcg ccatcgccgg catagaggaa gatctgtcgg
      121 attgtctcgg gtatcggaat ggcgagggtg cgaaccagaa ggcccgagac ggtcgcccag
      181 atgatcgggg aggcaaacgc cgccttgacc gaagaagcct tcaatccgcc ggtgatgatg
      241 aacaccccga ccgtgaacat cgcaaacgat tgagtgaagt cgaagatcac gctcatattc
      301 atcccaaggt ttccaccata gagcttcatc agggggattg caagaaagcc ggagttcatg
      361 aagaccaggg aaggaacggt tttcgagggc tcctgcccgg cgagcttcgc ccagatgaac
      421 gcaacgacca agagcattgc ggtgatggag atcgatgagc cgatcaggga tgaaacgagt
      481 gaaccttcca gatccgtcgt cgccatcgat ataaacagaa gggcgggcat gaagaaatcc
      541 gcagccacgg tggcaagcgt ttttgaatcg atcggcagca agcgacccat cacataccct
      601 gagaggatca cgacgaggat gggaagcatg tggaggaatg gcgctatggt catgatccta
      661 ctgtacttgg tttcctatac gatgaacaag tggagaacgg cttgactgat gatgtgggcg
      721 catttgaacg gggataactc agcgatgtgc cacgtagtgt tggagaactc ccccgaggtg
      781 gtttttttcc acttcgagga ttcccatctc cctgacggcg ctctcgacgg agtcggaagc
      841 gtgggccgca ttgatgcaga tatttgaacc gaactcccgt cggatcgttc cggcggctgc
      901 tttggtcggg tcggtcgctc ccaagatcgt tcgaatcttg ctgatcgcat cctttccttc
      961 gtagacgaga atcattgatt ttaccaagcc cggttcttcc cattcctctt caggccgctc
     1021 atacgtcttg attccggcca tgaactcgat aatccgttca aactgctctt caccgtacat
     1081 gtcgccgaat gattccgtca gggtcttctc aagctcttcc gtcaggggaa tattgaactc
     1141 ccgctcaaga agctcgcgag cctgcatccc gtagatcggg gcgagtcgct ttttgagtcc
     1201 ttctttggtc ggcccgtaaa accggaccgc ctgggcgacg ctcatcttca agaccttgat
     1261 tgcgacaatt ctcaatccgc tgcggctgaa catgtcgatg atcatgcccg gtcgcgagga
     1321 agcgtaccgc cagttatccg gcttgatgat caccaaggtt cgctcaacgc ctttgtagta
     1381 ctcctctaag cgatagtgaa tcaaattgtt ttgggtcgga agccatttgg caaacagccg
     1441 gatgaccgct tccgcttctt tttgctcact ggcggtgatg accgccggct cgaagtaacg
     1501 gagcgtctgt tggctttcgt cggtatagat cagatccgca taggtgtccc ggatcgattc
     1561 acctgacacg gtttcaac
//
LOCUS       DAOPUZ010000341         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_89033_length_1573_cov_2.335968, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000341 DAOPUZ010000000
VERSION     DAOPUZ010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89033_length_1573_cov_2.335968"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1573)
                     /locus_tag="PKN79_05800"
     CDS             complement(59..1573)
                     /locus_tag="PKN79_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HNZ95309.1"
                     /translation="MKTIGFDNERYLEEQRHYILERVKESDGKLYLECGGKLLFDYHA
                     SRVLPGFDPNVKMRVFQSLADEIDVIICIHSGDIERRKMRSDFGITYDADVFKMIDDF
                     AKWGLAVNKVVITRYDGEAKSLQFKQVLEQRGITVYTHSYTRGYPTDIDTIVSDEGYG
                     ANSYIETTAPVVLVTGPGPGSGKLGTCLSQMYHDYKMGRKSRYSKFETFPIWNLGIDH
                     PVNIAYESATADLGDKILIDHFHASAYGTLAVSYSRDLEAFPLLRRILLRIMGEEPYK
                     SPTQMGVNRCGFGIIDDEVVRKAGEQEIIRRYYRSSCEYVQGIATKETADRSRAIMEE
                     AKLDPNQRVVVPAAQEALRRGIERGKGVNRIVCAAALELPTGEIATGCNSPLLHASSA
                     LILNAVKILAGIDHEVDLIPPAIVQSVTAMKRDVLKGRGVSLNLDETLICLAMSRAIN
                     EDARKASEELPRLMGCEVHMTHIPSSGDSSGLRKLLLNVTSDPRFPTSNLYNPA"
BASE COUNT          305 a          468 c          429 g          371 t
ORIGIN      
        1 caccctatga cagggtcaag tatttgacgg tttcgaggtg ttttacatcc ttgatccgct
       61 atgcggggtt gtacagattc gaggtcggaa agcgcggatc gctcgtcacg ttcaacagga
      121 gctttctcag tcccgaagaa tcgccggatg agggaatgtg ggtcatatgg acttcacagc
      181 ccatgagccg tggcagctcc tcgctcgcct tccgggcatc ttcattgatc gcccgactca
      241 tcgccagaca gatcagcgtc tcatcgagat tcagactgac tcccctgcct ttgagaacat
      301 cccgcttcat cgcggtgacg gattgaacga tcgccggcgg aatcagatca acttcatgat
      361 cgataccggc aaggatcttc accgcgttga gaatcaacgc gcttgaagcg tggagcagcg
      421 gggagttgca tcccgtggcg atctccccgg tgggcagctc caacgcggcg gcgcacacga
      481 tgcggttcac ccctttgccc cgctcgattc cccgcctcag ggcttcttgc gcggcgggta
      541 cgacgacccg ctggttggga tccaacttgg cctcttccat gatcgcccgg cttcgatcgg
      601 cggtctcttt tgtcgcgatc ccctgaacat actcacagct gcttcgataa tagcggcgga
      661 tgatctcctg ctctcccgct ttgcggacca cctcgtcatc aatgatcccg aaaccgcacc
      721 ggttcactcc catctgcgtt ggactcttat acggctcttc acccatgatc cttaggagaa
      781 ttcttctgag cagcgggaac gcctccagat cccggctgta actgaccgcg agcgttccgt
      841 atgccgaagc gtggaagtgg tcgatcagaa tcttgtcccc cagatccgcc gtcgcgcttt
      901 cataggcgat attcaccgga tgatcgattc cgaggttcca aatgggaaac gtttcaaact
      961 tcgaatagcg gctctttctc cccatcttgt aatcgtggta catctggctg aggcaggtgc
     1021 ccagtttccc cgatcccggg ccgggaccgg tgaccaacac cacgggagcc gtcgtctcga
     1081 tataggagtt tgctccatat ccttcatcgc ttacgatcgt atcgatgtcc gtgggatagc
     1141 cgcgggtgta ggagtgggta tagacggtga tcccccgttg ctcgagaacc tgtttgaact
     1201 ggagggattt cgcttcgccg tcataccggg tgatcaccac cttgttgacg gcaaggcccc
     1261 acttggcgaa atcgtcgatc atcttgaaca cgtcggcgtc ataggtgatt ccgaagtcgc
     1321 tgcgcatctt gcgccgttcg atatcgccgc tgtggatgca gatgatcaca tcgatctcat
     1381 cggcaagcga ttggaagacc cgcatcttca cattgggatc gaagccggga agaacgcggg
     1441 aggcgtggta gtcgaagagc agcttgcccc cgcactccag atagagcttg ccgtcgcttt
     1501 ccttgacccg ctcgaggata tagtggcgct gctcctccag ataccgttca ttgtcgaagc
     1561 cgatggtttt cac
//
LOCUS       DAOPUZ010000342         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_89735_length_1565_cov_3.185430, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000342 DAOPUZ010000000
VERSION     DAOPUZ010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89735_length_1565_cov_3.185430"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PKN79_05805"
     CDS             complement(<1..1116)
                     /locus_tag="PKN79_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNZ95310.1"
                     /translation="MKLAFEYGHGLMEANLPDTTDVFIPGETVKDPPVLEDIVGATRA
                     ALENPLGMKPLSRLVTRDSKIVIIFPDKVKGGFQADSHRKVSIPLIIEQCLAAGADER
                     NIKLICSNGLHRKNTDREIRSILGDAVFDRFHPWGQIVNHDSEDWDNLIDLGHEPHYN
                     CPVIMNREVFESDVAILIGHALGNPYGGYSGGYKHCATGLTHWKSIGSHHNPATMHKQ
                     DFVPVNSHSEMRGKFDAIGRYMEERMGKPYFVCDAVLDTNSRQIGVFAGTAEEVQKAS
                     WALADKRTYVPWAKKKYDVMVFGMPQEFHYGNGMGTNPIFILQAISAQIIRHKRVLVD
                     NPVIICSSLCNGYFHDEEFPSYRETFELFQKDYANTLP"
     gene            complement(1113..>1565)
                     /locus_tag="PKN79_05810"
     CDS             complement(1113..>1565)
                     /locus_tag="PKN79_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNZ95311.1"
                     /translation="ELFGALHRILNEKVLAAQKAGLNVLYCIGENADEVNIRTSLLQN
                     QIEAGLAGTDQSLVTLAYEPIWAIGPGKTPPTAEEIASIAKEIKSFTDMELVYGGGLK
                     EENAEAIGSIGSLAGGLVALTRFSGEIGFYPDEFATIVENYTRGRNMS"
BASE COUNT          311 a          434 c          436 g          384 t
ORIGIN      
        1 gggaagggtg ttggcataat ccttttggaa aagttcaaag gtctcgcgat aggagggaaa
       61 ctcctcatca tggaagtagc cgttgcagag cgaggaacag atgatgaccg ggttgtccac
      121 cagaacgcgt ttgtgtcgga tgatctgggc gctgatcgcc tggaggatga agatcgggtt
      181 ggtgcccatg ccgtttccgt agtggaactc ctgcggcatc ccgaacacca taacatcgta
      241 cttctttttc gcccaaggca cgtaggttct cttgtcggca agcgcccagg atgccttctg
      301 aacctcttcc gccgttccgg cgaacacccc gatctggcgg ctgttggtat caaggaccgc
      361 atcacacacg aaatagggct tacccatccg ctcttccatg taccggccga tcgcgtcaaa
      421 cttgccgcgc atttccgaat gactgttcac cggcacgaag tcctgtttgt gcatcgtcgc
      481 cggattgtgg tggcttccga tcgatttcca gtgggtgagg ccggtcgcac agtgcttgta
      541 gccgccgctg tagccgccgt aggggtttcc gagggcgtga ccgatcaaaa tggcgacgtc
      601 cgattcgaag acttcccggt tcatgatcac cgggcagttg tagtgcggct catgcccaag
      661 atcgatcagg ttgtcccaat cctcgctgtc gtggttgacg atctgtcccc aaggatgaaa
      721 gcggtcgaag accgcatcgc caaggatcga cctgatctcc cgatcggtgt ttttccggtg
      781 aaggccgttt gagcagatca gtttgatgtt tcgttcatcg gctccggcgg ccaggcattg
      841 ttcgatgatc aggggaatcg agacctttcg atgggaatcc gcttggaagc cccctttcac
      901 tttatcggga aaaatgatga cgatctttga atccctcgtt accagccgac tcaacggctt
      961 cattcccagg ggattctcca atgcggctct ggtcgccccg acgatgtcct ccagtacggg
     1021 aggatccttc accgtttcac cgggaataaa gacgtcggtg gtatccggca gattcgcttc
     1081 catcagccca tggccgtatt caaacgctaa tttcatgaca tgttccttcc tcgtgtatag
     1141 ttttcaacga tagttgcaaa ttcatcgggg taaaatccga tctcgccgct gaaacgggtc
     1201 agagcgacaa ggcccccggc caaggaaccg atcgatccga tcgcctccgc attctcttcc
     1261 ttcaggccgc cgccatagac gagctccata tccgtgaacg atttgatctc ttttgcgatg
     1321 gaagcgatct cttcagcggt gggcggggtc tttcccggtc cgatcgccca gatgggttca
     1381 taggcgaggg tgaccagcga ttgatcggtt ccggcgagac cggcttcaat ctggttttgc
     1441 aggaggcttg ttcgaatgtt gacttcatcg gcgttctcgc cgatgcagta cagcacgttc
     1501 agccccgctt tttgcgcagc gaggactttt tcattgagaa tccggtggag agctccgaac
     1561 agctc
//
LOCUS       DAOPUZ010000343         1563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_89954_length_1563_cov_2.688992, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000343 DAOPUZ010000000
VERSION     DAOPUZ010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89954_length_1563_cov_2.688992"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1563)
                     /locus_tag="PKN79_05815"
                     /pseudo
     CDS             complement(1..1563)
                     /locus_tag="PKN79_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606990.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
BASE COUNT          353 a          427 c          411 g          372 t
ORIGIN      
        1 tcccggtaca ccggataatc gcaaaggtgg atcgactcgg gcagctcgtt ggaacggagg
       61 ttctgataga tctcttcaac ggtgaacgga atcaccggag cggcaacctt gatgaacgtc
      121 atcagggcat ggtagagggt atcatagcct gcttcttgtc ctcatcgttc tcccctctcc
      181 agaaccggcg ccgactgcgg cggatatacc agttgttcaa ctcatccagg aacggaatga
      241 aataggaaca tgccttttgg tatcataggc atcgaaggcg gcggtggcgt tttccaccag
      301 ccgggcggtc gcgctgacga tccactgatc catcggattc ttcagatccg catggttcgt
      361 tgcggacggc tcgtaggagt cgatattcgc ataggtgacg aagaatgagt acgcattcca
      421 cagcggaagc agaagacctt taaggacatc cttgaccgcc tcttcgctga acttcaggtc
      481 ttccgcccga acgaccgcgc tgctcatcaa cgcgaagcgg agagcatccg ctccatactg
      541 attgatgagt tccatcgggt cggtgtagtt ccgctcactt ttgctcatct tcttgccttc
      601 ggcggtcagg acgattccgt tggtgatgtt gttgtaaaat gcgggttttt catacaaacc
      661 ggccgccaat acggtaaggg tataaaacca gccgcgggtt tggtccaacc cttcacagat
      721 gaaatcggcg gggaagtgtt tttcaaaata ctcccggttt tcaaaaggat aatgcagctg
      781 tgcatacggc atggacccgg attcaaacca acagtctaag acatcgggaa tcctccgcac
      841 cgtcgatttg ccgtcgggac tcggccaggt aagatcgtcc acgaagtgct tgtgcagatc
      901 ggtgactttc tgaccgcact tcgtctccag ctcttccaaa ctgccgatca cctcaatgtg
      961 atttccatca tcgctcttcc agaccgggat cggattgccc cagaagcggt tgcggctgat
     1021 cgaccaatcg cgggcgccct caagccactt gccgaagcgg ccgtacttaa ggtgttccgg
     1081 catccagtag atctgctcgt tcgctgcaag catgaacggc ttgatctgat cgatatcgac
     1141 gaaccagctg ctcatcgccc gatagatcag cggcatcttg gttcggtaac agaacggata
     1201 cgggtgaacg atcgtttctt cgaggaagag cagattatgc tcctttaaat actcgatgat
     1261 gtcgtcatcg caatccttga cgaaccgccc cgcatagtcg gaaacctctt cggtgaaatt
     1321 gcactccatg tcgatcgggc agatcgtggg gattccggtt cccttcaaca cctgatagtc
     1381 atcctcgccg aaacccgggg cggtatgaac gatacccgat ccgtcttcgg tcgtaacgta
     1441 atcgcccaga acggtgacaa acgctccgct cttctctttt tcatcggcga agtacggaaa
     1501 gagcggctcg tagtgcagcc cttcatagaa gaatttgatt ctgtacggaa acgttggtac
     1561 tgg
//
LOCUS       DAOPUZ010000344         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_90785_length_1554_cov_3.104736, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000344 DAOPUZ010000000
VERSION     DAOPUZ010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90785_length_1554_cov_3.104736"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKN79_05820"
     CDS             complement(<1..702)
                     /locus_tag="PKN79_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNZ95312.1"
                     /translation="MKVAIFDSTLRDGSQGEGISFSVEDKLKVVRALDALGIDFIEAG
                     NPGSNPKDQEFFDRARTLELKHAAIVAFGSTRRKGIEAKDDPYLQSLIGAKTKIVSIF
                     GKSWDFHVTEILRTTLKENLAMIEDSIRFCCDAGVEVFFDAEHYYDGYLADREYALET
                     LRAALKGGASTLVLCETRGGIIPSEFERITKETMAAIPNAQWAVHAHDDIGTAVASSI
                     LAVQCGVGQVQGTLLG"
     gene            complement(699..>1554)
                     /locus_tag="PKN79_05825"
     CDS             complement(699..>1554)
                     /locus_tag="PKN79_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNZ95313.1"
                     /translation="TRGDIYPFETNINTEQITQSARVLKQITGVKIHPSKAIVGSNAF
                     AHESGIHQHGMLANSLTYEIMTPESVGVKKTSLVLGKHSGQHAFEQRLGELGYSFTKE
                     EFSRLFNEFKNLADRKKNVEERDLIALVESAHQGSPTIWELDSFVVNSGNLITSTACV
                     TLRKGDEKHQEVAWGTGPVYAALRAVEKIVDHPFSLEDYTLQAVTEHRDALGEVFVKL
                     RDERGTYRGRGVSTDVIEASILSCLAAINKMIDGAPAKREHPFKPTNDHSFENDMLMN
                     NSDKRRFM"
BASE COUNT          288 a          461 c          446 g          359 t
ORIGIN      
        1 tccaaggagc gttccctgaa cctggccgac cccgcattga accgccagga tggaagaagc
       61 caccgccgtt ccgatatcat cgtgggcgtg aaccgcccac tgggcgtttg gaatcgccgc
      121 catcgtctcc ttggtgatcc gctcaaactc ggaaggaatg atcccgcccc gggtctcgca
      181 gagaaccagc gtagaggctc cccccttaag cgccgctctg agggtttcga gggcatactc
      241 cctatcggca agatatccgt cgtagtagtg ctccgcatcg aagaagacct ccacgccggc
      301 atcacaacag aagcggatcg aatcctcgat catcgcaagg ttttccttaa gcgtggtccg
      361 aagaatttcg gtcacgtgga agtcccagct tttgccgaag atcgaaacga tcttcgtctt
      421 tgcgccgatc agggattgga ggtacggatc gtccttcgct tcgatcccct ttcttcgggt
      481 cgaaccgaag gcgacgatcg ccgcatgctt cagctccaag gttcgagcgc ggtcgaagaa
      541 ctcctgatcc ttcggattgg aaccggggtt ccccgcttcg atgaagtcga tccccaaggc
      601 atccagggct cggaccactt tcagcttgtc ttcaacggaa aaactgattc cttcgccttg
      661 gctgccgtcg cggagggtgg agtcaaagat cgctaccttc acatgaatct ccttttatcg
      721 ctgttgttca tgagcatgtc gttttcaaag gaatggtcgt tggtcggctt gaaggggtgc
      781 tcccgcttgg ccggagcgcc gtcgatcatc ttgttgatcg cagcgaggca ggaaaggatc
      841 gatgcttcga tcacatccgt cgacacgccc cggccccggt aggtcccccg ctcatcccga
      901 agcttgacga acacctcgcc gagggcgtcc cgatgctcgg tgaccgcctg cagcgtatag
      961 tcctccaggc tgaacggatg gtcgacgatc ttctccaccg ccctgagggc agcgtagacc
     1021 ggtccggtcc cccaggcgac ctcctgatgt ttttcatccc ccttcctcag cgtgacgcac
     1081 gccgtcgagg tgatgaggtt gccgctgttg acgacgaagg agtcaagctc ccagatcgtc
     1141 ggagaacctt gatgagccga ttcgaccagg gcgatgaggt cgcgctcttc gacgttcttc
     1201 ttccggtcgg ccagattctt gaattcattg aagagccgtg aaaactcctc tttggtgaag
     1261 gagtagccca gttcgccgag gcgttgttcg aaggcgtgct gcccgctgtg cttgccgagc
     1321 accaggctcg tcttcttcac cccgacgctt tcgggagtca tgatctcata ggtcaggctg
     1381 ttggccagca ttccatgctg gtggattccg ctttcgtggg cgaacgcgtt ggaaccgacg
     1441 atcgctttgg aggggtggat cttcaccccg gtgatctgct tcagcactcg ggcggactgg
     1501 gtgatctgct cggtgttgat gttggtttca aagggataga tgtcacccct ggtt
//
LOCUS       DAOPUZ010000345         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_91385_length_1547_cov_3.623995, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000345 DAOPUZ010000000
VERSION     DAOPUZ010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91385_length_1547_cov_3.623995"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKN79_05830"
     CDS             <1..581
                     /locus_tag="PKN79_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95314.1"
                     /translation="KGLAHVPFIYLSFPIDIEVSFAVDTKTATDLLLSAKWRFAEKRG
                     TSFAVGANLQLIEVGDSNTFGVQGYLASTFRHTLMDLPTKSSIMVGYSWKQGTQALSN
                     IDFAVGLETMLFPSKLKNHLALLLDFGNISYSLSPSGGNAQNRGLVNVGLRVPAFKLF
                     PSTTISFDVRALDLFDHAGRAVSFSTALTFRP"
     gene            complement(582..>1547)
                     /gene="pyk"
                     /locus_tag="PKN79_05835"
     CDS             complement(582..>1547)
                     /gene="pyk"
                     /locus_tag="PKN79_05835"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNZ95315.1"
                     /translation="NLPGVPLVHLSSFTEKDKADLAFGFKNKIDYVALSFVRSGDDVA
                     LLKKYMTETFGHAIPIISKIEKPEAVDNIHQIMDESSAIMIARGDLGVEVPAEEVPLI
                     QKSIIQMGINRNLPVITATQMLESMIYRPRPTRAETNDVANAVLDGSSAVMLSGETAA
                     GGYPLQAVRTMANITSMIEQSEVFKREVMNQILGMDMLRKQTVTEAVGLATRDLAISV
                     KASYITCFTHSGSTARLIAKFRPSVPILAFSPNKSVVQYLCLSWGVTPILIDELESVD
                     ELLLFAPEYLKKNGLVKTGDAVVITAGVPVGSSGKTNMIKVVEIE"
BASE COUNT          340 a          449 c          377 g          381 t
ORIGIN      
        1 acaaaggtct tgcccacgtc cccttcatct acctttcctt ccccatagat attgaagtgt
       61 cgttcgctgt tgatacaaaa accgcgaccg atctgcttct ttcagcgaag tggcgttttg
      121 ccgaaaaaag aggcacatca tttgccgtag gagccaacct ccaactgatt gaagtgggcg
      181 actccaatac gtttggtgtt cagggatacc ttgcatccac attccgccac accttgatgg
      241 acctgccgac caagtcatcg atcatggtcg ggtactcctg gaagcagggg actcaggctc
      301 tttcgaacat cgacttcgcc gtcggccttg agacgatgct cttccccagc aagctgaaga
      361 accatttggc tcttcttctc gacttcggga atatctccta cagcctcagc ccctcgggtg
      421 gaaatgccca gaaccgggga ttggtcaacg taggcctccg tgttcccgct ttcaagctct
      481 tcccctcaac gacaatatct ttcgatgttc gagccctcga tctcttcgat catgccggta
      541 gagcggtaag cttcagcact gctctgacat tccgaccgta gtcattcgat ctcaaccact
      601 ttaatcatat tggttttgcc gctggagccc accgggactc cggcggtgat gaccacggcg
      661 tctccggtct tcacaagacc gtttttcttc aagtactccg gggcgaagag aagcagttca
      721 tcgacacttt ccaattcatc gatgagaatc ggggtcaccc cccaactcaa acagaggtac
      781 tgcacaaccg atttattcgg gctgaatgca agaatcggca ccgaaggacg gaacttggcg
      841 atcagacgag cggtcgagcc ggagtgggtg aaacaggtga tgtaggacgc cttcacactg
      901 atcgccagat cgcgggtggc cagccccacc gcttccgtta cggtctgctt gcgcagcata
      961 tccatcccta aaatctggtt catcacctct cgtttgaata cttcgctctg ttcgatcatc
     1021 gaagtgatat tcgccatcgt gcgaaccgcc tgaagcggat acccccctgc tgcagtctcg
     1081 ccgctgagca tcaccgcgct gcttccatcc aatacggcgt tcgccacatc attggtctcg
     1141 gcacgggtcg gtcgggggcg ataaatcatc gattccaaca tctgcgtagc ggtaatcacc
     1201 gggaggtttc gattgatccc catctgaatg attgattttt ggatcagggg aacctcttca
     1261 gcgggaactt cgacaccaag gtccccccgg gcgatcatga tcgcgctgct ttcgtccatg
     1321 atttggtgaa tattatcgac cgcttccggt ttctcgatct tgctgatgat gggaattgca
     1381 tgcccgaacg tctccgtcat gtatttcttc aacagcgcca cgtcgtcacc gcttcggaca
     1441 aaggaaagag cgacatagtc gatcttgttt ttaaaaccga acgccaaatc ggctttgtcc
     1501 ttttccgtga aggaggacaa atgaacaagg ggcaccccgg gaaggtt
//
LOCUS       DAOPUZ010000346         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_91722_length_1543_cov_3.661290, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000346 DAOPUZ010000000
VERSION     DAOPUZ010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91722_length_1543_cov_3.661290"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKN79_05840"
     CDS             complement(<1..291)
                     /locus_tag="PKN79_05840"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HNZ95316.1"
                     /translation="MDFLVSTSADGTRVLLWPNLAQLWIWFVMYSVIGWLWEVVLSFL
                     QGRGFVNRGFLNGPYCPVYGTGALLVVHLLGGLTNPLLIFLAAAIITTTVEYL"
     gene            complement(381..1253)
                     /locus_tag="PKN79_05845"
     CDS             complement(381..1253)
                     /locus_tag="PKN79_05845"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNZ95317.1"
                     /translation="MRTSSKHWISIITMAASAAIVLLILNSCTTVAIKEEVDMGKTKA
                     VAHRGGALLAPENTLEAFQAGIASGADQLELDIHLSKDDVIIVMHDPTLQRTASQSGA
                     IRDYTAAELKQFNAASAHPGHPPLEIPTLKEVLDLVKDTDLELQIEIKVDKDGNGYEG
                     IEERLIALLKEYELIDRSIVISFDFPTLARIQELQPSLRTGALVSRAYMSKIGSKGPK
                     AVAEGIKALQVNYIGINHTYLSPALHQELRNQELGIGAWTVNDEEAMKKIAALGVDFI
                     TSDRPDLLVATLRP"
     gene            complement(1237..>1543)
                     /locus_tag="PKN79_05850"
     CDS             complement(1237..>1543)
                     /locus_tag="PKN79_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95318.1"
                     /translation="KFFTSPESQAYLAAMTGYMPSRISSAKVPSLVEYYNKNPHWQVA
                     IDQLKYVQPQASVISLPKGTEILRQMVEKLLIGNQDVRKVMAETEADLIKEYNENFK"
BASE COUNT          317 a          417 c          454 g          355 t
ORIGIN      
        1 caggtactcg accgtcgtgg tgatgatcgc cgcggcgagg aagatcagaa gcgggttggt
       61 gagacctccc aagagatgca cgacgaggag cgctccggtt ccgtagaccg gacagtaggg
      121 gccgttgaga aagccccggt tcacgaaccc tcttccctga aggaaggaaa ggacgacctc
      181 ccagagccag ccgatgacgg agtacatcac aaaccagatc cagagctggg cgaggttcgg
      241 ccagagaagg acgcgtgttc cgtccgccga tgtggagaca agaaagtcca tatctccaat
      301 atcggcatga tttgtttttt cggaaagtgt ttgggcacaa aaaaccggag agaaccccct
      361 ccggttttat ggcagggatg ctagggcctg agtgtcgcta ccaagaggtc gggtcggtcg
      421 ctggtgatga agtccactcc gagggcggcg atcttcttca tcgcttcctc atcgttcacc
      481 gtccaggcac cgatacccag ttcttgattc ctcagctcct gatgcagagc aggggaaaga
      541 taggtatggt tgatgccgat gtagttcacc tgaagcgctt tgattccttc agcgacggct
      601 ttgggacctt ttgaaccgat cttgctcata tacgccctgc tgacgagcgc accggttctg
      661 aggctcggtt ggagctcttg gatcctggcg agcgtcggaa aatcgaagga gatgacgatc
      721 gaacggtcga taagctcata ctccttaaga agcgcgatga ggcgctcttc gattccctca
      781 tatccgtttc catccttgtc gaccttgatc tcgatctgaa gctcaaggtc ggtatccttc
      841 acgagatcca agacctcctt cagggtcgga atctcaagcg gaggatgccc cggatgagcg
      901 cttgccgcat tgaattgctt caattccgct gcggtgtagt cgcggatcgc tccgctttgg
      961 cttgcggttc gctggagggt tggatcgtgc atcacgatga tcacgtcatc ctttgagaga
     1021 tggatatcca gctcaagctg atccgccccc gaagcgattc ccgcctgaaa cgcttcaagc
     1081 gtgttttccg gagccagaag cgctccgccg cgatgggcca ccgccttggt tttccccata
     1141 tcgacctcct ccttgatggc gaccgtggtg cagctgttga gaatcaagag gacgatggcc
     1201 gcggatgcgg ccatcgtgat gatcgaaatc caatgcttac ttgaagttct cattgtattc
     1261 cttgatcagg tcggcttcgg tctcggccat gaccttgcgg acatcctgat tgccgatcag
     1321 gagcttttcg accatctggc ggagaatctc ggtgcccttg ggcagggaga tgacgctcgc
     1381 ctggggctgg acgtatttca gctgatcgat cgccacctgc cagtgcgggt tcttgttgta
     1441 gtactcgacc aggctgggaa ccttcgccga agaaatgcgc gagggcatgt agccggtcat
     1501 cgcggcgaga taggcctggc tttcggggct cgtaaagaac ttc
//
LOCUS       DAOPUZ010000347         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_92664_length_1533_cov_2.937754, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000347 DAOPUZ010000000
VERSION     DAOPUZ010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92664_length_1533_cov_2.937754"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PKN79_05855"
     CDS             complement(<1..538)
                     /locus_tag="PKN79_05855"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNZ95319.1"
                     /translation="MDFLLGGILATTWKDLVMYCVGGILIYLAIRKKLEPSLLLPMGF
                     GAILVNLPFSGAVTQLVEGIGPVQGILDWLFSVGIESAEMMPLLLFIGIGAMIDFGPL
                     LSNPKLILFGVAAQWGIFATISVATLMGFSLADAASIGIIGAADGPTSILVSQVLKSG
                     YIGPIAIAAYSYMALVPII"
     gene            complement(649..>1533)
                     /locus_tag="PKN79_05860"
     CDS             complement(649..>1533)
                     /locus_tag="PKN79_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNZ95320.1"
                     /translation="GVYISPSISIAALGMVESLLCGTYAEKMKGEKFHATRELYAQGI
                     GNMIIPLFGGIPATAAIARTSVAIRAGQQTRLTSIFHSVALIASVFFLAPFLSRVPLA
                     ALAGVLLVTAWRMNDWKSIKQIFSKRMKTSIAQFSITLVATVVFDLTTAILLGIGFSI
                     VMFVIKSNKIMIEIDPVTNRLGKKAKPTKVVYVDGALFFGSQGKLTESIAPLIESGVK
                     RILLSLRGVPNIDHSSITELAEIVRLARKKEVELLFCGVQPRVESMLRRLDFYSLLPE
                     ERFYPSVVVALETIAKEV"
BASE COUNT          365 a          455 c          432 g          281 t
ORIGIN      
        1 ggatgatcgg aaccagcgcc atgtaggaat acgccgcgat cgcgatcgga ccgatatacc
       61 cggacttgag cacctgcgag acgagaatcg aggtcggtcc gtccgccgcc ccgatgatcc
      121 cgatcgaagc ggcatccgcc agagagaagc ccatcagcgt cgccacgctg atcgtggcga
      181 agattcccca ctgcgccgcc actccgaaga gaatcagctt ggggttcgac aacagcggtc
      241 cgaagtcgat catcgccccg attcctataa agaggaggag cggcatcatc tcggccgact
      301 cgatgccgac ggagaagagc caatcaagga tcccctgcac cggaccgatc ccttcgacaa
      361 gctgcgtcac cgccccgctg aagggaaggt ttacgagaat cgctccgaag cccatcggca
      421 gaagcagcga gggttccagt tttttcctga tggccaaata gatgaggatt cccccaacac
      481 agtacatgac cagatctttc cacgtcgtgg ccagaatacc gcccaataag aagtccataa
      541 agacctccgg ggtaataaaa ataggggaag aagttgactt cgcccaacgg acaggaagct
      601 tccccgacga gatcagtgta atggtctatg cagttctagt caaacagcct acacctcttt
      661 tgcgatcgtc tccagggcga cgaccaccga cgggtagaag cgttcttcag gcagcaggga
      721 atagaagtcc agtcgcctta acatcgactc cacccgcggc tgaaccccgc agaagagcag
      781 ctcgacctct ttcttccggg cgagccggac gatctcggcc agctcggtga tcgagctgtg
      841 atcgatattg ggaacccccc gaagggagag aaggatccgc ttgaccccgc tctcaatcag
      901 cggagcgatc gattcggtga gcttgccttg cgagccgaag aagagcgcac catccacgta
      961 caccaccttg gttggtttcg ctttcttgcc aagccggttc gtaaccggat cgatttcgat
     1021 catgatcttg ttgctcttga tgacgaacat cacgattgaa aaaccgattc caagaaggat
     1081 cgcggtggtg agatcgaaga cgaccgtggc gaccagggtg atggagaatt gggcgatcga
     1141 ggtcttcatc cgcttggaga agatctgctt gatcgatttc cagtcattca tccgccatgc
     1201 ggtcaccaaa agcacgcccg ccaacgcggc aagcggaacc cggcttaaga acggagccag
     1261 aaagaacacg cttgcgatca gggcgaccga gtggaagatg ctggtaagcc gggtctgctg
     1321 gcccgcccgg atcgccaccg acgtacgggc gatcgcggcg gtggcgggaa tgcctccgaa
     1381 aagcgggatg atcatgtttc cgatcccctg cgcatacagc tcccgcgtcg catggaactt
     1441 ctcgcccttc atcttctcgg cgtacgttcc gcacagaagg ctttcgacca ttccgagcgc
     1501 ggcgatcgaa atcgacggcg agatgtagac ccc
//
LOCUS       DAOPUZ010000348         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_93026_length_1529_cov_4.030529, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000348 DAOPUZ010000000
VERSION     DAOPUZ010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93026_length_1529_cov_4.030529"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1339
                     /gene="ffh"
                     /locus_tag="PKN79_05865"
     CDS             5..1339
                     /gene="ffh"
                     /locus_tag="PKN79_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNZ95321.1"
                     /translation="MFDSISNAFSSIMRSIAGKSKISEKNIQDAVEEIKVALLDADVN
                     IRVVRRFINSTMEEALGEKVLKAVDPGDQFVKIVYDKMVNLLGDEDNQKLNLKGPDTP
                     SSILMMGLQGSGKTTTAAKLALRLKKEGRRVLLVAADLVRPAAILQLQVLGKTVGVEV
                     FSIEGEKDPAKVAKAAFAKAKKEQFNTLIVDTSGRMHLDDVLMAEIKKVRDALKPEET
                     LFVADAMTGQQAVTIAKEFEEQVGISGVILTKFDSDTRGGAALSLRSVVGKPIKFIGV
                     GEKVEDLEPFYADRYASRILGMGDIVSLVEKAQEVFDEEQAIKMQQKMAKNTFDLQDY
                     LEQLESMNKMGGLESMLELIPGMKGQVSEDDIDKQEIEREKAIIRSMTYAERENYLIL
                     GPTRRRRVAKGSGTHISDVNRLIKKFEKSRQMMRKLTRNKKYQAEMLKQMGL"
     gene            1369..>1529
                     /gene="rpsP"
                     /locus_tag="PKN79_05870"
     CDS             1369..>1529
                     /gene="rpsP"
                     /locus_tag="PKN79_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNZ95322.1"
                     /translation="MVSMRLKRFGSKKRPDYRIVVIDSREKRQGRAIEEVGHYHPLAE
                     KKEEQLVIK"
BASE COUNT          424 a          337 c          452 g          316 t
ORIGIN      
        1 gacaatgttt gattcgataa gcaatgcgtt cagctcgatc atgcgatcga tcgccggaaa
       61 atccaaaatc agtgaaaaga acatccaaga cgccgttgaa gagatcaagg tggcgctgtt
      121 ggatgcggac gtcaatatcc gcgtcgtccg caggttcatc aattccacga tggaggaagc
      181 gcttggtgaa aaggtcctca aggcggtcga tccgggggac cagtttgtaa agatcgtcta
      241 cgacaagatg gtgaacctcc tcggggacga ggataaccag aagctcaatc tcaaaggtcc
      301 cgataccccg tcttcaatcc tcatgatggg tcttcagggt tccggtaaaa ccactaccgc
      361 tgcgaaactt gcccttcgat tgaagaaaga gggccgaaga gtccttctgg tcgccgccga
      421 cctggtccgg cccgctgcta ttctgcagct tcaagtcctt ggaaagacgg tcggtgtcga
      481 ggtcttttcc atcgaagggg aaaaggaccc ggcgaaggtt gccaaagcag cgtttgcaaa
      541 agcgaagaaa gagcagttca ataccttgat cgtcgatacc agcggccgga tgcacctcga
      601 tgatgtgctg atggcggaga tcaaaaaggt ccgggatgcg ctcaagcccg aggaaacgct
      661 gttcgtcgcc gatgcgatga ccggtcagca ggcggttacg atcgccaagg agtttgaaga
      721 gcaggtcggc atcagcggag tgatccttac gaagtttgac agcgataccc gcggaggggc
      781 cgctctttca ttgcgttcgg ttgtcggaaa gccgattaaa ttcatcggag taggcgagaa
      841 ggtcgaagac cttgagccgt tctatgccga tcggtacgcc tcccgaattc tcggaatggg
      901 ggacatcgtc tcgcttgtcg agaaagctca ggaagtgttc gatgaagagc aggcgatcaa
      961 gatgcagcag aagatggcga agaacacctt cgatcttcag gattatcttg agcagctgga
     1021 atcgatgaac aagatgggcg gtctcgaatc gatgttggaa ttgattccgg ggatgaaagg
     1081 tcaagtgagt gaagatgaca tcgacaagca ggaaattgaa cgggagaaag cgatcatccg
     1141 ttcgatgacc tatgctgaac gggaaaatta ccttatactt ggaccaaccc gtcggcgaag
     1201 ggtcgccaag ggaagcggta cgcatatcag cgatgtgaat cgtctgatca aaaagtttga
     1261 aaagtcacgc cagatgatga ggaagttgac gcgaaacaaa aaataccagg ctgaaatgtt
     1321 aaaacagatg ggtctgtagt tgttgtatat catggaggaa ttgtcacgat ggtgagcatg
     1381 agactgaaga ggtttggatc aaagaaacga ccggattatc gaattgtggt gatcgactcc
     1441 cgcgagaagc gccaaggccg cgcaatcgag gaagttggac actatcatcc ccttgcagag
     1501 aagaaagaag agcagttggt gatcaagga
//
LOCUS       DAOPUZ010000349         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_94058_length_1519_cov_3.591530, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000349 DAOPUZ010000000
VERSION     DAOPUZ010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94058_length_1519_cov_3.591530"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PKN79_05875"
     CDS             <1..736
                     /locus_tag="PKN79_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ95323.1"
                     /translation="VGLTEELKRNPSPTGLSGGQQQRVAIARALVYDPDILLLDEPLA
                     NLDAKLRRYMRAEIRRIQKATNITTIFVTHDQEEAMAIGDRLAVLRKGVIEQIGTSDE
                     LYTNPSNAFVANFIGKMNFFNASLVGSHSAKIDATDETVEVRPERSRLEAGLKKGSEV
                     FVGARPEQLEIVEEASDQTIAATVQIIQHLGQVVRYEVLLDKAISPATMEIDMPHYLS
                     NVREQDSVFIRFKEGMPFLFNREEQN"
     gene            736..>1519
                     /locus_tag="PKN79_05880"
     CDS             736..>1519
                     /locus_tag="PKN79_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ95324.1"
                     /translation="MSHDPVNAKKMRKDITPYLIFTIPLVFMMIFLMYPMVLTILRAF
                     MPSGNKLQPGAFSLDGFTKFFTSSMYKKALNNSFIVSLSVTGLCILIGVPMGYFVARV
                     KIPGKNFMLSLGILPIIMPSFVGAFTWVILLGRQGVVRHFLNLLLAPLGITIPTIYGM
                     FGMILCMTLTYYPFVFQLAYGAFASANALLEEAGMLMGASRWHIFRTITFPLILPSLG
                     AGALLVFVRAIGNFGIPAIIGGDKYVLPTLIYFRVNGFWDLNG"
BASE COUNT          327 a          402 c          421 g          369 t
ORIGIN      
        1 tgtcggtctg accgaggagt tgaagagaaa cccttcaccg accggccttt ccggcgggca
       61 gcagcaacgc gtggcgatcg cccgggcatt ggtatatgat ccggatattc ttctgcttga
      121 tgaaccgctg gccaacttgg acgcgaagct tcggcgctat atgcgggcgg agattcgacg
      181 gattcaaaaa gcgacgaaca tcacaaccat tttcgtcacc cacgatcagg aagaggcgat
      241 ggcgatcggc gatcgcctcg ctgtgttacg caaaggagtc atcgaacaga ttggcaccag
      301 cgacgagctg tatacgaatc cttcaaacgc ttttgttgcc aacttcatcg gcaagatgaa
      361 cttcttcaac gcttctctgg taggctccca ctcggcgaag atcgatgcca cggacgagac
      421 ggtggaggtg aggcccgaac gatcccggtt ggaagcgggc ttgaaaaagg ggagcgaggt
      481 ctttgtgggg gctcgccccg agcagcttga aatcgtcgaa gaagcaagcg accaaacgat
      541 cgccgcgacg gttcagatca tccagcacct cggacaggtc gttcgatatg aagtgctgtt
      601 ggacaaggcg atcagccctg caacgatgga aatcgatatg ccccattact tgagcaatgt
      661 ccgggaacag gattcggtct ttatccgctt caaggaggga atgcctttcc tcttcaatcg
      721 ggaggaacag aactgatgag tcacgatcct gtcaatgcaa agaagatgcg caaggatata
      781 acgccgtacc tgatattcac cattcccttg gtgttcatga tgattttcct catgtatccg
      841 atggttttga cgattctgcg ggcgttcatg ccctcgggaa acaaactgca gccgggtgct
      901 ttttcgctcg acgggtttac gaagttcttc accagttcaa tgtacaaaaa agcgttgaac
      961 aactcgttca tcgtcagcct ctcggtaacg gggctctgca ttctcatcgg agttcccatg
     1021 gggtactttg tggctcgggt aaagattccc ggtaagaatt tcatgctcag tctgggaatc
     1081 cttcccatca tcatgccttc cttcgtcgga gcgttcacgt gggtcatcct gctcgggcgc
     1141 cagggagtgg tccgccactt cctgaatctt cttcttgctc cgcttggaat cacgatcccg
     1201 acgatctatg gaatgttcgg gatgatattg tgcatgacgc tgacctacta tccctttgtc
     1261 ttccagctcg cctacggggc gttcgcctcg gcgaatgccc tgcttgagga ggcggggatg
     1321 ctgatggggg catctcgctg gcatatcttt agaacgatca ccttccccct gattcttccg
     1381 agccttggag ctggagctct gctcgtgttt gtccgggcga tcggaaactt cggaattccc
     1441 gcgatcatcg gtggcgacaa gtatgtgctt ccgaccttga tctacttcag agtcaacggg
     1501 ttctgggatc tcaacgggg
//
LOCUS       DAOPUZ010000350         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_94151_length_1518_cov_3.639781, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000350 DAOPUZ010000000
VERSION     DAOPUZ010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94151_length_1518_cov_3.639781"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PKN79_05885"
     CDS             complement(<1..608)
                     /locus_tag="PKN79_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ95325.1"
                     /translation="MNFDTMIDRRSTLSVKWDPSAITNICSNPDAEPFWVADMDFTVP
                     PEAHAEAKALAEHGIFGYPVATDQKTVFMSFVKARHGMMIDEDELSISQGILSSISLS
                     VELFSKEGDGVVVPLPAYAPFLRIVKLHNRTLIPWPMIYDTDRHVFTLDWASLDTVID
                     RAKLLIFCSPHNPTGIVFSEEELIRVAEICKARDVLIISDEIP"
     gene            complement(605..1393)
                     /locus_tag="PKN79_05890"
     CDS             complement(605..1393)
                     /locus_tag="PKN79_05890"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNZ95326.1"
                     /translation="MNHTILCFSGTGNSYHVANRLRISFENARIVMIPDLMDDSTVEL
                     TEAVGLVFPTYFFLPPATVVEFIETVLPKLDLSPVQYFYVVTTKNLFGGWAHSIAEIL
                     LRDRGVALSYASSVRFPNTYIPLFTAPNIMQIEFYYKHAERKILKIAQEIRKETIRPP
                     FRPPFTRYMLEERMGRRFEKVKKSAQASFVVTSKCDGCEFCYRICPTGNIEMVYGKPI
                     FRLNCTGCLGCYHRCPREAITFNRRVSTGHYPNHEAHFDKEYRE"
BASE COUNT          342 a          370 c          455 g          351 t
ORIGIN      
        1 gggatctcat cactgatgat gaggacatcc cgggctttgc agatttccgc aacccggatg
       61 agctcctctt cgctgaacac gatgccggtc ggattgtgcg gggagcagaa gatgagcagt
      121 ttggcccggt cgatgaccgt gtccaaggat gcccaatcga gggtgaagac gtgtcgatcg
      181 gtgtcatata tcatcggcca ggggatcaac gtccggttgt ggagcttgac gatccgcaga
      241 aacggagcgt aggcgggaag cggtacgacg actccgtccc cctccttgga aaaaagctcc
      301 accgagaggg aaatcgaaga gaggattcct tgggagatcg atagttcatc ctcatcgatc
      361 atcatcccgt gccgggcttt gacgaaggac atgaagaccg tcttttgatc ggtggcgacg
      421 ggatagccga agattccgtg ctcggcaagg gctttcgctt cggcatgagc ctcaggcgga
      481 acggtaaagt ccatgtcggc aacccaaaac ggttcggcgt cggggttgct gcagatgttg
      541 gtgatcgccg aaggatccca tttcaccgat agcgttgaac ggcgatcgat catcgtatca
      601 aagttcattc acgatattcc ttatcaaagt gcgcttcatg gttcgggtaa tggccggtgg
      661 aaacccggcg gttgaaggtg atcgcttccc gggggcagcg atggtagcat cccaggcatc
      721 cggtgcagtt gaggcggaat atcggctttc cgtagaccat ctcgatgttc cctgtgggac
      781 agatccggta gcagaattca catccatcac acttagaggt aaccacgaat gaagcttggg
      841 cgcttttttt gactttttca aagcgccggc ccattctttc ttccagcatg tagcgggtga
      901 acggcggccg gaagggcggc cgaatcgttt cttttcttat ctcctgagcg atcttcagaa
      961 tcttccgttc ggcgtgtttg tagtagaatt cgatctgcat gatattgggt gcggtgaaga
     1021 gggggatgta ggtgttggga aatcgaaccg aagaggcgta actgagggcg actccccggt
     1081 ctcgaaggag gatttccgca attgaatggg cccatccgcc gaagaggttt ttagtggtga
     1141 cgacgtagaa gtactgaacg ggggaaaggt cgagcttggg aagaaccgtt tcgatgaact
     1201 cgacaacggt cgccggaggc aaaaagaaat acgtgggaaa caccaatccg accgcttcgg
     1261 tgagttcaac ggtcgaatcg tccatcagat ccggaatcat gacaatgcgg gcgttttcaa
     1321 aggatattct gagcctgttc gccacgtggt agctgttgcc cgttccgcta aaacagagaa
     1381 tcgtgtggtt cattccgcag cctccggttc aaaggatttc aatcgagcac agatctcgac
     1441 ggtgtcttca tcgtcgatcc ccagattcgt gaccaggcgg acggtatcgc cttcggtgtt
     1501 cgccagcata ccgatctt
//
LOCUS       DAOPUZ010000351         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_94431_length_1515_cov_3.166438, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000351 DAOPUZ010000000
VERSION     DAOPUZ010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94431_length_1515_cov_3.166438"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PKN79_05895"
     CDS             complement(<1..455)
                     /locus_tag="PKN79_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ95327.1"
                     /translation="MPKKIDHEARKERILQTALKVFAREGYRDSNLSLIASECNISRP
                     TIYQYFKDKEDIYYYAVKLVTARMFNRYATYAWSSEQPFLKRLTVICTDIMQEAHDHE
                     GELTSLVDVMLQMKREGTDFNAIILRRTAKLSILFKRLLNQGMKEGEIIL"
     gene            complement(562..978)
                     /locus_tag="PKN79_05900"
     CDS             complement(562..978)
                     /locus_tag="PKN79_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNZ95328.1"
                     /translation="MRYLTRRTYGLSPFDSLFDELLSTWGTTQRRVPAVNITEEENAY
                     VLEAELAGYKQDEVSVQIEKHVLKISSNKETNLEEETEKNLVTERCYRCFERSFRLPE
                     DVDENKISAKFADGLLTLTLPKKPVEKPKPIEVKIK"
     gene            complement(1178..>1515)
                     /locus_tag="PKN79_05905"
     CDS             complement(1178..>1515)
                     /locus_tag="PKN79_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNZ95329.1"
                     /translation="SLILASSPIYTDVWVSMGEEAKMSERIGLLKEYQVTMDLLKASG
                     NPDVLFEHCLPAFHDLNTTIGMEVYKEFGLTAMEVTDDVFRSSHSVVFDQAENRMHTI
                     KAIMVATLG"
BASE COUNT          342 a          407 c          361 g          405 t
ORIGIN      
        1 aggatgatct ccccctcttt catcccttgg ttcaagaggc gcttgaagag gatcgaaagc
       61 tttgcggtcc gccgcagaat gatcgcgttg aaatccgttc cttcgcgctt catctgcaac
      121 atcacgtcca caagactggt cagttcccct tcatggtcgt gcgcttcctg catgatatcg
      181 gtgcagatga ccgtcaggcg cttgaggaag ggctgttcgc tcgaccaggc ataggttgca
      241 tagcggttga acatccgggc ggtgaccagc ttgaccgcat aatagtagat atcctctttg
      301 tctttgaagt attgatagat cgtaggtcgg gagatgttac actcgctggc gatcagggag
      361 aggttggaat cccgataccc ctcacgcgcg aacactttca gagcggtctg taaaattcgc
      421 tccttccgtg cttcgtgatc gatttttttg ggcatacaca ctctcctgtt ttctacacta
      481 tatcaccatg ttgtagaact aaacaagcat ttttggaagc cttgcccctt ggcaaggcct
      541 ccatactgcg tgtactcctt cttatttaat ctttacttcg attggtttcg gtttctcaac
      601 gggcttcttc ggaagggtga gcgtcaagag accgtcagcg aatttggcgc tgatcttatt
      661 ctcatcgaca tcctccggaa gtcggaagga gcgctcgaag caacggtagc agcgctcggt
      721 cacgaggttc ttctccgttt cctcttcaag gttggtctcc ttgttggagc tgatcttcaa
      781 cacatgtttc tcgatttgga ccgaaacctc gtcctgctta tacccggcca gctccgcctc
      841 gagaacatag gcgttctctt cttcggtaat gttgaccgcc ggcactcgtc gctgtgtcgt
      901 tccccaagtg ctcaacagtt catcaaagag tgagtcaaag ggggacaaac catacgttct
      961 tcttgttaaa tatctcatat gtgtacctcc tacatctgta tatttctatc accacatatg
     1021 atgcaacaag catgccaacc cttatattat agtctgtagg tacaaatata tctctataag
     1081 ttaatatata aacgaaagct cgctcctacg cttcaaggga gcgggtcaag gggaggcgat
     1141 ccccaaggag gactgcatta atcgggtcat tatgacccta tccaagggtg gcaaccatga
     1201 tcgccttgat cgtatgcatc cgattctccg cctggtcaaa caccaccgag tgagaggaac
     1261 ggaacacgtc atccgtcact tccatcgccg taaggccgaa ctctttgtag acctccattc
     1321 cgatcgtcgt gttcagatcg tgaaacgccg gaaggcagtg ctcgaagagc acgtcggggt
     1381 ttcccgaagc ctttaaaaga tccatcgtga cctgatactc cttcagcagc ccgatccgct
     1441 cgctcatctt ggcttcctcg cccatcgaga cccatacatc ggtgtagatg ggcgaggaag
     1501 ccaagatgag cgagc
//
LOCUS       DAOPUZ010000352         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_95239_length_1507_cov_3.076446, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000352 DAOPUZ010000000
VERSION     DAOPUZ010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95239_length_1507_cov_3.076446"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..792)
                     /locus_tag="PKN79_05910"
     CDS             complement(118..792)
                     /locus_tag="PKN79_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95330.1"
                     /translation="MIIPMDSNILGLILSYAFLGVVIAFGIFLKNRFGISSESMRKIV
                     HIGVSNWWFLNLYYFTSLGWAVVGPICFIVVNSLFTLFNWGKHIGLDDRKRNWGLVYF
                     PVTLLLLVIGMHQGVFTPLEATIAVMVMGWGDGLAAMVGSKWGRKKIKTLNKSYPGCL
                     TMGIVSFIVTVLALGTGSSLSAGTILFASFLVGAVAMFVEAFTPWGLDNITVPLAVLL
                     VLRFFL"
     gene            999..1364
                     /locus_tag="PKN79_05915"
     CDS             999..1364
                     /locus_tag="PKN79_05915"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HNZ95331.1"
                     /translation="MEHSWQRLLAMTGGKRFLVTEIHIPEEGITIGGSFELPSLARIP
                     IEDQIFIAMFIKTNGSIKQMESIFNISYPTVKNRLNRIAKQLDIGDIEVRTPTRMADL
                     LTRLEEGTITVADALKEIE"
BASE COUNT          420 a          414 c          356 g          317 t
ORIGIN      
        1 gcgcgatgat ccccatgatg ccgacgagaa tccacagcgc tccgtcgagg gtgaaaaacc
       61 atcgatcgaa gagcgaagtg agcgatgaag tcggttgaac caagagagcc cagctactca
      121 taggaagaac ctcaacacaa gcaataccgc caagggaacc gtgatgttgt ccaaccccca
      181 cggggtgaat gcttccacga acatcgccac cgctccgacg aggaacgagg caaaaagaat
      241 cgtgccggcg gaaagagaag aacccgtccc aagagctaaa accgtgacga tgaagctgac
      301 gatccccatc gtcagacatc ccggataaga cttattgagt gtcttgatct tttttcttcc
      361 ccacttcgag ccgaccattg cggcaagtcc gtcgccccat cccatcacca tgacggcgat
      421 cgtcgcttca agaggggtga acaccccctg atgcattccg atcaccagca acaggagcgt
      481 aacggggaaa tagaccaggc cccagtttcg cttgcggtca tccaaaccga tatgctttcc
      541 ccagttgaag agcgtaaaga gggagttcac cacgatgaag cagatcggtc cgacgaccgc
      601 ccaaccgagc gaagtaaagt aatacaggtt caaaaaccac cagttggaaa ccccgatatg
      661 aacgattttc cgcatcgact cgctcgagat gccgaatcga tttttcagga atatgccgaa
      721 ggctatcaca accccgagaa acgcatagga gaggatgagc ccgagaatgt tgctgtccat
      781 agggataatc atgcgcatcc tcttcaacaa tttcaagggg cgcaaagttc aaaaccccaa
      841 tcttccatcc aaacacgccg acatgcaggc aatccatcac gcaccaacca gtaaaactta
      901 attattttaa ttattatttt taattttatt aattatttta atattatata ttgactttat
      961 taattcattt gcatatggtg gaagcaacgg aggaacctat ggaacacagt tggcagcgac
     1021 ttcttgcaat gacgggggga aaaagattcc tggttacgga aatccatatc cccgaagagg
     1081 ggattacgat tgggggatcc tttgaactgc cttcgctcgc acggataccg atcgaggatc
     1141 agatcttcat cgcgatgttc atcaagacga acggctcgat caagcagatg gagtcgatct
     1201 tcaatatcag ttaccccacg gtgaagaacc ggctcaaccg aatcgccaaa cagttggata
     1261 tcggtgacat cgaagtccgg acccccaccc gcatggccga tctccttacc cggctggagg
     1321 aaggcacgat cacggtcgcc gatgcgctca aggagatcga gtgatgccgc cgatgcttat
     1381 gattttcaga ctcaagcgac cggagagaag accgatcagc ctctacatcc ccctgctgct
     1441 tgcatacatc ccgctcctgc cgctcgtcct ccttgcactg gtgagtcttc cctttctggc
     1501 gatttca
//
LOCUS       DAOPUZ010000353         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_95921_length_1500_cov_3.525260, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000353 DAOPUZ010000000
VERSION     DAOPUZ010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95921_length_1500_cov_3.525260"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="PKN79_05920"
     CDS             complement(<1..1179)
                     /locus_tag="PKN79_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ95332.1"
                     /translation="MGIDDRLKRLSRLQGEDPGIFALAVHSFIEGELRRRYDLPYEEE
                     PSFNALSDRLVEEVGEKSRQFLANLKAAHKAVNEVRHEFNALGVDHALMAAAHLYTFC
                     YFIDIDEGALQTLYKIKRELDDTWTRSESRSELITSNQILQTELQKILAEKGSLSDQL
                     SELKEREEEREQVDDRIHSLEHQIRNLLKDKDRNEKKIKELRDERHVLQVQRRSLEKQ
                     TRDLEEYLHVMRTMTSYTRTRRDYEHLILRLSAQQEEALRRIDLSSDFLITGGPGTGK
                     TLVLLKAIGKKSEKSNVALLTYTNSLAKYDQYLTRLLGIEMGQIMTIHHFLRERIEEG
                     ESIKVLAGGDTDILIRLCEPYASLQWTAKDIYDEIVHVLWAFGHTRQRYCIDLVQRSG
                     M"
     gene            complement(1238..>1500)
                     /locus_tag="PKN79_05925"
     CDS             complement(1238..>1500)
                     /locus_tag="PKN79_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNZ95333.1"
                     /translation="DHGNADDMYEHTKTGEVARKEDGSPKAKTSHSLNPVPFILVDPL
                     YKGEYEPTLKEGLGISSVAATVMNLLDFDPPEFYDSSIITMK"
BASE COUNT          305 a          410 c          387 g          398 t
ORIGIN      
        1 cattccggat cgttgaacca gatcgatgca gtagcgctgg cgggtatgac cgaacgccca
       61 aagaacgtga acgatttcat cgtagatatc tttggcggtc cactgcaaag aggcgtacgg
      121 ctcacaaagc ctgatgagaa tgtccgtatc ccctccggcc agcaccttga tgctttctcc
      181 ttcttctatc cgctcccgaa gaaagtgatg gatcgtcatg atctgcccca tttcgatgcc
      241 tagcagccga gtcagatatt gatcgtattt ggcaagggag ttcgtatagg tgagaagggc
      301 gacgttgctc ttttccgatt tctttccgat cgccttgaga agaacgaggg ttttccccgt
      361 tcccgggcca ccggtgatca aaaaatcgct gctcagatcg attcggcgaa gcgcctcctc
      421 ctgctgagcg gataatcgca ggataaggtg ttcataatcc cggcgggtcc gggtgtagga
      481 ggtcatcgtc cgcatcacgt gcagatactc ctcaagatcg cgggtctgct tttccaaaga
      541 gcggcgctgt acttgcagta catgccgttc atcccggagt tctttgatct ttttttcgtt
      601 tcgatccttg tctttcaaaa ggttccgaat ctgatgttcc aatgaatgga tccgatcatc
      661 aacctgctct cgttcctcct cccgttcctt cagttcggaa agctgatcgg agagcgatcc
      721 cttctcggca agtatttttt ggagctccgt ttggaggatt tgattgcttg ttatcagttc
      781 gctccggctc tccgagcggg tccaggtatc atccaactcc cgtttgatct tatagagcgt
      841 ctgcagagct ccttcatcga tatcaataaa gtaacagaac gtgtaaagat gagccgccgc
      901 catcaacgcg tggtcgacgc cgagagcatt gaactcatga cggacctcat tcaccgcctt
      961 gtgggcggct ttgagattcg ccaggaattg ccggcttttt tcgcccacct cttcgaccag
     1021 tcggtcgctg agcgcattga agctcggctc ctcttcatag ggaaggtcgt accgccggcg
     1081 cagctcccct tcgatgaacg aatggaccgc cagggcgaag attcccggat cttctccttg
     1141 aaggcggctc aggcgcttca atcgatcgtc aatacccatc cgatcctcct gcttccatct
     1201 tagcaggttt caatcggagg atgtatcaaa agatctttta cttcatcgtg atgatcgagc
     1261 tgtcgtagaa ttcgggtgga tcgaagtcaa ggagattcat caccgtggcg gcaacgctgc
     1321 tgatgcccaa tccttccttc aatgtcggtt catattcacc cttgtagagc ggatcgacca
     1381 gaatgaaggg aaccgggttg agggagtggc tcgtcttcgc tttcggcgaa ccatcctcct
     1441 tgcgggcgac ctcgccggtt ttcgtgtgct cgtacatgtc atcggcgttc ccatggtcgg
//
LOCUS       DAOPUZ010000354         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_96570_length_1494_cov_3.772759, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000354 DAOPUZ010000000
VERSION     DAOPUZ010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96570_length_1494_cov_3.772759"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PKN79_05930"
     CDS             complement(<1..664)
                     /locus_tag="PKN79_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HNZ95334.1"
                     /translation="MILSNKSLSNKKQWKEKGYRLPAYDRAAMIEETLKHPTWVHFGG
                     GNIFRGFLARLQQELLNRGLVKSGIIVGGSYDYQVIEEVFTKQDNLILAVTLKSDGSV
                     DKEVIASVAAAKPVEPAFPEDWAFFKRAFANPSLQMVSFTITEKAYAIRSNDGMLFES
                     IAKDIEEGAKAPKSFLAKLTSLLYHRYQQKGGNLTLVSMDNCSANGDRIAEAVKTIAK
                     GWA"
     gene            complement(714..>1494)
                     /locus_tag="PKN79_05935"
     CDS             complement(714..>1494)
                     /locus_tag="PKN79_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-mannonate epimerase"
                     /protein_id="HNZ95335.1"
                     /translation="GTGGADGINKSHYLGAVYGMERIMGRTDSPVRAVLDYAEKHFAQ
                     ELPLVYALTVVGRNDHGEMKPMGLFVGDDRSCYEEAAALSLEVNFTMVPEPLEKVVVY
                     LDPEEFRTTWLGNKSIYRTRMAIADEGELIVLAPAVAGFGEDEEIDRLIRKYGYKGTP
                     KVLSDVEKHKDLKDNLSAAAHLIHGSSEGRFTIRYCPGKLTREEIEEVGYEWGDLQQM
                     LQRYPIESLKDGWNTDAQGERFYYISNPAIGLWAHSGRFND"
BASE COUNT          279 a          431 c          416 g          368 t
ORIGIN      
        1 ccgcccagcc cttggcgatc gtcttgaccg cctcggcgat ccggtcgccg ttcgccgaac
       61 agttgtccat gctcaccagg gtcagattac cgcccttttg ttgatagcga tgatagagga
      121 ggcttgtcag ctttgccaaa aatgattttg gcgcctttgc tccttcctcg atgtccttgg
      181 cgatcgactc gaagagcatg ccgtcgtttg agcggatggc atacgctttc tccgtgatgg
      241 tgaagctcac catctgaagc gagggattgg cgaatgctcg tttgaagaac gcccaatctt
      301 cgggaaaggc cggttcgacc ggctttgcgg cggcgacgga ggcaatgacc tccttgtcga
      361 ccgatccgtc gcttttgagg gtaaccgcca ggatcagatt atcctgtttg gtgaagactt
      421 cctcgatgac ctgataatca tagcttccac cgacgatgat gccgcttttt accaatcccc
      481 gattcagcag ctcctgctga agacgagcga gaaagcctcg gaagatgttt ccccctccga
      541 agtgaaccca ggtaggatgc ttgagggtct cttcaatcat cgccgcccgg tcgtaggcgg
      601 ggaggcggta gcctttctct ttccattgtt ttttgttgct taggctttta ttgctcagaa
      661 tcattggtaa tcctcctttt ggatcttcat agtgctaaaa cgaaacccga cacctagtca
      721 ttgaagcgtc cgctgtgcgc ccacagcccg atggcggggt tcgatatgta gtagaagcgc
      781 tcaccttggg cgtcggtatt ccaaccatcc ttcaggctct cgatcggata gcgctgaagc
      841 atctgttgca ggtcgcccca ctcatagccg acctcttcga tctcttccct ggtgagcttg
      901 ccggggcagt aacggatcgt gaaccgtccc tcggacgatc cgtgaatcag gtgagcggcc
      961 gcagaaaggt tgtctttgag gtccttatgc ttctcgacat cggaaaggac cttcggcgtt
     1021 cccttgtagc cgtacttgcg gatcagccgg tcgatctctt catcttcgcc gaagcctgcg
     1081 acggcggggg cgaggacgat gagttcacct tcatcggcga ttgccattcg ggtgcggtag
     1141 atcgacttgt ttccaagcca cgtggtgcgg aactcctcgg gatcgaggta gacgaccact
     1201 ttttcaagag gctcgggcac catcgtgaag ttcacttcca gggagagcgc tgccgcctcc
     1261 tcgtagcagg atcggtcgtc gccgacaaaa aggcccatcg gcttcatttc tccatggtcg
     1321 ttccgtccca cgacggtaag cgcgtagaca agcgggagct cttgggcgaa gtgtttttca
     1381 gcgtaatcga gaaccgcccg gaccggggaa tcggttcgtc ccatgatccg ttccatcccg
     1441 tagaccgccc ccagatagtg gctcttgttg atcccgtccg ccccgccggt gccg
//
LOCUS       DAOPUZ010000355         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_96581_length_1494_cov_3.013204, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000355 DAOPUZ010000000
VERSION     DAOPUZ010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96581_length_1494_cov_3.013204"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PKN79_05940"
     CDS             complement(<1..671)
                     /locus_tag="PKN79_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ95336.1"
                     /translation="MKYKPLGRSGIMVSELCFGTMGFVSRTDRSSSERLYRKAREAGI
                     TFFDTANIYSGGEVEEYLGSFIRDERSEVVIATKGFGAMGSGPNDKGSNAKSLRLALE
                     GSLKRLKTDYVDIYFLHQYDPLVAEEELLRTLERFVTSGKVLSLGVSNYSAYQTMKLN
                     AAAARLGLPRIDVLQPMYSLAKRICEIEILPMAKGEGLGVISYSPLGAGLLTGRYTHE
                     DKPATG"
     gene            complement(675..>1494)
                     /locus_tag="PKN79_05945"
     CDS             complement(675..>1494)
                     /locus_tag="PKN79_05945"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNZ95337.1"
                     /translation="GELAEVYSFEKEVPHCGIGSYVSDLPILFALSFLELERVDYDHL
                     RSTYRRYIKEEPDFLEPLLEQALSNPIEKGERVFHLGRSVTSGILSALRSDSKMIAGK
                     EAALTSANAIVREAADLYGQTLISLILREAETVDELFLLLLRSRTLNDELKSLVRGAR
                     NSEEVINPQAVNTLHLRPTLLAVFSALQHATTFEDGMIALAKGGGSARLRCALYGGLA
                     GVLFDHLPESWIAELHVSSALDAMIKKQTLYRRETLTFEKIVDSLSNKLCDYEE"
BASE COUNT          294 a          424 c          458 g          318 t
ORIGIN      
        1 ccggttgcag gcttatcctc atgggtgtat cgaccggtca gaagccccgc tccgaggggg
       61 ctgtaggaga tgacgcccag accttcgcct ttggccatcg gaaggatctc gatctcgcag
      121 atccgctttg cgagcgagta catcggctgc aggacgtcga tccggggcaa accaagccga
      181 gccgctgccg cgttgagctt catcgtctga taggcgctgt agttggagac gccgaggctg
      241 aggaccttcc cgcttgtcac gaaccgttcc agggtgcgca gcagctcctc ctcggcaacg
      301 agggggtcgt attgatggag gaagtagatg tcgacatagt cggtcttcag gcgcttcagc
      361 gatccttcca gggcaaggcg caggctcttg gcgttggagc ccttgtcatt ggggccgctt
      421 cccatcgccc caaagccttt cgtcgcgatg acgacctccg agcgttcatc ccggatgaag
      481 gagccgaggt actcctcgac ttcaccgccg gaatagatgt tcgccgtgtc gaagaaggtg
      541 attcccgctt cccgggcttt cctgtagagc cgctcggagc ttgagcgatc cgtccggctg
      601 acgaagccca tcgtgccgaa gcacaattcg cttaccatga tcccgcttct tcccaaaggt
      661 ttgtacttca tctgttactc ctcatagtca cacagcttgt tgcttaagga atcgacaatt
      721 ttctcgaagg tgagcgtctc ccgcctgtag agcgtctgct tcttgatcat cgcgtccaga
      781 gccgaagaga cgtgcagttc ggcgatccag gattcgggaa ggtgatcgaa gagtacgccg
      841 gcaagtcctc cgtacaaggc gcagcgaagg cgggccgatc cgccgccctt ggcaagggcg
      901 atcatcccgt cctcgaaggt ggtggcgtgc tgcagggccg agaataccgc aagaagggtc
      961 ggacgaaggt gcagagtgtt gaccgcctgc gggttgatga cctcctccga gttccgcgct
     1021 ccccggacaa gcgatttcag ctcgtcgttc aaggtccggc ttctcagcaa aaggagaaag
     1081 agctcgtcga ccgtttccgc ttcccggagg atgagagaga tgagcgtttg accatagaga
     1141 tctgccgctt cccggacgat cgcattggcc gaggtgaggg cggcttcttt tcccgcgatc
     1201 atcttggagt cggaacgaag agcggagaga atgccgcttg ttacggatcg gcccagatga
     1261 aagacccgct ctcctttctc gatcggattc gagagcgcct gttcaagcag cggctcgagg
     1321 aaatcgggtt cttccttgat gtagcgcctg taggtcgagc ggagatggtc gtaatcgacg
     1381 cgttccagct ccagaaagga aagggcgaag aggatcggaa ggtcggagac gtagctgccg
     1441 atcccgcaat gcgggacttc cttttcaaag ctgtagactt ccgcgagttc tcca
//
LOCUS       DAOPUZ010000356         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_97005_length_1490_cov_3.535889, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000356 DAOPUZ010000000
VERSION     DAOPUZ010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97005_length_1490_cov_3.535889"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /gene="thiI"
                     /locus_tag="PKN79_05950"
     CDS             <1..1096
                     /gene="thiI"
                     /locus_tag="PKN79_05950"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HNZ95338.1"
                     /translation="TKQKGRLFLQIGDDCPREVVDTALSTTFGIVGYSRSFSAPKELE
                     AIHKAAREAIGEGPFAEGKGSFKVISRRADKSFPLTSYQLDVELAKTVHEVHPDLTVD
                     VKNPDLILYCEIRDKAYLYTTQKKGLGGLPVGSAGAGMLLLSGGLDSPVAGFLAAKRG
                     VKMDCLYFHAYPYTSDEALEKVKTLASLIAPYLQGTRLWVAHFTEAQLQIKRTAPENE
                     TTLMFRAAMMTLANRHSERTGSQCIITGEALGQVASQTLEGLSFSDSFSEQLVLRPLI
                     GMDKQEIMDLARKIGTYETSILPYEDCCVLFSPKHPLTRPDKVVATEHYEAMGIEPFI
                     EEALSNLEVFHFDERGEIIEKNHPTPDPLF"
     gene            1110..>1490
                     /locus_tag="PKN79_05955"
     CDS             1110..>1490
                     /locus_tag="PKN79_05955"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNZ95339.1"
                     /translation="MKIVRRSNQEELLELFKREIETIRQQKPEGLIHIALAGGRSMSA
                     AVRAILAQDSNTQDRMRFYLVDERLEGDKNKDTLLNEGLARPIAIPELGKPLSKEPFD
                     LVFLGIGEDGHFASLFPGTYTEDGT"
BASE COUNT          324 a          444 c          449 g          273 t
ORIGIN      
        1 caccaagcag aagggccgcc tctttcttca gatcggcgat gactgtcccc gggaggtggt
       61 cgacaccgcc ctgtcgacga cgttcggcat cgtcggctac agccgctcat tcagcgcccc
      121 caaggagctg gaggcgatcc ataaagcggc cagggaggcg atcggtgaag gtccgtttgc
      181 cgaaggcaaa ggctccttca aggtgatcag ccggcgggcg gacaagagct ttcctttaac
      241 gagctaccag ctggatgtcg agctggcgaa aacggtccat gaggtccatc ctgacctcac
      301 cgtcgatgtg aaaaaccccg atctgattct gtactgcgag atccgggaca aggcatacct
      361 gtatacgacc cagaagaaag gcctcggcgg gcttccggtc ggtagcgccg gggcgggaat
      421 gctccttctc agcggggggc tggacagtcc ggtggcggga tttttggccg ccaagcgggg
      481 ggtgaagatg gactgcctct acttccacgc ctacccgtac acgagcgacg aggcgcttga
      541 aaaggtgaag accctcgcct ccctcatcgc cccctatctc cagggaacgc gcctgtgggt
      601 ggcgcacttc acggaggccc agctccagat caagcggacc gctccggaga acgagacgac
      661 gctgatgttc cgggcggcga tgatgacgct ggccaacagg cactccgaac ggaccggcag
      721 ccaatgcatc atcaccggcg aagcactcgg ccaggtggcg agccagaccc tcgaggggct
      781 ttccttcagc gactccttca gcgagcagct ggtcctccgc ccgctcatcg ggatggacaa
      841 gcaggagatc atggacctgg cgaggaagat cggcacctac gagacctcga tccttcccta
      901 tgaggattgc tgcgtcctct tcagccccaa gcaccccctc acccgtccgg acaaggtggt
      961 cgcgacggag cactacgagg cgatggggat cgagccgttc atcgaggagg cgctctcaaa
     1021 cctggaggtg ttccacttcg atgagcgggg agagatcatc gagaagaacc accctacccc
     1081 cgatccgctt ttttgagtat ctttatccta tgaagattgt aagacgttcc aaccaagagg
     1141 aacttcttga gctgttcaag agggaaatcg agacgatcag gcagcaaaaa cctgagggtc
     1201 tcatccacat cgctttggcc ggaggccgaa gcatgagtgc ggccgtccgg gcgattctgg
     1261 cccaagattc aaacacgcag gatcggatgc gcttctacct tgtggatgaa cgcctggagg
     1321 gtgataagaa caaagacact cttctcaatg aaggacttgc ccgtccgatc gcgattcccg
     1381 agctcgggaa accgctttcg aaggagccct tcgatctggt gttccttgga atcggggaag
     1441 acgggcactt cgcttcgctg tttcccggca cctatacgga agacggaacc
//
LOCUS       DAOPUZ010000357         1486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_97417_length_1486_cov_3.079665, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000357 DAOPUZ010000000
VERSION     DAOPUZ010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97417_length_1486_cov_3.079665"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PKN79_05960"
     CDS             <1..587
                     /locus_tag="PKN79_05960"
                     /inference="COORDINATES: protein motif:HMM:NF020122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Beta-galactosidase C-terminal domain"
                     /protein_id="HNZ95340.1"
                     /translation="KTVGDGKIAYEYNINYFSAVADPDLDESLRIECFLTAQAVMLEL
                     AGVPGIYIHSLLGSENWKEGPKRDGHNRAINREKLLVDEVVRNLSNEESRRGAIHQRF
                     TRMIRARKKCRCFHPEAAQEVVESPKEIFALLRSTEGCEVLCLNNMSPHDVKTPVSVA
                     SAHDLITGRIVTADRGMVVLKPYETLWLQVMEDL"
     gene            813..>1486
                     /locus_tag="PKN79_05965"
     CDS             813..>1486
                     /locus_tag="PKN79_05965"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNZ95341.1"
                     /translation="MKSPYLVVPFGDAAGIGVEIILKALGRVKEPIVVVGDRKLIAKT
                     AQDVGLPFPFTNIVTSTNQLRTAQIEGHTPILYHLDLIDLSTFAYGEIQAQCGRGAFE
                     SVRTAVDLIEEGLVLAVVTPPLHKEALKAAGIEEIGYTEILASLSDSPKPTTMFDTHK
                     LKIFFHSRHLSLIDACKAVTKERLLQTIVTSEEITGKYPAFFDTALPLAVAGLNPHAG
                     EGGLLGR"
BASE COUNT          347 a          399 c          415 g          325 t
ORIGIN      
        1 ataagaccgt tggcgacggg aagatcgcgt atgagtacaa catcaactat ttttccgccg
       61 tcgccgatcc cgatcttgat gaatcactcc ggatcgagtg cttcctcacc gctcaggcgg
      121 tcatgcttga actggccgga gtccccggaa tctacatcca ctcccttctc gggtctgaaa
      181 actggaaaga agggccaaag cgggatggtc acaacagggc gatcaatcgg gagaaacttc
      241 tcgtcgatga agtcgtccgt aacctctcga atgaagagag tcggcggggg gcgatccatc
      301 aacgcttcac gcggatgatc cgggctcgca agaagtgccg ttgctttcat cccgaagcgg
      361 cccaagaggt cgtcgaaagt ccgaaggaga tattcgccct gcttcgctcc accgaaggat
      421 gcgaggtatt gtgcctgaac aatatgagtc cccatgatgt gaaaactccc gtgtccgttg
      481 cttcggctca cgacctgatt acagggagga tcgtaaccgc ggatcggggg atggtcgttc
      541 tgaagcccta cgagacgctg tggttgcagg taatggaaga tctttaaacg aggatcgtct
      601 tgagacgtct tgcaccgaat agtatacaac attcggtgca agcttgtgac agaaacgggg
      661 aaggggattc gctaagcaag taaggtatat acggtatcct tcctacttta tttatatgta
      721 gagcggcacg ggaagaagca aacatctcca catcccttca ttccaaacaa tggactctgg
      781 acagctctga agcgccttgt tatcatcggg ctatgaagag tccctacctc gtcgttccct
      841 ttggggacgc tgccggcatc ggtgttgaga tcattcttaa agcgcttggg cgcgtgaagg
      901 aaccgatcgt ggtggtcggt gaccggaagc tgatcgccaa aacggcccaa gatgttggtc
      961 ttccttttcc ctttaccaat atcgtaacct caaccaatca attgcgtacc gcccaaatag
     1021 agggtcatac tccgatactc taccacctcg atctcatcga tttatccacg ttcgcctatg
     1081 gcgagatcca ggcgcagtgc gggaggggag cgttcgaaag cgttcggacg gcggtcgacc
     1141 tgatcgaaga aggattggtc ttggcggtgg tgactccgcc ccttcacaag gaagccctca
     1201 aagccgccgg aatcgaggag atcggataca cggagattct ggcttcgctg agcgacagtc
     1261 ccaagccgac gacgatgttc gatacccaca agctgaagat cttcttccac agccgccacc
     1321 tttccctgat cgatgcgtgc aaggcggtga cgaaagagcg gctgcttcag acgatcgtca
     1381 cgagcgagga gatcaccggt aaataccccg ctttctttga taccgcgctt cctttggcgg
     1441 tggcgggact caatccccac gcgggggaag gcggcctgct gggacg
//
LOCUS       DAOPUZ010000358         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_98306_length_1478_cov_2.316936, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000358 DAOPUZ010000000
VERSION     DAOPUZ010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98306_length_1478_cov_2.316936"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PKN79_05970"
     CDS             <1..620
                     /locus_tag="PKN79_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNZ95342.1"
                     /translation="INLWPGQDGYDYPLQSNFVRERRWLLENITKVAKATPDIRFSLE
                     YKPKEPRNFSFMARASDTLLLAKETGLDNVGVTIDTGHAFVAGENVAEAVVILQEYGK
                     KLFHMHFNDNYGSWDDDMIVGSVHFPWYVELLFWLKETGYEGWLSMDQYPYREDGQGA
                     LRESVEFLQMIESRLTPAVMEEIRELVTKGDAVASQRWLRHTFFK"
     gene            complement(795..>1478)
                     /locus_tag="PKN79_05975"
     CDS             complement(795..>1478)
                     /locus_tag="PKN79_05975"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNZ95343.1"
                     /translation="TIIQTDISRVDNIRRDPVKVRDLMLSLARNTATMAAIKTIRDDM
                     ARGDADNALSVPTIYGYLNALERMFVVEDLPAWSPSLRSKTTIRTSPKRHFVDPSIAT
                     AVMRLTPGRLLKDFLFFGFLFESLCVRDLRIYAEGNDGQVFHYRDANGLEADAVVVLN
                     DGRWGAVEVKLGTKEIEEAAIHLRKLKERINTEKMMEPSFLLILTAGTYAYRRKDGVL
                     VVPLACLKD"
BASE COUNT          358 a          397 c          376 g          347 t
ORIGIN      
        1 ttatcaacct gtggccgggg caggatggat acgactatcc gctgcagtcg aatttcgtcc
       61 gggaacgaag gtggctcctt gagaacatca ccaaggtggc gaaggctaca ccggacattc
      121 ggttttccct cgaatacaag ccgaaggaac cgaggaattt cagcttcatg gcccgggcgt
      181 ccgacaccct cctgcttgca aaggagaccg gactggacaa cgtgggggtg acgatcgata
      241 cgggacacgc attcgtggcg ggagaaaacg tcgccgaagc ggtcgtgatt cttcaagaat
      301 atgggaagaa gctctttcat atgcacttca acgacaacta tggatcatgg gacgacgaca
      361 tgatcgtcgg ttcggttcac ttcccctggt acgtcgagct gctcttctgg ttgaaggaaa
      421 ccggctacga gggctggttg tcgatggacc agtatcccta ccgggaagac ggacaaggcg
      481 cccttcggga aagcgtggag ttcctgcaga tgatcgaaag ccgcctcacc ccggctgtca
      541 tggaggagat ccgcgaactt gtcaccaaag gggacgcagt cgcttcccag cgctggctcc
      601 gccacacgtt cttcaaataa gagacccgat gtctccccgc ggactgcggg gaggcatcct
      661 cattgttttc atcacgatac catgcgcgat gccagcgtac acgaaccggg gtgtatactt
      721 tcgaaacttc aattgttttt gttttttgaa agcgtactcc atcggttctc agaggctctt
      781 ttcatgatca catatcaatc cttcaaacat gcaagcggaa caaccagtac tccgtctttc
      841 cgacgatagg catacgtgcc tgcagtcagt atcaacaaaa aggaaggctc catcatcttc
      901 tcggtattga tccgttcctt aagcttacgc aaatggatag ccgcctcttc tatttctttt
      961 gtaccgagtt tcacctcaac ggccccccat ctgccatcat taagaaccac cacggcatca
     1021 gcttccaggc cgttcgcatc ccgatagtga aatacctgtc catcgtttcc ttccgcatat
     1081 attcgaagat cccgaacaca caaagactcg aagaggaacc caaaaaacag gaagtccttc
     1141 agcaacctgc cgggagtcaa acgcatcacg gcagtcgcga tcgagggatc gacaaaatga
     1201 cgtttcggtg atgttctgat ggttgtttta gaacgcaatg aagggctcca tgccggtaga
     1261 tcctctacga cgaacatccg ttcaagcgca ttgagatatc catagattgt tggaacggag
     1321 agggcattgt cggcatctcc tcgagccata tcatcccgga tcgtcttgat ggccgccatg
     1381 gtggcggtgt ttctggcgag ggagagcatc agatcccgaa ctttcaccgg atctcttctg
     1441 atgttgtcaa ctcttgaaat atcagtctgt atgattgt
//
LOCUS       DAOPUZ010000359         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_99007_length_1471_cov_3.841808, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000359 DAOPUZ010000000
VERSION     DAOPUZ010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99007_length_1471_cov_3.841808"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1471
                     /locus_tag="PKN79_05980"
     CDS             <1..>1471
                     /locus_tag="PKN79_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ95344.1"
                     /translation="MGGYETVSWTKEGRYAINPTAVKSWKATENADGSKTYVFNIANN
                     LVYNDGTKITAKDYVFSILYAASPQVVEIGARYGVGPEYVGYTAYHDGSAKEFKGIRL
                     LGDYSFSIQIAAEELPYYYDLSLVSVGPLPLHVIAPGVTVTDTPNGAKLSDNFTTDLL
                     RETVLTPNTGYRYRPLVTSGPYQFESYDPADSTASVILNPKFLGAYDGAKGNIYRIII
                     KLTNTATQMDELKTGAIDMLQGISGGAQITRGLDLHDQGIVNYASYPRYGYGKIAFAC
                     DFGPTQFPAVRRAIAYSLDRNEFARQYTGGYGIVVNGYYGASMWEYRENKDRLDKELN
                     SYAYNLQKAEDELVKDGWTLNKDGKAFVKGVDQIRYKKVDGELMPLSIEWANTANNPV
                     SDLIAAMLVPEVKKIGMQINGTTVDFAVLLDHLYKDGIEKPVYGMFNLGTGFATTQAI
                     WYYYSTDPEYFGTYNTNFIADKELESIALDMKRTDPQDQK"
BASE COUNT          358 a          473 c          374 g          266 t
ORIGIN      
        1 gatgggtgga tatgagacgg tgtcctggac gaaggaaggc cgctacgcga tcaaccccac
       61 cgccgtcaag tcctggaagg cgactgaaaa cgccgacggc tcgaagacgt acgtgttcaa
      121 catcgccaac aatctcgtgt acaacgacgg aacgaaaatc accgcaaaag attatgtctt
      181 cagcattctc tatgcggctt ctccccaagt tgttgaaatc ggcgcccgct acggagtcgg
      241 tcccgagtac gtaggatata ccgcatacca cgacggatct gcaaaagagt tcaaaggaat
      301 ccgcctcctt ggcgattact ccttctcgat ccagatcgcg gcggaagagc ttccctacta
      361 ctatgacctc tccctggtct ccgtcggccc gctcccgctg cacgtcatcg cccccggagt
      421 caccgtgacc gacaccccga acggtgccaa gctttccgac aacttcacca ccgacctgct
      481 gcgcgagacc gtgcttacgc cgaacaccgg ctaccgctat cgcccgcttg tcaccagcgg
      541 tccttaccag ttcgagagct acgatcccgc cgactccacc gcatcggtca ttctcaaccc
      601 caaattcctg ggtgcatatg acggtgccaa gggcaatatc taccggatca tcatcaagct
      661 gaccaacacc gcgacccaga tggacgagct gaagaccggc gcgatcgaca tgctgcaggg
      721 aatcagcggc ggagcgcaga tcacccgcgg tctcgacctc cacgatcagg gaatcgtcaa
      781 ctacgcttcc tatccccgct acggctatgg aaagatcgcc ttcgcctgtg acttcggacc
      841 gacccagttc ccggcagtcc gccgagcgat cgcctacagc ctcgatcgaa acgagttcgc
      901 ccgccagtac accggtggat atggaatcgt cgtcaacgga tactatggcg cctcgatgtg
      961 ggaataccgc gagaacaagg atcgcctgga caaagagctg aacagctatg cctacaacct
     1021 ccagaaggct gaggacgagc tggtcaaaga cggctggacc ttgaacaagg acggcaaggc
     1081 attcgtcaag ggcgtcgatc agatccgcta caagaaagtt gacggcgagc tcatgccgct
     1141 gtccatcgag tgggcgaaca ccgcgaacaa cccggtctcc gacctgatcg cggcgatgct
     1201 cgttcctgaa gtcaagaaga tcggcatgca gatcaacgga accaccgtcg acttcgccgt
     1261 gcttctcgat cacctctaca aggacggcat cgaaaagccc gtatacggca tgttcaacct
     1321 cggaaccggt tttgcgacca cccaggcaat ctggtactac tactccaccg accccgaata
     1381 cttcggaacc tacaacacca acttcatcgc cgacaaggag cttgagtcga tcgctcttga
     1441 catgaagagg accgatcctc aggatcagaa g
//
LOCUS       DAOPUZ010000360         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_99324_length_1468_cov_3.657466, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000360 DAOPUZ010000000
VERSION     DAOPUZ010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99324_length_1468_cov_3.657466"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..455)
                     /gene="rplP"
                     /locus_tag="PKN79_05985"
     CDS             complement(39..455)
                     /gene="rplP"
                     /locus_tag="PKN79_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNZ95345.1"
                     /translation="MLSPKRIKYRKKQRGSIDGIATRGNTIAFGEYGLLALEPKWITN
                     RQIEAARVAMTRHIKRGGKVWIRIFPDMPYTKKPAETRMGNGKGNPEGWVAVVKTGSV
                     MFEMGGVSEELAREALALAGSKLPIKTKFVSRREVQ"
     gene            complement(459..1169)
                     /gene="rpsC"
                     /locus_tag="PKN79_05990"
     CDS             complement(459..1169)
                     /gene="rpsC"
                     /locus_tag="PKN79_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNZ95346.1"
                     /translation="MGQKVNPIGLRLGINKTWKSKWYVDPREYADTLHEDLKLRKALL
                     TSPEASGAEISDLEIIRKPQRITMVITTSRPGVIIGSKGANVEKLGERLQKLTDKKIQ
                     IKIKEVKKPEADAQLIAMNVARQLVARGSFRRAMKIAVSKAMQSGAQGVKIRLSGRIG
                     GAEIARSEWMKEGRIPLHTLRSDIDYGFATADTSFGVVGVKVWVFNGEIYDRPVKDEA
                     GDLVRRSSRNEGLEARSL"
     gene            complement(1170..>1468)
                     /gene="rplV"
                     /locus_tag="PKN79_05995"
     CDS             complement(1170..>1468)
                     /gene="rplV"
                     /locus_tag="PKN79_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNZ95347.1"
                     /translation="PVADLVRHESYVNAVSILEAMPQKGAGLILKVLKSAAANATYLN
                     KKIDEEDLVITDLQVNEGPRLKRVWPRSHGRRDILLKRMSHITVSVDEKASGRK"
BASE COUNT          270 a          452 c          372 g          374 t
ORIGIN      
        1 cagctcctca agggtcatct ctttatagga atgtttcatt actgcacctc ccggcgggag
       61 acgaacttcg tcttgatcgg aagcttgctt cctgcaagcg caagcgcttc acgagcgagc
      121 tcttcggaaa ctccgcccat ctcgaacatc accgacccgg tcttcaccac ggcgacccat
      181 ccttccgggt ttcctttacc attacccatg cgggtttcag cgggcttctt ggtgtacggc
      241 atatcgggga aaatccgaat ccataccttt ccaccgcgtt tgatatgacg ggtcatcgcg
      301 acacgagccg cctcgatctg gcggttggtg atccatttcg gctcaagagc caagaggcca
      361 tactcgccaa atgcgattgt attgccgcgg gtggcgatgc cgtcaatgga gccacgctgc
      421 ttctttctgt atttaattct cttaggactc agcattgctt acaaactcct tgcctcaaga
      481 ccttcattcc tgctgctcct gcgaacaaga tcaccggctt cgtctttgac cgggcgatcg
      541 tagatttctc cgttgaacac ccataccttg acgccgacga caccaaagct ggtatcggca
      601 gttgcaaatc cgtagtcgat atcactgcga agggtgtgaa gaggaatccg tccttccttc
      661 atccattcgc tgcgagcgat ctcagcgccg ccgattcggc cggaaaggcg aatcttgacc
      721 ccttgggctc cactttgcat cgccttggaa acagcgatct tcatcgcccg gcggaacgat
      781 ccgcgagcga cgagctggcg cgcaacgttc attgcgatca gctgggcatc cgcttcaggc
      841 ttcttcactt ccttgatctt gatctgaatc ttcttgtcgg tgagcttctg gaggcgctcg
      901 ccgagctttt cgacgttcgc tcccttgctt ccgatgatga cgcccggccg actggtggtg
      961 atcaccatcg tgatccgctg gggctttcga atgatttcaa gatcgctgat ctccgctccg
     1021 gaggcttcgg gactcgtcaa gagcgccttt cggagcttca aatcttcgtg caacgtatcc
     1081 gcatactcgc gggggtccac ataccacttg gatttccagg tcttgttgat cccaagtctc
     1141 agaccgattg ggtttacttt ctggcccatt tacttcctcc cgctcgcttt ttcgtcgacg
     1201 ctgacggtga tatgcgacat tcgcttcaac aggatgtccc gccttccgtg gctgcgcggc
     1261 caaacgcgct tcagccgagg cccttcattg acctgcaggt cggtgatcac cagatcctcc
     1321 tcgtcgatct tcttgttgag gtacgtcgca ttcgcggcag cgctcttgag aaccttgagg
     1381 atcaggccgg cgcccttctg cggcatcgcc tccagaattg aaacggcgtt cacataggac
     1441 tcgtgccgta ccagatccgc gacggggc
//
LOCUS       DAOPUZ010000361         1466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_99572_length_1466_cov_2.894401, whole genome shotgun sequence.
ACCESSION   DAOPUZ010000361 DAOPUZ010000000
VERSION     DAOPUZ010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99572_length_1466_cov_2.894401"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..313)
                     /locus_tag="PKN79_06000"
     CDS             complement(110..313)
                     /locus_tag="PKN79_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95348.1"
                     /translation="MSIPLDSLIDYEDNIYRITCVAIKEANILSNPGLGGKEIEENNG
                     KIVTEVLSRALAGEILFEEPDDL"
     gene            complement(395..919)
                     /locus_tag="PKN79_06005"
     CDS             complement(395..919)
                     /locus_tag="PKN79_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ95349.1"
                     /translation="MRIAISGKSGCGNTTVSSLVSKELGYPLINFTFRNLAEERHMEF
                     WDLAALAEKDDSIDFELDRRQVEMALAQKDCILASRLAIWMLKEADLKIYLDATVEER
                     ARRIHKREGGTLEEKIAETTARDAKDTARYKRLYDIDNTDATVADILIDTTRKSAAEV
                     AHIIVHEVRSREKG"
     gene            complement(930..>1466)
                     /locus_tag="PKN79_06010"
     CDS             complement(930..>1466)
                     /locus_tag="PKN79_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HNZ95350.1"
                     /translation="EGVLVSISERDGELYREDLFAALDEALEQFTAAKEREGAATALH
                     LTELGKEIGASLAVIEAGADRLEEHIKQNLLKRFDELLGDKQFDENRILQEVAVMLVR
                     YSAAEEISRLGIHLKEYHSLLQTKEPVGKRLDFLCQEMNREINTIGSKSQLVELNLEV
                     VKMKNSLENIREQVRNIE"
BASE COUNT          289 a          435 c          373 g          369 t
ORIGIN      
        1 gaatggaatc ggcgttgatg acgctgtagc gtgacgagtc gaaccgactg aaatcgcggt
       61 ctgtcctacc gcagtcggtc caaagagaat gataatcggt ttgatagtct cagaggtcgt
      121 cgggctcttc gaagaggatc tccccggcaa gggctcggct gaggacttca gtgacaattt
      181 tgccattatt ctcctcgatc tctttgcccc caagtcctgg gttgctgaga atatttgctt
      241 ccttgatcgc tacgcaggta atccggtaga tattgtcttc atagtcaatc aggctatcaa
      301 ggggaatgct caccatggta ctccttcatc acagttcggc gctttacgca ccggcgtaca
      361 tcctaaaggg tttggaaaaa aaagtacagt ccccctagcc cttctctcgg ctccgaacct
      421 catgaacaat gatatgcgct acttcagccg cactttttct tgtcgtgtcg atgaggatat
      481 ccgctaccgt cgcatcggtg ttgtcgatat cgtagagtcg tttataccga gcggtatcct
      541 tggcgtctcg ggcggttgtt tcggcgatct tttcctcgag ggtgcctccc tcgcgcttat
      601 ggattcgccg cgcccgctcc tcgaccgtcg catcaagata gatcttgaga tccgcctctt
      661 tgagcatcca gatcgccaac cggctggcga ggatgcaatc cttttgggcg agggccattt
      721 caacttgccg tcggtcgagc tcaaagtcga tcgaatcatc cttctccgcc aaggcggcaa
      781 ggtcccagaa ctccatgtgc cgctcttcgg cgaggtttcg gaaggtgaag ttgatcagag
      841 gatacccaag ctccttgctg accaatgaag acaccgtggt attcccacat ccgctttttc
      901 cgctgattgc gattcgcaca cccaatctcc tactcgatat tacgcacttg ttcccgaata
      961 ttctcgaggc tgtttttcat cttcaccacc tcgaggttca gctcgacgag ctggcttttt
     1021 gatccgatcg tattgatctc ccgattcatc tcctggcaaa ggaagtcgag gcgcttgccg
     1081 accggctcct ttgtctgaag cagggaatgg tactccttca ggtgaatccc gaggcggctg
     1141 atctcctcgg cggcggagta gcggaccagc atcaccgcga cctcctgaag aatccggttc
     1201 tcatcgaact gcttatcacc cagaagctcg tcaaagcgct tcagcaggtt ctgcttgata
     1261 tgctcttcaa gccggtcggc accggcctct atgaccgcca agctcgcgcc gatctccttt
     1321 ccaagctcgg tgagatggag ggcggtcgcc gccccttccc gctccttggc agcggtgaac
     1381 tgctccagcg cctcatcgag ggcggcaaag agatcctccc ggtagagctc gccgtcccgc
     1441 tcggaaatgc ttacaagcac cccctc
//
LOCUS       DAOPUZ010000362         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_100297_length_1459_cov_2.895299, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000362 DAOPUZ010000000
VERSION     DAOPUZ010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100297_length_1459_cov_2.895299"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PKN79_06015"
     CDS             complement(<1..198)
                     /locus_tag="PKN79_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95351.1"
                     /translation="MLEFYPPTLADKNLFPKHSRYLAYNYYYSYVHLWSRQIGITIAK
                     NDTALYMHLRDDDCFLLPITDD"
     gene            391..>1459
                     /locus_tag="PKN79_06020"
     CDS             391..>1459
                     /locus_tag="PKN79_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family protein"
                     /protein_id="HNZ95352.1"
                     /translation="MDIYQDLLTRLEEVNQPLTEFFLDATYSEESFLDTLKERTEETL
                     QTIYPEGWAYLHGEKNFYRLSEPVLAHVRLYDYLVFDKAVFKDGTNEVTSRPVTLLRS
                     FLQKKSPTIHPDVAEEMVHFFALLSKKEPRAIPTRGQVQEWMERHPSGLDDEVIAWRK
                     KNKERIIDLLIRKIDERGSKEKRYTFKPGHSEKEKRWIVDEWWKEDRFHLYFALRSTK
                     ELDTFLGNTLDEETKRIMEAAEAKGIPIFVTPYFLSLIDTRPKEEQEHPFADEPIRSY
                     LFYSQDLVDEFGEITAWEKEDAVEIGKPNAAGWVLPSHNIHRRYPNVAIFIPDTKGRA
                     CGGLCAYCQRMYDFQAGRFNFE"
BASE COUNT          389 a          363 c          407 g          300 t
ORIGIN      
        1 gtcgtcggtg atgggaagaa gaaaacaatc gtcgtcccga aggtgcatat agagggcggt
       61 atcgttcttt gcgatcgtga tcccgatctg ccggctccag aggtgaacgt aggagtagta
      121 atagttgtac gccaagtagc ggctgtgctt cggaaaaaga ttcttatcgg caagggtagg
      181 gggatagaac tctagcatac atcctcgaat atatcagtcg atacgcatat attgatacga
      241 ccttttcact gcttcgtcaa cccgactcca gaggggaaac gaaagaatca tccagcggtt
      301 tttataaaat aaagagaaca taactatata ttatataata aaataggctg tcgggttgac
      361 agaattatca tcgaatacta cccttttaag atggatatct atcaagatct tttaacgaga
      421 ctcgaggaag tgaaccaacc gctgaccgaa ttctttttgg atgcaacata cagcgaagag
      481 tcatttctcg ataccctgaa agagcggacg gaagagacct tgcaaacgat ctaccccgag
      541 ggctgggcat accttcacgg ggagaagaac ttctaccggc tcagcgaacc ggttcttgcc
      601 cacgttcgct tgtacgatta cctcgtcttc gacaaggccg tcttcaaaga cggaacgaat
      661 gaagtaacat cccgaccggt cacgctcctc aggtcctttc tgcagaagaa gagtccgacg
      721 atccatcccg acgttgccga ggagatggtt catttcttcg ccctgttgag caagaaggag
      781 ccgagagcga ttcccactcg gggccaggtg caggagtgga tggagcgcca tcccagcggg
      841 ctggacgatg aggtcatcgc atggcggaag aaaaacaagg aacgaatcat cgaccttctg
      901 attcgaaaga tcgatgagcg ggggagcaaa gaaaaacgct acaccttcaa gcccgggcac
      961 tccgagaagg agaaacgatg gatcgtcgac gaatggtgga aggaagaccg cttccacctg
     1021 tacttcgcgc tgcgctcgac aaaggagctg gacacctttc tcgggaacac gttggatgaa
     1081 gagacgaaga ggatcatgga ggctgccgaa gcgaagggga ttccgatctt cgtaacccca
     1141 tacttcctct ccctaatcga cacccgtccg aaggaggagc aggaacatcc ctttgcggat
     1201 gagccgatcc ggagctacct cttctacagc caagatctgg tcgatgagtt cggagagatc
     1261 acggcgtggg agaaggagga tgccgtcgag atcgggaagc ccaatgcggc ggggtgggtt
     1321 ctgccctcgc acaacatcca ccggcgctat ccgaacgtcg cgatcttcat tcccgacacg
     1381 aagggcaggg cgtgcggggg actctgcgcc tactgccagc ggatgtacga ctttcaggcg
     1441 gggcgcttca acttcgagc
//
LOCUS       DAOPUZ010000363         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_100602_length_1456_cov_3.780871, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000363 DAOPUZ010000000
VERSION     DAOPUZ010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100602_length_1456_cov_3.780871"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PKN79_06025"
     CDS             <1..1062
                     /locus_tag="PKN79_06025"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HNZ95353.1"
                     /translation="ATGKGHLTDTTLREVFAKGEKPIEILWYREVERKHPNTLVIKAF
                     DEEDRIIASRTYYSVGGGKIVIEGEENPASVSVYPPELTKMSAILEYCNKEGLQLWEF
                     VLEVEGKEILTSMKEVWGVMSDSVERGIEEEGVLPGGLKLPRKAHSYFLKASKGAYPL
                     DDSALALSYALAVSEENASGNLVVTAPTCGSAGVLPGVLYYYAKQKNAGKIRILRALL
                     TAGLVGNLVKTNGSISGAEVGCQGEIGVACAMAGAAAAQLNGGSIFQVEYAAEMGLEH
                     HLGLTCDPMLGLVQIPCIERNALGAMRSLDHATYALLGDGRHKISFDTVVEVMLETGQ
                     ALPSLYRETSLGGLARVRS"
     gene            1096..>1456
                     /locus_tag="PKN79_06030"
     CDS             1096..>1456
                     /locus_tag="PKN79_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ95354.1"
                     /translation="MNDELLRVEGLFYSLGEDKTMLSNLSFSVQEGSFTLLCGPNGAG
                     KSTLLRILKGLYPLKAGTIVLEGKDVTKREKDRLRKIGLVFQDADSQFVGQSVEKDIR
                     FGLENLNLPLEEQNRRID"
BASE COUNT          337 a          417 c          431 g          271 t
ORIGIN      
        1 gccacgggaa aggggcacct caccgatacc acccttcggg aagtctttgc gaaaggggaa
       61 aaaccgattg agatcctgtg gtatcgggaa gtggaacgga aacacccgaa caccctcgtc
      121 atcaaggcgt tcgatgaaga agaccggatc atcgcttccc ggacctacta ctccgtcggc
      181 ggagggaaga tcgtcatcga aggggaggag aatcccgctt cggtttcggt ctacccgccc
      241 gaactgacga agatgagcgc gatccttgag tactgcaaca aggaagggct tcaactgtgg
      301 gagttcgtcc ttgaggttga aggcaaagag attctcacct ccatgaaaga ggtctggggc
      361 gtgatgagcg acagcgtcga gcgggggatc gaggaggagg gggttcttcc cggcggactg
      421 aagcttcccc gaaaagccca cagctacttc ctcaaggcga gcaaaggcgc gtatcctctg
      481 gatgattcgg ccctcgctct ctcctacgct cttgccgtca gtgaagagaa cgcctcgggc
      541 aacctggtcg tcaccgctcc tacctgcggt agcgccgggg tgcttcccgg ggtgctctac
      601 tactacgcca agcagaagaa tgccggaaaa attcggattc ttcgagccct gcttaccgcc
      661 gggctggtcg gaaacctcgt gaaaacaaac gggtcgatca gcggggctga agtcggttgc
      721 caaggcgaaa tcggcgtggc gtgcgcgatg gccggggctg ccgccgccca attgaacggc
      781 ggatcgatct ttcaggtcga gtacgctgcg gagatgggct tggaacacca cctcggactg
      841 acctgcgacc cgatgctggg attggtccag atcccctgca tcgaacgcaa cgccctcggg
      901 gcgatgcgct ccctcgacca cgcaacctat gctctgctcg gggacggccg gcataagatc
      961 agtttcgaca ccgtggtcga agtgatgctg gaaacggggc aggcccttcc ctccctctac
     1021 cgggagacga gcctcggcgg attggcccgg gtccggagtt gacaaaccaa acccataacg
     1081 gacaccctta tgcccatgaa cgacgaactg cttcgggttg aaggactctt ctattccctt
     1141 ggcgaagata aaaccatgct cagcaacctc tccttctccg tacaagaagg aagcttcacg
     1201 cttctgtgcg gtcccaacgg ggcgggcaaa agcaccctcc ttcgaatcct caaaggcctc
     1261 tatcccctca aagcgggaac gatcgttctt gaaggcaagg atgtaaccaa aagagaaaaa
     1321 gaccggcttc ggaagatcgg cctcgttttt caggacgccg acagtcaatt cgtcggccag
     1381 agcgtggaaa aagacatccg cttcgggttg gaaaacttga atcttccatt ggaggagcaa
     1441 aaccggcgga tcgatg
//
LOCUS       DAOPUZ010000364         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_100888_length_1454_cov_2.231594, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000364 DAOPUZ010000000
VERSION     DAOPUZ010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100888_length_1454_cov_2.231594"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PKN79_06035"
     CDS             <1..304
                     /locus_tag="PKN79_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HNZ95355.1"
                     /translation="SDLVRRMITEFGMSDRYRNITLPTTQSGIAGVGGSRDYSEKTQE
                     YIDNETARIVNERYEIVKANLEKNKEALLTITSELLEDEVLSGDRFEELAKSKVIG"
     gene            315..1250
                     /locus_tag="PKN79_06040"
     CDS             315..1250
                     /locus_tag="PKN79_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNZ95356.1"
                     /translation="MLYTPITDPELTRHLATLPKDGREVFVALDATVRITAISATTMI
                     NQMQTNHGLQPLETYVLGQAYLATALLTSLVEGNDRMQLSIECGGPIGGIYTEGWANG
                     SVRGYLKNNPIPLTRELTSLDLDELYGPGFLTISKLLEGKKTPFSGQTMLQWGDLAKD
                     LATHFDQSEQTPTHFILSIDFNDKAQVVGAGALFIQAMPGWKQDGLDRLANCADELPS
                     LGKHIANGYSVKSFVETHFTGVDLRHLGSTDIRFFCPCTREQYGRYIQHLKESDRADI
                     AENGPFPFEVVCANCNTTYRWSEDEMKRLLDEVKR"
     gene            1247..>1454
                     /locus_tag="PKN79_06045"
     CDS             1247..>1454
                     /locus_tag="PKN79_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95357.1"
                     /translation="MIFYATSGLHQSDILAEEARMAGAEEIVTSSSGVHFTGDLRVAY
                     RFLLTSHTATRLLLGIYEDDDIRSA"
BASE COUNT          354 a          420 c          396 g          284 t
ORIGIN      
        1 cagcgacctg gtcaggcgga tgatcaccga gttcggaatg agcgaccggt atcggaacat
       61 cacgttgccg accacccaga gcggcatcgc cggggtcggt ggaagccgtg actactcgga
      121 gaagactcag gagtatatcg acaacgagac cgctagaatc gtcaatgagc gctacgaaat
      181 cgtaaaagcc aaccttgaaa agaataagga agcgcttttg acgatcactt ccgagctctt
      241 ggaggatgaa gtcttaagtg gagaccgctt tgaagagctt gcaaaaagta aagtcattgg
      301 ctaatctcta gctcatgctc tatacaccga tcaccgaccc tgaactgacc cgacacctcg
      361 ccacccttcc caaagacgga cgcgaggtgt tcgtggcttt ggatgcaacc gtccgaatca
      421 ccgcgatcag tgcgacgacg atgatcaacc aaatgcagac caaccacggt cttcagccgc
      481 tggaaacgta cgttctcggg caggcatacc ttgcgactgc gctccttacc tcactggtgg
      541 aaggaaatga ccggatgcag ctctccatcg aatgcggagg cccgatcggc ggaatctaca
      601 ccgaaggatg ggcgaacggc tcggttcggg gatatctgaa gaacaacccg atccctctga
      661 cgcgggaact gacctctctg gatttggatg aactctacgg tccgggattc ttgacgatca
      721 gcaagctctt ggaggggaag aagaccccct tcagcggtca gacgatgctt cagtggggtg
      781 acttggcgaa ggatctggcc acccacttcg accaaagcga gcagacgccc acccacttca
      841 tcctctccat cgatttcaac gacaaagccc aagtcgtcgg cgcgggagcc ctcttcatcc
      901 aagcgatgcc cggttggaaa caggatgggt tggatcggct tgccaactgc gccgacgagc
      961 ttccttcttt ggggaagcac atcgcaaacg gctactcggt caaatcgttc gttgaaaccc
     1021 atttcaccgg ggtggatctt cgccatctcg gcagtacgga catccgattc ttctgcccct
     1081 gcaccaggga acagtacggc cggtacatcc aacacctgaa ggaaagcgac cgcgccgaca
     1141 tcgcggaaaa cggacccttt ccctttgaag tggtctgcgc gaactgcaat accacctacc
     1201 ggtggagcga agatgaaatg aaacgtctcc ttgatgaggt gaagcgatga tcttctatgc
     1261 gaccagcggc ctccatcaaa gcgatatcct ggccgaagaa gcccgcatgg cgggagcgga
     1321 agagatcgtc acgtcaagca gcggagttca tttcaccggg gatctgcgtg ttgcttatcg
     1381 cttcctgctt acgtctcaca ccgcaacgcg cctgctcctc ggcatctatg aggacgatga
     1441 tatccgcagc gccg
//
LOCUS       DAOPUZ010000365         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_101429_length_1449_cov_2.639885, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000365 DAOPUZ010000000
VERSION     DAOPUZ010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101429_length_1449_cov_2.639885"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKN79_06050"
     CDS             <1..380
                     /locus_tag="PKN79_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95358.1"
                     /translation="YLPRRRTASWYRKRYKQDVKVRGKLPALFTPVRAFGPHAPYMSL
                     KEMEEKDNGNGEEKLIFKPVASGDAGSRSKEVAEKRDETGAEKSVFTPAAFKDTPKHP
                     MTEDKVIDFLSAIEGDEAARGKK"
     gene            546..>1449
                     /gene="pyrB"
                     /locus_tag="PKN79_06055"
     CDS             546..>1449
                     /gene="pyrB"
                     /locus_tag="PKN79_06055"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNZ95359.1"
                     /translation="MGAFYGRSLCVIDDLSQEERLYLFEHARILKSAFETQDEAVLNR
                     YRIDDPDFGIYEVFLEDSTRTKESFRNAAKFHHAKVSDLNSGSSSFNKGESYADTFYT
                     LSGYSNTIFIVRSKVEGVCTYLEESLTSYARRNGLKRKPAFINAGDGKHEHPTQELLD
                     EFTFLEDNNWSTETLHLALVGDLYHGRTVHSKADGLKIFKNVTVDLIAPPELAMPETY
                     LHKMKDHGFTVRTFDSIDAYLKETAIAPKWYFTRPQLERMGDKVLKKEAELRRAITFR
                     EELIPLLPLNTKFYHPLPRHKAHPT"
BASE COUNT          372 a          390 c          398 g          289 t
ORIGIN      
        1 gctatctccc cagaaggcga accgcttcgt ggtatcgcaa acggtacaaa caggacgtca
       61 aggtccgcgg aaaactgccg gcgctcttca ctccggttcg ggcgttcggt ccgcacgctc
      121 cctacatgag cctgaaggaa atggaggaga aggacaatgg aaacggcgaa gagaaactga
      181 tcttcaagcc ggttgcctcc ggagatgcgg gtagcagatc gaaggaagtg gctgagaagc
      241 gtgatgaaac cggagcagag aaatcggtct tcaccccggc cgctttcaaa gataccccga
      301 aacaccccat gaccgaggat aaggtgattg actttctttc cgcgatcgag ggagacgaag
      361 cggcgcgcgg gaaaaaatag gcggcatcgg atggacagag tcgctcctct ttgatattgt
      421 acagaacaga aatgaggagg cttcggatga aggcacatgc actctaccgt gttcggaaat
      481 ctgaatccaa tgttgttgca cacagtcggc tttagggccg gcttttttta ttcgggagga
      541 gtacgatggg tgcgttttat ggccgatcac tgtgcgtgat cgacgatctt tcacaagaag
      601 aacggctgta cctctttgag cacgcgcgga ttctcaaatc cgcgttcgag actcaagatg
      661 aagccgtgct gaaccgatat cgaattgatg atccggactt cggaatctat gaggtgtttc
      721 tggaagattc gacgaggacg aaggaatcct ttcgcaacgc tgcgaagttc catcacgcaa
      781 aggtctcgga tctcaacagc ggttcctcct ccttcaacaa aggggaaagt tacgcggaca
      841 cgttctatac cctcagcggt tactccaata cgattttcat cgtccggagc aaagtcgagg
      901 gagtatgcac ctatctggag gagagcctca cctcctacgc cagacgaaac ggcttgaagc
      961 ggaagccggc gttcatcaac gccggtgacg gcaaacacga gcaccccacc caagagctgc
     1021 ttgatgagtt cacgttcctg gaggataaca actggtcgac ggaaaccctt cacctcgccc
     1081 tggtcggaga tctgtatcac gggcgaacgg tccactccaa agcggacggc ttgaagatct
     1141 ttaagaacgt cactgtagac ctgattgcac cgcccgagct ggcgatgcct gaaacatacc
     1201 tccacaagat gaaagaccac gggttcaccg tgcgaacctt cgactcgatc gatgcctacc
     1261 tcaaagaaac ggcaatcgcc ccaaagtggt atttcacccg tcctcagctg gaacggatgg
     1321 gtgacaaggt cctgaagaag gaagcggagc tgaggcgggc gatcaccttc cgggaagagc
     1381 tgattcccct cctgcctctg aatacgaagt tctatcatcc acttccccgc cacaaggcgc
     1441 acccgacga
//
LOCUS       DAOPUZ010000366         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_101934_length_1444_cov_3.724982, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000366 DAOPUZ010000000
VERSION     DAOPUZ010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101934_length_1444_cov_3.724982"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PKN79_06060"
     CDS             complement(<1..180)
                     /locus_tag="PKN79_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNZ95360.1"
                     /translation="MAPIETYLKQLNEPQREAVLENSRPLLMLAGAGSGKTRVITTKI
                     AYAIEALKIPPYRILA"
     gene            complement(167..>1444)
                     /gene="rimO"
                     /locus_tag="PKN79_06065"
     CDS             complement(167..>1444)
                     /gene="rimO"
                     /locus_tag="PKN79_06065"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNZ95361.1"
                     /translation="DEFSRTEDPAAADLIIVNTCGFIESARAEAIDSFFDLYGSNPEA
                     KIIFSGCMAQRYADELSAELTEASAIFGNRDLSKLPEVVGQVFSGERVVSTPEYPANS
                     FEDVYKRSRLLSFDGSAYVKISEGCNHRCSYCAIPIIRGSLRSKPMAIILEEVTSLIA
                     RGVKEINLIGQDLASWGRDLKEADAHFMALLKRIVEVEGDFVIRLLYIHPDTFPMELL
                     EFIRNHPKILPYFDIPFQHAKESILRSMGRRGTKASHLALIQKIREVLPEAVIRSTIL
                     LGYPGEDEDSFAEVMDFLIQAQLDWVGSFVYSLEEGTKAAELTNERAHRKLASKARRF
                     QQELEALQLSITQARLKTFVGRKIDVLIEEHVEGEELYIGRTYAQAPEVDGLTVVLAR
                     DLVPGTVVRCGITRVNGIDFEAVPLYGGYDGSH"
BASE COUNT          304 a          427 c          400 g          313 t
ORIGIN      
        1 ggcgagaatc ctgtaggggg ggatcttcaa cgcctcgatc gcataagcga tcttggtggt
       61 gatcacccgg gtctttccgc ttcccgcccc ggccagcatc aagagcggtc ggctgttttc
      121 aagcaccgcc tcccgttggg gttcgttcag ttgcttcaag tatgtttcaa tgggagccat
      181 catatcctcc atagagggga accgcctcga agtcgatgcc gtttacgcgg gttatcccgc
      241 agcggaccac cgttcccggt accaaatctc gggccaacac gacggtcagg ccgtccacct
      301 cgggcgcctg ggcgtaggtt cggccgatat agagctcctc gccctcgaca tgctcttcga
      361 tgagcacgtc gatctttcgg ccgacaaagg tcttcaagcg cgcttgggtg atggagagct
      421 gaagtgcttc aagctcctgt tgaaacctac gcgctttgga agcgagtttc ctgtgggctc
      481 gttcattcgt caattcggcg gctttcgtgc cctcctccag cgaataaacg aacgagccga
      541 cccaatcaag ctgcgcctga atgaggaaat ccatcacttc cgcaaacgaa tcctcatcct
      601 cgccgggata gccgagcagg atcgtcgagc ggatcaccgc ttcggggagc acctccctga
      661 tcttttgaat caacgcaagg tgtgacgcct tcgttcccct tcttcccatc gaccgaagaa
      721 tgctctcctt tgcatgctgg aacgggatgt cgaagtacgg aaggatcttc ggatggttcc
      781 gaatgaactc caacagctcc atgggaaacg tgtcggggtg gatatacagc agacggataa
      841 cgaaatcccc ctccacctcg acaatccgtt taagcagagc catgaaatgt gcatccgctt
      901 ccttcagatc ccttccccag gaggccagat cctggccgat gagattgatc tccttgactc
      961 cccgggcaat gagggaggtg acctcctcaa ggatgatcgc catcggtttg gaacgcaggg
     1021 aaccgcggat gatgggaatg gcgcagtagg agcagcgatg gttgcacccc tcgctgatct
     1081 tcacatacgc gcttccgtcg aatgagagca accgacttcg tttatagaca tcttcaaagc
     1141 tgtttgccgg atactccgga gtggagacga cccgctcgcc tgaaaagacc tgcccgacca
     1201 cctcgggcag ctttgaaaga tcgcggttgc cgaagatcgc cgatgcctcc gtcaattccg
     1261 ccgacagttc atcggcgtac cgctgagcca tgcagccgct gaagatgatc tttgcttcgg
     1321 gattgctccc gtacaggtcg aaaaaggagt cgatcgcctc agcccgggcg gattcgatga
     1381 agccgcaggt gttgacgatg atcaaatcgg cggcggccgg atcctccgtt cgggagaact
     1441 catc
//
LOCUS       DAOPUZ010000367         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_102912_length_1435_cov_3.062319, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000367 DAOPUZ010000000
VERSION     DAOPUZ010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102912_length_1435_cov_3.062319"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1435)
                     /locus_tag="PKN79_06070"
     CDS             complement(<1..>1435)
                     /locus_tag="PKN79_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95362.1"
                     /translation="LMILVTGCAFFIPLLPHISSASKQFTYQVGQLAEEDIFAPRDFI
                     HVDAEKTEERRLAAERAILPIASLNLRQTSLSYTRLDIFSKLLLSETDQRESNLYTFL
                     GSENLVDRRNLLQRLFLLKEAELAYYVATLNETASMVLSRGLMDHDSYERITSDGYDS
                     FHLEMGVQSHGDESDSLDEVLTKENLDDLLFAYLRGYIRQQSSFQPQLIIDTLVLLLE
                     NNLIYDEAKTLSLRAQARAKVELVQIAIPKNTKLVSKDTPLTANQVRMLETMNEYSFL
                     YTPTQLLARAIFTIMVTLVGCRAFILFAQGHFRSNLYLILALSVLLISVLLIGLIGWL
                     LPGTILDSHLPVLLAPMFILQITDKKRLAFITSLMISSYVALLPTSSMMTFFFSIATG
                     SLVLWTFQSAQRRLDTLSNWAYGAITTSFAALVANMLVGLDSHLYLPLVFGMIINVTV
                     SMILIEVSVPIFERLLNIPTSYRLMEIS"
BASE COUNT          382 a          332 c          404 g          317 t
ORIGIN      
        1 gagatttcca tcaatcgata ggaagtggga atattcagca atcgttcaaa gatgggaaca
       61 gatacttcga tcagaatcat cgaaacggta acgttgatga tcatcccgaa gaccagaggc
      121 aggtagagat gggaatcaag gccgacgagc atgttggcta ccagagcggc gaagctcgtg
      181 gtaatcgctc catacgccca attggaaagg gtgtcaaggc gccgctgagc ggattgaaac
      241 gtccaaagaa ccagagaacc ggtggcgata ctgaagaaga acgtcatcat cgatgaagtc
      301 ggcagcagcg ccacatagct gctgatcatc agggaggtga tgaatgccag ccgtttttta
      361 tcggtaatct gcagaatgaa catcggagcc agcaaaacgg gaagatggga gtccaagatc
      421 gttcccggca gcaaccatcc gatgagaccg atgagcagaa cggagataag gagtacgctc
      481 agtgccagta tgagatagag attgcttcga aagtggccct gggcgaagag gatgaacgcc
      541 cgacatccga caagcgtcac catgatcgtg aagatcgctc gggcaagcag ctgagtcgga
      601 gtatagagga aggagtactc gttcatcgtt tcaagcattc tcacttggtt cgcggtaagg
      661 ggggtatcct tgctcaccag cttcgtgttt ttcggaatgg cgatctgaac caattcaacc
      721 ttggcgcggg cttgagcgcg cagactcaat gtcttcgcct cgtcatagat gagattgttt
      781 tccaacagca ataccagcgt atcgatgatc agctgtggct ggaaagagct ctgttggcga
      841 atatatcccc tgaggtatgc aaagagcaga tcatcaagat tttctttggt gaggacttca
      901 tccaaggagt cgctttcatc cccatgcgat tgcacaccca tttcgaggtg gaacgaatca
      961 tatccatcac ttgtgattcg ttcatagctg tcatgatcca tcaagccgcg ggagaggacc
     1021 atgcttgccg tctcgttgag ggtggcgacg taataggcaa gctccgcttc cttcaacagg
     1081 aaaaggcgct gcaggaggtt tcttcggtca accaggtttt cgctgcccag gaatgtatac
     1141 agattgcttt cccgctgatc cgtttcactt aacagaagtt ttgagaaaat atccaagcgg
     1201 gtataggaaa gcgaagtttg tcggaggttc agcgaagcga tcggaagaat cgcccgctcg
     1261 gcggcaagtc gtcgttcttc ggttttttcc gcatcgacgt ggatgaaatc ccgcggagcg
     1321 aagatgtctt cttccgccaa ttgccccacc tggtaggtga attgcttcga agcggaagag
     1381 atatgaggaa gcagcgggat gaagaacgcg cacccggtaa ccaggatcat caaca
//
LOCUS       DAOPUZ010000368         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_103601_length_1429_cov_2.978894, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000368 DAOPUZ010000000
VERSION     DAOPUZ010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103601_length_1429_cov_2.978894"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKN79_06075"
     CDS             complement(<1..284)
                     /locus_tag="PKN79_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ95363.1"
                     /translation="MARIVLENLCKDYHVADHKTVHAVVDASLTVDDGQVVVIIGPSG
                     SGKSTLLRSINRLEEPTSGRIFIDDDEVTAKDVDIRSIREEVGMVFQSFNL"
     gene            complement(277..1095)
                     /locus_tag="PKN79_06080"
     CDS             complement(277..1095)
                     /locus_tag="PKN79_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNZ95364.1"
                     /translation="MVRKKVLSVDPHAAKKKIPTITMTDGVLIPRKDSGHLFNSWRIT
                     FISALSLLAFLVVFKPDPYRSIFLVTIKGAPVTFQATIFAIIGAIVIGTITGLGSVSK
                     KHWVNIVSGIYVETIRGIPLLVQLIFIYYAMGRFFKIEGMLAAVTALAICFGAYMGEI
                     VRAGIQAIPKGQMEAAIALGLSRTQAFRYVILPQTVKVILPAIGNEFISMLKDSSLVS
                     TIALSDILRKGREYISRTFLSLETMLIVALIYLVITLMLSRLVGIMEERLHRHG"
     gene            complement(1240..>1429)
                     /locus_tag="PKN79_06085"
     CDS             complement(1240..>1429)
                     /locus_tag="PKN79_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNZ95365.1"
                     /translation="FVLANPNYKGKLMVVGEAFTQEDIAIAVQKGNTKLLGLINKGLE
                     IAMEDGSFDALKAKWNLL"
BASE COUNT          339 a          391 c          359 g          340 t
ORIGIN      
        1 aggttgaagg attggaagac cattcccact tcttcccgaa tcgaacgaat atccacatct
       61 ttggcggtca cctcatcgtc gtcgatgaag attctcccgc tcgtcggctc ctcaagtctg
      121 ttgatcgagc gcagcaacgt agattttccg cttcccgacg ggccgatgat gacgacgacc
      181 tggccgtcat cgacggtgag cgaagcgtca accactgcat ggaccgtctt atggtcggca
      241 acgtggtaat ctttgcagag gttttccaat acgattctag ccatggcggt gcagtcgctc
      301 ctccataatt cccaccaggc gcgagagcat cagggtgatc accagataga tcaaggcgac
      361 gattagcatc gtctccaacg aaaggaacgt tcgggagatg tactcccggc ctttccgaag
      421 gatgtcgctg agggcgatcg ttgaaacgag ggaagagtcc ttcagcatcg aaatgaactc
      481 atttccgatc gcaggcagga tcaccttcac cgtttgcggc aggatcacat accggaacgc
      541 ctgcgtgcgg ctcaggccga gggcgatcgc cgcttccatc tgccctttgg gaattgcttg
      601 gataccggcc cggactattt ctcccatata cgcaccgaaa cagatcgcca gcgcggtgac
      661 ggcggcaagc atcccttcga ttttgaagaa ccggcccatc gcataataga tgaagatcag
      721 ttgaaccaga agggggatcc cccggatcgt ctcgacatag attccactga caatattcac
      781 ccaatgcttc ttgcttaccg agcccaagcc ggtaatcgtt ccaatgacga tcgctccgat
      841 gattgcgaaa atcgtcgcct ggaaggtaac gggcgctcct ttgatcgtga ccagaaagat
      901 ggaacggtag gggtcgggtt tgaaaaccac cagaaaagcc aacagactca aggcgctgat
      961 gaaggtgatc cgccacgagt tgaacagatg tccgctgtcc ttccggggaa tcagaacccc
     1021 gtcggtcatc gtgattgtgg gaatcttctt cttggccgcg tgagggtcaa ccgaaagaac
     1081 cttcttacgc accatttcga gttttacttc gtctaattcc tgattttcca tcggacattc
     1141 ttcttacata attatgcgga ttaagtcaag gttcgccgta tatttattca gacaaacaaa
     1201 aggggagccg tgctcccccc aagatcgttt catccttgat tacagcagat tccactttgc
     1261 tttcagagca tcgaaggaac cgtcctccat cgcgatttca aggcctttgt tgatcagacc
     1321 cagaagcttc gtgttgcctt tctgcacggc gatcgcgata tcttcctggg tgaacgcttc
     1381 accgacaacc atcagttttc ccttgtagtt gggattggcc aagacgaaa
//
LOCUS       DAOPUZ010000369         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_104120_length_1424_cov_3.723156, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000369 DAOPUZ010000000
VERSION     DAOPUZ010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104120_length_1424_cov_3.723156"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKN79_06090"
     CDS             complement(<1..424)
                     /locus_tag="PKN79_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNZ95366.1"
                     /translation="MRDIFHIYGTNGFEMTQILLQAIELEKLIPAQWSTIAIKPNLVV
                     AAKPEGGATTHPEIVIAIIEYLKERGFHNISIVESAWVGDSTKRAFEVNGCYAISSKY
                     DVPLVDVKDDAYVTRTVHGIPIDLSKTITETDFLISLPV"
     gene            519..>1424
                     /locus_tag="PKN79_06095"
     CDS             519..>1424
                     /locus_tag="PKN79_06095"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNZ95367.1"
                     /translation="MDLKKRAYKLLNDWKGSAYIHGLGVLDQVGEAVATLGRNALVIA
                     NTTRKSATVEKVLASLTDAGVSFGEVVPTAKPNTPKEDVLRLGKVISSRKPEVIVTIG
                     GGSAIDATKAANALATLGGAIEEYFGSDLVTKALKERNLYLLPTVAVMTAASSAAHLT
                     KYANVTDLAAGQKMLIVDNALVPTLSVFDYTSTTTMPKDLTIDGAFDSIAHTLEVYLG
                     SHSETIDLTETIADCALRLVVENAPILIDDPTNMEAREALGLASDLGGYAIMVGGTSG
                     AHLTSFSLVDIVAHGTACGIMNPYYV"
BASE COUNT          324 a          351 c          419 g          330 t
ORIGIN      
        1 ggacgggaag ggagatgagg aaatcggtct ccgtaatcgt cttgctcaga tcgatgggaa
       61 tcccatgaac cgtccgggtg acgtaggcat catcttttac gtcaacaaga ggaacatcgt
      121 acttgctgct gattgcatag cagccattga cttcgaacgc ccgtttggtg gagtctccga
      181 cccaggcgct ttcaacgatt gaaatattgt ggaatccccg ttccttgagg tattcgatga
      241 tcgcgatgac gatctcgggg tgggttgtcg ctccgccttc cggcttcgcc gcgacgacca
      301 ggttcggttt gatggcaatg gtgctccatt gggctggaat gagtttttcc agttcaatcg
      361 cctgaagaag tatttgagtc atctcgaaac cattggttcc gtagatgtga aaaatatcgc
      421 gcatgttcat tgttctgagt gtaagtcggg aacagtggtt cagtcaatct ttgactgaac
      481 aggacagtat caggtagtat atgaaagagg ggaacgatat ggatttaaaa aaacgagcgt
      541 ataaattgtt gaatgattgg aaaggttccg cctatatcca cgggcttgga gtccttgatc
      601 aggtcggtga agcggtggca accttgggca ggaacgccct tgtgatcgca aataccaccc
      661 ggaagagcgc gaccgtcgag aaggttctcg cttcgcttac ggatgcggga gtcagcttcg
      721 gtgaagtcgt gccgacggcg aagcccaaca ctccgaagga ggatgttctc cgccttggca
      781 aagtgatttc atcccgaaaa cccgaggtga tcgtcacgat cggcggcggg tcggcgatcg
      841 atgcgactaa agcggccaat gcattggcga cgttgggcgg agcgatcgaa gagtacttcg
      901 gaagcgatct ggttacaaaa gcgctcaagg agcggaatct ttacttgctt ccgacggtgg
      961 cggtgatgac cgcagcgtcc agtgcggccc acctgaccaa gtatgcgaac gtcacggatc
     1021 ttgctgcagg acaaaagatg ttgattgtcg acaacgcgtt ggtgccgacg ttgagtgtct
     1081 tcgattacac ctccaccacc acgatgccga aagacctgac gatcgacggt gcctttgact
     1141 cgatcgccca tacccttgag gtctacctcg gttctcattc cgagacgatc gatctcaccg
     1201 aaacgatcgc cgactgtgct ctccggctcg ttgtcgagaa tgcgccgatt ctgatcgatg
     1261 atccgacgaa tatggaagca cgggaggcgc tcggcctggc gagcgatctc ggcgggtatg
     1321 cgatcatggt cggaggaacg agcggagctc atctgacgag cttctccctc gtcgatatcg
     1381 tcgcccacgg aacggcgtgc ggaatcatga atccgtatta cgtg
//
LOCUS       DAOPUZ010000370         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_104134_length_1424_cov_3.249817, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000370 DAOPUZ010000000
VERSION     DAOPUZ010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104134_length_1424_cov_3.249817"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1263)
                     /locus_tag="PKN79_06100"
     CDS             complement(163..1263)
                     /locus_tag="PKN79_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ95368.1"
                     /translation="MSRKPFLLGFGAMRLPQTEEGKIDRPLATEMLRYAMEHGVTYYD
                     TAYTYHARESEPFLGEFLQNYDRASFQLATKLPQWLVESVDDARRIFEEQLKRLKVDY
                     FDYYLVHSVRKKTYVKMVDLGVITYLEKEVQAGRIKKLGFSFHSSFEDFRYILHSRAW
                     DFCQIQYNWLDIEEQAGRAGYELATEHGIPVIVMEPIKGGSLLSLPPHLKEQVQQVAK
                     EDSIAALSLRWVAEHRNVPVILSGMSTLEHVVENIATLSDPQPLTDEQHSALEEVGSH
                     LRSRLGNGCTSCSYCMPCPFGVDIPGSFNIWNTFRLFGRYEQIRKRWESMGDKGPLSC
                     TRCMTCVSLCPQEIPIPFDLARVQEELSQGAL"
BASE COUNT          320 a          377 c          394 g          333 t
ORIGIN      
        1 tgcgggactt ccttttcaaa gctgtagact tccgcgagtt ctccaagggc ttcgaccctc
       61 tcgggctcca tcccctcggt ctggctgccg aagccgtccc cgagacacca tccaagcaac
      121 gccaagcgga tcctctcttc catcatcggt caagcgcacc tcttacaggg ccccctgact
      181 gagttcttct tgaacccggg ccagatcgaa ggggatcgga atctcctgcg ggcagagcga
      241 gacgcacgtc atgcaccggg tgcaggaaag cggtcctttg tcacccattg attcccagcg
      301 ctttctgatc tgttcgtacc gaccgaacag gcggaaggtg ttccagatgt tgaaggatcc
      361 gggaatatcg acgccgaagg ggcagggcat gcagtaggaa caggaggtgc agccgttgcc
      421 gagccggctt cgcagatggg agccgacttc ctccaaggcg gaatgctgtt catcggtaag
      481 cggttgagga tcggagaggg tggcgatgtt ttcaaccaca tgctcaagcg ttgacatgcc
      541 gctgagaatc accgggacgt tcctgtgttc ggcgacccac cggagggaga gggcagcgat
      601 cgaatcttct ttcgctacct gctgaacctg ttctttcaga tgagggggca gggaaaggag
      661 cgatcctccc ttgatcggtt ccatcacgat gaccggaatc ccgtgctcgg tcgccagttc
      721 atacccggcc cggcccgcct gttcttcgat gtcgagccag ttgtattgga tctggcagaa
      781 atcccaggct cttgaatgaa ggatgtatcg gaagtcctcg aagcttgaat ggaaggaaaa
      841 gccgagcttt ttgattctgc ccgcctgcac ctctttctcc agataggtga tgactcccag
      901 gtcgaccatc ttcacatagg tcttttttct taccgagtgg acgaggtagt agtcgaagta
      961 gtcgaccttc agccgcttta gttgctcttc aaagattcgt cttgcgtcgt caacggattc
     1021 gaccagccac tggggaagct tcgtcgccag ctgaaaggag gcgcggtcat agttctggag
     1081 gaactcgccg agaaagggtt cgctctcccg ggcatggtag gtgtaggcgg tatcgtaata
     1141 ggtgactccg tgctccatcg catagcgaag catctcggta gccagcggcc gatcgatctt
     1201 tccttcctcg gtctgcggaa ggcgcatcgc gccaaatccc agaagaaacg gtttcctgct
     1261 cataaagcct ccaacatcaa gaggaagtgt agcatatttt catgaaaagt acttttgagt
     1321 ttacaaagag agaaaatatg tattcaaaag tttttagggg tataaccgga aaaactctag
     1381 aaatatatga agtatttatg gttataaccc gaataacttt ccgc
//
LOCUS       DAOPUZ010000371         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_104270_length_1423_cov_2.622807, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000371 DAOPUZ010000000
VERSION     DAOPUZ010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104270_length_1423_cov_2.622807"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PKN79_06105"
     CDS             complement(<1..387)
                     /locus_tag="PKN79_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNZ95369.1"
                     /translation="MLQPLSYVLVTPYTVAKSRTGGVLSRLLSRISLELVGAQMVALD
                     KETTEAFAQIIENRNLAACCGATRGILGSYIRQNLGPSDDGSLHRSLFLVFRGDNPTK
                     ELSTVCGTFQKDADDLEAVTGESIRDT"
     gene            complement(460..984)
                     /locus_tag="PKN79_06110"
     CDS             complement(460..984)
                     /locus_tag="PKN79_06110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNZ95370.1"
                     /translation="MKQNERLLVVVDMQNDFVTGSLGTEEAKQIIDGAVAKINAEKGS
                     VAYTLDTHFDDYLSTSEGRSLPVEHCIKGSEGHKLIPELRSPLKDAKSFEKHTFGSVE
                     LAEYIRDNPQIREVELFGLCTDICVVSNALMIKAMRPELVVKVDGRISAGTTVEKHNA
                     ALDTMESCQIVVTR"
     gene            1088..>1423
                     /locus_tag="PKN79_06115"
     CDS             1088..>1423
                     /locus_tag="PKN79_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95371.1"
                     /translation="MSDNKKPVAGVDIVDRRYVVCLLNEEGKAPRYYSGRSDTPSGQE
                     KFFSLIEGRTVVMPPDPISTTAVHRFGEAAVSIDRGYEAYRISGIKNGKRMARFVALL
                     AFHQGVKNTA"
BASE COUNT          343 a          342 c          404 g          334 t
ORIGIN      
        1 ggtgtcccga atcgattcgc cggtgacggc ttcaaggtcg tcagcgtctt tctggaaggt
       61 gccgcagacg gtgctgagct ccttcgtcgg gttgtcaccg cggaagacga gaaagaggga
      121 acggtggagc gaaccgtcat cggatgggcc caagttttgt cgaatatagg aaccgaggat
      181 gcctcgcgtt gcaccgcagc acgctgcaag atttcgattt tctataattt gggcgaacgc
      241 ttcggtagtc tctttgtcca aggcaaccat ttgggcgccg accagttcaa gtgaaattcg
      301 ggatagcaag cgggacaaaa caccaccggt tctgcttttc gcaacggtgt agggggtaac
      361 aagaacatat gataggggtt gcagcatcaa gatctcctcc aaaagggtat acactatcct
      421 actcggagga aagaaacttg tctatgaaaa ggttgataat taccgtgtta ccacgatttg
      481 acaggactcc atcgtgtcca aagcggcgtt gtgtttctca acggtggttc cggcggagat
      541 tcgtccgtct accttaacga caagttccgg tctcatcgcc ttgatcatca gcgcattgga
      601 gacgacgcag atgtcggtgc aaagaccgaa gagttcaact tccctgatct ggggattgtc
      661 tcgaatgtac tcggcaagtt caacggagcc gaaggtgtgc ttttcaaagc tcttggcatc
      721 tttgaggggg cttcgaagct cagggatcag cttatgtccc tcactgcctt tgatgcagtg
      781 ctcgacggga agggaacgac cttcactggt ggagaggtaa tcgtcaaagt gggtgtcaag
      841 ggtgtaggcg accgatcctt tttccgcgtt gatcttcgcc accgctccat cgatgatctg
      901 ttttgcttct tccgttccaa gcgagccggt aacgaaatcg ttctgcatat ccactacgac
      961 caacaggcgt tcattctgtt tcatcggttt cctcctatca gtaagaatac taagaagcga
     1021 agaaaaagaa aagaagcgcg gatttttaca actgggtttg aggtctttcc ccgggtacgc
     1081 ttgaaccatg agtgacaaca agaagccagt ggcgggagtc gatatcgtcg accgacggta
     1141 cgtagtgtgc ctcctcaacg aggaggggaa ggctccaagg tactatagcg ggcgaagcga
     1201 tacgccttcc ggacaggaaa agttcttctc tttgattgaa ggccgaaccg tggtgatgcc
     1261 acccgatccg atcagcacga ccgcagttca tcgattcggg gaagcggcgg taagcatcga
     1321 caggggatat gaagcctacc ggatctcggg aatcaagaac ggaaagcgga tggcgagatt
     1381 cgtcgctctc ttggcatttc atcaaggggt gaagaatacc gcc
//
LOCUS       DAOPUZ010000372         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_105250_length_1415_cov_2.594853, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000372 DAOPUZ010000000
VERSION     DAOPUZ010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105250_length_1415_cov_2.594853"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1039)
                     /locus_tag="PKN79_06120"
     CDS             complement(116..1039)
                     /locus_tag="PKN79_06120"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNZ95372.1"
                     /translation="MNFEVFVLGTSGMMPLPNRNLTSAMIRREGELFLFDCGEGTQVS
                     LKKLNLKWKKINSIFISHMHADHVTGLPGILMLSSQVDRDTPLTLYGPGRLKEYVDAN
                     RRILDIYINYEIIVKTVEEGVILEEEEFVVRAFELNHTKPCFGYVFEEKKRPGLFHPE
                     VAQSLGIPMGPMWGILQKGGSVTLEDGRVIQPSDVMGDLREGRKFSYVTDSLYSPRIA
                     EYVKESDLFLCEGMFTDDLVESAREKKHMTASDAARIAKEANVKQLGLLHYSPRYSDY
                     ELRYLLKDAKKVFANTVLSKDRMRFSLPLKD"
     gene            complement(1067..>1415)
                     /locus_tag="PKN79_06125"
     CDS             complement(1067..>1415)
                     /locus_tag="PKN79_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNZ95373.1"
                     /translation="EDDKRQITLLLLLGARRRMLGSSYLLSVLIVTIIALIFGVLGGI
                     LLSYAVPKVLPLIEQQSFSLFSFYLLRFSIAVPIKRILLLGALVILSSTATVFFSLHR
                     ALRKQTIGSLAFD"
BASE COUNT          340 a          375 c          384 g          316 t
ORIGIN      
        1 aatcctgcca acaggagttc cagggaagcg aattggaaat cgggttcgca tcccggggcg
       61 gtcgagccgt cgagaaggat ggaattactc aggaggcgta taaagccgct tcgttctaat
      121 ctttcaacgg aagagaaaac cgcatccggt ctttgctcag caccgtgttg gcaaagacct
      181 ttttcgcgtc cttcagcaga tagcgaagct cataatcgga gtagcgcgga ctgtagtgga
      241 gcaagccgag ctgcttcaca ttcgcctcct tggcgattcg ggcggcatcg gatgcggtca
      301 tgtgcttctt ctctcgggca ctctcgacca ggtcgtcggt gaacatccct tcgcagagaa
      361 agagatcgct ctctttgaca tattcggcga tcctcggtga atagagcgaa tcggtaacgt
      421 agctgaactt ccttccttcc cgcaaatccc ccatcacatc ggagggctga atgacccgcc
      481 cgtcttcaag ggttaccgac ccgccttttt ggaggattcc ccacatcggt cccatcggta
      541 ttccgaggct ttgagcaact tccggatgga acagtccggg gcgtttcttt tcttcaaaca
      601 catatccgaa gcacggcttg gtatggttca gctcgaacgc ccggacgaca aattcctcct
      661 cttcgaggat gaccccctcc tccaccgttt ttacgatgat ctcatagttg atgtagatgt
      721 cgagaatccg gcgattcgca tccacgtatt ctttgagcct gcccggaccg tagagagtca
      781 atggagtatc gcggtcgacc tggctggaaa gcatcaggat tcccggcagg cccgttacgt
      841 ggtcggcatg catatgggag atgaaaatcg aattgatctt cttccacttc aaattcagct
      901 ttttcagcga gacctgcgtt ccttcgccgc agtcgaagag aaacagttca ccctcgcggc
      961 gaatcatcgc gctggtgagg ttgcggttcg gcaagggcat catcccgctt gtcccgagga
     1021 caaagacttc aaaattcata cccatgagta tactgaactt tagtgtttaa tcaaaggcaa
     1081 gagagccgat cgtttgcttt cggagcgccc ggtggaggct gaaaaagacg gtagccgtgc
     1141 tggaaagaat caccaatgct cccagaagga gaattcgctt gatcggaacg gcgatggaaa
     1201 agcggagcaa atagaacgag aaaagggaaa aagactgctg ttcgatcagg ggaagaacct
     1261 tcggcaccgc ataggaaagg agtattcccc ctaagactcc gaagatcagg gcgatgatcg
     1321 tcacgatgag gacgctgagc aggtacgagg atccaagcat ccgcctccgg gcgccaagca
     1381 acaggagcag ggtgatctgc cgtttgtcat cctcc
//
LOCUS       DAOPUZ010000373         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_105324_length_1414_cov_3.968359, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000373 DAOPUZ010000000
VERSION     DAOPUZ010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105324_length_1414_cov_3.968359"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /gene="nrdD"
                     /locus_tag="PKN79_06130"
     CDS             <1..163
                     /gene="nrdD"
                     /locus_tag="PKN79_06130"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNZ95374.1"
                     /translation="HGYLVGEQKTCPHCGKATEVWTRVVGYLRPVEDFHPGKQEEHSQ
                     RSFFKAEAV"
     gene            160..843
                     /locus_tag="PKN79_06135"
     CDS             160..843
                     /locus_tag="PKN79_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNZ95375.1"
                     /translation="MIRGIEGVSFLDYPGLLSCILFLGGCNYDCFYCHNRPLLEPGEE
                     VLSLGEVESFLAERKGLLDALVVSGGEPTLHKEITPLFSIGKGLGYLTKLDTNGSRPE
                     ILKTLLEADLLDYVALDIKAPPERYREISGGSFLPVSESLNLLKRHREHEKTFSFEIR
                     TTMAPTLGREDIEQLASIVGEVPRWVLNEYRVPRTFKEEDRDRVHQKYLCKDELQGLL
                     PLHPALCLG"
     gene            complement(835..>1414)
                     /locus_tag="PKN79_06140"
     CDS             complement(835..>1414)
                     /locus_tag="PKN79_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNZ95376.1"
                     /translation="SHQHGQEIAHKDLVDFLKKEVIIPALEPSELFDKNTALYINPTG
                     RFVIGGPAGDTGLTGRKIVVDTYGGIGHHGGGAFSGKDPSKVDRSGAYLARWIATNLV
                     RSGLCTVCEVQLSYAIGVAEPISIAVETFCTGVMSEDELIRIIRRNFDLTPKGMIEAL
                     NLRAPIYRRTTNYGHFGKPDLPWERERILSLP"
BASE COUNT          280 a          392 c          435 g          307 t
ORIGIN      
        1 tcacgggtat ctcgtcggcg aacagaagac gtgcccccac tgcgggaagg cgaccgaggt
       61 gtggacccgg gtggtcgggt atctcaggcc ggttgaggac ttccatccgg gaaaacagga
      121 ggagcactcc cagcgctcct tcttcaaggc ggaggcggta tgattcgggg gatcgagggc
      181 gtctccttcc tcgattaccc cggcttgctc agttgcatcc tctttctcgg aggatgcaac
      241 tatgattgct tctactgcca taaccgcccg ctgttggagc cgggagagga agttctctca
      301 ctcggggagg tcgaatcctt tctcgccgag cgaaaaggcc tgcttgacgc cctggtggtc
      361 agcggagggg agcccacgct gcacaaggaa atcacaccat tgttttcaat cggaaaaggg
      421 ttggggtatc tgacgaagct ggataccaac ggctcaaggc ccgagatcct caaaaccctc
      481 ttggaagcgg atctcctcga ttacgtggct ctggacatca aggcgccgcc ggagcggtat
      541 cgggagatct caggcggctc cttcctgccg gtgtctgaat cgctgaacct gctgaagcga
      601 cacagggaac atgagaagac attctccttc gagatccgga ccacgatggc cccgacgttg
      661 gggagggagg atattgaaca actggcctcc attgtgggag aggtcccccg atgggttctc
      721 aacgagtacc gggtcccgag aaccttcaaa gaggaggatc gggaccgggt gcatcaaaag
      781 tatctttgta aagatgaatt acaaggactc ttgccccttc atcccgcttt gtgcctaggg
      841 tagcgacaag attcgttccc gctcccaggg tagatcaggt tttccgaagt gtccgtagtt
      901 ggtcgtccgc ctgtagatcg gagcccgtaa attgagcgct tcgatcatcc ccttgggggt
      961 gaggtcgaag tttcttcgga taatccgaat caactcatct tcgctcatca ctcccgtgca
     1021 gaaggtctcg acggcgatcg agatcggttc ggcgaccccg atcgcatagg agagctgaac
     1081 ctcgcacacc gtacagaggc cgctgcgaac cagattggtc gctatccacc gggccagata
     1141 cgctccgctg cggtccactt tggagggatc cttgccgctg aaggcgcccc cgccgtgatg
     1201 cccgattccg ccataggtgt cgacgacgat cttgcgtccg gtgagaccgg tatcgccggc
     1261 cggtcctccg atgacgaagc ggccggtggg gttgatgtac aatgcggtgt ttttgtcgaa
     1321 gagttcgctg ggttccaggg cggggatgat gacctccttt ttgaggaagt ccacgagatc
     1381 cttgtgggcg atctcctgtc cgtgctggtg gctg
//
LOCUS       DAOPUZ010000374         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_106841_length_1401_cov_3.969539, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000374 DAOPUZ010000000
VERSION     DAOPUZ010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106841_length_1401_cov_3.969539"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PKN79_06145"
     CDS             complement(<1..420)
                     /locus_tag="PKN79_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95377.1"
                     /translation="MKDTKANKLFMIALVILLALGFAWVRGEVFRGKTIRDTEAIPFA
                     KGDSLSVSAASNPIVLEIDPGATSVSLEIGRIDKKQLAVAKNGSNLSIKVSPKKRVWV
                     LSVYFGSSSSTLVVTAPPDRIKDLSLSSISGKVTVLNS"
     gene            complement(425..769)
                     /locus_tag="PKN79_06150"
     CDS             complement(425..769)
                     /locus_tag="PKN79_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNZ95378.1"
                     /translation="MATRKLYRSPNGKVFGICAGIAEWRDLNVDIVRLIVGISILTTG
                     FFPGALIYLGAALIIPMAPGGRTFEQRHGETVSDSELKSKYENLKKKVEEMESEVLNK
                     ERDWDDRFFSGK"
     gene            complement(782..1123)
                     /locus_tag="PKN79_06155"
     CDS             complement(782..1123)
                     /locus_tag="PKN79_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95379.1"
                     /translation="MTAVPILAVIFTFVIILNVLENSSKERNRKIEAALRMREMELGY
                     PPGTYSSPKWSKKFSDKDAEKAIRETYEQYASKGSTRVEMEQGIVDLEERLNNLEEIL
                     RSRKNSYNNKE"
     gene            complement(1120..>1401)
                     /locus_tag="PKN79_06160"
     CDS             complement(1120..>1401)
                     /locus_tag="PKN79_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein A"
                     /protein_id="HNZ95380.1"
                     /translation="DQIEQKRTSVKQKYQTMVDRANRAKEEEVVNTTMRRSTDDRFAE
                     MQDQIDRMQASNELNRRNASLDEQFRKLEEMEELEAELAELRKLAGGKE"
BASE COUNT          290 a          391 c          345 g          375 t
ORIGIN      
        1 cgaattgaga accgtcacct tgccgctgat cgaagagagt gaaagatcct tgatccggtc
       61 cgggggagcg gtaacaacca acgtcgacga ggaggagcca aagtagacag acagcaccca
      121 cacccgtttt ttgggtgaaa ccttgattga aaggttcgaa ccgttctttg caacggcaag
      181 ctgcttctta tcgatcctcc cgatctcaag ggagaccgag gtcgcgcccg gatcgatctc
      241 gaggacgatg gggttggaag ccgctgaaac ggaaagggaa tcgcctttgg cgaagggaat
      301 cgcctcagtg tcgcgaatgg ttttgccgcg gaagacctct ccccgcaccc aggcaaagcc
      361 aagggcgagg agaattacca aggcgatcat gaacaatttg ttggctttgg tgtctttcaa
      421 catatcattt tcctgagaag aagcgatcgt cccaatcacg ctctttgttg agtacttcgc
      481 tttccatctc ttcaaccttt ttcttcaggt tttcatactt gcttttcagc tcgctgtcgg
      541 agacggtctc cccgtgccgc tgttcaaacg tccgtccacc cggagccatc gggatgatca
      601 gggcggctcc gaggtagatc agcgctccgg ggaagaaccc ggtagtgaga atcgaaatcc
      661 ccacgatcag gcgaacgata tccacgttca aatcgcgcca ctcagcgatt cctgcacaga
      721 ttccaaagac tttaccattc ggggatcggt ataattttct tgtagccatc gttctcattc
      781 ctcattcctt attattatag gagttctttc gactccgtaa aatctcttcc agattgttga
      841 gacgctcttc aaggtcgacg atcccttgct ccatctcaac ccgggtgctg ccttttgagg
      901 catactgttc atacgtttct ctgatagctt tttcagcatc cttgtcggaa aactttttcg
      961 accacttcgg ggaggagtag gttcccgggg ggtagcccag ttccatttcc cgcattcgaa
     1021 gcgccgcttc gatcttccga ttccgttcct tggacgaatt ctccaagaca ttgagtatga
     1081 tgacgaaggt gaagatcacc gcgaggattg gtactgccgt cattccttgc ctcctgccaa
     1141 cttgcggagc tcggcaagct ctgcttcaag ctcttccatc tcttcaagct tccggaactg
     1201 ctcatcaagg gaagcgttcc ttcgattgag ttcattgctt gcctgcatcc ggtcgatctg
     1261 atcctgcatc tcggcgaacc ggtcgtcggt gcttctcctc atcgtcgtgt tgacgacttc
     1321 ctcttccttc gcccgatttg cccggtcgac catcgtctgg tacttctgtt tgacggaagt
     1381 acgcttctgc tcgatctggt c
//
LOCUS       DAOPUZ010000375         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_106967_length_1400_cov_3.191822, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000375 DAOPUZ010000000
VERSION     DAOPUZ010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106967_length_1400_cov_3.191822"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PKN79_06165"
     CDS             complement(<1..821)
                     /locus_tag="PKN79_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNZ95381.1"
                     /translation="MNGFFEILFSPDVPFIRNALFAGILSSILFGILGSVVTVKRIAG
                     LAGAISHAVLGGIGIALYLSATGIVPNLSPMVGAIVFALLSALLIGAVSLKSKQREDT
                     VIQAIWAIGMSIGVLFMAKTPGYTDPSSYLFGNILLISSRDLILLAILDVIVLFLAWR
                     FYPQIEATSFDEEFAAVRGIPTQTVFLIILSVTAVAVVLLQTFVGIVMVIAMLTLPAG
                     TAGYTARNLASMMLLSTLYSLLFSAGGLVVGWSLDLPVGAVVVVLSGLVFLGFAA"
     gene            complement(818..>1400)
                     /locus_tag="PKN79_06170"
     CDS             complement(818..>1400)
                     /locus_tag="PKN79_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ95382.1"
                     /translation="LMGGSVKTMRPEIGYIPQHATYDPSFPISVIEVVMMGLVDSTRR
                     LKKQEIRSKAIQALKQVELEDLADRPYSQLSGGQRRRVLVARALASNPRMLILDEPTA
                     NMDKESEGRLYSTLERLKGNTTILIVTHDTRFVSDLTDRVFCIDTKPEGGTGGRTVVQ
                     HALESVEGEDQKRVRHDVEIPADFCAYPRELPS"
BASE COUNT          301 a          410 c          440 g          249 t
ORIGIN      
        1 atcgcggcga agcccaaaaa gacgaggccc gagagcacga cgacgaccgc accgaccggc
       61 agatccaacg accaccccac gaccagtccc cctgcggaga agagaagcga gtacagcgtg
      121 gagagcagca tcattgatgc gaggttgcgc gccgtgtatc cggcggttcc tgcaggaagg
      181 gtcagcatcg cgatcaccat cacgattccg acgaacgtct gaagaagaac caccgcaacg
      241 gcggtaacgg acaggatgat cagaaacacc gtctgggtcg gaatcccccg aacggcggcg
      301 aactcctcgt cgaagctcgt cgcctcgatc tgcggataga agcgccaggc gaggaagagg
      361 acgatcacat ccagaatcgc cagcaggatc agatcgcggc ttgagatgag gaggatgttt
      421 ccgaagaggt agctggaagg gtcggtatag ccgggggtct tggccatgaa gagcacaccg
      481 atgctcatcc cgatcgccca gatcgcctga atcaccgtat cctcccgttg tttgctcttc
      541 agcgagaccg ctccgatcag aagtgctgaa agaagggcga agacgatcgc tccgaccatc
      601 ggcgacaggt tcgggacgat cccggtcgcc gagaggtaca aggcgattcc gatccccccg
      661 agcacggcgt ggctgatcgc tccggcaagg ccggcgatcc gtttcaccgt gacgacgcta
      721 ccgaggatgc caaacaggat cgagctgagg atgccggcga agagggcgtt gcggatgaac
      781 gggacgtcgg gtgaaaacag aatctcaaaa aaaccgttca tgatggtagc tccctcgggt
      841 aggcgcagaa gtcggccggt atctctacat cgtggcggac tcgcttctga tcctcccctt
      901 cgacgctctc caaggcgtgc tggacaaccg tccggccgcc ggtgccgccc tcgggtttgg
      961 tatcgatgca gaagacccgg tcggtcagat cggaaacgaa gcgggtgtca tgggtgacaa
     1021 tgaggatcgt cgtgtttccc ttgagacgtt ccaaggtgga gtacagccgc ccttcgctct
     1081 ccttgtccat gttggcggtc ggttcgtcga gaatcagcat tctggggttg gaggccaagg
     1141 cccgggcaac gaggacccgc cgccgctgtc ctccggaaag ctggctgtag gggcggtcgg
     1201 cgagatcttc cagttcgacc tgcttcaacg cttggatcgc tttgcttcgg atctcctgct
     1261 tcttcaacct ccgggtggaa tcgacgaggc ccatcatcac cacttcgatg acgctgatcg
     1321 gaaaggacgg atcgtaggtg gcgtgctgcg ggatgtaccc gatttcaggc cgcatcgtct
     1381 tcacgctgcc gcccatgagg
//
LOCUS       DAOPUZ010000376         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_108182_length_1390_cov_3.868165, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000376 DAOPUZ010000000
VERSION     DAOPUZ010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108182_length_1390_cov_3.868165"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="PKN79_06175"
     CDS             complement(<1..1138)
                     /locus_tag="PKN79_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNZ95383.1"
                     /translation="MTVLELSDTRRLITLADGRRIHLIGTAHVSKHSVDEVAEAIETE
                     QPDHICLELDEGRMKNKEEERSWSNMDIKKVLKEGKGFLLLANMALASFQKRMGDETE
                     TQPGAEILGAAHLAKEKEIPFSLCDREIQVTFKRAWRMSNLWNKAKLIATIISAVFSN
                     EKLSEEDLEDLKKADVMQQMMDEMAAELPAVKSVLIDERDRYLATKIYLAPGENKLAV
                     IGAGHMSGIVKTLEALDSNRLDTDVSEITVVPDSSRSSKALSWVFPVLIVSLLLYGFL
                     SFGRAEGIRMIGLWLAVNMSFTALGAILALAHPINILVSVLASPITSLHPAIGVGMVS
                     GLLQATLRKPKVRDFENLNDDATKLKGWYRNRVLHILVVFLLTSL"
BASE COUNT          280 a          377 c          394 g          339 t
ORIGIN      
        1 caagactcgt gaggaggaac accacaagga tgtggaggac tcggttccga taccacccct
       61 tcagcttcgt cgcatcatcg ttgagatttt caaaatctct gactttgggt ttgcggagcg
      121 tcgcctgcaa gaggccgctg accattccca ccccgatcgc cggatgaagg ctggtgatcg
      181 gactggccag aaccgaaacg aggatgttta tcggatgggc aagcgccaga atcgccccaa
      241 gcgcggtaaa cgacatgttc accgccaacc agagtccgat catcctgatc ccttcggccc
      301 ggccgaagga gaggaatccg tagagcagga gggagacgat caggacgggg aagacccagg
      361 aaagcgcttt ggatgatctg gaggagtcgg gaacgacggt aatctccgag acgtccgtat
      421 cgagccggtt cgagtcgagg gcttccaacg tcttgacgat tccgctcatg tgccccgcac
      481 cgatgaccgc cagcttgttc tcgccggggg caagatagat cttcgtggca aggtagcggt
      541 ctcgttcgtc gatcagaacc gacttcactg ccggcagctc ggcggccatt tcgtccatca
      601 tctgctgcat cacgtccgct ttcttcaaat cttccagatc ctcttcgcta agcttctcgt
      661 ttgagaagac cgcactgatg attgtcgcga tcagtttggc tttgttccag aggttgctca
      721 tccgccaggc ccgcttgaag gttacctgaa tctcccggtc gcagagggag aagggaattt
      781 ctttttcttt ggccaagtgc gctgccccga gaatctccgc cccgggctgc gtctccgtct
      841 cgtcccccat ccgtttttgg aaggaagcga gggccatgtt ggcaagcagg aggaatcctt
      901 tgccttcctt gaggactttc ttgatgtcca tgtttgacca ggagcgttcc tcttccttgt
      961 tcttcatccg tccttcatcc aactcaaggc agatatgatc gggctgttcg gtttcaatcg
     1021 cctcggcgac ctcatccacg ctgtgctttg agacgtgggc ggttccgatg agatggatcc
     1081 ttcttccatc ggcgagggtg atgaggcggc gggtgtcgct gagctccaag acggtcatat
     1141 gcgctaatat accgaaggta gcggtattaa acaagggaat ttgacattcc cgttcgcttt
     1201 cagctagagt cttttttata tggacaccct catctatctg gcaaagctca ccgtggcgct
     1261 cattctcggg agcctgtttt ccaaagcgat cggaaaggag cgactggttc gattcagtgc
     1321 gatcgggcag acggtgttgg tgtgggtatt gttgttcttt atgggagtca acaccggccg
     1381 gatcgagaat
//
LOCUS       DAOPUZ010000377         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_108323_length_1389_cov_2.874813, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000377 DAOPUZ010000000
VERSION     DAOPUZ010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108323_length_1389_cov_2.874813"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..>1389)
                     /gene="thrS"
                     /locus_tag="PKN79_06180"
     CDS             complement(182..>1389)
                     /gene="thrS"
                     /locus_tag="PKN79_06180"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNZ95384.1"
                     /translation="KRDHRKLGRELDLFSLHEEAGSGLVYWHPNGARIRQEIENFWRA
                     EHYANGYEMVYTPHIGKSWLWETSGHLGFYKEGMYPSMEMDNADYYVKPMNCPFHIMI
                     YNNSKRSYRDLPFRWAELGTVYRYEKAGTMHGLLRVRGFTQDDAHLFVTPDQMEEEIA
                     EVLRFSLHMLKSFGFSDIAAYLSTRPEKAVGDPKRWDDATEALRRAIEKEGLSYEVDE
                     GGGAFYGPKIDLKIKDAIGREWQLSTVQFDFNEPERFDMTFVDRDGTEKRPYMIHRAL
                     LGSLERFFGVLIEHYAGAFPPWLAPEQIKVIPVSDAYDEYAKSVVSELKKAGLRASGD
                     YSDNRMNAKIRTAQNLKIPYMVILGEREKENNEVSVRYRTGKQENSITLASFIKQVQA
                     AVAAKAQLS"
BASE COUNT          291 a          397 c          384 g          317 t
ORIGIN      
        1 gcgaaacaat cagcgagtcg cgcgtgaagg aaaacatcac gatcgtcagg atcgatgcaa
       61 gcttgaaaag gctcgggtcg atccaagagc ccaggtggta ggcgataaag aacaacggcg
      121 tgagaacgag ccgggaaacg gtaagtttat tgggaagatt cataccaacc tcgctttttc
      181 ctcaggaaag ttgcgccttc gcggcgaccg ccgcttgaac ctgcttgatg aacgaggcaa
      241 gggtgatcga gttttcctgc ttgccggtcc gatagcggac ggaaacctca ttattctcct
      301 tctctcgttc gcccaagatg accatatagg ggatcttgag gttctgagcg gtacgaattt
      361 tggcgttcat ccggttgtcg gaatagtcgc cgcttgctct caaacccgct ttcttcaatt
      421 cggaaacgac gctcttggcg tactcgtcat aggcgtcgct gaccggaatg accttgatct
      481 gctccggcgc cagccacggg gggaacgcac cggcgtagtg ttcgatcaga acgccgaaga
      541 agcgctccag cgatccgagc agggcacggt ggatcatgta tggccgcttt tcggttccat
      601 cccgatcgac gaaggtcatg tcgaagcgct ccggctcatt gaaatcgaat tgaaccgtcg
      661 acaactgcca ctcccgcccg atcgcgtcct tgatcttcaa gtcgatcttc ggtccgtaga
      721 atgctccgcc accttcatcg acttcatagc tgaggccttc tttctcaatc gcccgcctca
      781 gcgcttcggt cgcatcgtcc caacgcttgg gatcccccac cgctttttcg gggcgggtcg
      841 agagatatgc cgcgatatcg ctgaagccga agctcttgag catgtggagc gagaagcgca
      901 aaacttccgc tatttcctcc tccatctgat cgggagtcac gaagaggtgc gcatcatcct
      961 gagtgaaccc gcggacccgg agcaggccgt gcatcgtccc cgctttttcg tatcgataga
     1021 ccgtcccgag ctccgcccag cggaacggga ggtcccgata ggagcgcttg gagttgttgt
     1081 agatcatgat gtggaaggga cagttcatcg gcttgacgta gtagtcggcg ttgtccatct
     1141 ccatcgaagg gtacatccct tccttgtaga agccgagatg gccgctggtt tcccacagcc
     1201 aggacttgcc gatgtgaggg gtatagacca tctcatagcc gttggcgtag tgttccgctc
     1261 gccaaaagtt ttcaatctcc tgtcgaatcc gcgccccgtt gggatgccaa tacaccagac
     1321 ctgaacccgc ttcctcatga agactgaaaa gatccagctc gcggccgagc tttcggtggt
     1381 cccgttttt
//
LOCUS       DAOPUZ010000378         1385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_108826_length_1385_cov_2.616541, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000378 DAOPUZ010000000
VERSION     DAOPUZ010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108826_length_1385_cov_2.616541"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKN79_06185"
     CDS             complement(<1..358)
                     /locus_tag="PKN79_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl-terminal protease"
                     /protein_id="HNZ95385.1"
                     /translation="MKKFRAFHIFVFILIALTLAPVFAGGDPEPLITGLTSGRFAQDK
                     ISTDISALERLYRYIDAIYIEEVDKDKVFTDLATALVASLDDPYSFYVKPSDAKEYEE
                     GTSGIYGGIGTYLSKPN"
     gene            441..>1385
                     /gene="lgt"
                     /locus_tag="PKN79_06190"
     CDS             441..>1385
                     /gene="lgt"
                     /locus_tag="PKN79_06190"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNZ95386.1"
                     /translation="MAMYISFPSWIKPEIFSFLPIRWYGLMYILAFATAYLFIVIQAK
                     NGEIALTGEDALDLVMWCVVGLILGARLFSVLFYDGTAYYLTHPHLIFWPFRNGKFVG
                     LPGMSYHGGLFGAAAGGWLYSKKKRIPFLEIADTVVYSVPLGYTFGRLGNFINGELFG
                     RVSTKPWAMVFPDAPSFSTNYEWVRRIADELKIPYELGQYINLPRHPSQLYEALFEGV
                     LLFFFLWFVIRPKRREKPAGTGLAWYLIGYGTVRFVIEYFRSPDEHLGYIIAWGRGSD
                     TIALFQSVFNISMGQIFCLIMIVLGVAIRLITHYRSTKQ"
BASE COUNT          295 a          375 c          381 g          334 t
ORIGIN      
        1 ggttgggctt ggagaggtac gtcccgattc ctccgtatat tccgctggtt ccctcttcat
       61 actccttcgc atcggagggt ttgacgtaga aggagtaggg atcgtcaagg gaagcgacga
      121 gcgccgtagc gagatcggtg aagaccttgt ccttatcgac ctcttcgata tagatcgcat
      181 caatgtaccg atacaggcgc tcaagagcgg agatgtcagt ggaaatctta tcctgagcga
      241 atcgacccga ggtcagaccg gtaatgagcg gctcgggatc cccccctgca aaaaccgggg
      301 caagggtgag cgcaataaga ataaacacaa atatgtggaa agctctgaat tttttcatac
      361 agcaagtata ctgcaacgag tcgtcatcga ctaggtttcc cggtattgac cggggccatt
      421 tagcttacta cacttgccgc atggcaatgt acatctcctt cccaagctgg attaaaccgg
      481 aaattttctc attcctgccg attcgatggt atgggctgat gtacatcctc gcctttgcga
      541 cagcatatct tttcatcgtg atccaagcga agaacggcga gatcgccctg accggggagg
      601 atgccctcga tctggtgatg tggtgcgtcg tcggactgat tctgggagcc cggctgttct
      661 ccgtcctgtt ttatgacggc accgcgtatt atctgaccca tcctcatctc atattctggc
      721 cgttccgcaa cgggaagttc gtcggtctgc cgggaatgag ctatcatggc gggcttttcg
      781 gggctgcagc gggaggatgg ctgtactcca aaaaaaagcg gataccgttt ttggagatcg
      841 ccgacacggt cgtctacagc gttccgctgg gttacacctt cggaaggctg gggaacttca
      901 tcaacggcga actgttcggg cgggtctcga cgaaaccgtg ggcgatggtt tttcccgacg
      961 ccccatcctt ctccacaaac tacgagtggg tgcgaaggat cgccgatgag ctgaagatcc
     1021 cctatgagct cggtcagtac atcaaccttc cccggcatcc cagccagctg tatgaggcgc
     1081 tcttcgaggg agtcctcctc tttttcttcc tctggttcgt catcagaccg aagcgaaggg
     1141 agaagcccgc cggaacggga ttggcgtggt atctgatcgg ctacggaacg gtgcgcttcg
     1201 tcatcgaata tttccgctcg ccggatgaac acctcgggta catcatcgcc tgggggcgcg
     1261 gttccgatac gatcgccctt tttcaatccg tcttcaacat atcgatgggg cagatcttct
     1321 gtctgatcat gatcgtcctc ggagtggcga tccgcctcat cacccactat aggagcacca
     1381 aacag
//
LOCUS       DAOPUZ010000379         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_110396_length_1372_cov_3.792711, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000379 DAOPUZ010000000
VERSION     DAOPUZ010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110396_length_1372_cov_3.792711"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKN79_06195"
     CDS             <1..382
                     /locus_tag="PKN79_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNZ95387.1"
                     /translation="EIEGTLVNNLVKDSKTNKSRAFSADSKKGKRAVLSYRHRASSKN
                     FHLLGITPETGRHHQIRAQLAAHTIHIRGDLKYGYPRSNRDGGISLHARTLSFIHPVK
                     QERITIVAPVPDDPLWKVFEEMTT"
     gene            complement(555..>1372)
                     /locus_tag="PKN79_06200"
     CDS             complement(555..>1372)
                     /locus_tag="PKN79_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNZ95388.1"
                     /translation="PESLRYVASTFDFANGTFNEAINAVGKKIITFNGVLKYDAFPLA
                     PVVRDVLRVGSEAMGRPVEIEFAVNLNRKAPKKPEFSLLQIRPIAQGSEESDVSIDDD
                     DRRAALVHSTTVLGNGTLDSIEDLICVKLDAFDPSKMVEMANELDELNVRMVLSERDY
                     VLVVAGRLGSSDPWLGIPVSWSQISRSKVIVETGLKDLRAEPSQGTHFFQNLTSLGSF
                     YLTINPEFGQGTLDKKRLKELPIIEETKHFIHARTPLLVKVNGFTGEGVILTQ"
BASE COUNT          312 a          378 c          366 g          316 t
ORIGIN      
        1 ggagatcgag ggcacattgg tcaacaatct cgtcaaagac agcaagacga acaagagtcg
       61 tgccttttcc gctgattcaa agaaagggaa gcgggcggtc ctttcctatc gtcatcgggc
      121 atccagcaag aacttccact tgcttgggat cacaccggaa acgggaaggc accatcagat
      181 cagagcccag cttgcagccc atactattca tattcgaggg gatctgaagt acggctaccc
      241 ccgttcaaat cgcgacgggg ggatctccct ccacgcccgg accctttcct tcatccaccc
      301 cgtcaagcag gagcggatca cgatcgtcgc tccggttccc gatgatccgc tgtggaaggt
      361 gtttgaagag atgaccacgt gaaaaacccc ctgccttctt cttgcagggg ggaagtcgaa
      421 taataccaat tccaacattg tggtttttgc agaaccactc catctgaatg cattctacca
      481 tgggtttcgc atgggggcaa agactttttt atcttttttt gacacctttg ctgaacatga
      541 cgcgataatg ccgtttactg tgtcagaatc accccttcac cggtaaaccc gttcactttg
      601 acgagaagcg gcgttcgggc atggatgaaa tgcttcgtct cttcgatgat tgggagctcc
      661 ttcagccgct tcttatccaa tgttccctga ccgaactccg ggttgatcgt caggtagaag
      721 gaacccaagc tggtaaggtt ttggaagaag tgggttccct ggctcggctc ggcgcgcaga
      781 tccttcaagc cggtctcgac gatcaccttg cttcgtgata tctgggacca ggaaacggga
      841 atcccgagcc aagggtcgga ggaacccagt cgaccggcaa ccacgaggac gtaatcacgt
      901 tcggaaagga ccatgcgaac attcaactca tccagctcgt tggccatctc gaccatcttg
      961 gagggatcga aggcatcaag cttgacgcag atgagatcct cgatcgaatc gagggttccg
     1021 ttccccagca cggtggtgga gtggaccaat gcggctcgtc ggtcatcatc gtcgatcgaa
     1081 acgtcgcttt cttccgaacc ttgggcgatc ggacggattt gcaggaggct gaactccggc
     1141 ttcttcggag cttttcggtt taggttgacc gcaaactcga tctctaccgg cctgcccatc
     1201 gcctccgagc cgacacgaag cacatcccgg accaccgggg caagggggaa cgcatcatac
     1261 ttaagcaccc cgttgaaggt gattatcttc tttccgacgg cgttgattgc ttcattgaac
     1321 gttccgttgg cgaaatcgaa agtggaggcg acgtatcgaa ggctttccgg gt
//
LOCUS       DAOPUZ010000380         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_111420_length_1364_cov_2.407945, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000380 DAOPUZ010000000
VERSION     DAOPUZ010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111420_length_1364_cov_2.407945"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PKN79_06205"
     CDS             <1..952
                     /locus_tag="PKN79_06205"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNZ95389.1"
                     /translation="LFVIMRDLASKGVSMIYISHRLDEIGIITDRVTVLRDGELVGTK
                     DTDKLSKDEMISMMVGRVVYEKPKLKSTVPKGAPIALEVKNLHAGKLVQDVSFYVRKG
                     EILGFAGLMGAGRTETMRALYGADRSVGNIWINGEKVQINTPIDAVRLGIGYLSEDRK
                     RYGLAIGLSVAENSVMASYEELCPKLFIPQNELEDISKEYVDRLEVRTPSLDQLVKNL
                     SGGNQQKVVIAKWLIKNSSVLIFDEPTRGIDVGAKSEIYHLMNELAQEGKAIIMISSE
                     LNEILRMSDRVAVMCEGKLTGILDIEDASQERIMALATQH"
     gene            970..>1364
                     /locus_tag="PKN79_06210"
     CDS             970..>1364
                     /locus_tag="PKN79_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ95390.1"
                     /translation="MKSLKHGELTEKKGVNMQKLLAPLALVIVYGFFAIFGRNFFSYT
                     TLVNILDSSYYIGFIAIGVTFVIITGGIDLSCGTIMMAAAIIGGTAYKTWGWPMWLSL
                     LLILVVSTLFGTLNGLLVSRLHLPPFIAT"
BASE COUNT          342 a          341 c          368 g          313 t
ORIGIN      
        1 tctgtttgtc atcatgcgcg accttgcttc caagggagtc agcatgatct acatctccca
       61 ccgccttgat gagatcggaa tcatcaccga ccgggtgacg gtccttcgtg acggcgaact
      121 cgtcggaacg aaagacaccg ataaactttc caaagacgag atgatcagca tgatggtcgg
      181 ccgggtcgtg tacgaaaaac cgaagctcaa gtccaccgtt cccaaaggcg ctccgatcgc
      241 cctggaagtg aaaaaccttc atgccggcaa acttgtccaa gatgtgagtt tttatgtacg
      301 caagggcgag attctcggct ttgccggcct gatgggcgcc ggacgaaccg aaacgatgcg
      361 cgccttgtat ggtgctgacc gctccgttgg caacatctgg atcaatgggg aaaaagtcca
      421 gatcaataca ccgatagatg cggtgagatt gggaatcgga tacctcagcg aagaccgtaa
      481 gcgttacggg ttggcaatcg gcctcagcgt cgcggaaaac tcggtgatgg cctcctacga
      541 agagctgtgc cccaaactgt tcataccgca aaatgaactt gaagatatca gcaaagagta
      601 tgtcgatcgg cttgaagtcc ggactccgag cctcgatcag ttggttaaaa acctctcggg
      661 aggaaaccag cagaaggtgg tcatcgccaa atggctgatc aaaaacagct cggttctgat
      721 ctttgatgaa ccgacgcggg gaatcgacgt cggagcgaag agtgaaatct accatctgat
      781 gaatgagctt gctcaggaag gtaaagcgat catcatgatc agcagtgagc tgaacgaaat
      841 tttacggatg agtgaccggg ttgctgtgat gtgtgaaggc aaattaaccg gaattctgga
      901 tatcgaagat gcaagccagg aacggatcat ggctcttgca acccaacact gagagaggag
      961 tatcgtcgta tgaagtcttt gaaacatgga gagctaacgg aaaagaaggg agtgaacatg
     1021 cagaaactcc tcgccccgct tgctttggta atcgtctacg gtttttttgc catctttggt
     1081 cgtaacttct tttcctacac aacgctggtg aacatcctcg actcctcgta ctatatcggc
     1141 tttatcgcca tcggggtgac ctttgtcatc atcaccggag gaatcgacct ttcctgcggg
     1201 acgatcatga tggccgccgc aatcattggc ggaaccgcgt acaagacgtg gggctggccg
     1261 atgtggctga gccttttact gatcctcgtc gtttcgactc tcttcggaac gcttaacggt
     1321 cttctggtca gccggcttca ccttccgccg ttcatcgcga cgat
//
LOCUS       DAOPUZ010000381         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_112115_length_1359_cov_2.014571, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000381 DAOPUZ010000000
VERSION     DAOPUZ010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112115_length_1359_cov_2.014571"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PKN79_06215"
     CDS             complement(<1..1107)
                     /locus_tag="PKN79_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNZ95391.1"
                     /translation="MIDGLITVAALSPGLRVADPSFNGKEIVAGIEEAAKQGVCLLVL
                     PELCTTGSTCGDLFYSQTLLKASDDAVREIVGSTSSRELIVVFGAPVAFGSNLYNASI
                     VAYDGQILAIVPKPAGGVFASYEGEPLSVELFDAYTYFGNGIRIAPSAIPHFVLAIDD
                     FTTPSGATVVASPISEAELIGRSTKRRLLAQGFTISNACALVQANSGEGESTTDSVYL
                     ADALIVERGKILSESGGMSGKLVIGQIDLQVLVHQRRLEKRYPLYEEGCLEIPCDLVH
                     APSSLIRHIDPAPFVPHDPVRLKERCEEVLTLQALGLKKRLEHIGIKRVILGLSGGLD
                     STAALLAVIRTFDVMSLDRGGIIAVSMPGFGTTNR"
BASE COUNT          322 a          377 c          386 g          274 t
ORIGIN      
        1 ccggtttgtt gttccgaacc ccggcatcga gaccgcaatg atgcccccgc ggtcaaggct
       61 catcacatca aacgtgcgga tgacggcaag cagggctgcc gttgaatcaa gtcccccgga
      121 gagaccgaga atcacccgtt tgatgccgat atgttcaaga cgtttcttca ggccgagggc
      181 ttggagggtg agcacttctt cgcagcgctc cttaagccgc accggatcat ggggaacgaa
      241 cggcgccggg tcgatatggc gaatcaagga ggatggagca tgaaccagat cacagggaat
      301 ttcaaggcag ccctcttcgt agagggggta cctcttttca agccggcgct gatggacaag
      361 cacctgaaga tcgatctgtc cgattacgag ctttccgctc attccgccgc tctcactgag
      421 gatttttccc cgttcgacga tcaatgcatc ggcaagatag acggaatcgg tcgtcgactc
      481 cccttctccg ctgttcgcct gaacgagagc acaggcgttg gaaatcgtaa agccctgagc
      541 gagaagccgg cgtttggtgg aacgaccgat cagctccgct tcactgatcg gggaagcgac
      601 caccgtagct ccgctcggtg tcgtaaaatc atcgattgca agaacgaaat gaggaattgc
      661 cgatggggcg atgcgaatcc cgttgccgaa gtaggtgtac gcatcaaaca gctcgacgga
      721 gagcggctcg ccctcatatg aagcgaagac gccgccggcg ggtttgggta ctatcgcaag
      781 gatttgcccg tcataggcga cgatcgaagc gttatagaga tttgatccga aggcgacggg
      841 cgctccgaag acgacgatca gctcacgaga agatgtagaa ccaacgattt ctcgtaccgc
      901 atcatcgctc gctttgagga gggtttggga gtagaagaga tccccgcacg tcgacccggt
      961 ggtgcaaagt tcgggtagta cgagaaggca aacaccctgt tttgccgctt cttcaattcc
     1021 ggcgacgatc tctttgccgt tgaacgaagg atctgcaacc ctgagcccgg gcgaaagcgc
     1081 cgcaaccgta atcaatccgt caatcatcgt cgacctcctc tccacaccct cagtgtaccg
     1141 cccaagaata gtggtgggca agaggcggtt gtgtggtatt ctaatgccat ggcagtgatc
     1201 gcaatcaatg acgtttccaa gcacttcgga aaaaccaaag cccttgacgg ggtgaccctg
     1261 gcgatcgaag aaaacacgat tcacggattt ctcggtccca acggggcggg taagaccacg
     1321 atgatccgaa tcctccttgg aatcatccgc cccactcgg
//
LOCUS       DAOPUZ010000382         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_113019_length_1352_cov_3.259059, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000382 DAOPUZ010000000
VERSION     DAOPUZ010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113019_length_1352_cov_3.259059"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PKN79_06220"
     CDS             complement(<1..607)
                     /locus_tag="PKN79_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNZ95392.1"
                     /translation="METLRTCFTEMTAFFDRGESRSLAFRKTQLLSLRTALLEHEEEI
                     LEALSSDLQKSKVESWMTELGIVLEQIAYHLKRIDTWAKPQRVRTTLSTFPSSSYTIA
                     EPKGVVLIISPWNYPLQLTLVPLISAIAAGNCAIVKPSRQSRHTTLVIEKILSSIYPA
                     HYIAAVTEGDLLSLPFDHIFFTGSTRVGKLVMKSASEHLSSV"
     gene            complement(683..>1352)
                     /locus_tag="PKN79_06225"
     CDS             complement(683..>1352)
                     /locus_tag="PKN79_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNZ95393.1"
                     /translation="IENLYIDIGVSSQQEACDLGVRVGNPIVPKAELEFKNNGIICGK
                     AFDDRLGCAAVVEVLEAIRGESLSVNPVGVFSSQEEVGLRGAQVAANTLKPDVAICFE
                     GTPADDTFESCDRQQTVLGKGPMLRYIDSKMITNPRFQRFALDVAKKYDIRVQTAVRT
                     GGATNAGAIHLSGGGVPSIVIGVPVRYAHTHYSVASLDDLKGAIALGKAILKELNRKI
                     IASF"
BASE COUNT          291 a          336 c          404 g          321 t
ORIGIN      
        1 tgacggaaga caggtgttcg cttgcgctct tcatgacgag cttgccgacc cgggtcgaac
       61 cggtgaagaa gatatggtcg aacggaaggg aaaggaggtc gccctcggta acggcggcga
      121 tgtaatgggc cgggtagatg gaagaaagga tcttttcgat cacgagggtg gtatgtctgc
      181 tctgtctgga gggtttcacg atcgcgcagt tgccggcggc gatcgcgctg atcaggggta
      241 ccagcgtaag ttgaagggga tagttccagg gactgatgat gagcaccacg cccttcggtt
      301 cggcgatcgt ataggaagag gagggaaagg tggagagggt cgttcgaact cgttgcggtt
      361 tggcccacgt atcgatccgt ttcagatggt aggcgatctg ttccagaacg attccgagct
      421 cagtcatcca tgattcgact ttggacttct gcaagtccga cgataacgct tcgagaatct
      481 cttcttcgtg ttcaagcagg gcggttctca aggaaagcag ctgagttttc cgaaacgcaa
      541 gggatcgact ctctccccga tcgaaaaagg cggtcatttc ggtgaaacag gttcttagtg
      601 tttccatgag agcagtatct caccgttgaa gcgggtttgt caaaacgcca gaccgtttat
      661 ttcgacatta tccaaagaat tgttaaaaag aagcgatgat ctttcggttc agctctttga
      721 gaatcgcttt gccaagggcg atcgctcctt tcaggtcgtc gaggctcgcc accgaatagt
      781 gggtgtgggc ataccggaca ggaacaccga tgacgatcga gggaacgccc ccaccgctca
      841 ggtggatcgc tcccgcattc gtcgctcctc cggtgcgaac ggcggtttga acccggatgt
      901 cgtatttctt tgcaacatcg agggcaaagc gctggaagcg gggattggtg atcatctttg
      961 agtcgatgta gcggagcatc ggtcctttgc cgaggaccgt ctgctggcgg tcacagcttt
     1021 cgaaggtgtc gtcggcgggc gttccttcaa aacagatcgc cacatccggt ttcagcgtat
     1081 tggcggcgac ttgggctcct cggagtccga cctcttcctg gctggagaag acccccaccg
     1141 ggttgacgga cagtgattct cctcgaatcg cttccaacac ctcgacgacc gcagcacacc
     1201 caaggcggtc gtcaaacgct ttgccgcaga tgattccatt gtttttgaat tcaagttccg
     1261 ctttgggaac gatcggattg ccgactcgaa cgccaaggtc acaggcttcc tgttgggagg
     1321 acactccgat gtctatataa aggttttcga tt
//
LOCUS       DAOPUZ010000383         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_113033_length_1352_cov_3.045490, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000383 DAOPUZ010000000
VERSION     DAOPUZ010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113033_length_1352_cov_3.045490"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKN79_06230"
     CDS             <1..239
                     /locus_tag="PKN79_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNZ95394.1"
                     /translation="HCMTAISATIFLVSVRWTLLTTRILESMTELQFSQASAFSIVLI
                     AIVFLADFILKHVLKFTTAHITRSQEISYEQIGA"
     gene            220..1335
                     /locus_tag="PKN79_06235"
     CDS             220..1335
                     /locus_tag="PKN79_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ95395.1"
                     /translation="MNKSALELIGVSKLFQKDGESVLAVDAVGLTVKEGELVTFLGPS
                     GCGKTTTLRMVAGFELPTSGRITIKGKDMTNVPVNKRGIGFMFQTYALFPHMSVYNNV
                     AYGLKARGEKDRETKTKVREALRLVGLEKTEHRFPNQLSGGEQQRVALARVIVMEPSL
                     LLMDEPLSNLDAKLRIHMRTEIRKIQQDLGITCLYVTHDQSEALTMADRIVIMNKGRI
                     EQIGTPFEIYSNPQSVFVADFIGQANIIRLKIMAVGSDTISVQIPRGGVFTARALRPE
                     RLKKQEAIYKVGAEASLIIRPEQIQLGPAPAKEDQFPAIVESAMFVGSHIEYFVKLPN
                     DSVIKALVPYQTGMHIFSAGDDVELSFDVASAVFLPN"
BASE COUNT          305 a          351 c          390 g          306 t
ORIGIN      
        1 tccactgcat gaccgcgatc agcgccacca ttttcctggt atcggtccgt tggaccttgc
       61 tcacgacgag aatccttgaa agcatgaccg agctccagtt ctctcaggca tcggcgttct
      121 cgatcgtcct gatcgcgatc gtgtttctcg ctgatttcat tttgaagcat gtattgaaat
      181 tcacgactgc tcacattact cggtctcagg agatttccta tgaacaaatc ggcgcttgag
      241 ttgattggtg tttccaagct cttccaaaaa gatggcgaat ccgtccttgc ggtggatgct
      301 gtaggtctta cggtcaagga gggcgaactg gtaacgtttt tgggcccgtc ggggtgcggg
      361 aagaccacga ccctccggat ggtcgccgga ttcgagctgc ccacaagcgg tcggatcacc
      421 atcaaaggaa aggatatgac taacgtcccg gtgaacaagc ggggcatcgg gttcatgttc
      481 cagacctacg cgctgtttcc gcatatgagc gtgtataaca acgtcgcata tgggttgaaa
      541 gcgcgagggg aaaaggatcg cgagacaaaa accaaagtgc gggaagcgct ccgtcttgtc
      601 gggctggaga agaccgagca tcgatttccc aatcaactga gcgggggaga gcagcagcgc
      661 gtcgcgctgg ctcgagtgat cgttatggag ccaagcctcc tgttgatgga tgaacccctt
      721 tccaaccttg atgcgaagct gagaatccac atgcggaccg agattcgcaa gattcagcag
      781 gatctcggca tcacctgcct ctatgtcacc cacgatcagt cggaggcttt gacgatggcg
      841 gatcgaatcg tgatcatgaa caagggtagg atcgaacaga tcggaacgcc gttcgagatc
      901 tacagcaatc cgcaatcggt attcgtggcg gatttcatcg gacaggccaa catcatccgc
      961 ttgaagataa tggccgtcgg ctcggatacg atttccgtgc agattccccg gggaggggtg
     1021 ttcaccgccc gggcattgcg acccgagcgg ttgaagaagc aggaggcgat ctacaaggtt
     1081 ggtgccgagg catccttgat cattcgtccg gagcagatcc agttgggacc cgctccggcg
     1141 aaggaagatc agtttccggc catcgtcgaa tcggcgatgt tcgttggaag ccatatcgaa
     1201 tacttcgtga agctgcccaa cgattcggtg ataaaggcgt tggtccccta ccagaccggc
     1261 atgcacatct tctcggcggg ggatgacgtg gaactgagtt tcgatgtggc cagcgccgtc
     1321 ttccttccca attagactgt aacgttcagt tt
//
LOCUS       DAOPUZ010000384         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_113192_length_1351_cov_2.547068, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000384 DAOPUZ010000000
VERSION     DAOPUZ010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113192_length_1351_cov_2.547068"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PKN79_06240"
     CDS             <1..746
                     /locus_tag="PKN79_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ95396.1"
                     /translation="TVDELFDKDGFDAIFVGSGAGLPRFLDIEGENSIGVFSANEYLT
                     RSNLMKAYAKTNALTPMYRSKRVAVFGAGNVAMDAARTAKRLGAEVVSIIYRRTEAEM
                     PARREEIAHAKEEGIEFLYLLAPKRIIPGEDGRVKEVEVIECKLGPEDEDGRRRSMMI
                     EGTESLRSYDTVIVAIGNDSNPLIKATTDGIETSHKGTFIVDPSTCATSLEGVYAGGD
                     IVLGAATVILAMGQGRKAARAMNEYLATL"
     gene            complement(747..1217)
                     /locus_tag="PKN79_06245"
     CDS             complement(747..1217)
                     /locus_tag="PKN79_06245"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNZ95397.1"
                     /translation="MFFKEGGSEETPLFQKSDWVFGFMPLYCEVDIAPVLQAALQTRR
                     LALPRSNGDDTLTFVEVKSLDELKPGRFGILEPKASVEAQPTSASLIIVPALAYTPKR
                     VRLGRGKGFYDRYLERFPDPVSIGVCRTWQLIEELPVSIYDRQVDSLLCNGTWY"
BASE COUNT          318 a          362 c          411 g          260 t
ORIGIN      
        1 gcacggtgga tgaactcttt gacaaggacg gcttcgatgc gatcttcgtc ggcagcggag
       61 cgggtctccc ccgatttctg gatatcgagg gagagaactc gatcggggtg ttcagcgcca
      121 atgaatacct gactcgcagc aacctgatga aagcctacgc caaaaccaac gcgttgacac
      181 cgatgtaccg ctccaagcgg gtagcggtct tcggagcggg caacgttgcg atggatgccg
      241 cccgaaccgc caagcgtctc ggagcggagg tggtgagcat catctaccgc cggacggagg
      301 cggagatgcc cgcccggcgg gaggagatcg cccacgccaa agaggagggg atcgaattcc
      361 tctacctcct tgcccccaag cggatcattc ccggcgagga tggccgggtg aaagaggtcg
      421 aggtcatcga atgcaagctg ggaccggaag atgaggatgg gcgccggcgc tcgatgatga
      481 tcgaggggac ggagtccctc cgatcgtacg acaccgtgat cgtcgcaatc ggaaatgatt
      541 ccaaccccct catcaaagcg acgacggacg gcattgaaac cagccacaag ggaacattca
      601 tcgtggatcc ttcgacgtgc gctaccagcc tggaaggggt gtatgcggga ggggacatcg
      661 ttttgggggc ggccacggtc attctggcga tgggccaggg aagaaaagcc gcaagagcga
      721 tgaacgaata cttggcgact ctgtaatcaa taccacgttc cgttgcagag caaggaatca
      781 acctgccggt catagatgct gaccggcagt tcttcgatca gctgccaggt tcgacagacg
      841 ccgatgctca ccgggtcggg aaagcgctcc agataccggt cgtaaaaacc ctttccccgg
      901 ccgaggcgca ctcgcttggg ggtatacgcc aaagccggga cgatgatcag ggaggctgag
      961 gtgggctgag cctcaacact tgctttcggt tcaaggatcc cgaagcgccc cggcttcagt
     1021 tcatcaagac tcttcacttc cacgaaggtg agggtgtcat cgccattgga acggggaagt
     1081 gccaagcgcc ttgtctgaag agccgcttgc aaaacgggtg caatatccac ctcgcaataa
     1141 agcggcataa agccgaagac ccaatcgctt ttttgaaaaa gcggggtttc ctccgaacct
     1201 ccttccttga aaaacacaag ctttgggctg aagatcagag aaattttcac taattcagtg
     1261 gcattgccct atatcccggc ttatcagatc agcttgtcga ggaagcttgt caggggcaaa
     1321 aatttcggtg tggtcggttc gatggtgttt t
//
LOCUS       DAOPUZ010000385         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_113249_length_1351_cov_1.698302, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000385 DAOPUZ010000000
VERSION     DAOPUZ010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113249_length_1351_cov_1.698302"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKN79_06250"
     CDS             complement(<1..286)
                     /locus_tag="PKN79_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ95398.1"
                     /translation="MKKTLLVILIIGLAFGTLFAAGTKEEAKAQKEIVILVKNMGNGF
                     FDAVFKGSEEAAAELGGIKTVYMGPSQPTAEGQIEIIESLIAQRVDGIAIS"
     gene            complement(340..>1351)
                     /locus_tag="PKN79_06255"
     CDS             complement(340..>1351)
                     /locus_tag="PKN79_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNZ95399.1"
                     /translation="LVLLGGGNTSYKEGNILHVKVSGHPLGSIDEGGFVRMDRSKLEA
                     IWNQKFSNDDARREAEVLQVMMASRLEGETARPSVEALLHALLPFTYVVHLHPSLVNG
                     LTCAVEGAKYARELFPEALWIDLVKPGFILADTIRRAMMPLESVPNLIILQNHGIFVG
                     GESLEEIDELYRHVLSTLKSNLKRDVDERSVEIDSTIVDPIKEELKTLFSGEVEYFHS
                     NDTRSLLESAESFYPVSSSFTPDHIVYSGFRPLFVPKGDSITEAFEAFTKKHGQNPKV
                     VCVQDVGVFADGEKPLMLFIDTVRISVYTESFGGPLFMTDEMIEFIRTWEVEQYRAKV
                     TT"
BASE COUNT          287 a          384 c          340 g          340 t
ORIGIN      
        1 cactgatcgc gattccatcg acgcgctggg cgatcaatga ttcgatgatc tcgatctgtc
       61 cttcagcggt cggctggctg ggtcccatat atacggtctt gatcccgccc agctcggcag
      121 cggcttcctc gcttcctttg aacaccgcat caaagaatcc gttacccatg ttcttcacaa
      181 ggatgacgat ctctttctga gctttcgctt cttccttggt tccggcagcg aacagcgtac
      241 cgaaggccaa gccgatgata agaataacaa gcagtgtttt cttcatcttt gttctctcct
      301 tttcttcata ttctatgttc tcctgatatt caggctaact cacgtagtta cttttgcccg
      361 atattgctcc acttcccatg ttcgaatgaa ttcgatcatc tcatcggtca tgaacagcgg
      421 tccgccgaag ctttcggtgt acacgctgat ccgcaccgtg tcgatgaaca gcataagggg
      481 cttttctccg tcggcgaaca ccccgacatc ctgaacacag accaccttgg ggttttgtcc
      541 gtgcttcttc gtgaaggctt caaacgcttc ggtgatcgaa tctcctttag gcacgaagag
      601 cggcctgaac cccgaataga cgatgtggtc gggggtgaag gaggatgaga ccggataaaa
      661 gctctccgcg ctttcgagca gcgacctcgt gtcgttggaa tggaaatact cgacttctcc
      721 tgaaaagaga gtcttgagct cctccttgat cggatcgacg atcgtcgaat cgatttccac
      781 cgatctttca tccacatccc gtttgaggtt cgatttcaac gtggaaagca catgccggta
      841 gagctcgtcg atctcctcaa ggctctcccc tcccacaaag atgccgtggt tctgaaggat
      901 gatcaggttg gggacgctct ccaagggcat catcgccctc cggatcgtgt cggcgagaat
      961 gaagccgggt ttcaccagat cgatccagag cgcctcggga aagagctctc tggcgtattt
     1021 tgctccttcg accgcacagg tcagtccgtt gacgagagag ggatgcaggt gcacgacgta
     1081 ggtaaacggc aagagcgcat ggagcagcgc ttcgacggaa ggacgggcgg tctccccttc
     1141 gaggcgcgag gccatcatca cctgcagcac ttccgcttcc cgcctggcat catcgtttga
     1201 gaacttttga ttccaaattg cttccagctt ggagcgatcc attcgaacga acccgccctc
     1261 atcaatcgat ccgaggggat ggccgctcac cttcacatgg aggatgtttc cttctttgta
     1321 tgaggtgttt ccacccccga gaagaacaag a
//
LOCUS       DAOPUZ010000386         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_113573_length_1348_cov_3.010828, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000386 DAOPUZ010000000
VERSION     DAOPUZ010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113573_length_1348_cov_3.010828"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..871
                     /gene="argB"
                     /locus_tag="PKN79_06260"
     CDS             8..871
                     /gene="argB"
                     /locus_tag="PKN79_06260"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNZ95400.1"
                     /translation="MLKAETLIEAIPYIRRFAGAITVVKYGGSAMVKPELKKAVIEDI
                     AMLKYIGLKPVVVHGGGAEISNLLARLGKESVFIDGLRVTDEETASVAEMVLSGSIGK
                     SLVDNLEQVGIKAVGISGKDGRTFLCEKKKDEKGRDLGFVGEIKKVDTTLVDSLLKSN
                     FVPVLSPVGVDTEGNTYNINADYAASAIAGALSAQKLIFLTDVEGILKDKDDPSTIIR
                     TLTHGEAVSYIEQGIIKGGMIPKVQCCLDGLEKGVESVHVLDGRLPHAILLEIFTTRG
                     IGTMVTKEKTP"
     gene            868..>1348
                     /locus_tag="PKN79_06265"
     CDS             868..>1348
                     /locus_tag="PKN79_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ95401.1"
                     /translation="MSNNHLMNTYSQWPVTFISGEGAYLKDDKGDAYLDFVGGIAVNA
                     LGYNHEGLAAVIREVLDSGVLHVSNLYYNAYVLEAAKKLAALSGMEKVFFCNSGAEAN
                     EAALKLARKWGSGKNKSTIISMLDSFHGRTYAAVTATGQKKYHQHFAPLPPGFAYAHF
                     "
BASE COUNT          320 a          350 c          414 g          264 t
ORIGIN      
        1 ccaggagatc ttgaaagcgg aaaccctcat tgaagcgatc ccgtatatcc ggcggttcgc
       61 cggcgcgatc actgtcgtga agtacggcgg ttcggcgatg gtgaagcctg aactgaagaa
      121 agcggtcatc gaagatatcg cgatgctgaa gtatatcggt ctgaaaccgg tagtcgtcca
      181 cggcggcgga gcggagatct caaatctcct tgcccgcctc ggcaaggaga gcgtcttcat
      241 cgacggactg agggtgacgg atgaggagac cgcttcggtg gccgagatgg tgctttccgg
      301 ctcgatcggg aaatccctgg tggacaactt ggagcaggtc ggcatcaagg cggtggggat
      361 cagcggcaag gacggccgga cgttcctgtg cgaaaagaaa aaggatgaga aaggccggga
      421 cctcggcttt gtcggagaga tcaaaaaggt cgacaccacc ttggtggatt ccctcttgaa
      481 gagcaacttc gtccccgtcc tttccccggt cggggtcgac accgaaggaa atacctacaa
      541 tatcaacgcc gattatgccg ccagtgcgat cgccggagcg ctgtcggccc aaaagctgat
      601 ttttttgacc gatgtggagg ggattctcaa ggacaaggac gatccttcca cgatcatccg
      661 gaccctgacc cacggcgagg cggtctccta tatcgagcag gggatcatca aaggcgggat
      721 gattcccaag gttcaatgct gtctggacgg gctggaaaaa ggggtcgaga gcgtccacgt
      781 cctcgacggg cgactgcccc atgcgattct ccttgagatc ttcaccactc ggggaatcgg
      841 aacgatggtc accaaggaga aaacaccatg agcaacaacc atctgatgaa tacctattcg
      901 caatggcctg tcacgttcat aagcggcgaa ggggcgtatc tgaaggatga caagggcgat
      961 gcgtatctcg atttcgtcgg agggatcgca gtcaacgcgt tgggatataa ccatgagggg
     1021 cttgccgccg tcatccggga agtccttgat tcgggagtgc tccatgtttc caacctctac
     1081 tacaacgcct atgtgctgga ggcggcaaag aaacttgccg ccctgagcgg gatggagaag
     1141 gtattcttct gcaacagcgg agcggaagcg aatgaagcgg cgttgaagct tgcccggaag
     1201 tgggggagcg gaaagaacaa atccacgatc atctcgatgc ttgattcctt tcatggccgg
     1261 acatatgcgg cggtgacggc gaccgggcag aaaaagtacc atcagcactt cgcgccgctt
     1321 cccccgggat tcgcctatgc acatttca
//
LOCUS       DAOPUZ010000387         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_114146_length_1344_cov_2.642358, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000387 DAOPUZ010000000
VERSION     DAOPUZ010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114146_length_1344_cov_2.642358"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="PKN79_06270"
     CDS             <1..1231
                     /locus_tag="PKN79_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNZ95402.1"
                     /translation="KVWEESTAARILHLVEESSAKKAPSERFITTFARYYTPTVVASA
                     LLLFLIPLIITGSHQPWLYRALVFLVVSCPCALVISVPLSYFAGIGKSAKSGIIVKGG
                     NYLEALAKADCAIFDKTGTLTTGSFTIASIDLFDTDYSEEQVARLAASLERHSTHPLA
                     RAFAEIPHARDVTDIQEHAGKGIVGTIGHQRIALGNNALYEVLGIPMEERGALLLSVD
                     GFLVARFNVVDAIKDEAPALMKRLRSYGVATIGMISGDSKKNAEAVGLALGLDEVHAA
                     LLPHQKQETMIEMGKRFPDYFYVGDGMNDAPSLAASKVGIAIGSGASDAAIEHADVVI
                     IHDDVREVGNLISISRKTARIVRQNIALALGVKALAMIFAAFGYAPMWVAVIADTGVT
                     ALAVLNALRLLIFNPSR"
BASE COUNT          289 a          395 c          376 g          284 t
ORIGIN      
        1 caaggtctgg gaggaatcga cggcggcccg aattctccat ctcgtggagg agtcctcggc
       61 aaagaaagcc ccgtccgagc ggttcatcac cacgttcgcc cgctattaca ccccgactgt
      121 cgtcgcttcg gccctgctgc tcttcctgat tccgctcatc attaccggca gtcatcaacc
      181 gtggctctat cgggcgttgg tctttttggt ggtcagctgc ccctgcgctc tggtcatcag
      241 cgtaccgctt tcctactttg ccggaatcgg gaaaagcgcg aagagcggaa tcatcgtcaa
      301 gggcggcaac taccttgaag cgctggccaa ggcggattgc gcgatcttcg ataaaaccgg
      361 gaccctcacc accggatcgt tcacgatcgc ctccatcgac ctctttgaca cggattattc
      421 cgaagaacag gtcgctcgct tggccgcatc gctggaacgg cactccaccc acccgcttgc
      481 ccgggcgttt gcggagattc cccatgcccg ggatgttacc gacattcagg aacatgccgg
      541 caaggggatc gtcggaacca tcggacatca acggatagcc ctcggaaaca acgccctgta
      601 tgaagtgctg ggaatcccca tggaggaacg aggagcgctt ctgttgagcg tcgacggctt
      661 ccttgtggcc cgcttcaatg ttgtcgatgc gataaaggac gaagctcccg ctctgatgaa
      721 gcgccttcga tcctatggcg ttgccacgat cggaatgatc agcggtgaca gcaaaaagaa
      781 cgccgaagcc gtgggtctcg cccttggatt ggacgaggtc catgcagcgc tgctgcccca
      841 ccaaaaacag gaaacgatga ttgaaatggg aaaacggttc cccgattatt tctatgtcgg
      901 tgacgggatg aacgacgccc catcccttgc ggcaagcaag gtgggaatag cgatcggcag
      961 cggtgccagc gacgccgcaa tcgaacacgc cgacgtggtc atcatccacg atgacgtgag
     1021 ggaagtcgga aacctgattt cgatcagcag gaagaccgcc cggatcgtca ggcagaacat
     1081 cgccctcgcc ttgggtgtga aagccctggc gatgatcttt gccgcctttg gctatgcccc
     1141 gatgtgggtt gcggtcatcg ccgatacggg ggtgacggct ctggcggtcc tcaatgcatt
     1201 gaggctgctc atcttcaatc cttcacgttg attctccctc ttttgtatgg tatctttgca
     1261 acgctttaca aggaccacgc gatgaacgaa caattaatcc gaaatatcgg gatcatggcc
     1321 cacattgatg ccggaaagac gacc
//
LOCUS       DAOPUZ010000388         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_114562_length_1341_cov_2.255832, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000388 DAOPUZ010000000
VERSION     DAOPUZ010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114562_length_1341_cov_2.255832"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..>1341
                     /locus_tag="PKN79_06275"
     CDS             3..>1341
                     /locus_tag="PKN79_06275"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNZ95403.1"
                     /translation="MFLDRYALKDVKKETLAVGDIVVAVSNPRTGQREIGTVTSIKDG
                     DEIVVTLDGGVVLKVKREEIDKPIETEPGQMLNRVAKGIAAQEKKEIRSKWEKEFNWI
                     LEDWRFVPGGRILTGAGTDQNLTYFNCYVIPSPKDSRGGIMTSLGQMTEIMSRGGGVG
                     MNLSSLRPRHAYVKGVNGRSSGSVSWGGLFSFVTGLIEQGGSRRGALMLILNVWHPDI
                     MDFINSKREMGKITNANISVGITDEFMAAVRSDKEWNTVFPDTSDPKYNTDWDGDLEA
                     WIKKGGSTVVYQTLKAKEVWNAIVESAWASAEPGVFFMDRYNQMSNSWYYSSIQSTNP
                     CGEQGLPPWGVCNLGSINLSQFVKDKTVLWDDLGRTVRSSVRFLDNVIDDTPYFFEEN
                     RKQQQGERRIGLGTMGLADMLIKMELAYGSEPSLKFIDELYKFICVEAYDESCN"
BASE COUNT          314 a          367 c          425 g          235 t
ORIGIN      
        1 agatctttct ggatcgatac gccctcaaag atgtcaagaa agaaacgctc gctgttggcg
       61 atatcgtcgt tgcggtcagc aacccccgga ccgggcaacg ggagatcgga acagtcacgt
      121 ccatcaagga cggcgacgag atcgtcgtca ccttggatgg cggggtggtc ttgaaagtaa
      181 agcgggaaga gatcgacaag ccgatcgaga ccgagccggg tcagatgctg aaccgcgtcg
      241 ctaaagggat cgccgctcag gagaagaaag agatccgaag caagtgggag aaggagttca
      301 actggatctt ggaggattgg cgcttcgttc cggggggaag gattctcacc ggggcgggaa
      361 ccgatcagaa cctgacatac ttcaactgct atgtgatccc ctcgcccaag gacagccggg
      421 gcgggatcat gaccagcctg gggcagatga ccgagatcat gagccgcggc ggcggagtgg
      481 ggatgaacct ctcctccctg cgcccccggc acgcgtacgt caaaggggtg aacgggcgct
      541 ccagcggatc ggtcagctgg ggagggctct tcagcttcgt caccggcctg atcgagcagg
      601 gcggctcccg gcggggggcg ctgatgctga ttctcaacgt ctggcatccg gacatcatgg
      661 atttcatcaa ctccaagcgg gagatgggca agatcaccaa cgccaacatc agcgtcggca
      721 tcaccgacga gttcatggcg gcggtccgct ccgacaagga gtggaacacc gtctttccgg
      781 atacctccga cccgaaatac aacaccgatt gggatggcga tctggaggcc tggatcaaga
      841 aaggcggttc cacggtcgtc taccagacgc tcaaggcgaa agaggtctgg aacgcgattg
      901 tcgagagcgc ctgggcaagc gccgaaccgg gggtgttctt catggatcgg tacaatcaga
      961 tgagcaactc ctggtactac tcttcgatcc aatccaccaa tccctgcggc gagcagggcc
     1021 tcccgccgtg gggggtgtgc aacctcggtt cgatcaatct cagccaattc gtcaaggaca
     1081 agacggtgct gtgggacgat ctcggacgga cggtccgctc aagcgtgcgc tttctggaca
     1141 acgtcatcga tgacacgccg tatttcttcg aagagaacag gaagcagcag caaggggaac
     1201 gaaggatcgg gctcgggacg atgggccttg cagacatgct gatcaagatg gagctcgcct
     1261 acggaagcga accctccttg aagttcatcg acgagctcta caagttcatc tgcgtcgaag
     1321 catacgacga gagctgcaat c
//
LOCUS       DAOPUZ010000389         1336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_115204_length_1336_cov_3.245902, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000389 DAOPUZ010000000
VERSION     DAOPUZ010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115204_length_1336_cov_3.245902"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PKN79_06280"
     CDS             complement(<1..917)
                     /locus_tag="PKN79_06280"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNZ95404.1"
                     /translation="MQEPVLEINYLSKSFAKNKVLDNVAVTVHKGRVLGLMGENGAGK
                     STLVKCLFGIYAKDEGQMFLNGKPIDFKNPKEALENGVAMVHQELNQCLDRSITDNLF
                     LGRYPLRFGVIDEARMLMEANQLFASLNMNINPRTIMRTMSVSHRQMVEIAKAVSYDA
                     KIIVLDEPTSSLTEREVKKLFSIIDDLRKKGVSFIYISHKMDEVFEICDDVEVLRDGK
                     MVLSKPVAETTMDEIVSALVGRSLDKRYPDVDNNPGEEYFHIRNLTTKFDPVLQDISF
                     SVRKGEIFGLYGLVGAGRSELLEALFGIRT"
     gene            complement(993..>1336)
                     /locus_tag="PKN79_06285"
     CDS             complement(993..>1336)
                     /locus_tag="PKN79_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding lipoprotein"
                     /protein_id="HNZ95405.1"
                     /translation="KAVMAANSGVDSTNWKQYTEGSRDAGIKQSAAPKKRVLLTIYNA
                     SDNFLSSSYVPALQYYAPLIGIDLTIVQGDGQNESSTLDKFTNLGSFDGYAINMVKTN
                     SGPDYLDKLRY"
BASE COUNT          281 a          324 c          380 g          351 t
ORIGIN      
        1 gtccggattc cgaagagcgc ttcgagcagc tcgcttcttc ctgctccgac cagcccgtag
       61 aggccgaaaa tctcgccttt tcgaacggag aaggagatat cctgcaaaac ggggtcaaac
      121 ttcgtagtca gattgcggat atggaagtac tcttccccgg ggttgttgtc gacatcgggg
      181 tagcgcttgt caagggagcg gccgacaagg gcggagacga tctcatccat ggtggtttca
      241 gcaacgggct tggaaagaac catctttccg tccctgagta cttcaacatc gtcacagatc
      301 tcgaagacct catccatctt gtgcgatatg taaataaagg agactccttt tttgcgcaaa
      361 tcatcgatga tcgagaagag cttcttcact tcacgctcgg tgagcgaaga ggtcggctcg
      421 tcaaggacga tgatcttcgc atcataggag accgctttgg cgatttcgac catctggcgg
      481 tgtgaaacgc tcattgtcct catgatggtt cgggggttga tgttcatgtt cagggatgcg
      541 aacagctggt ttgcttccat cagcatccgg gcttcgtcga tcacgccgaa gcgaaggggg
      601 tatcggccaa ggaaaaggtt gtcggttatc gagcggtcca ggcactgatt cagttcctga
      661 tgcaccattg cgaccccgtt ttcgagtgct tccttggggt tcttgaagtc gattggcttg
      721 ccgtttaaaa acatctgtcc ttcatctttg gcgtagatgc cgaagaggca tttgaccaat
      781 gtcgatttgc ccgccccgtt ttcccccatg aggccgagga ctcgaccttt gtggacggtg
      841 accgcgacat tgtcgaggac tttgtttttg gcgaaggatt ttgagaggta gttgatctct
      901 aatactggtt cttgcatggc attctcaggt agatttggaa aagcatgcgg gaacgtcccg
      961 ccccgcatgc ttcctgttca aaattggtgc ttttagtacc ggagcttatc caaatagtcc
     1021 ggaccggagt tggtcttgac catgttgatg gcatagccgt caaagctacc aaggttggtg
     1081 aacttatcca aggttgagga ttcgttctgt ccgtcgccct ggacgatcgt gagatcgatt
     1141 ccgatcagcg gtgcatagta ctggagcgca ggcacatagg aggaggaaag gaagttgtca
     1201 ctggcgttgt agatcgtcag gagaacccgc ttcttcgggg ctgcactctg cttgatgccg
     1261 gcgtctcggc ttccttcggt atactgtttc cagttggtgg aatcaacacc ggagttggcg
     1321 gccatgaccg ctttga
//
LOCUS       DAOPUZ010000390         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_116416_length_1328_cov_2.582090, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000390 DAOPUZ010000000
VERSION     DAOPUZ010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116416_length_1328_cov_2.582090"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..835
                     /locus_tag="PKN79_06290"
     CDS             137..835
                     /locus_tag="PKN79_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ95406.1"
                     /translation="MANSFECVHLSSITKSFPATQAGDSRLMILEGISLVIKHGQSVA
                     IVGKSGSGKSTLLEIAAGLLEADSGTVMLDGVLLHDLDDRKKAIFRAQKMGFIFQASY
                     MLADFTALENVKIAALIAGSSPQEAHVAATDLLHRVGLEKRLHHTSDKLSGGERQRVV
                     IARSLVNNPAIIFADEPTGNLDEENAAMIEELLLGLVKERNATLLLVTHNPQFASKLD
                     RVLTLRERTLVEGM"
     gene            832..>1328
                     /locus_tag="PKN79_06295"
     CDS             832..>1328
                     /locus_tag="PKN79_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ95407.1"
                     /translation="MSKEVAALLIRRSLGMVGNRASRRRILFQILLITLSLSALVFAL
                     IFVTSMSDAIANKYALINSGHLIVHDRSFPTSGSYDSYLVGTTYGLLYSKETTALVFL
                     KGVEDDYLTGKRKAELVTLKESGESTTLMKVMISKSLAQTLNVHPGENLALMIGTEAN
                     VRPALV"
BASE COUNT          298 a          345 c          376 g          309 t
ORIGIN      
        1 ggtctcgatc tgacccttgc tcttcggtcg ggggaggtcg gtttcttgtg tgttctcatc
       61 cttgcgcttg cggcttttgg cgcctatacg ggttccaggc gcctgctgca cgttgacatc
      121 atggagatac ttggtcatgg cgaactcctt tgaatgtgtc cacctttctt caatcacgaa
      181 atcatttcct gcaacgcagg ccggcgacag ccgcctgatg attcttgaag gaatctcatt
      241 ggtgatcaag cacgggcaaa gcgtggcgat cgtcggtaag agcggctcgg gaaaatcaac
      301 gcttcttgaa atcgctgccg gcctgcttga agcggacagc gggacggtca tgttggacgg
      361 ggtgctcctc cacgaccttg atgatcgaaa gaaggcgatt ttccgggcac aaaagatggg
      421 gttcatcttt caggcaagct atatgcttgc cgatttcaca gcgttggaga acgtaaagat
      481 cgccgccctg atcgccggct cttcccctca ggaagcacat gtagcggcaa ccgaccttct
      541 tcatcgggtg ggccttgaga agcggctcca ccataccagc gacaagcttt cgggagggga
      601 gcgccagcgc gtcgtcatag cacgctcgtt ggtgaacaat ccggcgatca tctttgcaga
      661 tgaaccgacc gggaatctgg atgaggaaaa tgcggcgatg atcgaagagc ttctcctcgg
      721 tctggtgaag gaacgaaacg cgacgctgct gctggttacc cacaacccgc agttcgcaag
      781 caagcttgac cgtgttctca ccttgcgcga gcgaacgctg gtggagggaa tgtgagcaaa
      841 gaggtcgccg ctctcttaat aagacgttct ctcggcatgg tcggaaacag ggcaagcagg
      901 aggaggattc tcttccagat cttgctgatc accctttctt taagcgcctt ggtcttcgcg
      961 ttgatcttcg tcacctcgat gagcgatgca attgcaaata aatatgcgct catcaacagc
     1021 ggccatctca tcgtccacga ccggtcattt cccacgagcg gttcatacga ttcatatctt
     1081 gtcggaacga cgtatgggtt gctttactcc aaggagacga ccgctttggt ctttctcaaa
     1141 ggtgtcgagg atgattatct gacgggaaaa cgaaaagctg agcttgtgac tctaaaggag
     1201 agcggtgaaa gcacaacctt gatgaaagtg atgatcagca aaagccttgc ccaaaccttg
     1261 aacgttcacc cgggggagaa ccttgcactg atgatcggga cggaagcgaa cgtccgcccg
     1321 gcgttggt
//
LOCUS       DAOPUZ010000391         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_117083_length_1323_cov_3.455047, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000391 DAOPUZ010000000
VERSION     DAOPUZ010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117083_length_1323_cov_3.455047"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..>1323
                     /locus_tag="PKN79_06300"
     CDS             162..>1323
                     /locus_tag="PKN79_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNZ95408.1"
                     /translation="MKKRVTIPEHEYFERIAKAAKLTGEAGLDILISNCTEADYSFVR
                     YFTNFWPLFETAGVAIAPNGKAALMVGPESTKFAGDRSVLKTIFTLLEYRESADPAYP
                     EFKPQTFKDVFKHLGVTGKKLRIGIAGYLLTNPIQLEGLRSNFPDAEIVRADDIVRQL
                     RVIKSENELACMREGFRIVELATKEVLKAIRPGVTELQMVGVAQKTIYEHGAEYEGLP
                     MYVFSEKSTSHAISRSTYREIGKGDLVQLNLSAKIEGYSPSVGMPISMGPLVGEKREL
                     VEFGLDMHRWTYKQLKAGVEASSIAQGYYQRFIDLGRKDNFVYGPCHGTGMIEVEAPW
                     MESSSTYKLQPNMTFQVDTFASGPSYGLRWETGVAITEDGYDLLSNPIGEIYE"
BASE COUNT          328 a          374 c          357 g          264 t
ORIGIN      
        1 ggtcgaggtt gagatcgata ccggagcggt attcgtcgac ctctccaacc tgcacgatga
       61 ccacgtccaa cagatcattt atcagtaagc tgtaatcgtc tttcgtggag gattgagcct
      121 cgcccgatcc tccacaccat aaaactacca aggagtacac tttgaaaaaa cgcgtaacaa
      181 tcccggaaca tgaatacttc gaaagaattg caaaagccgc caaactcacc ggtgaggcgg
      241 gacttgacat cctgatctcc aactgcaccg aagcggatta ttcattcgtt cgctatttca
      301 cgaacttctg gcctctgttc gagacagccg gcgtcgcaat cgcccccaat gggaaagctg
      361 ctttgatggt gggaccggaa agcaccaaat tcgccggaga ccgaagtgtc ctcaagacaa
      421 tctttaccct cctcgaatat cgcgagtcag ccgacccggc ctatcccgag ttcaaacccc
      481 agaccttcaa ggatgtcttc aagcaccttg gcgtcaccgg caagaaactg aggatcggaa
      541 ttgcagggta tcttttgacg aaccccatcc agctcgaggg attgcgatcg aacttccccg
      601 atgcggaaat cgtccgggcg gatgacatcg tccggcagct tcgggtcatc aagagcgaga
      661 atgagctcgc ctgcatgcgc gaggggttca gaatcgttga acttgcgacc aaagaggttc
      721 tcaaggcgat ccgcccgggc gtgaccgagc ttcagatggt cggcgtcgcc cagaagacga
      781 tctatgagca cggggccgag tatgaaggac tgccgatgta tgtgttcagc gagaaatcga
      841 ccagccacgc catcagccgt tccacctacc gcgaaatcgg caagggggac cttgtccagc
      901 tgaacctctc cgccaagatc gaaggataca gcccgagcgt cgggatgccg atcagcatgg
      961 gaccccttgt aggcgagaag cgggagttgg tcgaatttgg ccttgatatg caccgatgga
     1021 cctacaaaca gctgaaggcc ggcgtcgaag cctcttcaat cgctcaaggg tactatcagc
     1081 gcttcatcga cttgggcaga aaggacaact tcgtctatgg cccatgccat ggaacgggga
     1141 tgatcgaagt cgaggcgccc tggatggaat cctcttcgac gtacaaactg cagccgaaca
     1201 tgaccttcca ggtcgatacg ttcgcatcgg gaccttccta cggacttcgc tgggagaccg
     1261 gagtcgcgat caccgaagac gggtatgatc tgctctccaa cccgatcggc gagatttatg
     1321 aga
//
LOCUS       DAOPUZ010000392         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_117350_length_1321_cov_3.037125, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000392 DAOPUZ010000000
VERSION     DAOPUZ010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117350_length_1321_cov_3.037125"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PKN79_06305"
     CDS             complement(<1..1034)
                     /locus_tag="PKN79_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ95409.1"
                     /translation="MDIQFYGANRYVTGSCTLVTGNGKKILIDCGLPQGNDAKIGGLQ
                     LPFNAKDIDYLLLTHAHIDHSGRIPLLIKDGFRGTIYATEATKELCSIMLADSAHIQE
                     SEAQWQNRKAKRSGQALVEPLYTVNDAYKSLSRFETIEYEEKRTIDEGITIRYQDAGH
                     LLGSALIELWLTEGAETRKLVFTGDLGNLDQPLIKDPVYITEADFVVMESTYGNRNHA
                     PMIDKDGNTITSLYRARELAAIIQRTFDRGGNVIIPAFSVGRTQELLYLLRIIIEEGL
                     CSETVPVFVDSPLSVKATKVFAGNLYGYMDEETMALVNAGINPILFEGLTPVVDVNDS
                     IALNHRKESCV"
BASE COUNT          314 a          352 c          337 g          318 t
ORIGIN      
        1 acacagcttt ccttgcggtg gttcaaggct attgaatcat tcacatcaac gaccggagtc
       61 aacccttcaa aaagaattgg attgattccc gcattcacca aggccatcgt ctcctcatcc
      121 atgtacccgt acaggtttcc tgcaaaaacc ttggtcgcct tgacggaaag cggactgtcg
      181 acgaagacgg gaaccgtttc ggaacaaagc ccttcttcga tgatgatcct cagcagatag
      241 aggagctctt gggtgcgccc cacgctgaag gcggggatga tgacgtttcc accgcgatcg
      301 aaggttcgct ggatgatcgc tgcgagttcc ctcgcccgat acagcgaggt gatcgtattg
      361 ccatccttgt cgatcatcgg agcgtgattc ctgttcccgt aggtggattc catgaccaca
      421 aaatccgctt cggtgatgta caccgggtct ttgatgaggg gttggtccag atttcccaga
      481 tccccggtga aaacgagctt gcgggtctcg gccccctcgg tcagccacag ctcgatgagc
      541 gccgagccga ggaggtggcc tgcatcctga tagcgtatgg tgattccttc gtcgatcgtc
      601 cgcttttctt catattcgat cgtttcgaac cgggaaaggc tcttatatgc gtcattcacc
      661 gtatacagcg gttcaacaag tgcctggccg ctccgtttgg cttttctgtt ttgccactga
      721 gcttccgatt cctggatatg agcagaatcg gcgagcatga tcgaacacag ctctttcgtc
      781 gcttcggtgg cgtaaatggt ccctcgaaac ccgtccttga tcaacagcgg aatccgcccg
      841 ctatgatcga tgtgcgcatg ggtgagaagg agataatcga tatctttcgc gttgaagggt
      901 agttgaagcc ccccgatctt cgcatcgttt ccctgaggca ggccgcaatc gatcagaatt
      961 ttctttccgt tgcccgtaac cagagtgcag ctgcccgtca catatcggtt cgctccataa
     1021 aattgaatat ccatagttga ctccttgcct tataggatac accgtatcat ggttcgaaac
     1081 ccattgtaaa aatctctgaa aaggaagaaa acttgcaagg cgacggaaga acagggtaca
     1141 cttgagaagg aggatactct tatggatgaa catctctata cgattagaat gaaatcggtc
     1201 caacggacca ttgagcagct caggaaaaac aacatgcagg cgcacttcat ccccaccatc
     1261 gcacaggtga aaaccgagat gaaagcccgt ctctcgaagg gagccacggt agccgtcgga
     1321 g
//
LOCUS       DAOPUZ010000393         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_118967_length_1309_cov_4.045455, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000393 DAOPUZ010000000
VERSION     DAOPUZ010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118967_length_1309_cov_4.045455"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PKN79_06310"
     CDS             <1..539
                     /locus_tag="PKN79_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HNZ95410.1"
                     /translation="TFVIGALNAVSEKLIRRHPHVFSSASAENPDDVLILWDQMKVKE
                     GKQPDEDDFFSRIPPALPIFEKAAEIQHRMRKVGFDWPDASGVFEKVREEIREVRESL
                     EEGGDTEEELGDLLFSVINLTRFLGYNPSYALRRTNKKVQARFNRVVAQAREEGLELK
                     TASLDQLEALWQRAKGEA"
     gene            complement(523..>1309)
                     /locus_tag="PKN79_06315"
     CDS             complement(523..>1309)
                     /locus_tag="PKN79_06315"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNZ95411.1"
                     /translation="FLIEEYKRASRLLLTGRCTAECAHRCAACTSAHKVVDAKRSDAV
                     EYPKPQERPSPLSYVQALVTYRRVFPATMISHINTMRNVEMALQRANLQVQFTQGYNP
                     TPKLEFVNPLSLGITGEAEILLIELVDDPTLTEAGVKEALQEKLHDGFMITDVVFLPK
                     GKKQTLARHLVASRYEFDTKGSIEAQSALRALVREPKEGATVKEIREDRFEVIIHGER
                     NMIKLLFGKDADKFLIASKYGITRTHLYAGEIGTSYSEFMLRL"
BASE COUNT          291 a          344 c          364 g          310 t
ORIGIN      
        1 acacgttcgt gatcggggcg ttgaatgccg tcagtgaaaa gctcattcgg cgtcaccctc
       61 atgtcttttc ctccgcttcg gcggagaatc ctgatgatgt cctcatactt tgggatcaaa
      121 tgaaggtcaa agaaggaaag cagccggatg aagacgactt cttcagtcgc attccccctg
      181 ctcttcccat cttcgaaaaa gccgctgaaa tccagcatag aatgagaaag gtcggtttcg
      241 attggccgga tgcttccgga gtcttcgaga aggtccggga agaaattcgg gaagtgcgtg
      301 aatcattgga ggaaggcggc gataccgagg aagaactggg ggatcttctc ttcagcgtca
      361 tcaatcttac tcggtttctg ggctacaacc cctcctatgc gcttcgtcgg acaaataaaa
      421 aggtgcaggc ccggttcaac cgggtagtcg cccaagcacg ggaagaaggt ttggaactca
      481 aaacggcgag tctcgatcag ttggaagcgt tatggcagcg agctaaaggc gaagcatgaa
      541 ctcgctgtag gaggttccga tctcccccgc atagaggtgc gttcgggtta ttccatattt
      601 ggaagcgatc aggaacttgt ccgcatcctt tccgaagagc agcttgatca tattgcgttc
      661 gccatggatg ataacctcaa agcgatcttc tcggatctct ttcaccgtgg cgccttcttt
      721 gggctcccga accagtgctc gaagagcgct ttgagcctca atggatcctt ttgtatcgaa
      781 ctcatagcgg ctggcgacga ggtgccgggc aagggtttgc ttcttccctt tcgggagaaa
      841 cacgacatcc gtgatcataa aaccatcatg gagtttttcc tgcagggctt cttttacgcc
      901 cgcttcggtg agagtcggat catcaaccag ctcgatcaga aggatttccg cctcccccgt
      961 tattccgagg gaaagcggat tcacgaactc aagcttgggc gtcgggttgt acccttgggt
     1021 gaactgcacc tgcaggtttg cccgctgaag cgccatctcg acgtttctca tcgtattgat
     1081 gtggctgatc atggtcgcgg gaaagacccg tctataggta acgagcgcct gcacatagga
     1141 tagcgggctc ggccgctcct gcggtttcgg gtattcgacg gcatccgacc gtttggcatc
     1201 aaccacctta tgggcggaag tacatgccgc acaccgatga gcacactcgg cggtgcaacg
     1261 gccggtaagc aggagcctgc tcgcccgttt atactcctcg atgaggaac
//
LOCUS       DAOPUZ010000394         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_119299_length_1307_cov_2.670927, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000394 DAOPUZ010000000
VERSION     DAOPUZ010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119299_length_1307_cov_2.670927"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PKN79_06320"
     CDS             <1..942
                     /locus_tag="PKN79_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ95412.1"
                     /translation="KGVSDETRTRILEIARRMNYRLPSERIVHQESKGFIMFARLSKH
                     GLILNRDQSSFIVDYIDSINDAVTKAGYTFEILDHTFTSVSTLTELLSSRKPKGIIVL
                     GTELDEDDVHNLQSLPFTFVVIDTQFESACCDFVDMANIAALTKIVNHLIDRGHKRIG
                     MISCTTPSGNIILRERGFSLALERSGLSPASFLRIQPGFKGAYNDMKTYLKQENQLPE
                     AFFCYNDVAALGVMKALKEHRIRIPEDVSIIGFDNLPMSSMSEPRLTTIKVPNRLIGT
                     IATELLVKKLSTKHQKESITIQVNGTLVVRDSVMDRR"
     gene            958..>1307
                     /locus_tag="PKN79_06325"
     CDS             958..>1307
                     /locus_tag="PKN79_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ95413.1"
                     /translation="MSNYSIILGNLGNTRDRFLSSGYKDELPKETLIRQASEINGVTG
                     VELVGTWDVTSENVEQVGEMLDKHGLKCVSIIPDHFAQKRWGRGTLSAKDEDIRKQAL
                     DYTFECVEMARTLG"
BASE COUNT          337 a          342 c          345 g          283 t
ORIGIN      
        1 aagggcgtca gcgatgaaac tcggacaaga atcctggaga tcgcccgcag gatgaactac
       61 cgcctcccct ccgaacgaat cgttcatcag gaaagcaagg ggttcatcat gttcgcccga
      121 ctcagcaaac atggcttgat cctcaatcgc gatcaaagca gcttcatcgt ggattacatc
      181 gattcgatca acgatgccgt aacgaaagcg ggctatacct ttgaaatcct cgatcacacc
      241 ttcacctccg tttccacatt gacggagctt ctttcctctc gaaaaccgaa aggaatcatc
      301 gtgctgggta ccgagctgga tgaggatgac gttcacaacc ttcaatcatt gccgttcacg
      361 tttgtcgtga tcgatactca atttgaatcg gcgtgctgcg actttgtcga tatggcgaat
      421 atcgcggcgc tgactaagat cgtcaaccac ctcatcgatc gcggacacaa aaggatcggg
      481 atgatcagct gcaccacgcc cagcggaaac atcatcctgc gcgaacgggg attttcgctg
      541 gcgttggagc gatccggcct ttctccggca tcctttctgc gaatccaacc gggattcaaa
      601 ggtgcgtaca acgatatgaa gacgtacctc aaacaggaga atcagttgcc ggaggcgttc
      661 ttctgctaca acgacgtggc ggccttggga gtgatgaagg cgttgaagga acacaggatt
      721 cgaatccccg aggatgtttc gatcatcggc ttcgacaatc ttccgatgag ttcgatgtcc
      781 gaaccccggc tgaccacgat caaggtcccc aaccgcctca tcggcaccat tgcaaccgaa
      841 ctgctcgtta aaaaactttc aacaaagcat caaaaggagt cgatcacgat ccaagtgaat
      901 ggaacgctgg tcgttcgtga cagtgttatg gatagaagat gacaaggagg tattgtcgtg
      961 agcaactatt caattatttt aggcaatctc gggaacaccc gggatagatt tctttcgtca
     1021 gggtacaagg acgagcttcc aaaggaaaca ctgattcgtc aggcttcgga aatcaacgga
     1081 gttaccggag tcgagctcgt gggaacatgg gatgtgacct cggagaacgt cgagcaggtc
     1141 ggagagatgc tggacaagca tggcctgaag tgcgtctcga tcatccccga ccacttcgcc
     1201 cagaagcgct ggggacgcgg tacgctttcc gccaaggatg aggatatccg gaagcaagcg
     1261 ctcgactaca cctttgaatg cgtcgagatg gctcgaacgc tcgggtg
//
LOCUS       DAOPUZ010000395         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_119315_length_1307_cov_2.428914, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000395 DAOPUZ010000000
VERSION     DAOPUZ010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119315_length_1307_cov_2.428914"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKN79_06330"
     CDS             complement(<1..398)
                     /locus_tag="PKN79_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ95414.1"
                     /translation="MQTPPMIGKNIQRIRTARKLTLNVLSERSGVSKAMLSQIESDKV
                     NPTVATVWKIAKGLNVELNDLLEGVDEPTRVFSLNPASDDGSKLEKRKDGITIKILSP
                     LSSVEDLEMYLLTFEPNSTLASEPHVAGSQ"
     gene            513..>1307
                     /locus_tag="PKN79_06335"
     CDS             513..>1307
                     /locus_tag="PKN79_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ95415.1"
                     /translation="MKRILIIGALGQLGSEIALECRRRYGTDNVVLADIRSDVNRPLI
                     EDGPFYLLDARDGKRIDEVVRTHRIDTIYHLAALLSARAEQDPMEAWNLNINGLIAVL
                     EVARQKSCAVFVPSSIGAFGPTTPKKFTPQDTIQRPTSIYGVTKVTGELLCDYYHHRW
                     DVDTRGIRFPGIISSLTPPGGGTTDYAVEIYYEAIRNQHYTCFLRGDTYLDMIYMPDA
                     VEAAIQIMEADPGRLRHRNAFNIAAMSFCPEEQAAYIRTHIPNFTIS"
BASE COUNT          297 a          364 c          338 g          308 t
ORIGIN      
        1 tcctggctac cggcgacatg aggctcgctt gcaagggtcg agttcggttc gaaggtaagg
       61 agatacatct ccagatcttc cacggaagac agggggctga ggattttgat cgtgatcccg
      121 tccttccgct tctcgagctt cgatccgtca tcggaagcgg gattgagcga aaacaccctt
      181 gtcggttcat caactccttc caaaaggtcg ttcaattcga cattcagccc tttggcgatc
      241 ttccataccg tggcaacggt gggattcacc ttgtcgcttt cgatttgact gagcatcgct
      301 ttggagacac cgcttcgctc actcaagaca ttgagggtaa gcttgcgagc cgttcgaatt
      361 cgctgaatgt ttttaccgat catcggaggg gtttgcatcg ggaactcctt ataaagtttg
      421 acatactaaa ccctgttcaa tatactgaac ctgcttacag attgtaaaag cacgctccgt
      481 tcctgtcaag aaacaagcgg aagcgaggag aaatgaagcg catattgatc atcggtgcac
      541 tcggtcagct cggcagtgaa atcgctctcg aatgtcgccg tcgctatgga accgacaacg
      601 tcgtattggc tgatatcagg agcgacgtga atcgccctct gatcgaagat ggtccgtttt
      661 atcttcttga tgcccgcgac gggaaacgga tcgacgaggt cgttcgcacc caccggatcg
      721 atacgatcta ccatttggca gccctgctct ccgcccgtgc cgaacaggac cccatggaag
      781 cgtggaatct caatatcaac ggcctgatcg ccgtgctgga ggtcgcccga cagaagtcgt
      841 gcgccgtctt tgttccctct tcgatcggcg cgttcgggcc gacgacccct aaaaaattca
      901 cccctcagga tacaattcaa cgccctactt cgatctacgg tgtcaccaag gtgaccgggg
      961 agctgctctg cgactattac caccaccgct gggatgtcga taccagaggg attcgcttcc
     1021 ccggcatcat cagcagcctg acgcctccag gtggcggaac taccgattat gcggtggaga
     1081 tctactacga agcgatccgg aatcagcact atacgtgctt tctccgcggc gatacctacc
     1141 ttgacatgat ttacatgcct gatgcggtcg aggcggcgat tcagattatg gaagcggatc
     1201 caggcagact tcgacatagg aatgctttca atatcgccgc gatgagcttc tgtccggaag
     1261 agcaggcggc gtatatccgc acccatattc caaacttcac gatcagc
//
LOCUS       DAOPUZ010000396         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_119995_length_1302_cov_3.412991, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000396 DAOPUZ010000000
VERSION     DAOPUZ010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119995_length_1302_cov_3.412991"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKN79_06340"
     CDS             complement(<1..210)
                     /locus_tag="PKN79_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNZ95416.1"
                     /translation="MAEVTLEKIVSLSKRRGFVFQSSEIYGGLNGAYDYGPLGVQLKN
                     NIRDLWWKEMTQLHDDIVGLDAAILM"
     gene            complement(212..>1302)
                     /gene="gltX"
                     /locus_tag="PKN79_06345"
     CDS             complement(212..>1302)
                     /gene="gltX"
                     /locus_tag="PKN79_06345"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNZ95417.1"
                     /translation="QGYDRHCRNLSSEERLAYEEQGIRPVIRLKVPTEGKTSFEDILM
                     GTITRRNSDVSPDPVLLKSDGFPTYHLANVIDDHMMEITHIMRAQEWIPSGPLHILLY
                     EAFGWEPPKYCHLPMVMGKDGQKLSKRHGSTAVAEFRKEGYLPEALLNYVSLVGWSYD
                     GTREFFTKEELEELFSLEKINKAPGVFDYKKLDWFNGQYIRMKSDEELLDLLMPYLAE
                     TGWPLTDRAKLLHLTAVAKERLKVLSDITGLARFLFEEPAWDDLSIFAAKGVELPLAH
                     TALERAYTVLLSGLEAKKEQSVIEEELAQLAKDLGMKVNGVFQPLRVAITGSTVSLPL
                     FDSIELLGFDHTMNRIQDALNVLKKGIE"
BASE COUNT          247 a          383 c          363 g          309 t
ORIGIN      
        1 catgaggatc gcggcgtcca ggccgacgat gtcgtcgtgg agctgggtca tctccttcca
       61 ccacagatcg cggatattgt ttttcagctg cactccgagc ggtccgtaat cgtaggcgcc
      121 gttcaagccg ccgtagattt cgctcgattg gaagacaaat ccccgtcgct tgctgagtga
      181 tacgattttc tctaacgtaa cttctgccat cttattcaat tcctttcttc agcacgttca
      241 gtgcgtcttg tattcggttc atcgtgtggt caaagccgag cagctcgatc gagtcgaaga
      301 ggggcagact gaccgtgctg ccggtgatcg cgacccgaag gggctgaaac accccgttga
      361 ccttcatccc gagatccttc gccaattggg cgagctcctc ttcaatcacg ctttgctctt
      421 tctttgcttc caagccgcta aggagcactg tataagcacg ctcaagggcg gtatgggcca
      481 acggaagctc gacgcccttc gcggcgaaga ttgaaaggtc atcccacgcg ggctcttcaa
      541 agagaaaacg agccaggccg gtgatgtcgc tgaggacttt cagccgctct ttcgcaaccg
      601 cggtcagatg cagcagcttc gctcggtcgg tcaagggcca ccccgtctcc gccagatacg
      661 gcatcaacag atccaagagc tcctcatcgc tcttcatgcg gatgtattgg ccgttgaacc
      721 agtcgagctt cttgtagtcg aagactccgg gagccttgtt gatcttctcc aggctgaaga
      781 gctcttccag ctcctccttc gtgaagaact cccgggttcc gtcgtacgac cagccgacga
      841 gggacacgta gttcaggagc gcttcgggaa gatagccttc cttccggaac tccgccaccg
      901 cggtcgaacc atggcgtttg gagagcttct gcccgtcctt gcccatcacc atcggcaggt
      961 ggcagtactt cggcggctcc cagccgaacg cttcatagag gaggatgtgc aagggtccgg
     1021 aggggatcca ctcttgggcg cgcatgatgt gggtgatctc catcatgtgg tcatcgatga
     1081 cgttcgcaag gtggtaggtc gggaacccgt cgctctttaa cagcaccggg tcgggggaga
     1141 catcgctgtt cctgcgggtg atcgtaccca tcaggatgtc ttcaaagctc gtctttccct
     1201 cggtggggac tttgaggcgg atgaccggcc tgatcccctg ttcttcatac gccaagcgct
     1261 cttcactgct taagttcctg cagtggcggt catatccctg at
//
LOCUS       DAOPUZ010000397         1297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_120721_length_1297_cov_2.528180, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000397 DAOPUZ010000000
VERSION     DAOPUZ010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120721_length_1297_cov_2.528180"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="PKN79_06350"
     CDS             complement(<1..1164)
                     /locus_tag="PKN79_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNZ95418.1"
                     /translation="MKRFAALLSIVFLSTSLLFAEEPTVALVLSGGGARGMAHIAVLE
                     ALEEEGIPIDLIVGTSIGALIGGMYSVGYTPNEIREILFNLDMIELFSEAVTEEGGTL
                     DRAFVPDRNHVFNLRFSGSSIGKTPAIIGDQRILELLGFLFAKYPNPIDFDELPIPFR
                     AISSDIMSRDEIVHDFGSLVRAIRASISIPLIFAPFPLEDGRLAVDGGAVDNLPINVA
                     RALGADYVIASDLNAFINEDKNQAETLSGVVIHSLSLVIQRSAFAQHEDADLLFTSDL
                     HDISIMDAFKLEAIEQRGREAVERNRPQLQALRNRLKTERDLIVRDPHRVGSYALKPD
                     PIIRSVAIENISLTPKPLFFDESLFTFMVGSPLDKEHAAELALRLRQVRKQNSL"
     gene            complement(1167..1286)
                     /locus_tag="PKN79_06355"
     CDS             complement(1167..1286)
                     /locus_tag="PKN79_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95419.1"
                     /translation="MGTGLGVLWVTTLQLEKRKEMDWRSFINGNQRFIGSRLE"
BASE COUNT          303 a          393 c          348 g          253 t
ORIGIN      
        1 taaagaattc tgctttcgaa cctgccgcaa gcggagcgcc aactccgccg catgctcctt
       61 gtcaagcgga gaaccgacca taaaggtgaa gagcgactca tcgaagaaga gcggcttggg
      121 agtcagggag atattttcga ttgcgacgga gcggatgatc ggatccggct tcagggcgta
      181 cgagccgacc cgatgcggat cccggactat caaatcgcgc tccgttttca gccggtttcg
      241 cagcgcttga agctgaggac ggtttcgctc caccgcctcc ctgccccgct gctcaatcgc
      301 ctccagcttg aacgcatcca tgatggagat atcgtgaaga tcgctcgtga acaagaggtc
      361 ggcatcttca tgctgggcga atgcgctgcg ctgaatcacc agggaaagcg aatggatcac
      421 cacccccgaa agcgtttcag cctggttttt gtcctcgttg atgaacgcgt tgagatcgct
      481 ggcgatcacg taatccgccc cgagcgcccg ggcgacgttg atcggcaggt tgtccaccgc
      541 tccgccgtcg acggcaagcc gcccgtcctc caaaggaaac ggtgcgaaga tgaggggaat
      601 cgagatgctc gcccggatcg cccggaccag agagccaaag tcgtgaacga tctcatccct
      661 gctcatgata tcggaggaaa tcgcccggaa cggaatcggc agctcgtcaa aatcaatcgg
      721 gttgggatat tttgcaaaga ggaacccgag cagctccaaa atccgctgat ccccgatgat
      781 cgccggagtt ttgccgatcg acgaaccgga aaagcgcaga ttgaacacat ggttccgatc
      841 ggggacgaac gcacggtcga gggtgccgcc ctcttcggtc accgcttcgc tgaacagctc
      901 gatcatgtca aggttgaaga ggatctctcg aatctcattt ggagtgtacc cgacgctgta
      961 catccctccg atcagcgccc cgatcgacgt gccgacgatc agatcgatcg gaatcccctc
     1021 ctcctcgagg gcctccaaga cggcgatatg ggccattccc cgcgcccctc ctcccgagag
     1081 gaccaacgcg acggtcggtt cttcagcgaa aagcaacgaa gtcgacaaaa acacgatgct
     1141 cagaagagcg gcaaatcgtt tcatacctac tccaatcgcg aaccgataaa acgctgatta
     1201 ccattgatga acgaacgcca atccatttcc tttctttttt caagctgcag ggtcgttacc
     1261 cacagaactc ccagacccgt tccgatcccg atccctt
//
LOCUS       DAOPUZ010000398         1297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_120728_length_1297_cov_2.439614, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000398 DAOPUZ010000000
VERSION     DAOPUZ010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120728_length_1297_cov_2.439614"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1297
                     /locus_tag="PKN79_06360"
     CDS             <1..>1297
                     /locus_tag="PKN79_06360"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HNZ95420.1"
                     /translation="AGGELTFTNYGAYLAANPAKEWAVLHDGEEKKGSSWSCSHGVSR
                     WYKDCGCYTGGNDSWNQKWRTPLRASFDALAKRIDEIYISEASKMLGSEETARKVLNS
                     FAPVASHLVPLHEFLAPYTTDEEEITTLGCLLQGQKYKHYSYTSCGWFFNDLAGIEPK
                     QNIIYALMAAKLYESYDPLLTSTLLKNLEKAKANEKEDGNGKTIALELMKLLPGKVEA
                     ALYYILNRTLALPSDQQDHYGFFRLDRFKEEEGLFALSITNEQCLQKHQITARMGQGK
                     YSLTVDSKPYSLTASEIPHRMRRELLEQIDKRICTVDEDQIIRLDQSLIHYSLLAQSA
                     PEVLEPVYQQLVGWAISMIKGLFLFERIRFWEHYRERFLRVLDFFTVYGKAEEIQLLG
                     ELFDQVLFNVAEKIQRNGLFEQSIRFVSEFLEEVRKRGFQ"
BASE COUNT          324 a          341 c          340 g          292 t
ORIGIN      
        1 agcgggcggg gaattgacgt ttaccaacta cggcgcctat ctggccgcca atccggcgaa
       61 ggagtgggcg gtcctgcatg atggtgaaga gaaaaaagga tcttcctggt cgtgctccca
      121 cggtgtttcc cggtggtata aggattgcgg atgttatacc gggggaaacg attcgtggaa
      181 ccaaaaatgg cggacccccc ttcgcgcctc gtttgatgcg cttgccaaac gaatcgatga
      241 gatctatatc agtgaagcaa gcaagatgct tggcagtgaa gaaaccgccc ggaaggttct
      301 gaacagcttt gctccggtcg cttctcatct cgttcccttg catgaattcc ttgcaccgta
      361 taccaccgac gaagaggaga tcacgaccct tgggtgcctg cttcaagggc aaaagtataa
      421 acactacagc tataccagct gcggatggtt tttcaacgac ctggcgggca ttgaaccaaa
      481 gcaaaacatc atctatgccc tgatggcggc aaaactctac gaatcgtatg atccgctgct
      541 cacctccacc ctcttgaaaa accttgagaa ggcgaaggcg aacgaaaaag aggatggcaa
      601 cggaaaaacc atcgcccttg aactgatgaa gcttctgccc ggcaaagttg aggcggcatt
      661 gtattatatt ctgaaccgaa ccctcgccct gccgagcgat cagcaggatc actatgggtt
      721 cttccgcctc gaccggttca aggaggaaga agggctcttc gccttgtcga tcaccaatga
      781 acagtgtctg caaaaacatc agatcaccgc tcggatgggg caaggcaagt attcgctgac
      841 ggttgattcc aaaccctact cgcttaccgc ctcggagatt cctcatcgaa tgcgccggga
      901 gcttttggag cagatcgaca agcggatctg taccgtcgat gaggatcaga tcatccgcct
      961 cgaccaaagc ctgatccatt acagccttct ggctcaaagc gcccccgagg tgcttgagcc
     1021 ggtgtatcaa cagcttgtcg gatgggcgat cagcatgatc aagggactct tcctctttga
     1081 gcgaatccgc ttctgggaac attacaggga gcgcttttta cgggtcttgg atttcttcac
     1141 cgtctatgga aaagccgaag agattcaact gcttggagaa ctgttcgatc aagtactctt
     1201 taacgtcgct gaaaagattc agcgcaatgg actcttcgaa cagtcgatcc gcttcgtttc
     1261 cgaatttctc gaagaggtgc gcaagcgggg cttccaa
//
LOCUS       DAOPUZ010000399         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_120827_length_1296_cov_3.414182, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000399 DAOPUZ010000000
VERSION     DAOPUZ010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120827_length_1296_cov_3.414182"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKN79_06365"
     CDS             complement(<1..590)
                     /locus_tag="PKN79_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HNZ95421.1"
                     /translation="MRTFPITGRIKRFASVFRDNGYSLFVVGGAVRDFLLGLGNSDLD
                     FTTDARPEEVIALFRTVIPTGIAHGTVTVIFEGEHYEVTTFRSEGEYKDGRRPSSVTF
                     IPSLEEDLKRRDFTINAFAASAITGKIIDNHNGEQDLKDGTIRAIGNPYERFDEDALR
                     IMRAARISGKLNFTIEDQTFNAMGALKENLRLVSVER"
     gene            709..>1296
                     /locus_tag="PKN79_06370"
     CDS             709..>1296
                     /locus_tag="PKN79_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014696647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNZ95422.1"
                     /translation="MFRPVNPKVDFPKMEEGVLQFWEERNIFKKSIEYRPEEKEYVFY
                     DGPPFATGLPHFGHIVPGTIKDTIPRYQTMKGRRVNRRFGWDCHGLPVEYEIEKSEGI
                     SGYSAIVEFGVARFNEMCRSIVLRYTKEWEITIKRTGRWVDWEDSYRTMDLSYMESIW
                     WVFKTLYEKGYIYEGYNILPYSPKLASPLSNFEVNL"
BASE COUNT          322 a          312 c          351 g          311 t
ORIGIN      
        1 cgctcaaccg aaacgagcct gaggttctct ttcaatgcac ccatcgcatt gaaggtttgg
       61 tcttcaatcg tgaaattgag ttttccgctg attcgagcgg ccctcatgat cctcagggca
      121 tcttcatcaa agcgttcgta gggattcccg atcgcccgga tggtcccgtc tttcaggtcc
      181 tgttcaccgt tgtgattatc gatgatcttt ccggtgatcg ccgaagcggc gaatgcattg
      241 atcgtgaagt cacggcgctt caggtcctct tccagagatg gaatgaaggt aacggaagaa
      301 ggtcttcggc catccttgta ttccccctcg cttcggaacg tcgtaacttc gtaatgttcc
      361 ccctcaaaga tgacggtaac ggtcccatgg gcgattcccg tcggaatgac ggttcgaaag
      421 agggcgatca cctcttcggg acgggcatcg gtggtaaagt ccagatcgga gttccccaag
      481 cctaagagaa agtcacgaac cgcaccgccg acaacaaaaa gagagtagcc gttgtcacgg
      541 aagacggagg caaagcgttt tattctgccg gttatcggaa aggttcgcat atgatgacag
      601 tactccctca tcgggatgag gtcaacggtc tattcatggt tgtcatttat gcgatgaatg
      661 aatagaatga cactgataca caaggcccga ttgaggcgag gagcaaatat gtttcgtccc
      721 gttaatccga aagtagattt tcccaagatg gaggaagggg ttcttcagtt ttgggaagag
      781 cggaatatct ttaagaagtc gattgaatac cgcccggaag aaaaagagta cgtcttctat
      841 gacggacctc ccttcgccac cggtcttccg cactttggcc acatcgtgcc cgggacgatc
      901 aaagacacga ttcctcgata tcaaacgatg aagggaaggc gggtgaaccg ccgcttcggg
      961 tgggattgcc acggccttcc ggttgaatat gagatcgaaa agagcgaagg gatcagcggc
     1021 tattcggcaa tcgtcgagtt tggcgtagcc aggttcaatg agatgtgccg ctcaatcgta
     1081 ttacgctata ccaaggagtg ggagatcacg atcaaacgga ccggtcgctg ggtcgattgg
     1141 gaggacagct atcggacgat ggatctctcc tatatggaat cgatctggtg ggtgttcaag
     1201 acgctctatg aaaaaggata tatctacgaa ggatacaata ttcttcccta cagtccgaag
     1261 cttgccagtc cgctttctaa cttcgaggtg aacctc
//
LOCUS       DAOPUZ010000400         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_121429_length_1292_cov_3.218270, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000400 DAOPUZ010000000
VERSION     DAOPUZ010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121429_length_1292_cov_3.218270"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PKN79_06375"
     CDS             <1..970
                     /locus_tag="PKN79_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNZ95423.1"
                     /translation="LNRHSLLHIDMRNFFRDYPQDSHPMSILAAMVASLSAFYPELAD
                     VDPKANLDLTVTRLLSKMRTIAAFTYRQMNGLEFVNPSYKFSYCENFLNMMFYTSVNA
                     YIPPPLHVRALNILLILHADHEQNCSTSAVRLVGSSDANLYASVAAGCCALWGTKHGG
                     ANQAVMQMLLKIHNEGLTIEQVVEMAKNKENGFRLAGFGHRIYKTYDPRAKIAKDLCQ
                     KVIGNTELDPLLQIALELEQVALSDPYFIERNLYPNIDFYTGIIFHAMGIPESMFTVL
                     FALGRLPGWIAHWLEWAGDPYQKIARPRQVYTGLAKRNVVPLADRV"
     gene            complement(993..>1292)
                     /locus_tag="PKN79_06380"
     CDS             complement(993..>1292)
                     /locus_tag="PKN79_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNZ95424.1"
                     /translation="PGANINYQSGHAVFEATHGTAPDIAGRNLVNPLSLVLSSVLMLR
                     HLGWDEAAALLNEAVRKTIRTGIVTKDFYSLIEKEGRSATLLGTKEYGERLCALI"
BASE COUNT          269 a          384 c          334 g          305 t
ORIGIN      
        1 gctcaaccgc cattccctgc tccacatcga tatgcggaac ttcttccgcg actatcctca
       61 ggactcgcac ccgatgtcga ttctggcggc gatggttgct tccctttcgg cgttctatcc
      121 cgagctcgcc gatgtagatc cgaaagcaaa cctcgacctc accgtaacgc gccttctttc
      181 aaagatgcga acaatcgccg cgtttaccta tcggcagatg aacggcttgg agtttgtaaa
      241 cccctcctat aagttctcct actgtgaaaa cttcctgaac atgatgtttt ataccagcgt
      301 aaacgcctat attcctccgc cgctccatgt ccgggcattg aacatccttc tgattcttca
      361 cgccgaccat gagcagaact gctcaacgag cgccgtacgg ctcgtcggtt ccagcgatgc
      421 gaacctctat gcttcggttg ccgccggctg ctgcgccctc tggggaacaa agcatggcgg
      481 agcgaatcag gcggtgatgc agatgctgct gaagattcac aatgaaggct tgacgatcga
      541 acaggtcgtc gaaatggcca agaacaagga aaacggattc cgccttgccg gcttcggcca
      601 ccgaatctat aaaacctacg atccccgggc caagatcgcc aaagatctct gtcagaaggt
      661 aatcggaaac acggagctgg atcccttgct gcagattgca ctggagctgg agcaggtagc
      721 cttgagcgac ccgtatttta tcgaacggaa cctgtatccc aacatcgatt tctatacggg
      781 aatcatcttc catgcgatgg gaatccccga gtcgatgttc accgtcctct ttgccttggg
      841 ccgccttccg ggctggatcg cccactggct tgaatgggcc ggcgacccct atcagaagat
      901 cgcccgcccc cgccaggtgt acaccggtct ggcgaaacga aacgtcgttc ctttggccga
      961 tcgggtatga gcttgaaggt ttcttctttt gtctagatca gcgcgcagag gcgctctccg
     1021 tactcctttg ttccgagcag ggtggcgctt cttccctctt tttcgatcag ggaataaaag
     1081 tccttcgtca cgatcccggt tctgatcgtc tttctgaccg cttcgttgag aagggccgcc
     1141 gcttcatccc accccagatg tcgaagcatc aggacgcttg aaaggacgag cgaaagcgga
     1201 ttgacgagat tcctaccggc gatgtccggg gcggttccgt gggttgcctc aaagaccgcg
     1261 tggccgcttt gatagttgat gttcgccccg gg
//
LOCUS       DAOPUZ010000401         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_121615_length_1291_cov_2.530744, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000401 DAOPUZ010000000
VERSION     DAOPUZ010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121615_length_1291_cov_2.530744"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PKN79_06385"
     CDS             complement(<1..809)
                     /locus_tag="PKN79_06385"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNZ95425.1"
                     /translation="MKRKYWLETYGCQMNIAESNALELHLKGAGLIPAESAEESAVVI
                     LNTCTVRRSADNRIWGRLGYFGALKKRQPLTLVVTGCMAERLKDDLKNDAPYVDFVIG
                     TYDKQRIVDILTSPNGTYDEEETYTFSDSYYREGEFSSYIPIMNGCNNFCSYCIVPYV
                     RGREISRPINDIIRELHYLDSKEVKEVTLLGQNVNSYHYEEDGKVITFPELLRRLCAE
                     DTKHIRWIRFDSPHPKDFTGELIEVIRSEEKVAKHLHIPLQSGADPVLRSMR"
     gene            complement(787..>1291)
                     /locus_tag="PKN79_06390"
     CDS             complement(787..>1291)
                     /locus_tag="PKN79_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNZ95426.1"
                     /translation="LLGNPSLVSCTPLGILKMLEYYEIETSGKHVVVVGRSHIVGKPI
                     AALLFQKGWDATVTICHSKTQNLRDITSLADILIVAIGKPLFITPDYVKEGAVIIDVG
                     INRVEDAGRSRGYRLVGDVDYERVAPKTTAITPVPGGVGVMTVAMLMNNTLQAAKKGR
                     NEAEILA"
BASE COUNT          272 a          359 c          343 g          317 t
ORIGIN      
        1 cgcattgatc gcaaaactgg atccgcccca ctctgcaaag ggatatgcag gtgcttggcc
       61 accttctctt cgcttctgat cacctcgatc agctctcccg tgaaatcctt cggatggggg
      121 ctgtcgaagc ggatccagcg gatatgcttc gtatcctcgg cacagagccg ccgaagcagt
      181 tcggggaatg tgatgacctt cccgtcctct tcgtagtgat agctgttcac attctggcca
      241 agcagggtca cttcctttac ttctttagaa tcaaggtagt ggagctccct gatgatatcg
      301 ttgatggggc gcgagatctc ccgcccccgg acatagggaa cgatgcagta ggagcagaag
      361 ttgttgcagc cgttcatgat cgggatgtag gaggagaact cgccctcccg gtagtatgaa
      421 tcactgaagg tgtaggtctc ctcttcgtcg taagtaccgt tcggagaggt caggatatca
      481 acgatccgct gtttgtcgta ggttccgatg acgaaatcga catatggagc atcgttcttc
      541 aagtcatcct tcaggcgctc ggccatgcat ccggttacga ccaaggtcag cggctgtctt
      601 ttcttcagcg ctccgaaata cccgagcctc ccccaaatcc ggttgtcggc ggatctgcgg
      661 accgtgcacg tattgagaat cacgacggcg gattcttcgg cgctctctgc cggaatgaga
      721 ccggcaccct tcaaatggag ttccaacgcg ttggactcgg cgatgttcat ctggcacccg
      781 taggtctcaa gccagtattt ccgcttcatt gcgccccttt tttgccgctt gtaaggtgtt
      841 gttcatcaac atcgccacgg tcatgactcc aaccccgccg ggaaccggag tgatggcggt
      901 cgttttagga gcgacacgtt catagtcgac atcgccaacc agccgatagc cccgtgaccg
      961 accggcatcc tcaacccgat tgattccgac atcgatgatt accgccccct ctttaacgta
     1021 gtcgggggta atgaagaggg gttttccgat cgcgacgatc aggatgtcgg caaggctcgt
     1081 gatgtcacga aggttttgcg ttttcgagtg acagatggta accgtggcat cccatccctt
     1141 ttggaacaac aatgcggcga tgggctttcc tacaatatgt gagcgaccga ccaccacgac
     1201 gtgttttcct gacgtttcga tctcatagta ctcaagcatc ttcaaaatgc ccaatggtgt
     1261 acagctgacc agggatgggt ttcccaacag a
//
LOCUS       DAOPUZ010000402         1290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_121734_length_1290_cov_3.131984, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000402 DAOPUZ010000000
VERSION     DAOPUZ010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121734_length_1290_cov_3.131984"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..901)
                     /locus_tag="PKN79_06395"
     CDS             complement(53..901)
                     /locus_tag="PKN79_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ95427.1"
                     /translation="MQIRRTFNPINLLIYALLLVMVFFTLTPIVFMVTASMMTKNQIL
                     RMPFNWIPESLNWINFKRAIVGLDENYIFLRNIVNSLIVSVTVAITTVLLGSLTGYGL
                     AKFRFRGRNVIFIMIMATMMIPFEAIMIPLYMVVMKLRIQNTYVGLILPFLVSAFGIF
                     QMRQYLSTFPTEFLDAARVDGMSEPGIYWQIVLPNAIPVIATLSILAFRSQWDNLLWP
                     LLVSQKDKMKTIPQYITSFAAERNTDEGAMMAAALIASIPMFVLFMSLTKYFIGGSAV
                     YESRKG"
     gene            complement(911..>1290)
                     /locus_tag="PKN79_06400"
     CDS             complement(911..>1290)
                     /locus_tag="PKN79_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ95428.1"
                     /translation="YFVILFITGLATIPQTIYEAATIDGANKSQTFWRITLPLLKPTV
                     VLVSIMAMLQCLKTFSTQYMFYTNGSTRAPINVITFNIYITGIQDQYLGRASAMSVVL
                     FVLMLVLTLFQFRTTKSGENIDY"
BASE COUNT          308 a          304 c          386 g          292 t
ORIGIN      
        1 gaaaagaaac acctttgtgt ctttgccggc gattgcattc ctgtccatcg gactatccct
       61 tgcgtgattc gtagaccgcc gatcctccga tgaagtactt cgtgaggctc atgaagagga
      121 cgaacatcgg aatcgaggcg atcagggcgg cggccatcat cgctccttcg tcggtgtttc
      181 gttcagcggc gaatgacgta atatactgag gaatcgtttt cattttatct ttttggctga
      241 ctaaaagagg ccacaggagg ttgtcccact gcgagcggaa cgccagaatt gagagcgtgg
      301 cgatcaccgg tatggcgttc ggaagaacga tctgccagta gattcccggt tcactcatcc
      361 cgtccacccg ggcggcgtca aggaactccg tcggaaaggt ggagaggtac tggcgcatct
      421 ggaagattcc gaaggcactg acgaggaagg gaagaatcaa gcccacataa gtgttttgga
      481 ttctgagttt catcaccacc atgtagagcg ggatcatgat cgcttcgaag ggaatcatca
      541 tcgtcgccat gatcatgatg aagatgacgt ttcggccccg gaaccggaat ttggccagtc
      601 catagccggt aaggctgcca agcaggacgg tggtgatcgc gacggtgacg gagacgatca
      661 gggagtttac gatgttccgg aggaagatgt agttttcatc cagaccgacg atcgcccgct
      721 tgaagttgat ccagttaagc gattcgggaa tccagttaaa cggcattcga agaatctggt
      781 tctttgtcat catcgaggcg gtgaccatga agacgatcgg ggtcagggta aagaagacca
      841 tcaccaagag gagtgcgtag atgagcaggt tgatggggtt gaatgttcgc cgtatctgca
      901 tcgtgccctc ctagtaatcg atattctcac cgctcttggt ggttcggaat tggaagagcg
      961 tcaagacgag catcagcacg aagaggacga cgctcatcgc gcttgccctt cccaggtact
     1021 gatcctggat tccggtgatg tagatgttga aggtgatgac gttgatcgga gcccgggtcg
     1081 agccgttggt atagaacatg tactgggtgc tgaacgtctt cagacactgc agcatcgcca
     1141 tgatggaaac aagcaccacg gtcggcttca aaagcgggag ggtgatccgc cagaacgtct
     1201 ggctcttgtt cgcgccgtcg atcgtcgccg cttcatagat cgtctgggga atcgtcgcaa
     1261 gaccggtgat gaagaggatg acgaaatagc
//
LOCUS       DAOPUZ010000403         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_122007_length_1288_cov_3.437956, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000403 DAOPUZ010000000
VERSION     DAOPUZ010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122007_length_1288_cov_3.437956"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKN79_06405"
     CDS             complement(<1..257)
                     /locus_tag="PKN79_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ95429.1"
                     /translation="MNAQLIERFIQKAFKYPQEDQEKILAAATFADSKHDGQKRASGE
                     PYIIHPLAVGEILIQLKMDADTISAGVLHDTLEDTDTTYEEL"
     gene            complement(254..1087)
                     /gene="prmC"
                     /locus_tag="PKN79_06410"
     CDS             complement(254..1087)
                     /gene="prmC"
                     /locus_tag="PKN79_06410"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNZ95430.1"
                     /translation="MTLNAWIAETKNLFIEQGVSDTPGLDARLLLGHATGYDQGRQIV
                     NAGEELASHRLDLLENLRSQRLAGRPIAYIIGSKEFYGRSFYVDERVLIPRPDTEILV
                     ETVLKAIEDGVVSSEDPIIDVGTGSGAVGITLAAELDCMVLLSDISCDALDVARINSR
                     RILGRELETIASDLLPLGRKYGIIVSNPPYLTDEWVAAVDQNVRSEPDLALRGFGEDG
                     LDIIRALVAESSNHGSLFIECDYRQTKQVAGLFEEHGFTNISIQRDLQGLERVVWGVR
                     T"
     gene            complement(1089..>1288)
                     /locus_tag="PKN79_06415"
     CDS             complement(1089..>1288)
                     /locus_tag="PKN79_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNZ95431.1"
                     /translation="EDRKSQVGSGDRSERIRTYNFPQNRLTDHRINLTLYKLDLIMAG
                     NLDEVIEPLKIASGEAALAEA"
BASE COUNT          263 a          361 c          358 g          306 t
ORIGIN      
        1 agttcctcgt aggtggtgtc ggtgtcttca agggtatcgt ggaggactcc ggcagagatc
       61 gtgtcggcgt ccatcttcaa ttggatgagg atttcaccga ccgccagggg gtggatgata
      121 tacggctctc cactggcgcg tttctggccg tcatgcttgg agtcggcgaa cgtcgccgca
      181 gccaggatct tctcctgatc ttcctgcgga tatttgaacg ctttttgaat aaaccgctca
      241 atcaactggg cattcacgtt cgtactcccc aaacaacacg ttccaacccc tgaaggtcgc
      301 gttgaatcga tatattcgta aagccatgct cttcaaagag cccggctacc tgtttcgtct
      361 ggcgatagtc gcattcgata aagagcgaac catggttcga gctctcagcg accagcgccc
      421 tgatgatatc gagtccgtct tctccgaacc ctcgcagggc gagatccggt tcagatcgga
      481 cgttttgatc aaccgctgca acccactcat cggtgaggta tggggggttg ctcacgatga
      541 taccatattt tcgcccgaga ggaagcaggt cgcttgcaat cgtctcaagc tcccttccca
      601 agattctccg gctgttgatc cgggcgacat ccaaggcatc gcagctgata tcgcttaaga
      661 gtaccataca atcgagttcg gcggcgaggg tgatgcccac cgctccgctg ccggtgccca
      721 cgtcgatgat cggatcttca gaggagacga cgccatcctc aattgccttg aggacggtct
      781 caacgagaat ttccgtatcc ggacggggaa tgagcactcg ctcatccacg tagaacgagc
      841 gcccgtagaa ctctttggaa ccgatgatat aggcgatcgg gcgtcctgca agccgttgag
      901 atctcaggtt ttcaaggaga tcgagccggt gggaggcaag ctcctccccg gcattgacga
      961 tctgtcttcc ctgatcatat ccggtcgcgt gtccaagaag gagacgggca tcaagaccgg
     1021 gggtgtcgct gactccctgt tcaatgaaga ggtttttcgt ttcagcaatc cacgcgttga
     1081 gggtcatcct atgcctcggc aagtgcagcc tctccgctgg cgatcttcag cggctcgatc
     1141 acttcatcca gattgccggc cataatcaaa tcaagcttgt acagggtaag gttgatccgg
     1201 tgatcggtga ggcggttctg gggaaagttg taggtgcgga tccgctcgct tcgatccccc
     1261 gagcccactt ggctcttgcg gtcctccg
//
LOCUS       DAOPUZ010000404         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_122698_length_1283_cov_3.609935, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000404 DAOPUZ010000000
VERSION     DAOPUZ010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122698_length_1283_cov_3.609935"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1283
                     /gene="nagZ"
                     /locus_tag="PKN79_06420"
     CDS             <1..>1283
                     /gene="nagZ"
                     /locus_tag="PKN79_06420"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNZ95432.1"
                     /translation="LMIGQHLVCGLERDEISEEFCRAVSHHKIANVILFARNIESKEQ
                     LMKLTADIQKLVRSECHTDALICVDQEGGMVTRLSPDFTNIPGAMALSATGKSRNAYD
                     AGFLTATELRSCGVNVDFAPTLDVNSNPDNPVIGVRSFSDDPSTVSEFGVAMARGLQE
                     GGVMACGKHFPGHGDTHIDSHLSLPTVTGDISAHLQPFKAAINNKIEGIMTSHILFPE
                     LEDRNLPATMSRTILTDLLKKEMGFEGLVFSDCMEMKAIADNWGTVDGALMALRAGVD
                     IVCISHHVNLGVEVVELMEKAIQDGTFGMTEFMELKKSTQKILRQKKRLAEVPVKPSS
                     IIGTKEHTRLNRELHEQSITLVNDVPFTLGANPLFIGPRPFRATNASDDGKQRHFGAA
                     LADLMGGDHLLVGDNPTEDEIGEVVKQAKNYESVV"
BASE COUNT          305 a          332 c          385 g          261 t
ORIGIN      
        1 gactgatgat cggacaacat ctcgtatgcg ggcttgagcg tgacgagata agcgaagagt
       61 tctgcagggc ggtttcacat cacaagatcg ccaatgtcat cctcttcgcg cgaaacatcg
      121 aaagcaagga acagctgatg aagctgacgg ccgatattca aaagctggtt cgctcggaat
      181 gccataccga tgcgctgatc tgcgtggacc aggagggggg catggttacc cgtctttccc
      241 cggattttac caacatcccc ggggcgatgg ctctttcagc gacgggcaag agcaggaatg
      301 cctatgatgc cggattcctc accgcaaccg agctccgctc atgtggggta aacgtcgatt
      361 ttgctcccac cttggatgtg aactccaacc cggacaatcc ggtgatcggc gtgcgcagct
      421 tctcggatga cccttcgacg gtgagcgaat tcggcgttgc gatggctcgg ggacttcagg
      481 aagggggcgt gatggcgtgt gggaaacact tccccggaca cggtgatacc catatcgaca
      541 gccacctcag cctaccgacc gtcacgggag acatttccgc tcatctccag ccgttcaaag
      601 cggcgataaa caataagatt gagggaatca tgacgagcca catcctcttt ccggagcttg
      661 aggatcggaa tcttccggcg acgatgagcc gaacgatcct caccgatctt ctgaaaaagg
      721 agatgggttt tgaaggcttg gtgttcagcg actgcatgga gatgaaagcg atcgccgaca
      781 actgggggac ggtcgacgga gcgttgatgg cccttcgagc cggggtggat atcgtctgca
      841 tcagccacca tgtcaatctg ggtgtcgaag tggtcgaact gatggaaaag gcgatccagg
      901 atggaacctt tggtatgacc gagttcatgg agctcaagaa gagtactcaa aagatcctca
      961 ggcaaaaaaa gagattggcg gaggttccgg tcaagccctc ttcgatcatc ggtacgaagg
     1021 agcatacgcg cctgaatcgg gagcttcacg aacagtcgat cacgttggtg aacgatgttc
     1081 ccttcacgct gggagcgaac cccctgttca tcggacctcg gccgttccgg gcgacgaatg
     1141 cttccgatga cggaaaacag cgacacttcg gggccgctct tgcggatctg atgggtggcg
     1201 accaccttct tgtcggggac aatccgaccg aagatgaaat cggcgaggtc gtcaagcagg
     1261 caaagaacta tgagagtgtg gtg
//
LOCUS       DAOPUZ010000405         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_124455_length_1272_cov_3.619556, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000405 DAOPUZ010000000
VERSION     DAOPUZ010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124455_length_1272_cov_3.619556"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1272)
                     /locus_tag="PKN79_06425"
     CDS             complement(<1..>1272)
                     /locus_tag="PKN79_06425"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNZ95433.1"
                     /translation="KHLRSFLGQIVNATFTLQGECAGAQAWSSFDTYCAPFVRSDNLE
                     YKEVKQAIQEFVFNLNVPTRVGFQCPFSNLTFDITPPKTLSEQPVIIGGALQDTCYGE
                     YQEEMDLINKAFCEVMEEGDASSRVFTFPIPTYNVTKEFPWESEVVDAIFSMTAKYGI
                     PYFSNYVSSDLSPEDALSMCCRLRLDTSELRKRGGGLFGSNPLTGSIGVVTINLSRLA
                     YESADENEFREKLLSAALLAKESLEIKRKIIEGETNRGMYPFSKVYLSSVKESQGTYW
                     ANHFSTIGVLGMEEACINLLGSSLLEDAGQAFGLRTLAYLREVIQSFQETSGGVLYNL
                     EATPAEGASYRLANLDRKAYPEIVTAGEKEHYYTNSSQLPVSSTKDIFEVLEKQDELQ
                     CQYTGGTVLHLYLDQRIKEAALAKLLVKRVFS"
BASE COUNT          250 a          355 c          370 g          297 t
ORIGIN      
        1 ggagaacacc cgtttgacca gaagttttgc caaggcggcc tccttgatcc gctggtcgag
       61 atagaggtgg aggaccgttc ctccggtgta ctgacactgc agctcatcct gtttctccag
      121 cacttcaaag atgtctttgg tggaagagac gggtagctgg ctggagttgg tgtaataatg
      181 ttccttctcc cccgctgtga cgatctcggg atacgctttt ctgtcgaggt tcgccagacg
      241 gtaactcgcc ccttcggcgg gggtggcttc cagattgtac aggactccgc cgctcgtctc
      301 ctgaaagctt tgaataactt cccgaagata ggcaagggtt ctcagtccga acgcctgccc
      361 tgcatcctcg agcaggctgc ttcccagaag gttgatgcac gcctcctcca tgccgagcac
      421 cccaatcgtg gagaagtggt tcgcccagta ggtcccttgg ctctccttca cgctcgaaag
      481 gtacactttg ctgaagggat acatcccccg attggtttcc ccttcgatga tcttgcgctt
      541 gatttcaagg ctctctttgg ccaacagggc ggcagacaac agcttttccc tgaactcatt
      601 ttcatcggcg ctttcatagg cgagccgact gaggttgatt gtcaccactc cgatcgagcc
      661 ggttaagggg ttcgaaccga agagcccgcc tccgcgcttg cgcaattcgc ttgtatcgag
      721 gcgaagccgg cagcacatcg acagagcgtc ctcggggctg aggtcgctgc ttacgtagtt
      781 ggagaagtag ggaatcccgt atttggcggt catcgagaag atcgcgtcga cgacctcgct
      841 ttcccacgga aactccttgg ttacgttgta ggtgggaatc gggaaggtga acacccgtga
      901 agaggcgtct ccctcctcca tcacttcgca aaacgccttg ttgatcagat ccatctcctc
      961 ctgatactcg ccgtagcagg tgtcttggag cgcccctccg atgatcaccg gctgctcgct
     1021 gagggttttc ggcggggtga tgtcgaaggt gaggttggaa aagggacatt ggaagcccac
     1081 ccgggtcgga acgttgaggt tgaagacgaa ctcctgaatc gcctgcttca cctccttgta
     1141 ctccaggttg tcggagcgga cgaagggagc gcagtaggta tcgaaggagg accacgcctg
     1201 agctccggcg cactcccctt ggagggtgaa cgtcgcgttg acgatctggc ctaaaaagga
     1261 acggaggtgc tt
//
LOCUS       DAOPUZ010000406         1260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_126358_length_1260_cov_3.392531, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000406 DAOPUZ010000000
VERSION     DAOPUZ010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126358_length_1260_cov_3.392531"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /gene="rplQ"
                     /locus_tag="PKN79_06430"
     CDS             complement(<1..343)
                     /gene="rplQ"
                     /locus_tag="PKN79_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNZ95434.1"
                     /translation="MKHRIGFNALSRNAAHRKAMKRNMVTSLFRYERIETTKAKALEV
                     RRMAEKMITRAKNDSVHNRRLIARDISDKAVLNKLFTEIAPLFTERNGGYTRILKTGN
                     RLGDAAEMVILE"
     gene            complement(333..>1260)
                     /locus_tag="PKN79_06435"
     CDS             complement(333..>1260)
                     /locus_tag="PKN79_06435"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNZ95435.1"
                     /translation="IGNTLRRVLLSSIQGYAVTAIKFTSFNEDGVPHLISSEYEQLPG
                     VREDISDIIAALKKLQIQMPEDAEGTNLLIECKGPGVVTGAALERDQVEIINKNLVLF
                     TMMDDANLEMEVQIDLGRGYVPSEINEKYIEEIGTIPLDATFSPVTRVKYSIEPTRVG
                     YRSDYDKLIIEVYTDGTIVPQNALAEAAKIAKDYFQIFINFDESLISSNDEVDEEEER
                     IRKILNTSVEELELTVRSSNCLKNANIRTIGDLTKKSEDEIAKTRNFGKKSLQEIKEK
                     LKEWNLSLGMTDYSILKTAIKVPEHKEEENEA"
BASE COUNT          243 a          343 c          325 g          349 t
ORIGIN      
        1 gctcaagaat gaccatctcg gcagcatccc cgagacggtt gcccgtcttc agaattctgg
       61 tatagcctcc gttgcgctcg gtaaagagcg gggcgatctc tgtgaaaagc ttgttgagaa
      121 cggctttgtc ggagatgtct cgggcgatca agcggcggtt gtgcacgctg tcgttctttg
      181 cccgggttat catcttctcc gccatcctcc gaacttccag agccttggct ttggtggttt
      241 caatccgttc atatcgaaac agactggtca ccatgttgcg cttcatcgcc ttgcgatgtg
      301 cggcgtttct gctcagcgcg ttgaacccaa tcctatgctt cattctcttc ttccttgtgt
      361 tcaggtactt tgatagcagt cttgaggatg ctgtagtcgg tcatgccgag gctgaggttc
      421 cactccttga gcttctcctt gatctcctga agggatttct ttccgaaatt cctcgtcttg
      481 gcgatctcat cctcgctctt cttcgtcaga tccccgatcg tgcggatgtt cgcgttcttg
      541 aggcagttgc tcgagcgaac ggtcaattcc agctcctcga cggaggtgtt gaggatcttt
      601 cggattcgtt cctcttcttc atcgacttca tcgttgctgg agatcagcga ctcatcgaag
      661 ttgatgaaaa tctggaagta atccttggcg atcttcgccg cttccgccag agcgttctgc
      721 ggaacgatcg tgccgtcggt gtacacctcg atgatcagct tgtcgtagtc ggagcgatat
      781 ccgacgcggg tcggctcgat cgaatacttc acccgggtca cagggctgaa tgtggcatca
      841 agcggaatcg tcccgatctc ttcgatgtac ttttcgttga tctcactggg aacatagccc
      901 cggccgaggt cgatctggac ctccatctca aggttcgcat catccatcat cgtaaagagc
      961 accagattct tgttgatgat ttcaacctga tcacgctcaa gggctgctcc ggtcacaaca
     1021 cccgggccct tgcactcgat gaggaggttg gtgccttcgg catcctcagg catctgaatc
     1081 tggagcttct tcaatgcggc gatgatatca ctgatatcct cacgcacacc gggaagctgt
     1141 tcatactcac ttgagatgag atgggggaca ccatcctcat tgaagctggt aaatttgata
     1201 gcagtgacgg catacccttg gattgaagaa agaaggaccc ttcgcagcgt attgccgatc
//
LOCUS       DAOPUZ010000407         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_126524_length_1259_cov_3.292359, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000407 DAOPUZ010000000
VERSION     DAOPUZ010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126524_length_1259_cov_3.292359"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..>1259
                     /locus_tag="PKN79_06440"
     CDS             94..>1259
                     /locus_tag="PKN79_06440"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNZ95436.1"
                     /translation="MKISLAGQYSKASIVRSSDRAITHYALDSRRCVSGSLFFTLRGK
                     NSSGIDYLKEAESNGAVAVLVHTEDIQRALNETSCAVLASDDVFQALMNLATAYRALF
                     PHVRTIGITGSTGKSTTKEAVGKIASALGPTTMTSGNLNSEYGLALSIMNLNSETKYG
                     VFELGIDHPGEMERMTKMASPSIGVLTNIGNAHLESMGSREVIAREKAKIFHPGIEAG
                     FISRHSLYGHLIQTLSPVELIRYSTRDVSAIDLGLDGWQISYQGVSFRIKAVGKHLLE
                     DVAGAIRVGAYLGARPVDIAHALEDFEPLVGRSTVRTSRVTIINDTYNASLDSTRSII
                     RYLSRLSWSGRKNAVLGPMKELGAESKQAHLEIAKTLIQSTFTKHFLYGREMEEA"
BASE COUNT          301 a          336 c          349 g          273 t
ORIGIN      
        1 cacgaaggcg atcccggaag tggtaagcgc ctactcttgg caggaagaga tcttcttcac
       61 gccgattacg gccgttgtcg cccgaggtga tctatgaaga tatcattggc gggtcaatac
      121 agcaaggcat caatcgtccg ttcatccgat cgggcgataa cccactatgc ccttgattcc
      181 cgccgttgtg tttccggctc cctgtttttc accctgcgag ggaagaactc gagcgggatc
      241 gattacctca aagaagcgga gagcaacggc gctgtcgccg ttctcgtcca taccgaagat
      301 attcaacgag cgctcaatga aacctcctgt gccgtgttgg caagtgatga tgtgtttcag
      361 gcactgatga atcttgcaac cgcctatcga gcactctttc ctcatgtccg gacaattggt
      421 attaccggtt caaccggcaa gagcacgaca aaggaggcgg taggaaagat cgcctccgct
      481 cttgggccaa ccacgatgac aagcggtaac ctcaacagtg aatacggttt ggctttgtcg
      541 atcatgaacc tgaactccga aacgaagtac ggggtgtttg agctgggcat cgaccatccc
      601 ggcgaaatgg agcggatgac gaagatggca agcccctcga tcggggtgtt gaccaatatc
      661 ggcaacgccc atttggaatc gatgggatcg cgggaggtga tcgcccggga aaaggcgaag
      721 atcttccacc ccgggattga agcggggttc atcagccgcc acagcctgta tggtcatctg
      781 attcaaaccc tcagtcccgt tgagttgatt cgatattcaa ctcgggacgt gagcgccatc
      841 gacctcggcc ttgacggatg gcagatttcc taccaaggcg tatcgtttcg aattaaagcg
      901 gtcgggaaac acctccttga agatgttgca ggtgcgatcc gagtcggcgc ttacctcggt
      961 gcccgaccgg tggatatcgc ccatgcgctg gaggatttcg aaccgctggt cggccgatca
     1021 accgtccgga catcccgcgt gacgatcatc aacgatacgt acaacgccag tttggattca
     1081 accagatcga tcatccggta tctttcccgc ctttcctgga gcggacggaa gaatgcggtt
     1141 ctcggaccga tgaaagagct gggggcggag agcaaacaag cccatcttga aattgcaaaa
     1201 acattgattc agtcaacatt tacgaaacac tttctctacg gacgggagat ggaggaagc
//
LOCUS       DAOPUZ010000408         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_126697_length_1258_cov_3.250208, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000408 DAOPUZ010000000
VERSION     DAOPUZ010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126697_length_1258_cov_3.250208"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..>1258
                     /locus_tag="PKN79_06445"
     CDS             104..>1258
                     /locus_tag="PKN79_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95437.1"
                     /translation="MSFVFLLGTNYYNYQLVGMMVGVPHVGLLMGAAFATLSLLIFAF
                     PAAINILHAAKEIERIRALAISESELALSRMIIFYFNFFPIYLFFVIPALIVGIMTTG
                     FSFFYLLSSLLLLLVGPMIPISLVSLLEVGVVHLTKGRRAQRSGELFYLVVMMALVIG
                     ISSQMGKNAEMTGNLEELTHQLAPVIKKLTRFLAPFALQAQGLYNPILLLVWLAGALL
                     LAYITFTVTAGTYHHACSLLASGGYRTKKRRKNNTGEQKPIVALMKRDWTILLSTSGF
                     IFELGGELLIPLILIITYTAMGIIGDISQATKVLAQYPIFEPVVVLIVLLMSNINLLS
                     CTSVSRQGKLALHDRLYPLEPKIFVNAKFYLHMALVGTTNTIYLLAATLIL"
BASE COUNT          252 a          361 c          304 g          341 t
ORIGIN      
        1 caagccgctt cgatcggtga cccagcgctt cagcttcgga aagaagaaat cggtacggac
       61 gatcagcggg gtggttcttt cacttttgat gctcgcctcg ctcatgagct ttgtattttt
      121 gcttggaacg aactactaca actatcaact ggtcgggatg atggtcggcg ttccccatgt
      181 cggtcttctg atgggcgcgg cattcgcaac gttgagtctg ctcatcttcg cgtttcccgc
      241 agcgatcaac atcttgcacg cggcgaaaga gatcgagcgg atcagggcat tggcgatcag
      301 cgaatcggag cttgccctca gccggatgat catcttctat ttcaattttt tcccgatcta
      361 tctcttcttc gtcatccctg ctctcatcgt cggaatcatg accacgggct tttccttctt
      421 ctacctctta agctcgctgc ttttgcttct tgtcggaccg atgattccga tttcgctggt
      481 ttcgctgctc gaggtcggtg ttgtccatct gaccaagggg aggcgggctc aacggtcggg
      541 cgagctcttc tacctggtcg tcatgatggc cttggtgatc ggcatctcct cccagatggg
      601 gaaaaacgcc gagatgaccg gcaatctgga agagctgacg catcagcttg ccccggtgat
      661 caaaaagctg acccgatttc ttgctccgtt tgccttgcag gctcaaggtc tgtacaatcc
      721 gatcctgctc cttgtgtggc tcgcaggtgc gctccttctt gcctacatca cctttactgt
      781 aaccgccgga acctatcatc acgcctgctc cctgctggct tccggcggat accggacaaa
      841 aaagcgaaga aaaaacaata caggcgaaca aaaaccgata gtcgcgttga tgaagcggga
      901 ctggacgatc cttctctcga ccagcgggtt tatctttgaa ctgggcgggg aattgctgat
      961 tcccctgatt ctcatcatca cctacacggc aatgggaatc atcggcgaca tctctcaggc
     1021 gacgaaggtt ctcgcccaat atccgatctt tgaaccggtt gtcgttctca tcgtgctgct
     1081 catgtccaat ataaacctgc tttcctgcac cagtgtatcg cgccaaggca agcttgccct
     1141 ccatgatcga ctgtatcccc ttgagcccaa gatcttcgtc aatgcgaagt tttatcttca
     1201 catggctctt gtcggtacga ccaatacaat ctatcttctt gccgctacct tgatcctc
//
LOCUS       DAOPUZ010000409         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_127959_length_1250_cov_3.097071, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000409 DAOPUZ010000000
VERSION     DAOPUZ010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127959_length_1250_cov_3.097071"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKN79_06450"
     CDS             <1..318
                     /locus_tag="PKN79_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95438.1"
                     /translation="VVDGNLYTAVPFFFKYSYLPVQGTIDLPISVGLGFVYNSGGANT
                     SIMTLYSNIELGLTYFPGEHWGFGVRTGLWLIPELNYRKELFGDNALAGFVPITLSVS
                     YRQ"
     gene            328..>1250
                     /locus_tag="PKN79_06455"
     CDS             328..>1250
                     /locus_tag="PKN79_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95439.1"
                     /translation="MKKILIIMTTITLLFTACNADATSGLFRTAAEAREALTIKYMQV
                     VGRKGANDTLYFLTREGLFVKDTNAKAAELIVTSKEDNRVQSAAYLNDKIKLLYKTDK
                     NDGSLFEYDPVTKASTPLPIPAAPVTDFRLMPNGKIVLKGAANSGEFWIVANDKLGSY
                     NADGIKVTAGEGFDFYGLFTPSNYEKSDANKGVLFSFVKSGSPNEYKNYYYDGAGVNE
                     MNNTLGSISNFSVIGTDLYILTVEGALYHLKTGALNVAAKLKDNTVGYERNAFFYPID
                     DGANLHFITNPSSSDSMSVFSVVKGDPKADTT"
BASE COUNT          327 a          325 c          306 g          292 t
ORIGIN      
        1 gtggtggacg gcaatctcta taccgcggta ccgttcttct tcaagtacag ctaccttccc
       61 gttcagggaa cgatcgatct tcccatcagt gtcggtcttg gcttcgtgta caactccggc
      121 ggggccaaca cctcgatcat gaccctgtat tcaaacatcg aactgggatt gacctacttc
      181 cccggcgaac actggggatt cggcgtccgg acaggattgt ggctcattcc cgaactcaac
      241 tatcgaaagg agctcttcgg tgacaacgcc ctcgccggct tcgttcccat caccctctcg
      301 gtaagctata gacagtagga ggaaacaatg aaaaaaattc ttatcatcat gacgacaata
      361 accctcctct tcaccgcctg caacgccgat gcgacgagcg ggctcttcag gacggcagcg
      421 gaagcccggg aagcgctgac gatcaagtac atgcaggtcg tcggaagaaa aggtgcgaac
      481 gatacgctgt acttcctcac cagggaaggg ctttttgtca aagatacaaa tgcaaaagca
      541 gctgaattga tcgttacaag caaagaagac aaccgggttc aaagcgccgc ctacctgaat
      601 gataaaatca agcttctcta taaaacggat aaaaatgatg ggtcgttgtt cgagtatgat
      661 ccggtaacca aggcatccac tccgcttccg attcccgccg ctccggtcac cgacttcaga
      721 ctcatgccca acgggaagat cgtgctgaag ggggcggcga attcaggtga attctggatc
      781 gtagcaaatg acaaattagg tagctataac gctgacggta taaaggttac agcaggcgag
      841 ggttttgatt tctatggtct cttcactcct tcaaactacg aaaagtcgga tgcgaacaaa
      901 ggggttctgt tctcgttcgt gaaatccggt tcgcccaatg aatacaagaa ctactactat
      961 gatggagcgg gtgtgaatga aatgaacaac acccttggtt ccatctccaa tttctccgtc
     1021 atcggaacag atctgtatat cctgaccgtt gaaggggctt tgtatcattt gaagacgggc
     1081 gccttgaatg ttgctgccaa gcttaaagac aatactgtcg ggtatgagcg caatgccttc
     1141 ttctacccca ttgatgacgg agcgaatctc cacttcatca ccaacccctc ctcttccgat
     1201 tcgatgagcg tcttcagtgt ggtcaagggg gatccgaaag cggatacgac
//
LOCUS       DAOPUZ010000410         1245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_128770_length_1245_cov_3.179832, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000410 DAOPUZ010000000
VERSION     DAOPUZ010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128770_length_1245_cov_3.179832"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="PKN79_06460"
     CDS             <1..1032
                     /locus_tag="PKN79_06460"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ95440.1"
                     /translation="LSRQIIGSLLRRKADEGFLTIDGVRHFLAATLLHDIGHFPFTHS
                     LKELPLKSHEQLATELLENDRILRTALKDAALDTKIIGDIIDEERPTNNEEVLLYRSI
                     LSGALDPDKLDYLNRDAYFAGVPYGVQDVSYITDHLYLHEGALAIGFEALGSIEHLLF
                     SKYLMYRNVYRHTSVRSATAMIKRCLYENLKRGTLDEDGLYGLDDESFFLLSQTERPL
                     GNLLTRVRENRLFQPVVEELFDEKNPIHTNALSLRQRSEYEERIEKDLNLIGGSIVID
                     IPERVSFESDIPVVNHQTFLGRFEDVDPLFTRSRAMEFTSSLRTVRLFADPSIDPTVL
                     RHAWEHIHE"
     gene            1025..>1245
                     /locus_tag="PKN79_06465"
     CDS             1025..>1245
                     /locus_tag="PKN79_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine nucleotide alpha hydrolase"
                     /protein_id="HNZ95441.1"
                     /translation="MSEKLPYQTLLEGDLPPWVMRLVKATGRAINRYSMIRDGDTILL
                     GTSGGKDSLALALALSLRKKWLPIDYTLKA"
BASE COUNT          292 a          355 c          325 g          273 t
ORIGIN      
        1 ctctctcggc agatcatcgg cagtctgctg aggcgaaagg cggatgaagg gtttctcacg
       61 atcgacggtg tccggcattt ccttgccgcc accctcctcc atgatatcgg acacttcccg
      121 ttcactcaca gcctcaaaga actgccgctc aaatcgcacg aacaattggc gacggaactg
      181 cttgaaaacg accgaatcct ccgtactgca ttgaaagatg ccgctctgga tacaaaaatc
      241 atcggtgaca tcatcgatga agagcggccg acgaacaacg aagaagtgct cctgtatcgc
      301 tcgatcttaa gcggtgcgct tgatcccgac aagctcgact acctcaaccg ggatgcgtac
      361 tttgccggcg ttccctatgg agttcaggac gtttcctaca ttaccgatca cctctacctc
      421 cacgaaggag cgcttgcgat cggctttgag gcgctcggat cgattgaaca tcttctgttt
      481 tcaaagtacc tgatgtaccg gaacgtgtac cggcacacat ccgttcggag cgcaacggcg
      541 atgatcaagc gctgcctcta tgaaaacctc aaacggggga cccttgacga agacgggctc
      601 tatggccttg atgacgagag cttcttcctg ctcagccaaa ccgaaaggcc attggggaat
      661 ctcctgaccc gggttcggga aaacaggctt ttccaaccgg tggtcgaaga gctcttcgat
      721 gaaaagaatc cgattcacac caatgccctc agcctcaggc aacgcagtga gtatgaagaa
      781 cggattgaaa aagacctgaa cctcataggc ggatcgatcg tcatcgatat cccggaacgg
      841 gtttcctttg aatccgatat cccggttgtc aatcatcaaa cgttcctggg gaggttcgaa
      901 gatgtcgatc ccctcttcac ccgaagccgg gcaatggagt tcacctcatc gctgaggacc
      961 gttcgcctct ttgccgatcc ttccatcgat ccgacggtgc tccgccatgc ttgggagcat
     1021 atccatgagt gagaaactcc cctatcaaac gcttcttgag ggagatcttc ccccgtgggt
     1081 gatgcgtctg gtgaaagcga cggggagggc gatcaaccgg tactcgatga tcagggatgg
     1141 agatacgatt ctgcttggta ccagcggcgg aaaggacagc cttgccctcg ccttggcgct
     1201 ttcgctgcgg aagaagtggc tgccgatcga ctataccctc aaggc
//
LOCUS       DAOPUZ010000411         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_131584_length_1228_cov_3.138107, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000411 DAOPUZ010000000
VERSION     DAOPUZ010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131584_length_1228_cov_3.138107"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1135
                     /locus_tag="PKN79_06470"
     CDS             71..1135
                     /locus_tag="PKN79_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95442.1"
                     /translation="MTYYPLRERKNKVYIEDATILPENDSFPLADEQEVWIDALCDEI
                     LEARRNKHPVILAFGAHTIKNGLGQLLGRFAKEGYFTHLATNGAGIIHDWEFAYQGRS
                     SEDVKANVAEGKFGTWEETGFSLNLALAVGAFEGCGYGESIGKMIEEDGVMIPTQEEL
                     LETSLDQSASFEKRSGALDLLDLIVKLDLQPGWLGIDHPYKEYSLQHRSYVSDVRFTG
                     HPMFGHDIIYTHKANRGSVIGRTAERDFLVFVDSIARLEGGVYLSVGSAVMSPMIFEK
                     ALSMVRNTGVRIDHAVIRVVDLQKGTWDWNRGEPPEDNPAYYQRFMKTFSRMGLKSHY
                     LCIDNRSLFVNLYTALKRKG"
BASE COUNT          292 a          311 c          358 g          267 t
ORIGIN      
        1 gcttcctcga aggagctgat gaatcttttg gggtggaatg gcagtgaaca gtatgtttga
       61 tcgtactcga atgacctatt atcctcttcg cgaacgaaag aacaaggtat acatcgaaga
      121 tgcaaccata cttccggaga atgattcctt tccgcttgcc gatgaacagg aggtctggat
      181 cgacgccctg tgtgacgaga ttcttgaagc acggcgaaac aagcatccgg tgattctggc
      241 gttcggtgcc catacgatca agaacgggct gggacaattg ctcggccgct ttgcaaagga
      301 ggggtatttc acccatctgg cgaccaacgg agcgggcatc atccatgact gggagttcgc
      361 ctatcagggc cgatccagcg aggatgtcaa agcgaacgtg gccgaaggga agttcggcac
      421 gtgggaggag acgggctttt ccctgaatct tgcgctggcg gtcggagctt tcgaaggctg
      481 cggctacggc gaatcgatcg ggaagatgat cgaagaggat ggggtgatga tccccacaca
      541 agaagagctc ctggaaacct ccctcgatca aagcgcatcc ttcgagaaac gaagcggtgc
      601 cttggacctg ctcgatctga tcgtcaagct ggatcttcag ccgggttggc tgggcatcga
      661 ccacccatac aaggaatatt cgcttcagca ccgctcctat gtaagcgatg tccgcttcac
      721 cggccacccg atgttcggtc acgacatcat ctacacccac aaggcgaaca ggggctcggt
      781 gatcggacgg accgccgagc gggattttct tgtcttcgtc gattcgatcg cccggctcga
      841 agggggtgtc tatctctccg tcggatcggc ggtgatgagt ccgatgatct ttgaaaaagc
      901 gctttccatg gttcgcaaca ccggcgttcg gatcgatcat gcggtcatca gggtggtgga
      961 tcttcaaaaa ggaacctggg attggaaccg gggcgagccg cctgaagaca acccggcgta
     1021 ctaccagcgc ttcatgaaaa cattcagccg gatgggcctg aaaagccatt atctctgcat
     1081 cgacaaccgg tcactgtttg tgaatctcta taccgctctc aaaaggaaag gatgaatcga
     1141 tatgaagaag aagtttacag cgtggaagga tgaactgcag catcacctgg acaaggagct
     1201 gttgccgttc tggcttgacc gtctatgg
//
LOCUS       DAOPUZ010000412         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_131886_length_1226_cov_3.748079, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000412 DAOPUZ010000000
VERSION     DAOPUZ010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131886_length_1226_cov_3.748079"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PKN79_06475"
     CDS             complement(<1..1192)
                     /locus_tag="PKN79_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNZ95443.1"
                     /translation="MGAFKAYDIRGIYNVDFNKDTVYKIGYFLPKLLKSKAVLVGRDV
                     RTSSDEIFEALSRGIMDSGADVWTIGLATTPMVYFATVHFKADASVQITASHNPAEYN
                     GLKISRTNAVPVGSDSGLKELEAMVLNDEIVPSRKKGKIIKKEARNAYIDFLKAFTPD
                     TSDLNLSIDFSHGMANLIVKDLLGENHHYLYDHLDGTFPAHEPNPLEVENAYDLRDAV
                     IANKSDIGIIYDGDADRVMFLDENGRFLQPDYITSLLGLYYLKKEKGRVLVDIRTSRS
                     TVEYLESLGAEVHVWKVGHAFAKNKLRELGAIFGGELAGHYYFRDFFNCDSGILASLL
                     VLQAVKMLKAEGQTIGSFIDSIVRWANSGEVNFTLSEKDGAMTALYERYVKNDSPTLV
                     MAFDG"
BASE COUNT          276 a          312 c          317 g          321 t
ORIGIN      
        1 agccgtcgaa agccatgaca agggtggggg aatcgttttt gacatatcgc tcatacagag
       61 cggtcatcgc tccatccttc tcactgaggg tgaagttcac ctctccgctg tttgcccacc
      121 tgacgatcga atcgatgaag gagccgattg tctgaccttc agctttgagc atcttcacag
      181 cctgaaggac gaggagggaa gcaagaatgc cgctgtcgca gttgaagaag tctctgaaat
      241 agtagtgtcc ggcaagctcg ccgccgaaga tcgctccaag ctcccgaagc ttgttcttcg
      301 cgaacgcatg accgactttc cacacgtgaa cctcggcacc gaggctttca aggtattcaa
      361 ccgttgaacg gctggtacga atatcaacca gaactcggcc tttttccttt ttgaggtaat
      421 agaggcccaa gagactggtg atgtaatcgg gttggagaaa gcggccgttc tcatcgagga
      481 acatgacgcg gtcggcatcg ccgtcgtaga tgattccgat gtcgcttttg ttggcaatta
      541 ccgcatcgcg aagatcgtag gcgttttcga cttccagagg attgggctca tgagccggga
      601 atgttccgtc aagatgatcg tagaggtaat gatgattctc ccccaagaga tctttgacga
      661 tgagatttgc cattccatgt gaaaagtcga tcgaaaggtt gaggtcggag gtgtcgggag
      721 tgaacgcttt caaaaagtcg atatatgcgt tccttgcttc tttcttgatg atctttcctt
      781 tctttcggga gggaacaatt tcatcattaa gaaccatcgc ttccaattcc ttcagtccgc
      841 tgtccgaacc gacggggacg gcattcgtcc gactgatttt cagtccattg tactcggcgg
      901 ggttgtggct ggctgtgatc tggacggagg catccgcctt gaagtgaacg gtcgcaaaat
      961 acaccatcgg ggtggtcgcc agaccgatgg tccagacatc cgctccgctg tccatgattc
     1021 ccctgctcaa tgcttcaaag atctcatcgc tgctggttcg gacatcccga ccgacgagga
     1081 ccgctttgct cttcagaagc ttgggcaaaa agtagccgat cttgtacacg gtgtctttgt
     1141 tgaaatccac gttgtagatt ccccgaatat cgtatgcttt aaacgctccc atcgtttaat
     1201 ctccttctga actcctttcg atccat
//
LOCUS       DAOPUZ010000413         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_131956_length_1226_cov_2.278395, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000413 DAOPUZ010000000
VERSION     DAOPUZ010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131956_length_1226_cov_2.278395"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /gene="rsmH"
                     /locus_tag="PKN79_06480"
     CDS             complement(<1..909)
                     /gene="rsmH"
                     /locus_tag="PKN79_06480"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNZ95444.1"
                     /translation="MNYVHTSIMPNELLTHLVPQSSDPIMVDATCGEGGHTHLFLSTY
                     PNLKVIGVDRDREIQEKARVRMEEFGPRFKPVNMWFDVFFKEAEDSSFDLILFDLGIS
                     MFHFNESQRGFSLRKGEPLDMRLDSEDAISAFDVVNRYDEKKLANVIYAYGEERYSRR
                     IARAIVQHRKTKTIATTDDLANIIYKSVPPAYRYQKIHPATRTFQAIRIEVNHELDRI
                     EPALTEALRTLKVGGRLAVITFHSLEDRPVKWLFKNLAQEDRPRLNVLTKKPLVPGEE
                     EIARNPASRSSKLRVVEKIREEERHGA"
     gene            complement(909..>1226)
                     /locus_tag="PKN79_06485"
     CDS             complement(909..>1226)
                     /locus_tag="PKN79_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNZ95445.1"
                     /translation="EDFEQLKNTIINGPHSMFDRRLRILQRGMIAPAQLCEFDKAGRI
                     NIPPSLRESVKLLLKEESVLLGAGNYLELWNKEAYEAYLEESMDEFLDAAQSLSNLGR
                     EEE"
BASE COUNT          266 a          330 c          320 g          310 t
ORIGIN      
        1 tgctccatga cgctcctcct cacgtatttt ttccacgacc cgaagtttgg agctacggga
       61 cgccgggttt cgggcaatct cctcttcacc gggaacaagc ggctttttcg taagtacgtt
      121 cagcctcggc cgatcttcct gagcaagatt cttgaacaac cactttaccg gtcgatcttc
      181 caacgagtgg aacgtgataa ccgccagcct gcctccgact ttcagggttc tcagcgcctc
      241 ggtgagagcc ggttcgattc gatcaagttc atggttcact tcgattcgga tcgcttggaa
      301 ggtgcgggtc gccggatgaa tcttttgata gcgatacgcc ggagggacgc tcttgtagat
      361 gatgttcgcc agatcatccg tcgtggcgat ggtttttgtt tttcgatgct ggacgatcgc
      421 ccgggcgatt cttcgcgagt agcgctcttc cccgtaggcg tagattacgt tggcaagctt
      481 cttctcatcg taccggttca ccacatcgaa cgcggagatc gcatcttcac tgtcgagacg
      541 catatcgagg ggttcgcctt tgcgaagaga aaaccccctt tgcgattcgt tgaaatgaaa
      601 catggagatt cccaaatcga aaagaatcag atcgaaggag gaatcctccg cttccttgaa
      661 gaacacatcg aaccacatat tgaccggttt gaaacgaggg ccgaactcct ccatccgaac
      721 ccgtgctttt tcctggattt cccgatcccg atcgactccg atcaccttga ggttcggata
      781 ggtggaaaga aaaagatggg tgtgcccccc ttcgccacag gtggcatcaa ccataatcgg
      841 gtcggaactt tggggaacaa ggtgagtaag cagttcgttg ggcatgatcg aggtatggac
      901 gtagttcatc actcctcctc tctgcccagg ttcgacaggg actgagcggc atcaaggaac
      961 tcatccatcg attcctcaag gtatgcctcg tacgcctctt tgttccacag ctccagatag
     1021 ttgcccgctc ccagaagaac cgattcctct ttcaagagca gtttcacgct ttcacggagg
     1081 gaagggggaa tgtttatccg accggcttta tcaaattcac agagctgggc gggagcgatc
     1141 atcccccgtt gaaggattcg gagccttcgg tcgaacatcg agtgcgggcc gttgatgatg
     1201 gtgtttttca gctgctcaaa atcttc
//
LOCUS       DAOPUZ010000414         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_132786_length_1221_cov_3.227273, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000414 DAOPUZ010000000
VERSION     DAOPUZ010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132786_length_1221_cov_3.227273"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..665)
                     /locus_tag="PKN79_06490"
     CDS             complement(63..665)
                     /locus_tag="PKN79_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNZ95446.1"
                     /translation="MINLGPHQKGATNRALAEVAKGLDEYKVEHEILWVGTDPIQGCI
                     DCKACFKNRRCVFDDLVNTAVEKVRASDGLILGSPVHYASAANTLFVDRLFRVVSKEV
                     ALKVGSSVVSCRRGGASATFDQLNKYFTISQMLVVSSAYWNSVHGNNASEVEQDHEGL
                     QVMRILGRNMGYVINRLHGRDVEKPLPEQRVWTNFISSER"
     gene            complement(679..>1221)
                     /locus_tag="PKN79_06495"
     CDS             complement(679..>1221)
                     /locus_tag="PKN79_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNZ95447.1"
                     /translation="GLQPANEKRKAKESRIYELSQVGEVPTEAGYQIPFRHLCNLLQL
                     HEGDIDRALSTLEDATPDQLERLKTRAVCAWNWITTFSPEDFRFRLMKDSDPKVEIEG
                     AELAAIHSLREVVKEMDTLDEQTYTTRLYDAAKDNGLDTGDFFKLVYRVLIGRSHGPK
                     LASFLETIGREKALEILSRY"
BASE COUNT          261 a          369 c          324 g          267 t
ORIGIN      
        1 gcgattgtca cgaacaacaa ccccgggatt ccacatcgac ctgtgactgg gcagcaactc
       61 cgtcatcgtt ccgaagaaat gaagttcgtc cacacccgct gttcaggaag aggtttttcc
      121 acgtctcttc catggagacg gttgattaca taccccatgt ttcgtccgag aatccgcatc
      181 acctgaagcc cctcatgatc ctgctccact tccgacgcat tgttcccgtg gacgctgttc
      241 caatatgccg aggagacgac cagcatctgg ctgatcgtga aatatttgtt caactgatcg
      301 aacgtggcgc tcgctccgcc ccggcggcac gagaccaccg aagagccgac tttcagggcg
      361 acctccttgg atacgacccg gaaaagacgg tcgacgaaga gggtgttggc ggccgaggcg
      421 taatgcaccg gtgaacctaa gatcaaacca tcggaagctc ggaccttttc caccgccgtg
      481 ttgaccagat cgtcaaacac gcagcggcgg ttcttgaaac aggctttgca atcgatgcac
      541 ccttgaatag gatcggttcc cacccaaaga atctcatgct cgaccttgta ttcatcaagc
      601 cctttggcga cctcggccag ggcgcggttg gtagcgccct tttggtgagg ccccaggttg
      661 atcatcaaca ccttcatatc agtacctgct gagaatctcc agcgctttct cccgaccgat
      721 cgtctcgagg aacgaagcga gcttcggtcc gtgggaacgg ccgatgagaa cccggtagac
      781 gagcttgaag aaatcgccgg tatcaagacc gttgtccttc gccgcatcat agaggcgggt
      841 ggtataggtc tgctcatcca gcgtatccat ttccttcacg acctcccgaa gactgtggat
      901 cgccgcaagt tcggcgccct cgatctccac tttgggatcg ctgtccttca tcaggcggaa
      961 ccggaaatcc tccgggctga acgtggtgat ccaattccaa gcgcagaccg cccgggtctt
     1021 caagcgttcc agctggtcgg gggtggcatc ctccaaggtg gaaagagccc gatcgatatc
     1081 cccctcatga agctgaagaa ggttgcacaa atgacggaac ggaatctggt agcctgcctc
     1141 ggtgggaacc tcccccactt ggctgagctc gtagatccgg ctttctttcg ccttgcgctt
     1201 ctcgtttgcc ggctgaaggc c
//
LOCUS       DAOPUZ010000415         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_133714_length_1216_cov_2.351421, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000415 DAOPUZ010000000
VERSION     DAOPUZ010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133714_length_1216_cov_2.351421"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKN79_06500"
     CDS             complement(<1..279)
                     /locus_tag="PKN79_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNZ95448.1"
                     /translation="MAEEKKKTQSEAQLQEKLLKTRSVLLSGEINKESADEVIRQLLV
                     LEGESNEPIRLYINSPGGDVDAGYAIFDMVRFINAPVTMIGMGLVASAA"
     gene            395..>1216
                     /gene="era"
                     /locus_tag="PKN79_06505"
     CDS             395..>1216
                     /gene="era"
                     /locus_tag="PKN79_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNZ95449.1"
                     /translation="MKSATVAVVGRPSAGKSTLVNTICEMKVSITAKTPQTTRNAIKG
                     IYTDQRGQLIFIDTPGYHLGTRVLNLRLQETAVKSLSESDLVLYLIDGKRSAKEEELS
                     LAQTLAKVKTPVICLINKADILTEEEIEEAKAFLKEHLPSSPILIGSARNDEGIDEVL
                     IELFKVAPEGPLLYDEDAYTDQELEFRIAELIREKAIALVTEELPHAIYVDVADIEYK
                     EETNTIWIRAFIVVERESQKGIVIGKGGENMTKIRKAVEPEIRSIFPKRKLRLDLK"
BASE COUNT          300 a          357 c          322 g          237 t
ORIGIN      
        1 cgcggcggac gccacaaggc ccatcccgat catcgtcacc ggagcgttga tgaaccgaac
       61 catgtcgaag atcgcatatc cggcatccac atcccctcca gggctgttga tgtagagcct
      121 gatcggctca ttgctctccc cttccagaac aaggagctgc ctgatcactt catcggcgct
      181 ttccttattg atttcaccgc ttaacagaac gctgcgggtc ttcagcagct tttcctgcag
      241 ttgtgcttca ctttgcgttt tctttttttc ttccgccatg acggcctcac tttcgaacga
      301 tagaatggat atatcaagta taggcaaatt gcaaggggat gaaaagcatc aaaacgagat
      361 tcccacacac cggcaaggaa gatatactgg agatatgaaa tctgcaacgg tagcggtcgt
      421 tggccgcccc tcggcgggaa aatcaacgct ggtgaacacg atctgcgaga tgaaggtctc
      481 gatcacggca aaaactcctc agaccacccg caatgcaatc aaggggatct acaccgatca
      541 gcggggccag ctgatcttca tcgacacccc cggctatcac cttggtaccc gggttctgaa
      601 cctccgcctt caggagaccg cggtcaaaag cctgagcgaa agcgacctgg tcctctatct
      661 cattgacgga aagcgcagcg ccaaggagga ggaactttcg ctcgcccaga cgctcgccaa
      721 ggtgaaaacc ccggtcatct gcctaatcaa caaggcggac atccttaccg aggaggagat
      781 cgaggaggcg aaagcgtttc tgaaggagca tttgccttct tccccgatcc tcatcggaag
      841 cgccagaaac gacgagggaa tcgacgaggt cctgatcgag cttttcaagg tcgcccccga
      901 aggtccgctt ctctacgacg aggatgcgta caccgaccag gagctggagt tccggatcgc
      961 cgagctgatc cgggaaaaag cgatcgccct cgtcaccgag gagcttcccc atgcgatcta
     1021 tgtggacgtg gcggacatcg aatacaagga agagaccaac acgatctgga tccgggcatt
     1081 catcgtcgtc gagcgggaat cccagaaggg catcgtgatc ggcaagggcg gagagaacat
     1141 gacgaagatc cgcaaggcgg tcgaaccgga gatccgttcc atcttcccca agcgcaagct
     1201 ccgcctcgac ctcaag
//
LOCUS       DAOPUZ010000416         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_133894_length_1215_cov_2.393966, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000416 DAOPUZ010000000
VERSION     DAOPUZ010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133894_length_1215_cov_2.393966"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKN79_06510"
     CDS             <1..478
                     /locus_tag="PKN79_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95450.1"
                     /translation="LGPFLLFMTSTAFHSLLLRLGFATKRFKAMTMVQIVQNVLDMLL
                     MVLLIKGNAGLQALSLANGISYLLSLFIILFIVKDLYTPFTDKVLFKEIGKIILANIP
                     ILIGAILYKLLAPPWYTDGSTWKNLGILIVIGLSSIFVWLTSYKRFKIDFFTILSR"
     gene            complement(475..>1215)
                     /locus_tag="PKN79_06515"
     CDS             complement(475..>1215)
                     /locus_tag="PKN79_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ95451.1"
                     /translation="EYLAIEEALAYPIIPLTEILFEHRRHTMAIVQDVNVFPHFNQYL
                     QFGYYPFYREGKADYKSRLAETIRYIIERELPFQRNVAPSAVPKIKLLLMIIAESVPF
                     TPNISKLSERIGISRQTLITYLHYLEEAKIISLLYKSTGGISLLQKPEKIYLDNTNLI
                     HLLGEKEPDVGSIRETFLASMLRSKTSIRYTDHGDFLVNNTHTIEAGGKNKTRKQIQT
                     VDDGFIAADMIEHGSDRRVPLWLFGFLY"
BASE COUNT          270 a          323 c          283 g          339 t
ORIGIN      
        1 gctggggccc ttcctgctct tcatgacgag caccgccttc cattccctgc ttctaagact
       61 cggtttcgca accaagcgct tcaaggcaat gacaatggtg cagatcgttc agaacgttct
      121 tgatatgctc ttgatggttc tcctcatcaa aggaaatgcg ggccttcaag ccctctcgct
      181 ggccaacgga atctcctacc ttctttccct gttcatcatc ctcttcatcg tgaaagacct
      241 ctatacaccg ttcaccgaca aggttctctt caaggagatc gggaagatca tcctggcaaa
      301 catcccgatc ctcatcggag cgattctcta caagctcctt gcaccgccct ggtacacaga
      361 cggctccact tggaagaacc tcggcatcct catcgtcatc gggctctcat ccatcttcgt
      421 atggctgaca tcatacaagc gattcaaaat cgatttcttc acgatccttt cccgctaata
      481 gagaaacccg aaaagccaaa gcggaactct tcggtcgctt ccgtgttcga tcatgtccgc
      541 tgcgatgaat ccgtcatcca cggtctgaat ctgtttcctc gtcttgttct ttccgcccgc
      601 ttcgatcgta tgcgtattgt tgacaaggaa atctccatgg tcggtgtagc ggatgctcgt
      661 tttgcttctc agcatggaag caaggaacgt ttctcgaatg cttccgacat ccggttcctt
      721 ttctccgaga agatggatca ggttcgtgtt gtcaaggtag atcttttccg gcttctgaag
      781 cagggagatt cctccggtcg acttgtagag gagggatatg atcttggctt cttccagata
      841 gtggagatag gtaatcaatg tctgccgact tattcctatc cgttcactca gtttggagat
      901 attcggagta aatggcaccg attcggcgat gatcatgagc agcagcttga ttttcggtac
      961 ggcggagggc gctacattcc gttgaaacgg aagttcgcgt tcgatgatgt accgaatcgt
     1021 ctcggccaat ctgcttttgt aatcggcttt tccctctcga taaaaagggt agtagccaaa
     1081 ctgcaagtat tgattaaagt ggggaaacac attcacatcc tgaacgatcg ccatcgtatg
     1141 tcttcggtgt tcgaacagga tctcggtcaa tggaatgatg gggtaggcaa gcgcttcctc
     1201 aattgccagg tactc
//
LOCUS       DAOPUZ010000417         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_136215_length_1202_cov_3.460331, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000417 DAOPUZ010000000
VERSION     DAOPUZ010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136215_length_1202_cov_3.460331"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /gene="argJ"
                     /locus_tag="PKN79_06520"
     CDS             <1..1121
                     /gene="argJ"
                     /locus_tag="PKN79_06520"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNZ95452.1"
                     /translation="SEAPCTFAGSFTTNLVKAAPVLWDQKVIETSGTVHCILVNSGNA
                     NAATGEKGLRDAQATAALVAGHFGIQTEQTLVCSTGVIGFPLPMETIEKGIASIVKTL
                     DTTNEAALNAAQAILTTDTFTKQVAVEVVVDQKPVRIGGMAKGSGMIHPNMATMLSFI
                     TTDAKISKADLQALLGSSIEDTYNMISVDGDTSTNDTVLVLANGEAENEMLSPSHPDW
                     KAFVKAFDHVNAELAKLIVKDGEGAQKFIEMTVAGAKDKETARTLARSVVSSNLVKTA
                     FFGSDANWGRIFCAMGYSGAQFNPQKTDLFFASEKGKIQVLKDGEPLLFDEREAKRIL
                     SEPVIFCTAVLKDGEASATAWGCDLSYEYVRINGDYRS"
BASE COUNT          290 a          336 c          344 g          232 t
ORIGIN      
        1 actccgaggc tccctgcacc tttgccggca gtttcactac caatctcgtg aaagccgccc
       61 cggtgctctg ggatcaaaag gtgattgaaa caagcgggac ggtccattgc attctcgtca
      121 acagcggcaa cgccaatgcg gcgaccggtg aaaaaggatt gcgcgatgca caagcaaccg
      181 ccgccctcgt cgccggtcac ttcggcattc aaacagagca gacgctggta tgctccaccg
      241 gagtgatcgg ctttccgctg ccgatggaaa cgatcgaaaa agggatcgct tcgatcgtca
      301 aaacccttga taccacgaac gaagcggctc tgaacgccgc tcaggcgatt ctcaccaccg
      361 acacgttcac gaagcaggtg gcggttgaag tagttgttga ccaaaagccg gtccgcatcg
      421 gcgggatggc gaaagggagc gggatgatcc acccgaacat ggccacgatg ctctccttca
      481 tcaccaccga tgccaagata tccaaagcgg atcttcaagc gctcctcggt tcttcaatcg
      541 aggataccta caatatgatc agtgtcgacg gggatacctc gaccaacgac acggttctgg
      601 tcctcgccaa cggcgaagcg gaaaatgaga tgctttctcc ttcccatccc gactggaagg
      661 cgttcgtcaa ggcgttcgat catgtgaacg ccgagctggc gaaactgatc gtcaaggacg
      721 gagaaggggc tcaaaagttc atcgagatga ccgtcgcggg cgctaaggac aaagagaccg
      781 cccggactct tgcccgcagt gtggtctcct ccaatctggt gaagacggcg ttcttcggca
      841 gcgatgcgaa ctgggggcgg atcttctgcg ccatggggta cagcggagct caattcaacc
      901 cgcagaagac ggatctgttc ttcgcttcgg aaaagggtaa gattcaagtc ctcaaagacg
      961 gggagccgct gctctttgat gaacgggagg cgaagcggat cctctcagaa ccggtgattt
     1021 tctgcaccgc ggttctcaag gacggggaag cctccgccac ggcgtggggg tgcgaccttt
     1081 cctacgagta cgtccgaatt aatggagatt atcgaagttg acgacacata cagtagccca
     1141 ggaaattttg aaagcggaaa ccctcatcga ggcgataccg tacattcggc gatttgccgg
     1201 ag
//
LOCUS       DAOPUZ010000418         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_136440_length_1201_cov_2.388307, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000418 DAOPUZ010000000
VERSION     DAOPUZ010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136440_length_1201_cov_2.388307"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /gene="rsmA"
                     /locus_tag="PKN79_06525"
     CDS             complement(<1..641)
                     /gene="rsmA"
                     /locus_tag="PKN79_06525"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNZ95453.1"
                     /translation="MSWTFPYDSPNAITALLEEEGLAMSKKFGQNFLISNSSRSRIVD
                     LLDVQGGSKVWEVGPGIGAITALLLERGVRLTAFELDHGFCRILRTRAFGDEPHFTLI
                     EGDALKTLFTALRETGTPERLCGNLPYNVGSVLIARLLEGESRPERMVFTVQKEVAER
                     ICAGEGSKNWSSFSLLAQADYDVGIGFDISPGSFYPPPKVTSSVVVFQLRSVSR"
     gene            776..1096
                     /locus_tag="PKN79_06530"
     CDS             776..1096
                     /locus_tag="PKN79_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95454.1"
                     /translation="MSNPSHRRSTRTKDEPPIRTRREEIIETLISNPATGVRNIPPAF
                     QLPSRRSVAGAIANTANVPVFHGNAGSIGAIYIIVYATQLRRNASDPTLYGNQRSCKE
                     RASR"
BASE COUNT          320 a          339 c          340 g          202 t
ORIGIN      
        1 cgactcactg atcgaagctg aaacacgacc accgaggagg tgaccttcgg cgggggatag
       61 aaggaaccgg gggaaatatc gaatccgatc cccacatcat agtccgcctg ggcgagcaat
      121 gagaaggagg accaattctt cgatccttca ccggcgcata tccgctccgc cacttccttc
      181 tgaacggtga agaccatcct ctcgggccgg ctttctcctt caagcagccg ggcgatcaat
      241 accgaaccca cattgtaggg aaggtttccg cagagacgct caggggtgcc cgtttctctc
      301 aaggctgtaa aaagcgtctt caaggcatcc ccttcgatga gcgtaaaatg aggttcatca
      361 ccgaacgctc gggtgcgaag gatccggcaa aacccatgat ccagttcaaa ggcggtcagg
      421 cggacccccc gttcaagaag aagggcggtg atcgcgccga tccccggtcc cacctcccag
      481 accttcgatc ccccctgcac atcgagcaga tcgacgatcc tgctccggct cgagttcgag
      541 atgaggaagt tttgtccaaa cttcttgctc atcgccagcc cctcctcctc cagaagggcg
      601 gtgatcgcgt tgggtgaatc gtagggaaag gtccagctca tgggtctcct gcttcaatag
      661 gttcctttta gcatagatga gagcaaagaa gagggtcaac acgaaagcga gcgcgattgc
      721 gtaccctttc cagccaaaaa caaggcttga agaggcgccc caaagaagca ggtcattgag
      781 caatccatca caccgaagaa gcactcggac gaaggacgaa ccgccgatca ggacaaggag
      841 ggaggagatc atcgagacgt tgatcagcaa tccggcgacc ggcgtcagga atattcctcc
      901 ggcattccag ctaccttcaa ggagaagcgt ggcgggggcg atcgccaata ccgccaacgt
      961 tccggttttc cacggaaacg cgggaagcat cggggcgata tatatcatcg tatacgccac
     1021 ccagctgagg agaaacgcca gcgatcccac gctgtacgga aaccaaagaa gctgcaagga
     1081 aagagcaagc aggtaggaat ggagtctgcc gtgacagccg aaaggaagca gggagcagag
     1141 ggccaaccca agagctcgaa tcaaggaagg tttgggaccg gcgatgagca caaacagaca
     1201 g
//
LOCUS       DAOPUZ010000419         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_136596_length_1200_cov_2.767686, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000419 DAOPUZ010000000
VERSION     DAOPUZ010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136596_length_1200_cov_2.767686"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PKN79_06535"
     CDS             <1..342
                     /locus_tag="PKN79_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
                     /protein_id="HNZ95455.1"
                     /translation="EKICLQYYEQRLKNDRVPFLPEDYGNWWGNDPLRKQESEIDILS
                     FNAEAMLFIECKWRNEKTHRKVLAELVEKSKMFKTGRKFYWIISMSGFHDVEHSLINA
                     EMIDLKQVYSV"
     gene            complement(477..>1200)
                     /locus_tag="PKN79_06540"
     CDS             complement(477..>1200)
                     /locus_tag="PKN79_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNZ95456.1"
                     /translation="TSIPASHPEYLAPEIYMRLFSSNSQVFDKNNQVTFSNPRTLKAY
                     IDLIKVMKHVKPNYLQTSDMDAVKDFLLGETAMLITYPSFLYDEELISANPGILNNIA
                     HSNIPGNAPILGGWSLGIFAQSEQKDQAFDFIKWACSDKISDYFALMGGYSAVESTYL
                     NDELTKLYPWFPLYHSTYKTTRPILPPRTASGKIVSQNKIDSIICHGVYELLKDSSDI
                     PSVIEKTHKALEELILSHEETT"
BASE COUNT          282 a          266 c          346 g          306 t
ORIGIN      
        1 gaaaagatat gtcttcaata ctatgagcag cgcttgaaaa acgaccgagt tccgtttttg
       61 ccggaagatt atggaaactg gtggggcaat gatcccctgc gaaagcagga atccgagatc
      121 gatatcctat ccttcaatgc ggaggcgatg ctgttcatcg agtgcaagtg gcgaaatgag
      181 aagactcacc ggaaggtgtt ggcagagctt gtcgagaaat caaagatgtt caagaccggt
      241 aggaagttct attggatcat ttccatgtcg gggtttcatg atgtcgagca ttcattgatc
      301 aatgccgaga tgatcgatct gaagcaggtc tattccgtat agatgaacgg agatcggcga
      361 atactttgtg catcggttcg gtttggccgg catcccgggc cttcagtcct tcttccagaa
      421 ggtcatgcag ctccaccgct ccatcgtatt cgaaagaaga gatgggttta gtcaggtcag
      481 gtcgtttctt catgggaaag aatcagctcc tccaaagcct tgtgggtctt ctcgatgacc
      541 gaggggatgt cgctcgagtc cttcaacagt tcatagactc catggcagat gatcgagtcg
      601 atcttgttct gtgaaacgat cttccccgat gcggttcgcg gcggcaggat cggacgagtc
      661 gttttatagg tcgaatggta gagcggaaac cagggataca gcttcgtgag ctcatcgttg
      721 agataggtgc tttcaacggc cgagtagccg cccatcaatg caaagtagtc gctgatcttg
      781 tcggagcacg cccatttgat gaaatcgaac gcctggtcct tctgttccga ttgggcgaaa
      841 attcccagac tccatcctcc gaggatcggg gcattgccgg gtatgttgct gtgggcgatg
      901 ttgttgagga ttcccgggtt cgccgagata agctcttcat catacaggaa ggaggggtag
      961 gtgatgagca tcgccgtctc gccgagtaga aagtctttca cggcatccat gtcgctcgtc
     1021 tgaagatagt tgggcttgac gtgcttcatg accttgatga ggtcgatata cgctttcaac
     1081 gtccgaggat tgctgaaagt gacctggttg ttcttatcaa acacctgact gttggaagag
     1141 aagaggcgca tatagatttc aggcgccaga tactccgggt gggaggcggg aatcgacgtt
//
LOCUS       DAOPUZ010000420         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_136609_length_1200_cov_2.637555, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000420 DAOPUZ010000000
VERSION     DAOPUZ010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136609_length_1200_cov_2.637555"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PKN79_06545"
     CDS             complement(<1..1169)
                     /locus_tag="PKN79_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HNZ95457.1"
                     /translation="MGSIGIIIGFGVMMGAIFEKSGAATRMAITFIRILGKGREEIAM
                     AITGFIVSIPVFCDSAFVILSPLSKSLSRNTGKSVITISCALGAALLVTHSLVPPTPG
                     PLAVVEFFKLDLGKFILISLLASVPITVAGVFYARWVGTKKVFKLVDPDDSSKLIDRP
                     YTKEEMATTTNISTEGLPSTFISFAPIVFPILLILLNTITSAMGMKGTFKSIAGLIGD
                     PVVAVAIGLLIAIYGLAGKVSRKEVVSMLDGSIREAGVIVFVTGAGGALGQVLKDSGA
                     GAAIANVLVNANIPGILLPFIMAYLLRIVSGSATVGMLTAGSLTAPILLSMANVNPYI
                     GGLACCIGSIGITNLHDSYFWVVNRTTGLTDVKEMFWNWFGAVLVLSTSGFVVLLL"
BASE COUNT          315 a          324 c          315 g          246 t
ORIGIN      
        1 agcagcagta cgacaaaccc ggaagtggaa agaaccagta cggccccgaa ccagttccag
       61 aacatctctt tcacatcggt gagaccggtg gtccggttga cgacccagaa atagctgtcg
      121 tgcaggttgg tgatgccgat tgatccgatg cagcaagcca atccgccgat ataggggttg
      181 acattggcca ttgaaagaag gatcggtgca gtcaacgacc cggcggtcag catgcctacg
      241 gtggccgaac cgctgacgat tctcaacaga tacgccatga tgaacggaag cagaattccg
      301 ggaatatttg cattcaccag aacattcgca atcgctgctc cggccccgct gtccttcaag
      361 acctggccaa gagcaccacc ggcaccggta acgaagacga tcacaccggc ctctctgatc
      421 gatccgtcaa gcattgaaac aacttcttta cgggaaacct tgccagccaa tccatagatg
      481 gcgatgagca gaccgatggc gacggcgacg accggatctc cgataagacc ggcgatgctc
      541 ttgaaggtac ccttcattcc catggcgctt gtgatggtat tcaacaggat cagcaggatc
      601 gggaatacga tgggagcgaa cgaaatgaag gtcgaaggaa gcccttcagt ggagatgttg
      661 gtagtagtag ccatctcttc cttcgtgtag gggcgatcga tcagcttgga agagtcgtcg
      721 ggatcgacca gcttgaacac ttttttcgtt cctacccatc tggcatagaa tactccggcg
      781 accgtgatcg gaacggaagc gagcagactg atgaggatga atttcccgag atcgagcttg
      841 aagaattcaa cgaccgccaa aggtccgggg gttggaggaa ccaatgagtg ggttaccaac
      901 aatgccgcac cgagagcaca actgatcgtt atgacgctct taccggtatt tcgtgacaac
      961 gactttgaca gcggcgaaag aatgacgaat gccgagtcac agaacacagg tatcgatacg
     1021 atgaatcccg ttatcgccat ggcgatctct tcacggcctt ttcccagaat cctgatgaac
     1081 gtgatcgcca ttctcgtggc agccccgctc ttttcgaaga tggcacccat catgacacca
     1141 aatccgatga tgataccaat gcttcccaac gttccgccaa atccactctt gattgccgac
//
LOCUS       DAOPUZ010000421         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_136651_length_1200_cov_2.119651, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000421 DAOPUZ010000000
VERSION     DAOPUZ010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136651_length_1200_cov_2.119651"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PKN79_06550"
     CDS             complement(<1..974)
                     /locus_tag="PKN79_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNZ95458.1"
                     /translation="MRRMLIFLLLPILLLVSCELVQDQPPQGKVYGVFIGLDYRNTTF
                     TNPADEYGRLLPLKGTLNDAREMKTAFGHIGGLAKLKMDSYLMYQEGDTKDQSTYEMI
                     TVGGTPIRSYASKANLALLLDALTDAIKEEDLLILTYHGHGGEDVLFMAPESNDDYDI
                     ELKVTEKLLKELQPIKGRKLVILDSCESGMSVPASESSVSLVRSPSLSDWFDTYFSDE
                     PYAIPPVTLFTATAHTDSYEMGDDHIHGVFTLALLEALGGSHDEDDGFLMGTEIPAKH
                     NGVISVDSLYRYIKQHQKLPLRTTIYRPTSMYQHPMTNGGAMDFILFTL"
     gene            complement(971..>1200)
                     /locus_tag="PKN79_06555"
     CDS             complement(971..>1200)
                     /locus_tag="PKN79_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95459.1"
                     /translation="LFGSGGIAVNRYTAIEWAFLSFWAEAGLQYTILETTNTELSAIL
                     PIMIDLRKEITALSIGLGLRWSFDPYGGGLL"
BASE COUNT          283 a          304 c          331 g          282 t
ORIGIN      
        1 agcgtaaaga ggatgaaatc catcgctccg ccgttggtca tcggatgctg gtacatcgag
       61 gtcggtcgat agatcgtggt ccgaagagga agcttttggt gttgtttgat ataccggtag
      121 agcgaatcga cgctgatcac accattgtgc tttgcaggta tctcagtgcc catgagaaat
      181 ccgtcgtctt cgtcatgaga gccacccagc gcctccagaa gcgctagggt gaagacgcca
      241 tggatatggt cgtctcccat ctcataggag tcggtatgcg ccgtcgcggt gaacaacgtg
      301 accgggggaa tcgcataggg ctcatcgctg aaataggtat cgaaccaatc ggaaagcgag
      361 ggggaacgaa ccagactgac cgaggattcg gaagccggca cgctcattcc actttcacag
      421 gaatcgagga ttaccagttt gcggcctttg atgggctgca attccttgag aagcttttcg
      481 gtaaccttca gttcaatgtc atagtcgtca ttggattcgg gggccatgaa gagcacgtct
      541 tccccgccgt gaccatggta ggtgaggatc agcaggtcct cttctttaat tgcatcggtc
      601 aatgcgtcca acaataaggc gagattcgct ttcgacgcgt atgaacggat tggtgttcct
      661 ccgaccgtaa tcatttcata cgtggattga tccttagtat ctccttcctg atacatgaga
      721 tacgagtcca tcttaagctt tgccagacct ccaatatggc cgaacgccgt tttcatctca
      781 cgggcatcgt tgagggtgcc ctttaggggc agcaagcggc cgtattcatc agcgggattc
      841 gtgaacgtcg tattcctgta atccaacccg atgaacacgc catagacctt tccttgaggg
      901 ggttgatcct gaaccagctc gcaggagaca agcagcagga tgggaagaag gaggaagatg
      961 agcattcttc tcatagcaag cctcctccgt agggatcgaa cgaccagcgt aagcccaaac
     1021 cgattgaaag ggcggttatc tccttcctca ggtcgatcat gatcggaagg atcgccgaga
     1081 gctcggtatt cgttgtttcc agaatcgtgt actgaagacc cgcctccgcc cagaaggaga
     1141 ggaacgccca ttcgatcgcc gtatagcggt tgacggctat tcctccgctg ccaaagagtg
//
LOCUS       DAOPUZ010000422         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_137465_length_1195_cov_3.046491, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000422 DAOPUZ010000000
VERSION     DAOPUZ010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137465_length_1195_cov_3.046491"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PKN79_06560"
     CDS             complement(<1..803)
                     /locus_tag="PKN79_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ95460.1"
                     /translation="MKKTLVLILALVVLGSVLFAQAAAEKKPYIAVVSKGEQHDFWQQ
                     VKLGANAAAKDFNVDITFEGPPSESDVQIQVEMLNNAMAKNPVAIALAALNTSSVLDQ
                     LQETRSKGIPVIGFDSGVPEAPAGSVWATAQTNNYEAAGLAAEKMFEVIKDRIEKSTV
                     AKPVTIVVMNQDASGESLLSRGKGFRDRMVQLIAEKSSLSRDDIAVIGNTAYIAADSP
                     TKGRRVFIEMIVPASAAMTDATNSAQAVLNKVTSHNVLGIFCSNEATIKG"
     gene            complement(856..>1195)
                     /locus_tag="PKN79_06565"
     CDS             complement(856..>1195)
                     /locus_tag="PKN79_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ95461.1"
                     /translation="IVSGFLAGVGGIAFAAVYTTVMPAQGQGFELYAIAGTVIGGTSL
                     SGGSGSIFGTMIGVYIMSVLRAGLPSMDLQSQYQTFFTGVVVLGAVMLDMYRTKKATE
                     VKLPVIDSKE"
BASE COUNT          243 a          326 c          335 g          291 t
ORIGIN      
        1 cccttgatcg ttgcttcgtt ggagcagaag attccgagca cgttgtggct ggttactttg
       61 ttgagcactg cctgtgcgct gttggtggca tcggtcatcg ccgcggaagc gggaacgatc
      121 atttcaatga atacgcggcg acccttggtg ggggagtcgg ctgcgatgta agcggtgttt
      181 ccaatcactg caatatcatc tcttgagagc gagctctttt cggcaatgag ctgaaccatg
      241 cggtcgcgga atcctttacc tcgggagagg agggactcgc cgctggcgtc ctggttcatg
      301 acgacgatcg tgaccggctt ggcgacagtt gatttttcaa tgcggtcctt gatgacttcg
      361 aacatcttct cggctgcaag acctgccgct tcgtagttgt tcgtctgtgc ggttgcccat
      421 accgatccgg ccggtgcctc gggaactccg gagtcgaatc cgattaccgg gatccccttg
      481 gagcgggttt cctgcaattg gtcaagaaca ctgctggtgt tcagcgctgc gagggcgatt
      541 gccaccgggt tcttggccat tgcgttgttg agcatctcaa cctgaatctg cacatcactt
      601 tcagagggcg gtccctcaaa ggtgatatct acgttgaaat ctttggcagc tgcgttcgcg
      661 ccaagcttta cctgttgcca gaagtcgtgt tgctcacctt tggagacaac ggcgatgtac
      721 ggcttcttct cagcagccgc ctgagcgaac aggaccgagc cgagcacaac cagagccaaa
      781 atcaggacta aggttttctt cataagattc ctcctatttt ctcccattcc atacggagtg
      841 gggatttacc gttatttatt cttttgaatc gattaccgga agcttcactt cggtggcttt
      901 cttcgttcga tacatatcca acatcaccgc tccgagaaca acgacaccgg tgaagaacgt
      961 ctggtactgg ctctgcagat ccatcgaggg aaggcccgct cgaaggacgc tcatgatgta
     1021 gacaccgatc atcgtgccga agatcgatcc ggagcctccc gagagactcg ttccaccgat
     1081 gaccgtaccg gcgatcgcgt agagctcgaa gccctgaccc tgagcgggca tgacagtcgt
     1141 gtagacggca gcgaacgcaa ttccgccgac accggcgagg aagccgctga cgatg
//
LOCUS       DAOPUZ010000423         1193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_137845_length_1193_cov_2.947276, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000423 DAOPUZ010000000
VERSION     DAOPUZ010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137845_length_1193_cov_2.947276"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PKN79_06570"
     CDS             <1..746
                     /locus_tag="PKN79_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95462.1"
                     /translation="HMKNLDLRLGRMEFSRFEEQKEIIRTSDPQVLRATYHALLPKLV
                     LGLFRLEGISIEGSELAILYSQSFLPNLSFETQVIALNMRSLLKRMLPSIGLGPIQGN
                     ANPKMSEQRLQHYYAQLLQGLDDDPPAYEERFVIQEGKRHPVSRQMELLFLQYEQEWR
                     YLHPVVRSLFLFGELMRIRPFGRYDWFIASLAFSEEMLKGGYLPTLIGSEEIDEFRSA
                     LLLTNSKGNYQNLLALIEQQQIRAVGRLG"
     gene            complement(750..>1193)
                     /locus_tag="PKN79_06575"
     CDS             complement(750..>1193)
                     /locus_tag="PKN79_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ95463.1"
                     /translation="LRNIVEAQTHVLKRSKEEATEVARTLLAKMGLGEKEKVYPCHLS
                     GGQQQRVSIARALALNPTVLCFDEPTSALDPELTQEILKVIKELAREKMTMIVVTHEM
                     NFARDISDRVIFMDDGLIVEEGSPQELFVNPKNERTKRFLQRYEQ"
BASE COUNT          233 a          329 c          341 g          290 t
ORIGIN      
        1 ggcatatgaa gaacctcgat cttcggttgg gccggatgga gttctcccgc tttgaggagc
       61 agaaggagat cattcgaacg agcgatcctc aggtgctgag ggcgacctat cacgccctgc
      121 ttcccaaact ggtgctcgga ctgtttcgcc ttgaaggcat ctcgattgaa gggtcggaac
      181 tcgcaattct ctacagtcaa agctttctgc cgaacctgag ctttgaaaca caagtgatcg
      241 ccctaaatat gcgctcgctc cttaaacgga tgcttccttc gatcggtttg gggccgattc
      301 agggaaatgc aaatccgaag atgagcgaac aacgccttca gcattactat gctcagcttc
      361 ttcaaggtct tgatgatgat cctcccgcct acgaggagcg attcgtcatc caggagggca
      421 agcgccatcc ggtctcccgc cagatggagc tcctcttcct ccaatacgag caggagtggc
      481 gttaccttca tccggtggtc cgctcgctct ttctctttgg cgagctgatg cggatccgcc
      541 cctttggacg gtatgactgg ttcatcgctt cccttgcgtt cagtgaggag atgctcaagg
      601 gtggttacct gccgaccctt atagggagtg aagagatcga cgagtttcgc tcggcgctgc
      661 ttcttaccaa cagcaaggga aactatcaga accttcttgc cctcatcgaa cagcagcaga
      721 tccgggcggt gggcaggctt ggataaagat tactgttcgt agcgctgaag gaaccgtttc
      781 gtccgctcgt ttttcggatt tacaaagagt tcttgggggg agccttcttc gacgatgagc
      841 ccgtcatcca tgaagatcac ccggtcggag atgtcccggg cgaagttcat ctcgtgggtc
      901 acgacgatca tcgtcatctt ttcacgggcg agctccttga tgaccttgag gatctcctgg
      961 gtgagctcgg gatcgagggc gctggtcggc tcgtcaaagc acagcacggt ggggttcaag
     1021 gcgagcgccc gggcgatgga aacccgttgt tgttgacccc cggagaggtg gcaggggtag
     1081 accttctcct tctcgccgag ccccatcttg gcaaggaggg tccgggccac ttccgtcgct
     1141 tcctctttgc tccgcttcag aacgtgggtt tgcgcttcga cgatgttcct cag
//
LOCUS       DAOPUZ010000424         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_138425_length_1190_cov_2.760352, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000424 DAOPUZ010000000
VERSION     DAOPUZ010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138425_length_1190_cov_2.760352"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1190
                     /locus_tag="PKN79_06580"
     CDS             <1..>1190
                     /locus_tag="PKN79_06580"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HNZ95464.1"
                     /translation="AAGAEYTTYGMDYYDSVLVGGTGDLEWIRALEEARGDEPKIIEE
                     IGCTYLDVMRASLKSEEEFWFEEEKPVVLVSPTWGSHGLLSRYGKAVLQALTEDRKYN
                     IIVRPHPQSFIAEGKLMEELQTTFPDSSNLRWDRRNSGLEAMGQADVMVSDFSGIIFD
                     FLFLFKKPILTFKGIFDKRGRDAMDVDREPWNLEILDRIGRTLGEEDLPHLSAIISAT
                     LQDPVSFEASFQEAQMGMDRYPGESGRRGADFIERTLNTLPRTKEAISKPVSSEPQGW
                     TGKIRMVVSTLFDPSFYLEAFFALLLFYGYLLVGKQILVVDGFNYKFVTQGLPWVTRV
                     LPLPLIGSLALIWIRERGACSFVRTREPFSLKELWLLLFPMAPITQYVIANQDILLFG
                     DSIAV"
BASE COUNT          275 a          306 c          335 g          274 t
ORIGIN      
        1 gcggcgggag cggagtacac gacctacggg atggattact atgattccgt tcttgtaggg
       61 ggaaccgggg atcttgaatg gatccgggcc cttgaagagg caaggggaga tgaaccgaag
      121 atcatcgaag agatcgggtg cacctatctt gatgtgatga gagcatcgct gaagagtgaa
      181 gaggaattct ggtttgaaga agagaagccc gtggtgctgg tatctccaac gtggggaagc
      241 cacggcctgc tctccagata tgggaaagcg gttctccaag ccctcaccga ggacaggaag
      301 tacaacatca tcgtccggcc gcacccccag tccttcatcg ccgaaggaaa gctgatggaa
      361 gagcttcaaa ccacattccc cgattcctcg aacctccgct gggatcgaag aaacagcggt
      421 ctggaggcga tgggtcaggc ggatgtcatg gtcagcgact tctcgggaat catcttcgat
      481 ttcctctttc tcttcaagaa accgattctc accttcaaag gaatcttcga caagcgcggc
      541 cgtgatgcga tggacgtcga tcgcgagccg tggaacctcg agatcttgga ccggatcgga
      601 agaacgcttg gtgaagagga tcttccgcat ctctctgcga tcatcagtgc gacccttcag
      661 gaccccgtct cgtttgaagc atcgttccaa gaagctcaga tgggaatgga tcgctatccg
      721 ggggagagcg gaagaagagg agcggacttc atcgaacgaa cgctgaacac tcttcctcga
      781 accaaagaag cgatttccaa accggtttct tcggaacctc agggatggac aggcaagatc
      841 cggatggtcg tatcaacgct gttcgatcct tctttttatc tggaagcgtt cttcgccttg
      901 ctgctgttct acggatacct gctcgtcgga aaacagattc tggtggtcga cgggttcaac
      961 tacaagtttg tcactcaggg actgccgtgg gtgacccggg ttcttccgct tccactcatc
     1021 ggttcgcttg ccctcatctg gatccgggaa agaggagcat gttcctttgt ccgaacaaga
     1081 gagccgtttt ctcttaagga actttggctt ctgctgtttc cgatggcccc gatcacccag
     1141 tatgtgatag ccaaccagga catccttctc ttcggagatt caatagcggt
//
LOCUS       DAOPUZ010000425         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_138781_length_1188_cov_3.211827, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000425 DAOPUZ010000000
VERSION     DAOPUZ010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138781_length_1188_cov_3.211827"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1188
                     /locus_tag="PKN79_06585"
     CDS             <1..>1188
                     /locus_tag="PKN79_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNZ95465.1"
                     /translation="MPKSSGQFHEVYFGTRTAQIRRLFNYDGFSLVVGVFLIINSLAT
                     AIIASRSLRNGKIYRGTLALSSIELCVGLWVCCGSMSTQLFIHNQLLLVVLAMTALYM
                     LPVCVTWFVVSMYSIPLRRFFMISTLFFPIAFIVVSALQLLGVITYITVLIPLAVLFF
                     FYLLILLGAVMHAYRKGEKNAKPFLVATGFLFVSTVGELTLLILPHPTFINIQPLNIG
                     VLLFGSVLYSQILTQGVHFLEERGRKEYLQSLAYTDALTGLGNRRAFDEELIRLAKSD
                     SKEKQYGIVVFDVNSLKQINDDYGHAEGDLVLREIGELLTSLYGTFATIYRIGGDEFA
                     LICEQCGQERMRDFERLFTEAIAKKPFTLAFGSALSSLDRSDSNDSLYKLADRRMYQQ
                     KKT"
BASE COUNT          254 a          332 c          290 g          312 t
ORIGIN      
        1 acatgccgaa atcaagcggg cagttccatg aggtgtattt cggcacccgg accgcccaga
       61 ttcgtcgtct gttcaactac gacggttttt ctctcgtcgt gggagttttc ttgattatca
      121 actccctggc aacagccatc atcgcctcgc gttcactgag aaacggtaaa atctatcggg
      181 gaacccttgc actgagctcg atcgagcttt gtgtcgggct ttgggtgtgc tgcggaagca
      241 tgtccaccca gctgttcatc cacaatcaat tgctgctcgt tgtcttggcc atgaccgcgt
      301 tatacatgct tccggtgtgt gtcacgtggt ttgtcgtgtc gatgtactcg atcccattgc
      361 gcagattctt catgatcagc acgctcttct tcccgattgc ctttattgtc gtttccgccc
      421 tgcagctgct gggtgtgatc acctatatca ccgtacttat tcctttggcg gttctcttct
      481 tcttttacct gctcatcctc ctcggggcgg tcatgcatgc atatcgtaag ggcgagaaga
      541 atgcaaaacc ctttttagtc gccaccggct ttctctttgt ctcgaccgtc ggcgagctga
      601 ccctcctcat cctcccgcac ccgacgttca tcaacatcca gccgctgaac atcggcgtcc
      661 tcctcttcgg ttcggttctc tacagccaaa tcctcaccca gggagtgcac ttcctcgaag
      721 agcggggaag aaaggaatac ctgcaatcgc tcgcctatac ggatgctctg acgggtcttg
      781 gcaatcgaag ggcgttcgac gaagagttga ttcgacttgc caagagcgat tcaaaagaaa
      841 aacaatacgg aattgtcgtg tttgacgtga atagcctcaa gcaaatcaat gatgactacg
      901 gccacgccga aggagacctc gtcctcagag agatcggcga gcttctcacc tcgctctatg
      961 gaacgttcgc cacgatctat cgaatcggcg gggatgaatt cgccttgata tgcgagcaat
     1021 gcggtcagga gaggatgaga gattttgaac gattattcac cgaggcgatt gcgaagaagc
     1081 cgtttaccct cgcgttcgga tccgctttat catcgcttga ccgttccgac tcgaacgatt
     1141 ccctctacaa gcttgccgat cgacgaatgt atcagcaaaa gaagacga
//
LOCUS       DAOPUZ010000426         1184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_139580_length_1184_cov_2.480071, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000426 DAOPUZ010000000
VERSION     DAOPUZ010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139580_length_1184_cov_2.480071"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..>1184
                     /gene="cobT"
                     /locus_tag="PKN79_06590"
     CDS             196..>1184
                     /gene="cobT"
                     /locus_tag="PKN79_06590"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNZ95466.1"
                     /translation="MDYRKLLLKKAMIPGSLGFLGDLALQLAKFYPRPLDTFSLLLFA
                     GDHGIHAEGVTYSPQEITWQQTRNFASGGGACTLFAKLNGVDLKIIDVGVAGSFDPST
                     SVIDRKVGFGTKNFLHEQAMTDKECASAIEVGRAMTRSSGADLIGFGEMGVANTTSSS
                     AIVAALLDRKPEDVTDGGSGLSEPDLRHKIEVIRKGLAFHTRRDPLDVLSAFGGFEHG
                     AIVGGILEAYTTEKPILLDGFVVGAAALVAAMIDPNVKQNFIFAHRSGMKGSGILLEA
                     LGCTKPILDFGMYLGEGTGALTAWPIVRQASHILWEMSEFEQAEVTDSTAILRS"
BASE COUNT          231 a          336 c          357 g          260 t
ORIGIN      
        1 gaagccgatg agatgattca ccaggaggct cactcccatc gtcgtggcat gccccagatt
       61 ggtgttcgcc accaccatga cggaaatgat cgcaccggtc agcccgtcga gggcgaggta
      121 gattccttga ttcatacggt gagtgtactt cgttacggct tgtcatgcta gtgggcccgc
      181 tctacaatga tgcccatgga ttaccggaag ctcctcctca aaaaagcgat gatacccggc
      241 tctcttggct ttctcggcga cctcgccctg cagcttgcca agttttatcc tcgtcccctt
      301 gatacgttct cccttctgct ctttgccgga gaccatggga tccacgctga aggagttacc
      361 tacagtccgc aggagataac ctggcagcag acccgcaact tcgcttccgg cgggggagcg
      421 tgcaccctct ttgccaagct gaacggggtc gatctcaaaa taatcgatgt cggggtcgcc
      481 ggatcgttcg atccttccac gtcggtgatc gaccggaagg tggggttcgg aacgaagaac
      541 tttctgcatg agcaggcgat gaccgacaag gaatgcgcct ccgccatcga ggtcggcaga
      601 gcgatgaccc gttcatccgg tgccgatctg atcggcttcg gcgagatggg ggtggcgaac
      661 accacctctt catcggcgat cgtcgctgca ttgttggatc ggaagcccga ggatgtgacg
      721 gacggcgggt cggggctcag cgaaccggac cttcgccata agatcgaggt cattcgaaaa
      781 ggtcttgcct tccacacccg acgggatcct ttggatgttc tttccgcttt cggcgggttt
      841 gaacatgggg cgatcgtcgg aggaatcctg gaggcatata cgacggagaa gccgatcctc
      901 cttgacgggt tcgtcgtggg cgccgctgca ttggttgcag cgatgattga tccgaatgtt
      961 aaacagaact tcatctttgc ccaccgttcg gggatgaagg gcagcggcat ccttttggaa
     1021 gccctcggct gcacaaagcc gatcctggat ttcggaatgt acctcggcga ggggacgggg
     1081 gcgctgactg cgtggccgat cgttcgccaa gccagccaca tcctctggga gatgagcgag
     1141 ttcgagcagg cggaggtgac cgattccacc gccattctcc ggtc
//
LOCUS       DAOPUZ010000427         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_141119_length_1176_cov_2.527208, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000427 DAOPUZ010000000
VERSION     DAOPUZ010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141119_length_1176_cov_2.527208"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1176
                     /locus_tag="PKN79_06595"
     CDS             <1..>1176
                     /locus_tag="PKN79_06595"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNZ95467.1"
                     /translation="AIASLFPNTYGASIVSYAKGENSDAKSVRNVGMILSGGQAPGGH
                     NVISGLFDAMQAANPENRLYGFKGGPSGILKDDYRIIDAEYLAAFRNTGGFDIIGSGR
                     TKLETEEQFETCAKVCKKHDISAIIIIGGDDSNTNAAVLAEYFLQKKHDIQVIGCPKT
                     IDGDLKNESIEISFGFDTATKTYAELVGNIARDANSAKKYWHFIRLMGRSASHITLEV
                     ALQTQPNFCLVGEEIGAKNQSLDDVVNELVRIIAARADDGNHFGVGLIPEGVVEFIPE
                     IKVLIQEINTLLAKEEKAFGAIEDDHEKIRFVTDLLSKASAHVFSSFPEAFRLQLLAD
                     RDSHGNVQVSKIETEKLLSSMVEERLNKLGKGAQFQTLHHFFGYEGRCAFPTNFDAD"
BASE COUNT          284 a          342 c          319 g          231 t
ORIGIN      
        1 ggcgatcgcc tccctcttcc ccaacaccta cggcgcatcg atcgtcagct acgcaaaagg
       61 cgagaacagt gatgcaaaat ccgtccgcaa cgtcggaatg atcctctccg gtggccaagc
      121 acccggcgga cataatgtga tcagcggact ctttgatgcg atgcaagcgg caaaccctga
      181 aaacaggctg tacggcttta aaggcggtcc gtcgggaatc ctcaaggatg attatcggat
      241 tatcgacgcc gaatatcttg ccgccttcag aaacaccggc ggttttgaca ttatcggcag
      301 cggtcgcacc aagttggaaa ccgaagagca gttcgagacc tgcgccaagg tgtgcaagaa
      361 gcatgacatc agcgcaatca tcatcatagg cggagatgat tcgaacacca acgccgcggt
      421 gttggccgaa tactttctgc agaaaaagca cgatatccaa gtcatcgggt gtcccaagac
      481 gatcgacggg gacttgaaga acgaatcgat cgagatctcg ttcgggttcg ataccgcgac
      541 gaagacctac gccgagctgg tcggcaacat cgcccgcgat gcgaactccg cgaagaaata
      601 ctggcacttc atccgcctga tgggccgctc agcaagccat atcacgctgg aagtcgctct
      661 tcagacgcag ccgaacttct gcctggtcgg cgaagagatc ggggcgaaga atcaaagcct
      721 agacgatgtc gtcaacgagc ttgtccggat catcgccgcc cgggcggatg acggcaacca
      781 cttcggagtc ggcctgattc ccgagggagt cgtcgagttc attcccgaga tcaaggttct
      841 gattcaggag atcaacaccc ttctcgccaa ggaggagaaa gccttcggtg cgatcgagga
      901 tgaccatgag aagatccgat tcgttacgga tctcctctcc aaggcttctg cccacgtctt
      961 ctcttccttc cccgaagcct tcaggctcca gctgctggcc gaccgcgatt cccacggcaa
     1021 cgtccaggtc tcgaagatcg aaacggagaa gcttctctct tcaatggttg aagagcggtt
     1081 gaacaagctt ggcaagggag cgcagttcca gacgcttcac cacttcttcg gttatgaggg
     1141 ccgctgcgcc ttcccgacca acttcgatgc ggacta
//
LOCUS       DAOPUZ010000428         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_141691_length_1173_cov_2.275492, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000428 DAOPUZ010000000
VERSION     DAOPUZ010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141691_length_1173_cov_2.275492"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKN79_06600"
     CDS             <1..255
                     /locus_tag="PKN79_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ95468.1"
                     /translation="GLAMSGTYPTTLSTMDPRYNASTIVTGTTIATASVGAITMPIIV
                     GSVAEKAGIGSGLATISITIVTMLVLMVVKLLRERPATEG"
     gene            complement(242..991)
                     /locus_tag="PKN79_06605"
     CDS             complement(242..991)
                     /locus_tag="PKN79_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNZ95469.1"
                     /translation="MKLISWNVNGLRAIQQKGFNDYFREADADIFCLQETKLQEHQVT
                     LETPGYYQYWSFAEKKGYSGTALFTKTEPLSVEYGIGHEFDNEGRVINAEFEEFYVMT
                     VYTPNSQEELARLDRRMEWDDAFREYVKALAEHKGVIICGDLNVAHQAIDLKNPKTNE
                     RNAGYTIEEREKFTRLLEAGFVDTFRYLYPDATEAYSWWSYRFQSRSRNTGWRIDYWL
                     VSERLKERVVDSTIATEVMGSDHAPVILTLR"
     gene            complement(1002..>1173)
                     /locus_tag="PKN79_06610"
     CDS             complement(1002..>1173)
                     /locus_tag="PKN79_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HNZ95470.1"
                     /translation="HKRLLANEMPLTIGGGIGQSRLCMYFLKKAHIGEVQASIWPQDV
                     MEEAASLGVVLM"
BASE COUNT          247 a          337 c          289 g          300 t
ORIGIN      
        1 ggtctggcga tgagcgggac ctatccgacc actctttcaa cgatggatcc acgctataat
       61 gcatcgacga tcgtaacggg tacgacgatt gcgactgcat cggtcggggc gatcacgatg
      121 cctataatcg tgggttcggt cgccgagaaa gcgggaatag gttcggggct tgccacgatc
      181 tccataacga tcgttacgat gcttgtgctg atggtggtga agctcctcag ggaacgaccg
      241 gctaccgaag ggtaagaatc accggcgcat ggtcgctacc catcacctcg gtggcgatcg
      301 tcgaatcgac cacccgttct ttcaagcgtt cgctcaccag ccaataatcg attctccacc
      361 cggtattccg tgatcggctt tgaaagcgat acgaccacca cgaataggct tcggttgcat
      421 ccggatacag atagcggaag gtgtcgacaa atccggcttc cagcagccgg gtgaacttct
      481 cccgttcttc gatcgtatag ccggcgttgc gttcattcgt tttcgggttt ttcaagtcga
      541 tcgcctgatg ggctacattc aaatcgccgc agatgatgac gcctttgtgt tccgccaacg
      601 ctttgacata ttctcgaaac gcatcatccc actccatccg ccgatccaag cgggcaagct
      661 cttcctgact gtttggagta tagaccgtca tcacgtagaa ctcttcgaac tcggcgttga
      721 tgactcgtcc ttcattgtcg aactcatgtc cgattccata ttcgacggaa aggggttccg
      781 tcttggtgaa caacgcagtt ccgctatagc ctttcttctc ggcaaatgac caatactgat
      841 aatagccggg ggtttccaga gtcacctggt gttcctgaag cttcgtctcc tgcaggcaga
      901 agatatccgc atccgcctcc ctgaaatagt cgttgaagcc tttctgctgg atggctctca
      961 atccgtttac gttccaactt atcagtttca ctgcttcctt cctacatcaa tacgacgcca
     1021 aggcttgcgg cttcttccat cacatcctgc ggccaaattg atgcttgcac ctctccgata
     1081 tgggctttct tcaggaaata catgcagagg cggctttgac cgatcccgcc tccgatcgta
     1141 agcggcatct cattggccag cagccgcttg tgc
//
LOCUS       DAOPUZ010000429         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_142051_length_1171_cov_2.467742, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000429 DAOPUZ010000000
VERSION     DAOPUZ010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142051_length_1171_cov_2.467742"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKN79_06615"
     CDS             complement(<1..247)
                     /locus_tag="PKN79_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95471.1"
                     /translation="MSDFRTTPGNLVRARGRDWVVQAGSDETVLVLRPLRGGDDVVYI
                     ASDLEQEPVRAAVFDDPKPSQLSNMRSGTLFREALMLK"
     gene            complement(259..>1171)
                     /locus_tag="PKN79_06620"
     CDS             complement(259..>1171)
                     /locus_tag="PKN79_06620"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNZ95472.1"
                     /translation="RRRSDKEILGFLIDPLSGLWSKSENEDQDEPENPEVPVTSKVKP
                     QRIVPYVEDHKNIMVFHPEGELSFTAMVTLQAALKRGIEQVFQIEESELAVEPLPTNE
                     VRKRILFYEAVEGGAGVLNRLVEEEKALSIVADQALRIMHYVNDQELWDADAMQEVVD
                     EKGNPPCEAGCYQCLLSYYNQPEHLGIDRRDSDAIRILVALANGSVSKRDDQAPLEVS
                     EVASEFQEALKKRNLKLPDRSSVTIKDGSVLPLLYTSARTAVFFGMASEATKRYCADR
                     SITILEVDTDERSWDEMFRAHSDVFGE"
BASE COUNT          270 a          360 c          266 g          275 t
ORIGIN      
        1 gcttcagcat caaagcctcc cggaacaggg taccggaacg catgttcgaa agctgggagg
       61 gctttggatc atcaaagacg gctgcccgga caggctcctg ttcaagatcg gaagcgatgt
      121 acaccacatc atcaccgcca cgcaacggcc tgagaaccaa taccgtctca tcgctgccgg
      181 cctgaaccac ccagtctctt cctcttgcac ggacaagatt gccgggagtt gtacggaaat
      241 cactcatctc tcaattcctc attcgccgaa cacatcggaa tgcgctctaa acatctcatc
      301 ccaagacctc tcatccgtat cgacctccag gatagtgatg gatctgtctg cacagtaccg
      361 cttggtcgct tcactggcca taccgaaaaa gaccgccgtt cttgcagaag tatacagcag
      421 gggaagcact gaaccgtcct tgatggtcac cgagcttcga tcgggaagct tgagattcct
      481 tttcttcaac gcctcttgga actccgacgc tacttctgaa acctccaaag gcgcctgatc
      541 atcccgcttg gagacggaac cattggccag tgccaccaag attctaattg catcggaatc
      601 ccttcgatcg atacccaaat gttcaggctg attgtaatag gacagcaggc attgatagca
      661 ccccgcctcg caaggaggat tccccttctc atcaacgacc tcctgcatgg catcggcatc
      721 ccacagctcc tgatcattca catagtgcat gatcctcaac gcctggtcgg ccacgatcga
      781 aagagctttc tcctcctcga ccagtctgtt caatacaccg gctccccctt ccaccgcttc
      841 gtagaaaaga atcctctttc ggacttcatt ggttggaagg ggttctacgg ccagctccga
      901 ttcttcaatc tggaacactt gttcaatgcc gcgtttcaga gccgcctgca gggtaaccat
      961 ggcagtaaag gaaagttcgc cttcggggtg gaacaccatg atgttcttgt ggtcttccac
     1021 atagggaacg atcctctgag gcttcacctt actggtaacc ggtacttcgg ggttctccgg
     1081 ctcatcctgg tcctcattct ccgacttgct ccaaagcccg gacaagggat cgatcaggaa
     1141 tccgagaatc tccttatcgc ttctgcgtct c
//
LOCUS       DAOPUZ010000430         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_142393_length_1169_cov_3.159785, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000430 DAOPUZ010000000
VERSION     DAOPUZ010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142393_length_1169_cov_3.159785"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PKN79_06625"
     CDS             complement(<1..738)
                     /locus_tag="PKN79_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HNZ95473.1"
                     /translation="MADLSSTYLGLPIRNPFVAASGPLTASLDSLKRLEDAGLGAVVL
                     KSIFEEQIELDGMTALKNAESYLGHTDGYEFVKATSMEQQIDAYLGLLEGAKKSLAIP
                     VIASINCYRDGTWLEYAKRFIDLGADAIELNYYVVAADPETEGREMEKEFLQLVRSAR
                     KSIDVPLSLKIGCAYSSLANLLRRFDEEEVDGVVLFNRFYSPDIDIEHMKITQAQMIS
                     SKDEYALTLRWVGLMSEEVRYDLIGATG"
     gene            complement(829..>1169)
                     /locus_tag="PKN79_06630"
     CDS             complement(829..>1169)
                     /locus_tag="PKN79_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNZ95474.1"
                     /translation="RVNKLIDEEVLQISGLIRSEMIPGLQVVFMGVKLSTLDLERKAK
                     EFCTLRGVISSVVVTGRYDLIVQLVLSEEEDLSLLDFFKEELSKMSEVLEVETFVVYQ
                     SANYRIPYVL"
BASE COUNT          282 a          321 c          283 g          283 t
ORIGIN      
        1 tcccgtagcg ccgatgaggt cgtaccgaac ctcttcactc atcaaaccga cccacctgag
       61 ggtaagggca tattcatctt tgctactgat catctgcgct tgagttatct tcatgtgctc
      121 aatatcgatg tcgggactgt agaagcggtt gaacagaacg accccgtcca cctcctcttc
      181 atcgaagcga cgaagcagat tcgccaatga ggaataggcg cacccgatct tcagactgag
      241 cgggacatca atcgacttcc gggcggagcg gaccagttgg agaaactcct tttccatctc
      301 cctgccctct gtttcaggat cggcagcaac cacatagtag ttcaattcaa tcgcatccgc
      361 tccgagatcg atgaagcgtt ttgcatattc cagccatgtt ccatcccggt agcagttgat
      421 cgaagcgatg accgggatgg caaggctctt cttggctcct tcaagcaagc ccaggtatgc
      481 atcgatctgt tgttccatcg aggtggcttt cacgaactca tagccatcgg tatggccaag
      541 gtagctttcg gcatttttga gggcggtcat accgtcaagc tcgatctgct cttcgaagat
      601 cgatttcagg acaacggcgc caaggccggc atcttccaat cgtttcaggg aatccagtga
      661 tgcggtcaac gggcctgaag ctgctacgaa ggggttcctg atcggtaatc caaggtaagt
      721 actggacaaa tctgccatcg ggtatcttct cctcaatcca aaggtattca tcaaaccaag
      781 acggggcaag tataccatac ctgccccgat tcctctaccc ggtttcgctc agagcacgta
      841 tggaattcgg tagtttgccg attgatagac gacgaatgtt tcgacttcca gaacctcgct
      901 catcttggaa agctcctctt tgaaaaaatc gagcagactc agatcctctt cttcgctgag
      961 aaccagttgg acgataagat cgtaacgccc cgtcaccaca acggaagaga tgacaccgcg
     1021 caacgtgcaa aactcctttg cttttcgttc cagatcgagc gttgacagtt tcactcccat
     1081 gaagacgact tggagaccgg gaatcatttc ggagcggatc agaccggaga tctggaggac
     1141 ttcttcatca atcagcttgt tgacgcgcg
//
LOCUS       DAOPUZ010000431         1164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_143422_length_1164_cov_3.080252, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000431 DAOPUZ010000000
VERSION     DAOPUZ010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143422_length_1164_cov_3.080252"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKN79_06635"
     CDS             <1..489
                     /locus_tag="PKN79_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNZ95475.1"
                     /translation="GLALFGDRNQSFGVRMKQVAVHPCMIAVYLGFLLMFTSLALPSF
                     VTKTLASLGNSTTALSMLLIGSLLAEPGQNPFHADRSLLHFTVLRLLVIPLASFLICR
                     MIGIDPVITGAGVILAAMPGGSTTVILASKYGSDTAYASKLVVFSTLLSLLSIPLWGM
                     VL"
     gene            complement(414..>1164)
                     /locus_tag="PKN79_06640"
     CDS             complement(414..>1164)
                     /locus_tag="PKN79_06640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNZ95476.1"
                     /translation="VHPYISAYLAANIPFLALALGLILSHSAVLKEPLIGLVTETNAW
                     SWRLALLAAGSYLAVTALFTVISALRYPQDYHALRIPLLKRLGFIATALIITPIQTTG
                     EEILFRIIPLKLMKKIQSKAIKALVPTLIFTLLHLANREIAQSQNRLAVLLYYALFGF
                     IPSYIAVRSDRYEFPLAIHAANNLFVVLFCNYEHSSLPSLPLFTSTRPVGTWMDILQL
                     TAALAVSYSTIPQRGMESNESSVEKTTSLDA"
BASE COUNT          245 a          298 c          360 g          261 t
ORIGIN      
        1 gggcttgcgt tgttcggcga ccggaatcaa tcgttcggcg tccgaatgaa acaggtggct
       61 gtccacccgt gcatgatcgc tgtgtatctc ggctttctct tgatgttcac ttcccttgcc
      121 ttgccgtcgt ttgtcactaa aacgctcgct tcgctgggca acagcacgac cgccctttcg
      181 atgctgctga tcggctccct gcttgccgaa ccgggacaaa accccttcca tgccgatcgg
      241 tcgctgctcc atttcaccgt gttgaggctc ttggtcatcc cgcttgcctc gtttctcatc
      301 tgccggatga tcggcatcga tccggtcatc accggggcag gggtgattct tgccgcgatg
      361 ccgggcggat cgacgaccgt cattctcgca tcaaagtatg gaagtgacac cgcctatgca
      421 tccaagctcg tggtcttttc gacgctgctc tcgttgctct ccattcccct ttgggggatg
      481 gtgctgtaac tgaccgccaa tgccgccgtc aactgaagga tatccatcca tgtgccgacc
      541 ggtcgggtgc tggtgaagag cggcagggat ggaagcgacg agtgttcata gttgcagaag
      601 aggacgacga agagattgtt ggcggcatgg atcgccaacg ggaactcgta gcgatcgctt
      661 cgtacagcga tataggaagg gatgaagccg aacagagcgt agtagagaag gactgcaagc
      721 cggttttggc tttgcgctat ttcgcgattg gccaaatgga gaagtgtaaa gataagggtg
      781 ggaaccagag ccttgattgc cttgctttgg atcttcttca tcagtttcag cgggatgatc
      841 cgaaagagga tctcctcgcc ggtggtctgg atcggagtga tgatgagggc tgtggcgatg
      901 aagcccagcc gcttcaggag cgggatgcga agcgcgtggt agtcctgagg atagcggagt
      961 gcactgatga cggtaaagag cgcagtcacc gccagataag atcccgccgc caagagggca
     1021 aggcgccaag accacgcgtt tgtttcagta acaagaccga tgagcggttc tttcagaaca
     1081 gccgagtggg agagaatcaa tccgagggca agggccaaga aggggatgtt tgcggcaaga
     1141 tacgccgaga tgtagggatg cacc
//
LOCUS       DAOPUZ010000432         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_143906_length_1162_cov_2.195122, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000432 DAOPUZ010000000
VERSION     DAOPUZ010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143906_length_1162_cov_2.195122"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PKN79_06645"
     CDS             <1..966
                     /locus_tag="PKN79_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ95477.1"
                     /translation="NMEKARQFAQKWKIKAYTDLDQMVKEAKVDAVVVTTVHPLHMEH
                     TLQALDAGLHVMVEKPMALTVRQCDLMIEKAEEKHKKLGVISQRRWYPACQRIRKAID
                     SGNLGTPVIGQVTMLGWRDKAYYDSDPWRESWELEGGGVLINQAPHQLDLLHWYLGEI
                     AEVYAQWDNFNHPYIEVEDSAVATVRFKSGAIASILVSNSQKPGIYAKVHVHGSNAFS
                     AGVQTDGGAMFVAGQSTITDPAVNDLWTIEGEQGNLERWKEEDAKLFREVDATTYFFT
                     LQLEDFTQAILEDRDPASSGYDGRETVKLIEGIYQSGRTGKPVRY"
     gene            978..>1162
                     /locus_tag="PKN79_06650"
     CDS             978..>1162
                     /locus_tag="PKN79_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNZ95478.1"
                     /translation="MHQELQEIVYEANMALKRNGLITLTWGNVSMVDAKREVMAIKPS
                     GVPYESLRPSDIVLLNL"
BASE COUNT          282 a          319 c          358 g          203 t
ORIGIN      
        1 aacatggaaa aagcccgaca gttcgcacag aagtggaaga tcaaagcgta tacggacctt
       61 gatcagatgg tcaaagaggc gaaagtcgat gcggtggtgg tgaccaccgt ccacccgctt
      121 cacatggagc acactcttca agcccttgat gccggccttc acgtcatggt tgaaaaaccg
      181 atggccctga ccgtccggca gtgcgatctg atgatcgaga aagcggagga gaagcacaag
      241 aagctcgggg tgatcagcca gcgccggtgg tatccggcgt gccagcggat ccgcaaagcg
      301 atcgacagcg gaaatctcgg cactccggtc atcgggcagg tgacgatgct cggatggcgg
      361 gacaaggcgt attacgacag cgacccgtgg cgggaatcgt gggagctgga gggcggcggc
      421 gtcctgatca accaagcccc ccaccagctg gatcttcttc attggtatct cggggagatc
      481 gcagaggtct acgcccagtg ggacaacttc aaccacccct acatcgaagt ggaggacagc
      541 gcggtggcga cggtccgctt caaaagcggt gcgatcgcct cgatcctcgt ctccaactcg
      601 caaaagccgg ggatctacgc caaggtccac gtccacggct ccaatgcgtt ctcagccggc
      661 gtccagacgg acggtggggc gatgttcgtg gcgggacaga gcacaatcac cgacccggcg
      721 gtaaacgacc tgtggactat cgaaggagaa cagggtaatc tcgaaagatg gaaagaggag
      781 gacgccaagc tctttcgtga agtggatgcg accacctact tcttcaccct gcagcttgag
      841 gacttcaccc aagcgatcct ggaagacagg gatcccgctt cctcgggata tgacggtcgg
      901 gagacggtga agctgatcga agggatctac cagagcggcc gcaccggaaa gccggttcgg
      961 tattaaagga gcgagcaatg catcaggaac ttcaggagat cgtatacgaa gccaatatgg
     1021 ccttgaagcg caacggcctc atcaccctca cttgggggaa cgtctcgatg gttgatgcca
     1081 aacgggaagt gatggcgatc aagccttcgg gcgttccgta tgaatcgctg cgcccgagcg
     1141 acatcgtgct tcttaacctt aa
//
LOCUS       DAOPUZ010000433         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_144001_length_1161_cov_3.403255, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000433 DAOPUZ010000000
VERSION     DAOPUZ010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144001_length_1161_cov_3.403255"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKN79_06655"
     CDS             complement(<1..469)
                     /locus_tag="PKN79_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNZ95479.1"
                     /translation="MIKAGQIEKGTALLIKGQPFVVVEREFVNPGKGSAFVRVKLKSP
                     ATGQVLQETMKTQDNVEDITVADRDCQYLYNDGEHFHLMLTDTFEQLEVPMSSFPEYE
                     LLMQDGEVYRCTFWEAELLDIIIPAKVVYLVAEAEEAVKGDTVQGATKYVTTET"
     gene            complement(466..>1161)
                     /locus_tag="PKN79_06660"
     CDS             complement(466..>1161)
                     /locus_tag="PKN79_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HNZ95480.1"
                     /translation="MLEYYSVGFDEEDSILLTEELFRTLEVPKRLQPPFRRMSVAEAT
                     KRYAGFDLEKTQRRSLLKEEALRLGLSVPDEPESWEETFNRIFLTFVEPNLPQEKPLV
                     LYDYPRQIECLAQVNGAYRRRWELYGMGMELANCYYEEADEQKIEAYYRDECARLIQI
                     REIGGGVIADVDPTLAKTFAGIGKVSGVALGLDRLLMGMMGRSSLEGVILFPLSAILS
                     AGNRNLYHDEEYI"
BASE COUNT          245 a          338 c          298 g          280 t
ORIGIN      
        1 cggtctccgt ggttacgtac ttggtcgccc cttgaaccgt atcgcccttc accgcttcct
       61 ccgcttcggc gacgaggtag acgaccttcg ccgggatgat gatatcaagc aactccgctt
      121 cccagaacgt acagcgatag acttctccat cctgcatcag aagttcatac tccggaaagg
      181 agctcatcgg gacttcaagt tgctcgaacg tatcggtgag catgaggtgg aagtgttctc
      241 cgtcgttgta caggtattgg cagtcgcggt cggcaaccgt gatgtcttcg acgttgtcct
      301 gggttttcat tgtttcttgg agaacctgtc cggtagccgg gcttttcagc ttcacccgaa
      361 cgaacgccga accctttccg gggtttacga actcacgctc gactacgaca aagggttggc
      421 ctttgatcag caatgcggtc cctttttcaa tttgacctgc tttaatcata tgtattcctc
      481 atcatggtaa agattcctgt tgccggcgct caggatagca gaaaggggaa aaagaatcac
      541 cccctccagc gaagagcgac ccatcattcc catcagcagg cggtcgagcc ccaaggcgac
      601 gccgctgacc tttccgatcc cggcgaaggt tttggcaagt gtcgggtcga catcggcgat
      661 gacgccgccg ccgatctccc tgatttgaat cagacgggca cactcatccc ggtaataggc
      721 ttcgatcttt tgttcatccg cttcctcgta ataacagttg gccagctcca tccccattcc
      781 gtacagctcc caacgccttc gataagctcc gtttacctga gccagacact cgatctgacg
      841 gggatagtcg tacagcacaa ggggcttttc ctgagggagg ttcggttcga cgaacgtcag
      901 gaagatccga ttgaacgtct cttcccagct ttccggctca tcggggacgg agagcccgag
      961 acgaagggct tcttctttga gcaaggatcg gcgctgagtc ttctcaagat cgaagccggc
     1021 gtatcgtttc gtcgcttcgg cgacgctcat cctcctgaag gggggctgaa gcctctttgg
     1081 aacctccaat gtacgaaaga gctcctccgt cagaagaatc gaatcctctt catcgaagcc
     1141 gaccgagtaa tactccagca t
//
LOCUS       DAOPUZ010000434         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_144434_length_1159_cov_2.899457, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000434 DAOPUZ010000000
VERSION     DAOPUZ010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144434_length_1159_cov_2.899457"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKN79_06665"
     CDS             <1..576
                     /locus_tag="PKN79_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HNZ95481.1"
                     /translation="EQIHVNTWAQEPIRLHLPRIVEGYASDGSPLFQAKTFWAVIDLK
                     NSRPLRPQEMNERIGLLKDPSRAIDMNLPKSTVDFETLYQLGSYAPRVTYLDTDRNQH
                     INNVSYLSWVLEALPAEFRTTYKVKSADVLYQKQTFLDDLLFVHTYAADEEGYNEETP
                     SLVSTIVRKTADGTEEIVFTAHTEWKKRSEF"
     gene            complement(573..>1159)
                     /locus_tag="PKN79_06670"
     CDS             complement(573..>1159)
                     /locus_tag="PKN79_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95482.1"
                     /translation="GGETADGLGRPRVSRPQLFKDGGVYTLFFGASHIVLEDSRHKSS
                     RYFASATSSDLLGPYRLANHGRPLIEPDGDDPNRSLAVGSVRVLKASDGYVALQCGKY
                     WDRELKKSSTSLIQLESVDGVNWLPSTRPRILSPPSEGWASRYIISCDARYKEDEGCV
                     YCYFSASSKRGFRPVKEAIGLLLGKDPVLRKVFL"
BASE COUNT          262 a          351 c          314 g          232 t
ORIGIN      
        1 gaacagattc atgtgaacac ctgggcccag gagccgatcc ggcttcacct gccccggatc
       61 gtcgaaggct atgcctccga cggttctccg ctctttcagg cgaagacctt ctgggcggtc
      121 atcgacctga agaactcccg ccctcttcgg cctcaggaga tgaacgaacg gatcggattg
      181 ttgaaagatc cctcccgagc catcgatatg aatctcccga agtcaaccgt cgattttgaa
      241 accctttatc aactgggaag ctatgcgccg cgggtcacct atcttgatac cgaccggaat
      301 cagcacatca acaacgtctc ctacctctcg tgggtccttg aggcgctgcc ggcggagttc
      361 cgaacaacct acaaggtcaa aagcgcggat gtgctctacc agaagcagac cttccttgac
      421 gatctgctct tcgtccatac ctatgcagcc gatgaagagg gatacaacga agaaacgccc
      481 tctctggtca gcacgatcgt ccgaaagacg gccgacggca ccgaagagat cgtcttcacc
      541 gcccacaccg aatggaagaa gcgaagcgag ttctagagga agacttttct gaggacggga
      601 tcctttccca agagcaggcc gatcgcctct ttgaccggtc gaaatcctct cttggagctt
      661 gcgctgaagt agcagtacac gcatccttca tcctctttat agcgggcatc gcatgaaatg
      721 atatagcggc tcgcccaccc ctctgaagga ggactcagga tcctaggccg tgttgaaggg
      781 agccaattca ccccgtcgac ggattcaagt tgaataaggc tggtggagct tttcttcaac
      841 tccctatccc aatacttccc gcattggagg gcgacgtacc cgtcgctcgc cttcaggacc
      901 cggacggaac cgacggcaag cgatcggttc gggtcatctc cgtccggttc gatcaagggc
      961 ctgccatggt tggccaaccg gtacggacca aggaggtctg aagaggtcgc cgaagcgaag
     1021 tagcggctcg atttatgcct gctgtcctca agcacgatgt gcgaggcgcc aaagaaaagg
     1081 gtgtagactc ctccatcctt gaacagctga gggcgggaga cccggggccg gcccaggccg
     1141 tcggccgtct cgcccccta
//
LOCUS       DAOPUZ010000435         1152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_145862_length_1152_cov_2.206016, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000435 DAOPUZ010000000
VERSION     DAOPUZ010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145862_length_1152_cov_2.206016"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /gene="cmk"
                     /locus_tag="PKN79_06675"
     CDS             complement(<1..545)
                     /gene="cmk"
                     /locus_tag="PKN79_06675"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNZ95483.1"
                     /translation="MIVAIDGPAGVGKSSIARMLAEKSTFTYLNSGSFYRAYTYLHQQ
                     KGRDVGDLAGVLSTAKETHLRLVDGRLHADGIDIEDKLRTKEVDSVVAQVSAYPPLRS
                     YVNEQLKAIAQGEDMIVEGRDITTVVFPDAELKIYFDAKPEIRAERRFRQHPEGTTYE
                     EVLAGVKARDEIDRNKAVGAL"
     gene            complement(542..>1152)
                     /locus_tag="PKN79_06680"
     CDS             complement(542..>1152)
                     /locus_tag="PKN79_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNZ95484.1"
                     /translation="LGTKVNEDDVVMVDGRTIEPVEQYFYFALNKPRGYVSTNYDPNE
                     RNFARDLLDVPQKPLLFSIGRLDKDSSGLLLFTNDGELTQKLSHPSHEVQKEYLVSAS
                     RPIAREDLEAMRRGVYLPDNRLAYRIHRFEILGSRWTRIILTEGKNREIRKLLEHFDY
                     RVLDLIRVRVGPVELGELKTGHWRRLTSDEVRRLHDEKGQKR"
BASE COUNT          234 a          320 c          306 g          292 t
ORIGIN      
        1 tttaaggcac ccacagcctt gtttcgatcg atttcatcgc gggctttgac cccggcaagc
       61 acctcctcat aggtcgtccc ttcaggatgt tgcctgaagc ggcgttcggc tcgaatttcc
      121 ggctttgcgt cgaagtagat cttcagttcg gcatcgggaa aaacaaccgt ggtgatatcc
      181 cgcccttcga cgatcatgtc ttctccttga gcgatcgcct tcagctgttc attcacatag
      241 gagcgcagcg gaggatatgc cgatacttga gcgacaaccg aatcgacttc cttggtccgc
      301 agcttgtctt cgatatcgat cccatcggcg tggagacgtc cgtcgaccaa ccggaggtgc
      361 gtctctttgg ccgtggaaag aactcctgca agatcgccga catccctccc cttttgttgg
      421 tgaagatagg tataggcgcg ataaaaagaa ccgctgttca ggtacgtgaa ggtgcttttt
      481 tcagcaagca tccgggcgat tgaggatttt ccgaccccgg cgggtccatc gattgccacg
      541 atcatcgttt ttgtcctttt tcatcgtgaa ggcggcgaac ttcatccgaa gtaagtcggc
      601 gccagtgtcc tgttttcagt tctcccaatt caaccggtcc aacgcgaacc ctaatcaggt
      661 ccagtacccg gtaatcaaaa tgttcaagca atttccggat ttcacggttt ttcccctcgg
      721 tgagaatgat tcgggtccat cgagacccca gaatctcaaa tcggtgtatc cggtaggcaa
      781 gccgattgtc gggcagatat actccccggc gcatcgcttc cagatcctct cgggcgatcg
      841 gccgggaagc gctgaccaag tactcctttt gcacttcatg ggaaggatgg ctcagcttct
      901 gagtcagctc gccgtcgttg gtgaagagga gcagcccgct ggaatccttg tcgagccggc
      961 caatgctgaa cagcagcggt ttttgcggga catccagcag atctctggcg aagttccgtt
     1021 cattgggatc atagttggtg ctcacgtagc cgcggggttt gttgagggcg aagtagaaat
     1081 attgctccac cggttcgatc gtccgaccgt cgaccatgac cacatcgtcc tcattgactt
     1141 tcgttccgag gg
//
LOCUS       DAOPUZ010000436         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_145986_length_1151_cov_3.209854, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000436 DAOPUZ010000000
VERSION     DAOPUZ010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145986_length_1151_cov_3.209854"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..>1151
                     /locus_tag="PKN79_06685"
     CDS             167..>1151
                     /locus_tag="PKN79_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNZ95485.1"
                     /translation="MNGSFILIGSFLALLIFKFPITFSLFLSSIITATYLNIPLMSIV
                     QRLVSGVHSFSLLAIPFFILSGEIMSQGGISRRLVHFSNALVGQIRGGLAQVNIIASM
                     LFGGISGSAVADVSSIGAMLIPMMVKDGYDADFSVAVTVTSSLESLIIPPSHNLIIFA
                     MAAGGGVSVGKLFLGGIVPGIGLGLLLMIPTAIISVKRNYPKMERMAFKEIIKVAVDA
                     FFGLMTIIIIIGGVISGIFTATESAAVATVYAFIVTFFVYREIPIRQMSSILNNVLRT
                     LAMVMSLIAAASSFGYLMAYLRIPQMVTNALLTVTTNRVALLLLINLMLLIL"
BASE COUNT          212 a          334 c          325 g          280 t
ORIGIN      
        1 acggctggaa gcttgcggtt cacgggatgc gctcgcgcct gcctgcgacc cagctgccca
       61 atatggtcaa ctacgccgtg cttccgatcg ccggggtgtt catcgtcctg tatgcggcca
      121 tccatctcgt tgaagagatc cagcgtttca agaaaggagg tgcagcatga atggatcgtt
      181 tattttaata ggctccttct tggcattgct gatcttcaaa ttcccgatca ccttctcact
      241 ctttctctcc tcgatcatca cggcgacgta tctgaacatt cccttgatgt cgatcgtcca
      301 gcgcctcgtc agcggggtgc actccttcag ccttctggcg attcccttct tcatcctaag
      361 cggggagatc atgagccaag gggggatcag tcggcgcctg gtccacttct ccaacgcgct
      421 ggtggggcag atccgcggag gtctcgccca ggtaaacatc atcgcctcga tgctcttcgg
      481 cggaatcagc ggttcggcgg tcgccgacgt ctcttcgatc ggggcgatgc tgattccgat
      541 gatggtcaag gacgggtacg atgcggactt ctccgtagcc gtcactgtca cgagcagcct
      601 ggagagcctg atcattcccc ccagccacaa cctgatcatc ttcgcgatgg ccgcaggcgg
      661 tggagtatcg gtcgggaagc tgttcctcgg aggaatcgtt cccgggatcg gcctcggctt
      721 gctgctgatg attccgaccg cgatcatctc ggtgaagcgg aactatccga agatggagcg
      781 gatggcgttc aaggagatta taaaagtcgc agtcgatgcg ttcttcggcc tgatgacgat
      841 catcatcatt atcggaggcg tcatcagcgg gatctttacc gcaacggaaa gcgccgccgt
      901 ggcgacggtg tacgcgttca tcgtcacgtt cttcgtctat cgggagattc cgatccggca
      961 gatgagttcg attctgaaca acgtgcttcg cacccttgcg atggtgatga gcctcatcgc
     1021 cgcagcgtcc tccttcgggt atctgatggc gtacttgagg attccgcaga tggtcaccaa
     1081 tgcgctcctc acggtaacca ccaatcgggt ggcgctcctc ctcttgatca acctgatgct
     1141 gttgatactg g
//
LOCUS       DAOPUZ010000437         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_146075_length_1151_cov_1.963504, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000437 DAOPUZ010000000
VERSION     DAOPUZ010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146075_length_1151_cov_1.963504"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1151
                     /locus_tag="PKN79_06690"
     CDS             <1..>1151
                     /locus_tag="PKN79_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95486.1"
                     /translation="KEHGPAPLPKIAESLKLEQSEVGSSFGLLSKGGFVGMNEANNAE
                     AKARELDGMILLTRTVLKKGLDGLLDDDTLTSEERTAVSRIAKKRGAATSPFKVVERE
                     EVTYTLTEAGLKAKEAIIALKITGEELGLVTSEILSTGSWKNATFRPYALNAPTSRLI
                     VGRHNAYADYLQWVKNKLISLGFEEFDGSLVESEFWNGDALFMPQFHSARDIHDVYYV
                     KEPRHAKTIEEPWLSQVAATHENGWKTNSRGWGYTFDREFTRRQILRSQGTVLSARQL
                     PKAKVPGKYFGVVRCFRYDQVDATHGADFYQTEGIVLGEEVNLKNLLGLLKMFAEEIA
                     GAEEGVLLAFERRHNFASAWKGLSLPSLTLVRNGEVCWVCADGRAPIRA"
BASE COUNT          247 a          338 c          363 g          203 t
ORIGIN      
        1 tcaaggagca tggccccgct ccgctgccga agatcgccga gagcctgaag ttggagcaga
       61 gcgaagtcgg ttcctccttc ggcctgcttt ccaaaggcgg gttcgtcggt atgaatgaag
      121 caaacaacgc cgaagccaaa gcccgggagc tggatgggat gatcctcttg acccggacag
      181 tgctgaagaa aggcctcgat gggctcctgg atgacgatac cctaacaagt gaagaaagaa
      241 cggcggtctc ccggatcgcc aagaagcgcg gcgcggcgac ctcgccgttc aaggtggtcg
      301 agcgggagga ggtgacctac accctcaccg aggcgggcct gaaagccaag gaggcgatca
      361 tcgccctcaa gatcaccggc gaggagctgg ggctggtaac gtccgagatc ctttccaccg
      421 gatcgtggaa gaacgcgacg ttccgtccct atgccttgaa cgctcccacc agccgcctca
      481 tcgtcggccg gcacaatgcg tacgccgact acctgcagtg ggtgaagaac aagctgatca
      541 gcctcggctt cgaagagttc gacggctcgc tggtggaaag cgagttctgg aacggggatg
      601 ccctcttcat gccgcagttc cactccgccc gggacatcca tgacgtctac tatgtcaaag
      661 aacccaggca cgccaagacg atcgaggagc cgtggctcag tcaggtcgca gcgacccatg
      721 agaacggatg gaagacgaac agccggggtt ggggctacac cttcgaccgc gagttcaccc
      781 gccgccagat ccttcgaagc caggggacgg ttctttccgc ccgccagctt cccaaagcga
      841 aggttcccgg caagtacttc ggcgtcgtcc ggtgcttccg gtacgatcag gtcgatgcga
      901 cccacggagc ggatttctat cagaccgagg gaatcgtcct tggcgaagag gtgaacctga
      961 agaacctgct cggtctcttg aaaatgttcg ccgaggagat cgccggagcg gaagagggtg
     1021 tgcttttggc atttgagcga aggcacaact tcgcctccgc ctggaagggc ctgtcgctac
     1081 cgtcgctaac attggtccgc aatggcgagg tgtgctgggt ttgcgctgat ggccgggcac
     1141 cgattcgagc a
//
LOCUS       DAOPUZ010000438         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_146574_length_1148_cov_3.216834, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000438 DAOPUZ010000000
VERSION     DAOPUZ010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146574_length_1148_cov_3.216834"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKN79_06695"
     CDS             <1..887
                     /locus_tag="PKN79_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNZ95487.1"
                     /translation="AALMGLECDIYMGSVDVRKEAPNVSRMKILGAKVIEVERGTQSL
                     KDAVDAAFEAYLGDPVDQIYCIGSVVGPHPFPMMVRDFQSIVGIEAKEQIQEMIGRLP
                     EAIVACVGGGSNAMGIFSAFLDDTDVAIYGVEPAGKGLDTPNHAATITKGSVGILHGF
                     KSLVLQDEQGEALPVYSIASGLDYPGVGPQHSFLHSIKRVIYESITDREAVEAFYGLS
                     RMEGIIPALESSHAVAYAIKLAQTMSPNEVILVNLSGRGDKDIDFVLEHYPLEEYEPE
                     AKNGRDGFKEFLAHIKTK"
     gene            complement(894..>1148)
                     /locus_tag="PKN79_06700"
     CDS             complement(894..>1148)
                     /locus_tag="PKN79_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95488.1"
                     /translation="AVCLWHPDSTLLKSMAVANWILAAATSTLRVVGGSHHVHDVLAG
                     AAIGGGLGFLGPFITDRLQKRDRQAPRVLIGQATGVLVSY"
BASE COUNT          260 a          326 c          351 g          211 t
ORIGIN      
        1 cagcggcgtt gatgggcctg gagtgtgaca tctacatggg gagcgtcgat gtccgcaaag
       61 aagcgccgaa cgtcagccgg atgaagattc tcggtgcgaa ggtcatcgag gttgaaagag
      121 gaactcaatc cctcaaagac gctgttgatg ccgcctttga agcgtatctg ggcgatccgg
      181 tcgatcagat ctactgcatc ggctcggtag tcggccccca cccctttccg atgatggtcc
      241 gcgatttcca atcgatcgtc ggcattgagg cgaaagaaca gattcaagag atgatcgggc
      301 gcctgcccga ggcgatcgtg gcatgtgtcg gcggcgggtc gaatgcgatg ggaatcttca
      361 gcgcgttcct ggacgacacg gatgtggcga tctacggagt cgagccggcg ggaaagggac
      421 tggacacgcc caaccacgct gcgacgatca ccaagggaag cgtgggaatc cttcacggct
      481 tcaaaagtct cgttcttcag gacgaacaag gggaagctct tcccgtctat tcgatcgcca
      541 gcggcctgga ttaccccggc gtcggaccgc agcactcatt cctacactcg atcaagcggg
      601 tgatctatga atcgatcacc gaccgcgaag cggtggaggc gttctacggc ctttcccgaa
      661 tggaaggaat catccccgcc ctggaatcga gccacgccgt ggcgtatgcg atcaagctgg
      721 ctcaaacgat gagtcccaac gaagtcattc tggtaaacct ttcaggccgg ggggacaagg
      781 atatcgactt cgtgctggag cactatccgc tggaagagta tgaaccggaa gcaaagaacg
      841 gccgggacgg attcaaggag ttcctcgccc acatcaagac gaagtagtcc ctgtcaatag
      901 gaaacaagca ccccggtagc ctgaccgatg agcacccggg gtgcttgtct gtcgcgcttt
      961 tgcagtctgt cggtgatgaa aggcccgaga aagccgagac ctcccccgat cgccgctccg
     1021 gccaggacgt catggacatg gtgggagcct ccaacgaccc gaagggtcga ggtggctgcc
     1081 gccaggatcc agttggcgac cgccatcgat ttcaacagcg tcgaatccgg atgccacagg
     1141 cacaccgc
//
LOCUS       DAOPUZ010000439         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_146834_length_1147_cov_2.232601, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000439 DAOPUZ010000000
VERSION     DAOPUZ010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146834_length_1147_cov_2.232601"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..1147
                     /locus_tag="PKN79_06705"
                     /pseudo
     CDS             255..1147
                     /locus_tag="PKN79_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT          293 a          313 c          312 g          229 t
ORIGIN      
        1 ctccgcttct ggagttcctc gtggtgggct tcatttcgca gtttttctcc tggctgtgca
       61 tgttccttct gcccagaagc gagtgggaag ccgtccttaa gatgatcacg atttacttct
      121 tggtaatcta tccatttttc atggcggctc tgggaatcgt tctcctctcc cagatccaac
      181 gaatcacggc gatgcgtgaa atcgcccgca acgaattcat cctgaaaacg atctttgacg
      241 atgctccgat cggcatgagc cgaacggatc tgaaaacggg caaggtgctg cagatcaatg
      301 acgcctacgc ccgtttcctg gggtatgaca aggaggagat cgtcgggcat acatggaagg
      361 aattcaccct ccctgaagaa caaggtgaaa gcgacaaggc ggatttcctg ctggtaagcg
      421 gatcccgcaa ccaagtaacc cttgacaaac ggtaccgaac aaaagatggc cggatcgtca
      481 gcggcaacct caccgtatcc aacctctcga tcgggaaggg gacggaggaa atactcggaa
      541 tgctcgtgga tatcaccgac cggaaggagt atgaagaaag gctgctccgc ctctccaccc
      601 atgactccct caccaacctc tacagtcgaa accattttga gcgaacgatt gaagagttcg
      661 tggttcccaa ggatcaggtt gtggcggtgc tgctggccga agtgaacgga ttgaagatcc
      721 tcaacgaagc gttcggtcag gacgaaggaa ccgcctgctg aagaagatcg ccaagatcct
      781 ccaagcctcg acggaggaga ccgattacct ggcccggatc ggtggagacg atttcgtcgt
      841 gattctcacc ggtcgcactc ggcatgaata ccaggcgatc gccaaggaca tcacggagaa
      901 actctccctc gtccagatgc ccggagggat ggacatgagc gtcagcgtcg tccatgccgt
      961 ggcggatgaa agggatcgga ccctccacaa agcgatcaaa gaagcggaaa aaaccttgaa
     1021 gacccgcaag cttttcgagg aggccccgat catagagaac ctcgtgcaga ccgtcatgaa
     1081 cgcgttgcac gttcgaaata agtcggaaga gcaccacagc cgaagagttt ccttcctcag
     1141 cgaacag
//
LOCUS       DAOPUZ010000440         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_147784_length_1142_cov_3.841766, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000440 DAOPUZ010000000
VERSION     DAOPUZ010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147784_length_1142_cov_3.841766"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PKN79_06710"
     CDS             complement(<1..504)
                     /locus_tag="PKN79_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HNZ95489.1"
                     /translation="MDLIPAIDLIEGKVVRLAQGDYDQKTIYSSNPLEVAKAFEDHGI
                     RRLHVVDLEGAKGGGIVNLETLETLARNTALIIDFGGGIKKEEDLRQAFDAGARYVTV
                     GSIAVTEPALVKEWIQRFGSDRLILGADSKGGIIRTHGWLKESGLDVQSFIESYLHEG
                     LDTVICTD"
     gene            complement(504..>1142)
                     /gene="hisB"
                     /locus_tag="PKN79_06715"
     CDS             complement(504..>1142)
                     /gene="hisB"
                     /locus_tag="PKN79_06715"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNZ95490.1"
                     /translation="VSDRWGEIASFLLDEPHLAPRTAEVTRTTKETSITLSLSLDGGG
                     LGEIHTHIPFFDHMLDQIRRHSGCDLSLHADGDLMIDEHHTVEDVGIALGEAFRSALG
                     EKRGINRYGFEVLTMDEVKAEVALDFSNRPAFTYDVPIAREYIGTFPTEMVEHFFKSF
                     SDSARCNLHMKVGTGNAHHQVEGLFKAFARAVRQAIHRNPYSDELPSTKGVL"
BASE COUNT          223 a          361 c          315 g          243 t
ORIGIN      
        1 gtcggtacag attaccgtat caaggccttc atgaaggtag gactcgatga acgattggac
       61 atcaagtccg ctttctttca gccacccgtg ggtccgaatg attccccctt tgctgtcggc
      121 gccgagaatc aggcggtccg agccgaacct ctgaatccac tccttgacca aagcgggttc
      181 ggtcaccgcg atcgaaccga ccgtcacata gcgcgccccg gcgtcgaatg cctgcctcag
      241 gtcctcttcc ttcttgatcc ccccgccgaa atcgatgatg agagcggtgt tccgggcaag
      301 ggtttcaagc gtttcaaggt tcacgatccc cccgcccttc gccccttcca gatcgacgac
      361 gtgcaggcgc ctgatcccat ggtcttcaaa ggctttggcc acctccaagg ggtttgagga
      421 gtagatcgtc ttttggtcgt agtcgccctg ggcaaggcgg acgaccttcc cttcgatcag
      481 gtcgatcgcc ggaatgagat ccatcacagc acccccttcg tcgacggcag ctcatcactg
      541 tacggattgc ggtggatcgc ctggcggacc gcccgggcga acgccttgaa cagcccctcc
      601 acctggtggt gggcgtttcc cgtacccacc ttcatatgca gattgcaccg ggcgctgtcg
      661 gaaaacgact tgaagaagtg ctcgaccatc tcggtgggga aggttccgat atactcccgg
      721 gcgatcggga cgtcataggt gaacgccgga cggttggaaa aatccaaggc gacttccgct
      781 ttcacctcat ccatcgtcag cacctcgaat ccgtaccggt tgatcccccg cttctccccc
      841 aatgcactgc gaaacgcttc acccaaggcg atgccgacat cctccaccgt atggtgctcg
      901 tcgatcatca gatccccgtc ggcatggagg gaaagatcac acccgctgtg cctcctgatc
      961 tgatcgagca tatggtcgaa gaaggggatg tgggtatgga tctccccgag tcctcctcca
     1021 tccagcgaga ggctgagggt tattgaggtt tccttggtcg tgcgggtgac ctccgccgtt
     1081 ctgggagcca gatgcggctc gtcaagcaga aaggaagcga tctcccccca tcggtcggaa
     1141 ac
//
LOCUS       DAOPUZ010000441         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_148727_length_1138_cov_2.638042, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000441 DAOPUZ010000000
VERSION     DAOPUZ010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148727_length_1138_cov_2.638042"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="glnS"
                     /locus_tag="PKN79_06720"
     CDS             complement(<1..1055)
                     /gene="glnS"
                     /locus_tag="PKN79_06720"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNZ95491.1"
                     /translation="MKAGKHPEGSYVLRAKIDMASPNMNLRDPVLYRIKFATHPRTGD
                     DWCIYPMYDYTHPISDAIEGITHSICTLEFEDHRPAYDWATSIDLIDHTPRQYEFARL
                     NIDYTLMSKRRLLSLVQMGVVDGWDDPRMPTISGIRRRGYSPEAMLDFVGRIGVAKVE
                     GIVEHSLLEFCVREDLNKRAKRLMVVLDPIKVVIDNFPEGKVEYVEALNNPEDPEGGT
                     RLVPFSRKVFIEREDFMEKPPKGYHRLSIGTEIRLIYAYYITAHSVVKDDDGNVLEVH
                     CTYDPESRGGQTADGRRVRGTSHWVSAAEGVSIEVRLYDKLFLAEEPGKETGNFLDDL
                     NPESLIVVEDAKAEPEVL"
BASE COUNT          219 a          316 c          322 g          281 t
ORIGIN      
        1 agaacctccg gttcagcttt tgcatcttcg accacaatca atgattccgg gttcagatcg
       61 tccagaaagt tccccgtttc cttcccgggt tcttcggcaa ggaagagctt gtcgtacagc
      121 cgaacctcga ttgatacccc ttcggcggcg ctgacccagt gactggttcc ccggactctt
      181 cggccatcgg cggtctgccc gccgcgggat tcgggatcgt aggtgcagtg aacttcaagg
      241 acatttccat catcgtcctt tacgacggag tgggcggtga tgtagtacgc atagatcagc
      301 cgaatctcag tgccgatcga gaggcgatga taccctttgg ggggcttttc catgaaatct
      361 tcccgttcga tgaacacttt gcggctaaag ggaacgaggc gggttccacc ttccggatcc
      421 tcaggattgt tgagcgcttc cacatattcg accttccctt cagggaaatt gtcgatgacg
      481 accttgattg gatcgaggac caccatcagc cgtttcgccc gtttgttcaa gtcttcgcgg
      541 acgcagaact ccaacaggga gtgttccacg attccttcaa ccttcgccac tccgattctg
      601 cctacaaaat caagcatcgc ttccggcgag tagcctcggc ggcgaatacc gctgatcgtc
      661 ggcatccggg gatcgtccca gccatcgacg acgcccatct gcaccagact cagcaggcgg
      721 cgcttgctca tcagggtata gtcgatgttc aagcgggcga actcgtattg tcggggggta
      781 tgatcaatga ggtcgatcga tgtcgcccag tcatatgccg ggcgatggtc ttcaaactcg
      841 agggtgcaga tcgaatgggt gatcccttcg atcgcgtcgc tgatcgggtg ggtgtaatcg
      901 tacatcgggt agatgcacca gtcatctccg gttcggggat gggtggcgaa cttgatccga
      961 tagagaaccg gatcgcgaag gttcatgttc ggactggcca tgtcgatctt cgcccgcagc
     1021 acataggagc cttcggggtg cttgcctgct ttcatctctt cctaatgctg aaggttttct
     1081 tcgatcgggc gatcgcgata ggggctgttt tttcccggtt cggtcaaggt gccgcgat
//
LOCUS       DAOPUZ010000442         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_148912_length_1137_cov_3.161738, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000442 DAOPUZ010000000
VERSION     DAOPUZ010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148912_length_1137_cov_3.161738"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PKN79_06725"
     CDS             <1..768
                     /locus_tag="PKN79_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNZ95492.1"
                     /translation="RKRLSDEIWSVVQSKAQESFEQTGSKMVVIQDEQIERGVASSLA
                     SRLVKAYNAPALVLSSSRSGRIKGSMRANDSTIHTRNFLEHFDRQGSYFLYYGGHANA
                     SGFTMESDRLEELMKALHEYVDTIDCPEERVKEYFIDATISEEEMHPELIRLVEFFEP
                     YGEGNRPLVFEIQGAVVEEIGYLNNSQGGDRHVKLTIGYGHYRWPAVYWFAAPLVGKV
                     FDRGSVLDILFRLARNYWMGNETLQLNIVDVKPTKAL"
     gene            complement(755..>1137)
                     /locus_tag="PKN79_06730"
     CDS             complement(755..>1137)
                     /locus_tag="PKN79_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ95493.1"
                     /translation="NTLAPAICGGTLKVLSILQEDHSLRKRTLANADHFRNLLKTAGF
                     DLIDATTAIVAVMVYEEAKAVEFAEKLLNEGIYVIGFSYPVVPKGKARIRVQLSASHT
                     TEDIERAAKAFISVGKTIGIITEL"
BASE COUNT          266 a          288 c          322 g          261 t
ORIGIN      
        1 cgcaagcgcc tcagtgatga gatttggagc gtggtccaaa gcaaggctca agaaagtttc
       61 gagcagaccg gctccaagat ggtcgtcatc caggatgaac agattgagag gggagttgcc
      121 agttcactcg cttcgcgctt ggtgaaagcg tacaatgctc ccgctctggt gctttcctcg
      181 agccgtagcg gacggataaa gggttcgatg cgcgccaatg actcgacgat ccatacccga
      241 aactttctgg agcatttcga tcggcaaggt tcctatttcc tctactatgg cggacatgca
      301 aacgcctcgg gattcacgat ggaatcggac cggcttgaag aattgatgaa ggcgctccac
      361 gaatatgtgg atacgatcga ttgtcccgag gaacgagtga aagagtattt catcgatgca
      421 acgatcagcg aagaggagat gcaccccgag ttgatccggt tggtagaatt cttcgagccg
      481 tacggagaag ggaatcgacc cctcgtattt gaaatccagg gagcggtcgt cgaagagatc
      541 ggctatctga acaacagcca aggcggggac cgccacgtca agttgacgat aggatacggt
      601 cactaccgct ggccggcggt ctattggttc gccgctccgc ttgttggaaa ggtgttcgat
      661 cggggcagtg tgctggacat cctctttcga cttgcccgaa actactggat gggaaatgag
      721 acgctgcagc tgaacatcgt ggatgtgaag ccgactaaag ctctgtgatg atccctatcg
      781 tttttcccac cgagataaat gccttcgcgg ctcgttcgat atcctcggtc gtatgggatg
      841 ccgacagttg aacgcggatg cgtgcttttc ccttgggaac taccggatag gaaaagccga
      901 tcacatagat tccttcattc agcagcttct cggcaaattc aacggctttc gcctcttcat
      961 acaccataac ggcaacaatt gccgtagtcg cgtcgatcag atcgaacccc gccgttttca
     1021 gcaagttcct gaagtgatcg gcgtttgcca gagttcgctt gcgcaaggag tgatcttcct
     1081 ggagaatgga gagcaccttc agcgttcctc cgcagatcgc cggagccaga gtgttgg
//
LOCUS       DAOPUZ010000443         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_149004_length_1137_cov_2.104436, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000443 DAOPUZ010000000
VERSION     DAOPUZ010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149004_length_1137_cov_2.104436"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1122)
                     /locus_tag="PKN79_06735"
                     /pseudo
     CDS             complement(94..1122)
                     /locus_tag="PKN79_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT          217 a          324 c          356 g          240 t
ORIGIN      
        1 ggggtgagcc tcgatccgaa gacgatgctg atttcaaagt aggaaacagg tcgcattctc
       61 cccaagacag ccggcgaaag ccggctgtcg gcgtcaatag aggaagggtg ccgcctgttt
      121 cagggttctt ttcccgatcg catcctccaa gagggcgatg ctttcccttc ggatgatgta
      181 gagaacccga ttcagcgtat caagaacgac cgcatgcacc cccggtttct ccgatggcga
      241 aagcgcatcg cccgattcaa ggaactccgc cttcgccttg gcggtcactt ggaagggggt
      301 gagatcccag agcgtcagat cgagactcgt ctccagctcc tcctcgatgt gatcggggag
      361 gcggtggaag aaatccagcc cggtcagttc ctcgacctcc cgaaccgtca gggcgaactc
      421 gctcacatcc cgttttgaat ccttgttggg aagcacgaac ccgatcgctt tcagcgtcgg
      481 ctcctcgtag tcgaggatta ctttgtagta ctggttgggg acggatactt cattcctgcc
      541 gatcgtcttg tacggcccgt cggtgagcac tggaccggtg accacgtaga cggcgcggtc
      601 cacgttggcg atgttgcgga ccaccgcctc gagcgtcgcc tagatgccgc ggttgaagga
      661 gggttcctga gggctcatgt tggacatgta gaacgagccg ctcatcgcct cctcgctgcg
      721 aaccagatcc gcggcgggaa tgaggtgtcc ccggtcgtag ccgcttccgc ggtaatcgtc
      781 cggcgtcgcc gatccggtgg gaatcgaggg gtcggcccgg aagttgtcct ttcgctccgc
      841 ctcaccgtag agatccgccc gggtcagccg gtaggcgacc caggaagcct gttcatgttc
      901 ttcctcatag cgcagggtgt acccggggtg ggagacgact agtgcacctt cttcatagga
      961 gggaagctcc agatcgggaa tggctgctcc tatcagggac gggtcgtcgg cgggggcgaa
     1021 gaggagaacg agagcccaca ggatgagcgc accgacgaag agcaggagaa tccgtttcag
     1081 gcgatgtttc ttgcgaacgc ctttcttttt tactttggtc atggattcac ggtagcc
//
LOCUS       DAOPUZ010000444         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_149595_length_1134_cov_2.870250, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000444 DAOPUZ010000000
VERSION     DAOPUZ010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149595_length_1134_cov_2.870250"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PKN79_06740"
     CDS             <1..523
                     /locus_tag="PKN79_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNZ95494.1"
                     /translation="ALPFRLEVVIADEETSLDIGQRVVCERYLVVDRIRSPFNLGSIF
                     RLADSFGIKKIYIVEGGAEPTHQRTIKVGRGTVETVEYEVVSEDSLLAGLKNSELPLF
                     ALESGGVDITEFDFPLAGICVIGSEELGVSPALLDLCDKRSGRVSIALGGTKGSLNVT
                     SASAIMLHIWFVR"
     gene            complement(526..>1134)
                     /locus_tag="PKN79_06745"
     CDS             complement(526..>1134)
                     /locus_tag="PKN79_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HNZ95495.1"
                     /translation="APGTEGIVNIEHEAGLSGEIHDKGLLILEGYLRKHYARSFPLSI
                     YAGIAFEQSYAEVDGDSASSSELFALLSAIGELEVRQDIAVTGSVNQMGEIQPVGGIN
                     EKIEGFFHTCRFLGLTGKQGVIIPYQNVSNLILSYEILQAIRDRIFFIYPIRTIEEGM
                     RILTGRPSGVRNSKGNWSAGNFNYDIENRLKRMYQAGLSGRN"
BASE COUNT          220 a          320 c          338 g          256 t
ORIGIN      
        1 tgcccttcca tttcggctcg aagtcgtaat agccgacgaa gagacgagcc tcgacatcgg
       61 gcaacgagtg gtttgcgagc ggtatctggt ggtcgatcgg atccgctctc cgttcaacct
      121 cggttcgatc ttccgcctcg ccgactcctt cggcatcaaa aagatttaca tcgtggaggg
      181 tggcgcggag ccgacccatc agcgaacgat aaaggtcggc agagggacgg tggaaaccgt
      241 cgaatatgag gttgtttcgg aagactcgct gcttgcaggc ttgaagaaca gcgagctgcc
      301 tctctttgcg ctggagagcg ggggagttga tatcaccgag ttcgattttc cccttgcagg
      361 aatctgcgtc atcggcagcg aagagctcgg agtcagtccg gctctcctcg atctgtgcga
      421 caagcgttcg gggcgggttt cgatcgccct gggcgggacg aagggatcct tgaacgtgac
      481 ctccgcaagc gcgatcatgc tgcacatctg gtttgttcgg taggatcagt ttcttcccga
      541 caggcccgcc tgatacatcc gcttcaggcg attctcgata tcgtagttga agtttcccgc
      601 gctccagttg cctttggagt ttctgacccc gcttggccgg ccggtaagaa tccgcatccc
      661 ctcttcgatc gttctgatcg ggtagatgaa gaagatccga tccctgatcg cctgaaggat
      721 ctcgtaggag agaatgaggt tcgagacgtt ctggtagggg atgatcaccc cctgttttcc
      781 ggtgagaccc agaaagcggc aggtgtggaa gaacccctca atcttttcat tgatcccccc
      841 caccggctgg atctccccca tctggttgac cgaaccggtg acggcgatgt cctggcggac
      901 ctcaagctcc ccgatcgccg aaagcagggc gaaaagttcg ctggaggaag cactgtcccc
      961 gtcgacctcc gcataggact gttcgaaggc gatcccggcg tagatcgaaa gagggaagga
     1021 gcgggcgtag tgcttgcgca gatatccctc cagaatcagg aggcctttgt cgtggatctc
     1081 gccgctcagc cccgcttcgt gttcgatatt gacgattcct tccgttcccg gggc
//
LOCUS       DAOPUZ010000445         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_150191_length_1131_cov_3.840149, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000445 DAOPUZ010000000
VERSION     DAOPUZ010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150191_length_1131_cov_3.840149"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1131)
                     /locus_tag="PKN79_06750"
     CDS             complement(<1..>1131)
                     /locus_tag="PKN79_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ95496.1"
                     /translation="HDFNYGYKRLVEAAGFAEEYSNLRVLSANVVDEDGYLLFQPYQV
                     YDFNGFTVGVVGLTTPDTKTKSHPKNTAGLTILNEEIFAIGQQAIDIARTYVDYVIVL
                     GHIGLDPDGASGLTSDMIVSKIKGIDLFVDGHSHDALEKGLKIGDSYIVSAGEYLENV
                     GVVEINVRGGKVVGTTPMLIPAEDVLNPEESDLARAYGIVDVPNHPKVDEYVTYMQAQ
                     LSDKLNQVIATIPVDLNGERAHVRTRPTNLSKIITAAMTEQSGADFTITNGGGIRASL
                     KAGPVTIGDVINVLPFTNILTVVEATGAEVYAALEHGYRMLPETNGGFAQTDLSVVYN
                     RFAEPGKRIVKVLLNGKAIDKNKTYRVATNDFLAAGGDGYTM"
BASE COUNT          220 a          318 c          324 g          269 t
ORIGIN      
        1 aacatcgtgt atccgtctcc gccggcggcc aggaagtcat tggtcgctac gcggtaggtc
       61 ttgttcttgt cgatcgcctt gccgttgagc aggactttga cgatgcgctt gccgggctcg
      121 gcaaatcggt tgtacacgac gctcaaatcc gtctgggcga atccgccgtt cgtttccggc
      181 agcattcgat acccatgctc aagagccgca tatacctcgg cgccggtcgc ttcgaccacc
      241 gtaaggatgt tcgtaaacgg cagcacgttg atcacatcac cgatcgttac cggaccggcc
      301 ttcagggaag ctcggattcc accaccgttg gtgatcgtga agtcggctcc gctctgttcg
      361 gtcatcgcgg cggtgatgat cttcgaaaga ttcgtcgggc gggttcgaac atgggcccgc
      421 tcaccgttca agtcaaccgg aatcgtagcg atgacctgat tgagcttgtc gctgagttga
      481 gcctgcatat aggttacata ctcatccact ttgggatggt tcggcacgtc cacgattcca
      541 taggcccggg cgagatcgct ctcttcaggg ttgaggacat cttcggcggg aatcagcatc
      601 ggtgtcgttc cgaccacttt gccgcctcgg acgttgatct cgacgactcc gacgttctcc
      661 aggtattcac cggcggagac gatgtagctg tcgccgatct taagaccctt ctccagcgca
      721 tcgtgggagt gcccgtcgac gaacaggtcg attcccttga tcttactgac gatcatgtca
      781 ctggtcagtc cgctcgcccc gtccggatca aggccgatat ggccaagaac gatgacgtag
      841 tccacgtagg tgcgggcgat atcgatcgcc tgctgaccga tggcgaagat ctcttcgttg
      901 aggatggtga gacccgcagt gttcttcgga tggctcttcg tcttcgtatc gggggtcgtc
      961 agaccgacga ctccgacggt gaagccgttg aaatcgtaca cctggtacgg ttggaacaag
     1021 agatagccgt cttcatcaac aacgttggct gaaagaactc ggagattaga atactcttcg
     1081 gcgaatccag cagcttcgac caggcgcttg tatccgtagt tgaagtcgtg g
//
LOCUS       DAOPUZ010000446         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_150422_length_1130_cov_3.556279, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000446 DAOPUZ010000000
VERSION     DAOPUZ010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150422_length_1130_cov_3.556279"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKN79_06755"
     CDS             complement(<1..286)
                     /locus_tag="PKN79_06755"
                     /inference="COORDINATES: protein motif:HMM:NF016000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNZ95497.1"
                     /translation="MIIDSLSNLGRYIPTVPALRLVREILEEDLLSKDLGSYKTSDPN
                     LRYIIMTYRTEKEEADLYEVHRKETDVQILLQGTEVMEIGSRESFRETVAY"
     gene            381..815
                     /locus_tag="PKN79_06760"
     CDS             381..815
                     /locus_tag="PKN79_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNZ95498.1"
                     /translation="MSDNHSYTTNIEWTRNRKGALSSPGLPTMEVATPPNFPGGHPGI
                     WSPEHLFVAAAEVCLMTTFLAIAEKSKLDFLAYRSSATGTLGKLDRGMFVEKIVIRPN
                     VVISDENQREKALTVLEKAKKHCLISKSMVTEVTMEPEVVVR"
     gene            820..>1130
                     /locus_tag="PKN79_06765"
     CDS             820..>1130
                     /locus_tag="PKN79_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ95499.1"
                     /translation="MNKKRILVVLVSLVIAVGFLGARDGSLAIIHMNDTHSFLLAGRD
                     EHTGREFGGAARWKTIIDELRGDRKDVLVLHAGDFLTGSGGDYLSFERIDYDRFPLYG
                     WR"
BASE COUNT          259 a          276 c          327 g          268 t
ORIGIN      
        1 catacgccac cgtttctctg aagctttccc tcgatccgat ctccatcact tccgtgccct
       61 gaagaagaat ctgcacatcg gtttccttcc tgtggacttc atagagatcc gcctcttcct
      121 tttccgtccg ataggtcatg atgatgtagc gaagattcgg atctgatgtt ttgtatgatc
      181 cgagatcctt ggagagaagg tcttcttcga gaatctcccg gacaaggcgc aatgccggaa
      241 cggtagggat atagcggccc agattggata gcgaatcgat gatcatggcg gctcctttgg
      301 tccaccatac cgcctaagag ggttcttgcg ctagcctaat atggcaagtg tgagtattgt
      361 tgtggcaagg agtgatgaat atgagtgaca atcatagcta tacgacgaac atagagtgga
      421 cccgaaacag gaagggagcg ttgtcttcac cggggcttcc cacgatggag gtggcgaccc
      481 cgccgaactt ccccggcgga catccgggaa tctggtcgcc ggagcacctc ttcgtggctg
      541 cggccgaggt gtgtctgatg acaacgtttc tggcgatcgc cgaaaaatcg aagctggatt
      601 ttcttgccta tcgatcgagc gcaacgggaa cgctgggaaa gcttgaccgg ggaatgttcg
      661 tcgagaagat cgtcatcagg ccaaatgtgg tgatcagcga tgaaaaccaa cgggagaaag
      721 ccctcacggt gctcgaaaag gcgaaaaaac actgtcttat ctcaaaatcg atggtcacgg
      781 aggtaacgat ggagccggag gtcgtggtac gctaagggca tgaataaaaa acgtattctt
      841 gttgttcttg taagtttggt tatcgccgtc ggatttctgg gcgccagaga cggctcgctt
      901 gcgatcatcc acatgaatga cactcactcg ttcctgcttg ccgggaggga tgagcatacg
      961 ggtcgggaat tcggcggagc ggctcgctgg aaaacgatca tcgacgagct tcggggtgac
     1021 cggaaggatg ttcttgtcct ccatgccggt gactttctta ccggaagcgg cggcgattac
     1081 ctgagctttg aacggatcga ttatgaccgc ttccccctct atggatggcg
//
LOCUS       DAOPUZ010000447         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_151260_length_1126_cov_4.156863, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000447 DAOPUZ010000000
VERSION     DAOPUZ010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151260_length_1126_cov_4.156863"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PKN79_06770"
     CDS             <1..873
                     /locus_tag="PKN79_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNZ95500.1"
                     /translation="ALDQHLAGSPDFLLGAAAQLGIFAALIGALAISFIPGINFTLHD
                     AAAIGIIRGADGPTAIYVTSRLAPNLLGPIAVAAYSYMALVPIIQPPIMRALTTEEER
                     KIKMKQLRPVSRLEKILFPLSVLTMCAILLPSATPLVGALMFGNLAREVGVINRLSDT
                     MQNALMNIVTIFLGLAVGSKMEARLFLNLNTLGILFLGMVAFSLGTAGGVLIAKLMNK
                     LDKKHPINPLIGSAGVSAVPMAARVSSKVGQEADPQNFLLMHAMGPNVSGVIGSAVAA
                     GVLLAVVPFMEVLV"
     gene            complement(936..>1126)
                     /locus_tag="PKN79_06775"
     CDS             complement(936..>1126)
                     /locus_tag="PKN79_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ95501.1"
                     /translation="GRWGGIEVKLGENEVDKASANLLKLATISTRKPAFLMIVTNTRM
                     AYQRFDGVYVIPLGCLKP"
BASE COUNT          224 a          358 c          300 g          244 t
ORIGIN      
        1 gcgctcgacc agcacctggc gggctccccc gacttcctcc tcggcgccgc cgcccagctg
       61 ggaatcttcg ccgccctcat cggggcgctg gcgatcagct tcattccggg aatcaacttc
      121 accctgcacg acgcagcggc gatcggcatc atccgaggag cggacggacc gacggcgatc
      181 tacgtcacca gccgcctggc tccgaacctt ctcggaccga tcgccgtggc cgcgtacagc
      241 tacatggccc ttgttccgat catccagccc ccgatcatgc gggcgctgac cacggaagag
      301 gaacggaaga tcaagatgaa gcagctgcga cccgtctcca ggctggagaa gatcctcttc
      361 cccttaagcg tgttgacgat gtgcgcgatt ctgcttccct cggcgacccc gctcgtcggg
      421 gcgctgatgt tcggcaacct cgcccgggaa gtcggggtga tcaatcgcct cagcgacacg
      481 atgcagaacg cgttgatgaa catcgtaacg atcttcttgg gccttgcggt cggatcgaag
      541 atggaagccc ggctcttcct taacctcaac accctcggca tcctgttcct cggaatggtc
      601 gccttcagcc tcggcaccgc cggcggcgtg ctgatcgcaa aattgatgaa caagctcgac
      661 aaaaagcatc cgatcaaccc cctgatcgga agcgccggag tctccgccgt tccgatggcc
      721 gctcgtgtat catcgaaggt cggtcaggaa gcggatcccc agaacttcct gctgatgcac
      781 gcgatgggac cgaacgtctc gggagtcatc ggatcggccg tagcggccgg cgtgcttctg
      841 gccgtcgttc ccttcatgga agtattggtc tgatcaacag caaccagtca actcagggcc
      901 cttcggggcc cttggttgtt tacgagacag gttcatcaag gttttaagca tccaagcggt
      961 attacataca caccgtcaaa tctttgatag gccattcgag tatttgtcac tatcatgaga
     1021 aaagccggtt tacgagtgga tatggttgct aatttcagta aattggcact tgccttatcg
     1081 acttcatttt ctcccaactt tacctctatt ccgccccacc ttccat
//
LOCUS       DAOPUZ010000448         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_151714_length_1124_cov_3.917680, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000448 DAOPUZ010000000
VERSION     DAOPUZ010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151714_length_1124_cov_3.917680"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKN79_06780"
     CDS             complement(<1..778)
                     /locus_tag="PKN79_06780"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNZ95502.1"
                     /translation="MYDVIVVGGGVAGLFLAANLPDRKVLILDHAKSLGKKLLITGGG
                     RANITNDLSAKEMLAHFPTKAMRNFLAPSLLSLTPSDLMNWFENRGVGLTVRADGHVF
                     PSSLDARTIRNALLSHIHTDIRTACTVAAIINEEESFTIHSSEGSFVTRSVCLATGGM
                     SYPSTGSDGSGYALAKALGHTIVPPKPALTPIVTCEQTAHLSGISLSNVSTTHRQSTT
                     TGDLLFTRDGLSGPVILNLSRAVSKGDPVTIAVLDSSLLEE"
     gene            complement(781..>1124)
                     /locus_tag="PKN79_06785"
     CDS             complement(781..>1124)
                     /locus_tag="PKN79_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNZ95503.1"
                     /translation="IDVYNNGDMWRDFTYIDDVINALEHIIPRVPIDPVIYNIGNNNP
                     IRLLRFIEALEEVIGKKANKNFLPMQDGDVYQTYADISDLQRDFGITINTPIEEGLAS
                     FVAWYRSYYNV"
BASE COUNT          257 a          265 c          336 g          266 t
ORIGIN      
        1 tctcttcaag gagagaggaa tcaagaacgg caattgtcac cggatcgcct ttactcaccg
       61 ctcgtgaaag attgaggatg accggtccgc tgagcccgtc tcgggtgaag agaagatcgc
      121 cggttgtagt ggattgtcta tgggtagtgg agacgttgga cagcgaaatg ccgctgaggt
      181 gagctgtctg ttcacaggtg acaatcggag tgagggcggg ttttggcggg acgatcgtgt
      241 gaccgagcgc cttcgccaag gcgtatccgc tgccatcgct accggttgat gggtagctca
      301 ttcctccggt ggccaggcaa acggaccggg tgacaaacga tccctcggat gagtggatcg
      361 taaacgattc ttcttcgttg atgatcgctg caaccgtgca agcggtgcga atatccgtat
      421 ggatgtggga aagaagagca ttgcgtatcg ttcgggcatc gagggaggag ggaaacacat
      481 gtccatccgc tctgacggtg agcccgactc ctcgattctc gaaccaattc atcagatccg
      541 aaggagtgag actcagcagg gaaggggcga gaaagttccg catcgctttc gtcggaaagt
      601 gagcaagcat ttcttttgcc gagaggtcat tggtgatgtt ggccctccct cctccggtga
      661 tcagaagctt tttgcccagc gatttggcat ggtcaagaat cagtaccttt cgatcgggga
      721 gatttgcagc gaggaagagg ccggcaacgc cccctccaac cacgatcacg tcatacattt
      781 ttaaacgttg taataagaac gataccacgc aacgaaggaa gcaagtccct cttcgatcgg
      841 ggtgttgatg gtgatgccga aatcccgctg gagatcggag atgtcggcat acgtttggta
      901 gacatccccg tcctgcatcg gcaggaagtt tttgtttgcc ttcttgccga taacctcttc
      961 aagcgcttcg atgaagcgca agaggcggat cgggttgttg tttccgatat tgtagatgac
     1021 cggatcgatt ggaacacgtg ggatgatatg ttccagagcg ttgattacat catcgatgta
     1081 ggtaaagtcc cgccacatgt caccgttgtt gtacacgtcg atcg
//
LOCUS       DAOPUZ010000449         1121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_152393_length_1121_cov_2.947467, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000449 DAOPUZ010000000
VERSION     DAOPUZ010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152393_length_1121_cov_2.947467"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKN79_06790"
     CDS             <1..319
                     /locus_tag="PKN79_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95504.1"
                     /translation="WISLFRELAHILLRHVGPRIVSSKDAISEAESLGFELLLPSDRW
                     KTFLARKRFDEKAILTFAQEEKIHPSLVANRLGDSKKELAHLTVAYELLRSDVNKPEH
                     PAK"
     gene            complement(371..817)
                     /locus_tag="PKN79_06795"
     CDS             complement(371..817)
                     /locus_tag="PKN79_06795"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNZ95505.1"
                     /translation="MKRLPYHGAGILAWWKDAHGKTEVLLGLRKYNPQANMWSIPGGE
                     WDERKDGFEEGTTPDYRATAIRETWEEIRLSIDDGDDLALLWRTHIPFYHFRVFAYPM
                     PEKRNLVCHEEFSRLRWFPVDDLPNNTVSLVRLQVSALRKDSGGGA"
     gene            complement(864..1079)
                     /locus_tag="PKN79_06800"
     CDS             complement(864..1079)
                     /locus_tag="PKN79_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95506.1"
                     /translation="MERVDIVAFAFSPGGVKKGCPLDRLDGRKGYITIDDALARNWRV
                     FDEDTDELLETYRSSAEVVAGGWKVST"
BASE COUNT          261 a          329 c          263 g          268 t
ORIGIN      
        1 ctggatctct ctgttccggg aactggctca tattctcctc cgacatgtgg gaccgcgcat
       61 agtatcctca aaagatgcca tatccgaggc tgagtccctt ggctttgaac tgttgcttcc
      121 ttccgaccga tggaagacct tccttgcccg caagcgattt gacgaaaaag cgattctcac
      181 cttcgcccaa gaagaaaaga ttcacccttc gctcgtcgcc aatcgcctcg gggattcgaa
      241 gaaggagctt gcccatctca ctgttgcgta tgagctgctt cgaagcgatg tgaacaagcc
      301 ggagcatccg gcaaaataga aatcctgaaa aatccgaaga gcgaggcggg gctatcatca
      361 ttacggctca tcaagctcca ccgccggaat cctttctcaa agcggaaacc tgcaaccgca
      421 ctaacgagac ggtattgttc ggcagatcat cgacagggaa ccacctcagc cgggaaaact
      481 cctcatgaca aacaagattc cgcttctcag gcatgggata tgcgaagact ctgaagtggt
      541 agaaagggat atgggttcgc cacagaagag caaggtcatc accatcgtcg attgacagcc
      601 gtatctcttc ccatgtctcc cgaatggcgg tcgctcgata gtcgggcgtc gttccttctt
      661 caaacccgtc cttccgctca tcccactcgc cgccggggat cgaccacatg ttcgcttgag
      721 gattgtactt tctcaatcca agaagaacct ccgtcttccc gtgagcgtct ttccaccatg
      781 caaggatgcc ggccccatga taggggagcc gtttcattgc tcttccaaga tgtttgatta
      841 ctgaatcgtt gaatgacatg attctatgta cttaccttcc agccaccagc gacgacttcg
      901 gcgcttgaac ggtacgtttc gagcagttca tcggtgtctt cgtcgaacac ccgccaattt
      961 ctggcaagcg catcatcgat cgtgatatac ccttttctgc catcgagacg gtccagagga
     1021 cacccctttt taaccccacc ggggctgaag gcgaaggcga cgatatcgac ccgctccaat
     1081 acatcaatgg ttcgcttctt ctgttccgga ctccacgttt t
//
LOCUS       DAOPUZ010000450         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_153941_length_1114_cov_2.818697, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000450 DAOPUZ010000000
VERSION     DAOPUZ010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153941_length_1114_cov_2.818697"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PKN79_06805"
     CDS             complement(<1..947)
                     /locus_tag="PKN79_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95507.1"
                     /translation="MNIITRSIEPMHFDLLHQTITDPYSPETYKNRINRAVAKAEEHG
                     FSHLVIYADREHFANLTYFTGFDPRFEEALLILAKGMEPILLVGNEGHSYCTIIPYQL
                     DVVLYQSFSLMGQERVPTTKNVLTESYEKAGITGASRIGLIGWKYFRDVEGSDPDYMT
                     DVPHYLVSSLQAIVGKERVLNATSLLINANDGLRTTVDVDELAVLEIAATKASRGVQN
                     MFLNLEEGMSEIEASYYLQFDGNPLVAHPNVNFTPKGVLQGTASPSRHPLTYGSSMNV
                     GFGYRGSMCARTGVYVYDRTDFDKYYPTSWEAAYVPYFK"
BASE COUNT          258 a          276 c          304 g          276 t
ORIGIN      
        1 atcttgaagt acggcacata cgcagcctcc cagctggttg gatagtattt gtcgaaatcc
       61 gtccggtcgt acacatacac tccggttcgg gcgcacatcg aaccgcggta gccgaagccc
      121 acgttcatcg atgaaccgta ggtcagcggg tggcgggagg gactggcggt tccctgaagc
      181 actcccttcg gagtgaagtt cacgttgggg tgggcgacca aagggtttcc gtcaaattgg
      241 agatagtaag atgcttcaat ttcactcatt ccctcctcga ggttcaagaa catgttctgg
      301 actcccctcg aggctttggt tgctgcgatt tcaagaacgg ccagctcatc cacatccacg
      361 gtggtgcgaa gtccgtcatt ggcattgatc agaaggctcg tcgcattgag gacccgttcc
      421 ttgccgacga tcgcttggag ggatgaaaca agatagtgcg gaacatcggt catgtaatcg
      481 gggtccgagc cttcgacgtc cctgaaatac ttccaaccga tgaggccgat tcggcttgca
      541 ccggtgatgc ccgctttctc ataggactcg gtcagaacgt tcttcgtggt cggaacccgc
      601 tcctgaccca tgagggagaa cgattgatac agcacgacat ccaactgata ggggatgatc
      661 gtgcaatagc tgtgcccctc gttgcccacc aacagaatag gttccattcc ttttgcaaga
      721 atgaggagcg cttcctcaaa gcgcggatcg aagccggtga aataggtgag gtttgcaaag
      781 tgctcgcgat cggcatagat gacgagatgg gaaaaaccat gctcttccgc ttttgctact
      841 gctcgattga tccgattttt gtacgtttcc gggctgtacg ggtcggtaat tgtttgatgc
      901 aacagatcaa agtgcatcgg ttcaatcgat ctggtgataa tgttcatgct tgctccttag
      961 aaatacagtt cgtagtgtcc accccctatc atcggcccgg gagggggaga agtcaacaag
     1021 aagttgtggt agctaaagaa gatatgaacc aagagacgaa ggaagtcggg attgtattcg
     1081 tttcttaagc ccccttgacg ttcttatctt cttt
//
LOCUS       DAOPUZ010000451         1110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_154855_length_1110_cov_2.426540, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000451 DAOPUZ010000000
VERSION     DAOPUZ010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154855_length_1110_cov_2.426540"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKN79_06810"
     CDS             <1..464
                     /locus_tag="PKN79_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95508.1"
                     /translation="FAPLGGFVQAGRSDRVVLTRKDGPLAELLVEGNLLNAEAIASLN
                     MDLLAALVGEAVNLDGSALLVDLLSGITPKKSLQRLERTWYTLGGIPDGYWVCENPSQ
                     SSFWIHFGEEVPLLLDHGVTRWMNKERSLILTLVSDNTTKMVFSALGDAPR"
     gene            598..966
                     /locus_tag="PKN79_06815"
     CDS             598..966
                     /locus_tag="PKN79_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95509.1"
                     /translation="MFGAYIRDDLRYEGFFFHASEEPRTNDESTRIEIGEIIVIVNRT
                     SAGRISIRTTDLSDGRISHSYTICTSSGLVCAGKDTFHYQQNEDSPHIEIGTLWAILP
                     VSYIALEGFHSSIMLELVLH"
BASE COUNT          229 a          315 c          294 g          272 t
ORIGIN      
        1 gcttcgctcc gctgggtgga tttgttcagg ccggaaggag cgaccgggtc gtccttaccc
       61 ggaaagacgg tcctctggca gagcttttag ttgaaggaaa tctcctcaac gccgaagcga
      121 tcgcttcgct gaacatggat cttcttgccg cgttggtggg cgaggcggtc aatctcgatg
      181 gttccgccct gttggtggat ctgctcagcg gtattactcc caagaagagc cttcagcggc
      241 ttgaacggac gtggtacacc ctcggcggca ttcccgatgg gtactgggta tgcgaaaacc
      301 cctctcaaag ctcattttgg atccattttg gcgaagaagt tcctcttctc ctcgatcacg
      361 gtgtcacccg ctggatgaac aaggaacgct ccctcatcct gacgctcgtt tcggacaaca
      421 ccacgaagat ggtattttcc gccctcggcg atgcccctag gtagtgatcg gggagtcaaa
      481 gtatttcacc atttgctcat ccgtgtcata ctatgagcaa tgacaacacg gcggatacat
      541 cctccctctc gtcatcgact tgcagcaatg gtggtggtgc ttttggctgg aggtgtgttg
      601 tttggcgcct atatccggga tgatcttcgc tatgaagggt tcttcttcca cgcttccgaa
      661 gaacctcgga cgaatgacga atccactcgt atcgaaatcg gagagatcat cgtgatcgtc
      721 aatcgaacca gcgccgggcg aatcagcatc cggacgacgg atctgagcga tggccggatc
      781 tcccactcct atacgatctg cacgtcaagc ggtctggtgt gcgccggaaa ggatacgttc
      841 cactatcagc aaaacgagga cagcccccac atagagatcg gaaccctctg ggcgatcctt
      901 cccgtttctt atatcgccct tgaagggttc cattcctcca tcatgttgga actcgttctt
      961 cactagcgtt atttcaccac gatattcacc agcttgtcgg ggacgacgat ctccttgacg
     1021 atcgtctttc cttccagcca ggatcggatc ttttcatcat ctcggccgca aggttgacct
     1081 gccgaaaggc ggccacggca cgaagggcgg
//
LOCUS       DAOPUZ010000452         1110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_154893_length_1110_cov_2.152607, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000452 DAOPUZ010000000
VERSION     DAOPUZ010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154893_length_1110_cov_2.152607"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1110)
                     /locus_tag="PKN79_06820"
     CDS             complement(<1..>1110)
                     /locus_tag="PKN79_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNZ95510.1"
                     /translation="VADEIRTKIREEYQRLRTAFEEFLVYYQNLVRGGTRTYEIPEKI
                     AEFTQMWLKIFEEGEEEAILKALVGNLGYFDKIRSNETNESLVSIRESREKYLDIRKR
                     LAVCAAFFLNEENLEPVVEFVAKFLEGHRIQKRAASIVTFADISNLAIKSLTDDKELR
                     AFYKQAYRYIMIDEFQDNNEEQKRLLYLLAEKEGSEADGIPLVKDLAKDKLFFVGDEK
                     QSIYRFRGSDVSVFKALAKELQGIGGMSVKLPKNYRSEPDLISWFNEIFPSIMKNESE
                     PFEADFEPLEFREPKEGIDPKITLAIKPYVKNPEPAGEAMAEPSLAEAYHLAKKIRHI
                     LTSDEYLIPTKNGSPRKPEPNEIAILLRSTSNQYHL"
BASE COUNT          229 a          297 c          285 g          299 t
ORIGIN      
        1 aaggtgatac tggttcgaag tagagcgaag aagaatcgcg atctcgttcg gctcgggctt
       61 tcgaggagat ccattttttg tgggaatgag gtattcgtcg cttgtgagaa tgtggcggat
      121 ttttttagcc agatgatacg cctcggcgag ggagggttcc gccatcgctt ccccggccgg
      181 ttcaggattc ttcacatacg gcttgatcgc caaggtgatc ttcggatcga ttccttcctt
      241 tggttcccgg aactccaaag gctcgaagtc cgcctcgaag ggttcgctct cgtttttcat
      301 aatcgacgga aagatctcat tgaaccagga gatcaggtcc ggttcgctcc ggtagttctt
      361 gggcagcttg acactcatcc ccccgatccc ctgaagctcc ttcgccagag ccttgaacac
      421 ggagacatcg ctgccccgga agcggtagat cgactgcttc tcgtctccga cgaagaagag
      481 cttgtccttg gcgagatcct tgacgagcgg gattccatcc gcttcggatc cctctttttc
      541 cgcaaggaga tagagcaacc gtttttgctc ctcgttgttg tcctggaact cgtcgatcat
      601 gatgtagcga tatgcctgct tgtagaaggc ccgaagttcc ttgtcgtcgg tgagcgattt
      661 gatcgcaagg ttggagatgt ccgcaaaagt tacgattgag gcggcccgct tttggatccg
      721 gtgtccttca aggaacttgg cgacgaactc gacgacgggc tcaaggtttt cctcattgag
      781 gaaaaaggcg gcacagacgg caagcctctt tcggatgtca agatacttct cccgggactc
      841 acgaatcgac acaagactct cattcgtttc attgctcctg attttatcga agtatccaag
      901 attcccgaca agtgccttca ggatcgcttc ctcttccccc tcctcgaaga tcttcagcca
      961 catttgggtg aactcggcga tcttctcagg gatttcgtat gttctcgttc ctcctcgcac
     1021 aagattctga taatagacca gaaactcttc aaaggctgta cgcagccgct gatactcttc
     1081 acgtatcttt gttcggatct catcggcgac
//
LOCUS       DAOPUZ010000453         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_155024_length_1109_cov_3.350095, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000453 DAOPUZ010000000
VERSION     DAOPUZ010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155024_length_1109_cov_3.350095"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PKN79_06825"
     CDS             complement(<1..1006)
                     /locus_tag="PKN79_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HNZ95511.1"
                     /translation="MTEEKKKPKITLIKQVVAPNEPAEETKSTPDKEGEQKPKTTEKR
                     RVVVVKKKVVKAKPAPKPVPSKSEPRKKQDEKSAAPPAARTIVRKEPVVSERLGSSPL
                     HTGPKVIRPTNLPPVPNQGLTINDTPQRVQRPPQPQQGRRVYGTVGGRPARQQEGSRA
                     PRQGGGQRSSQRPPYQGGGQRQDGYVQRPPQGGGFTPRQGGRPGGFRPSPMGEELPPQ
                     DHQRPQKKTFTKRKDQTYKRRDAEEEREFQIQRRKEQAAAKLAAVPKSIDMMEVITVA
                     DLAKKMNLKAAEIIAMLFKMGMMVTINQQIDFETAQIICREYNCDVRLVSLYDETIIA
                     S"
BASE COUNT          171 a          315 c          321 g          302 t
ORIGIN      
        1 cgctggcgat gatcgtctcg tcgtagaggg agaccaatcg gacatcacag ttgtattctc
       61 tgcagatgat ctgtgcggtt tcaaagtcga tctgctggtt gatcgttacc atcattccca
      121 tcttgaagag catagcgatg atttccgcgg ctttcaggtt catcttcttg gcaagatccg
      181 caacggtgat cacctccatc atatcgatgg atttgggaac ggctgccagc ttggcggcgg
      241 cttgttcctt ccgccgctga atctggaact cgcgctcttc ttcagcatcg cggcgcttgt
      301 aggtctgatc ctttcgtttt gtgaaggttt tcttctgcgg gcgctgatga tcctgtggcg
      361 ggagctcctc tcccatcggc gaaggtctga acccgcctgg ccggccacct tggcgcggag
      421 tgaaaccgcc accctgaggc ggtcgctgga catacccatc ttggcgctgt ccgccacctt
      481 ggtagggagg gcgttgagaa gatcgttgtc caccgccttg ccggggcgct cttgatccct
      541 cctgctgtcg agccggtcga cctccgacgg ttccatacac ccgtcgaccc tgctgtggct
      601 ggggcggacg ttgaacccgc tgaggagtgt cattgatcgt aaggccttgg ttgggtaccg
      661 gcggaaggtt cgtcggacga atcactttcg gaccggtatg gagcggcgag gaacccagcc
      721 gctcggagac gacgggctct ttgcggacga tggtgcgagc agccggagga gccgcgctct
      781 tctcatcctg ctttttccgc ggttcgctct tgcttggcac cggcttggga gcgggcttcg
      841 ccttcactac ttttttcttc accaccacga ctcggcgttt ttcggtggtc ttgggttttt
      901 gttcgccttc cttatccggg gtgcttttcg tctcctcagc gggttcgttg ggggcgacta
      961 cttgcttgat cagggtgatt ttaggctttt tcttctcttc cgtcattctt tcctctatga
     1021 gttactcttc ctcaaacgcc aaaccggtgc cgcagtgggg gcatatatcc atatcctcag
     1081 tgatgatgcc accacaggaa ggacaaatg
//
LOCUS       DAOPUZ010000454         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_155741_length_1106_cov_2.358706, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000454 DAOPUZ010000000
VERSION     DAOPUZ010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155741_length_1106_cov_2.358706"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..>1106)
                     /locus_tag="PKN79_06830"
     CDS             complement(98..>1106)
                     /locus_tag="PKN79_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNZ95512.1"
                     /translation="SAKETLAHGLLVAERSGFTAVIDMPNTDPPLTTKARIIKRIEAA
                     NRTKRNVSYHLWAGVTGDPAQIDEVVKSCEELFPSVVGLKLFAGHSTGNMGLVEEEIQ
                     GRVYRHLARRNYTGAVALHCEKEALLRPELYDNADLSTQSLARPPEAEVASIRDQLSL
                     SKEAGFAGHLHIAHLSTIEGLLLVEEAKREGRRISCGVTPHHLLLNRDMAKDRRLYAK
                     MNPPLRDEETRKKLFEALLEGRIDWIESDHAPHTIADKETGASGIPGFSGMLLLLKVL
                     YEHGLSRGMMQSLFGGNVQGTLKLPPREIVLPSYEALPPLIAAASGEYPYDSFRGLFQ
                     S"
BASE COUNT          215 a          313 c          326 g          252 t
ORIGIN      
        1 aagggggtga accgtgaagt catcgcgaaa ggagcggagc tgctcggtct tgaactcaat
       61 gacatcatcg cccggacgat cgaagctctt gcggagatca gctctgaaat aggcctctga
      121 agctgtcgta ggggtattcc ccggaggccg ctgcgatcag agggggcagt gcctcatagg
      181 agggcaggac gatctctcgg ggaggaagct tcagcgttcc ttggacgttg cccccaaaga
      241 ggctttgcat catgcctctt gagagaccat gttcatacaa caccttcaag agaaggagca
      301 tcccgctgaa cccgggaatg ccgcttgccc ccgtttcctt gtcggcgatc gtatgggggg
      361 catgatcgct ctcaatccag tcgatccgcc cttcaagcag ggcctcaaag agctttttac
      421 gtgtttcctc atcccgcagc ggcggattca tcttcgcata gagccttcga tctttcgcca
      481 tgtcccggtt caataggaga tgatgaggag ttacgccgca gctgattctc cttccctccc
      541 tcttggcttc ttccacaagc aaaagccctt cgatcgtgga caggtgggcg atgtgcaggt
      601 gaccggcgaa gcccgcctcc ttgctgaggg agagctgatc ccggatcgaa gcgacctccg
      661 cctcgggagg ccgggcaagg gattgggttg aaagatcggc attgtcatac agctcggggc
      721 ggagcaacgc ttccttttca cagtggagcg caacggcacc ggtgtagttt cgcctcgcca
      781 gatggcggta gacccgccct tggatctcct cttcgacaag gcccatgttg ccggtggagt
      841 gtccggcgaa gagctttaat ccgactacgg aaggaaagag ctcttcgcag cttttgacaa
      901 cctcgtcgat ctgagcgggg tcgcctgtca cccctgccca gagatgatag gaaacgttcc
      961 tctttgtcct gtttgcagct tcgatccgct tgatgatccg cgcttttgtc gtcaacggcg
     1021 gatcggtgtt gggcatatcg atgactgcgg tgaacccgct tctttcagcc acgagaagcc
     1081 cgtgggcaag cgtttccttt gcgctg
//
LOCUS       DAOPUZ010000455         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_156154_length_1104_cov_2.703527, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000455 DAOPUZ010000000
VERSION     DAOPUZ010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156154_length_1104_cov_2.703527"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKN79_06835"
     CDS             complement(<1..324)
                     /locus_tag="PKN79_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S1"
                     /protein_id="HNZ95513.1"
                     /translation="MKTLLRLIRFGIILSLSLFIALSLYSWTKSIGQDKQRKELAVLL
                     KQTVEQEGVEAILSLSGVAVENIFHGEEGLILFEGSDTPWRYSADELQTISVYEKVNK
                     SVVNIT"
     gene            complement(368..>1104)
                     /locus_tag="PKN79_06840"
     CDS             complement(368..>1104)
                     /locus_tag="PKN79_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNZ95514.1"
                     /translation="KEPSLLAFASLDAAGKASYAFYWKNSAPVSMSVEELLKTMSDHT
                     DLRVVHIGSLALTLEPIGEVILEALERYEPKPIIFLDPNVRPSVIDDWNQYRTHIEKA
                     IDLASFVKLSDEDLQLIHPETEVHAKAAELARTKKKHIILTLGREGAVWYGPEGKEVS
                     HPIIDLPLADTVGAGDTFSGTILSFLHDRGYFGKEGEPPQLLEIPIKVRQEALKWASA
                     ASAINCSRPGCDPPSRKELLALLETL"
BASE COUNT          235 a          293 c          314 g          262 t
ORIGIN      
        1 ggtgatgttc acaacgcttt tattcacctt ttcgtaaacg gagatcgttt gcagctcgtc
       61 cgcgctgtag cgccagggag tatcgctccc ttcaaagagg atgaggcctt cttcaccgtg
      121 gaagatgttc tcaaccgcca ctccgctgag cgagagaatt gcttcaacac cttcttgttc
      181 caccgtttgc ttcaatagta cggcaagctc tttccgctgt ttgtcctgtc cgatcgactt
      241 cgtccatgag tacagggaaa gggcgatgaa caatgagagg ctcagaatga ttccgaagcg
      301 gatcaaacga agcagggtct tcatctttct ctggtcttta gacataaaaa cagtctactt
      361 ccaacaccta gagggtttca aggagcgcaa ggagctcttt ccgtgaaggg ggatcgcatc
      421 cggggcggct gcagttgatg gcggaggcgg cggaagccca tttgagcgcc tcttgacgaa
      481 ccttgatcgg aatttcgaga agttgcggcg gctcgccctc cttcccgaag tagccgcggt
      541 catgaaggaa ggagagaatc gttccgctga aggtatcgcc cgctcccacg gtatcggcaa
      601 gggggagatc gatgatcggg tgggaaacct ctttgccttc aggaccatac caaacggctc
      661 cttcccgacc caaggtgagg atgatgtgct tcttctttgt tcgggcaagc tcggcggcct
      721 tggcatgcac ttccgtttcc ggatgaatca gctgcaggtc ctcatcgctg agtttgacga
      781 acgaagcgag gtcgatcgct ttttcgatgt gggtgcggta ttgattccaa tcgtcaatca
      841 ccgatgggcg tacattggga tcgaggaaga tgatcggctt cggttcatag cgctccaggg
      901 cttcaaggat cacctccccg atcggctcga gggtcaatgc gagcgaaccg atatggacga
      961 ccctgagatc ggtatggtcg ctcatcgtct taaggagctc ctcgacgctc atcgataccg
     1021 gagcgctgtt tttccagtag aacgcgtagc tcgccttgcc cgccgcatcg agcgaggcaa
     1081 acgccaacaa ggagggctcc tttg
//
LOCUS       DAOPUZ010000456         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_158196_length_1095_cov_3.192308, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000456 DAOPUZ010000000
VERSION     DAOPUZ010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158196_length_1095_cov_3.192308"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /gene="der"
                     /locus_tag="PKN79_06845"
     CDS             complement(<1..1089)
                     /gene="der"
                     /locus_tag="PKN79_06845"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNZ95515.1"
                     /translation="MRPYGDRMVLTVNKVDDPKREDLVWEFYGYGYERVVGISAAHGL
                     GIDDLEELLLGMLDFEEIEQTEKEEATVKLAIMGKPNTGKSTLANLLVGRQISLVSEI
                     AGTTRDVVSGSFVHKGTTFNVLDTAGIRRKSRVDEDIEYYSVNRAIRTIEEADVVFLV
                     VDSAEGLADQDKKIAQLIVRRGKGVVIVLNKIDLLSGVPNELEAIRDRIRFLFPVLNF
                     APVMMISALEEKGIEPLLDTAWSLYRQLHRRIETSHLNEALRRWGEEYPPPRGREGHF
                     KVYYGTQVSTNPVNFLLFVNRMKEFPASYLTYLTNKIRSELGFPQIPVEIDLKERRRS
                     PSLNLKGPKPIQDREEKREVVPPGRTGGK"
BASE COUNT          216 a          325 c          299 g          255 t
ORIGIN      
        1 cttcccgccg gttcttccgg gaggaacgac ttcccgtttc tcttcacgat cttgaatcgg
       61 cttgggtcct ttaaggttca gcgagggcga acgtctccgc tccttcaggt cgatttcaac
      121 gggaatctga ggaaagccga gctcgcttcg aatcttgttg gtcagatagg taaggtagga
      181 agccggaaat tctttcatcc gattcacgaa gaggaggaag ttcaccggat tggttgacac
      241 ctgcgttccg tagtagacct tgaagtgtcc ttccctgccg cggggcggcg ggtactcctc
      301 gccccacctt cgcagcgctt cattcaggtg ggatgtttcg attcttcgat ggagctgacg
      361 gtacaggctc cacgcggtat caagaagcgg ctcgattccc ttctcctcga gggcgctgat
      421 catcatgacc ggggcgaagt tcaaaacggg aaagaggaaa cgaatccggt ccctgatcgc
      481 ttccagctcg ttgggaacac ccgagagcag gtcgatcttg ttgaggacga tgaccacccc
      541 ttttccgcgg cgaacgatca gctgagcgat cttcttgtcc tgatccgcca agccctcggc
      601 ggagtcgacg acgaggaaga cgacatccgc ctcctcgatc gtccggatcg cccggttcac
      661 cgaatagtac tcgatatcct catccactct gcttttacgc cggatcccgg cggtatccag
      721 aacattgaag gtcgtgccct tgtgaacgaa tgaaccgctt acgacgtcac gggtggtccc
      781 ggcgatttcg ctgaccagtg agatctgcct tccgacgaga agattcgcca gcgtggattt
      841 gccggtattg ggtttgccca tgatcgcaag cttgacggtt gcctcttcct tctcggtttg
      901 ctcgatctct tcaaagtcga gcatcccgag caggagctct tccaaatcat cgattccaag
      961 accgtgggcg gcgctgatgc cgacgacccg ctcatacccg tagccgtaga actcccaaac
     1021 caagtcctcc cgcttcgggt catctacctt gttgacagtg agaaccatcc gatcgccgta
     1081 ggggcgcagc gcctc
//
LOCUS       DAOPUZ010000457         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_159192_length_1091_cov_2.697876, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000457 DAOPUZ010000000
VERSION     DAOPUZ010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159192_length_1091_cov_2.697876"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..>1091)
                     /locus_tag="PKN79_06850"
     CDS             complement(3..>1091)
                     /locus_tag="PKN79_06850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNZ95516.1"
                     /translation="ETFKELAVEVAKKGKALSKRIFEFAQRCKVPEELESRLNELVVR
                     SNRIDSMVEVLLSFTDYGTWGEEVHWFNSERFGRDISVEVRITPLSVAPVLVEALFSK
                     LQTVVSTSATLDLHDEFEFWAKRVDLPYTDERPFFKGTFASPFEYTTHLLLITPEDAP
                     LPAKELEEEYLAYMSKTVLEATLASGGGALVLFTSYALLKELRSRLSEAYEEAGLLLL
                     SQGELDRHTLLRTFVGERDSVLFATSSFWEGVDAPGDTLRLVVITKLPFAVPSDPVFK
                     ARCDAIDAAGGSGFFELSLKNATMKLKQGFGRLIRSSTDRGVVLILDSRLVQRRYGQF
                     MLRSLPESFHQTGPSDSIAAKIERFLFP"
BASE COUNT          257 a          319 c          309 g          206 t
ORIGIN      
        1 gctcatggaa aaagaaaccg ttcaatcttg gcggcaatcg aatcggatgg acccgtctgg
       61 tggaaggatt cggggaggga acggagcatg aactggccat atcggcgctg aacaagtcgg
      121 gaatcgagaa tcagcaccac accccggtcg gtggaggagc ggatcagccg gccgaagccc
      181 tgtttgagct tcatcgtcgc attcttcagg gagagctcga aaaatccgct tccgccggcg
      241 gcgtcgatcg catcacaccg agctttaaag accgggtcgg agggaacggc gaagggaagc
      301 ttggtgatca cgaccagccg aagggtgtca ccgggggcat ccaccccctc ccagaaggag
      361 gaggtcgcaa acagaaccga atcccgctct ccaacaaagg ttctcaggag cgtatgccga
      421 tcaagctccc cttgactgag aagcaacagc cccgcttcct catacgcttc gctgaggcgc
      481 gatcggagct ctttgagtag cgcatagctg gtaaaaagca ccagagctcc accgcctgag
      541 gcaagcgtcg cctccagaac cgttttgctc atataggcaa ggtactcctc ctcaagctct
      601 tttgccggaa gcggcgcatc ctcgggggtg atcaagagca gatgggtcgt gtactcgaac
      661 ggtgaagcga acgttccttt gaagaacggg cgctcatcgg tatagggcag gtctacccgc
      721 ttcgcccaga actcgaactc atcatgcaga tccagcgtcg ccgacgtgga tacgaccgtc
      781 tgcagtttcg aaaagagcgc ttcgacaagg acgggggcga ccgagagcgg agtgatccgc
      841 acctcgaccg agatatcccg gccaaaccgc tcggagttga accaatggac ctcctcgccc
      901 cacgtgccgt agtcggtaaa ggaaagaaga acctccacca ttgaatcgat ccgattcgat
      961 cggacgacga gttcgttcaa tcgactctcc agctcttcgg gaactttgca gcgttgagcg
     1021 aattcaaaga tccgtttgct cagcgctttc cctttttttg ccacctcgac cgccagctcc
     1081 ttgaacgtct c
//
LOCUS       DAOPUZ010000458         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_159876_length_1088_cov_2.996128, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000458 DAOPUZ010000000
VERSION     DAOPUZ010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159876_length_1088_cov_2.996128"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PKN79_06855"
     CDS             complement(<1..1045)
                     /locus_tag="PKN79_06855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNZ95517.1"
                     /translation="MKLSYENKEREASILANTKAAVFDGKIGGGNLLIHGDNLNVLQA
                     LRASFSEKVDLIYIDPPYSTNTVYRVGSDRTGTVSFSPEDEVAYRDELGGSEYLESLR
                     KRLIIAKKLLSPRGSIYLHIDDKIGHYVNVLMDEIFGQENFRNDITRIKCNPKNFSRQ
                     GYGNIKDLILFYSNGPDPIWNEVRVPLDEADLNRLFRKVDKDGRRYTTIPLHAPGETR
                     DGATGKKFKGLLPPKGRHWRSSPEVLEKLDAQGLIEWSKNGVPRKIIYADEHIGKKLQ
                     DIWEFKDPQYPSYPTEKNLELLKTIVAASSNPESIVLDFYCGSGTALLASQQLGRRWI
                     GIDASLPAIETVGQ"
BASE COUNT          217 a          284 c          317 g          270 t
ORIGIN      
        1 gctgcccgac ggtttcaatc gccggaaggg atgcatcgat gccgatccat cgtcgaccga
       61 gctgctgaga agcgagtagg gcggtgcccg atccgcagta gaagtccaga acgatgctct
      121 ccggattcga ggaggctgca acgatcgttt taagcaattc gaggttcttc tcggtcggat
      181 aggagggata ctggggatct ttgaactccc agatatcctg aagctttttc ccgatatgtt
      241 catcggcgta gatgatcttt cggggaaccc cgttcttcga ccattcgatc aggccctgtg
      301 catcaagttt ttcgagcact tcgggcgagg agcgccagtg gcggcccttg gggggcagaa
      361 gtcctttgaa cttctttccc gtcgccccgt ctctggtctc gccgggggcg tggaggggaa
      421 tcgtcgtata ccggcggccg tccttgtcga ccttcctgaa aagccgatta agatccgctt
      481 catccaaagg aactctgact tcattccaga tcggatcagg tccgttgctg tagaagagga
      541 tcaggtcttt tatgttcccg tatccctgac ggctgaagtt cttggggttg cattttatcc
      601 gggtgatgtc gtttctgaag ttttcttggc cgaagatttc atccatcagg acgttcacgt
      661 agtggccgat cttgtcatcg atgtggaggt agatcgagcc ccggggggaa aggagctttt
      721 tggcgatgat caggcgcttg cgcagggatt cgaggtattc gcttccgcca agctcgtccc
      781 gataggcgac ctcatcctcc ggactgaagc tcaccgtccc ggttcggtcc gatccgactc
      841 ggtaaaccgt attggtggaa taggggggat cgatgtagat caagtcgacc ttctctgaaa
      901 aggaagcacg aagcgcctga aggacattga ggttgtcacc atggatcaga aggttccctc
      961 caccgatctt tccgtcaaac acggcggctt tggtgttcgc cagaatcgag gcctcacgtt
     1021 ccttgttctc atagctcagc ttcatcaccg catcctacca catgggcaag agggggctca
     1081 aggagagg
//
LOCUS       DAOPUZ010000459         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_161299_length_1082_cov_2.864654, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000459 DAOPUZ010000000
VERSION     DAOPUZ010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161299_length_1082_cov_2.864654"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1082
                     /locus_tag="PKN79_06860"
     CDS             <1..>1082
                     /locus_tag="PKN79_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNZ95518.1"
                     /translation="IEYASIKEYLQKRDALAFIGDERLLTSYKSLQSEAKSLYRTAFQ
                     IDRNVPKPNDLLLDYPLFVEENPAVIICDITRRAGGNHRFDFDGPRSYFGNFTLLKDD
                     LKALLRGGWKPTIFASNTLQRQRLEQMLSSIEGLAFEERELSGGFGIPSLQVLAICEH
                     EIFGRRREVVKTLRHTQTSPLDSFVDLNEGDYVVHVNYGIGRFEKIDRVSSFDRERDF
                     IKIVYADRENLYVPIEQANLIQRYIGSDGSPPRLDRLAGMGWETKKAKARKNAEDLAK
                     HLITLYAKRMNSVGYAFSKDTDWQLQFEASFAFDETADQLQCIEEIKEDMEKPIVMDR
                     LVCGDVGYGKTEIALRAAFKAVMDGK"
BASE COUNT          253 a          292 c          305 g          232 t
ORIGIN      
        1 aatcgagtat gcctcgatca aggagtacct ccaaaagcgc gatgcccttg cgttcatcgg
       61 tgatgaacgt ttgcttacgt cctataagag tcttcaaagc gaagcgaaaa gtctctaccg
      121 taccgcgttt cagatcgatc ggaacgtccc caaaccgaat gatcttctct tggattatcc
      181 tctcttcgtc gaagagaatc ccgcagtgat catctgcgat atcacccgcc gggccggcgg
      241 caaccatcgc tttgactttg acggtccccg atcatacttc ggaaatttca ccctcctcaa
      301 agatgatttg aaggcgcttc tgaggggagg gtggaagccg accatcttcg cctccaacac
      361 gctccagaga cagcgtttgg agcagatgct ttcctcgatc gaaggactgg cttttgaaga
      421 gcgggaactt tccggcggct tcggaattcc ctcgcttcag gtgttggcga tctgtgagca
      481 tgagatcttc gggcgaaggc gcgaggtggt caagaccctt cgacacaccc aaacatcccc
      541 cctcgattcc ttcgtcgatc tgaacgaagg ggattacgta gtgcacgtaa actacggaat
      601 cgggcgcttc gagaagatcg atcgggtctc ctcctttgat cgggagcggg acttcatcaa
      661 gatcgtctat gcggacaggg agaatctcta cgttccgatc gagcaggcga atctgatcca
      721 gcgttatatc ggcagcgacg gtagccctcc ccggttggac aggcttgccg gcatgggttg
      781 ggagacgaaa aaggccaaag cccggaagaa cgccgaagac ctggcaaagc atctcatcac
      841 cctctatgcg aagcggatga actcggtggg ctacgcgttc agcaaggaca ccgactggca
      901 gctccagttc gaggcctctt tcgccttcga tgaaacggcg gaccagctcc aatgcatcga
      961 ggagatcaag gaggacatgg aaaagccgat cgtcatggat cgtcttgttt gcggggacgt
     1021 cggttatgga aagacggaga tcgcattacg ggcggccttc aaagcggtga tggacggcaa
     1081 gc
//
LOCUS       DAOPUZ010000460         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_162217_length_1078_cov_4.909091, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000460 DAOPUZ010000000
VERSION     DAOPUZ010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162217_length_1078_cov_4.909091"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKN79_06865"
     CDS             <1..516
                     /locus_tag="PKN79_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNZ95519.1"
                     /translation="PGAYPGVGAEERGIGEAIARNLKVFSTLNVPILVFIIGEGGSGG
                     ALGIGVGDKLYMLESAVYSVITPEGFASILLRDPSRAREAAEAMKMTSRHLKEFKVIH
                     DIIEEGSAEETALRIKKIIIRDLDVLCAKPVNHLVRYRIRKIRGIGEVANLKAWWRPL
                     LEVFAKTETLR"
     gene            872..>1078
                     /locus_tag="PKN79_06870"
     CDS             872..>1078
                     /locus_tag="PKN79_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNZ95520.1"
                     /translation="MDNRPTIAIMYDFDKTLTTRAMQEYSFIPNLGMEAAAFWGKANE
                     LARKEGMDSVLAYMKLMLDEAKRHD"
BASE COUNT          227 a          289 c          343 g          219 t
ORIGIN      
        1 ccgggggcct atcccggagt cggggcggag gagcggggga tcggggaagc gatcgcccgg
       61 aacctgaagg tgttctcgac gctgaacgtc ccgatcctcg tcttcatcat cggcgagggc
      121 ggcagcggcg gagcgctggg gatcggggtc ggggacaagc tctatatgct ggaatcggcg
      181 gtgtactcgg tgattacccc cgaggggttc gcttcgatcc tgctgcgcga tccctcacgg
      241 gcccgggagg cggccgaagc gatgaagatg accagccgac acctcaagga gttcaaggtg
      301 atccacgaca tcatcgagga gggaagcgcc gaagagaccg cccttcggat caaaaagatc
      361 atcatccgtg atctggatgt gctctgcgcc aagccggtaa accacctcgt ccgctaccgg
      421 attcgaaaga tccgggggat cggggaggtc gccaacctca aggcgtggtg gcggcccctt
      481 ctggaggtct tcgccaagac ggaaaccctt cgttaaacct ccgctgcggg cgcccgtcgt
      541 tcgaggtttt tcagaaaata gataatagct cacaatactg tgaccggctc attgtgccgg
      601 acactgtctg gcatattgga tacttcaaat gtaatgtctc agggtttgca ggttggatct
      661 tgaaaaccca ccacgttgta cggcgcgttg acgaccattc cgacgatcgc cggaggctgc
      721 agcgggactc atggggccag ttcctttacc ggagcgattg taagagtttg gagcatatcc
      781 tgcatggccg tgttgtacaa aggaagcagt tgacgagtca atcaaccgtt tgctcttgcc
      841 ccgtgagctt ccccgggtat actcttttta catggacaac cggccgacga ttgcgatcat
      901 gtatgatttt gacaagacgc tgacgaccag ggcgatgcag gagtactcct tcattcccaa
      961 tctggggatg gaggccgccg ccttctgggg gaaggcgaac gagcttgccc ggaaggaggg
     1021 gatggactcg gtcctcgcct atatgaagct gatgctggat gaggcgaaac ggcacgac
//
LOCUS       DAOPUZ010000461         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_163045_length_1075_cov_2.443137, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000461 DAOPUZ010000000
VERSION     DAOPUZ010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163045_length_1075_cov_2.443137"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PKN79_06875"
     CDS             complement(<1..600)
                     /locus_tag="PKN79_06875"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNZ95521.1"
                     /translation="MSLNWREIELIVSELPLKGSRLQGVTQHDFNAISFHFFHPVHRQ
                     WTLYTEVGTPSSRLHKLTRARAKLPKTQRFVQFLKANLVNCLVSEVAQTPGERFVDLT
                     CTRGEDTMHLLLRFYSGAGANIIVTDDSYTILDLLLRRPRRGEASGLSFALPEPKEID
                     DAFPIRRRTKESFNEQIEEEYAAERNIRTLESLYERATLL"
     gene            complement(597..>1075)
                     /locus_tag="PKN79_06880"
     CDS             complement(597..>1075)
                     /locus_tag="PKN79_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNZ95522.1"
                     /translation="TLGLFGGRTIHALEANTVPGRFQQVAPSLYIDGAHTPASLSALT
                     RSFDELYPSKDANVVIYGALEDKDHPHMASIVLQHFNKIIISRPGTFKKSDLQKLFSI
                     FEALKAESRSTASIFLIEDNSQALEKAYMLKGEDGAILVCGSFYLGGGIAEALKRR"
BASE COUNT          228 a          283 c          318 g          246 t
ORIGIN      
        1 gagaagcgtt gcccgttcat acagcgactc aagtgtccgg atgttccgct cggcggcata
       61 ctcctcttcg atctgctcgt tgaaggattc cttcgtcctc ctgcggatag gaaatgcatc
      121 atcgatttct ttcggttcgg gcaaggcgaa ggacagaccg cttgcttcgc cccggcgggg
      181 acgccggagg agaagatcca gaatcgtgta cgagtcgtcg gtcacaatga tgttcgcccc
      241 cgctccgctg tagaaccgca aaagcagatg catcgtatct tcgccccggg tgcacgtcag
      301 atcgacgaac cgttcacccg gggtctgggc aacctccgag accagacagt tgacaaggtt
      361 cgctttgagg aattggacga agcgctgcgt tttgggcagt tttgcccgag ctcgggtaag
      421 cttgtggagc cgcgaggaag gggttcccac ctcggtatag agcgtccatt gccggtggac
      481 aggatgaaag aaatggaagc tgatcgcgtt gaagtcgtgc tgggtcaccc cttgaagcct
      541 tgagcccttc aaggggagct cgcttacaat gagttcgatt tcacgccaat tcaagctcat
      601 cttctcttga gggcctccgc gattccacct ccaaggtaga atgatccgca caccagaatc
      661 gctccgtctt ctcccttcag catatacgct ttctcgagcg cttggctatt gtcctcgatc
      721 aggaagatcg aggcggtgga gcgggattcc gctttcaagg cttcaaagat ggaaaaaagc
      781 ttttgcagat cgcttttctt gaaggttccc ggccgggaga tgatgatctt gttgaagtgt
      841 tgaaggacga ttgacgccat gtgaggatga tccttgtctt caagagcgcc atagatgacc
      901 acgttcgcat cttttgacgg atacagctca tcaaaggatc gggtcaatgc gctcagagaa
      961 gcgggggtat gcgctccgtc gatatagagg gagggggcga cctgctgaaa gcggccgggc
     1021 accgtgttcg cttcgagggc atggatcgtc ctcccgccaa agaggccaag ggtcc
//
LOCUS       DAOPUZ010000462         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_163724_length_1072_cov_3.135693, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000462 DAOPUZ010000000
VERSION     DAOPUZ010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163724_length_1072_cov_3.135693"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PKN79_06885"
     CDS             complement(<1..223)
                     /locus_tag="PKN79_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95523.1"
                     /translation="MARSFLSTLFGTKQEKDLKHLYPIVQQVNDLEPWAQGLADDQFP
                     DETQKLKDALRQGATLDDLVPKAFALAREA"
     gene            365..>1072
                     /locus_tag="PKN79_06890"
     CDS             365..>1072
                     /locus_tag="PKN79_06890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ95524.1"
                     /translation="MVVCFTGGGTLGHVYPALAVHQELSSSDSYSGFWIGRNDPTERR
                     IVEEANLDFYSIPSGKLRRYPSLRTVTDGFKVLGAIGFAYRILKRRKPDLLFSKGGFV
                     SVPPVIAAHLLKIPVISHESDISPGLATRINARFSDLICVPFAEGFDRLGREKLRVTG
                     NPVRRDLLEARQMPFSRPEGDPPLLLVLGGSGGSGAINALIGKTLDELLTICSVYHQV
                     GANAPLPSLRPNYTAVEF"
BASE COUNT          229 a          280 c          309 g          254 t
ORIGIN      
        1 cggcttcccg ggcgagagcg aatgccttgg gaacaagatc gtccaatgtc gctccctgcc
       61 tgagagcatc cttcagcttc tgcgtttcat cggggaattg gtcgtcggca agaccttgcg
      121 cccaaggttc caggtcattc acctgctgaa cgatcggata gaggtgcttc aagtctttct
      181 cttgcttggt tccaaagagg gtggataaaa atgaacgagc catatgatac ctgccttggt
      241 atgagtatac tcatttggga taattccgaa tagagaaaac gatgagatgg ggcaaatata
      301 cccatagggt caaattgata caaatccgct gtacgagctc attggaagat gatatgctcg
      361 aggtatggtt gtgtgcttta ccggcggagg gacccttggg catgtctatc ccgcgctggc
      421 cgttcaccag gagttgtcaa gctccgattc ctactcgggc ttttggatcg gtcgaaacga
      481 tccgacggaa cgaaggatcg tggaagaagc gaacctcgac ttctattcga ttccttcggg
      541 caagctgcga agatatccct cccttcggac cgtcaccgac ggattcaagg tgctgggggc
      601 gatcggattc gcataccgga ttctgaagag aaggaaaccc gatctcctgt tcagcaaagg
      661 cgggtttgtc tccgtccccc cggtgatcgc ggcgcatctt ttgaagattc cggtgatcag
      721 ccatgaaagt gacatcagcc cggggctggc gacgcggatc aacgcaagat tctcggatct
      781 gatctgtgtt cctttcgccg aaggcttcga ccggctggga cgggaaaaac tgagggtgac
      841 gggcaatccc gtccgcaggg atcttcttga agcgaggcag atgcctttca gtcgtcctga
      901 gggcgaccct ccgcttctgt tggttctcgg cggatcgggc ggtagcggtg cgatcaacgc
      961 cctcattgga aagacccttg atgagctgct tacgatctgc tcggtatacc atcaggtggg
     1021 ggcaaatgcc cctctgcctt cgctccggcc caattatacc gccgtcgagt tc
//
LOCUS       DAOPUZ010000463         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_164023_length_1071_cov_2.680118, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000463 DAOPUZ010000000
VERSION     DAOPUZ010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164023_length_1071_cov_2.680118"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PKN79_06895"
     CDS             <1..433
                     /locus_tag="PKN79_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ95525.1"
                     /translation="SVMAKVEVKKIPIINLWCKAMKIIFIDRKSPHDSIKAIFTGVEH
                     LKSGGNLLIFPEGTRSKTGKIGELKAGSIKLATRSRATIVPFAIKNLRPGFEGITNLK
                     RVHASMVIGKPIETKNLSKEELAGLHEVVYGAITDLFDQLP"
     gene            396..>1071
                     /locus_tag="PKN79_06900"
     CDS             396..>1071
                     /locus_tag="PKN79_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ95526.1"
                     /translation="MERSPISSINSLDVTNLSFSYQGWAGENPPSLFENLSFSIPRGS
                     KALLLAPFDSGKSTLGRMLVGGVPKYFPGRLEGAIKVMGHNLADIEVWDLLDLVTLVS
                     QNPQEQFVASTVEEEVAFGLESLGLPPKEMRRRVEDVLTNWGLDVLRQVSNSELSGGE
                     RKRVLLAAAEALDAPFWILDEAFDDLDLRWREHLARFIQTTDKTILLFSSRYLAQFDR
                     LFDHVFL"
BASE COUNT          248 a          273 c          294 g          256 t
ORIGIN      
        1 gtcggtgatg gcgaaagtgg aagtcaagaa gattccgatt atcaacctct ggtgcaaggc
       61 gatgaagatc atcttcatcg atcgcaaaag ccctcacgat tcgatcaagg cgatttttac
      121 gggagtcgaa catttgaaga gcggtggaaa ccttctgatc ttccccgagg gaacccgttc
      181 aaaaaccgga aaaatcgggg aactgaaagc cggctcgatc aagctggcga cccgcagccg
      241 ggctacgatc gttccgttcg cgatcaagaa ccttcggccc ggatttgagg ggatcaccaa
      301 tctcaagcga gtccacgctt ccatggtcat cgggaagccg atcgagacga aaaatctgag
      361 caaagaggag cttgcaggtc tgcatgaggt agtctatgga gcgatcaccg atctcttcga
      421 tcaactccct tgatgtaacg aatctctctt tttcctatca aggatgggcg ggcgagaatc
      481 caccatcact ctttgagaat ctgtcctttt ccattccaag aggaagcaaa gccctcttgc
      541 ttgctccctt tgacagcggc aaatccaccc ttgggcggat gcttgtcgga ggagtcccga
      601 aatacttccc cgggaggttg gaaggcgcca tcaaggtcat ggggcataac cttgccgata
      661 tcgaagtctg ggatcttctc gatctggtga cgctcgtttc tcaaaacccg caggagcagt
      721 tcgtcgcttc gaccgtagaa gaagaggtcg cctttggatt ggaatcattg ggtcttccgc
      781 cgaaagagat gagaagacgg gtggaggacg ttctgacaaa ctggggtctc gatgtattgc
      841 gacaggtttc caacagcgag ctgtccggag gggagcgcaa gcgggtgctc ctcgccgccg
      901 ccgaggcgct tgatgctcct ttttggattc tggatgaagc gtttgatgat ctcgacctgc
      961 gatggcggga acacctggca cggttcattc aaaccaccga taagacgatt cttctcttct
     1021 cttcccggta tcttgcccag ttcgatcgat tgtttgatca tgtttttctg c
//
LOCUS       DAOPUZ010000464         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_165263_length_1066_cov_3.444115, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000464 DAOPUZ010000000
VERSION     DAOPUZ010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165263_length_1066_cov_3.444115"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKN79_06905"
     CDS             complement(<1..539)
                     /locus_tag="PKN79_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ95527.1"
                     /translation="MKMLQIPKIGMGTFGSDHISANVMASSVKEALAIGYRMIDCARV
                     YDNEREIGEVLAASKIKRSDLWLTGKVWNDMHGKANVIKSAELSMKDLQTDYLDLFLV
                     HWPFPNHHPPFAHVDERNPNAVPYIHKQFMDTWSGMEELQRKGMVRHIGVSNMTEAKL
                     RLLLGDCSIRPFAAELELHP"
     gene            628..>1066
                     /locus_tag="PKN79_06910"
     CDS             628..>1066
                     /locus_tag="PKN79_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNZ95528.1"
                     /translation="MKHIGLDLRFQDGYDMEVAGGVVRYARSKSDWILRGQGPWFSFK
                     EDLSECDGLIARIESKEEAKEVRGYRIPVVDIASAVRLPTLAQVHNDDHLTGKRAGSY
                     LKGLGIEHFGYCSAEGLIWAKERLRGYQEEVQREIFHFSRPLGW"
BASE COUNT          218 a          289 c          313 g          246 t
ORIGIN      
        1 ggatggagct ccagctccgc cgcgaagggg cggatcgagc aatccccaag gagcaggcgc
       61 agcttcgcct cagtcatgtt gctgacgccg atatgtctta ccattccttt tcgctggagc
      121 tcttccatgc cgctccacgt atccatgaac tgcttgtgaa tatagggaac cgcgttgggg
      181 ttgcgctcgt ccacatgcgc aaagggcggg tgatggttgg gaaacggcca gtggacgagg
      241 aagagatcaa ggtaatcggt ctgaagatcc ttcatgctca gttcggcgga cttgatgacg
      301 ttcgccttcc cgtgcatgtc attccagacc ttgccggtga gccacaggtc gctgcgcttg
      361 atcttgcttg ccgcgagcac ttctccgatc tcccgctcat tgtcgtacac ccgggcgcag
      421 tcgatcatcc gatacccgat cgccagcgct tccttcaccg aagaggccat cacgttggcg
      481 gaaatatggt cggaaccgaa cgtacccata ccgatcttgg gaatttgtaa catcttcatg
      541 tcttaacggt atcaaagcgg gcaggaaata caatgcttga tatacgaata tcttgtatcg
      601 ttttgcgaat tcatctattc tggatccatg aagcacatcg gtttggatct tcgttttcag
      661 gatggctacg acatggaggt cgccggcgga gtcgtccgat acgctcgaag caagagcgat
      721 tggatccttc ggggtcaagg cccctggttc tccttcaagg aggatctttc cgagtgtgac
      781 ggtctcattg cccggatcga gagcaaggag gaagccaagg aggtccgcgg ctatcggatt
      841 ccggtcgtcg acatcgcttc ggcggtccgc ctgccgaccc ttgctcaggt gcacaacgac
      901 gatcatctta ccggaaaacg agcggggtcc tatctgaagg ggctggggat cgagcatttc
      961 ggctattgca gcgccgaagg cctgatctgg gcaaaggagc gcctcagggg gtatcaggag
     1021 gaggttcaac gggaaatctt ccacttctcc cgcccgctgg ggtggt
//
LOCUS       DAOPUZ010000465         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_166334_length_1062_cov_2.210526, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000465 DAOPUZ010000000
VERSION     DAOPUZ010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166334_length_1062_cov_2.210526"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKN79_06915"
     CDS             complement(<1..687)
                     /locus_tag="PKN79_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNZ95529.1"
                     /translation="MNNTFDRLHNDLLMISNPARYIGGEYIQNRKEPKEGDLRCAVCF
                     PDLYEIGMSNLAIKILYDQLNRIEDVHCDRVFSVAPDFEALLREKNLPLYTLQTRLGL
                     HELDFLGISIGYELTATNILQVLDLGRIPIRAGERGDEHPIVIGGRPAITNPLPFGAF
                     LDLVYIGESEGGLEAVVAVLRQAKREGWSRAEKIRELRQFTFLWHLGKKSTVRHIDHA
                     FPEKRTFQHFV"
     gene            complement(680..>1062)
                     /locus_tag="PKN79_06920"
     CDS             complement(680..>1062)
                     /locus_tag="PKN79_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95530.1"
                     /translation="AKEYYRALLKTGRTQEAKTLFSEAIRGTLSPFEEAKLLIASEVD
                     PAALETHLGMLSIEEQVNLVLEVVPLGLDPSIAEAWFISLKMQKSDTIELYRALALLA
                     GRAGRRYEEAQYARLYQKNKEAHE"
BASE COUNT          231 a          291 c          279 g          261 t
ORIGIN      
        1 gacaaagtgc tgaaacgtcc gtttttcagg aaaggcgtga tcgatatgcc gcacggtgga
       61 ttttttaccc aaatgccata agaacgtgaa ttgtcgcaac tccctgattt tttccgcccg
      121 actccaccct tccctctttg cttgccgtaa tacggcgacc acggcctcca gaccgccttc
      181 cgattcgccg atatacacga ggtcaaggaa tgctccgaac ggcagcggat tggtgatcgc
      241 cggcctgccc ccgatcacga tcggatgctc gtccccccgc tcgcctgccc tgatcggtat
      301 cctgcccaga tccaagactt gaaggatgtt cgtcgccgtc aattcatagc cgatcgagat
      361 gccgagaaaa tcgagttcat gaagaccaag ccgggtctgg agcgtgtata acggaagatt
      421 tttctccctg agcagcgctt caaagtcggg agcgacggaa aagacgcggt cgcaatggac
      481 gtcctcgatg cgattcagtt gatcgtagag gatcttgatc gccagattgc tcatcccgat
      541 ctcatagagg tcgggaaaac agaccgcaca tcgaagatcg ccttctttgg gctccttcct
      601 gttttggata tattcaccgc cgatataccg ggcggggttg cttatcatca acaagtcatt
      661 gtgtaatcga tcgaacgtat tattcatgag cttccttgtt tttttggtaa agccgggcgt
      721 actgtgcttc ctcgtatcgc cgcccggctc gaccggcaag cagagccaag gcgcgataaa
      781 gctcgattgt atcactcttt tgcattttta gggagatgaa ccacgcttcg gcgatcgagg
      841 gatccaatcc aaggggaacc acctccaata cgaggttgac ctgctcttca attgaaagca
      901 tgcccagatg ggtttcaagg gcggcgggat cgacttcgga ggcgatgagg agcttcgcct
      961 cctcgaacgg tgaaagggtt ccccggatcg cttctgaaaa gagggtcttt gcttcctgcg
     1021 tcctgcctgt tttcaggagc gcccggtaat actcttttgc gt
//
LOCUS       DAOPUZ010000466         1058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_167215_length_1058_cov_3.515454, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000466 DAOPUZ010000000
VERSION     DAOPUZ010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167215_length_1058_cov_3.515454"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PKN79_06925"
     CDS             complement(<1..725)
                     /locus_tag="PKN79_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(A) polymerase"
                     /protein_id="HNZ95531.1"
                     /translation="MLIRYKQDDQGNTLPIANIYTQAEHKIETRNIDRDALWVIKKLQ
                     GSGAEAYLVGGAVRDLMLGNIPKDFDIATSASPRQVQRLFYNARIIGRRFKLVHLIFK
                     DKVLEVSTFRSGEEAEDGSNNVFGSIEQDAKRRDFSINSFYYDPSNGQLLDFNDAMED
                     MRKKRISSVIPLEESFTEDPVRMIRAVKYAVITGFSLRFKLRVALRKYAPELSRISSS
                     RLTEELVKILSSGHAAEIFADLNR"
     gene            827..>1058
                     /locus_tag="PKN79_06930"
     CDS             827..>1058
                     /locus_tag="PKN79_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNZ95532.1"
                     /translation="MKDQEIIQPRVLKGFRDSLPSAQIPKEALVSTLQKHFSSYGFVP
                     IDTPALEYMEILLGKGGGETDKQIFHFTDQGGR"
BASE COUNT          241 a          276 c          290 g          251 t
ORIGIN      
        1 cggttaaggt ctgcgaaaat ctccgccgca tggccgcttg aaaggatttt tacaagctcc
       61 tccgtcaggc gggaggagga gatccgtgaa agttccggag cgtatttcct gagtgcgacg
      121 cggagcttga agcgcaatga aaagccggta atgaccgcgt atttcaccgc ccggatcatc
      181 cgaaccggat cttcggtgaa ggactcctcc agagggatca cgctgctgat ccgctttttc
      241 ctcatatcct ccatcgcgtc gttgaaatcc agcaactggc cgttggatgg atcgtagtaa
      301 aagctgttga tggagaaatc gcggcgcttg gcgtcctgtt cgatcgaacc gaagacgttg
      361 ttcgatccgt cttccgcctc ttcgccgctg cggaacgtcg atacttccag gactttgtcc
      421 ttgaagatca agtgaacaag cttgaagcgc cggccgatga tccgggcatt gtaaaaaagc
      481 cgttggacct gccgaggcga ggcgctggtg gcgatgtcga aatctttagg gatgttcccc
      541 agcatcagat cacggaccgc cccgccgacg aggtaggctt cggccccgct gccctgaagc
      601 ttcttgatca cccacagagc atcgcggtcg atgttccggg tttcgatctt gtgttccgcc
      661 tgggtataga tgttggcgat cggaagggtg ttgccctggt catcttgttt atatcttatc
      721 agcatcggta tctcgaaaat tgggaggata ttgcagtgaa cgaatgattc gtattacact
      781 ctttctacac catccaacga caaaacacaa caggagcagc agggtaatga aggatcagga
      841 gatcatccag ccaagagtct tgaaaggatt tcgggacagt cttccttctg cccagattcc
      901 gaaagaagcg cttgtgtcca ccttacagaa gcatttttcc tcctatgggt tcgtcccgat
      961 cgataccccg gctcttgaat acatggaaat tctcctcgga aaaggcggcg gagaaacgga
     1021 caagcagatc ttccacttca ccgatcaggg gggtcggg
//
LOCUS       DAOPUZ010000467         1057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_167500_length_1057_cov_2.950100, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000467 DAOPUZ010000000
VERSION     DAOPUZ010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167500_length_1057_cov_2.950100"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..>1057)
                     /gene="prfB"
                     /locus_tag="PKN79_06935"
     CDS             complement(24..>1057)
                     /gene="prfB"
                     /locus_tag="PKN79_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNZ95533.1"
                     /translation="ESSSIRKEIQTLEERTLQPTFWDDRKRAEETFSRLNSLKDSYEP
                     WKELIEEIDDLTELVELYADEPDNAEEQEQVDAHIAAVADTYQKLKLKTLLDGEFDKN
                     DTFLTIHAGAGGNEASDWAQMLMRMYLRYSELMDFKTQILDLQEDEGGIKSASIKISG
                     PYSFGYLKGEAGVHRLVRISPFDSQKRRHTSFTSVYASPVIDDEIEIDLKPEDYRLDT
                     YRAGGAGGQHVNKTDSAVRITHFESGIVVQCQNERSQIMNKEVAFKMLRSRLYEYYRA
                     IRDEERAKNAIEKKDIAWGSQIRSYVFQPYTMVKDHRTNESVGNIQAVMDGQIHPFIE
                     AYLQWQHSE"
BASE COUNT          199 a          304 c          265 g          289 t
ORIGIN      
        1 atcgaaccct tgcctttcat cgcttactcc gaatgttgcc actgcaggta tgcttcgatg
       61 aatggatgga tctgtccatc catcactgcc tgaatattac cgacgctctc attggttcgg
      121 tggtctttca ccatcgtata cggctgaaat acgtaggagc ggatctggct tccccaggcg
      181 atatccttct tctcgatcgc atttttcgcc cgctcttcat cccggatcgc ccggtagtat
      241 tcatacaagc ggctgcgaag catcttgaag gcaacctctt tgttcatgat ctgggaacgc
      301 tcattctgac attgaacgac gatccccgat tcaaagtggg taattcgaac cgcgctgtcg
      361 gtcttattga cgtgctggcc tcccgctccc ccggcccggt aggtatcgag gcgataatcc
      421 tccggcttca agtcgatctc gatctcatca tcgatgaccg gagaggcgta cacgctggta
      481 aaggaggtgt gtcttcgttt ttgggaatcg aaggggctga tccgcaccag acggtgaact
      541 cccgcctccc ctttcaaata tccgaagctg tacggtccgg agattttgat ggaagcgctt
      601 ttgattcccc cctcatcctc ctggagatcg aggatctggg tcttgaaatc catcaattcg
      661 ctgtagcgca agtacatccg catcagcatc tgggcccagt cactcgcttc attccccccg
      721 gccccggcgt ggatcgtcaa gaacgtatcg ttcttgtcga actcgccgtc cagaagggtt
      781 ttgagcttca gtttttgata tgtatcggct accgcggcga tgtgggcatc aacctgttcc
      841 tgctcttcgg cgttgtccgg ctcatccgca tagagctcaa ccagctcggt gaggtcgtcg
      901 atctcttcga tcagctcctt ccaaggctca taggagtctt tcagggaatt gagccggctg
      961 aacgtctctt cggcccgctt ccggtcatcc cagaatgtcg gttggagtgt ccgttcctcc
     1021 agggtttgaa tctctttccg gattgaggag ctttcaa
//
LOCUS       DAOPUZ010000468         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_168154_length_1055_cov_1.812000, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000468 DAOPUZ010000000
VERSION     DAOPUZ010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168154_length_1055_cov_1.812000"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PKN79_06940"
     CDS             <1..656
                     /locus_tag="PKN79_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ95534.1"
                     /translation="VLQKGFHHEDVLQHSFTTCQKAADIGSSVHVRLAALFHDIGKTT
                     TREPNGDRYSFYNHEIVGEALTKTILRRLKASNEEIDTVSHLVRHHMFSYTPEMTDSA
                     VRRFIVRVGIEHIEELFKLRIADQMAISGRADTDLLDEFSKRINGIISSDDALSIKDL
                     AIGGNDLLALGIPKGKRIGQTLQYLLETVLDDPAQNTKEQLFRIALAYQELCGFTSD"
     gene            complement(626..>1055)
                     /locus_tag="PKN79_06945"
     CDS             complement(626..>1055)
                     /locus_tag="PKN79_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HNZ95535.1"
                     /translation="KLNVAQYLLLGKGEEQTGGRRKKALLADCTEALFGACYVHNGFP
                     AAKAFVSRALGERITAVVEDDYHRDYKTALQEYMQKKWRIVPTYRLVSKTGPDHNFTF
                     YVEVDVNGQVFGPAHGRSKKAAEQAVAKIAYQSLVNPQSS"
BASE COUNT          239 a          320 c          263 g          233 t
ORIGIN      
        1 gagtcctgca aaaagggttc caccatgagg atgtcctcca gcactccttc accacctgcc
       61 aaaaagcggc ggacatcggc tcctccgtgc atgttcgcct tgcagccctc tttcatgaca
      121 tcggcaaaac aaccacccgg gaaccgaacg gcgaccgcta ctctttttac aaccatgaga
      181 tcgtgggaga agcgctcacg aaaacgatcc tcaggcgcct gaaggcctcc aacgaggaga
      241 ttgataccgt ctcccatctg gtgcgccacc atatgttctc ctacaccccc gagatgacgg
      301 acagcgcggt acgccgcttc atcgttcgag tcggaatcga gcatattgaa gagctcttca
      361 agctgaggat cgccgatcaa atggcgatca gcggccgggc cgataccgat cttctggatg
      421 aattttccaa gcgaatcaac ggaattatct catccgacga tgccctctcc atcaaagatc
      481 ttgcaatcgg gggaaacgac ctcttggcgt tgggaatacc caagggcaag cggatcggtc
      541 aaacgctcca atacctgctg gagaccgtac tggatgatcc cgctcaaaac acgaaagagc
      601 agctcttcag gatcgctctg gcctatcagg agctctgtgg atttaccagc gattgatatg
      661 cgattttagc caccgcctgc tcggcggcct tcttgcttcg cccatgggcg ggaccgaaga
      721 cttgtccgtt cacgtccact tcgacataga aggtgaaatt atgatcgggt ccggtctttg
      781 acacgagcct gtaggtgggt acgattcgcc acttcttctg catgtactct tgaagagccg
      841 ttttatagtc gcgatgataa tcatcttcga cgacagcggt aatccgttct ccgagggctc
      901 gactgacgaa cgccttcgcc gccggaaagc cgttgtgcac atagcacgcc ccaaagagcg
      961 cttccgtgca atccgccaag agcgctttct ttctccgccc gccggtctgc tcctccccct
     1021 ttcccagaag gagatattga gcgacgttca gcttc
//
LOCUS       DAOPUZ010000469         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_168549_length_1053_cov_2.887776, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000469 DAOPUZ010000000
VERSION     DAOPUZ010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168549_length_1053_cov_2.887776"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1053
                     /locus_tag="PKN79_06950"
     CDS             <1..>1053
                     /locus_tag="PKN79_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNZ95536.1"
                     /translation="GKFKSIRYKGVICDRCNVEVTNTKVRRERMGHITLAAPVSHIWY
                     YRSVPSRMSMLLDITRNALQSILYYEKYVVINSGDTTLKPKQLLTEEEYWDAREQFGD
                     SFEAGMGAEAVKKLLTNLNLEELSRELREQMRQKADKADKRLLKRIEVCENFRDSGNR
                     PEWMILTVIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLDRLVKLNAPDII
                     IRNEKRMLQEAVDALFDNSKRKRVVKGASNRPLKSLSDMLKGKQGRFRQNLLGKRVDY
                     SGRSVIVVGPELRMHQCGLPSKMALELYKPFIMKKLVQDGVVYNIKKAKSLVEEETDA
                     VWAILDEVVKEHPVLL"
BASE COUNT          238 a          304 c          322 g          189 t
ORIGIN      
        1 gcggaaagtt caagtcgatc cgctacaagg gcgtgatctg tgaccgctgc aacgtcgagg
       61 tcaccaatac gaaagtccgg cgcgagcgga tggggcacat cactctcgcc gctccggtca
      121 gccatatctg gtactatcgc tccgtcccct cgcggatgag catgctgctg gatatcaccc
      181 ggaacgcgct gcagtcgatt ctctactatg aaaaatatgt cgtcatcaac agcggcgaca
      241 cgaccctcaa gcccaagcag ctcctcaccg aagaggagta ttgggacgcc cgcgagcagt
      301 tcggcgactc cttcgaagcg ggaatgggcg cggaagcggt caagaagctg ctgacgaacc
      361 tcaatctgga ggagctcagc cgggagctgc gcgagcagat gcgccaaaaa gcggacaagg
      421 cggacaagcg cctcttgaag cggatcgagg tgtgcgagaa cttccgcgac agcggaaacc
      481 gcccggagtg gatgatcctt accgtgatcc ccgtgattcc gccggatctg aggccgatgg
      541 tccagctcga cggcgggcgt ttcgccacca gtgatttgaa cgatctctat cgaagggtga
      601 tcaaccggaa caaccgcctc gatcgcctcg tgaagctcaa cgccccggat atcatcatcc
      661 ggaacgagaa gcggatgctt caggaagcgg ttgatgcgct ctttgacaac tccaagcgga
      721 agcgggtcgt gaagggtgcc tccaaccgcc cgctgaagag cctttcggac atgctcaaag
      781 gaaagcaggg gcgcttccgg cagaacctcc tcggaaagcg ggtcgactac tccggccgtt
      841 cggtcatcgt cgtcggtccg gagcttcgga tgcaccagtg cggtcttcct tcgaagatgg
      901 cccttgagct ctacaagccg ttcatcatga agaagctggt ccaggacggg gtcgtctaca
      961 acatcaagaa ggcgaagagc ctcgtcgaag aggagacgga tgcggtctgg gcgatcctgg
     1021 atgaggtcgt caaggagcac ccggttcttc tta
//
LOCUS       DAOPUZ010000470         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_168824_length_1052_cov_2.684052, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000470 DAOPUZ010000000
VERSION     DAOPUZ010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168824_length_1052_cov_2.684052"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PKN79_06955"
     CDS             complement(<1..1033)
                     /locus_tag="PKN79_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ95537.1"
                     /translation="MAHHGPEDFLISTATIVGSYILMFTYSPPLALISLIPFPFMVFY
                     GSHYGRKMRATFRRVRSTVADVNSNVENSLMGIREVKSFGRETFQTEKFQEVNNILKD
                     SKMQQYKVMGSYHALMGLFRNLYYFFTVVGGAILILRGTIEYYDLITFILYVGIVLPP
                     IDRLINFTEQLQQGMASFERFVQIMDEHPAIQDAKDARELTVKDGTIVYENVSFTYEN
                     TDTAVLSDVNMTISGGETVAIVGESGAGKSTFASLLPRFYEPQAGRILIDGTDITEVR
                     QSSLRKEIGFVQQNVFLFDDTIRENLRYGKVDATDEQLWAALDAANLGEFVRSLPLGL
                     ETQVGERGTL"
BASE COUNT          244 a          295 c          282 g          231 t
ORIGIN      
        1 gcagcgtccc ccgctcccct acctgcgttt caagacccaa ggggagggag cggacgaact
       61 cgccgaggtt cgccgcatcg agcgccgccc aaagctgttc gtcagtggca tcgaccttcc
      121 cgtagcggag gttctccctg atcgtgtcat cgaagagaaa gacgttctgc tgaacaaagc
      181 cgatttcctt gcggagcgat gattgccgaa cctcggtgat atcggtgccg tcgatcagaa
      241 tccgacctgc ttgcggttca tagaaacggg gaagaagtga agcgaacgtg gattttcccg
      301 ctcccgattc tccgacgatc gcaacggtct ctccacctga aatcgtcatg ttgacatcgc
      361 ttaagaccgc cgtatcggta ttctcatagg tgaacgagac gttctcatag acgatcgttc
      421 catccttcac cgtcagctct ctggcatcct tggcatcctg gatagccgga tgctcgtcca
      481 tgatctggac aaagcgctca aagctcgcca tcccctgctg aagctgttcg gtgaagttga
      541 tcaagcggtc gatcggagga agaacgatgc ccacgtagag aatgaaggtg attaggtcgt
      601 agtactcgat cgtccctctc aggatgagaa tcgcccctcc gactacggta aagaagtaat
      661 aaagattcct gaagagcccc atcagggcgt ggtaggaacc catcaccttg tactgctgca
      721 tcttcgagtc tttgaggatg ttgttcacct cttgaaactt ctccgtctga aaggtctccc
      781 ggccgaacga tttgacttca cggattccca tcagtgagtt ttccacgttg ctgttcacat
      841 cggcgacggt cgagcggacc cgccgaaacg tagcccgcat cttacgtccg taatgcgacc
      901 cgtagaacac catgaacgga aacgggatca gactgatcag ggcgagcggc ggactgtagg
      961 tgaacatcag gatataggaa ccgacgatcg tcgctgtgct gatcagaaaa tcctcagggc
     1021 catggtgcgc cacctcggcg atgatgaaca aa
//
LOCUS       DAOPUZ010000471         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_171898_length_1040_cov_3.214213, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000471 DAOPUZ010000000
VERSION     DAOPUZ010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171898_length_1040_cov_3.214213"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..810
                     /locus_tag="PKN79_06960"
     CDS             145..810
                     /locus_tag="PKN79_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNZ95538.1"
                     /translation="MKTESWLQKHITLHDYDDPKAERANALTHGFGAILAVLALGMVI
                     GRFSFYSSGSLARGMVLFCLTMILLYSSSTLYHLAPKSDAKRILRVFDHSSIYVLIAG
                     TYTPLMLGIDSDTARMVLFLVWTIAVAGIILTFLFWGKFKVLRVLLYVVMGWLVVFFW
                     GDIIPFLPQGLFPYVLAGGITYTVGVAFYASKRLPCHHAVWHLFCIGGSVPFFIGFFV
                     KLR"
     gene            complement(782..>1040)
                     /locus_tag="PKN79_06965"
     CDS             complement(782..>1040)
                     /locus_tag="PKN79_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HNZ95539.1"
                     /translation="LDSLDGERESLATLKTFIRKQKMDESSVFYDEGIFAYIFQTSSL
                     PTTVFFYGDGAIAGGQLGMVNEQYLQYAIEEITSEASRKSR"
BASE COUNT          211 a          297 c          262 g          270 t
ORIGIN      
        1 cctccggatg agctgttctc caagcccaaa agcgatcgga cgaagcattt tctggaacat
       61 atcctctagt agaactctac tagcataaaa gaagcggtcc aaaaccttgc tcaagaagga
      121 ccgctttttg tagtgtaggg tgatatgaaa acggaatcct ggctgcaaaa gcacatcacc
      181 ttgcacgact atgatgaccc gaaagcggaa cgggcgaacg cgctgaccca cggcttcgga
      241 gcgatcctcg ccgtgttggc ccttggaatg gtcatcggac ggttctcctt ctactcatcc
      301 ggttccctcg cccggggaat ggtcctcttt tgcctgacga tgatccttct ctacagttca
      361 tcaacccttt atcacctcgc ccccaaaagt gatgccaaac ggatcttgcg agtcttcgac
      421 cactcgagca tctacgttct gattgccgga acctacacgc cgttgatgct cggaatcgac
      481 tccgatacgg caaggatggt cctctttctg gtatggacga tcgccgttgc cgggatcatc
      541 ctgacctttc tcttctgggg aaaattcaaa gtgcttcgcg ttcttttgta cgtcgtgatg
      601 ggttggctgg tcgtcttctt ctggggtgac atcatcccgt ttctgccgca aggccttttt
      661 ccctacgttc tggccggggg aatcacctac acggtggggg tggcgtttta tgcaagcaaa
      721 cgcctcccct gccatcatgc ggtctggcac ctcttctgca tcggaggaag cgtgccgttc
      781 tttatcggct ttttcgtgaa gcttcgctag tgatctcttc aatggcgtat tgaagatact
      841 gctcgttcac catccccaat tgacctccgg cgatcgctcc gtcaccgtag aagaagacag
      901 tcgtcggcaa gctggaggtc tggaagatgt aggcgaagat tccttcatcg tagaacaccg
      961 agctctcatc catcttctgc ttcctgatga aggttttcag cgtggcgagg ctctcccgct
     1021 ccccgtccag cgaatcgagg
//
LOCUS       DAOPUZ010000472         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_172221_length_1039_cov_2.502033, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000472 DAOPUZ010000000
VERSION     DAOPUZ010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172221_length_1039_cov_2.502033"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PKN79_06970"
     CDS             complement(<1..747)
                     /locus_tag="PKN79_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNZ95540.1"
                     /translation="MYIIGTAGHVDHGKSALVKALTATDPDRLPEEKERSLTIELGFA
                     SYTNKQGFVIGVIDVPGHERFIRNMVSGVWSLDGSLLCVAADDGWMQQSRDHALVLKA
                     MGARLPMGVITKSDLVSEERLSVVKKQVAKELQSIFGSEPIIHVISSKEGRGIEELKE
                     SIDSYLTKKKTTNFPPALALDRSFLIDGVGAVGTGSLRSMELKIGDEVTLLPSNLRAR
                     IRTLQSFGKNVERVKDGSRVAISLRGIEHDQ"
     gene            complement(747..>1039)
                     /locus_tag="PKN79_06975"
     CDS             complement(747..>1039)
                     /locus_tag="PKN79_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95541.1"
                     /translation="RKTGSSILERIKSERLSLIEDTMTLGGGSTPDENLPSLSIRLES
                     SKPQKDARTLRLRPVPIITTIHKGAVHINLGTVMYHETEELVSALTDLVEAP"
BASE COUNT          197 a          295 c          314 g          233 t
ORIGIN      
        1 ctgatcatgc tcgatccctc gaagggagat cgcaacccgg gagccgtcct tcacccgctc
       61 gacgttcttt ccgaacgatt ggagcgtccg gatccgggcg cggaggttcg atggcagaag
      121 agtcacttca tcaccgatct tcagctccat cgagcggagc gatccggtgc cgaccgcccc
      181 gacaccgtcg atcaggaagg agcggtcgag ggcgagggca ggaggaaagt tcgtggtctt
      241 ctttttcgtg aggtaggaat caatcgattc cttcagctct tcgatccctc gtccttcctt
      301 gctgctgatc acatggatga tcggttcgga accgaagatc gactgaagct ctttggcgac
      361 ctgcttcttc acgaccgaga ggcgttcttc ggaaacgagg tcgcttttgg tgatgacccc
      421 catcggaagg cgggcgccca tcgccttcag aacgagggcg tggtcgcggc tttgctgcat
      481 ccacccgtca tcggcggcga cgcacagcaa tgagccatcc agcgaccaga cgccgctgac
      541 catgttgcgg atgaagcgtt catggccggg cacgtcgatg accccgatca cgaagccctg
      601 cttgttcgta taggaagcga accccagttc gatcgtaagc gagcgttcct tctcttcggg
      661 gagtcggtcg gggtcggttg cggtcaatgc ctttaccaga gccgatttgc catggtccac
      721 atggccggcg gttccaataa tgtacactaa ggggcctcca ccaagtcggt aagcgctgaa
      781 accagctctt cagtctcatg atacattacc gttccaaggt tgatgtgaac agcccccttg
      841 tggatcgtgg tgatgatcgg gaccggtcgg aggcggaggg ttcgggcgtc tttctggggt
      901 ttggatgatt caagacggat cgaaagggag ggcaggtttt catccggggt cgatcctccc
      961 ccgagggtca tcgtgtcctc aatgagggag aggcgctcgc tcttgatcct ttccaggatc
     1021 gaacttcccg tctttcgca
//
LOCUS       DAOPUZ010000473         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_173336_length_1035_cov_2.266327, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000473 DAOPUZ010000000
VERSION     DAOPUZ010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173336_length_1035_cov_2.266327"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..590)
                     /locus_tag="PKN79_06980"
     CDS             complement(33..590)
                     /locus_tag="PKN79_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNZ95542.1"
                     /translation="MSLVLGVDFGTASVRSIVVDPHTGSILGSGEYAYQHTPLYPNPF
                     MVRHHLEDYRLGLEQSVKDAMKEVDASLIAALGLCSTGSSILPILEDGTALLDLPAHR
                     SNPNAHIWLWKDHTPTEEAGTINSLIHRSHPNYLSRIGSVYSAEWYWSKVLHCLHVDP
                     ELFDETYTWIEITDYLLFLLSGKRR"
     gene            complement(650..>1035)
                     /locus_tag="PKN79_06985"
     CDS             complement(650..>1035)
                     /locus_tag="PKN79_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNZ95543.1"
                     /translation="TVVAEIKALTGGHGVERAFDCSANNEGRATAIRATRKWGKIAFV
                     GEGGSVTFNPSEDMLHDQKTIYGSWVTSIWKMEDLVERLVRWNIHPEDLITHRFSLDD
                     ADKAYALMASGKCGKVCVCFDEELK"
BASE COUNT          213 a          278 c          321 g          223 t
ORIGIN      
        1 ggggagacgt atacgtggat tgaaatcacc gactacctcc tctttccgct taggagaaag
       61 aggaggtagt cggtgatttc aatccacgta tacgtctcgt cgaagagctc ggggtcgacg
      121 tggaggcagt gaagcacctt ggaccagtac cactcggcgg agtagaccga gccgatccgg
      181 gagaggtagt tgggatggga acggtggatg aggctgttga tcgtgcccgc ctcctcggtc
      241 ggggtatggt ctttccacag ccagatatga gcgtttggat tgcttcgatg ggcggggagg
      301 tccagaaggg cggttccgtc ttccaggatg gggagaatcg acgagccggt cgaacacaag
      361 cccaaggcgg caatcaggga ggcatcaacc tctttcattg cgtccttgac cgattgttca
      421 agaccgaggc ggtaatcctc cagatgatgg cggaccatga aggggttggg gtagaggggg
      481 gtatgttgat aggcatattc gccgctaccg aggatcgaac cggtatgggg atcaaccacg
      541 atcgagcgga cgctcgccgt tccgaaatcg acaccgagaa ccagactcat cccattcctc
      601 cctgcaaaag agagggtgaa tcatcacccc ctttgtaaaa accagctgct tatttcaatt
      661 cttcatcgaa gcagacacag acttttccac acttcccgct ggccatcagc gcataggctt
      721 tatccgcatc gtcgaggctg aagcggtggg tgatgaggtc ctcggggtgg atgttccatc
      781 ggacgaggcg ttcgaccaga tcctccatct tccagatcga ggtcacccag ctgccgtaga
      841 tcgttttctg atcgtggagc atatcttcgc tcgggttgaa ggtgacgctg ccaccctcgc
      901 cgacgaaggc gatcttgccc catttgcggg ttgcccggat cgcggtcgcc cgtccttcgt
      961 tgttggccga gcagtcgaat gcccgctcga cgccgtgacc gccggtgagc gccttgatct
     1021 cggcgaccac ggtgg
//
LOCUS       DAOPUZ010000474         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_175416_length_1027_cov_3.556584, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000474 DAOPUZ010000000
VERSION     DAOPUZ010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175416_length_1027_cov_3.556584"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKN79_06990"
     CDS             <1..363
                     /locus_tag="PKN79_06990"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95544.1"
                     /translation="CEESGFELRTRGLAARTLSLTLQWIDGKERMRSMTLANPIVLDA
                     QIAAHAEILLQALLTRRPHVLGCTFRLSRLEPEIQTIDLFDAEQEMKRQNLQAAVDRI
                     RNRYGISALSTCGAFHGK"
     gene            353..>1027
                     /locus_tag="PKN79_06995"
     CDS             353..>1027
                     /locus_tag="PKN79_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNZ95545.1"
                     /translation="MGSRIGIVSGYSLAWGVLSAEELMSHLKALGVAKTAICDRDTIA
                     GYPRLREAASDEGIELILGCALTEGKETIFAFVQDQSGYEELCSLLSKRYQNPVYSYL
                     EDLHGTSLHLAAASDVTIDAPHRYHALLPGRLKKRSSPYPLIALDDGFLRSEADRIVH
                     KTLVAIGTNTNVGNLRVETYCAKGRILLDQRSFEELFAPWPEAIATANSLIAYDPFVS
                     ADEMAAL"
BASE COUNT          223 a          303 c          283 g          218 t
ORIGIN      
        1 tgcgaagaaa gcggttttga attgcggact cggggcctcg ccgcccgaac cctttcgctt
       61 actcttcagt ggatcgacgg gaaggagcgg atgcgatcga tgacccttgc caacccgatc
      121 gttcttgacg cccaaattgc agcccatgcg gaaatcctct tgcaggccct tctgacccgc
      181 cggcctcatg tgcttggatg caccttcaga ctttcacgtc ttgagccgga aattcagacg
      241 atcgaccttt ttgacgctga acaagagatg aagcggcaaa acctccaagc tgcagtcgac
      301 cggatccgaa acaggtatgg gatctcggct ctttcaacat gcggggcctt ccatgggaag
      361 tagaatcggc attgtcagcg ggtactcgct ggcatggggc gtcctgtcgg cggaagagct
      421 gatgagccac ctcaaagctc tcggggttgc caagacggcg atctgcgatc gggatacgat
      481 cgccggttac ccccgcttga gggaggccgc ttccgatgaa gggatcgagc tgatcctggg
      541 ctgtgcgctt accgaaggaa aagagacgat ctttgccttc gtccaggatc aaagcgggta
      601 tgaagagctc tgctccctgt tgagtaaacg gtatcaaaac cccgtctact cctaccttga
      661 ggatcttcac ggaacctccc tccatcttgc cgccgcaagc gatgtgacga tcgacgcccc
      721 gcatcggtat catgcactgc tgcccggtcg actcaagaag cgttcatccc cctacccgct
      781 tatcgccctc gacgacggat tcctgagaag cgaagcggat cggatcgtac ataagacgct
      841 ggtcgcgatc ggaacgaaca caaacgtcgg caatcttcgt gtcgaaacct actgtgcaaa
      901 aggaaggatc ctgcttgatc aacgttcatt tgaagagctc ttcgctccgt ggccggaagc
      961 gatcgccact gcaaactccc tgatcgccta cgacccgttt gtttccgccg acgagatggc
     1021 ggcgctg
//
LOCUS       DAOPUZ010000475         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_175474_length_1027_cov_2.582305, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000475 DAOPUZ010000000
VERSION     DAOPUZ010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175474_length_1027_cov_2.582305"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKN79_07000"
     CDS             <1..412
                     /locus_tag="PKN79_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNZ95546.1"
                     /translation="SLLEENLLSFPQKRVSTIIRTLGVPASLTREICERSHLEPDRNA
                     GTLQKEERKLLCRTAGALSYKVNTTKGFKQAMATSGGVELSEVNRKTMESRLTPGLFF
                     CGEVLDYDGESGGFNIQAACSTAYLAARTIGKRT"
     gene            complement(487..>1027)
                     /locus_tag="PKN79_07005"
     CDS             complement(487..>1027)
                     /locus_tag="PKN79_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HNZ95547.1"
                     /translation="FGMDVVLAHPEGYNVMSEVEEVARKNAESNGGSYKRVDTMAEAF
                     KDADIVYPKSWAPFAVMEERTEIVEHNDQAGLKELEQRCLLNNAKFKDWTCTEELMAT
                     TKDGKALYMHCLPADITGVSCKEGEVEASVFDRYIVDLYKEASYKPYVIAAMIFLARF
                     KDPAAKLEQILEKGKQRIK"
BASE COUNT          212 a          300 c          284 g          231 t
ORIGIN      
        1 ctctctcctt gaagagaacc tcctgtcctt tccccaaaaa cgggtctcaa cgatcatcag
       61 gacattggga gtacccgcat cgttgacaag ggagatctgc gagcgatccc accttgagcc
      121 cgaccggaat gccggaacgc tccaaaagga ggagcggaag ctcctctgcc gcacggcggg
      181 tgcattatcc tacaaagtga acaccaccaa agggttcaag caggcgatgg cgacctccgg
      241 cggagtcgag ctttccgagg tcaacagaaa aacgatggaa tcgaggctaa cccccggcct
      301 cttcttctgc ggggaggttc tcgactatga cggagaaagc ggcggcttta acatccaggc
      361 ggcatgcagc accgcctatc tggctgctcg gacgatagga aaaagaacat aacatcatgg
      421 acatggatag cgtagaacaa ggagcctttc ggctccttgt cggtcgataa cgttgaatcg
      481 ttgcgcttat ttgatgcgct gctttccctt ttcaagaatc tgctcaagct tggccgccgg
      541 atccttgaag cgggcaagga agatcattgc cgcgatgacg tagggcttgt agctcgcttc
      601 cttgtagagg tcgacgatgt atcgatcgaa caccgatgct tcgacttccc cttccttgca
      661 ggagacgccg gtgatgtcgg cgggcagaca gtgcatgtag agcgccttgc cgtccttggt
      721 ggtggccatc agctcttcgg tgcaggtcca atccttgaac ttggcgttgt tcagcaggca
      781 gcgctgctcc agttccttca gcccggcctg gtcgttgtgc tcgacgatct cggtccgctc
      841 ttccatcacc gcgaacggcg cccagctctt cggatagacg atgtcggcat ccttgaacgc
      901 ttcagccatc gtgtcgaccc gcttgtatga tccaccgttg gattcggcgt tctttctggc
      961 gacttcctcg acctcgctca tcacgttgta cccttcgggg tgagcgagca cgacatccat
     1021 cccgaag
//
LOCUS       DAOPUZ010000476         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_176246_length_1024_cov_2.738906, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000476 DAOPUZ010000000
VERSION     DAOPUZ010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176246_length_1024_cov_2.738906"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PKN79_07010"
     CDS             <1..748
                     /locus_tag="PKN79_07010"
                     /inference="COORDINATES: protein motif:HMM:NF036726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95548.1"
                     /translation="DDETRLATLNDINLWIAHHAEANNGKQGLSRVFWVNRLVTARIL
                     QFGRLQFEERIFPYPATVYQDFGLEFIALSNGGLTLDRDGYPTKDENKITRTTVGREL
                     IDLSSGSIGEERIDASLLTKVLDEQSRVLFVHIPASGQLDPHLVEEALRKAEAFFGRG
                     WVYVCTSWLLDPALEKVVDSESNILSFNRRFSKLPVFFEYPQLFERVFSCNDEASVLS
                     ADATTSLQKNVQSALRRGVVFRTMGGFFLA"
     gene            776..>1024
                     /locus_tag="PKN79_07015"
     CDS             776..>1024
                     /locus_tag="PKN79_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ95549.1"
                     /translation="METPLHGLVDSHFHLLAIREKGIDPSPLLATMKELGYRGIDIGL
                     GGDDLAERIGLVGGYDEIYFATGLGPWAAAWEDWRVKEA"
BASE COUNT          218 a          267 c          291 g          248 t
ORIGIN      
        1 tgatgatgaa acccgtcttg caacactgaa tgacatcaat ctctggatcg cccaccacgc
       61 cgaagcgaat aacgggaagc agggtctttc ccgggtgttc tgggtcaatc gactggttac
      121 cgcccggatc ctccagttcg gacgccttca gtttgaggaa cggatctttc cctaccctgc
      181 gaccgtgtat caagattttg ggcttgagtt tatcgcactg agcaacggcg ggcttaccct
      241 cgatcgggac ggctacccca ccaaggatga gaataagatt actcgaacca ctgtcggtcg
      301 ggaattaatt gatctatcca gtgggtcgat cggtgaagaa cggattgatg cttcattgtt
      361 gaccaaggtc ttggatgagc aatcccgggt cctctttgtc catattcccg cttcagggca
      421 gcttgatcct catttggtgg aagaagcgct ccggaaggct gaagcatttt ttggaagagg
      481 atgggtatat gtttgcacct cctggctgct cgatcccgcc cttgagaagg tagtggactc
      541 cgaaagcaac atcctgtcat tcaaccggcg cttttcgaag ctgcccgtct tcttcgaata
      601 tccccaactc ttcgagcggg tcttctcctg caatgacgaa gcatcggttc tgtccgctga
      661 tgcgacgaca agtctgcaga agaacgtgca atcagcgctt cggcgcggag tcgtttttcg
      721 aacgatgggc ggattcttcc ttgcataaac gggtgggagc tgatactaca caggtatgga
      781 aacaccgctg cacggtcttg tcgactccca ctttcatctc cttgcgatcc gagagaaagg
      841 aatcgatcct tccccgctgc tcgccacgat gaaggagctc gggtatcggg gaatcgatat
      901 cggccttggt ggagacgacc ttgccgaacg gatcggactg gtcggagggt atgatgagat
      961 ctatttcgcc accggtctcg gtccgtgggc ggcggcctgg gaggattgga gggtcaagga
     1021 agcg
//
LOCUS       DAOPUZ010000477         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_178278_length_1017_cov_2.321206, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000477 DAOPUZ010000000
VERSION     DAOPUZ010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178278_length_1017_cov_2.321206"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKN79_07020"
     CDS             complement(<1..361)
                     /locus_tag="PKN79_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HNZ95550.1"
                     /translation="MQEAALFRSRTYHAIRSFFHERDYLEVDTPILSPTIIPESTIEN
                     FATRFRNPFLPSRDLYLLPSPEIYMKQLLAMGFGSIYQISHCFRNSEQLGRIHNIEFS
                     MLEYYSVGFDEEDSILLT"
     gene            428..>1017
                     /locus_tag="PKN79_07025"
     CDS             428..>1017
                     /locus_tag="PKN79_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNZ95551.1"
                     /translation="MSRYRTDDIIYALATPWGSAALAIIRISGSGSHALLAPYFSKSE
                     ALRSAKNATLLHGYLCDQEGRRIDEVVLAVNTKGHGYTLEEAVEITCHGSLSVVAELL
                     DFLSSIGMRSAEPGEFTFRAFLAGRMDLTQAEAVHELVGAQSPVTRAQALARLEGGLK
                     YKLDGMKDTLLTLLAPVEVQLDYSEDEIDEFVFPDEL"
BASE COUNT          231 a          254 c          308 g          224 t
ORIGIN      
        1 ccgtcagaag aatcgaatcc tcttcatcga agccgaccga gtaatactcc agcatggaaa
       61 attcgatatt atggattctt cctaattgct cgctgtttct gaaacagtgg ctgatctggt
      121 agatcgaccc gaagcccatc gcaagcaatt gcttcatgta gatctccggc gagggaagga
      181 gatagagatc ccgtgaagga agaaaggggt tcctgaaccg ggttgcgaag ttctcgatcg
      241 tggattcggg aataatggtc ggagagagga tcggggtatc cacttcaagg taatcccgtt
      301 catggaaaaa ggagcggatt gcgtgatagg tccgggatcg aaagagggcg gcttcctgca
      361 tgcaaacatt gtaccgatct gagctcttct gttgcaagct cccggtgcaa aaggatatac
      421 tcggcgcatg agtcgatatc ggaccgatga catcatctat gctctcgcca ccccgtgggg
      481 aagcgccgcc ctagcgatca ttcgcatcag cggcagcggc tcgcacgccc tgctggctcc
      541 atatttcagt aagagcgaag ccctccgttc ggcaaaaaac gctacgttgt tgcatggcta
      601 tctttgtgac caggagggga gacggattga tgaggtcgtc ctggcggtga atacaaaggg
      661 ccatggatac accctcgagg aagcggtcga gatcacctgt cacgggtcgc tcagcgtggt
      721 tgccgaactc cttgattttt tgtcttcgat cggaatgcgg agcgccgaac ccggggagtt
      781 cacgttccgc gcctttcttg ctgggaggat ggatctcacc caggccgagg cggttcacga
      841 gctggtcggg gcgcaaagcc cggtaacgcg ggctcaggcg ctcgcgcgct tggaaggagg
      901 gttgaagtac aagctcgacg gaatgaagga tactcttttg accttgcttg cgccggtgga
      961 agtgcagctc gactacagtg aagacgagat cgacgagttc gtcttccccg atgagct
//
LOCUS       DAOPUZ010000478         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_178768_length_1015_cov_2.978125, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000478 DAOPUZ010000000
VERSION     DAOPUZ010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178768_length_1015_cov_2.978125"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKN79_07030"
     CDS             <1..470
                     /locus_tag="PKN79_07030"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNZ95552.1"
                     /translation="SSVFNELTLIVSPIGGGKTRYLSNLNLPSPHCGVVTSSNIEKTI
                     YHIKDLAGGEERLLLSEAYLPNARRFGRFFVLEETFDWANERIISNLETSKAVVFDEI
                     GRIEIEGWGLKSSFLTALRTPAIEIYAAVRDTLLDQVLKTFDLEERIARIMHL"
     gene            536..>1015
                     /locus_tag="PKN79_07035"
     CDS             536..>1015
                     /locus_tag="PKN79_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNZ95553.1"
                     /translation="MNELFWFGMLLVNFAFILTAFRLWGKTGLFAWIPVSVIVANIQV
                     TKNVTLFGLEATLGNIVYATSFLATDILSEFFGKKEAYKAVGIGFFSLVAMTVLMQLA
                     LIFTPSPTDLVQGAMMTIFSLMPRIALASLIAYLASNVHDVWAFAYWKEKRPGRKTLW
                     "
BASE COUNT          225 a          272 c          271 g          247 t
ORIGIN      
        1 cttcctccgt gttcaatgag ctgaccctga tcgtatcccc gatcggaggc ggaaagacgc
       61 ggtatctttc caacctgaac cttccttccc cccattgcgg ggtggtcaca tcttccaaca
      121 ttgagaagac gatctatcac atcaaggacc tcgccggcgg agaagagcgc ctgctcctca
      181 gtgaagcata cctgcccaac gctcgaagat tcggccgctt cttcgtcctt gaagaaacct
      241 tcgactgggc gaacgaacgg atcatctcca acctcgaaac gtccaaagcc gtcgtcttcg
      301 atgagatcgg acggatcgag atcgaggggt gggggctgaa atcttccttt ctcacggctc
      361 ttcggacgcc ggcgatcgag atctacgccg ccgtccgcga cactctcctc gatcaagtac
      421 tcaagacatt tgacttggaa gaaagaatcg cacgaatcat gcacctatag aaagaggaga
      481 ataccggaaa ggtcttgtgt ttcttcggtc tcttgcggta tacccttcaa catatatgaa
      541 tgaattgttt tggttcggaa tgctcctcgt caacttcgcc ttcatcctga cggcgttccg
      601 gttgtgggga aagaccggat tgttcgcgtg gattcccgtc agtgtcatcg tggcgaacat
      661 ccaggtgacg aagaacgtaa cgctcttcgg cctggaagcg accttgggca atatcgtgta
      721 tgcgacctcg tttttggcca ccgacattct cagtgagttt ttcggaaaga aagaagcgta
      781 caaggcggtt ggaatcggat ttttcagtct ggtggcgatg accgtgctga tgcagcttgc
      841 gttgattttt accccgtcac cgacggatct ggtccaaggt gcgatgatga cgatcttttc
      901 cctgatgccg cggatcgccc tcgcttcgct gattgcatat ctggcaagca atgtccatga
      961 cgtgtgggcg ttcgcctact ggaaagaaaa gagaccgggg cgaaagacgc tctgg
//
LOCUS       DAOPUZ010000479         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_180791_length_1008_cov_2.894019, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000479 DAOPUZ010000000
VERSION     DAOPUZ010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180791_length_1008_cov_2.894019"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PKN79_07040"
     CDS             <1..714
                     /locus_tag="PKN79_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNZ95554.1"
                     /translation="MGMGAIDYAINNPDRRPGRLVVTDINQERLDRAAMLYTVEHAKK
                     NGVELHYLNTRDLEDADSVLMELSNGEGYDDVFVMAPVRALIEQADAILAKDGCLNFF
                     AGPERTDFTASLNFYNVHYASTHIVGTSGGNTDDLRESLKLMEQGLINPAGMVTHIGG
                     LSSVPQTVIDLPKIPGGKKMIYTHLDFPLTALEDFAEKGKKYPLFAKLDELVKKHNGL
                     WNAEAEAYLMEHCTMRIED"
     gene            717..>1008
                     /locus_tag="PKN79_07045"
     CDS             717..>1008
                     /locus_tag="PKN79_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HNZ95555.1"
                     /translation="MKKRIAIDLGASNGRVLVGNLEEFEVVHRFTTHNDQILGEFYWN
                     IQGLFSEIKTGLKKAFDCYPDEIVSIGIDTWGVDYALVDGNGSLVSLCYHYRD"
BASE COUNT          233 a          258 c          303 g          214 t
ORIGIN      
        1 atggggatgg gcgcgatcga ctatgcgatc aacaatcccg accgccggcc cggccgtctg
       61 gtcgtcaccg atatcaatca ggagcgcctc gatcgagccg ccatgctcta taccgtcgag
      121 catgcgaaga agaacggggt cgagctgcac tacctcaaca cccgggatct tgaagatgcc
      181 gacagcgtgt tgatggaact ttccaacggg gaagggtacg acgacgtgtt cgtcatggcc
      241 ccggttcggg ccctgatcga gcaggcggat gcgattctcg ccaaggacgg gtgcttgaac
      301 ttctttgccg gaccggagcg gaccgacttt accgcaagcc tcaacttcta caacgtgcac
      361 tacgcttcga cccatatcgt cggaacgagc ggtggaaaca ccgacgacct gcgcgagtcg
      421 ttgaagctga tggagcaggg cttgatcaat cccgccggaa tggtcaccca catcggcgga
      481 ctttcctcag ttcctcagac ggtgattgac cttccgaaga ttcccggcgg aaagaagatg
      541 atctacacac accttgactt cccgctcacc gcgttggaag attttgcgga gaaggggaag
      601 aaatacccgc tcttcgccaa gctggacgag ctggtgaaaa agcataatgg tctgtggaat
      661 gccgaagccg aggcatatct catggagcat tgcacgatga ggattgagga ttaaagatga
      721 agaagcgcat cgcgattgat ttgggggcaa gcaacgggcg ggttctcgtt gggaatttgg
      781 aagagttcga agtggttcac cgcttcacga cgcacaatga tcagattctc ggtgagttct
      841 actggaacat tcagggactg ttttcggaga tcaagaccgg tttgaagaag gcgtttgact
      901 gctatccgga tgagatcgtt tcgattggaa tcgacacgtg gggcgtggat tacgccctgg
      961 ttgacgggaa cgggagcctc gtttcgcttt gctaccacta tcgcgatg
//
LOCUS       DAOPUZ010000480         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_181364_length_1006_cov_2.737119, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000480 DAOPUZ010000000
VERSION     DAOPUZ010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181364_length_1006_cov_2.737119"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1006)
                     /gene="hisS"
                     /locus_tag="PKN79_07050"
     CDS             complement(<1..>1006)
                     /gene="hisS"
                     /locus_tag="PKN79_07050"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNZ95556.1"
                     /translation="TDQGGREVALRFDLTVPFARYLAQHEHELALPFKRYHIDKVWRG
                     ENPQKGRYREFTQCDFDIVGVDNAESDAEILSLMASSFEKMNVKHARFHVAHRGMFNT
                     LLGNLGVENLSVDILRLVDKLRKIGEQETEEQLRALVKNERTARAILSYISKEDEKEP
                     FETTLDRLVALLGGENEHTLRMRTIHSYLEAEGIEERFLFDPSITRGLDYYTGIVYET
                     FLEGSEQIGSVCSGGRYNDLASLYTKTHLPGVGSSIGLDRLLAALEGAYDYRASDVLI
                     LNTDKSRYPEAIRVAKQLREAGLKVALFFEPKKLPAQYKWAEANAIPTAIILDQDEEG
                     S"
BASE COUNT          185 a          279 c          312 g          230 t
ORIGIN      
        1 cgatccttcc tcatcttggt cgaggatgat cgccgtggga atcgcgttgg cttcggccca
       61 tttgtactgg gcggggagtt tcttcggttc aaaaaagagg gcgaccttca ggccggcttc
      121 ccgaagctgt ttcgccaccc tgatcgcttc cgggtagcgg ctcttgtcgg tgttgaggat
      181 cagcacgtcg cttgcccggt agtcatacgc accttcaagg gcggcaagga gccgatcgag
      241 gccgatcgag gagccgaccc cgggcaggtg ggtcttggtg tagagggacg caaggtcgtt
      301 gtaccgtccg ccgctgcaga ccgaaccgat ctgctccgaa ccttccagga aggtttcgta
      361 gacgatgccg gtatagtagt ccagcccccg ggtgatggag ggatcgaaga ggaagcgctc
      421 ttcaattcct tcagcttcca ggtaggagtg gatcgtccgc atccggaggg tgtgttcatt
      481 ttccccgcca aggagagcga caaggcgatc gagcgtagtc tcaaaaggtt ctttttcatc
      541 ctctttggag atgtatgaaa gaatggcccg ggcggtccgt tcgtttttca ccaacgcgcg
      601 aagctgctcc tcggtttctt gttcaccgat ctttctcagc ttgtccacga gccggagaat
      661 gtccacgctg agattctcga ctccgaggtt gccgagcagg gtgttgaaca tcccccggtg
      721 cgccacgtgg aaccgggcgt gcttgacgtt cattttttca aaggaggacg ccatcaggga
      781 aaggatttcc gcatcgcttt cggcgttgtc gaccccgacg atgtcgaagt cgcactgggt
      841 gaattcccga tagcggccct tttgagggtt ttccccccgc cacaccttgt cgatgtggta
      901 gcgtttgaag ggcagggcga gctcatgctc gtgctgagcg agatagcggg cgaacggaac
      961 ggtcagatcg aagcgaagcg ccacctcccg acccccctga tcggtg
//
LOCUS       DAOPUZ010000481         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_181918_length_1004_cov_3.623815, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000481 DAOPUZ010000000
VERSION     DAOPUZ010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181918_length_1004_cov_3.623815"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1004
                     /gene="htpG"
                     /locus_tag="PKN79_07055"
     CDS             <1..>1004
                     /gene="htpG"
                     /locus_tag="PKN79_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNZ95557.1"
                     /translation="KLKYLTLTDERLKSLDYNPRIDITFTEESPRTLTIRDNGLGMDE
                     EELSNNLGTIASSGTKKFLASLTEEQKKDSNLIGQFGVGFYSAFMVAKRIDVRSKKAG
                     TEQAYLWSSTGKGTFDISESELDGHGTEIVLHLNEDGETYASRWQIEQLIKKYSDHIA
                     YPIFLTYEAVTYDDKKKDKDGKAERKSETKVEQINSSSALWRRPKSELTDKDYNEFYK
                     QATYDSDDPLFYVHTKAEGTTEYTTLFFIPKKAPFDMYYADYRPGVKLYVKRVYITDD
                     DKELLPPYLRFVRGIIDSEDLPLNVSREILQQNRIMASIRSSSVKKLLGEFQKMSENN
                     P"
BASE COUNT          277 a          293 c          256 g          178 t
ORIGIN      
        1 aaactgaaat atctgaccct caccgacgag cggctgaaga gccttgatta caaccctcgg
       61 atcgatatca cctttaccga agaatctccc cggaccctca cgatccgtga caacggtctc
      121 ggaatggatg aggaagagct ttccaacaac ctgggaacga tcgcatccag cggaacgaag
      181 aagttcctcg cttccctgac ggaagaacag aagaaagact ccaacctgat cggtcagttc
      241 ggtgtcggat tctacagcgc cttcatggtc gccaagcgga tcgatgtccg ctccaagaaa
      301 gcgggaaccg agcaggcata cctttggtcc tcgaccggaa aaggcacgtt cgacatttcc
      361 gagagcgagc tggacggcca tggaacggaa atcgtcctcc acctcaacga agatggcgaa
      421 acatacgcca gccgctggca gatcgaacag cttataaaga agtacagcga ccacatcgcc
      481 tatccgatct ttctgaccta tgaagcggtg acctatgatg acaaaaagaa ggataaggac
      541 ggcaaagccg agaggaagag cgagaccaag gtcgaacaga tcaacagctc ttcggcgctc
      601 tggcgccggc cgaagagcga gttgaccgac aaggattaca acgagttcta caagcaggcc
      661 acctacgaca gcgacgatcc cctcttctat gtacacacca aggcggaggg aaccaccgag
      721 tacacgactc tcttcttcat ccccaagaaa gccccctttg atatgtacta cgccgactat
      781 cgccccgggg tgaagctcta tgtgaagcgg gtgtacatca ccgatgacga caaggagctg
      841 ctgcccccat atctccgttt cgtccgggga atcatcgaca gcgaggatct tcccctgaac
      901 gtctcgcggg aaattctcca gcagaaccgg atcatggcct cgatccgctc ctccagcgtg
      961 aagaagctgc tcggtgaatt ccagaagatg agcgagaaca accc
//
LOCUS       DAOPUZ010000482         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1405
            NODE_182543_length_1002_cov_2.862724, whole genome shotgun
            sequence.
ACCESSION   DAOPUZ010000482 DAOPUZ010000000
VERSION     DAOPUZ010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854610
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:32:37
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,413
            CDSs (total)                      :: 1,394
            Genes (coding)                    :: 1,374
            CDSs (with protein)               :: 1,374
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 9 of 20
            Pseudo Genes (incomplete)         :: 4 of 20
            Pseudo Genes (internal stop)      :: 7 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182543_length_1002_cov_2.862724"
                     /isolate="AMR_MDS_1405"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PKN79_07060"
     CDS             <1..183
                     /locus_tag="PKN79_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HNZ95558.1"
                     /translation="IVLGSLLSFAVTNLMDWSLHLSYASFILALGFSMFVGVFFGWYP
                     AVKASKLDPIDALNYE"
     gene            301..>1002
                     /locus_tag="PKN79_07065"
     CDS             301..>1002
                     /locus_tag="PKN79_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNZ95559.1"
                     /translation="MTSALYEREELRLQNEAERAFNSVFLALQDSNAKALRTMAEENV
                     SGVGVYSSMGRKVMSLGKVPMTIPAELFTTRDGFVRTGRATYNKATQMVEYIRFSRLT
                     ILLETGQLTLTEQGLLPTPIDFPDLLYLSLDGSAYHHRLMVIVVLGFFSGFVIIGLFI
                     AVLTIYLKNRSYRQTLAKQESLVNLGQAARTLAHEIKNPLSAVTIQVALLKKTLSEEH
                     KEDLQVIEGEVNRLTQ"
BASE COUNT          215 a          266 c          274 g          247 t
ORIGIN      
        1 atcgtgttgg gatcgctgct cagcttcgcc gtaaccaatt tgatggactg gtcgttgcat
       61 ttatcgtacg cctccttcat acttgcccta ggattctcga tgttcgtcgg ggtgtttttc
      121 ggctggtatc cggcagtgaa agcttccaaa ctcgacccga tcgatgcctt gaactatgaa
      181 taggagcata ctttgcgacg aagcagaaag cgattgatga taggcgatgg acgcgaagga
      241 gtcttcgtta tctcatcact gattttcggc tttcttttac ttcttggatt gcttgttttc
      301 ttgacctccg cgctgtatga gcgagaagaa cttcgccttc aaaacgaggc ggagcgggcg
      361 tttaactccg tgtttctcgc gctgcaggac agcaatgcca aagcgctgcg aacgatggcg
      421 gaagaaaacg taagcggggt aggggtctac tcctcgatgg ggcgcaaagt gatgagcttg
      481 ggcaaggtgc cgatgacaat ccccgccgag ctcttcacca cccgcgacgg gtttgtccgc
      541 accggacggg cgacgtacaa caaagcaacc cagatggttg agtatattcg gttcagccgg
      601 ttgacgattc tcttggagac cggtcaactc accctcaccg aacaggggct gcttccgact
      661 ccgatcgact ttcccgatct tctctatctg agtctcgacg ggtccgccta ccatcatcgg
      721 ttgatggtca ttgtggttct cggtttcttt tcaggcttcg tcatcatcgg cttgttcatc
      781 gccgtcctga cgatctatct gaaaaaccgc tcctaccggc aaacattggc caagcaggaa
      841 agcctcgtga acttggggca ggccgcccgg acgctggctc atgagatcaa gaatccgctc
      901 agtgcggtta cgattcaggt ggcgttgctt aagaaaacgc tctcggagga gcacaaagag
      961 gacctgcagg tcatcgaagg ggaagtgaac cgcctcaccc aa
//
